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Q62132 (PTPRR_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 128. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Receptor-type tyrosine-protein phosphatase R

Short name=R-PTP-R
EC=3.1.3.48
Alternative name(s):
Phosphotyrosine phosphatase 13
Protein-tyrosine-phosphatase SL
Gene names
Name:Ptprr
Synonyms:Ptp13
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length656 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Sequesters mitogen-activated protein kinases (MAPKs) such as MAPK1, MAPK3 and MAPK14 in the cytoplasm in an inactive form. The MAPKs bind to a dephosphorylated kinase interacting motif, phosphorylation of which by the protein kinase A complex releases the MAPKs for activation and translocation into the nucleus. Isoform gammamay have a role in patterning and cellular proliferation of skeletal elements in the precartilaginous/cartilaginous skeleton. Ref.4 Ref.5

Catalytic activity

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Subunit structure

Interacts with MAPKs. Ref.5

Subcellular location

Isoform Alpha: Cell membrane; Single-pass type I membrane protein Ref.5.

Isoform Beta: Cytoplasm. Note: Locates to the areas within the cytoplasm. Ref.5

Isoform Gamma: Cytoplasm. Note: Locates to the areas within the cytoplasm. Ref.5

Tissue specificity

Expressed in the heart, brain, spleen, lung, liver, skeletal muscle, kidney and testis. Isoform alphais expressed throughout the granular layer of the cerebellar but not within the Purkinje cells, also in the villi of the ileum and jejunum and both the villi and crypts of the duodenum. Isoform betais expressed only in the Purkinje cells. Isoform gammais expressed throughout the brain, the villi and crypts of the duodenum, jejunum and ileum and expressed at low levels in the proximal colon. Ref.1 Ref.2 Ref.3 Ref.4

Developmental stage

Isoform gammais the only family member developmentally expressed. Expressed throughout the brain in 15.5 day embryos and in cranial nerve cells, skeletal tissues such as neural crest-derived face bones, and the periphery of cartilaginous skeletal elements including the rib and vertebrae anlage. On day 17.5, expression was observed throughout the brain, trigeminal ganglion, cranofacial bones, oral-facial structures, cervical vertebrae, axis and the ileum. Expression continued in the vertebral column throughout ossification. Ref.4

Sequence similarities

Belongs to the protein-tyrosine phosphatase family. Receptor class 7 subfamily.

Contains 1 tyrosine-protein phosphatase domain.

Sequence caution

The sequence CAA82958.1 differs from that shown. Reason: Erroneous initiation.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Mapk1P630855EBI-6954051,EBI-397697
MAPK3P273613EBI-6954051,EBI-73995From a different organism.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform Alpha (identifier: Q62132-1)

Also known as: PTPBR7;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Beta (identifier: Q62132-2)

Also known as: PTP-SL;

The sequence of this isoform differs from the canonical sequence as follows:
     1-107: Missing.
     108-121: LPIPAANVIVVTLQ → MHRNTRSVSTPTLQ
Isoform Gamma (identifier: Q62132-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-244: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Potential
Chain24 – 656633Receptor-type tyrosine-protein phosphatase R
PRO_0000025460

Regions

Topological domain24 – 226203Extracellular Potential
Transmembrane227 – 24721Helical; Potential
Topological domain248 – 656409Cytoplasmic Potential
Domain392 – 646255Tyrosine-protein phosphatase
Region587 – 5937Substrate binding By similarity

Sites

Active site5871Phosphocysteine intermediate By similarity
Binding site5531Substrate By similarity
Binding site6311Substrate By similarity

Amino acid modifications

Modified residue3381Phosphoserine; by PKA
Glycosylation1281N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence1 – 244244Missing in isoform Gamma.
VSP_005161
Alternative sequence1 – 107107Missing in isoform Beta.
VSP_005159
Alternative sequence108 – 12114LPIPA…VVTLQ → MHRNTRSVSTPTLQ in isoform Beta.
VSP_005160

