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Q62132

- PTPRR_MOUSE

UniProt

Q62132 - PTPRR_MOUSE

Protein

Receptor-type tyrosine-protein phosphatase R

Gene

Ptprr

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 133 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Sequesters mitogen-activated protein kinases (MAPKs) such as MAPK1, MAPK3 and MAPK14 in the cytoplasm in an inactive form. The MAPKs bind to a dephosphorylated kinase interacting motif, phosphorylation of which by the protein kinase A complex releases the MAPKs for activation and translocation into the nucleus. Isoform gamma may have a role in patterning and cellular proliferation of skeletal elements in the precartilaginous/cartilaginous skeleton.2 Publications

    Catalytic activityi

    Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei553 – 5531SubstrateBy similarity
    Active sitei587 – 5871Phosphocysteine intermediatePROSITE-ProRule annotation
    Binding sitei631 – 6311SubstrateBy similarity

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. protein kinase binding Source: UniProtKB
    3. protein tyrosine phosphatase activity Source: MGI

    GO - Biological processi

    1. in utero embryonic development Source: UniProtKB
    2. peptidyl-tyrosine dephosphorylation Source: GOC
    3. protein dephosphorylation Source: MGI

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase, Receptor

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Receptor-type tyrosine-protein phosphatase R (EC:3.1.3.48)
    Short name:
    R-PTP-R
    Alternative name(s):
    Phosphotyrosine phosphatase 13
    Protein-tyrosine-phosphatase SL
    Gene namesi
    Name:Ptprr
    Synonyms:Ptp13
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 10

    Organism-specific databases

    MGIiMGI:109559. Ptprr.

    Subcellular locationi

    Isoform Beta : Cytoplasm
    Note: Locates to the areas within the cytoplasm.
    Isoform Gamma : Cytoplasm
    Note: Locates to the areas within the cytoplasm.

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW
    3. nucleus Source: Ensembl
    4. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi338 – 3381S → A: Loss of phosphorylation by PKA, constitutive MAPK binding. 1 Publication
    Mutagenesisi338 – 3381S → E: Mimics phosphorylation by PKA, prevents MAPK binding. 1 Publication
    Mutagenesisi587 – 5871C → S: Loss of phosphatase activity. 1 Publication
    Mutagenesisi593 – 5931R → M: Loss of phosphatase activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2323Sequence AnalysisAdd
    BLAST
    Chaini24 – 656633Receptor-type tyrosine-protein phosphatase RPRO_0000025460Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi128 – 1281N-linked (GlcNAc...)Sequence Analysis
    Modified residuei338 – 3381Phosphoserine; by PKA1 Publication

    Keywords - PTMi

    Glycoprotein, Phosphoprotein

    Proteomic databases

    PRIDEiQ62132.

    PTM databases

    PhosphoSiteiQ62132.

    Expressioni

    Tissue specificityi

    Expressed in the heart, brain, spleen, lung, liver, skeletal muscle, kidney and testis. Isoform alpha is expressed throughout the granular layer of the cerebellar but not within the Purkinje cells, also in the villi of the ileum and jejunum and both the villi and crypts of the duodenum. Isoform beta is expressed only in the Purkinje cells. Isoform gamma is expressed throughout the brain, the villi and crypts of the duodenum, jejunum and ileum and expressed at low levels in the proximal colon.4 Publications

    Developmental stagei

    Isoform gamma is the only family member developmentally expressed. Expressed throughout the brain in 15.5 day embryos and in cranial nerve cells, skeletal tissues such as neural crest-derived face bones, and the periphery of cartilaginous skeletal elements including the rib and vertebrae anlage. On day 17.5, expression was observed throughout the brain, trigeminal ganglion, cranofacial bones, oral-facial structures, cervical vertebrae, axis and the ileum. Expression continued in the vertebral column throughout ossification.1 Publication

    Gene expression databases

    BgeeiQ62132.
    CleanExiMM_PTPRR.
    GenevestigatoriQ62132.

    Interactioni

    Subunit structurei

    Interacts with MAPKs.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Mapk1P630855EBI-6954051,EBI-397697
    MAPK3P273613EBI-6954051,EBI-73995From a different organism.

    Protein-protein interaction databases

    IntActiQ62132. 5 interactions.
    MINTiMINT-99987.
    STRINGi10090.ENSMUSP00000064392.

