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Protein

Zinc finger protein RFP

Gene

Trim27

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase that mediates ubiquitination of PIK3C2B and inhibits its activity; mediates the formation of 'Lys-48'-linked polyubiquitin chains; the function inhibits CD4 T-cell activation. Acts as a regulator of retrograde transport: together with MAGEL2, mediates the formation of 'Lys-63'-linked polyubiquitin chains at 'Lys-220' of WASHC1, leading to promote endosomal F-actin assembly. Has a transcriptional repressor activity. Induces apoptosis by activating Jun N-terminal kinase and p38 kinase and also increases caspase-3-like activity independently of mitochondrial events. May function in male germ cell development. Has DNA-binding activity and preferentially bound to double-stranded DNA.By similarity

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri16 – 57RING-typePROSITE-ProRule annotationAdd BLAST42
Zinc fingeri91 – 132B box-typePROSITE-ProRule annotationAdd BLAST42

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Repressor, Transferase
Biological processTranscription, Transcription regulation, Transport, Ubl conjugation pathway
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein RFP (EC:2.3.2.27)
Alternative name(s):
RING-type E3 ubiquitin transferase TRIM27Curated
Ret finger protein
Tripartite motif-containing protein 27
Gene namesi
Name:Trim27
Synonyms:Rfp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:97904. Trim27.

Subcellular locationi

  • Nucleus By similarity
  • Cytoplasm By similarity
  • NucleusPML body By similarity
  • Endosome By similarity

  • Note: Nuclear or cytoplasmic depending on the cell type. Recruited to retromer-containing endosomes via interaction with MAGEL2 (By similarity). Colocalized with PML and EIF3S6 in nuclear bodies.By similarity1 Publication

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm, Endosome, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000562411 – 513Zinc finger protein RFPAdd BLAST513

Proteomic databases

EPDiQ62158.
MaxQBiQ62158.
PaxDbiQ62158.
PeptideAtlasiQ62158.
PRIDEiQ62158.

PTM databases

iPTMnetiQ62158.
PhosphoSitePlusiQ62158.

Expressioni

Tissue specificityi

Expressed strongly in testis. Low levels were detected in spleen, thymus, cerebrum and cerebellum.2 Publications

Gene expression databases

BgeeiENSMUSG00000021326.
CleanExiMM_TRIM27.
ExpressionAtlasiQ62158. baseline and differential.
GenevisibleiQ62158. MM.

Interactioni

Subunit structurei

Homomultimerizes. Interacts with PML, EIF3S6, EPC1, CHD4 and EID1. Interacts with MAGED4, MAGEF1 and MAGEL2.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Plcg2Q8CIH54EBI-642025,EBI-617954

GO - Molecular functioni

Protein-protein interaction databases

BioGridi202871. 7 interactors.
IntActiQ62158. 2 interactors.
MINTiMINT-1591463.
STRINGi10090.ENSMUSP00000021761.

Structurei

3D structure databases

ProteinModelPortaliQ62158.
SMRiQ62158.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini298 – 492B30.2/SPRYPROSITE-ProRule annotationAdd BLAST195

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili132 – 171Sequence analysisAdd BLAST40
Coiled coili282 – 311Sequence analysisAdd BLAST30

Domaini

The coiled-coil region mediates interaction with EPC1 and CHD4. The B box and coiled-coil domains mediate interaction with PML. The B box and the distal coiled-coil domains mediate homomultimerisation. The B30.2 domain mediates interaction with EIF3S6.

Sequence similaritiesi

Belongs to the TRIM/RBCC family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri16 – 57RING-typePROSITE-ProRule annotationAdd BLAST42
Zinc fingeri91 – 132B box-typePROSITE-ProRule annotationAdd BLAST42

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiENOG410KDQE. Eukaryota.
ENOG410Z5PW. LUCA.
GeneTreeiENSGT00760000118893.
HOVERGENiHBG001357.
InParanoidiQ62158.
KOiK12009.
OMAiHAHSMET.
OrthoDBiEOG091G05W2.
PhylomeDBiQ62158.
TreeFamiTF350411.

Family and domain databases

CDDicd00162. RING. 1 hit.
Gene3Di3.30.40.10. 1 hit.
InterProiView protein in InterPro
IPR001870. B30.2/SPRY.
IPR003879. Butyrophylin.
IPR013320. ConA-like_dom.
IPR006574. PRY.
IPR003877. SPRY_dom.
IPR000315. Znf_B-box.
IPR020457. Znf_B-box_chordata.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
PfamiView protein in Pfam
PF13765. PRY. 1 hit.
PF00622. SPRY. 1 hit.
PF00643. zf-B_box. 1 hit.
PRINTSiPR01406. BBOXZNFINGER.
PR01407. BUTYPHLNCDUF.
SMARTiView protein in SMART
SM00336. BBOX. 1 hit.
SM00589. PRY. 1 hit.
SM00184. RING. 1 hit.
SM00449. SPRY. 1 hit.
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiView protein in PROSITE
PS50188. B302_SPRY. 1 hit.
PS50119. ZF_BBOX. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q62158-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASGSVAECL QQETTCPVCL QYFVEPMMLD CGHNICCACL ARCWGAAETN
60 70 80 90 100
VSCPQCRETF PQRHMRPNRH LANVTQLVKQ LRTERPSGPG GEMGVCEKHR
110 120 130 140 150
EPLKLYCEQD QMPICVVCDR SREHRGHSVL PLEEAVEGFK EQIQNRLDHL
160 170 180 190 200
RRVKDLKKRR RAQGEQARAE LLSLTQMERE KIVWEFEQLY HSLKEHEYRL
210 220 230 240 250
LARLEELDLA IYNSINGAIT QFSCNISHLS GLIAQLEEKQ QQPTRELLQD
260 270 280 290 300
IGDTLSRAER IRIPEPWITP PDLQEKIHIF AQKCLFLTES LKQFTEKMQS
310 320 330 340 350
DMEKIQELRE AQLYSVDVTL DPDTAYPSLI LSDNLRQVRY SYLQQDLPDN
360 370 380 390 400
PERFNLFPCV LGSPCFIAGR HYWEVEVGDK AKWTIGVCED SVCRKGGVTS
410 420 430 440 450
APQNGFWAVS LWYGKEYWAL TSPMTALPLR TPLQRVGIFL DYDAGEVSFY
460 470 480 490 500
NVTERCHTFT FSHATFCGPV RPYFSLSYSG GKSAAPLIIC PMSGIDGFSG
510
HVGNHGHSME TSP
Length:513
Mass (Da):58,513
Last modified:July 19, 2004 - v2
Checksum:i9384B6678352A434
GO

Sequence cautioni

The sequence AAA85354 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti119 – 120DR → EP in CAA53092 (Ref. 4) Curated2
Sequence conflicti126G → D in AAA85354 (PubMed:9023983).Curated1
Sequence conflicti367I → M in BAC40192 (PubMed:16141072).Curated1
Sequence conflicti367I → M in CAA53092 (Ref. 4) Curated1
Sequence conflicti485A → R in AAA85354 (PubMed:9023983).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L46855 mRNA. Translation: AAA85354.1. Different initiation.
AK088180 mRNA. Translation: BAC40192.1.
BC003219 mRNA. Translation: AAH03219.1.
X75343 mRNA. Translation: CAA53092.1.
CCDSiCCDS36607.1.
PIRiS37583.
RefSeqiNP_033080.2. NM_009054.3.
XP_006516669.1. XM_006516606.3.
UniGeneiMm.314056.

Genome annotation databases

EnsembliENSMUST00000021761; ENSMUSP00000021761; ENSMUSG00000021326.
GeneIDi19720.
KEGGimmu:19720.
UCSCiuc007ppy.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L46855 mRNA. Translation: AAA85354.1. Different initiation.
AK088180 mRNA. Translation: BAC40192.1.
BC003219 mRNA. Translation: AAH03219.1.
X75343 mRNA. Translation: CAA53092.1.
CCDSiCCDS36607.1.
PIRiS37583.
RefSeqiNP_033080.2. NM_009054.3.
XP_006516669.1. XM_006516606.3.
UniGeneiMm.314056.

3D structure databases

ProteinModelPortaliQ62158.
SMRiQ62158.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202871. 7 interactors.
IntActiQ62158. 2 interactors.
MINTiMINT-1591463.
STRINGi10090.ENSMUSP00000021761.

PTM databases

iPTMnetiQ62158.
PhosphoSitePlusiQ62158.

Proteomic databases

EPDiQ62158.
MaxQBiQ62158.
PaxDbiQ62158.
PeptideAtlasiQ62158.
PRIDEiQ62158.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000021761; ENSMUSP00000021761; ENSMUSG00000021326.
GeneIDi19720.
KEGGimmu:19720.
UCSCiuc007ppy.2. mouse.

Organism-specific databases

CTDi5987.
MGIiMGI:97904. Trim27.

Phylogenomic databases

eggNOGiENOG410KDQE. Eukaryota.
ENOG410Z5PW. LUCA.
GeneTreeiENSGT00760000118893.
HOVERGENiHBG001357.
InParanoidiQ62158.
KOiK12009.
OMAiHAHSMET.
OrthoDBiEOG091G05W2.
PhylomeDBiQ62158.
TreeFamiTF350411.

Miscellaneous databases

ChiTaRSiTrim27. mouse.
PROiPR:Q62158.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000021326.
CleanExiMM_TRIM27.
ExpressionAtlasiQ62158. baseline and differential.
GenevisibleiQ62158. MM.

Family and domain databases

CDDicd00162. RING. 1 hit.
Gene3Di3.30.40.10. 1 hit.
InterProiView protein in InterPro
IPR001870. B30.2/SPRY.
IPR003879. Butyrophylin.
IPR013320. ConA-like_dom.
IPR006574. PRY.
IPR003877. SPRY_dom.
IPR000315. Znf_B-box.
IPR020457. Znf_B-box_chordata.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
PfamiView protein in Pfam
PF13765. PRY. 1 hit.
PF00622. SPRY. 1 hit.
PF00643. zf-B_box. 1 hit.
PRINTSiPR01406. BBOXZNFINGER.
PR01407. BUTYPHLNCDUF.
SMARTiView protein in SMART
SM00336. BBOX. 1 hit.
SM00589. PRY. 1 hit.
SM00184. RING. 1 hit.
SM00449. SPRY. 1 hit.
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiView protein in PROSITE
PS50188. B302_SPRY. 1 hit.
PS50119. ZF_BBOX. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTRI27_MOUSE
AccessioniPrimary (citable) accession number: Q62158
Secondary accession number(s): Q62157, Q8C2Q5, Q99LK1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 19, 2004
Last modified: May 10, 2017
This is version 160 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.