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Protein

Serine/arginine-rich splicing factor 2

Gene

Srsf2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Necessary for the splicing of pre-mRNA. It is required for formation of the earliest ATP-dependent splicing complex and interacts with spliceosomal components bound to both the 5'- and 3'-splice sites during spliceosome assembly. It also is required for ATP-dependent interactions of both U1 and U2 snRNPs with pre-mRNA (By similarity). Can bind to the myelin basic protein (MBP) gene MB3 regulatory region and increase transcription of the mbp promoter in cells derived from the CNS. The phosphorylated form (by SRPK2) is required for cellular apoptosis in response to cisplatin treatment (By similarity).By similarity1 Publication

GO - Molecular functioni

  • pre-mRNA binding Source: MGI

GO - Biological processi

Keywordsi

Molecular functionRNA-binding
Biological processmRNA processing, mRNA splicing

Enzyme and pathway databases

ReactomeiR-MMU-109688 Cleavage of Growing Transcript in the Termination Region
R-MMU-159236 Transport of Mature mRNA derived from an Intron-Containing Transcript
R-MMU-72163 mRNA Splicing - Major Pathway
R-MMU-72165 mRNA Splicing - Minor Pathway
R-MMU-72187 mRNA 3'-end processing

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/arginine-rich splicing factor 2
Alternative name(s):
Protein PR264
Putative myelin regulatory factor 1
Short name:
MRF-1
Splicing component, 35 kDa
Splicing factor SC35
Short name:
SC-35
Splicing factor, arginine/serine-rich 2
Gene namesi
Name:Srsf2
Synonyms:Pr264, Sfrs10, Sfrs2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:98284 Srsf2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000819192 – 221Serine/arginine-rich splicing factor 2Add BLAST220

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei2PhosphoserineBy similarity1
Modified residuei22PhosphothreonineBy similarity1
Modified residuei25PhosphothreonineBy similarity1
Modified residuei26PhosphoserineCombined sources1
Modified residuei52N6-acetyllysineBy similarity1
Modified residuei189PhosphoserineBy similarity1
Modified residuei191PhosphoserineBy similarity1
Modified residuei204PhosphoserineBy similarity1
Modified residuei206PhosphoserineCombined sources1
Modified residuei208PhosphoserineCombined sources1
Modified residuei212PhosphoserineCombined sources1
Modified residuei220PhosphoserineBy similarity1

Post-translational modificationi

Extensively phosphorylated on serine residues in the RS domain. Phosphorylated by SRPK2 and this causes its redistribution from the nuclear speckle to nucleoplasm and controls cell fate decision in response to cisplatin treatment. KAT5/TIP60 inhibits its phosphorylation by preventing SRPK2 nuclear translocation (By similarity).By similarity
Acetylation on Lys-52 by KAT5/TIP60 promotes its proteasomal degradation. This effect is counterbalanced by HDAC6, which positively controls SRSF2 protein level by deacetylating it and preventing its proteasomal degradation (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ62093
MaxQBiQ62093
PaxDbiQ62093
PeptideAtlasiQ62093
PRIDEiQ62093

PTM databases

iPTMnetiQ62093
PhosphoSitePlusiQ62093

Expressioni

Tissue specificityi

Expressed in all the tissues examined; liver, kidney, spleen, heart, lung and brain.1 Publication

Gene expression databases

BgeeiENSMUSG00000034120
CleanExiMM_SFRS2
ExpressionAtlasiQ62093 baseline and differential
GenevisibleiQ62093 MM

Interactioni

Subunit structurei

Interacts with CCNL1 and CCNL2. Interacts with SCAF11. Interacts with ZRSR2/U2AF1-RS2. Interacts with CCDC55 (via C-terminus) (By similarity). In vitro, self-associates and binds SRSF1/SFRS1 (ASF/SF2), SNRNP70 and U2AF1 but not U2AF2. Binds SREK1/SFRS12. Interacts with BRDT.By similarity1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi203188, 5 interactors
DIPiDIP-44831N
IntActiQ62093, 10 interactors
MINTiQ62093
STRINGi10090.ENSMUSP00000090059

