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Protein

RalA-binding protein 1

Gene

Ralbp1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Can activate specifically hydrolysis of GTP bound to RAC1 and CDC42, but not RALA. Mediates ATP-dependent transport of S-(2,4-dinitrophenyl)-glutathione (DNP-SG) and doxorubicin (DOX) and is the major ATP-dependent transporter of glutathione conjugates of electrophiles (GS-E) and DOX in erythrocytes. Can catalyze transport of glutathione conjugates and xenobiotics, and may contribute to the multidrug resistance phenomenon. Serves as a scaffold protein that brings together proteins forming an endocytotic complex during interphase and also with CDK1 to switch off endocytosis, One of its substrates would be EPN1/Epsin (By similarity).By similarity

GO - Molecular functioni

  • ATPase activity Source: MGI
  • GTPase activator activity Source: UniProtKB
  • Rac GTPase binding Source: MGI
  • Ral GTPase binding Source: UniProtKB
  • transmembrane transporter activity Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Keywords - Biological processi

Transport

Enzyme and pathway databases

ReactomeiR-MMU-194840. Rho GTPase cycle.

Names & Taxonomyi

Protein namesi
Recommended name:
RalA-binding protein 1
Short name:
RalBP1
Alternative name(s):
Dinitrophenyl S-glutathione ATPase
Short name:
DNP-SG ATPase
Ral-interacting protein 1
Gene namesi
Name:Ralbp1
Synonyms:Rip1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:108466. Ralbp1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 648647RalA-binding protein 1PRO_0000056734Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylthreonineBy similarity
Modified residuei29 – 291PhosphoserineCombined sources
Modified residuei30 – 301PhosphoserineCombined sources
Modified residuei34 – 341PhosphoserineBy similarity
Modified residuei44 – 441PhosphothreonineBy similarity
Modified residuei48 – 481PhosphoserineCombined sources
Modified residuei62 – 621PhosphoserineBy similarity
Modified residuei92 – 921PhosphoserineCombined sources
Modified residuei93 – 931PhosphoserineCombined sources
Modified residuei461 – 4611PhosphoserineBy similarity
Modified residuei463 – 4631PhosphoserineBy similarity
Modified residuei638 – 6381PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ62172.
MaxQBiQ62172.
PaxDbiQ62172.
PRIDEiQ62172.

PTM databases

iPTMnetiQ62172.
PhosphoSiteiQ62172.

Expressioni

Tissue specificityi

Ubiquitous. The highest level of expression was observed in ovaries and skeletal muscle, whereas the lowest was found in spleen, liver and peripheral blood leukocytes.

Gene expression databases

BgeeiENSMUSG00000024096.
CleanExiMM_RALBP1.
GenevisibleiQ62172. MM.

Interactioni

Subunit structurei

Interacts with the GTP-bound form of RALA, RALB, CDC42 and RAC1. Interacts with REPS1 and REPS2 and this does not affect the Ral-binding activity. Interacts with DAB2IP. Interacts with catalytically active CCNB1 and CDK1 during mitosis. Interacts with EPN1, NUMB and TFAP2A during interphase and mitosis (By similarity).By similarity

GO - Molecular functioni

  • Rac GTPase binding Source: MGI
  • Ral GTPase binding Source: UniProtKB

Protein-protein interaction databases

DIPiDIP-29741N.
IntActiQ62172. 3 interactions.
MINTiMINT-4132030.
STRINGi10090.ENSMUSP00000024905.

Structurei

3D structure databases

ProteinModelPortaliQ62172.
SMRiQ62172. Positions 184-446.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini192 – 380189Rho-GAPPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni391 – 44656Interacts with RalAAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi164 – 1718Poly-Lys

Sequence similaritiesi

Contains 1 Rho-GAP domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG4370. Eukaryota.
ENOG410XRJ9. LUCA.
GeneTreeiENSGT00840000129752.
HOGENOMiHOG000007929.
HOVERGENiHBG044496.
InParanoidiQ62172.
KOiK08773.
OMAiYDELMEW.
OrthoDBiEOG091G01CT.
TreeFamiTF315411.

