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Q62187

- TTF1_MOUSE

UniProt

Q62187 - TTF1_MOUSE

Protein

Transcription termination factor 1

Gene

Ttf1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 115 (01 Oct 2014)
      Sequence version 2 (03 Oct 2006)
      Previous versions | rss
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    Functioni

    Multifunctional nucleolar protein that terminates ribosomal gene transcription, mediates replication fork arrest and regulates RNA polymerase I transcription on chromatin. Plays a dual role in rDNA regulation, being involved in both activation and silencing of rDNA transcription. Interaction with BAZ2A/TIP5 recovers DNA-binding activity.4 Publications

    GO - Molecular functioni

    1. chromatin binding Source: MGI
    2. DNA binding Source: MGI
    3. protein binding Source: UniProtKB

    GO - Biological processi

    1. chromatin remodeling Source: MGI
    2. negative regulation of DNA replication Source: UniProtKB-KW
    3. regulation of transcription, DNA-templated Source: UniProtKB-KW
    4. termination of RNA polymerase I transcription Source: MGI
    5. transcription initiation from RNA polymerase I promoter Source: MGI

    Keywords - Molecular functioni

    DNA replication inhibitor

    Keywords - Biological processi

    Transcription, Transcription regulation, Transcription termination

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    ReactomeiREACT_200667. NoRC negatively regulates rRNA expression.
    REACT_214440. NoRC negatively regulates rRNA expression.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transcription termination factor 1
    Short name:
    TTF-1
    Alternative name(s):
    RNA polymerase I termination factor
    Transcription termination factor I
    Short name:
    TTF-I
    Short name:
    mTFF-I
    Gene namesi
    Name:Ttf1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:105044. Ttf1.

    Subcellular locationi

    Nucleus 1 Publication. Nucleusnucleolus 1 Publication

    GO - Cellular componenti

    1. nucleolus Source: UniProtKB-SubCell
    2. nucleus Source: MGI

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 859859Transcription termination factor 1PRO_0000250473Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei227 – 2271PhosphoserineBy similarity
    Modified residuei375 – 3751PhosphoserineBy similarity
    Modified residuei451 – 4511PhosphoserineBy similarity
    Modified residuei457 – 4571Phosphoserine1 Publication
    Modified residuei460 – 4601Phosphoserine1 Publication
    Modified residuei845 – 8451PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ62187.
    PRIDEiQ62187.

    Expressioni

    Gene expression databases

    BgeeiQ62187.
    CleanExiMM_TTF1.
    GenevestigatoriQ62187.

    Interactioni

    Subunit structurei

    Oligomer. The oligomeric structure enables to interact simultaneously with two separatee DNA fragments. Interacts with BAZ2A/TIP5.2 Publications

    Protein-protein interaction databases

    BioGridi204361. 2 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ62187.
    SMRiQ62187. Positions 586-640, 694-719.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini585 – 63450Myb-like 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini634 – 71885Myb-like 2PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 210210N-terminal region (NRD)Add
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi359 – 36810Poly-Lys

    Domaini

    The N-terminal region (NRD) inhibits DNA-binding via its interaction with the C-terminal region.

    Sequence similaritiesi

    Contains 2 Myb-like domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG262979.
    GeneTreeiENSGT00530000063659.
    HOGENOMiHOG000231811.
    HOVERGENiHBG084694.
    InParanoidiQ62187.
    KOiK15225.
    OMAiTLESEWP.
    OrthoDBiEOG7ZKS9N.
    PhylomeDBiQ62187.
    TreeFamiTF333537.

    Family and domain databases

    Gene3Di1.10.10.60. 2 hits.
    InterProiIPR009057. Homeodomain-like.
    IPR017877. Myb-like_dom.
    IPR001005. SANT/Myb.
    [Graphical view]
    SMARTiSM00717. SANT. 2 hits.
    [Graphical view]
    PROSITEiPS50090. MYB_LIKE. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q62187-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKGGTSKFKT HTETLYKKKK WSSVSEKRPQ KCPSQCLESK QPQVSVLGKR    50
    RRASQTPAQE TLESEWPQKA KRKKRRREPQ TPAQETLESE WPQKAKKKKR 100
    RGEPQTPTQE SLESEQPPVS LLGKRRRESQ TPAQENSESE QPRKAKRRRK 150
    KRKGSQQPTS SLLKTPETFL KAKKTTSAHK KKKNSVLEVD METGIILVDK 200
    ENMENLLETS RKDVDIVYVD MSKGQRSAKV RETGELPAAK PQEHGCRELL 250
    GDVRSRKKQK HLQKVAPWDV VQGSQPESIS LPPSEPLSSE DLEGKSTEAA 300
    VFCKKKSKKN VFRSQELEPI PDSLDDSETI SERLDSTHHG GAVGAGEECE 350
    STKESHSIKK KSKKKKHKSV ALATSSDSAS VTDSKAKNAL VDSSEGSGAV 400
    REEDVDHRPA EAEAQACSTE KHREAMQRLE PTHEEESNSE SASNSAARHI 450
    SEDRRESDDS DVDLGSAVRQ LREFIPDIQE RAATTIRRMY RDDLGRFKEF 500
    KAQGVAIRFG KFSAKENKQI EKNVQDFLSL TGIESADKLL YTDRYPEEKT 550
    LITNLKRKHA FRLHIGKGIA RPWKLVYYRA KKIFDVNNYK GRYNEEDTKK 600
    LKAYHSLHGN DWKKIGAMVA RSSLSVALKF SQIGGTRNQG AWSKAETQRL 650
    IKAVEDVILK KMSPQELREL DSKLQEDPEG RLSIVREKLY KGISWVEVEA 700
    RVETRNWMQC KSKWTEILTK RMTHGGFVYR GVNALQAKIT LIERLYELNV 750
    NDANEIDWED LASAIGDVPP PFVQAKFYKL KAACVPFWQK KTFPEIIDYL 800
    YKNSLPLLKE KLDKKMKKKD GQIQTPAAPK QDFLFKDIFH CDDDSDEGSP 850
    EEPSASDVQ 859
    Length:859
    Mass (Da):97,723
    Last modified:October 3, 2006 - v2
    Checksum:iD47950ECB3A08DCD
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti72 – 9625Missing in CAA58808. (PubMed:7720715)CuratedAdd
    BLAST
    Sequence conflicti305 – 3073KKS → RSL in CAA58808. (PubMed:7720715)Curated
    Sequence conflicti348 – 3481Missing in CAA58808. (PubMed:7720715)Curated
    Sequence conflicti496 – 4961R → L AA sequence (PubMed:7720715)Curated
    Sequence conflicti762 – 7621A → C in CAA58808. (PubMed:7720715)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X83974 mRNA. Translation: CAA58808.1.
    AF237703 mRNA. Translation: AAF43448.1.
    BC059011 mRNA. Translation: AAH59011.1.
    CCDSiCCDS38089.1.
    PIRiS54776.
    RefSeqiNP_033468.2. NM_009442.2.
    XP_006497915.1. XM_006497852.1.
    UniGeneiMm.252195.

