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Protein

Transcription termination factor 1

Gene

Ttf1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Multifunctional nucleolar protein that terminates ribosomal gene transcription, mediates replication fork arrest and regulates RNA polymerase I transcription on chromatin. Plays a dual role in rDNA regulation, being involved in both activation and silencing of rDNA transcription. Interaction with BAZ2A/TIP5 recovers DNA-binding activity.4 Publications

GO - Molecular functioni

  1. chromatin binding Source: MGI
  2. DNA binding Source: MGI

GO - Biological processi

  1. chromatin remodeling Source: MGI
  2. negative regulation of DNA replication Source: UniProtKB-KW
  3. regulation of transcription, DNA-templated Source: UniProtKB-KW
  4. termination of RNA polymerase I transcription Source: MGI
  5. transcription initiation from RNA polymerase I promoter Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

DNA replication inhibitor

Keywords - Biological processi

Transcription, Transcription regulation, Transcription termination

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiREACT_200667. NoRC negatively regulates rRNA expression.
REACT_214440. NoRC negatively regulates rRNA expression.
REACT_235780. RNA Polymerase I Transcription Initiation.
REACT_257165. RNA Polymerase I Transcription Termination.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription termination factor 1
Short name:
TTF-1
Alternative name(s):
RNA polymerase I termination factor
Transcription termination factor I
Short name:
TTF-I
Short name:
mTFF-I
Gene namesi
Name:Ttf1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 2

Organism-specific databases

MGIiMGI:105044. Ttf1.

Subcellular locationi

Nucleus 1 Publication. Nucleusnucleolus 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: Ensembl
  2. nucleolus Source: UniProtKB-SubCell
  3. nucleus Source: MGI
  4. plasma membrane Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 859859Transcription termination factor 1PRO_0000250473Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei227 – 2271PhosphoserineBy similarity
Modified residuei375 – 3751PhosphoserineBy similarity
Modified residuei451 – 4511PhosphoserineBy similarity
Modified residuei457 – 4571Phosphoserine1 Publication
Modified residuei460 – 4601Phosphoserine1 Publication
Modified residuei845 – 8451PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ62187.
PaxDbiQ62187.
PRIDEiQ62187.

Expressioni

Gene expression databases

BgeeiQ62187.
CleanExiMM_TTF1.
GenevestigatoriQ62187.

Interactioni

Subunit structurei

Oligomer. The oligomeric structure enables to interact simultaneously with two separatee DNA fragments. Interacts with BAZ2A/TIP5.2 Publications

Protein-protein interaction databases

BioGridi204361. 2 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ62187.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini585 – 63450Myb-like 1PROSITE-ProRule annotationAdd
BLAST
Domaini634 – 71885Myb-like 2PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 210210N-terminal region (NRD)Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi359 – 36810Poly-Lys

Domaini

The N-terminal region (NRD) inhibits DNA-binding via its interaction with the C-terminal region.

Sequence similaritiesi

Contains 2 Myb-like domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG262979.
GeneTreeiENSGT00530000063659.
HOGENOMiHOG000231811.
HOVERGENiHBG084694.
InParanoidiQ62187.
KOiK15225.
OMAiTLESEWP.
OrthoDBiEOG7ZKS9N.
PhylomeDBiQ62187.
TreeFamiTF333537.

Family and domain databases

Gene3Di1.10.10.60. 2 hits.
InterProiIPR009057. Homeodomain-like.
IPR017877. Myb-like_dom.
IPR001005. SANT/Myb.
[Graphical view]
SMARTiSM00717. SANT. 2 hits.
[Graphical view]
PROSITEiPS50090. MYB_LIKE. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q62187-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKGGTSKFKT HTETLYKKKK WSSVSEKRPQ KCPSQCLESK QPQVSVLGKR
60 70 80 90 100
RRASQTPAQE TLESEWPQKA KRKKRRREPQ TPAQETLESE WPQKAKKKKR
110 120 130 140 150
RGEPQTPTQE SLESEQPPVS LLGKRRRESQ TPAQENSESE QPRKAKRRRK
160 170 180 190 200
KRKGSQQPTS SLLKTPETFL KAKKTTSAHK KKKNSVLEVD METGIILVDK
210 220 230 240 250
ENMENLLETS RKDVDIVYVD MSKGQRSAKV RETGELPAAK PQEHGCRELL
260 270 280 290 300
GDVRSRKKQK HLQKVAPWDV VQGSQPESIS LPPSEPLSSE DLEGKSTEAA
310 320 330 340 350
VFCKKKSKKN VFRSQELEPI PDSLDDSETI SERLDSTHHG GAVGAGEECE
360 370 380 390 400
STKESHSIKK KSKKKKHKSV ALATSSDSAS VTDSKAKNAL VDSSEGSGAV
410 420 430 440 450
REEDVDHRPA EAEAQACSTE KHREAMQRLE PTHEEESNSE SASNSAARHI
460 470 480 490 500
SEDRRESDDS DVDLGSAVRQ LREFIPDIQE RAATTIRRMY RDDLGRFKEF
510 520 530 540 550
KAQGVAIRFG KFSAKENKQI EKNVQDFLSL TGIESADKLL YTDRYPEEKT
560 570 580 590 600
LITNLKRKHA FRLHIGKGIA RPWKLVYYRA KKIFDVNNYK GRYNEEDTKK
610 620 630 640 650
LKAYHSLHGN DWKKIGAMVA RSSLSVALKF SQIGGTRNQG AWSKAETQRL
660 670 680 690 700
IKAVEDVILK KMSPQELREL DSKLQEDPEG RLSIVREKLY KGISWVEVEA
710 720 730 740 750
RVETRNWMQC KSKWTEILTK RMTHGGFVYR GVNALQAKIT LIERLYELNV
760 770 780 790 800
NDANEIDWED LASAIGDVPP PFVQAKFYKL KAACVPFWQK KTFPEIIDYL
810 820 830 840 850
YKNSLPLLKE KLDKKMKKKD GQIQTPAAPK QDFLFKDIFH CDDDSDEGSP