Family and domain databases

Gene3Di1.10.555.10. 1 hit.
InterProiIPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
[Graphical view]
PfamiPF00620. RhoGAP. 1 hit.
[Graphical view]
SMARTiSM00324. RhoGAP. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
PROSITEiPS50238. RHOGAP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q62172-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTECFLPPSS SPSEHRRAEH GSGLTRTPSS EEISPTKFPG LYRTGEPSPP
60 70 80 90 100
HDVLHEPPDT VSDDDKDHGK KKGKFKKKEK RTEGYAAFQE DSSGDEAESP
110 120 130 140 150
SKVKRSKGIH VFKKPSFSKK KEKDFKIKEK PKEEKHKEEK HKEEKHKEKK
160 170 180 190 200
SKDLTAADVV KQWKEKKKKK KPIQEPEVPQ MDAPSVKPIF GVPLVDAVER
210 220 230 240 250
TMMYDGVRLP AVFRECVDYM EKHGMKCEGV YRVSGIKSKV DELKAAYDRE
260 270 280 290 300
ESPNLEEYEP NTVASLLKQY LRDLPENLLT KELMPRFEEA CGKTTEMEKV
310 320 330 340 350
QEFQRLLREL PECNHLLLSW LIVHLDHVIA KELETKMNIQ NISIVLSPTV
360 370 380 390 400
QISNRVLYVL FTHVQELFGT VVLKQVTRPL RWSNMATMPT LPETQAGIKE
410 420 430 440 450
EIRRQEFLLN CLHRDLQGGI KDLSKEERLW EVQRILTALK RKLREAKRQE
460 470 480 490 500
CETKIAQEIA SLSKEDVSKE EMNENEEVIN ILLAQENEIL TEQEELLAME
510 520 530 540 550
QFLRRQIASE KEEIDRLRAE IAEIQSRQQH GRSETEEYSS DSESESEDEE
560 570 580 590 600
ELQLILEDLQ RQNEELEIKN NHLNQAVHEE REAIIELRVQ LRLLQMQRAK
610 620 630 640
SEQQPQEEEE PERRGGIGPP PCDGVLEVRV AKEQAKASPS KDRKETPI
Length:648
Mass (Da):75,043
Last modified:July 27, 2011 - v4
Checksum:i4C833CCA4E6FDBA6
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti30 – 301S → F in BAB31552 (PubMed:16141072).Curated
Sequence conflicti617 – 6171I → T in CAA56912 (PubMed:8570186).Curated
Sequence conflicti617 – 6171I → T in AAH75732 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X80937 mRNA. Translation: CAA56912.1.
BC075732 mRNA. Translation: AAH75732.1.
BC067073 mRNA. Translation: AAH67073.1.
BC076636 mRNA. Translation: AAH76636.1.
AK019116 mRNA. Translation: BAB31552.1.
CCDSiCCDS37678.1.
RefSeqiNP_001185878.1. NM_001198949.1.
NP_033093.2. NM_009067.5.
XP_006523977.1. XM_006523914.2.
UniGeneiMm.17009.
Mm.486223.

Genome annotation databases

EnsembliENSMUST00000024905; ENSMUSP00000024905; ENSMUSG00000024096.
ENSMUST00000166543; ENSMUSP00000129448; ENSMUSG00000024096.
GeneIDi19765.
KEGGimmu:19765.
UCSCiuc008dgn.3. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X80937 mRNA. Translation: CAA56912.1.
BC075732 mRNA. Translation: AAH75732.1.
BC067073 mRNA. Translation: AAH67073.1.
BC076636 mRNA. Translation: AAH76636.1.
AK019116 mRNA. Translation: BAB31552.1.
CCDSiCCDS37678.1.
RefSeqiNP_001185878.1. NM_001198949.1.
NP_033093.2. NM_009067.5.
XP_006523977.1. XM_006523914.2.
UniGeneiMm.17009.
Mm.486223.

3D structure databases

ProteinModelPortaliQ62172.
SMRiQ62172. Positions 184-446.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-29741N.
IntActiQ62172. 3 interactions.
MINTiMINT-4132030.
STRINGi10090.ENSMUSP00000024905.

PTM databases

iPTMnetiQ62172.
PhosphoSiteiQ62172.

Proteomic databases

EPDiQ62172.
MaxQBiQ62172.
PaxDbiQ62172.
PRIDEiQ62172.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000024905; ENSMUSP00000024905; ENSMUSG00000024096.
ENSMUST00000166543; ENSMUSP00000129448; ENSMUSG00000024096.
GeneIDi19765.
KEGGimmu:19765.
UCSCiuc008dgn.3. mouse.

Organism-specific databases

CTDi10928.
MGIiMGI:108466. Ralbp1.

Phylogenomic databases

eggNOGiKOG4370. Eukaryota.
ENOG410XRJ9. LUCA.
GeneTreeiENSGT00840000129752.
HOGENOMiHOG000007929.
HOVERGENiHBG044496.
InParanoidiQ62172.
KOiK08773.
OMAiYDELMEW.
OrthoDBiEOG091G01CT.
TreeFamiTF315411.

Enzyme and pathway databases

ReactomeiR-MMU-194840. Rho GTPase cycle.

Miscellaneous databases

ChiTaRSiRalbp1. mouse.
PROiQ62172.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000024096.
CleanExiMM_RALBP1.
GenevisibleiQ62172. MM.

Family and domain databases

Gene3Di1.10.555.10. 1 hit.
InterProiIPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
[Graphical view]
PfamiPF00620. RhoGAP. 1 hit.
[Graphical view]
SMARTiSM00324. RhoGAP. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
PROSITEiPS50238. RHOGAP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRBP1_MOUSE
AccessioniPrimary (citable) accession number: Q62172
Secondary accession number(s): Q6NXH5, Q9CRE5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 15, 2003
Last sequence update: July 27, 2011
Last modified: September 7, 2016
This is version 133 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.