Q62170 (SELPL_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
P-selectin glycoprotein ligand 1
Short name=PSGL-1

Alternative name(s):
Selectin P ligand
CD_antigen=CD162

Gene names

Name:	Selplg
Synonyms:	Selp1, Selpl

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	397 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	A SLe(x)-type glycan, which through high affinity, calcium-dependent interactions with E- and P-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. PSGL1 is critical for the initial leukocyte capture. Ref.3 Ref.4 Ref.6
Subunit structure	Homodimer; disulfide-linked. Interacts with P- and E-selectins, through their lectin/EGF domains. Interaction with P-selectin requires sialyl Lewis X glycan modification and tyrosine sulfation, probably on Tyr-54, for high affinity binding By similarity. Dimerization appears not to be required for P-selectin/SELP binding By similarity. Interacts with SNX20 By similarity. Interacts with MSN and SYK; mediates SYK activation downstream of SELPLG By similarity. Ref.1 Ref.3 Ref.5
Subcellular location	Cell membrane; Single-pass membrane protein Potential.
Tissue specificity	Highly expressed in blood, bone marrow, brain, adipose tissue, spleen, and thymus. Also expressed in heart, kidney, liver, muscle, ovary, and stomach. Ref.1
Post-translational modification	Displays complex, core-2, sialylated and fucosylated O-linked oligosaccharides, at least some of which appear to contain poly-N-acetyllactosamine with varying degrees of substitution. Mainly disialylated or neutral forms of the core-2 tetrasaccharide, Galbeta1-->4GlcNAcbeta1-->6(Galbeta1-->3)GalNAcOH. The GlcN:GalN ratio is approximately 2:1 and the Man:Fuc ratio 3:5. Contains about 14% fucose with alpha-1,3 linkage present in two forms: One species is a disialylated, monofucosylated glycan, and the other, a monosialylated, trifucosylated glycan with a polylactosamine backbone. The fucosylated forms carry the Lewis antigen and are important for interaction with selectins and for functioning. No sulfated O-glycans. Some N-glycosylation By similarity.

Ontologies

Keywords
Biological process	Cell adhesion
Cellular component	Cell membrane Membrane
Domain	Repeat Signal Transmembrane Transmembrane helix
Ligand	Sialic acid
PTM	Cleavage on pair of basic residues Disulfide bond Glycoprotein Pyrrolidone carboxylic acid Sulfation
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular response to interleukin-6 Inferred from electronic annotation. Source: Compara leukocyte adhesive activation Inferred from mutant phenotype PubMed 17632516. Source: UniProtKB leukocyte tethering or rolling Inferred from mutant phenotype PubMed 15956287. Source: MGI
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 17

17

Potential

Propeptide

18 – 41

24

By similarity

PRO_0000022304

Chain

42 – 397

356

P-selectin glycoprotein ligand 1

PRO_0000022305

Regions

Topological domain

18 – 307

290

Extracellular Potential

Transmembrane

308 – 328

21

Helical; Potential

Topological domain

329 – 397

69

Cytoplasmic Potential

Repeat

126 – 135

10

1

Repeat

136 – 145

10

2

Repeat

146 – 155

10

3

Repeat

156 – 165

10

4

Repeat

166 – 175

10

5

Repeat

176 – 185

10

6

Repeat

186 – 195

10

7

Repeat

196 – 205

10

8

Repeat

206 – 215

10

9

Repeat

216 – 225

10

10

Region

126 – 225

100

10 X 10 AA tandem repeats

Amino acid modifications

Modified residue

42

1

Pyrrolidone carboxylic acid By similarity

Modified residue

54

1

Sulfotyrosine Probable

Modified residue

56

1

Sulfotyrosine Potential

Glycosylation

58

1

O-linked (GalNAc...) Probable

Glycosylation

66

1

N-linked (GlcNAc...) Potential

Glycosylation

261

1

N-linked (GlcNAc...) Potential

Disulfide bond

307

Interchain By similarity

Experimental info

Mutagenesis

54

1

Y → F: Greatly decreased P-selectin binding and tethering and rolling of cells. No further reduction of P-selectin binding; when associated with Y-56. Binding of P-selectin completely abolished; when associated with A-55; Y-56 and A-58. Ref.5

Mutagenesis

55

1

T → A: No effect on P-selectin binding. Greatly reduced P-selectin binding and tethering and rolling of cells; when associated with A-58. Binding of P-selectin completely abolished; when associated with Y-54; Y-56 and A-58. Ref.5

Mutagenesis

56

1

Y → F: No effect on P-selectin binding. Greatly decreased P-selectin binding and tethering and rolling of cells; when associated with Y-54. Binding of P-selectin completely abolished; when associated with Y-54; A-55 and A-58. Ref.5

Mutagenesis

58

1

T → A: Greatly decreased P-selectin binding and tethering and rolling of cells. No further reduction in P-selectin binding when associated with A-55. Binding of P-selectin completely abolished; when associated with Y-54; A-55; and Y-56. Ref.5

Mutagenesis

66

1

N → T: No effect on P-selectin binding; when associated with A-261.