    Structurei

    Secondary structure

    1
    656
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi363 – 37210
    Helixi379 – 3879
    Turni388 – 3903
    Helixi391 – 3988
    Turni406 – 4083
    Helixi414 – 4174
    Helixi427 – 4293
    Helixi442 – 4443
    Beta strandi447 – 4515
    Helixi454 – 4563
    Beta strandi460 – 4645
    Helixi469 – 4713
    Helixi472 – 48110
    Beta strandi486 – 4894
    Beta strandi493 – 4986
    Beta strandi506 – 5116
    Beta strandi514 – 52310
    Beta strandi525 – 53612
    Beta strandi539 – 54810
    Helixi561 – 57515
    Beta strandi583 – 5919
    Helixi592 – 61019
    Beta strandi611 – 6133
    Helixi615 – 62511
    Helixi633 – 64816

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1JLNX-ray1.81A361-655[»]
    ProteinModelPortaliQ62132.
    SMRiQ62132. Positions 360-655.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ62132.

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini24 – 226203ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini248 – 656409CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei227 – 24721HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini392 – 646255Tyrosine-protein phosphatasePROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni587 – 5937Substrate bindingBy similarity

    Sequence similaritiesi

    Contains 1 tyrosine-protein phosphatase domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG5599.
    GeneTreeiENSGT00750000117606.
    HOGENOMiHOG000294188.
    HOVERGENiHBG001594.
    InParanoidiQ62132.
    KOiK04458.
    OMAiPIGLQER.
    OrthoDBiEOG7288QX.
    PhylomeDBiQ62132.
    TreeFamiTF331016.

    Family and domain databases

    Gene3Di3.90.190.10. 1 hit.
    InterProiIPR029021. Prot-tyrosine_phosphatase-like.
    IPR000387. Tyr/Dual-sp_Pase.
    IPR016130. Tyr_Pase_AS.
    IPR008356. Tyr_Pase_KIM-con.
    IPR000242. Tyr_Pase_rcpt/non-rcpt.
    IPR016334. Tyr_Pase_rcpt_R/non-rcpt_5.
    [Graphical view]
    PfamiPF00102. Y_phosphatase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001997. PTPRR. 1 hit.
    PRINTSiPR01778. KIMPTPASE.
    PR00700. PRTYPHPHTASE.
    SMARTiSM00194. PTPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52799. SSF52799. 1 hit.
    PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
    PS50056. TYR_PHOSPHATASE_2. 1 hit.
    PS50055. TYR_PHOSPHATASE_PTP. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform Alpha (identifier: Q62132-1) [UniParc]FASTAAdd to Basket

    Also known as: PTPBR7

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MRRAVGFPAL CLLLNLHAAG CFSRNNDHFL AIRQKKSWKP VFIYDHSQDI    50
    KKSLDIAQEA YKHNYHSPSE VQISKHHQII NSAFPRPAYD PSLNLLAESD 100
    QDLEIENLPI PAANVIVVTL QMDITKLNIT LLRIFRQGVA AALGLLPQQV 150
    HINRLIEKKN QVELFVSPGN RKPGETQALQ AEEVLRSLNV DGLHQSLPQF 200
    GITDVAPEKN VLQGQHEADK IWSKEGFYAV VIFLSIFIII VTCLMIIYRL 250
    KERLQLSLRQ DKEKNQEIHL SPIARQQAQS EAKTTHSMVQ PDQAPKVLNV 300
    VVDPQGQCTP EIRNSTSTSV CPSPFRMKPI GLQERRGSNV SLTLDMSSLG 350
    SVEPFVAVST PREKVAMEYL QSASRVLTRS QLRDVVASSH LLQSEFMEIP 400
    MNFVDPKEID IPRHGTKNRY KTILPNPLSR VCLRPKNITD SLSTYINANY 450
    IRGYSGKEKA FIATQGPMIN TVNDFWQMVW QEDSPVIVMI TKLKEKNEKC 500
    VLYWPEKRGI YGKVEVLVTG VTECDNYTIR NLVLKQGSHT QHVKHYWYTS 550
    WPDHKTPDSA QPLLQLMLDV EEDRLASEGR GPVVVHCSAG IGRTGCFIAT 600
    SIGCQQLKEE GVVDALSIVC QLRVDRGGMV QTSEQYEFVH HALCLFESRL 650
    SPETVE 656
    Length:656
    Mass (Da):74,036
    Last modified:November 1, 1996 - v1
    Checksum:i31FA82F582992720
    GO
    Isoform Beta (identifier: Q62132-2) [UniParc]FASTAAdd to Basket

    Also known as: PTP-SL

    The sequence of this isoform differs from the canonical sequence as follows:
         1-107: Missing.
         108-121: LPIPAANVIVVTLQ → MHRNTRSVSTPTLQ

    Show »
    Length:549
    Mass (Da):61,921
    Checksum:iC82AB43DF4C4FD7A
    GO
    Isoform Gamma (identifier: Q62132-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-244: Missing.

