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Protein

Tyrosine-protein phosphatase non-receptor type 14

Gene

Ptpn14

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Protein tyrosine phosphatase which may play a role in the regulation of lymphangiogenesis, cell-cell adhesion, cell-matrix adhesion, cell migration, cell growth and also regulates TGF-beta gene expression, thereby modulating epithelial-mesenchymal transition. Mediates beta-catenin dephosphorylation at adhesion junctions. Acts as a negative regulator of the oncogenic property of YAP, a downstream target of the hippo pathway, in a cell density-dependent manner. May function as a tumor suppressor.1 Publication

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei1123 – 11231Phosphocysteine intermediatePROSITE-ProRule annotation
Binding sitei1167 – 11671SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

BRENDAi3.1.3.48. 3474.

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine-protein phosphatase non-receptor type 14 (EC:3.1.3.48)
Alternative name(s):
Protein-tyrosine phosphatase PTP36
Gene namesi
Name:Ptpn14
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:102467. Ptpn14.

Subcellular locationi

  • Cytoplasm By similarity
  • Cytoplasmcytoskeleton By similarity
  • Nucleus By similarity

  • Note: Translocation into the nucleus is associated with induction of cell proliferation. Partially colocalized with actin filaments at the plasma membrane.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

Pathology & Biotechi

Disruption phenotypei

PTPN14 deficient mice have swelling of the limbs or periorbital edema. These mice also show hyperplasia of lymphatic capillaries of the ears. There is no evidence of choanal atresia or any overtly dysmorphic features.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11891189Tyrosine-protein phosphatase non-receptor type 14PRO_0000219438Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei486 – 4861PhosphoserineBy similarity
Modified residuei591 – 5911PhosphoserineBy similarity
Modified residuei594 – 5941PhosphoserineBy similarity
Modified residuei646 – 6461PhosphoserineBy similarity

Post-translational modificationi

Ubiquitinated by the ECS (Elongin BC-CUL2/5-SOCS-box protein)/LRR1 E3 ligase complex and subsequently targeted to proteasomal degradation.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ62130.
PRIDEiQ62130.

PTM databases

PhosphoSiteiQ62130.

Expressioni

Tissue specificityi

Thymus; in cells of both hematopoietic and non-hematopoietic origins.

Gene expression databases

ExpressionAtlasiQ62130. baseline and differential.

Interactioni

Subunit structurei

Interacts with FLT4; the interaction is enhanced by stimulation with VEGFC. Interacts (via PPxY motifs) with YAP1 (via WW domains); this interaction leads to the cytoplasmic sequestration of YAP1 and inhibits its transcriptional coactivator activity.By similarity

Protein-protein interaction databases

BioGridi202480. 1 interaction.
IntActiQ62130. 1 interaction.
STRINGi10090.ENSMUSP00000027898.

Structurei

3D structure databases

ProteinModelPortaliQ62130.
SMRiQ62130. Positions 24-303, 897-1186.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini21 – 306286FERMPROSITE-ProRule annotationAdd
BLAST
Domaini911 – 1182272Tyrosine-protein phosphatasePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1123 – 11297Substrate bindingBy similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi566 – 5738Poly-Pro
Compositional biasi635 – 6395Poly-Gly
Compositional biasi712 – 7187Poly-Glu

Sequence similaritiesi

Contains 1 FERM domain.PROSITE-ProRule annotation
Contains 1 tyrosine-protein phosphatase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5599.
GeneTreeiENSGT00780000121879.
HOGENOMiHOG000115775.
HOVERGENiHBG053757.
InParanoidiQ62130.
KOiK18025.
OMAiVTTKFRT.
OrthoDBiEOG7PK8XS.
TreeFamiTF315900.