Mutagenesis

261

1

N → A: No effect on P-selectin binding; when associated with T-66.

Sequence conflict

173

1

E → D in CAA62583. Ref.1

Sequence conflict

176

1

Q → K in CAA62583. Ref.1

Sequence conflict

180

1

M → T in CAA62583. Ref.1

Sequence conflict

183

1

D → E in CAA62583. Ref.1

Sequence conflict

186

1

Q → K in CAA62583. Ref.1

Sequence conflict

190

1

M → T in CAA62583. Ref.1

Secondary structure

1

.

.

.

397

Helix Strand Turn

Details...

Beta strand

337 – 342

6

Sequences

	Sequence		Length	Mass (Da)
	Q62170 [UniParc]. Last modified July 27, 2011. Version 2. Checksum: D5EB53D493AE26EE	FASTA	397	41,842
	10 20 30 40 50 60 MSPSFLVLLT ILGPGNSLQL QDPWGHETKE APGPVHLRER RQVVGDDDFE DPDYTYNTDP 70 80 90 100 110 120 PELLKNVTNT VAAHPELPTT VVMLERDSTS AGTSERATEK IATTDPTAPG TGGTAVGMLS 130 140 150 160 170 180 TDSATQWSLT SVETVQPAST EVETSQPAPM EAETSQPAPM EAETSQPAPM EAETSQPAPM 190 200 210 220 230 240 EADTSQPAPM EAETSQPAPN EAETSKPAPT EAETSKPAPT EAETTQLPRI QAVKTLFTTS 250 260 270 280 290 300 AATEVPSTEP TTMETASTES NESTIFLGPS VTHLPDSGLK KGLIVTPGNS PAPTLPGSSD 310 320 330 340 350 360 LIPVKQCLLI ILILASLATI FLVCTVVLAV RLSRKTHMYP VRNYSPTEMI CISSLLPEGG 370 380 390 DGAPVTANGG LPKVQDLKTE PSGDRDGDDL TLHSFLP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mouse P-selectin glycoprotein ligand-1: molecular cloning, chromosomal localization, and expression of a functional P-selectin receptor." Yang J., Galipeau J., Kozak C., Furie B.C., Furie B. Blood 87:4176-4186(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], INTERACTION WITH SELE AND SELP, TISSUE SPECIFICITY. Strain: BALB/c.
[2]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[3]	"P-Selectin glycoprotein ligand 1 (PSGL-1) is a physiological ligand for E-selectin in mediating T helper 1 lymphocyte migration." Hirata T., Merrill-Skoloff G., Aab M., Yang J., Furie B.C., Furie B. J. Exp. Med. 192:1669-1676(2000) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH SELE AND SELP, FUNCTION.
[4]	"P-, E-, and L-selectin mediate migration of activated CD8+ T lymphocytes into inflamed skin." Hirata T., Furie B.C., Furie B. J. Immunol. 169:4307-4313(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[5]	"N-terminal residues in murine P-selectin glycoprotein ligand-1 required for binding to murine P-selectin." Xia L., Ramachandran V., McDaniel J.M., Nguyen K.N., Cummings R.D., McEver R.P. Blood 101:552-559(2003) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH SELP, SULFATION, MUTAGENESIS OF TYR-54; THR-55; TYR-56 AND THR-58.
[6]	"Complete identification of E-selectin ligands on neutrophils reveals distinct functions of PSGL-1, ESL-1, and CD44." Hidalgo A., Peired A.J., Wild M.K., Vestweber D., Frenette P.S. Immunity 26:477-489(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X91144 mRNA. Translation: CAA62583.1.
AC159240 Genomic DNA. No translation available.

IPI

IPI00311080.

UniGene

Mm.332590.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2EMT	X-ray	2.80	C/D/E	331-348	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-59330N.

STRING

10090.ENSMUSP00000098436.

PTM databases

PhosphoSite

Q62170.

Proteomic databases

PaxDb

Q62170.

PRIDE

Q62170.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Organism-specific databases

MGI

MGI:106689. Selplg.

Phylogenomic databases

eggNOG

NOG300903.

HOGENOM

HOG000013048.

HOVERGEN

HBG061628.

Gene expression databases

ArrayExpress

Q62170.

Bgee

Q62170.

CleanEx

MM_SELPLG.

Genevestigator

Q62170.

GermOnline

ENSMUSG00000048163. Mus musculus.

Family and domain databases

InterPro

IPR026195. PSGL-1.
[Graphical view]

PANTHER

PTHR17384. PTHR17384. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

Q62170.

SOURCE

Search...

Entry information

Entry name

SELPL_MOUSE

Accession

Primary (citable) accession number: Q62170

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	July 27, 2011
Last modified:	May 1, 2013
This is version 94 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program