Experimental info

Mutagenesis3381S → A: Loss of phosphorylation by PKA, constitutive MAPK binding. Ref.5
Mutagenesis3381S → E: Mimics phosphorylation by PKA, prevents MAPK binding. Ref.5
Mutagenesis5871C → S: Loss of phosphatase activity. Ref.5
Mutagenesis5931R → M: Loss of phosphatase activity. Ref.5

Secondary structure

.............................................. 656
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform Alpha (PTPBR7) [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 31FA82F582992720

FASTA65674,036
        10         20         30         40         50         60 
MRRAVGFPAL CLLLNLHAAG CFSRNNDHFL AIRQKKSWKP VFIYDHSQDI KKSLDIAQEA 

        70         80         90        100        110        120 
YKHNYHSPSE VQISKHHQII NSAFPRPAYD PSLNLLAESD QDLEIENLPI PAANVIVVTL 

       130        140        150        160        170        180 
QMDITKLNIT LLRIFRQGVA AALGLLPQQV HINRLIEKKN QVELFVSPGN RKPGETQALQ 

       190        200        210        220        230        240 
AEEVLRSLNV DGLHQSLPQF GITDVAPEKN VLQGQHEADK IWSKEGFYAV VIFLSIFIII 

       250        260        270        280        290        300 
VTCLMIIYRL KERLQLSLRQ DKEKNQEIHL SPIARQQAQS EAKTTHSMVQ PDQAPKVLNV 

       310        320        330        340        350        360 
VVDPQGQCTP EIRNSTSTSV CPSPFRMKPI GLQERRGSNV SLTLDMSSLG SVEPFVAVST 

       370        380        390        400        410        420 
PREKVAMEYL QSASRVLTRS QLRDVVASSH LLQSEFMEIP MNFVDPKEID IPRHGTKNRY 

       430        440        450        460        470        480 
KTILPNPLSR VCLRPKNITD SLSTYINANY IRGYSGKEKA FIATQGPMIN TVNDFWQMVW 

       490        500        510        520        530        540 
QEDSPVIVMI TKLKEKNEKC VLYWPEKRGI YGKVEVLVTG VTECDNYTIR NLVLKQGSHT 

       550        560        570        580        590        600 
QHVKHYWYTS WPDHKTPDSA QPLLQLMLDV EEDRLASEGR GPVVVHCSAG IGRTGCFIAT 

       610        620        630        640        650 
SIGCQQLKEE GVVDALSIVC QLRVDRGGMV QTSEQYEFVH HALCLFESRL SPETVE 

« Hide

Isoform Beta (PTP-SL) [UniParc].

Checksum: C82AB43DF4C4FD7A
Show »

FASTA54961,921
Isoform Gamma [UniParc].

Checksum: C51B5F816CB8C874
Show »

