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Protein

Receptor-type tyrosine-protein phosphatase R

Gene

Ptprr

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Sequesters mitogen-activated protein kinases (MAPKs) such as MAPK1, MAPK3 and MAPK14 in the cytoplasm in an inactive form. The MAPKs bind to a dephosphorylated kinase interacting motif, phosphorylation of which by the protein kinase A complex releases the MAPKs for activation and translocation into the nucleus. Isoform gamma may have a role in patterning and cellular proliferation of skeletal elements in the precartilaginous/cartilaginous skeleton.2 Publications

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei553SubstrateBy similarity1
Active sitei587Phosphocysteine intermediatePROSITE-ProRule annotation1
Binding sitei631SubstrateBy similarity1

GO - Molecular functioni

  • protein kinase binding Source: UniProtKB
  • protein tyrosine phosphatase activity Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase, Receptor

Names & Taxonomyi

Protein namesi
Recommended name:
Receptor-type tyrosine-protein phosphatase R (EC:3.1.3.48)
Short name:
R-PTP-R
Alternative name(s):
Phosphotyrosine phosphatase 13
Protein-tyrosine-phosphatase SL
Gene namesi
Name:Ptprr
Synonyms:Ptp13
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:109559. Ptprr.

Subcellular locationi

Isoform Beta :
  • Cytoplasm

  • Note: Locates to the areas within the cytoplasm.
Isoform Gamma :
  • Cytoplasm

  • Note: Locates to the areas within the cytoplasm.

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini24 – 226ExtracellularSequence analysisAdd BLAST203
Transmembranei227 – 247HelicalSequence analysisAdd BLAST21
Topological domaini248 – 656CytoplasmicSequence analysisAdd BLAST409

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi338S → A: Loss of phosphorylation by PKA, constitutive MAPK binding. 1 Publication1
Mutagenesisi338S → E: Mimics phosphorylation by PKA, prevents MAPK binding. 1 Publication1
Mutagenesisi587C → S: Loss of phosphatase activity. 1 Publication1
Mutagenesisi593R → M: Loss of phosphatase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
ChainiPRO_000002546024 – 656Receptor-type tyrosine-protein phosphatase RAdd BLAST633

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi128N-linked (GlcNAc...)Sequence analysis1
Modified residuei271PhosphoserineCombined sources1
Modified residuei338Phosphoserine; by PKA1 Publication1

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ62132.
PRIDEiQ62132.

PTM databases

iPTMnetiQ62132.
PhosphoSitePlusiQ62132.

Expressioni

Tissue specificityi

Expressed in the heart, brain, spleen, lung, liver, skeletal muscle, kidney and testis. Isoform alpha is expressed throughout the granular layer of the cerebellar but not within the Purkinje cells, also in the villi of the ileum and jejunum and both the villi and crypts of the duodenum. Isoform beta is expressed only in the Purkinje cells. Isoform gamma is expressed throughout the brain, the villi and crypts of the duodenum, jejunum and ileum and expressed at low levels in the proximal colon.4 Publications

Developmental stagei

Isoform gamma is the only family member developmentally expressed. Expressed throughout the brain in 15.5 day embryos and in cranial nerve cells, skeletal tissues such as neural crest-derived face bones, and the periphery of cartilaginous skeletal elements including the rib and vertebrae anlage. On day 17.5, expression was observed throughout the brain, trigeminal ganglion, cranofacial bones, oral-facial structures, cervical vertebrae, axis and the ileum. Expression continued in the vertebral column throughout ossification.1 Publication

Gene expression databases

BgeeiENSMUSG00000020151.
CleanExiMM_PTPRR.
ExpressionAtlasiQ62132. baseline and differential.
GenevisibleiQ62132. MM.

Interactioni

Subunit structurei

Interacts with MAPKs.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Mapk1P630855EBI-6954051,EBI-397697
MAPK3P273613EBI-6954051,EBI-73995From a different organism.

GO - Molecular functioni

  • protein kinase binding Source: UniProtKB

Protein-protein interaction databases

IntActiQ62132. 5 interactors.
MINTiMINT-99987.
STRINGi10090.ENSMUSP00000064392.

Structurei

Secondary structure

1656
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi363 – 372Combined sources10
Helixi379 – 387Combined sources9
Turni388 – 390Combined sources3
Helixi391 – 398Combined sources8
Turni406 – 408Combined sources3
Helixi414 – 417Combined sources4
Helixi427 – 429Combined sources3
Helixi442 – 444Combined sources3
Beta strandi447 – 451Combined sources5
Helixi454 – 456Combined sources3
Beta strandi460 – 464Combined sources5
Helixi469 – 471Combined sources3
Helixi472 – 481Combined sources10
Beta strandi486 – 489Combined sources4
Beta strandi493 – 498Combined sources6
Beta strandi506 – 511Combined sources6
Beta strandi514 – 523Combined sources10
Beta strandi525 – 536Combined sources12
Beta strandi539 – 548Combined sources10
Helixi561 – 575Combined sources15
Beta strandi583 – 591Combined sources9
Helixi592 – 610Combined sources19
Beta strandi611 – 613Combined sources3
Helixi615 – 625Combined sources11
Helixi633 – 648Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1JLNX-ray1.81A361-655[»]
ProteinModelPortaliQ62132.
SMRiQ62132.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ62132.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini392 – 646Tyrosine-protein phosphatasePROSITE-ProRule annotationAdd BLAST255

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni587 – 593Substrate bindingBy similarity7

Sequence similaritiesi

Contains 1 tyrosine-protein phosphatase domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0789. Eukaryota.
COG5599. LUCA.
GeneTreeiENSGT00770000120452.
HOGENOMiHOG000294188.
HOVERGENiHBG001594.
InParanoidiQ62132.
KOiK04458.
OMAiCLMILYR.
OrthoDBiEOG091G0HLB.
PhylomeDBiQ62132.
TreeFamiTF331016.

Family and domain databases

Gene3Di3.90.190.10. 1 hit.
InterProiIPR029021. Prot-tyrosine_phosphatase-like.
IPR000242. PTPase_domain.
IPR016130. Tyr_Pase_AS.
IPR003595. Tyr_Pase_cat.
IPR008356. Tyr_Pase_KIM-con.
IPR016334. Tyr_Pase_rcpt_R/non-rcpt_5.
IPR000387. TYR_PHOSPHATASE_dom.
[Graphical view]
PfamiPF00102. Y_phosphatase. 1 hit.
[Graphical view]
PIRSFiPIRSF001997. PTPRR. 1 hit.
PRINTSiPR01778. KIMPTPASE.
PR00700. PRTYPHPHTASE.
SMARTiSM00194. PTPc. 1 hit.
SM00404. PTPc_motif. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.
PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50055. TYR_PHOSPHATASE_PTP. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Alpha (identifier: Q62132-1) [UniParc]FASTAAdd to basket
Also known as: PTPBR7

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRRAVGFPAL CLLLNLHAAG CFSRNNDHFL AIRQKKSWKP VFIYDHSQDI
60 70 80 90 100
KKSLDIAQEA YKHNYHSPSE VQISKHHQII NSAFPRPAYD PSLNLLAESD
110 120 130 140 150
QDLEIENLPI PAANVIVVTL QMDITKLNIT LLRIFRQGVA AALGLLPQQV
160 170 180 190 200
HINRLIEKKN QVELFVSPGN RKPGETQALQ AEEVLRSLNV DGLHQSLPQF
210 220 230 240 250
GITDVAPEKN VLQGQHEADK IWSKEGFYAV VIFLSIFIII VTCLMIIYRL
260 270 280 290 300
KERLQLSLRQ DKEKNQEIHL SPIARQQAQS EAKTTHSMVQ PDQAPKVLNV
310 320 330 340 350
VVDPQGQCTP EIRNSTSTSV CPSPFRMKPI GLQERRGSNV SLTLDMSSLG
360 370 380 390 400
SVEPFVAVST PREKVAMEYL QSASRVLTRS QLRDVVASSH LLQSEFMEIP
410 420 430 440 450
MNFVDPKEID IPRHGTKNRY KTILPNPLSR VCLRPKNITD SLSTYINANY
460 470 480 490 500
IRGYSGKEKA FIATQGPMIN TVNDFWQMVW QEDSPVIVMI TKLKEKNEKC
510 520 530 540 550
VLYWPEKRGI YGKVEVLVTG VTECDNYTIR NLVLKQGSHT QHVKHYWYTS
560 570 580 590 600
WPDHKTPDSA QPLLQLMLDV EEDRLASEGR GPVVVHCSAG IGRTGCFIAT
610 620 630 640 650
SIGCQQLKEE GVVDALSIVC QLRVDRGGMV QTSEQYEFVH HALCLFESRL

SPETVE
Length:656
Mass (Da):74,036
Last modified:November 1, 1996 - v1
Checksum:i31FA82F582992720
GO
Isoform Beta (identifier: Q62132-2) [UniParc]FASTAAdd to basket
Also known as: PTP-SL

The sequence of this isoform differs from the canonical sequence as follows:
     1-107: Missing.
     108-121: LPIPAANVIVVTLQ → MHRNTRSVSTPTLQ

Show »
Length:549
Mass (Da):61,921
Checksum:iC82AB43DF4C4FD7A
GO
Isoform Gamma (identifier: Q62132-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-244: Missing.

Show »
Length:412
Mass (Da):46,585
Checksum:iC51B5F816CB8C874
GO

Sequence cautioni

The sequence CAA82958 differs from that shown. Reason: Erroneous initiation.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0051611 – 244Missing in isoform Gamma. 1 PublicationAdd BLAST244
Alternative sequenceiVSP_0051591 – 107Missing in isoform Beta. 1 PublicationAdd BLAST107
Alternative sequenceiVSP_005160108 – 121LPIPA…VVTLQ → MHRNTRSVSTPTLQ in isoform Beta. 1 PublicationAdd BLAST14

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D31898 mRNA. Translation: BAA06696.1.
Z30313 mRNA. Translation: CAA82957.1.
Z30313 mRNA. Translation: CAA82958.1. Different initiation.
AF129509 mRNA. Translation: AAD29673.1.
AF041866 mRNA. Translation: AAD09171.1.
CCDSiCCDS24182.1. [Q62132-1]
CCDS48693.1. [Q62132-3]
CCDS56750.1. [Q62132-2]
PIRiA55574.
RefSeqiNP_001155309.1. NM_001161837.1. [Q62132-2]
NP_001155310.1. NM_001161838.1. [Q62132-3]
NP_001155311.1. NM_001161839.1. [Q62132-3]
NP_001155312.1. NM_001161840.1. [Q62132-3]
NP_035347.1. NM_011217.2. [Q62132-1]
UniGeneiMm.336316.

Genome annotation databases

EnsembliENSMUST00000063470; ENSMUSP00000064392; ENSMUSG00000020151. [Q62132-1]
ENSMUST00000105271; ENSMUSP00000100907; ENSMUSG00000020151. [Q62132-2]
ENSMUST00000128399; ENSMUSP00000114455; ENSMUSG00000020151. [Q62132-3]
ENSMUST00000148731; ENSMUSP00000120965; ENSMUSG00000020151. [Q62132-3]
ENSMUST00000155606; ENSMUSP00000122259; ENSMUSG00000020151. [Q62132-3]
GeneIDi19279.
KEGGimmu:19279.
UCSCiuc007hbp.2. mouse. [Q62132-1]
uc007hbr.2. mouse. [Q62132-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D31898 mRNA. Translation: BAA06696.1.
Z30313 mRNA. Translation: CAA82957.1.
Z30313 mRNA. Translation: CAA82958.1. Different initiation.
AF129509 mRNA. Translation: AAD29673.1.
AF041866 mRNA. Translation: AAD09171.1.
CCDSiCCDS24182.1. [Q62132-1]
CCDS48693.1. [Q62132-3]
CCDS56750.1. [Q62132-2]
PIRiA55574.
RefSeqiNP_001155309.1. NM_001161837.1. [Q62132-2]
NP_001155310.1. NM_001161838.1. [Q62132-3]
NP_001155311.1. NM_001161839.1. [Q62132-3]
NP_001155312.1. NM_001161840.1. [Q62132-3]
NP_035347.1. NM_011217.2. [Q62132-1]
UniGeneiMm.336316.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1JLNX-ray1.81A361-655[»]
ProteinModelPortaliQ62132.
SMRiQ62132.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ62132. 5 interactors.
MINTiMINT-99987.
STRINGi10090.ENSMUSP00000064392.

PTM databases

iPTMnetiQ62132.
PhosphoSitePlusiQ62132.

Proteomic databases

PaxDbiQ62132.
PRIDEiQ62132.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000063470; ENSMUSP00000064392; ENSMUSG00000020151. [Q62132-1]
ENSMUST00000105271; ENSMUSP00000100907; ENSMUSG00000020151. [Q62132-2]
ENSMUST00000128399; ENSMUSP00000114455; ENSMUSG00000020151. [Q62132-3]
ENSMUST00000148731; ENSMUSP00000120965; ENSMUSG00000020151. [Q62132-3]
ENSMUST00000155606; ENSMUSP00000122259; ENSMUSG00000020151. [Q62132-3]
GeneIDi19279.
KEGGimmu:19279.
UCSCiuc007hbp.2. mouse. [Q62132-1]
uc007hbr.2. mouse. [Q62132-2]

Organism-specific databases

CTDi5801.
MGIiMGI:109559. Ptprr.

Phylogenomic databases

eggNOGiKOG0789. Eukaryota.
COG5599. LUCA.
GeneTreeiENSGT00770000120452.
HOGENOMiHOG000294188.
HOVERGENiHBG001594.
InParanoidiQ62132.
KOiK04458.
OMAiCLMILYR.
OrthoDBiEOG091G0HLB.
PhylomeDBiQ62132.
TreeFamiTF331016.

Miscellaneous databases

EvolutionaryTraceiQ62132.
PROiQ62132.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020151.
CleanExiMM_PTPRR.
ExpressionAtlasiQ62132. baseline and differential.
GenevisibleiQ62132. MM.

Family and domain databases

Gene3Di3.90.190.10. 1 hit.
InterProiIPR029021. Prot-tyrosine_phosphatase-like.
IPR000242. PTPase_domain.
IPR016130. Tyr_Pase_AS.
IPR003595. Tyr_Pase_cat.
IPR008356. Tyr_Pase_KIM-con.
IPR016334. Tyr_Pase_rcpt_R/non-rcpt_5.
IPR000387. TYR_PHOSPHATASE_dom.
[Graphical view]
PfamiPF00102. Y_phosphatase. 1 hit.
[Graphical view]
PIRSFiPIRSF001997. PTPRR. 1 hit.
PRINTSiPR01778. KIMPTPASE.
PR00700. PRTYPHPHTASE.
SMARTiSM00194. PTPc. 1 hit.
SM00404. PTPc_motif. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.
PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50055. TYR_PHOSPHATASE_PTP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPTPRR_MOUSE
AccessioniPrimary (citable) accession number: Q62132
Secondary accession number(s): Q64491, Q64492, Q9QUH9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 151 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.