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Q62191

- RO52_MOUSE

UniProt

Q62191 - RO52_MOUSE

Protein

E3 ubiquitin-protein ligase TRIM21

Gene

Trim21

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 122 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    E3 ubiquitin-protein ligase whose activity is dependent on E2 enzymes, UBE2D1, UBE2D2, UBE2E1 and UBE2E2. Forms a ubiquitin ligase complex in cooperation with the E2 UBE2D2 that is used not only for the ubiquitination of USP4 and IKBKB but also for its self-ubiquitination. Component of cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes such as SCF(SKP2)-like complexes. A TRIM21-containing SCF(SKP2)-like complex is shown to mediate ubiquitination of CDKN1B ('Thr-187' phosphorylated-form), thereby promoting its degradation by the proteasome. Monoubiquitinates IKBKB that will negatively regulates Tax-induced NF-kappa-B signaling. Negatively regulates IFN-beta production post-pathogen recognition by polyubiquitin-mediated degradation of IRF3. Mediates the ubiquitin-mediated proteasomal degradation of IgG1 heavy chain, which is linked to the VCP-mediated ER-associated degradation (ERAD) pathway. Promotes IRF8 ubiquitination, which enhanced the ability of IRF8 to stimulate cytokine genes transcription in macrophages. Plays a role in the regulation of the cell cycle progression. Enhances the decapping activity of DCP2. Exists as a ribonucleoprotein particle present in all mammalian cells studied and composed of a single polypeptide and one of four small RNA molecules. At least two isoforms are present in nucleated and red blood cells, and tissue specific differences in RO/SSA proteins have been identified. The common feature of these proteins is their ability to bind HY RNAs.2.1 Publication

    Pathwayi

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri20 – 5940RING-typePROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri96 – 12732B box-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. ligase activity Source: UniProtKB-KW
    3. protein binding Source: IntAct
    4. RNA binding Source: UniProtKB-KW
    5. ubiquitin-protein transferase activity Source: UniProtKB
    6. zinc ion binding Source: InterPro

    GO - Biological processi

    1. cell cycle Source: UniProtKB-KW
    2. negative regulation of NF-kappaB transcription factor activity Source: UniProtKB
    3. negative regulation of protein deubiquitination Source: UniProtKB
    4. positive regulation of cell cycle Source: UniProtKB
    5. protein autoubiquitination Source: UniProtKB
    6. protein destabilization Source: UniProtKB
    7. protein monoubiquitination Source: UniProtKB
    8. protein polyubiquitination Source: UniProtKB
    9. protein ubiquitination Source: UniProtKB

    Keywords - Molecular functioni

    Ligase, Ribonucleoprotein

    Keywords - Biological processi

    Cell cycle, Ubl conjugation pathway

    Keywords - Ligandi

    DNA-binding, Metal-binding, RNA-binding, Zinc

    Enzyme and pathway databases

    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    E3 ubiquitin-protein ligase TRIM21 (EC:6.3.2.-)
    Alternative name(s):
    52 kDa Ro protein
    52 kDa ribonucleoprotein autoantigen Ro/SS-A
    Ro(SS-A)
    Sjoegren syndrome type A antigen
    Short name:
    SS-A
    Tripartite motif-containing protein 21
    Gene namesi
    Name:Trim21
    Synonyms:Ro52, Ssa1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Unplaced

    Organism-specific databases

    MGIiMGI:106657. Trim21.

    Subcellular locationi

    Cytoplasm 1 Publication. Nucleus 1 Publication. CytoplasmP-body By similarity
    Note: Enters the nucleus upon exposure to nitric oxide By similarity. Localizes to small dot- or rod-like structures in the cytoplasm, called cytoplasmic bodies (P-body) that are located underneath the plasma membrane and also diffusely in the cytoplasm and are highly motil in cells. Cytoplasmic bodies are located along the microtubules and do not share the same cytoplasmic bodies with TRIM5. Colocalizes with DCP2 in P-body.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. cytoplasmic mRNA processing body Source: UniProtKB-SubCell
    3. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 470470E3 ubiquitin-protein ligase TRIM21PRO_0000056116Add
    BLAST

    Post-translational modificationi

    Autoubiquitinated; does not lead to its proteasomal degradation. Deubiquitinated by USP4; leading to its stabilization By similarity. Autoubiquitinated.By similarity1 Publication

    Keywords - PTMi

    Ubl conjugation

    Proteomic databases

    PaxDbiQ62191.
    PRIDEiQ62191.

    PTM databases

    PhosphoSiteiQ62191.

    Expressioni

    Inductioni

    Up-regulated by IFN.1 Publication

    Gene expression databases

    CleanExiMM_TRIM21.
    GenevestigatoriQ62191.

    Interactioni

    Subunit structurei

    Component of a SCF(SKP2)-like complex containing CUL1, SKP1, TRIM21 and SKP2. Interacts with CALR, CUL1, FBXW11, HSPA5, IKBKB, IRF3, SKP1 and VCP. Interacts with SKP2; the interaction with SKP2 does not depend on an intact F-box domain. Interacts (via N-terminus and C-terminus) with DCP2 (via N-terminus and C-terminus) By similarity. Interacts (via C-terminus) with IRF8 (via C-terminus).By similarity1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Ddx41Q91VN66EBI-6840982,EBI-2551902

    Protein-protein interaction databases

    IntActiQ62191. 21 interactions.

    Structurei

    Secondary structure

    1
    470
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi297 – 2993
    Beta strandi304 – 3063
    Beta strandi312 – 3154
    Beta strandi331 – 3388
    Beta strandi341 – 35111
    Beta strandi358 – 3647
    Turni377 – 3804
    Beta strandi381 – 3877
    Beta strandi390 – 3934
    Beta strandi395 – 3973
    Beta strandi399 – 4013
    Beta strandi408 – 4158
    Turni416 – 4194
    Beta strandi420 – 4256
    Turni426 – 4305
    Beta strandi431 – 4366
    Beta strandi445 – 4506
    Beta strandi455 – 4573
    Beta strandi463 – 4653

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2VOKX-ray1.30A/B291-470[»]
    3ZO0X-ray1.99B291-470[»]
    ProteinModelPortaliQ62191.
    SMRiQ62191. Positions 4-78, 95-131, 291-468.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ62191.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini272 – 470199B30.2/SPRYPROSITE-ProRule annotationAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili188 – 25063Sequence AnalysisAdd
    BLAST

    Domaini

    The coiled-coil is necessary for the cytoplasmic localization. The B30.2/SPRY domain is necessary for the cytoplasmic localization, the interaction with IRF3 and for the IRF3-driven interferon beta promoter activity. The RING-type zinc finger is necessary for ubiquitination and for the IRF3-driven interferon beta promoter activity. Interacts with SKP2 and CUL1 in a RING finger-independent manner By similarity.By similarity

    Sequence similaritiesi

    Belongs to the TRIM/RBCC family.Curated
    Contains 1 B box-type zinc finger.PROSITE-ProRule annotation
    Contains 1 B30.2/SPRY domain.PROSITE-ProRule annotation
    Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri20 – 5940RING-typePROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri96 – 12732B box-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Coiled coil, Zinc-finger

    Phylogenomic databases

    eggNOGiNOG276395.
    HOGENOMiHOG000234134.
    HOVERGENiHBG001357.
    InParanoidiQ62191.
    PhylomeDBiQ62191.

    Family and domain databases

    Gene3Di3.30.40.10. 1 hit.
    4.10.45.10. 1 hit.
    InterProiIPR001870. B30.2/SPRY.
    IPR003879. Butyrophylin.
    IPR008985. ConA-like_lec_gl_sf.
    IPR006574. PRY.
    IPR018355. SPla/RYanodine_receptor_subgr.
    IPR003877. SPRY_rcpt.
    IPR000315. Znf_B-box.
    IPR020457. Znf_B-box_chordata.
    IPR018957. Znf_C3HC4_RING-type.
    IPR001841. Znf_RING.
    IPR013083. Znf_RING/FYVE/PHD.
    IPR017907. Znf_RING_CS.
    [Graphical view]
    PfamiPF13765. PRY. 1 hit.
    PF00622. SPRY. 1 hit.
    PF00643. zf-B_box. 1 hit.
    PF00097. zf-C3HC4. 1 hit.
    [Graphical view]
    PRINTSiPR01406. BBOXZNFINGER.
    PR01407. BUTYPHLNCDUF.
    SMARTiSM00336. BBOX. 1 hit.
    SM00589. PRY. 1 hit.
    SM00184. RING. 1 hit.
    SM00449. SPRY. 1 hit.
    [Graphical view]
    SUPFAMiSSF49899. SSF49899. 1 hit.
    PROSITEiPS50188. B302_SPRY. 1 hit.
    PS50119. ZF_BBOX. 1 hit.
    PS00518. ZF_RING_1. 1 hit.
    PS50089. ZF_RING_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q62191-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSPSTTSKMS LEKMWEEVTC SICLDPMVEP MSIECGHCFC KECIFEVGKN    50
    GGSSCPECRQ QFLLRNLRPN RHIANMVENL KQIAQNTKKS TQETHCMKHG 100
    EKLHLFCEED GQALCWVCAQ SGKHRDHTRV PIEEAAKVYQ EKIHVVLEKL 150
    RKGKELAEKM EMDLTMQRTD WKRNIDTQKS RIHAEFALQN SLLAQEEQRQ 200
    LQRLEKDQRE YLRLLGKKEA ELAEKNQALQ ELISELERRI RGSELELLQE 250
    VRIILERSGS WNLDTLDIDA PDLTSTCPVP GRKKMLRTCW VHITLDRNTA 300
    NSWLIISKDR RQVRMGDTHQ NVSDNKERFS NYPMVLGAQR FSSGKMYWEV 350
    DVTQKEAWDL GVCRDSVQRK GQFSLSPENG FWTIWLWQDS YEAGTSPQTT 400
    LHIQVPPCQI GIFVDYEAGV VSFYNITDHG SLIYTFSECV FAGPLRPFFN 450
    VGFNYSGGNA APLKLCPLKM 470
    Length:470
    Mass (Da):54,175
    Last modified:November 1, 1996 - v1
    Checksum:i393AE5AFD254855B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L27990 mRNA. Translation: AAB51154.1.
    UniGeneiMm.321227.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L27990 mRNA. Translation: AAB51154.1 .
    UniGenei Mm.321227.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2VOK X-ray 1.30 A/B 291-470 [» ]
    3ZO0 X-ray 1.99 B 291-470 [» ]
    ProteinModelPortali Q62191.
    SMRi Q62191. Positions 4-78, 95-131, 291-468.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q62191. 21 interactions.