    Show »
    Length:412
    Mass (Da):46,585
    Checksum:iC51B5F816CB8C874
    GO

    Sequence cautioni

    The sequence CAA82958.1 differs from that shown. Reason: Erroneous initiation.

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 244244Missing in isoform Gamma. 1 PublicationVSP_005161Add
    BLAST
    Alternative sequencei1 – 107107Missing in isoform Beta. 1 PublicationVSP_005159Add
    BLAST
    Alternative sequencei108 – 12114LPIPA…VVTLQ → MHRNTRSVSTPTLQ in isoform Beta. 1 PublicationVSP_005160Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D31898 mRNA. Translation: BAA06696.1.
    Z30313 mRNA. Translation: CAA82957.1.
    Z30313 mRNA. Translation: CAA82958.1. Different initiation.
    AF129509 mRNA. Translation: AAD29673.1.
    AF041866 mRNA. Translation: AAD09171.1.
    CCDSiCCDS24182.1. [Q62132-1]
    CCDS48693.1. [Q62132-3]
    CCDS56750.1. [Q62132-2]
    PIRiA55574.
    RefSeqiNP_001155309.1. NM_001161837.1. [Q62132-2]
    NP_001155310.1. NM_001161838.1. [Q62132-3]
    NP_001155311.1. NM_001161839.1. [Q62132-3]
    NP_001155312.1. NM_001161840.1. [Q62132-3]
    NP_035347.1. NM_011217.2. [Q62132-1]
    UniGeneiMm.336316.

    Genome annotation databases

    EnsembliENSMUST00000063470; ENSMUSP00000064392; ENSMUSG00000020151. [Q62132-1]
    ENSMUST00000105271; ENSMUSP00000100907; ENSMUSG00000020151. [Q62132-2]
    ENSMUST00000128399; ENSMUSP00000114455; ENSMUSG00000020151. [Q62132-3]
    ENSMUST00000148731; ENSMUSP00000120965; ENSMUSG00000020151. [Q62132-3]
    ENSMUST00000155606; ENSMUSP00000122259; ENSMUSG00000020151. [Q62132-3]
    GeneIDi19279.
    KEGGimmu:19279.
    UCSCiuc007hbp.2. mouse. [Q62132-1]
    uc007hbr.2. mouse. [Q62132-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D31898 mRNA. Translation: BAA06696.1 .
    Z30313 mRNA. Translation: CAA82957.1 .
    Z30313 mRNA. Translation: CAA82958.1 . Different initiation.
    AF129509 mRNA. Translation: AAD29673.1 .
    AF041866 mRNA. Translation: AAD09171.1 .
    CCDSi CCDS24182.1. [Q62132-1 ]
    CCDS48693.1. [Q62132-3 ]
    CCDS56750.1. [Q62132-2 ]
    PIRi A55574.
    RefSeqi NP_001155309.1. NM_001161837.1. [Q62132-2 ]
    NP_001155310.1. NM_001161838.1. [Q62132-3 ]
    NP_001155311.1. NM_001161839.1. [Q62132-3 ]
    NP_001155312.1. NM_001161840.1. [Q62132-3 ]
    NP_035347.1. NM_011217.2. [Q62132-1 ]
    UniGenei Mm.336316.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1JLN X-ray 1.81 A 361-655 [» ]
    ProteinModelPortali Q62132.
    SMRi Q62132. Positions 360-655.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q62132. 5 interactions.
    MINTi MINT-99987.
    STRINGi 10090.ENSMUSP00000064392.

    PTM databases

    PhosphoSitei Q62132.