Family and domain databases

Gene3Di1.20.80.10. 1 hit.
2.30.29.30. 1 hit.
3.90.190.10. 1 hit.
InterProiIPR019749. Band_41_domain.
IPR014352. FERM/acyl-CoA-bd_prot_3-hlx.
IPR019748. FERM_central.
IPR019747. FERM_CS.
IPR000299. FERM_domain.
IPR018979. FERM_N.
IPR018980. FERM_PH-like_C.
IPR011993. PH_like_dom.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
IPR014392. Tyr_Pase_non-rcpt_typ-14/21.
IPR000242. Tyr_Pase_rcpt/non-rcpt.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF09380. FERM_C. 1 hit.
PF00373. FERM_M. 1 hit.
PF09379. FERM_N. 1 hit.
PF00102. Y_phosphatase. 1 hit.
[Graphical view]
PIRSFiPIRSF000934. Tyr-Ptase_nr14. 1 hit.
PRINTSiPR00935. BAND41.
PR00700. PRTYPHPHTASE.
SMARTiSM00295. B41. 1 hit.
SM00194. PTPc. 1 hit.
[Graphical view]
SUPFAMiSSF47031. SSF47031. 1 hit.
SSF52799. SSF52799. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEiPS00660. FERM_1. 1 hit.
PS00661. FERM_2. 1 hit.
PS50057. FERM_3. 1 hit.
PS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50055. TYR_PHOSPHATASE_PTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q62130-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPFGLKLRRT RRYNVLSKNC FVTRIRLLDS NVIECTLSVE STGQECLEAV
60 70 80 90 100
AQRLELRETH YFGLWFLSKS QQARWVELEK PLKKHLDKFA NEPLLFFGVM
110 120 130 140 150
FYVPNVSRLQ QEATRYQYYL QVKKDVLEGR LRCSLEQVIR LAGLAVQADF
160 170 180 190 200
GDYNQFDSQE FLREYVLFPM DLAMEEAALE ELTQKVAQEH KAHSGILPAE
210 220 230 240 250
AELMYINEVE RLDGFGQEIF PVKDSHGNSV HLGIFFMGIF VRNRVGRQAV
260 270 280 290 300
IYRWNDIGSV THSKAAILLE LIDKEETALF HTDDIENAKY ISRLFTTRHK
310 320 330 340 350
FYKQNKICTE QSNSPPPIRR QPTWSRSSLP RQQPYILPPM HVQCSEHYSE
360 370 380 390 400
THTSQDSIFP GNEEALYCRS HNSLDLNYLN GTVTNGSVCS VHSVNSLSCS
410 420 430 440 450
QSFIQASPVS SNLSIPGSDI MRADYIPSHR HSTIIVPSYR PTPDYETVMR
460 470 480 490 500
QMKRGLMHAD SQSRSLRNLN IINTHAYNQP EELVYSQPEM RERHPYTVPY
510 520 530 540 550
AHQGCYGHKL VSPSDQMNPQ NCAMPIKPGA SSISHTVSTP ELANMQLQGA
560 570 580 590 600
QHYSTAHMLK NYLFRPPPPY PRPRPATSTP DLASHRHKYV SGSSPDLVTR
610 620 630 640 650
KVQLSVKTFQ EDSSPVVHQS LQEVSEPLTA TKHHGGGGGT VNKRHSLEVM
660 670 680 690 700
NSMVRGMEAM TLKSLNIPMA RRNTLREQGP SEETGGHEVH GLPQYHHKKT
710 720 730 740 750
FSDATMLIHS SESEEEEETL EAAPQVPVLR EKVEYSAQLQ AALARIPNRP
760 770 780 790 800
PPEYPGPRKS VSNGALRQDQ GTPLPAMARC RVLRHGPSKA LSVSRAEQLA
810 820 830 840 850
VNGASLGPSI SEPDLTSVKE RVKKEPVKER PVSEMFSLED SIIEREMMIR
860 870 880 890 900
NLEKQKMTGP QAQKRPLMLA ALNGLSVARV SGREDGHHDA TRVPIDERLR
910 920 930 940 950
ALKKKLEDGM VFTEYEQIPN KKANGVFSTA TLPENAERSR IREVVPYEEN
960 970 980 990 1000
RVELIPTKEN NTGYINASHI KVVVGGSEWH YIATQGPLPH TCHDFWQMVW
1010 1020 1030 1040 1050
EQGVNVIAMV TAEEEGGRTK SHRYWPKLGS KHSSATYGKF KVTTKFRTDS
1060 1070 1080 1090 1100
GCYATTGLKV KHLLSGQERT VWHLQYTDWP HHGCPEDVQG FLSYLEEIQS
1110 1120 1130 1140 1150
VRRHTNSVLE GIRTRHPPIV VHCSAGVGRT GVVILSELMI YCLEHNEKVE
1160 1170 1180
VPTMLRFLRE QRMFMIQTIA QYKFVYQVLV QFLQNSRLI
Length:1,189
Mass (Da):135,042
Last modified:March 6, 2013 - v2
Checksum:i7FAFED60C5145477
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti23 – 231T → A in BAA06628 (PubMed:8074693).Curated
Sequence conflicti887 – 8871H → R in BAA06628 (PubMed:8074693).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D31842 mRNA. Translation: BAA06628.1.
AC140250 Genomic DNA. No translation available.
AC140461 Genomic DNA. No translation available.
CH466555 Genomic DNA. Translation: EDL13025.1.
CH466555 Genomic DNA. Translation: EDL13028.1.
CCDSiCCDS15609.1.
PIRiJC2366.
RefSeqiNP_033002.2. NM_008976.2.
XP_006497192.1. XM_006497129.2.
XP_006497193.1. XM_006497130.2.
XP_006497194.1. XM_006497131.2.
XP_006497195.1. XM_006497132.1.
XP_006497196.1. XM_006497133.2.
UniGeneiMm.4498.