FASTA41246,585

References

[1]"cDNA cloning and characterization of a novel receptor-type protein tyrosine phosphatase expressed predominantly in the brain."
Ogata M., Sawada M., Fujino Y., Hamaoka T.
J. Biol. Chem. 270:2337-2343(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM ALPHA), TISSUE SPECIFICITY.
Strain: C57BL/6.
Tissue: Brain.
[2]"A novel receptor-type protein tyrosine phosphatase with a single catalytic domain is specifically expressed in mouse brain."
Hendriks W., Schepens J., Brugman C., Zeeuwen P., Wieringa B.
Biochem. J. 305:499-504(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM BETA), TISSUE SPECIFICITY.
Strain: BALB/c.
Tissue: Brain.
[3]"Protein tyrosine phosphatase (PC12, Br7,Sl) family: expression characterization in the adult human and mouse."
Augustine K.A., Silbiger S.M., Bucay N., Ulias L., Boynton A., Trebasky L.D., Medlock E.S.
Anat. Rec. 258:221-234(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING, TISSUE SPECIFICITY.
Tissue: Colon.
[4]"Evidence that the protein tyrosine phosphatase (PC12,Br7,Sl) gamma (-) isoform modulates chondrogenic patterning and growth."
Augustine K.A., Rossi R.M., Silbiger S.M., Bucay N., Duryea D., Marshall W.S., Medlock E.S.
Int. J. Dev. Biol. 44:361-371(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM GAMMA), FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[5]"A novel regulatory mechanism of MAP kinases activation and nuclear translocation mediated by PKA and the PTP-SL tyrosine phosphatase."
Blanco-Aparicio C., Torres J., Pulido R.
J. Cell Biol. 147:1129-1136(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH MAPK1; MAPK3 AND MAPK14, SUBCELLULAR LOCATION, MUTAGENESIS OF SER-338; CYS-587 AND ARG-593.
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D31898 mRNA. Translation: BAA06696.1.
Z30313 mRNA. Translation: CAA82957.1.
Z30313 mRNA. Translation: CAA82958.1. Different initiation.
AF129509 mRNA. Translation: AAD29673.1.
AF041866 mRNA. Translation: AAD09171.1.
PIRA55574.
RefSeqNP_001155309.1. NM_001161837.1.
NP_001155310.1. NM_001161838.1.
NP_001155311.1. NM_001161839.1.
NP_001155312.1. NM_001161840.1.
NP_035347.1. NM_011217.2.
UniGeneMm.336316.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1JLNX-ray1.81A361-655[»]
ProteinModelPortalQ62132.
SMRQ62132. Positions 360-655.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ62132. 5 interactions.
MINTMINT-99987.
STRING10090.ENSMUSP00000064392.

PTM databases

PhosphoSiteQ62132.

Proteomic databases

PRIDEQ62132.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000063470; ENSMUSP00000064392; ENSMUSG00000020151. [Q62132-1]
ENSMUST00000105271; ENSMUSP00000100907; ENSMUSG00000020151. [Q62132-2]
ENSMUST00000128399; ENSMUSP00000114455; ENSMUSG00000020151. [Q62132-3]
ENSMUST00000148731; ENSMUSP00000120965; ENSMUSG00000020151. [Q62132-3]
ENSMUST00000155606; ENSMUSP00000122259; ENSMUSG00000020151. [Q62132-3]
GeneID19279.
KEGGmmu:19279.
UCSCuc007hbp.2. mouse. [Q62132-1]
uc007hbr.2. mouse. [Q62132-2]

Organism-specific databases

CTD5801.
MGIMGI:109559. Ptprr.

Phylogenomic databases

eggNOGCOG5599.
GeneTreeENSGT00750000117606.
HOGENOMHOG000294188.
HOVERGENHBG001594.
InParanoidQ62132.
KOK04458.
OMAPIGLQER.
OrthoDBEOG7288QX.
PhylomeDBQ62132.
TreeFamTF331016.

Gene expression databases

BgeeQ62132.
CleanExMM_PTPRR.
GenevestigatorQ62132.

Family and domain databases

InterProIPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
IPR008356. Tyr_Pase_KIM-con.
IPR000242. Tyr_Pase_rcpt/non-rcpt.
IPR016334. Tyr_Pase_rcpt_R/non-rcpt_5.
[Graphical view]
PfamPF00102. Y_phosphatase. 1 hit.
[Graphical view]
PIRSFPIRSF001997. PTPRR. 1 hit.
PRINTSPR01778. KIMPTPASE.
PR00700. PRTYPHPHTASE.
SMARTSM00194. PTPc. 1 hit.
[Graphical view]
PROSITEPS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50055. TYR_PHOSPHATASE_PTP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ62132.
NextBio296188.
PROQ62132.
SOURCESearch...

Entry information

Entry namePTPRR_MOUSE
AccessionPrimary (citable) accession number: Q62132
Secondary accession number(s): Q64491, Q64492, Q9QUH9
Entry history
Integrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot