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Protein

Paired amphipathic helix protein Sin3b

Gene

Sin3b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a transcriptional repressor. Interacts with MXI1 to repress MYC responsive genes and antagonize MYC oncogenic activities. Interacts with MAD-MAX heterodimers by binding to MAD. The heterodimer then represses transcription by tethering SIN3B to DNA. Also forms a complex with FOXK1 which represses transcription. With FOXK1, regulates cell cycle progression probably by repressing cell cycle inhibitor genes expression (PubMed:22476904).3 Publications

GO - Molecular functioni

  • chromatin binding Source: MGI
  • RNA polymerase II transcription corepressor activity Source: GO_Central
  • transcription corepressor activity Source: UniProtKB

GO - Biological processi

  • cardiac muscle tissue development Source: UniProtKB
  • histone deacetylation Source: GO_Central
  • negative regulation of cell cycle Source: UniProtKB
  • negative regulation of transcription, DNA-templated Source: UniProtKB
  • negative regulation of transcription from RNA polymerase II promoter Source: GO_Central
  • skeletal muscle tissue development Source: UniProtKB
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-400206. Regulation of lipid metabolism by Peroxisome proliferator-activated receptor alpha (PPARalpha).

Names & Taxonomyi

Protein namesi
Recommended name:
Paired amphipathic helix protein Sin3b
Alternative name(s):
Histone deacetylase complex subunit Sin3b
Transcriptional corepressor Sin3b
Gene namesi
Name:Sin3b
Synonyms:Kiaa0700
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:107158. Sin3b.

Subcellular locationi

GO - Cellular componenti

  • autosome Source: MGI
  • cytoplasm Source: UniProtKB
  • nucleus Source: UniProtKB
  • Sin3 complex Source: GO_Central
  • X chromosome Source: MGI
  • XY body Source: UniProtKB
  • Y chromosome Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001215401 – 1098Paired amphipathic helix protein Sin3bAdd BLAST1098

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei667PhosphoserineCombined sources1
Modified residuei670PhosphoserineCombined sources1

Post-translational modificationi

Ubiquitinated by RNF220 that leads to proteasomal degradation.1 Publication

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ62141.
PaxDbiQ62141.
PeptideAtlasiQ62141.
PRIDEiQ62141.

PTM databases

iPTMnetiQ62141.
PhosphoSitePlusiQ62141.

Expressioni

Gene expression databases

BgeeiENSMUSG00000031622.
CleanExiMM_SIN3B.
ExpressionAtlasiQ62141. baseline and differential.
GenevisibleiQ62141. MM.

Interactioni

Subunit structurei

Interacts with FOXK1/MNF, MXI, MAD, NCOR1 and SAP30. Interaction with SUDS3 enhances the interaction with HDAC1 to form a complex. Interacts with CRY1, HCFC1, MAD3, MAD4, MAEL, REST, RNF220 and SETDB1. Interacts with C6orf89 (By similarity).By similarity13 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Foxk1P4212811EBI-591466,EBI-878270
MYCP011068EBI-591450,EBI-447544From a different organism.
Rnf220Q6PDX65EBI-591450,EBI-2795840
Suds3Q8BR655EBI-591450,EBI-591431

Protein-protein interaction databases

BioGridi203257. 16 interactors.
DIPiDIP-470N.
IntActiQ62141. 7 interactors.
MINTiMINT-1510576.
STRINGi10090.ENSMUSP00000004494.

Structurei

Secondary structure

11098
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi38 – 47Combined sources10
Turni48 – 50Combined sources3
Helixi52 – 66Combined sources15
Helixi72 – 82Combined sources11
Helixi83 – 85Combined sources3
Helixi87 – 96Combined sources10
Beta strandi99 – 101Combined sources3
Helixi152 – 167Combined sources16
Turni168 – 170Combined sources3
Helixi172 – 187Combined sources16
Beta strandi191 – 193Combined sources3
Helixi202 – 212Combined sources11
Turni213 – 215Combined sources3
Helixi217 – 226Combined sources10
Helixi229 – 231Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1E91NMR-A148-232[»]
1PD7NMR-A148-232[»]
2CR7NMR-A32-98[»]
2CZYNMR-A31-107[»]
2F05NMR-A148-252[»]
ProteinModelPortaliQ62141.
SMRiQ62141.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ62141.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini30 – 100PAH 1PROSITE-ProRule annotationAdd BLAST71
Domaini145 – 230PAH 2PROSITE-ProRule annotationAdd BLAST86
Domaini283 – 360PAH 3PROSITE-ProRule annotationAdd BLAST78

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 299Interaction with CRY11 PublicationAdd BLAST299
Regioni52 – 98Interaction with REST1 PublicationAdd BLAST47
Regioni275 – 499Interaction with NCOR11 PublicationAdd BLAST225
Regioni383 – 550Interaction with SUDS3 and HDAC11 PublicationAdd BLAST168

Sequence similaritiesi

Contains 3 PAH (paired amphipathic helix) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG4204. Eukaryota.
COG5602. LUCA.
GeneTreeiENSGT00390000007239.
HOGENOMiHOG000230688.
HOVERGENiHBG060425.
InParanoidiQ62141.
OMAiSEPHMVF.
OrthoDBiEOG091G03HY.
PhylomeDBiQ62141.
TreeFamiTF106187.

Family and domain databases

Gene3Di1.20.1160.11. 3 hits.
InterProiIPR013194. HDAC_interact_dom.
IPR003822. PAH.
IPR031693. Sin3_C.
[Graphical view]
PfamiPF02671. PAH. 3 hits.
PF08295. Sin3_corepress. 1 hit.
PF16879. Sin3a_C. 1 hit.
[Graphical view]
SMARTiSM00761. HDAC_interact. 1 hit.
[Graphical view]
SUPFAMiSSF47762. SSF47762. 3 hits.
PROSITEiPS51477. PAH. 3 hits.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 4 (identifier: Q62141-4) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAHAGSGGSA GRGFGGSRWG RSGSGGHEKL PVHVEDALTY LDQVKIRFGS
60 70 80 90 100
DPATYNGFLE IMKEFKSQSI DTPGVIRRVS QLFHEHPDLI VGFNAFLPLG
110 120 130 140 150
YRIDIPKNGK LNIQSPLSSQ DNSHSHGDCG EDFKQMSYKE DRGQVPLESD
160 170 180 190 200
SVEFNNAISY VNKIKTRFLD HPEIYRSFLE ILHTYQKEQL HTKGRPFRGM
210 220 230 240 250
SEEEVFTEVA NLFRGQEDLL SEFGQFLPEA KRSLFTGNGS CEMNSGQKNE
260 270 280 290 300
EKSLEHNKKR SRPSLLRPVS APAKKKMKLR GTKDLSIAAV GKYGTLQEFS
310 320 330 340 350
FFDKVRRVLK SQEVYENFLR CIALFNQELV SGSELLQLVS PFLGKFPELF
360 370 380 390 400
AQFKSFLGVK ELSFAPPMSD RSGDGISREI DYASCKRIGS SYRALPKTYQ
410 420 430 440 450
QPKCSGRTAI CKEVLNDTWV SFPSWSEDST FVSSKKTPYE EQLHRCEDER
460 470 480 490 500
FELDVVLETN LATIRVLESV QKKLSRMAPE DQEKLRLDDC LGGTSEVIQR
510 520 530 540 550
RAIHRIYGDK APEVIESLKR NPATAVPVVL KRLKAKEEEW REAQQGFNKI
560 570 580 590 600
WREQYEKAYL KSLDHQAVNF KQNDTKALRS KSLLNEIESV YDEHQEQHSE
610 620 630 640 650
GRSAPSSEPH LIFVYEDRQI LEDAAALISY YVKRQPAIQK EDQGTIRQLL
660 670 680 690 700
HRFLPSLFFS QQCPGTSDDS ADERDRDRDS AEPERRRPTD EKPPADASPE
710 720 730 740 750
PPKVLDDVYS LFFANNNWYF FLRLHQTLCA RLLKIYRQAQ KQLLEHRREQ
760 770 780 790 800
EREQLLCEGR REKAADPAME LRLKQPSEVE LEEYYPAFLD MVRSLLEGSI
810 820 830 840 850
DPTQYEDTLR EMFTIHAYIG FTMDKLVQNI ARQLHHLVSD DVCLKVVELY
860 870 880 890 900
LNEQQRGAAG GNLSSRCVRA ARETSYQWKA ERCMADENCF KVMFLQRRGQ
910 920 930 940 950
VIMTIELLDT EEAQTEDPVE VQHLARYVEQ YVGSEGASSS STEGFLLKPV
960 970 980 990 1000
FLQRNLKKFR RWQCEQVRAM RGEAKSSWKR LMGVESACDV DCRFRLGTHK
1010 1020 1030 1040 1050
MVFIVNSEDY MYRRGTLCRA KQVQPLVLLR HHRHFEEWHG RWLEDNVTVA
1060 1070 1080 1090
AAGLVQDWLM GEEEEDMVPC KTLCETAHVH GLPVTRYRVQ YSRRPASP
Note: No experimental confirmation available.
Length:1,098
Mass (Da):126,405
Last modified:June 21, 2005 - v2
Checksum:iA73F6DFC11EBA256
GO
Isoform 1 (identifier: Q62141-1) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     955-1098: Missing.

