Q62159 (RHOC_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Rho-related GTP-binding protein RhoC

Alternative name(s):
Silica-induced gene 61 protein
Short name=SIG-61

Gene names

Name:	Rhoc
Synonyms:	Arhc

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	193 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Serves as a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Regulates apical junction formation in bronchial epithelial cells. Ref.6
Subunit structure	Interacts with AKAP13, DIAPH1, ROCK1 and ROCK2 By similarity. Interacts with PKN2 and RTKN. Ref.4 Ref.6
Subcellular location	Cell membrane; Lipid-anchor; Cytoplasmic side Potential. Cleavage furrow By similarity. Note: Translocates to the equatorial region before furrow formation in a ECT2-dependent manner By similarity.
Induction	Up-regulated in silica-treated macrophages. Ref.1
Sequence similarities	Belongs to the small GTPase superfamily. Rho family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Ligand	GTP-binding Nucleotide-binding
PTM	Lipoprotein Methylation Phosphoprotein Prenylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	apical junction assembly Inferred from direct assay Ref.6. Source: UniProtKB cell cycle cytokinesis Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of I-kappaB kinase/NF-kappaB cascade Inferred from electronic annotation. Source: Compara small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro
Cellular_component	cleavage furrow Inferred from sequence or structural similarity. Source: UniProtKB cytosol Inferred from electronic annotation. Source: Compara nucleus Inferred from direct assay PubMed 15093731. Source: MGI plasma membrane Inferred from direct assay PubMed 15093731. Source: MGI
Molecular_function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW signal transducer activity Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 190	190	Rho-related GTP-binding protein RhoC		PRO_0000042024
	Propeptide	191 – 193	3	Removed in mature form By similarity		PRO_0000042025
Regions
	Nucleotide binding	12 – 19	8	GTP By similarity
	Nucleotide binding	59 – 63	5	GTP By similarity
	Nucleotide binding	117 – 120	4	GTP By similarity
	Motif	34 – 42	9	Effector region Potential
Amino acid modifications
	Modified residue	19	1	Phosphothreonine Ref.5
	Modified residue	26	1	Phosphoserine Ref.5
	Modified residue	190	1	Cysteine methyl ester By similarity
	Lipidation	190	1	S-geranylgeranyl cysteine By similarity
Experimental info
	Sequence conflict	102	1	E → Q in CAA56682. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q62159 [UniParc]. Last modified April 13, 2004. Version 2. Checksum: A193DA581560F131	FASTA	193	22,006
	10 20 30 40 50 60 MAAIRKKLVI VGDGACGKTC LLIVFSKDQF PEVYVPTVFE NYIADIEVDG KQVELALWDT 70 80 90 100 110 120 AGQEDYDRLR PLSYPDTDVI LMCFSIDSPD SLENIPEKWT PEVKHFCPNV PIILVGNKKD 130 140 150 160 170 180 LRQDEHTRRE LAKMKQEPVR SEEGRDMANR ISAFGYLECS AKTKEGVREV FEMATRAGLQ 190 VRKNKRRRGC PIL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation of nine gene sequences induced by silica in murine macrophages." Segade F., Claudio E., Wrobel K., Ramos S., Lazo P.S. J. Immunol. 154:2384-2392(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION. Tissue: Macrophage.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Bone marrow.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Mammary tumor.
[4]	"Rhotekin, a new putative target for Rho bearing homology to a serine/threonine kinase, PKN, and rhophilin in the rho-binding domain." Reid T., Furuyashiki T., Ishizaki T., Watanabe G., Watanabe N., Fujisawa K., Morii N., Madaule P., Narumiya S. J. Biol. Chem. 271:13556-13560(1996) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH RTKN.
[5]	"Protein phosphorylation and expression profiling by Yin-yang multidimensional liquid chromatography (Yin-yang MDLC) mass spectrometry." Dai J., Jin W.-H., Sheng Q.-H., Shieh C.-H., Wu J.-R., Zeng R. J. Proteome Res. 6:250-262(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-19 AND SER-26, MASS SPECTROMETRY. Tissue: Liver.
[6]	"The Rho target PRK2 regulates apical junction formation in human bronchial epithelial cells." Wallace S.W., Magalhaes A., Hall A. Mol. Cell. Biol. 31:81-91(2011) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH PKN2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X80638 mRNA. Translation: CAA56682.1. AK011599 mRNA. Translation: BAB27724.1. AK152802 mRNA. Translation: BAE31507.1. AK160650 mRNA. Translation: BAE35941.1. BC004627 mRNA. Translation: AAH04627.1.
IPI	IPI00122245.
RefSeq	NP_031510.2. NM_007484.2.
UniGene	Mm.262.
3D structure databases
ProteinModelPortal	Q62159.
ModBase	Search...
PTM databases
PhosphoSite	Q62159.
Proteomic databases
PaxDb	Q62159.
PRIDE	Q62159.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000002303; ENSMUSP00000002303; ENSMUSG00000002233. ENSMUST00000106787; ENSMUSP00000102399; ENSMUSG00000002233.
GeneID	11853.
KEGG	mmu:11853.
UCSC	uc009rpe.2. mouse.
Organism-specific databases
CTD	389.
MGI	MGI:106028. Rhoc.
Phylogenomic databases
eggNOG	COG1100.
GeneTree	ENSGT00700000104143.
HOGENOM	HOG000233974.
HOVERGEN	HBG009351.
InParanoid	Q62159.
KO	K07857.
OMA	NKKRRGC.
OrthoDB	EOG4G4GRD.
Gene expression databases
ArrayExpress	Q62159.
Bgee	Q62159.
CleanEx	MM_RHOC.
Genevestigator	Q62159.
GermOnline	ENSMUSG00000002233. Mus musculus.
Family and domain databases
InterPro	IPR005225. Small_GTP-bd_dom. IPR001806. Small_GTPase. IPR003578. Small_GTPase_Rho. [Graphical view]
Pfam	PF00071. Ras. 1 hit. [Graphical view]
PRINTS	PR00449. RASTRNSFRMNG.
SMART	SM00174. RHO. 1 hit. [Graphical view]
TIGRFAMs	TIGR00231. small_GTP. 1 hit.
PROSITE	PS51420. RHO. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	279829.
SOURCE	Search...

Entry information

Entry name

RHOC_MOUSE

Accession

Primary (citable) accession number: Q62159
Secondary accession number(s): Q3TUN0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	April 13, 2004
Last modified:	May 1, 2013
This is version 123 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program