Mitogen-activated protein kinase kinase kinase 7

Contribute

Send feedback

Read comments (0) or add your own

Reviewed, UniProtKB/Swiss-Prot Q62073 (M3K7_MOUSE)

Last modified October 13, 2009. Version 88. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data |

Customize display

text xml rdf/xml gff fasta

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Hide | Top

Names and origin

Protein names

Recommended name:
    Mitogen-activated protein kinase kinase kinase 7
    EC=2.7.11.25
Alternative name(s):
    Transforming growth factor-beta-activated kinase 1
      Short name=TGF-beta-activated kinase 1

Gene names

Name:	Map3k7
Synonyms:	Tak1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Hide | Top

Protein attributes

Sequence length	579 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

Hide | Top

General annotation (Comments)

Function	Component of a protein kinase signal transduction cascade. Mediator of TGF-beta signal transduction. Stimulates NF-kappa-B activation and the p38 MAPK pathway. Ref.1
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Cofactor	Magnesium.
Subunit structure	Binds both upstream activators and downstream substrates in multimolecular complexes. Interacts with MAP3K7IP1 and MAP3K7IP2 By similarity. Interacts with PPM1L. Interaction with PP2A and PPP6C leads to its' repressed activity By similarity.
Post-translational modification	Association with MAP3K7IP1 promotes autophosphorylation and subsequent activation. Dephosphorylation at Thr-187 by PP2A and PPP6C leads to inactivation By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily. Contains 1 protein kinase domain.

Hide | Top

Ontologies

Keywords
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	I-kappaB phosphorylation Inferred from genetic interaction. Source: MGI MAPKKK cascade Ref.2 Inferred from direct assay. Source: MGI angiogenesis Inferred from mutant phenotype. Source: MGI negative regulation of apoptosis Inferred from genetic interaction. Source: MGI neural tube formation Inferred from mutant phenotype. Source: MGI positive regulation of I-kappaB kinase/NF-kappaB cascade Inferred from genetic interaction. Source: MGI positive regulation of JNK cascade Inferred from genetic interaction. Source: MGI transforming growth factor beta receptor signaling pathway Inferred from mutant phenotype. Source: MGI
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW MAP kinase kinase activity Inferred from direct assay. Source: MGI MAP kinase kinase kinase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Hide | Top

Binary interactions

With	Entry	#Exp.	IntAct	Notes
MAP3K7IP2	Q9NYJ8	1	EBI-1775345,EBI-358708	From a different organism.
Map3k7ip2	Q99K90	4	EBI-1775345,EBI-1775124
MAP3K7IP3	Q8N5C8	1	EBI-1775345,EBI-359964	From a different organism.
Mapk8ip1	Q9WVI9-2	1	EBI-1775345,EBI-288464
VRK2	Q86Y07-1	2	EBI-1775345,EBI-1207633	From a different organism.

Hide | Top

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 579

579

Mitogen-activated protein kinase kinase kinase 7

PRO_0000086253

Regions

Domain

36 – 291

256

Protein kinase

Nucleotide binding

42 – 50

ATP By similarity

Compositional bias

8 – 16

Poly-Ser

Sites

Active site

156

Proton acceptor By similarity

Binding site

ATP By similarity

Amino acid modifications

Modified residue

184

Phosphothreonine; by autocatalysis By similarity

Modified residue

187

Phosphothreonine; by autocatalysis By similarity

Modified residue

192

Phosphoserine; by autocatalysis By similarity

Modified residue

389

Phosphoserine Ref.5

Modified residue

412

Phosphoserine Ref.5 Ref.3 Ref.4

Hide | Top

Sequences

Sequence

Length

Mass (Da)

Tools

Q62073-1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 97C8F6F3C8E283EE
Show »

FASTA

579

64,228

        10         20         30         40         50         60 
MSTASAASSS SSSSASEMIE APSQVLNFEE IDYKEIEVEE VVGRGAFGVV CKAKWRAKDV 

        70         80         90        100        110        120 
AIKQIESESE RKAFIVELRQ LSRVNHPNIV KLYGACLNPV CLVMEYAEGG SLYNVLHGAE 

       130        140        150        160        170        180 
PLPYYTAAHA MSWCLQCSQG VAYLHSMQPK ALIHRDLKPP NLLLVAGGTV LKICDFGTAC 

       190        200        210        220        230        240 
DIQTHMTNNK GSAAWMAPEV FEGSNYSEKC DVFSWGIILW EVITRRKPFD EIGGPAFRIM 

       250        260        270        280        290        300 
WAVHNGTRPP LIKNLPKPIE SLMTRCWSKD PSQRPSMEEI VKIMTHLMRY FPGADEPLQY 

       310        320        330        340        350        360 
PCQYSDEGQS NSATSTGSFM DIASTNTSNK SDTNMEQVPA TNDTIKRLES KLLKNQAKQQ 

       370        380        390        400        410        420 
SESGRLSLGA SRGSSVESLP PTSEGKRMSA DMSEIEARIV ATAGNGQPRR RSIQDLTVTG 

       430        440        450        460        470        480 
TEPGQVSSRS SSPSVRMITT SGPTSEKPAR SHPWTPDDST DTNGSDNSIP MAYLTLDHQL 

       490        500        510        520        530        540 
QPLAPCPNSK ESMAVFEQHC KMAQEYMKVQ TEIALLLQRK QELVAELDQD EKDQQNTSRL 

       550        560        570 
VQEHKKLLDE NKSLSTYYQQ CKKQLEVIRS QQQKRQGTS

« Hide

Hide | Top

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a member of the MAPKKK family as a potential mediator of TGF-beta signal transduction." Yamaguchi K., Shirakabe K., Shibuya H., Irie K., Ohishi I., Ueno N., Taniguchi T., Nishida E., Matsumoto K. Science 270:2008-2011(1995) [PubMed: 8533096] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
[2]	"Regulation of the interleukin-1-induced signaling pathways by a novel member of the protein phosphatase 2C family (PP2Cepsilon)." Li M.G., Katsura K., Nomiyama H., Komaki K., Ninomiya-Tsuji J., Matsumoto K., Kobayashi T., Tamura S. J. Biol. Chem. 278:12013-12021(2003) [PubMed: 12556533] [Abstract] Cited for: INTERACTION WITH PPM1L.
[3]	"Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations." Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M. Mol. Cell. Proteomics 6:283-293(2007) [PubMed: 17114649] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-412, MASS SPECTROMETRY. Tissue: Brain cortex.
[4]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed: 17242355] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-412, MASS SPECTROMETRY. Tissue: Liver.
[5]	"The phagosomal proteome in interferon-gamma-activated macrophages." Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P. Immunity 30:143-154(2009) [PubMed: 19144319] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-389 AND SER-412, MASS SPECTROMETRY. Tissue: Macrophage.
+	Additional computationally mapped references.

Hide | Top

Cross-references

Sequence databases
	D76446 mRNA. Translation: BAA11184.1.
IPI	IPI00121080.
RefSeq	NP_766276.1.
UniGene	Mm.258589
3D structure databases
HSSP	HSSP built from PDB template 1IAS based on UniProtKB P36897.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q62073. 5 interactions.
STRING	Q62073.
PTM databases
PhosphoSite	Q62073.
Proteomic databases
PRIDE	Q62073.
Genome annotation databases
Ensembl	ENSMUST00000037607; ENSMUSP00000040307; ENSMUSG00000028284; Mus musculus. [Genome view] ENSMUST00000080933; ENSMUSP00000079734; ENSMUSG00000028284; Mus musculus. [Genome view]
GeneID	26409.
KEGG	mmu:26409.
UCSC	uc008sep.1. mouse.
Organism-specific databases
CTD	26409.
MGI	MGI:1346877. Map3k7.
Phylogenomic databases
HOVERGEN	Q62073.
Enzyme and pathway databases
BRENDA	2.7.11.25. 244.
Gene expression databases
ArrayExpress	Q62073.
Bgee	Q62073.
CleanEx	MM_MAP3K7.
Genevestigator	Q62073.
GermOnline	ENSMUSG00000028284. Mus musculus.
Family and domain databases
InterPro	IPR015786. MAPKKK-related. IPR017421. MAPKKK7. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR001245. Tyr_pkinase. [Graphical view]
PANTHER	PTHR23257:SF92. MAPKKK-related. 1 hit.
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
PIRSF	PIRSF038168. MAPKKK7. 1 hit.
PRINTS	PR00109. TYRKINASE.
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	304393.
SOURCE	Search...

Hide | Top

Entry information

Entry name

M3K7_MOUSE

Accession

Primary (citable) accession number: Q62073

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	November 1, 1996
Last modified:	October 13, 2009
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Hide | Top

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents