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Protein

Semaphorin-5A

Gene

Sema5a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Bifunctional axonal guidance cue regulated by sulfated proteoglycans; attractive effects result from interactions with heparan sulfate proteoglycans (HSPGs), while the inhibitory effects depend on interactions with chondroitin sulfate proteoglycans (CSPGs). Ligand for receptor PLXNB3. In glioma cells, SEMA5A stimulation of PLXNB3 results in the disassembly of F-actin stress fibers, disruption of focal adhesions and cellular collapse as well as inhibition of cell migration and invasion through ARHGDIA-mediated inactivation of RAC1 (By similarity). May promote angiogenesis by increasing endothelial cell proliferation and migration and inhibiting apoptosis.By similarity1 Publication

GO - Molecular functioni

  • axon guidance receptor activity Source: MGI
  • chondroitin sulfate proteoglycan binding Source: UniProtKB
  • heparan sulfate proteoglycan binding Source: UniProtKB
  • semaphorin receptor binding Source: UniProtKB
  • syndecan binding Source: UniProtKB

GO - Biological processi

  • axonal fasciculation Source: UniProtKB
  • axon extension Source: MGI
  • axon guidance Source: MGI
  • blood vessel endothelial cell proliferation involved in sprouting angiogenesis Source: UniProtKB
  • branching morphogenesis of an epithelial tube Source: MGI
  • cell chemotaxis Source: UniProtKB
  • detection of light stimulus involved in visual perception Source: MGI
  • diencephalon development Source: UniProtKB
  • negative regulation of axon extension involved in axon guidance Source: UniProtKB
  • negative regulation of cell adhesion Source: UniProtKB
  • negative regulation of endothelial cell apoptotic process Source: UniProtKB
  • neuron projection extension Source: MGI
  • neuron projection guidance Source: MGI
  • patterning of blood vessels Source: MGI
  • positive chemotaxis Source: UniProtKB
  • positive regulation of actin filament depolymerization Source: UniProtKB
  • positive regulation of angiogenesis Source: UniProtKB
  • positive regulation of axon extension involved in axon guidance Source: UniProtKB
  • positive regulation of catenin import into nucleus Source: UniProtKB
  • positive regulation of endothelial cell chemotaxis Source: UniProtKB
  • positive regulation of endothelial cell proliferation Source: UniProtKB
  • positive regulation of extracellular matrix disassembly Source: UniProtKB
  • positive regulation of protein kinase B signaling Source: UniProtKB
  • semaphorin-plexin signaling pathway Source: UniProtKB
  • signal clustering Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Neurogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Semaphorin-5A
Alternative name(s):
Semaphorin-F
Short name:
Sema F
Gene namesi
Name:Sema5a
Synonyms:Semaf, SemF
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:107556. Sema5a.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini22 – 971950ExtracellularSequence analysisAdd
BLAST
Transmembranei972 – 99221HelicalSequence analysisAdd
BLAST
Topological domaini993 – 107785CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • extracellular exosome Source: MGI
  • integral component of membrane Source: MGI
  • membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

Mutant mice die between E11.5 and E12.5. At this stage, no defects are detected in the development of extraembryonic tissues, cardiovascular system, axonal trajectories and peripheral nervous system. Mutants display decreased complexity of the hierarchically organized branches of the cranial blood vessels (PubMed:15743826). Mutant mice are viable and fertile (PubMed:21835343).2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Sequence analysisAdd
BLAST
Chaini22 – 10771056Semaphorin-5APRO_0000032336Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi104 ↔ 114By similarity
Disulfide bondi131 ↔ 140By similarity
Glycosylationi147 – 1471N-linked (GlcNAc...)Sequence analysis
Glycosylationi168 – 1681N-linked (GlcNAc...)Sequence analysis
Glycosylationi227 – 2271N-linked (GlcNAc...)Sequence analysis
Disulfide bondi254 ↔ 357By similarity
Glycosylationi277 – 2771N-linked (GlcNAc...)Sequence analysis
Disulfide bondi278 ↔ 320By similarity
Glycosylationi323 – 3231N-linked (GlcNAc...)Sequence analysis
Glycosylationi367 – 3671N-linked (GlcNAc...)Sequence analysis
Disulfide bondi487 ↔ 504By similarity
Disulfide bondi496 ↔ 513By similarity
Glycosylationi536 – 5361N-linked (GlcNAc...)Sequence analysis
Glycosylationi591 – 5911N-linked (GlcNAc...)Sequence analysis
Disulfide bondi607 ↔ 644By similarity
Disulfide bondi611 ↔ 650By similarity
Disulfide bondi622 ↔ 634By similarity
Disulfide bondi665 ↔ 696By similarity
Disulfide bondi669 ↔ 701By similarity
Disulfide bondi680 ↔ 686By similarity
Glycosylationi717 – 7171N-linked (GlcNAc...)Sequence analysis
Disulfide bondi796 ↔ 833By similarity
Disulfide bondi800 ↔ 838By similarity
Disulfide bondi811 ↔ 823By similarity
Disulfide bondi853 ↔ 890By similarity
Disulfide bondi857 ↔ 895By similarity
Disulfide bondi868 ↔ 880By similarity
Glycosylationi933 – 9331N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ62217.
PaxDbiQ62217.
PRIDEiQ62217.

PTM databases

iPTMnetiQ62217.
PhosphoSiteiQ62217.

Expressioni

Tissue specificityi

In adult, detected in liver, brain, kidney, heart, lung and spleen.

Developmental stagei

Differentially expressed in embryonic and adult tissues. Its abundance decreases from E10 to birth. At E10.5, detected in the atrial septum and endocardial cushions, and at lower levels in the atrial and ventricular endocardium. Strong expression detected in embryonic and postnatal retina. At P0 and P3, expression detected in the outer neuroblastic layer. After P7, the expression becomes more restricted and is observed in the middle to outer part of inner nuclear layer, and is not detectable at P21 when retinal development is almost complete.2 Publications

Interactioni

Subunit structurei

Binds PLXNB3.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000069024.

Structurei

3D structure databases

ProteinModelPortaliQ62217.
SMRiQ62217. Positions 68-506, 540-702, 783-940.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini35 – 484450SemaPROSITE-ProRule annotationAdd
BLAST
Domaini540 – 59354TSP type-1 1PROSITE-ProRule annotationAdd
BLAST
Domaini595 – 65157TSP type-1 2PROSITE-ProRule annotationAdd
BLAST
Domaini653 – 70250TSP type-1 3PROSITE-ProRule annotationAdd
BLAST
Domaini707 – 76559TSP type-1 4PROSITE-ProRule annotationAdd
BLAST
Domaini784 – 83956TSP type-1 5PROSITE-ProRule annotationAdd
BLAST
Domaini841 – 89656TSP type-1 6PROSITE-ProRule annotationAdd
BLAST
Domaini897 – 94448TSP type-1 7PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the semaphorin family.Curated
Contains 1 PSI domain.Curated
Contains 1 Sema domain.PROSITE-ProRule annotation
Contains 7 TSP type-1 domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3611. Eukaryota.
ENOG410XQZC. LUCA.
HOGENOMiHOG000047106.
HOVERGENiHBG062356.
InParanoidiQ62217.
PhylomeDBiQ62217.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR016201. Plexin-like_fold.
IPR001627. Semap_dom.
IPR027231. Semaphorin.
IPR000884. TSP1_rpt.
IPR015943. WD40/YVTN_repeat-like_dom.
[Graphical view]
PANTHERiPTHR11036. PTHR11036. 4 hits.
PfamiPF01403. Sema. 1 hit.
PF00090. TSP_1. 5 hits.
[Graphical view]
SMARTiSM00423. PSI. 1 hit.
SM00630. Sema. 1 hit.
SM00209. TSP1. 6 hits.
[Graphical view]
SUPFAMiSSF101912. SSF101912. 1 hit.
SSF103575. SSF103575. 1 hit.
SSF82895. SSF82895. 6 hits.
PROSITEiPS51004. SEMA. 1 hit.
PS50092. TSP1. 6 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q62217-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKGACILAWL FSSLGVWRLA RPETQDPAKC QRAEHPVVSY KEIGPWLREF
60 70 80 90 100
RAENAVDFSR LTFDPGQKEL VVGARNYLFR LELEDLSLIQ AVEWECDEAT
110 120 130 140 150
KKACYSKGKS KEECQNYIRV LLVGGDRLFT CGTNAFTPVC TIRSLSNLTE
160 170 180 190 200
IHDQISGMAR CPYSPQHNST ALLTASGELY AATAMDFPGR DPAIYRSLGT
210 220 230 240 250
LPPLRTAQYN SKWLNEPNFV SSYDIGNFTY FFFRENAVEH DCGKTVFSRP
260 270 280 290 300
ARVCKNDIGG RFLLEDTWTT FMKARLNCSR PGEVPFYYNE LQGTFFLPEL
310 320 330 340 350
DLIYGIFTTN VNSIASSAVC VFNLSAISQA FNGPFKYQEN SRSAWLPYPN
360 370 380 390 400
PNPNFQCGTM DQGLYVNLTE RNLQDAQKFI LMHEVVQPVT TVPSFMEDNS
410 420 430 440 450
RFSHLAVDVV QGRETLVHII YLGTDYGTIK KVRAPLSQSS GSCLLEEIEL
460 470 480 490 500
FPERRSEPIR SLQILHSQSV LFVGLQEHVA KIPLKRCHFH QTRSACIGAQ
510 520 530 540 550
DPYCGWDAVM KKCTSLEESL SMTQWDQSIP TCPTRNLTVD GSFGPWSPWT
560 570 580 590 600
PCTHTDGTAV GSCLCRSRSC DRPAPQCGGW QCEGPRMEIT NCSRNGGWTP
610 620 630 640 650
WTSWSPCSTT CGIGFQVRQR SCSNPTPRHG GRVCVGQNRE ERYCNEHLLC
660 670 680 690 700
PPHVFWTGWG PWERCTAQCG GGIQARRRTC ENGPDCAGSN VEYHPCNTNA
710 720 730 740 750
CPELKKTTPW TPWTPVNISD NGGHYEQRFR YTCKARLPDP NLLEVGRQRI
760 770 780 790 800
EMRYCSSDGT SGCSTDGLSG DFLRAGRYSA HTVNGAWSAW TSWSQCSRDC
810 820 830 840 850
SRGIRNRKRV CNNPEPKFGG MPCLGPSLEF QECNILPCPV DGVWSCWSSW
860 870 880 890 900
SKCSATCGGG HYMRTRSCSN PAPAYGGDIC LGLHTEEALC NTQTCPESWS
910 920 930 940 950
EWSDWSVCDA SGTQVRARQC ILLFPVGSQC SGNTTESRPC VFDSNFIPEV
960 970 980 990 1000
SVARSSSVEE KRCGEFNMFH MFHMMAVGLS SSILGCLLTL LVYTYCQRYQ
1010 1020 1030 1040 1050
QQSHDATVIH PVSPAALNSS ITNHINKLDK YDSVEAIKAF NKNNLILEER
1060 1070
NKYFNPHLTG KTYSNAYFTD LNNYDEY
Length:1,077
Mass (Da):120,826
Last modified:November 1, 1996 - v1
Checksum:iEDAB0DDDA42789FF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X97817 mRNA. Translation: CAA66397.1.
UniGeneiMm.260374.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X97817 mRNA. Translation: CAA66397.1.
UniGeneiMm.260374.