EEPSASDVQ
Length:859
Mass (Da):97,723
Last modified:October 3, 2006 - v2
Checksum:iD47950ECB3A08DCD
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti72 – 9625Missing in CAA58808. (PubMed:7720715)CuratedAdd
BLAST
Sequence conflicti305 – 3073KKS → RSL in CAA58808. (PubMed:7720715)Curated
Sequence conflicti348 – 3481Missing in CAA58808. (PubMed:7720715)Curated
Sequence conflicti496 – 4961R → L AA sequence (PubMed:7720715)Curated
Sequence conflicti762 – 7621A → C in CAA58808. (PubMed:7720715)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X83974 mRNA. Translation: CAA58808.1.
AF237703 mRNA. Translation: AAF43448.1.
BC059011 mRNA. Translation: AAH59011.1.
CCDSiCCDS38089.1.
PIRiS54776.
RefSeqiNP_033468.2. NM_009442.2.
XP_006497915.1. XM_006497852.1.
UniGeneiMm.252195.

Genome annotation databases

EnsembliENSMUST00000100237; ENSMUSP00000097809; ENSMUSG00000026803.
GeneIDi22130.
KEGGimmu:22130.
UCSCiuc008izk.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X83974 mRNA. Translation: CAA58808.1.
AF237703 mRNA. Translation: AAF43448.1.
BC059011 mRNA. Translation: AAH59011.1.
CCDSiCCDS38089.1.
PIRiS54776.
RefSeqiNP_033468.2. NM_009442.2.
XP_006497915.1. XM_006497852.1.
UniGeneiMm.252195.

3D structure databases

ProteinModelPortaliQ62187.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204361. 2 interactions.

Proteomic databases

MaxQBiQ62187.
PaxDbiQ62187.
PRIDEiQ62187.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000100237; ENSMUSP00000097809; ENSMUSG00000026803.
GeneIDi22130.
KEGGimmu:22130.
UCSCiuc008izk.1. mouse.

Organism-specific databases

CTDi7270.
MGIiMGI:105044. Ttf1.

Phylogenomic databases

eggNOGiNOG262979.
GeneTreeiENSGT00530000063659.
HOGENOMiHOG000231811.
HOVERGENiHBG084694.
InParanoidiQ62187.
KOiK15225.
OMAiTLESEWP.
OrthoDBiEOG7ZKS9N.
PhylomeDBiQ62187.
TreeFamiTF333537.

Enzyme and pathway databases

ReactomeiREACT_200667. NoRC negatively regulates rRNA expression.
REACT_214440. NoRC negatively regulates rRNA expression.
REACT_235780. RNA Polymerase I Transcription Initiation.
REACT_257165. RNA Polymerase I Transcription Termination.

Miscellaneous databases

NextBioi302001.
PROiQ62187.
SOURCEiSearch...

Gene expression databases

BgeeiQ62187.
CleanExiMM_TTF1.
GenevestigatoriQ62187.

Family and domain databases

Gene3Di1.10.10.60. 2 hits.
InterProiIPR009057. Homeodomain-like.
IPR017877. Myb-like_dom.
IPR001005. SANT/Myb.
[Graphical view]
SMARTiSM00717. SANT. 2 hits.
[Graphical view]
PROSITEiPS50090. MYB_LIKE. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Different domains of the murine RNA polymerase I-specific termination factor mTTF-I serve distinct functions in transcription termination."
    Evers R., Smid A., Rudloff U., Lottspeich F., Gummt I.
    EMBO J. 14:1248-1256(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 394-400 AND 457-498, FUNCTION, SUBCELLULAR LOCATION.
    Tissue: Ehrlich ascites tumor cell.
  2. Hu Q., Rothblum L.
    Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain.
  4. "Oligomerization of the transcription termination factor TTF-I: implications for the structural organization of ribosomal transcription units."
    Sander E.E., Grummt I.
    Nucleic Acids Res. 25:1142-1147(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT.
  5. "Termination of mammalian rDNA replication: polar arrest of replication fork movement by transcription termination factor TTF-I."
    Gerber J.-K., Goegel E., Berger C., Wallisch M., Mueller F., Grummt I., Grummt F.
    Cell 90:559-567(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "RNA polymerase I transcription on nucleosomal templates: the transcription termination factor TTF-I induces chromatin remodeling and relieves transcriptional repression."
    Laengst G., Blank T.A., Becker P.B., Grummt I.
    EMBO J. 16:760-768(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "The chromatin remodeling complex NoRC and TTF-I cooperate in the regulation of the mammalian rRNA genes in vivo."
    Nemeth A., Strohner R., Grummt I., Laengst G.
    Nucleic Acids Res. 32:4091-4099(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH BAZ2A, FUNCTION.
  8. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-457 AND SER-460, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiTTF1_MOUSE
AccessioniPrimary (citable) accession number: Q62187
Secondary accession number(s): Q9JKK5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 3, 2006
Last sequence update: October 3, 2006
Last modified: January 7, 2015
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.