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Protein

Receptor-type tyrosine-protein phosphatase R

Gene

Ptprr

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Sequesters mitogen-activated protein kinases (MAPKs) such as MAPK1, MAPK3 and MAPK14 in the cytoplasm in an inactive form. The MAPKs bind to a dephosphorylated kinase interacting motif, phosphorylation of which by the protein kinase A complex releases the MAPKs for activation and translocation into the nucleus. Isoform gamma may have a role in patterning and cellular proliferation of skeletal elements in the precartilaginous/cartilaginous skeleton.2 Publications

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei553 – 5531SubstrateBy similarity
Active sitei587 – 5871Phosphocysteine intermediatePROSITE-ProRule annotation
Binding sitei631 – 6311SubstrateBy similarity

GO - Molecular functioni

  • protein kinase binding Source: UniProtKB
  • protein tyrosine phosphatase activity Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase, Receptor

Names & Taxonomyi

Protein namesi
Recommended name:
Receptor-type tyrosine-protein phosphatase R (EC:3.1.3.48)
Short name:
R-PTP-R
Alternative name(s):
Phosphotyrosine phosphatase 13
Protein-tyrosine-phosphatase SL
Gene namesi
Name:Ptprr
Synonyms:Ptp13
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:109559. Ptprr.

Subcellular locationi

Isoform Beta :
  • Cytoplasm

  • Note: Locates to the areas within the cytoplasm.
Isoform Gamma :
  • Cytoplasm

  • Note: Locates to the areas within the cytoplasm.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini24 – 226203ExtracellularSequence AnalysisAdd
BLAST
Transmembranei227 – 24721HelicalSequence AnalysisAdd
BLAST
Topological domaini248 – 656409CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi338 – 3381S → A: Loss of phosphorylation by PKA, constitutive MAPK binding. 1 Publication
Mutagenesisi338 – 3381S → E: Mimics phosphorylation by PKA, prevents MAPK binding. 1 Publication
Mutagenesisi587 – 5871C → S: Loss of phosphatase activity. 1 Publication
Mutagenesisi593 – 5931R → M: Loss of phosphatase activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence AnalysisAdd
BLAST
Chaini24 – 656633Receptor-type tyrosine-protein phosphatase RPRO_0000025460Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi128 – 1281N-linked (GlcNAc...)Sequence Analysis
Modified residuei338 – 3381Phosphoserine; by PKA1 Publication

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PRIDEiQ62132.

PTM databases

PhosphoSiteiQ62132.

Expressioni

Tissue specificityi

Expressed in the heart, brain, spleen, lung, liver, skeletal muscle, kidney and testis. Isoform alpha is expressed throughout the granular layer of the cerebellar but not within the Purkinje cells, also in the villi of the ileum and jejunum and both the villi and crypts of the duodenum. Isoform beta is expressed only in the Purkinje cells. Isoform gamma is expressed throughout the brain, the villi and crypts of the duodenum, jejunum and ileum and expressed at low levels in the proximal colon.4 Publications

Developmental stagei

Isoform gamma is the only family member developmentally expressed. Expressed throughout the brain in 15.5 day embryos and in cranial nerve cells, skeletal tissues such as neural crest-derived face bones, and the periphery of cartilaginous skeletal elements including the rib and vertebrae anlage. On day 17.5, expression was observed throughout the brain, trigeminal ganglion, cranofacial bones, oral-facial structures, cervical vertebrae, axis and the ileum. Expression continued in the vertebral column throughout ossification.1 Publication

Gene expression databases

BgeeiQ62132.
CleanExiMM_PTPRR.
ExpressionAtlasiQ62132. baseline and differential.
GenevestigatoriQ62132.

Interactioni

Subunit structurei

Interacts with MAPKs.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Mapk1P630855EBI-6954051,EBI-397697
MAPK3P273613EBI-6954051,EBI-73995From a different organism.

Protein-protein interaction databases

IntActiQ62132. 5 interactions.
MINTiMINT-99987.
STRINGi10090.ENSMUSP00000064392.