Structurei

3D structure databases

ProteinModelPortaliQ62093
SMRiQ62093
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini14 – 92RRMPROSITE-ProRule annotationAdd BLAST79

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi111 – 116Gly-rich (hinge region)6
Compositional biasi117 – 221Arg/Ser-rich (RS domain)Add BLAST105

Sequence similaritiesi

Belongs to the splicing factor SR family.Curated

Phylogenomic databases

eggNOGiKOG4207 Eukaryota
ENOG4111NJ8 LUCA
GeneTreeiENSGT00700000104403
HOVERGENiHBG107480
InParanoidiQ62093
KOiK12891
OMAiVEFRSQR
OrthoDBiEOG091G13K0
TreeFamiTF106262

Family and domain databases

Gene3Di3.30.70.330, 1 hit
InterProiView protein in InterPro
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR000504 RRM_dom
IPR003954 RRM_dom_euk
IPR034893 SRSF2-like
PANTHERiPTHR23147:SF16 PTHR23147:SF16, 1 hit
PfamiView protein in Pfam
PF00076 RRM_1, 1 hit
SMARTiView protein in SMART
SM00360 RRM, 1 hit
SM00361 RRM_1, 1 hit
SUPFAMiSSF54928 SSF54928, 1 hit
PROSITEiView protein in PROSITE
PS50102 RRM, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q62093-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSYGRPPPDV EGMTSLKVDN LTYRTSPDTL RRVFEKYGRV GDVYIPRDRY
60 70 80 90 100
TKESRGFAFV RFHDKRDAED AMDAMDGAVL DGRELRVQMA RYGRPPDSHH
110 120 130 140 150
SRRGPPPRRY GGGGYGRRSR SPRRRRRSRS RSRSRSRSRS RSRYSRSKSR
160 170 180 190 200
SRTRSRSRST SKSRSARRSK SKSSSVSRSR SRSRSRSRSR SPPPVSKRES
210 220
KSRSRSKSPP KSPEEEGAVS S
Length:221
Mass (Da):25,476
Last modified:January 23, 2007 - v4
Checksum:i68121AC4D35714FA
GO

Sequence cautioni

The sequence AAA64595 differs from that shown. Reason: Frameshift at positions 56, 125 and 141.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti66 – 67RD → PH in AAA64595 (PubMed:7527040).Curated2
Sequence conflicti89M → L in AAA64595 (PubMed:7527040).Curated1
Sequence conflicti120Missing (PubMed:7527040).Curated1
Sequence conflicti218A → E in AAA64595 (PubMed:7527040).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14648 mRNA Translation: AAA64595.1 Frameshift.
AF077858 mRNA Translation: AAC71000.1
AK086103 mRNA Translation: BAC39610.1
AK088042 mRNA Translation: BAC40111.1
AK165670 mRNA Translation: BAE38330.1
BC005493 mRNA Translation: AAH05493.1
X98511 Genomic DNA Translation: CAA67134.1
CCDSiCCDS25680.1
PIRiA55335
RefSeqiNP_035488.1, NM_011358.2
XP_011247137.1, XM_011248835.2
UniGeneiMm.21841
Mm.429312
Mm.469331

Genome annotation databases

EnsembliENSMUST00000092404; ENSMUSP00000090059; ENSMUSG00000034120
ENSMUST00000136914; ENSMUSP00000120086; ENSMUSG00000034120
ENSMUST00000190993; ENSMUSP00000140016; ENSMUSG00000034120
GeneIDi20382
KEGGimmu:20382
UCSCiuc007mmn.2 mouse

Similar proteinsi

Entry informationi

Entry nameiSRSF2_MOUSE
AccessioniPrimary (citable) accession number: Q62093
Secondary accession number(s): Q542L3, Q60701
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 23, 2007
Last modified: May 23, 2018
This is version 168 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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