    Proteomic databases

    PRIDEi Q62132.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000063470 ; ENSMUSP00000064392 ; ENSMUSG00000020151 . [Q62132-1 ]
    ENSMUST00000105271 ; ENSMUSP00000100907 ; ENSMUSG00000020151 . [Q62132-2 ]
    ENSMUST00000128399 ; ENSMUSP00000114455 ; ENSMUSG00000020151 . [Q62132-3 ]
    ENSMUST00000148731 ; ENSMUSP00000120965 ; ENSMUSG00000020151 . [Q62132-3 ]
    ENSMUST00000155606 ; ENSMUSP00000122259 ; ENSMUSG00000020151 . [Q62132-3 ]
    GeneIDi 19279.
    KEGGi mmu:19279.
    UCSCi uc007hbp.2. mouse. [Q62132-1 ]
    uc007hbr.2. mouse. [Q62132-2 ]

    Organism-specific databases

    CTDi 5801.
    MGIi MGI:109559. Ptprr.

    Phylogenomic databases

    eggNOGi COG5599.
    GeneTreei ENSGT00750000117606.
    HOGENOMi HOG000294188.
    HOVERGENi HBG001594.
    InParanoidi Q62132.
    KOi K04458.
    OMAi PIGLQER.
    OrthoDBi EOG7288QX.
    PhylomeDBi Q62132.
    TreeFami TF331016.

    Miscellaneous databases

    EvolutionaryTracei Q62132.
    NextBioi 296188.
    PROi Q62132.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q62132.
    CleanExi MM_PTPRR.
    Genevestigatori Q62132.

    Family and domain databases

    Gene3Di 3.90.190.10. 1 hit.
    InterProi IPR029021. Prot-tyrosine_phosphatase-like.
    IPR000387. Tyr/Dual-sp_Pase.
    IPR016130. Tyr_Pase_AS.
    IPR008356. Tyr_Pase_KIM-con.
    IPR000242. Tyr_Pase_rcpt/non-rcpt.
    IPR016334. Tyr_Pase_rcpt_R/non-rcpt_5.
    [Graphical view ]
    Pfami PF00102. Y_phosphatase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001997. PTPRR. 1 hit.
    PRINTSi PR01778. KIMPTPASE.
    PR00700. PRTYPHPHTASE.
    SMARTi SM00194. PTPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52799. SSF52799. 1 hit.
    PROSITEi PS00383. TYR_PHOSPHATASE_1. 1 hit.
    PS50056. TYR_PHOSPHATASE_2. 1 hit.
    PS50055. TYR_PHOSPHATASE_PTP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "cDNA cloning and characterization of a novel receptor-type protein tyrosine phosphatase expressed predominantly in the brain."
      Ogata M., Sawada M., Fujino Y., Hamaoka T.
      J. Biol. Chem. 270:2337-2343(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM ALPHA), TISSUE SPECIFICITY.
      Strain: C57BL/6.
      Tissue: Brain.
    2. "A novel receptor-type protein tyrosine phosphatase with a single catalytic domain is specifically expressed in mouse brain."
      Hendriks W., Schepens J., Brugman C., Zeeuwen P., Wieringa B.
      Biochem. J. 305:499-504(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM BETA), TISSUE SPECIFICITY.
      Strain: BALB/c.
      Tissue: Brain.
    3. "Protein tyrosine phosphatase (PC12, Br7,Sl) family: expression characterization in the adult human and mouse."
      Augustine K.A., Silbiger S.M., Bucay N., Ulias L., Boynton A., Trebasky L.D., Medlock E.S.
      Anat. Rec. 258:221-234(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING, TISSUE SPECIFICITY.
      Tissue: Colon.
    4. "Evidence that the protein tyrosine phosphatase (PC12,Br7,Sl) gamma (-) isoform modulates chondrogenic patterning and growth."
      Augustine K.A., Rossi R.M., Silbiger S.M., Bucay N., Duryea D., Marshall W.S., Medlock E.S.
      Int. J. Dev. Biol. 44:361-371(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM GAMMA), FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    5. "A novel regulatory mechanism of MAP kinases activation and nuclear translocation mediated by PKA and the PTP-SL tyrosine phosphatase."
      Blanco-Aparicio C., Torres J., Pulido R.
      J. Cell Biol. 147:1129-1136(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH MAPK1; MAPK3 AND MAPK14, SUBCELLULAR LOCATION, PHOSPHORYLATION AT SER-338, MUTAGENESIS OF SER-338; CYS-587 AND ARG-593.
      Tissue: Brain.

    Entry informationi

    Entry nameiPTPRR_MOUSE
    AccessioniPrimary (citable) accession number: Q62132
    Secondary accession number(s): Q64491, Q64492, Q9QUH9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 15, 2002
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 133 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3