Genome annotation databases

EnsembliENSMUST00000097442; ENSMUSP00000095051; ENSMUSG00000026604.
GeneIDi19250.
KEGGimmu:19250.
UCSCiuc007eau.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D31842 mRNA. Translation: BAA06628.1.
AC140250 Genomic DNA. No translation available.
AC140461 Genomic DNA. No translation available.
CH466555 Genomic DNA. Translation: EDL13025.1.
CH466555 Genomic DNA. Translation: EDL13028.1.
CCDSiCCDS15609.1.
PIRiJC2366.
RefSeqiNP_033002.2. NM_008976.2.
XP_006497192.1. XM_006497129.2.
XP_006497193.1. XM_006497130.2.
XP_006497194.1. XM_006497131.2.
XP_006497195.1. XM_006497132.1.
XP_006497196.1. XM_006497133.2.
UniGeneiMm.4498.

3D structure databases

ProteinModelPortaliQ62130.
SMRiQ62130. Positions 24-303, 897-1186.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202480. 1 interaction.
IntActiQ62130. 1 interaction.
STRINGi10090.ENSMUSP00000027898.

PTM databases

PhosphoSiteiQ62130.

Proteomic databases

PaxDbiQ62130.
PRIDEiQ62130.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000097442; ENSMUSP00000095051; ENSMUSG00000026604.
GeneIDi19250.
KEGGimmu:19250.
UCSCiuc007eau.1. mouse.

Organism-specific databases

CTDi5784.
MGIiMGI:102467. Ptpn14.

Phylogenomic databases

eggNOGiCOG5599.
GeneTreeiENSGT00780000121879.
HOGENOMiHOG000115775.
HOVERGENiHBG053757.
InParanoidiQ62130.
KOiK18025.
OMAiVTTKFRT.
OrthoDBiEOG7PK8XS.
TreeFamiTF315900.

Enzyme and pathway databases

BRENDAi3.1.3.48. 3474.

Miscellaneous databases

ChiTaRSiPtpn14. mouse.
NextBioi296086.
PROiQ62130.
SOURCEiSearch...

Gene expression databases

ExpressionAtlasiQ62130. baseline and differential.

Family and domain databases

Gene3Di1.20.80.10. 1 hit.
2.30.29.30. 1 hit.
3.90.190.10. 1 hit.
InterProiIPR019749. Band_41_domain.
IPR014352. FERM/acyl-CoA-bd_prot_3-hlx.
IPR019748. FERM_central.
IPR019747. FERM_CS.
IPR000299. FERM_domain.
IPR018979. FERM_N.
IPR018980. FERM_PH-like_C.
IPR011993. PH_like_dom.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
IPR014392. Tyr_Pase_non-rcpt_typ-14/21.
IPR000242. Tyr_Pase_rcpt/non-rcpt.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF09380. FERM_C. 1 hit.
PF00373. FERM_M. 1 hit.
PF09379. FERM_N. 1 hit.
PF00102. Y_phosphatase. 1 hit.
[Graphical view]
PIRSFiPIRSF000934. Tyr-Ptase_nr14. 1 hit.
PRINTSiPR00935. BAND41.
PR00700. PRTYPHPHTASE.
SMARTiSM00295. B41. 1 hit.
SM00194. PTPc. 1 hit.
[Graphical view]
SUPFAMiSSF47031. SSF47031. 1 hit.
SSF52799. SSF52799. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEiPS00660. FERM_1. 1 hit.
PS00661. FERM_2. 1 hit.
PS50057. FERM_3. 1 hit.
PS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50055. TYR_PHOSPHATASE_PTP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "cDNA cloning of a novel protein tyrosine phosphatase with homology to cytoskeletal protein 4.1 and its expression in T-lineage cells."
    Sawada M., Ogata M., Fujino Y., Hamaoka T.
    Biochem. Biophys. Res. Commun. 203:479-484(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: CB-17/SCID.
    Tissue: Thymus.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "Protein tyrosine phosphatase PTPN14 is a regulator of lymphatic function and choanal development in humans."
    Au A.C., Hernandez P.A., Lieber E., Nadroo A.M., Shen Y.M., Kelley K.A., Gelb B.D., Diaz G.A.
    Am. J. Hum. Genet. 87:436-444(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiPTN14_MOUSE
AccessioniPrimary (citable) accession number: Q62130
Secondary accession number(s): G5E8M1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: March 6, 2013
Last modified: June 24, 2015
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.