Structurei

Secondary structure

1
656
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi363 – 37210Combined sources
Helixi379 – 3879Combined sources
Turni388 – 3903Combined sources
Helixi391 – 3988Combined sources
Turni406 – 4083Combined sources
Helixi414 – 4174Combined sources
Helixi427 – 4293Combined sources
Helixi442 – 4443Combined sources
Beta strandi447 – 4515Combined sources
Helixi454 – 4563Combined sources
Beta strandi460 – 4645Combined sources
Helixi469 – 4713Combined sources
Helixi472 – 48110Combined sources
Beta strandi486 – 4894Combined sources
Beta strandi493 – 4986Combined sources
Beta strandi506 – 5116Combined sources
Beta strandi514 – 52310Combined sources
Beta strandi525 – 53612Combined sources
Beta strandi539 – 54810Combined sources
Helixi561 – 57515Combined sources
Beta strandi583 – 5919Combined sources
Helixi592 – 61019Combined sources
Beta strandi611 – 6133Combined sources
Helixi615 – 62511Combined sources
Helixi633 – 64816Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1JLNX-ray1.81A361-655[»]
ProteinModelPortaliQ62132.
SMRiQ62132. Positions 360-655.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ62132.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini392 – 646255Tyrosine-protein phosphatasePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni587 – 5937Substrate bindingBy similarity

Sequence similaritiesi

Contains 1 tyrosine-protein phosphatase domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG5599.
GeneTreeiENSGT00780000121945.
HOGENOMiHOG000294188.
HOVERGENiHBG001594.
InParanoidiQ62132.
KOiK04458.
OMAiIVTCLMI.
OrthoDBiEOG7288QX.
PhylomeDBiQ62132.
TreeFamiTF331016.

Family and domain databases

Gene3Di3.90.190.10. 1 hit.
InterProiIPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
IPR008356. Tyr_Pase_KIM-con.
IPR000242. Tyr_Pase_rcpt/non-rcpt.
IPR016334. Tyr_Pase_rcpt_R/non-rcpt_5.
[Graphical view]
PfamiPF00102. Y_phosphatase. 1 hit.
[Graphical view]
PIRSFiPIRSF001997. PTPRR. 1 hit.
PRINTSiPR01778. KIMPTPASE.
PR00700. PRTYPHPHTASE.
SMARTiSM00194. PTPc. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.
PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50055. TYR_PHOSPHATASE_PTP. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Alpha (identifier: Q62132-1) [UniParc]FASTAAdd to basket

Also known as: PTPBR7

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRRAVGFPAL CLLLNLHAAG CFSRNNDHFL AIRQKKSWKP VFIYDHSQDI
60 70 80 90 100
KKSLDIAQEA YKHNYHSPSE VQISKHHQII NSAFPRPAYD PSLNLLAESD
110 120 130 140 150
QDLEIENLPI PAANVIVVTL QMDITKLNIT LLRIFRQGVA AALGLLPQQV
160 170 180 190 200
HINRLIEKKN QVELFVSPGN RKPGETQALQ AEEVLRSLNV DGLHQSLPQF
210 220 230 240 250
GITDVAPEKN VLQGQHEADK IWSKEGFYAV VIFLSIFIII VTCLMIIYRL
260 270 280 290 300
KERLQLSLRQ DKEKNQEIHL SPIARQQAQS EAKTTHSMVQ PDQAPKVLNV
310 320 330 340 350
VVDPQGQCTP EIRNSTSTSV CPSPFRMKPI GLQERRGSNV SLTLDMSSLG
360 370 380 390 400
SVEPFVAVST PREKVAMEYL QSASRVLTRS QLRDVVASSH LLQSEFMEIP
410 420 430 440 450
MNFVDPKEID IPRHGTKNRY KTILPNPLSR VCLRPKNITD SLSTYINANY
460 470 480 490 500
IRGYSGKEKA FIATQGPMIN TVNDFWQMVW QEDSPVIVMI TKLKEKNEKC
510 520 530 540 550
VLYWPEKRGI YGKVEVLVTG VTECDNYTIR NLVLKQGSHT QHVKHYWYTS
560 570 580 590 600
WPDHKTPDSA QPLLQLMLDV EEDRLASEGR GPVVVHCSAG IGRTGCFIAT
610 620 630 640 650
SIGCQQLKEE GVVDALSIVC QLRVDRGGMV QTSEQYEFVH HALCLFESRL