    PTM databases

    PhosphoSitei Q62191.

    Proteomic databases

    PaxDbi Q62191.
    PRIDEi Q62191.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Organism-specific databases

    MGIi MGI:106657. Trim21.

    Phylogenomic databases

    eggNOGi NOG276395.
    HOGENOMi HOG000234134.
    HOVERGENi HBG001357.
    InParanoidi Q62191.
    PhylomeDBi Q62191.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .

    Miscellaneous databases

    EvolutionaryTracei Q62191.
    PROi Q62191.
    SOURCEi Search...

    Gene expression databases

    CleanExi MM_TRIM21.
    Genevestigatori Q62191.

    Family and domain databases

    Gene3Di 3.30.40.10. 1 hit.
    4.10.45.10. 1 hit.
    InterProi IPR001870. B30.2/SPRY.
    IPR003879. Butyrophylin.
    IPR008985. ConA-like_lec_gl_sf.
    IPR006574. PRY.
    IPR018355. SPla/RYanodine_receptor_subgr.
    IPR003877. SPRY_rcpt.
    IPR000315. Znf_B-box.
    IPR020457. Znf_B-box_chordata.
    IPR018957. Znf_C3HC4_RING-type.
    IPR001841. Znf_RING.
    IPR013083. Znf_RING/FYVE/PHD.
    IPR017907. Znf_RING_CS.
    [Graphical view ]
    Pfami PF13765. PRY. 1 hit.
    PF00622. SPRY. 1 hit.
    PF00643. zf-B_box. 1 hit.
    PF00097. zf-C3HC4. 1 hit.
    [Graphical view ]
    PRINTSi PR01406. BBOXZNFINGER.
    PR01407. BUTYPHLNCDUF.
    SMARTi SM00336. BBOX. 1 hit.
    SM00589. PRY. 1 hit.
    SM00184. RING. 1 hit.
    SM00449. SPRY. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49899. SSF49899. 1 hit.
    PROSITEi PS50188. B302_SPRY. 1 hit.
    PS50119. ZF_BBOX. 1 hit.
    PS00518. ZF_RING_1. 1 hit.
    PS50089. ZF_RING_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structural differences between the human and mouse 52-kD Ro autoantigens associated with poorly conserved autoantibody activity across species."
      Keech C.L., Gordon T.P., McCluskey J.
      Clin. Exp. Immunol. 104:255-263(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Macrophage.
    2. "Autoantigen Ro52 is an interferon inducible E3 ligase that ubiquitinates IRF-8 and enhances cytokine expression in macrophages."
      Kong H.J., Anderson D.E., Lee C.H., Jang M.K., Tamura T., Tailor P., Cho H.K., Cheong J., Xiong H., Morse H.C. III, Ozato K.
      J. Immunol. 179:26-30(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 226-237; 242-252; 298-308 AND 329-340, FUNCTION, INTERACTION WITH IRF8, AUTOUBIQUITINATION, SUBCELLULAR LOCATION, INDUCTION, IDENTIFICATION BY MASS SPECTROMETRY.

    Entry informationi

    Entry nameiRO52_MOUSE
    AccessioniPrimary (citable) accession number: Q62191
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 122 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3