Tyrosine-protein phosphatase non-receptor type 14

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Q62130 (PTN14_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 103. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Tyrosine-protein phosphatase non-receptor type 14
EC=3.1.3.48

Alternative name(s):
Protein-tyrosine phosphatase PTP36

Gene names

Name:

Ptpn14

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1189 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Protein tyrosine phosphatase which may play a role in the regulation of lymphangiogenesis, cell-cell adhesion, cell-matrix adhesion, cell migration, cell growth and also regulates TGF-beta gene expression, thereby modulating epithelial-mesenchymal transition. Mediates beta-catenin dephosphorylation at adhesion junctions. Acts as a negative regulator of the oncogenic property of YAP, a downstream target of the hippo pathway, in a cell density-dependent manner. May function as a tumor suppressor. Ref.5
Catalytic activity	Protein tyrosine phosphate + H₂O = protein tyrosine + phosphate.
Subunit structure	Interacts with FLT4; the interaction is enhanced by stimulation with VEGFC By similarity. Interacts (via PPxY motifs) with YAP1 (via WW domains); this interaction leads to the cytoplasmic sequestration of YAP1 and inhibits its transcriptional coactivator activity By similarity.
Subcellular location	Cytoplasm By similarity. Cytoplasm › cytoskeleton By similarity. Nucleus By similarity. Note: Translocation into the nucleus is associated with induction of cell proliferation By similarity. Partially colocalized with actin filaments at the plasma membrane By similarity.
Tissue specificity	Thymus; in cells of both hematopoietic and non-hematopoietic origins.
Post-translational modification	Ubiquitinated by the ECS (Elongin BC-CUL2/5-SOCS-box protein)/LRR1 E3 ligase complex and subsequently targeted to proteasomal degradation By similarity.
Disruption phenotype	PTPN14 deficient mice have swelling of the limbs or periorbital edema. These mice also show hyperplasia of lymphatic capillaries of the ears. There is no evidence of choanal atresia or any overtly dysmorphic features. Ref.5
Sequence similarities	Belongs to the protein-tyrosine phosphatase family. Non-receptor class subfamily. Contains 1 FERM domain. Contains 1 tyrosine-protein phosphatase domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm Cytoskeleton Nucleus
Molecular function	Hydrolase Protein phosphatase
PTM	Phosphoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	lymphangiogenesis Inferred from mutant phenotype Ref.5. Source: UniProtKB regulation of protein export from nucleus Inferred from sequence or structural similarity. Source: UniProtKB regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB cytoskeleton Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	protein tyrosine phosphatase activity Inferred from sequence or structural similarity. Source: UniProtKB transcription cofactor activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1189

1189

Tyrosine-protein phosphatase non-receptor type 14

PRO_0000219438

Regions

Domain

21 – 306

286

FERM

Domain

911 – 1182

272

Tyrosine-protein phosphatase

Region

1123 – 1129

Substrate binding By similarity

Compositional bias

566 – 573

Poly-Pro

Compositional bias

635 – 639

Poly-Gly

Compositional bias

712 – 718

Poly-Glu

Sites

Active site

1123

Phosphocysteine intermediate By similarity

Binding site

1167

Substrate By similarity

Amino acid modifications

Modified residue

486

Phosphoserine By similarity

Modified residue

591

Phosphoserine By similarity

Modified residue

593

Phosphoserine Ref.4

Modified residue

594

Phosphoserine Ref.4

Modified residue

646

Phosphoserine By similarity

Experimental info

Sequence conflict

T → A in BAA06628. Ref.1

Sequence conflict

887

H → R in BAA06628. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

Q62130 [UniParc].

Last modified March 6, 2013. Version 2.
Checksum: 7FAFED60C5145477
Show »

FASTA

1,189

135,042

        10         20         30         40         50         60 
MPFGLKLRRT RRYNVLSKNC FVTRIRLLDS NVIECTLSVE STGQECLEAV AQRLELRETH 

        70         80         90        100        110        120 
YFGLWFLSKS QQARWVELEK PLKKHLDKFA NEPLLFFGVM FYVPNVSRLQ QEATRYQYYL 

       130        140        150        160        170        180 
QVKKDVLEGR LRCSLEQVIR LAGLAVQADF GDYNQFDSQE FLREYVLFPM DLAMEEAALE 

       190        200        210        220        230        240 
ELTQKVAQEH KAHSGILPAE AELMYINEVE RLDGFGQEIF PVKDSHGNSV HLGIFFMGIF 

       250        260        270        280        290        300 
VRNRVGRQAV IYRWNDIGSV THSKAAILLE LIDKEETALF HTDDIENAKY ISRLFTTRHK 

       310        320        330        340        350        360 
FYKQNKICTE QSNSPPPIRR QPTWSRSSLP RQQPYILPPM HVQCSEHYSE THTSQDSIFP 

       370        380        390        400        410        420 
GNEEALYCRS HNSLDLNYLN GTVTNGSVCS VHSVNSLSCS QSFIQASPVS SNLSIPGSDI 

       430        440        450        460        470        480 
MRADYIPSHR HSTIIVPSYR PTPDYETVMR QMKRGLMHAD SQSRSLRNLN IINTHAYNQP 

       490        500        510        520        530        540 
EELVYSQPEM RERHPYTVPY AHQGCYGHKL VSPSDQMNPQ NCAMPIKPGA SSISHTVSTP 

       550        560        570        580        590        600 
ELANMQLQGA QHYSTAHMLK NYLFRPPPPY PRPRPATSTP DLASHRHKYV SGSSPDLVTR 

       610        620        630        640        650        660 
KVQLSVKTFQ EDSSPVVHQS LQEVSEPLTA TKHHGGGGGT VNKRHSLEVM NSMVRGMEAM 

       670        680        690        700        710        720 
TLKSLNIPMA RRNTLREQGP SEETGGHEVH GLPQYHHKKT FSDATMLIHS SESEEEEETL 

       730        740        750        760        770        780 
EAAPQVPVLR EKVEYSAQLQ AALARIPNRP PPEYPGPRKS VSNGALRQDQ GTPLPAMARC 

       790        800        810        820        830        840 
RVLRHGPSKA LSVSRAEQLA VNGASLGPSI SEPDLTSVKE RVKKEPVKER PVSEMFSLED 

       850        860        870        880        890        900 
SIIEREMMIR NLEKQKMTGP QAQKRPLMLA ALNGLSVARV SGREDGHHDA TRVPIDERLR 

       910        920        930        940        950        960 
ALKKKLEDGM VFTEYEQIPN KKANGVFSTA TLPENAERSR IREVVPYEEN RVELIPTKEN 

       970        980        990       1000       1010       1020 
NTGYINASHI KVVVGGSEWH YIATQGPLPH TCHDFWQMVW EQGVNVIAMV TAEEEGGRTK 

      1030       1040       1050       1060       1070       1080 
SHRYWPKLGS KHSSATYGKF KVTTKFRTDS GCYATTGLKV KHLLSGQERT VWHLQYTDWP 

      1090       1100       1110       1120       1130       1140 
HHGCPEDVQG FLSYLEEIQS VRRHTNSVLE GIRTRHPPIV VHCSAGVGRT GVVILSELMI 

      1150       1160       1170       1180 
YCLEHNEKVE VPTMLRFLRE QRMFMIQTIA QYKFVYQVLV QFLQNSRLI

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"cDNA cloning of a novel protein tyrosine phosphatase with homology to cytoskeletal protein 4.1 and its expression in T-lineage cells." Sawada M., Ogata M., Fujino Y., Hamaoka T. Biochem. Biophys. Res. Commun. 203:479-484(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: CB-17/SCID. Tissue: Thymus.
[2]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[3]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-593 AND SER-594, MASS SPECTROMETRY. Tissue: Liver.
[5]	"Protein tyrosine phosphatase PTPN14 is a regulator of lymphatic function and choanal development in humans." Au A.C., Hernandez P.A., Lieber E., Nadroo A.M., Shen Y.M., Kelley K.A., Gelb B.D., Diaz G.A. Am. J. Hum. Genet. 87:436-444(2010) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D31842 mRNA. Translation: BAA06628.1. AC140250 Genomic DNA. No translation available. AC140461 Genomic DNA. No translation available. CH466555 Genomic DNA. Translation: EDL13025.1. CH466555 Genomic DNA. Translation: EDL13028.1.
IPI	IPI00122168.
PIR	JC2366.
RefSeq	NP_033002.2. NM_008976.2.
UniGene	Mm.4498.
3D structure databases
ProteinModelPortal	Q62130.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q62130. 1 interaction.
PTM databases
PhosphoSite	Q62130.
Proteomic databases
PaxDb	Q62130.
PRIDE	Q62130.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000027898; ENSMUSP00000027898; ENSMUSG00000026604. ENSMUST00000097442; ENSMUSP00000095051; ENSMUSG00000026604.
GeneID	19250.
UCSC	uc007eau.1. mouse.
Organism-specific databases
CTD	5784.
MGI	MGI:102467. Ptpn14.
Phylogenomic databases
eggNOG	COG5599.
GeneTree	ENSGT00700000104164.
HOGENOM	HOG000115775.
HOVERGEN	HBG053757.
InParanoid	Q62130.
OrthoDB	EOG4320X9.
Gene expression databases
Genevestigator	Q62130.
GermOnline	ENSMUSG00000026604. Mus musculus.
Family and domain databases
Gene3D	1.20.80.10. 1 hit. 2.30.29.30. 1 hit.
InterPro	IPR019749. Band_41_domain. IPR019750. Band_41_fam. IPR014352. FERM/acyl-CoA-bd_prot_3-hlx. IPR019748. FERM_central. IPR019747. FERM_CS. IPR000299. FERM_domain. IPR018979. FERM_N. IPR018980. FERM_PH-like_C. IPR011993. PH_like_dom. IPR000387. Tyr/Dual-sp_Pase. IPR016130. Tyr_Pase_AS. IPR014392. Tyr_Pase_non-rcpt_typ-14/21. IPR000242. Tyr_Pase_rcpt/non-rcpt. [Graphical view]
Pfam	PF09380. FERM_C. 1 hit. PF00373. FERM_M. 1 hit. PF09379. FERM_N. 1 hit. PF00102. Y_phosphatase. 1 hit. [Graphical view]
PIRSF	PIRSF000934. Tyr-Ptase_nr14. 1 hit.
PRINTS	PR00935. BAND41. PR00700. PRTYPHPHTASE.
SMART	SM00295. B41. 1 hit. SM00194. PTPc. 1 hit. [Graphical view]
SUPFAM	SSF47031. FERM_3-hlx. 1 hit.
PROSITE	PS00660. FERM_1. 1 hit. PS00661. FERM_2. 1 hit. PS50057. FERM_3. 1 hit. PS00383. TYR_PHOSPHATASE_1. 1 hit. PS50056. TYR_PHOSPHATASE_2. 1 hit. PS50055. TYR_PHOSPHATASE_PTP. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	296086.
SOURCE	Search...

Entry information

Entry name

PTN14_MOUSE

Accession

Primary (citable) accession number: Q62130
Secondary accession number(s): G5E8M1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	March 6, 2013
Last modified:	May 1, 2013
This is version 103 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents