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Q62130 (PTN14_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 115. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Tyrosine-protein phosphatase non-receptor type 14

EC=3.1.3.48
Alternative name(s):
Protein-tyrosine phosphatase PTP36
Gene names
Name:Ptpn14
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1189 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Protein tyrosine phosphatase which may play a role in the regulation of lymphangiogenesis, cell-cell adhesion, cell-matrix adhesion, cell migration, cell growth and also regulates TGF-beta gene expression, thereby modulating epithelial-mesenchymal transition. Mediates beta-catenin dephosphorylation at adhesion junctions. Acts as a negative regulator of the oncogenic property of YAP, a downstream target of the hippo pathway, in a cell density-dependent manner. May function as a tumor suppressor. Ref.4

Catalytic activity

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Subunit structure

Interacts with FLT4; the interaction is enhanced by stimulation with VEGFC By similarity. Interacts (via PPxY motifs) with YAP1 (via WW domains); this interaction leads to the cytoplasmic sequestration of YAP1 and inhibits its transcriptional coactivator activity By similarity.

Subcellular location

Cytoplasm By similarity. Cytoplasmcytoskeleton By similarity. Nucleus By similarity. Note: Translocation into the nucleus is associated with induction of cell proliferation By similarity. Partially colocalized with actin filaments at the plasma membrane By similarity.

Tissue specificity

Thymus; in cells of both hematopoietic and non-hematopoietic origins.

Post-translational modification

Ubiquitinated by the ECS (Elongin BC-CUL2/5-SOCS-box protein)/LRR1 E3 ligase complex and subsequently targeted to proteasomal degradation By similarity.

Disruption phenotype

PTPN14 deficient mice have swelling of the limbs or periorbital edema. These mice also show hyperplasia of lymphatic capillaries of the ears. There is no evidence of choanal atresia or any overtly dysmorphic features. Ref.4

Sequence similarities

Belongs to the protein-tyrosine phosphatase family. Non-receptor class subfamily.

Contains 1 FERM domain.

Contains 1 tyrosine-protein phosphatase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11891189Tyrosine-protein phosphatase non-receptor type 14
PRO_0000219438

Regions

Domain21 – 306286FERM
Domain911 – 1182272Tyrosine-protein phosphatase
Region1123 – 11297Substrate binding By similarity
Compositional bias566 – 5738Poly-Pro
Compositional bias635 – 6395Poly-Gly
Compositional bias712 – 7187Poly-Glu

Sites

Active site11231Phosphocysteine intermediate By similarity
Binding site11671Substrate By similarity

Amino acid modifications

Modified residue4861Phosphoserine By similarity
Modified residue5911Phosphoserine By similarity
Modified residue5941Phosphoserine By similarity
Modified residue6461Phosphoserine By similarity

Experimental info

Sequence conflict231T → A in BAA06628. Ref.1
Sequence conflict8871H → R in BAA06628. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q62130 [UniParc].

Last modified March 6, 2013. Version 2.
Checksum: 7FAFED60C5145477

FASTA1,189135,042
        10         20         30         40         50         60 
MPFGLKLRRT RRYNVLSKNC FVTRIRLLDS NVIECTLSVE STGQECLEAV AQRLELRETH 

        70         80         90        100        110        120 
YFGLWFLSKS QQARWVELEK PLKKHLDKFA NEPLLFFGVM FYVPNVSRLQ QEATRYQYYL 

       130        140        150        160        170        180 
QVKKDVLEGR LRCSLEQVIR LAGLAVQADF GDYNQFDSQE FLREYVLFPM DLAMEEAALE 

       190        200        210        220        230        240 
ELTQKVAQEH KAHSGILPAE AELMYINEVE RLDGFGQEIF PVKDSHGNSV HLGIFFMGIF 

       250        260        270        280        290        300 
VRNRVGRQAV IYRWNDIGSV THSKAAILLE LIDKEETALF HTDDIENAKY ISRLFTTRHK 

       310        320        330        340        350        360 
FYKQNKICTE QSNSPPPIRR QPTWSRSSLP RQQPYILPPM HVQCSEHYSE THTSQDSIFP 

       370        380        390        400        410        420 
GNEEALYCRS HNSLDLNYLN GTVTNGSVCS VHSVNSLSCS QSFIQASPVS SNLSIPGSDI 

       430        440        450        460        470        480 
MRADYIPSHR HSTIIVPSYR PTPDYETVMR QMKRGLMHAD SQSRSLRNLN IINTHAYNQP 

       490        500        510        520        530        540 
EELVYSQPEM RERHPYTVPY AHQGCYGHKL VSPSDQMNPQ NCAMPIKPGA SSISHTVSTP 

       550        560        570        580        590        600 
ELANMQLQGA QHYSTAHMLK NYLFRPPPPY PRPRPATSTP DLASHRHKYV SGSSPDLVTR 

       610        620        630        640        650        660 
KVQLSVKTFQ EDSSPVVHQS LQEVSEPLTA TKHHGGGGGT VNKRHSLEVM NSMVRGMEAM 

       670        680        690        700        710        720 
TLKSLNIPMA RRNTLREQGP SEETGGHEVH GLPQYHHKKT FSDATMLIHS SESEEEEETL 

       730        740        750        760        770        780 
EAAPQVPVLR EKVEYSAQLQ AALARIPNRP PPEYPGPRKS VSNGALRQDQ GTPLPAMARC 