Show »
Length:954
Mass (Da):109,393
Checksum:i31F4BE14523EA213
GO
Isoform 2 (identifier: Q62141-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     275-293: KKMKLRGTKDLSIAAVGKY → VGLQLKCAVVWFGYCTAEE
     294-954: Missing.
     955-1098: Missing.

Show »
Length:293
Mass (Da):32,846
Checksum:i11A75E8F67C818AE
GO
Isoform 3 (identifier: Q62141-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     275-302: KKMKLRGTKDLSIAAVGKYGTLQEFSFF → VLVHVWVLPAHGRSGVEAQAAGEPEARA
     303-954: Missing.
     955-1098: Missing.

Note: No experimental confirmation available.
Show »
Length:302
Mass (Da):33,637
Checksum:i421131CCD87C88F8
GO

Sequence cautioni

The sequence BAD32283 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti230A → G in AAC04821 (PubMed:10620510).Curated1
Sequence conflicti233S → P in AAC04821 (PubMed:10620510).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_008227275 – 302KKMKL…EFSFF → VLVHVWVLPAHGRSGVEAQA AGEPEARA in isoform 3. 1 PublicationAdd BLAST28
Alternative sequenceiVSP_008225275 – 293KKMKL…AVGKY → VGLQLKCAVVWFGYCTAEE in isoform 2. 2 PublicationsAdd BLAST19
Alternative sequenceiVSP_008226294 – 954Missing in isoform 2. 2 PublicationsAdd BLAST661
Alternative sequenceiVSP_008228303 – 954Missing in isoform 3. 1 PublicationAdd BLAST652
Alternative sequenceiVSP_014187955 – 1098Missing in isoform 1, isoform 2 and isoform 3. 3 PublicationsAdd BLAST144

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L38622 mRNA. Translation: AAA69774.1.
AF038848 mRNA. Translation: AAC04821.1.
AK173005 mRNA. Translation: BAD32283.1. Different initiation.
BC020049 mRNA. Translation: AAH20049.1.
BC021160 mRNA. Translation: AAH21160.1.
CCDSiCCDS52598.1. [Q62141-2]
CCDS52599.1. [Q62141-4]
PIRiI61714.
RefSeqiNP_001106719.1. NM_001113248.2. [Q62141-2]
NP_033214.2. NM_009188.4. [Q62141-4]
UniGeneiMm.2137.

Genome annotation databases

EnsembliENSMUST00000004494; ENSMUSP00000004494; ENSMUSG00000031622. [Q62141-4]
ENSMUST00000109950; ENSMUSP00000105576; ENSMUSG00000031622. [Q62141-2]
ENSMUST00000212095; ENSMUSP00000148407; ENSMUSG00000031622. [Q62141-3]
GeneIDi20467.
KEGGimmu:20467.
UCSCiuc009mgp.3. mouse. [Q62141-4]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L38622 mRNA. Translation: AAA69774.1.
AF038848 mRNA. Translation: AAC04821.1.
AK173005 mRNA. Translation: BAD32283.1. Different initiation.
BC020049 mRNA. Translation: AAH20049.1.
BC021160 mRNA. Translation: AAH21160.1.
CCDSiCCDS52598.1. [Q62141-2]
CCDS52599.1. [Q62141-4]
PIRiI61714.
RefSeqiNP_001106719.1. NM_001113248.2. [Q62141-2]
NP_033214.2. NM_009188.4. [Q62141-4]
UniGeneiMm.2137.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1E91NMR-A148-232[»]
1PD7NMR-A148-232[»]
2CR7NMR-A32-98[»]
2CZYNMR-A31-107[»]
2F05NMR-A148-252[»]
ProteinModelPortaliQ62141.
SMRiQ62141.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203257. 16 interactors.
DIPiDIP-470N.
IntActiQ62141. 7 interactors.
MINTiMINT-1510576.
STRINGi10090.ENSMUSP00000004494.

PTM databases

iPTMnetiQ62141.
PhosphoSitePlusiQ62141.

Proteomic databases

MaxQBiQ62141.
PaxDbiQ62141.
PeptideAtlasiQ62141.
PRIDEiQ62141.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000004494; ENSMUSP00000004494; ENSMUSG00000031622. [Q62141-4]
ENSMUST00000109950; ENSMUSP00000105576; ENSMUSG00000031622. [Q62141-2]
ENSMUST00000212095; ENSMUSP00000148407; ENSMUSG00000031622. [Q62141-3]
GeneIDi20467.
KEGGimmu:20467.
UCSCiuc009mgp.3. mouse. [Q62141-4]

Organism-specific databases

CTDi23309.
MGIiMGI:107158. Sin3b.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG4204. Eukaryota.
COG5602. LUCA.
GeneTreeiENSGT00390000007239.
HOGENOMiHOG000230688.
HOVERGENiHBG060425.
InParanoidiQ62141.
OMAiSEPHMVF.
OrthoDBiEOG091G03HY.
PhylomeDBiQ62141.
TreeFamiTF106187.

Enzyme and pathway databases

ReactomeiR-MMU-400206. Regulation of lipid metabolism by Peroxisome proliferator-activated receptor alpha (PPARalpha).

Miscellaneous databases

ChiTaRSiSin3b. mouse.
EvolutionaryTraceiQ62141.
PROiQ62141.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000031622.
CleanExiMM_SIN3B.
ExpressionAtlasiQ62141. baseline and differential.
GenevisibleiQ62141. MM.

Family and domain databases

Gene3Di1.20.1160.11. 3 hits.
InterProiIPR013194. HDAC_interact_dom.
IPR003822. PAH.
IPR031693. Sin3_C.
[Graphical view]
PfamiPF02671. PAH. 3 hits.
PF08295. Sin3_corepress. 1 hit.
PF16879. Sin3a_C. 1 hit.
[Graphical view]
SMARTiSM00761. HDAC_interact. 1 hit.
[Graphical view]
SUPFAMiSSF47762. SSF47762. 3 hits.
PROSITEiPS51477. PAH. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSIN3B_MOUSE
AccessioniPrimary (citable) accession number: Q62141
Secondary accession number(s): O54976
, Q6A013, Q8VCB8, Q8VDZ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2003
Last sequence update: June 21, 2005
Last modified: November 30, 2016
This is version 164 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.