    Genome annotation databases

    EnsembliENSMUST00000100237; ENSMUSP00000097809; ENSMUSG00000026803.
    GeneIDi22130.
    KEGGimmu:22130.
    UCSCiuc008izk.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X83974 mRNA. Translation: CAA58808.1 .
    AF237703 mRNA. Translation: AAF43448.1 .
    BC059011 mRNA. Translation: AAH59011.1 .
    CCDSi CCDS38089.1.
    PIRi S54776.
    RefSeqi NP_033468.2. NM_009442.2.
    XP_006497915.1. XM_006497852.1.
    UniGenei Mm.252195.

    3D structure databases

    ProteinModelPortali Q62187.
    SMRi Q62187. Positions 586-640, 694-719.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 204361. 2 interactions.

    Proteomic databases

    PaxDbi Q62187.
    PRIDEi Q62187.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000100237 ; ENSMUSP00000097809 ; ENSMUSG00000026803 .
    GeneIDi 22130.
    KEGGi mmu:22130.
    UCSCi uc008izk.1. mouse.

    Organism-specific databases

    CTDi 7270.
    MGIi MGI:105044. Ttf1.

    Phylogenomic databases

    eggNOGi NOG262979.
    GeneTreei ENSGT00530000063659.
    HOGENOMi HOG000231811.
    HOVERGENi HBG084694.
    InParanoidi Q62187.
    KOi K15225.
    OMAi TLESEWP.
    OrthoDBi EOG7ZKS9N.
    PhylomeDBi Q62187.
    TreeFami TF333537.

    Enzyme and pathway databases

    Reactomei REACT_200667. NoRC negatively regulates rRNA expression.
    REACT_214440. NoRC negatively regulates rRNA expression.

    Miscellaneous databases

    NextBioi 302001.
    PROi Q62187.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q62187.
    CleanExi MM_TTF1.
    Genevestigatori Q62187.

    Family and domain databases

    Gene3Di 1.10.10.60. 2 hits.
    InterProi IPR009057. Homeodomain-like.
    IPR017877. Myb-like_dom.
    IPR001005. SANT/Myb.
    [Graphical view ]
    SMARTi SM00717. SANT. 2 hits.
    [Graphical view ]
    PROSITEi PS50090. MYB_LIKE. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Different domains of the murine RNA polymerase I-specific termination factor mTTF-I serve distinct functions in transcription termination."
      Evers R., Smid A., Rudloff U., Lottspeich F., Gummt I.
      EMBO J. 14:1248-1256(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 394-400 AND 457-498, FUNCTION, SUBCELLULAR LOCATION.
      Tissue: Ehrlich ascites tumor cell.
    2. Hu Q., Rothblum L.
      Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Brain.
    4. "Oligomerization of the transcription termination factor TTF-I: implications for the structural organization of ribosomal transcription units."
      Sander E.E., Grummt I.
      Nucleic Acids Res. 25:1142-1147(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBUNIT.
    5. "Termination of mammalian rDNA replication: polar arrest of replication fork movement by transcription termination factor TTF-I."
      Gerber J.-K., Goegel E., Berger C., Wallisch M., Mueller F., Grummt I., Grummt F.
      Cell 90:559-567(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "RNA polymerase I transcription on nucleosomal templates: the transcription termination factor TTF-I induces chromatin remodeling and relieves transcriptional repression."
      Laengst G., Blank T.A., Becker P.B., Grummt I.
      EMBO J. 16:760-768(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "The chromatin remodeling complex NoRC and TTF-I cooperate in the regulation of the mammalian rRNA genes in vivo."
      Nemeth A., Strohner R., Grummt I., Laengst G.
      Nucleic Acids Res. 32:4091-4099(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH BAZ2A, FUNCTION.
    8. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-457 AND SER-460, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.

    Entry informationi

    Entry nameiTTF1_MOUSE
    AccessioniPrimary (citable) accession number: Q62187
    Secondary accession number(s): Q9JKK5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 3, 2006
    Last sequence update: October 3, 2006
    Last modified: October 1, 2014
    This is version 115 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3