SPETVE
Length:656
Mass (Da):74,036
Last modified:November 1, 1996 - v1
Checksum:i31FA82F582992720
GO
Isoform Beta (identifier: Q62132-2) [UniParc]FASTAAdd to basket

Also known as: PTP-SL

The sequence of this isoform differs from the canonical sequence as follows:
     1-107: Missing.
     108-121: LPIPAANVIVVTLQ → MHRNTRSVSTPTLQ

Show »
Length:549
Mass (Da):61,921
Checksum:iC82AB43DF4C4FD7A
GO
Isoform Gamma (identifier: Q62132-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-244: Missing.

Show »
Length:412
Mass (Da):46,585
Checksum:iC51B5F816CB8C874
GO

Sequence cautioni

The sequence CAA82958.1 differs from that shown. Reason: Erroneous initiation. Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 244244Missing in isoform Gamma. 1 PublicationVSP_005161Add
BLAST
Alternative sequencei1 – 107107Missing in isoform Beta. 1 PublicationVSP_005159Add
BLAST
Alternative sequencei108 – 12114LPIPA…VVTLQ → MHRNTRSVSTPTLQ in isoform Beta. 1 PublicationVSP_005160Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D31898 mRNA. Translation: BAA06696.1.
Z30313 mRNA. Translation: CAA82957.1.
Z30313 mRNA. Translation: CAA82958.1. Different initiation.
AF129509 mRNA. Translation: AAD29673.1.
AF041866 mRNA. Translation: AAD09171.1.
CCDSiCCDS24182.1. [Q62132-1]
CCDS48693.1. [Q62132-3]
CCDS56750.1. [Q62132-2]
PIRiA55574.
RefSeqiNP_001155309.1. NM_001161837.1. [Q62132-2]
NP_001155310.1. NM_001161838.1. [Q62132-3]
NP_001155311.1. NM_001161839.1. [Q62132-3]
NP_001155312.1. NM_001161840.1. [Q62132-3]
NP_035347.1. NM_011217.2. [Q62132-1]
UniGeneiMm.336316.

Genome annotation databases

EnsembliENSMUST00000063470; ENSMUSP00000064392; ENSMUSG00000020151. [Q62132-1]
ENSMUST00000105271; ENSMUSP00000100907; ENSMUSG00000020151. [Q62132-2]
ENSMUST00000128399; ENSMUSP00000114455; ENSMUSG00000020151. [Q62132-3]
ENSMUST00000148731; ENSMUSP00000120965; ENSMUSG00000020151. [Q62132-3]
ENSMUST00000155606; ENSMUSP00000122259; ENSMUSG00000020151. [Q62132-3]
GeneIDi19279.
KEGGimmu:19279.
UCSCiuc007hbp.2. mouse. [Q62132-1]
uc007hbr.2. mouse. [Q62132-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D31898 mRNA. Translation: BAA06696.1.
Z30313 mRNA. Translation: CAA82957.1.
Z30313 mRNA. Translation: CAA82958.1. Different initiation.
AF129509 mRNA. Translation: AAD29673.1.
AF041866 mRNA. Translation: AAD09171.1.
CCDSiCCDS24182.1. [Q62132-1]
CCDS48693.1. [Q62132-3]
CCDS56750.1. [Q62132-2]
PIRiA55574.
RefSeqiNP_001155309.1. NM_001161837.1. [Q62132-2]
NP_001155310.1. NM_001161838.1. [Q62132-3]
NP_001155311.1. NM_001161839.1. [Q62132-3]
NP_001155312.1. NM_001161840.1. [Q62132-3]
NP_035347.1. NM_011217.2. [Q62132-1]
UniGeneiMm.336316.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1JLNX-ray1.81A361-655[»]
ProteinModelPortaliQ62132.
SMRiQ62132. Positions 360-655.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ62132. 5 interactions.
MINTiMINT-99987.
STRINGi10090.ENSMUSP00000064392.

PTM databases

PhosphoSiteiQ62132.