3D structure databases

ProteinModelPortaliQ62217.
SMRiQ62217. Positions 68-506, 540-702, 783-940.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000069024.

PTM databases

iPTMnetiQ62217.
PhosphoSiteiQ62217.

Proteomic databases

MaxQBiQ62217.
PaxDbiQ62217.
PRIDEiQ62217.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:107556. Sema5a.

Phylogenomic databases

eggNOGiKOG3611. Eukaryota.
ENOG410XQZC. LUCA.
HOGENOMiHOG000047106.
HOVERGENiHBG062356.
InParanoidiQ62217.
PhylomeDBiQ62217.

Miscellaneous databases

PROiQ62217.
SOURCEiSearch...

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR016201. Plexin-like_fold.
IPR001627. Semap_dom.
IPR027231. Semaphorin.
IPR000884. TSP1_rpt.
IPR015943. WD40/YVTN_repeat-like_dom.
[Graphical view]
PANTHERiPTHR11036. PTHR11036. 4 hits.
PfamiPF01403. Sema. 1 hit.
PF00090. TSP_1. 5 hits.
[Graphical view]
SMARTiSM00423. PSI. 1 hit.
SM00630. Sema. 1 hit.
SM00209. TSP1. 6 hits.
[Graphical view]
SUPFAMiSSF101912. SSF101912. 1 hit.
SSF103575. SSF103575. 1 hit.
SSF82895. SSF82895. 6 hits.
PROSITEiPS51004. SEMA. 1 hit.
PS50092. TSP1. 6 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "A novel class of murine semaphorins with homology to thrombospondin is differentially expressed during early embryogenesis."
    Adams R.H., Betz H., Pueschel A.W.
    Mech. Dev. 57:33-45(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: NMRI.
  2. "Inactivation of the Sema5a gene results in embryonic lethality and defective remodeling of the cranial vascular system."
    Fiore R., Rahim B., Christoffels V.M., Moorman A.F., Puschel A.W.
    Mol. Cell. Biol. 25:2310-2319(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
  3. "Semaphorin 5A promotes angiogenesis by increasing endothelial cell proliferation, migration, and decreasing apoptosis."
    Sadanandam A., Rosenbaugh E.G., Singh S., Varney M., Singh R.K.
    Microvasc. Res. 79:1-9(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. Cited for: DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiSEM5A_MOUSE
AccessioniPrimary (citable) accession number: Q62217
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: May 11, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.