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Protein

Astrocytic phosphoprotein PEA-15

Gene

Pea15

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Blocks Ras-mediated inhibition of integrin activation and modulates the ERK MAP kinase cascade. Inhibits RPS6KA3 activities by retaining it in the cytoplasm. Inhibits both TNFRSF6- and TNFRSF1A-mediated CASP8 activity and apoptosis. Regulates glucose transport by controlling both the content of SLC2A1 glucose transporters on the plasma membrane and the insulin-dependent trafficking of SLC2A4 from the cell interior to the surface (By similarity).By similarity1 Publication

GO - Molecular functioni

  • protein kinase C binding Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Apoptosis, Sugar transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-112409. RAF-independent MAPK1/3 activation.
R-MMU-5673001. RAF/MAP kinase cascade.

Names & Taxonomyi

Protein namesi
Recommended name:
Astrocytic phosphoprotein PEA-15
Alternative name(s):
15 kDa phosphoprotein enriched in astrocytes
Gene namesi
Name:Pea15
Synonyms:Pea15a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:104799. Pea15a.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • microtubule associated complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi104S → A: Abolishes inhibitory effect on FAS-mediated apoptosis. Does not change effect on TNFRSF1A-mediated apoptosis. 1 Publication1
Mutagenesisi116S → A: Abolishes inhibitory effect on FAS-mediated apoptosis. Does not change effect on TNFRSF1A-mediated apoptosis. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001912831 – 130Astrocytic phosphoprotein PEA-15Add BLAST130

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei61PhosphoserineBy similarity1
Modified residuei90PhosphoserineCombined sources1
Modified residuei104Phosphoserine; by PKCCombined sources1 Publication1
Modified residuei116Phosphoserine; by CaMK2Combined sources1 Publication1

Post-translational modificationi

Phosphorylated by protein kinase C and calcium-calmodulin-dependent protein kinase. These phosphorylation events are modulated by neurotransmitters or hormones.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ62048.
PaxDbiQ62048.
PeptideAtlasiQ62048.
PRIDEiQ62048.

PTM databases

iPTMnetiQ62048.
PhosphoSitePlusiQ62048.

Expressioni

Tissue specificityi

Predominantly expressed in the brain. Low levels in some peripheral organs.

Gene expression databases

BgeeiENSMUSG00000013698.
CleanExiMM_PEA15A.
ExpressionAtlasiQ62048. baseline and differential.
GenevisibleiQ62048. MM.

Interactioni

Subunit structurei

Binds RPS6KA3, MAPK3 and MAPK1. Interacts with CASP8 and FADD (By similarity). Transient interaction with PLD1 and PLD2.By similarity1 Publication

GO - Molecular functioni

  • protein kinase C binding Source: MGI

Protein-protein interaction databases

BioGridi202103. 2 interactors.
DIPiDIP-60009N.
IntActiQ62048. 1 interactor.
MINTiMINT-4106963.
STRINGi10090.ENSMUSP00000013842.

Structurei

Secondary structure

1130
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi2 – 14Combined sources13
Helixi17 – 26Combined sources10
Turni27 – 30Combined sources4
Helixi33 – 37Combined sources5
Helixi42 – 51Combined sources10
Turni52 – 54Combined sources3
Helixi61 – 69Combined sources9
Helixi73 – 89Combined sources17

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2LS7NMR-A1-92[»]
ProteinModelPortaliQ62048.
SMRiQ62048.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 81DEDPROSITE-ProRule annotationAdd BLAST79

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni98 – 107Microtubule-bindingSequence analysis10
Regioni122 – 129Microtubule-bindingSequence analysis8

Sequence similaritiesi

Contains 1 DED (death effector) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3573. Eukaryota.
ENOG410ZQIE. LUCA.
GeneTreeiENSGT00390000000230.
HOGENOMiHOG000049048.
HOVERGENiHBG053557.
InParanoidiQ62048.
OMAiSPVMAEY.
OrthoDBiEOG091G0TNX.
PhylomeDBiQ62048.
TreeFamiTF332405.

Family and domain databases

CDDicd08338. DED_PEA15. 1 hit.
Gene3Di1.10.533.10. 1 hit.
InterProiIPR011029. DEATH-like_dom.
IPR001875. DED_dom.
IPR029546. PEA15.
[Graphical view]
PANTHERiPTHR15094:SF0. PTHR15094:SF0. 1 hit.
PfamiPF01335. DED. 1 hit.
[Graphical view]
SMARTiSM00031. DED. 1 hit.
[Graphical view]
SUPFAMiSSF47986. SSF47986. 1 hit.
PROSITEiPS50168. DED. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q62048-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAEYGTLLQD LTNNITLEDL EQLKSACKED IPSEKSEEIT TGSAWFSFLE
60 70 80 90 100
SHNKLDKDNL SYIEHIFEIS RRPDLLTMVV DYRTRVLKIS EEEELDTKLT
110 120 130
RIPSAKKYKD IIRQPSEEEI IKLAPPPKKA
Length:130
Mass (Da):15,054
Last modified:November 1, 1996 - v1
Checksum:i780F93A40B2834A8
GO
Isoform 2 (identifier: Q62048-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     36-57: Missing.
     58-58: D → N

Show »
Length:108
Mass (Da):12,544
Checksum:iBCF0527AE97E42AF
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_00773636 – 57Missing in isoform 2. 1 PublicationAdd BLAST22
Alternative sequenceiVSP_00773758D → N in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X86694 Genomic DNA. Translation: CAA60387.1.
AK089070 mRNA. Translation: BAC40734.1.
AK153141 mRNA. Translation: BAE31752.1.
AK153493 mRNA. Translation: BAE32041.1.
AK161635 mRNA. Translation: BAE36504.1.
AK161977 mRNA. Translation: BAE36662.1.
BC038282 mRNA. Translation: AAH38282.1.
CCDSiCCDS15510.1. [Q62048-1]
PIRiS55385.
RefSeqiNP_001316798.1. NM_001329869.1.
NP_001316800.1. NM_001329871.1.
NP_035193.1. NM_011063.3. [Q62048-1]
XP_006496765.1. XM_006496702.1. [Q62048-1]
UniGeneiMm.544.

Genome annotation databases

EnsembliENSMUST00000013842; ENSMUSP00000013842; ENSMUSG00000013698. [Q62048-1]
ENSMUST00000111247; ENSMUSP00000106878; ENSMUSG00000013698. [Q62048-2]
GeneIDi18611.
KEGGimmu:18611.
UCSCiuc007dpy.1. mouse. [Q62048-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X86694 Genomic DNA. Translation: CAA60387.1.
AK089070 mRNA. Translation: BAC40734.1.
AK153141 mRNA. Translation: BAE31752.1.
AK153493 mRNA. Translation: BAE32041.1.
AK161635 mRNA. Translation: BAE36504.1.
AK161977 mRNA. Translation: BAE36662.1.
BC038282 mRNA. Translation: AAH38282.1.
CCDSiCCDS15510.1. [Q62048-1]
PIRiS55385.
RefSeqiNP_001316798.1. NM_001329869.1.
NP_001316800.1. NM_001329871.1.
NP_035193.1. NM_011063.3. [Q62048-1]
XP_006496765.1. XM_006496702.1. [Q62048-1]
UniGeneiMm.544.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2LS7NMR-A1-92[»]
ProteinModelPortaliQ62048.
SMRiQ62048.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202103. 2 interactors.
DIPiDIP-60009N.
IntActiQ62048. 1 interactor.
MINTiMINT-4106963.
STRINGi10090.ENSMUSP00000013842.

PTM databases

iPTMnetiQ62048.
PhosphoSitePlusiQ62048.

Proteomic databases

EPDiQ62048.
PaxDbiQ62048.
PeptideAtlasiQ62048.
PRIDEiQ62048.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000013842; ENSMUSP00000013842; ENSMUSG00000013698. [Q62048-1]
ENSMUST00000111247; ENSMUSP00000106878; ENSMUSG00000013698. [Q62048-2]
GeneIDi18611.
KEGGimmu:18611.
UCSCiuc007dpy.1. mouse. [Q62048-1]

Organism-specific databases

CTDi18611.
MGIiMGI:104799. Pea15a.

Phylogenomic databases

eggNOGiKOG3573. Eukaryota.
ENOG410ZQIE. LUCA.
GeneTreeiENSGT00390000000230.
HOGENOMiHOG000049048.
HOVERGENiHBG053557.
InParanoidiQ62048.
OMAiSPVMAEY.
OrthoDBiEOG091G0TNX.
PhylomeDBiQ62048.
TreeFamiTF332405.

Enzyme and pathway databases

ReactomeiR-MMU-112409. RAF-independent MAPK1/3 activation.
R-MMU-5673001. RAF/MAP kinase cascade.

Miscellaneous databases

PROiQ62048.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000013698.
CleanExiMM_PEA15A.
ExpressionAtlasiQ62048. baseline and differential.
GenevisibleiQ62048. MM.

Family and domain databases

CDDicd08338. DED_PEA15. 1 hit.
Gene3Di1.10.533.10. 1 hit.
InterProiIPR011029. DEATH-like_dom.
IPR001875. DED_dom.
IPR029546. PEA15.
[Graphical view]
PANTHERiPTHR15094:SF0. PTHR15094:SF0. 1 hit.
PfamiPF01335. DED. 1 hit.
[Graphical view]
SMARTiSM00031. DED. 1 hit.
[Graphical view]
SUPFAMiSSF47986. SSF47986. 1 hit.
PROSITEiPS50168. DED. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPEA15_MOUSE
AccessioniPrimary (citable) accession number: Q62048
Secondary accession number(s): Q3U5N7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 138 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Increases PLD1 and PLD2 levels, possibly by stabilizing the protein.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.