Proteomic databases

PRIDEiQ62132.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000063470; ENSMUSP00000064392; ENSMUSG00000020151. [Q62132-1]
ENSMUST00000105271; ENSMUSP00000100907; ENSMUSG00000020151. [Q62132-2]
ENSMUST00000128399; ENSMUSP00000114455; ENSMUSG00000020151. [Q62132-3]
ENSMUST00000148731; ENSMUSP00000120965; ENSMUSG00000020151. [Q62132-3]
ENSMUST00000155606; ENSMUSP00000122259; ENSMUSG00000020151. [Q62132-3]
GeneIDi19279.
KEGGimmu:19279.
UCSCiuc007hbp.2. mouse. [Q62132-1]
uc007hbr.2. mouse. [Q62132-2]

Organism-specific databases

CTDi5801.
MGIiMGI:109559. Ptprr.

Phylogenomic databases

eggNOGiCOG5599.
GeneTreeiENSGT00780000121945.
HOGENOMiHOG000294188.
HOVERGENiHBG001594.
InParanoidiQ62132.
KOiK04458.
OMAiIVTCLMI.
OrthoDBiEOG7288QX.
PhylomeDBiQ62132.
TreeFamiTF331016.

Miscellaneous databases

EvolutionaryTraceiQ62132.
NextBioi296188.
PROiQ62132.
SOURCEiSearch...

Gene expression databases

BgeeiQ62132.
CleanExiMM_PTPRR.
ExpressionAtlasiQ62132. baseline and differential.
GenevestigatoriQ62132.

Family and domain databases

Gene3Di3.90.190.10. 1 hit.
InterProiIPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
IPR008356. Tyr_Pase_KIM-con.
IPR000242. Tyr_Pase_rcpt/non-rcpt.
IPR016334. Tyr_Pase_rcpt_R/non-rcpt_5.
[Graphical view]
PfamiPF00102. Y_phosphatase. 1 hit.
[Graphical view]
PIRSFiPIRSF001997. PTPRR. 1 hit.
PRINTSiPR01778. KIMPTPASE.
PR00700. PRTYPHPHTASE.
SMARTiSM00194. PTPc. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.
PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50055. TYR_PHOSPHATASE_PTP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "cDNA cloning and characterization of a novel receptor-type protein tyrosine phosphatase expressed predominantly in the brain."
    Ogata M., Sawada M., Fujino Y., Hamaoka T.
    J. Biol. Chem. 270:2337-2343(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM ALPHA), TISSUE SPECIFICITY.
    Strain: C57BL/6.
    Tissue: Brain.
  2. "A novel receptor-type protein tyrosine phosphatase with a single catalytic domain is specifically expressed in mouse brain."
    Hendriks W., Schepens J., Brugman C., Zeeuwen P., Wieringa B.
    Biochem. J. 305:499-504(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM BETA), TISSUE SPECIFICITY.
    Strain: BALB/c.
    Tissue: Brain.
  3. "Protein tyrosine phosphatase (PC12, Br7,Sl) family: expression characterization in the adult human and mouse."
    Augustine K.A., Silbiger S.M., Bucay N., Ulias L., Boynton A., Trebasky L.D., Medlock E.S.
    Anat. Rec. 258:221-234(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING, TISSUE SPECIFICITY.
    Tissue: Colon.
  4. "Evidence that the protein tyrosine phosphatase (PC12,Br7,Sl) gamma (-) isoform modulates chondrogenic patterning and growth."
    Augustine K.A., Rossi R.M., Silbiger S.M., Bucay N., Duryea D., Marshall W.S., Medlock E.S.
    Int. J. Dev. Biol. 44:361-371(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM GAMMA), FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  5. "A novel regulatory mechanism of MAP kinases activation and nuclear translocation mediated by PKA and the PTP-SL tyrosine phosphatase."
    Blanco-Aparicio C., Torres J., Pulido R.
    J. Cell Biol. 147:1129-1136(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH MAPK1; MAPK3 AND MAPK14, SUBCELLULAR LOCATION, PHOSPHORYLATION AT SER-338, MUTAGENESIS OF SER-338; CYS-587 AND ARG-593.
    Tissue: Brain.

Entry informationi

Entry nameiPTPRR_MOUSE
AccessioniPrimary (citable) accession number: Q62132
Secondary accession number(s): Q64491, Q64492, Q9QUH9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: November 1, 1996
Last modified: February 4, 2015
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.