       790        800        810        820        830        840 
RVLRHGPSKA LSVSRAEQLA VNGASLGPSI SEPDLTSVKE RVKKEPVKER PVSEMFSLED 

       850        860        870        880        890        900 
SIIEREMMIR NLEKQKMTGP QAQKRPLMLA ALNGLSVARV SGREDGHHDA TRVPIDERLR 

       910        920        930        940        950        960 
ALKKKLEDGM VFTEYEQIPN KKANGVFSTA TLPENAERSR IREVVPYEEN RVELIPTKEN 

       970        980        990       1000       1010       1020 
NTGYINASHI KVVVGGSEWH YIATQGPLPH TCHDFWQMVW EQGVNVIAMV TAEEEGGRTK 

      1030       1040       1050       1060       1070       1080 
SHRYWPKLGS KHSSATYGKF KVTTKFRTDS GCYATTGLKV KHLLSGQERT VWHLQYTDWP 

      1090       1100       1110       1120       1130       1140 
HHGCPEDVQG FLSYLEEIQS VRRHTNSVLE GIRTRHPPIV VHCSAGVGRT GVVILSELMI 

      1150       1160       1170       1180 
YCLEHNEKVE VPTMLRFLRE QRMFMIQTIA QYKFVYQVLV QFLQNSRLI 

« Hide

References

« Hide 'large scale' references
[1]"cDNA cloning of a novel protein tyrosine phosphatase with homology to cytoskeletal protein 4.1 and its expression in T-lineage cells."
Sawada M., Ogata M., Fujino Y., Hamaoka T.
Biochem. Biophys. Res. Commun. 203:479-484(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: CB-17/SCID.
Tissue: Thymus.
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[3]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"Protein tyrosine phosphatase PTPN14 is a regulator of lymphatic function and choanal development in humans."
Au A.C., Hernandez P.A., Lieber E., Nadroo A.M., Shen Y.M., Kelley K.A., Gelb B.D., Diaz G.A.
Am. J. Hum. Genet. 87:436-444(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D31842 mRNA. Translation: BAA06628.1.
AC140250 Genomic DNA. No translation available.
AC140461 Genomic DNA. No translation available.
CH466555 Genomic DNA. Translation: EDL13025.1.
CH466555 Genomic DNA. Translation: EDL13028.1.
CCDSCCDS15609.1.
PIRJC2366.
RefSeqNP_033002.2. NM_008976.2.
XP_006497192.1. XM_006497129.1.
XP_006497193.1. XM_006497130.1.
XP_006497194.1. XM_006497131.1.
XP_006497195.1. XM_006497132.1.
XP_006497196.1. XM_006497133.1.
UniGeneMm.4498.

3D structure databases

ProteinModelPortalQ62130.
SMRQ62130. Positions 24-303, 897-1186.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ62130. 1 interaction.

PTM databases

PhosphoSiteQ62130.

Proteomic databases

PaxDbQ62130.
PRIDEQ62130.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000027898; ENSMUSP00000027898; ENSMUSG00000026604.
ENSMUST00000097442; ENSMUSP00000095051; ENSMUSG00000026604.
GeneID19250.
KEGGmmu:19250.
UCSCuc007eau.1. mouse.

Organism-specific databases

CTD5784.
MGIMGI:102467. Ptpn14.

Phylogenomic databases

eggNOGCOG5599.
GeneTreeENSGT00750000117681.
HOGENOMHOG000115775.
HOVERGENHBG053757.
InParanoidQ62130.
KOK18025.
OMAVTTKFRT.
OrthoDBEOG7PK8XS.
TreeFamTF315900.

Gene expression databases

ArrayExpressQ62130.
GenevestigatorQ62130.

Family and domain databases

Gene3D1.20.80.10. 1 hit.
2.30.29.30. 1 hit.
3.90.190.10. 1 hit.
InterProIPR019749. Band_41_domain.
IPR019750. Band_41_fam.
IPR014352. FERM/acyl-CoA-bd_prot_3-hlx.
IPR019748. FERM_central.
IPR019747. FERM_CS.
IPR000299. FERM_domain.
IPR018979. FERM_N.
IPR018980. FERM_PH-like_C.
IPR011993. PH_like_dom.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
IPR014392. Tyr_Pase_non-rcpt_typ-14/21.
IPR000242. Tyr_Pase_rcpt/non-rcpt.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamPF09380. FERM_C. 1 hit.
PF00373. FERM_M. 1 hit.
PF09379. FERM_N. 1 hit.
PF00102. Y_phosphatase. 1 hit.
[Graphical view]
PIRSFPIRSF000934. Tyr-Ptase_nr14. 1 hit.
PRINTSPR00935. BAND41.
PR00700. PRTYPHPHTASE.
SMARTSM00295. B41. 1 hit.
SM00194. PTPc. 1 hit.
[Graphical view]
SUPFAMSSF47031. SSF47031. 1 hit.
SSF52799. SSF52799. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEPS00660. FERM_1. 1 hit.
PS00661. FERM_2. 1 hit.
PS50057. FERM_3. 1 hit.
PS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50055. TYR_PHOSPHATASE_PTP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio296086.
PROQ62130.
SOURCESearch...

Entry information

Entry namePTN14_MOUSE
AccessionPrimary (citable) accession number: Q62130
Secondary accession number(s): G5E8M1
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: March 6, 2013
Last modified: July 9, 2014
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot