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Protein

Centrosomal protein of 131 kDa

Gene

Cep131

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of centriolar satellites contributing to the building of a complex and dynamic network required to regulate cilia/flagellum formation. In proliferating cells, MIB1-mediated ubiquitination induces its sequestration within centriolar satellites, precluding untimely cilia formation initiation. In contrast, during normal and ultraviolet or heat shock cellular stress-induced ciliogenesis, its non-ubiquitinated form is rapidly displaced from centriolar satellites and recruited to centrosome/basal bodies in a microtubule- and p38 MAPK-dependent manner. Acts also as a negative regulator of BBSome ciliary trafficking (By similarity). Plays a role in sperm flagellar formation; may be involved in the regulation of intraflagellar transport (IFT) and/or intramanchette (IMT) trafficking, which are important for axoneme extension and/or cargo delivery to the nascent sperm tail (PubMed:24415959). Required for optimal cell proliferation and cell cycle progression; may play a role in the regulation of genome stability and centriole duplication in non-ciliogenic cells (By similarity). Involved in centriole duplication (PubMed:26297806). Required for CEP152, WDR62 and CEP63 centrosomal localization and promotes the centrosomal localization of CDK2 (By similarity).By similarity3 Publications

GO - Molecular functioni

  • protein complex binding Source: UniProtKB
  • protein homodimerization activity Source: UniProtKB

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • cilium morphogenesis Source: GO_Central
  • intraciliary transport Source: MGI
  • intraciliary transport involved in cilium morphogenesis Source: UniProtKB
  • multicellular organism development Source: UniProtKB-KW
  • nonmotile primary cilium assembly Source: MGI
  • positive regulation of cell proliferation Source: UniProtKB
  • positive regulation of intracellular protein transport Source: UniProtKB
  • protein localization to centrosome Source: MGI
  • regulation of centrosome duplication Source: UniProtKB
  • spermatid development Source: MGI
  • spermatogenesis Source: UniProtKB
  • sperm axoneme assembly Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Cell cycle, Cilium biogenesis/degradation, Differentiation, Spermatogenesis, Transport

Enzyme and pathway databases

ReactomeiR-MMU-2565942. Regulation of PLK1 Activity at G2/M Transition.
R-MMU-380259. Loss of Nlp from mitotic centrosomes.
R-MMU-380270. Recruitment of mitotic centrosome proteins and complexes.
R-MMU-5620912. Anchoring of the basal body to the plasma membrane.
R-MMU-8854518. AURKA Activation by TPX2.

Names & Taxonomyi

Protein namesi
Recommended name:
Centrosomal protein of 131 kDa
Alternative name(s):
5-azacytidine-induced protein 1
Pre-acrosome localization protein 1
Gene namesi
Name:Cep131
Synonyms:Az1, Azi, Azi1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:107440. Cep131.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cytoplasm, Cytoplasmic vesicle, Cytoskeleton

Pathology & Biotechi

Disruption phenotypei

Females grow normally and are healthy. Males display developing sperm flagella abnormalities resulting in infertility. Post-meiotic defects during spermatogenesis with abnormal morphology of elongating and elongated spermatids, including teratozoospermia, premature apoptosis, but without increase in DNA damage. Sperm are immotile with shortened and morphologically abnormal flagella, and altered intraflagellar transport (IFT) and/or intramanchette (IMT) trafficking. Displays normal postnatal multiciliated airway epithelium. Lacks retinal degeneration and kidney cysts formations.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10601060Centrosomal protein of 131 kDaPRO_0000064782Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei14 – 141PhosphoserineCombined sources
Modified residuei35 – 351PhosphoserineBy similarity
Modified residuei47 – 471PhosphoserineCombined sources
Modified residuei78 – 781PhosphoserineBy similarity
Modified residuei89 – 891PhosphoserineBy similarity
Modified residuei105 – 1051PhosphoserineBy similarity
Modified residuei114 – 1141PhosphoserineBy similarity
Modified residuei146 – 1461PhosphoserineBy similarity
Modified residuei150 – 1501PhosphoserineBy similarity
Modified residuei473 – 4731PhosphothreonineCombined sources
Modified residuei481 – 4811PhosphoserineCombined sources

Post-translational modificationi

Ubiquitinated. Undergoes monoubiquitination catalyzed by the E3 ubiquitin-protein ligase MIB1 in proliferating cells, preventing cilia formation. Monoubiquitination by MIB1 is inhibited in response to cellular stress, such as ultraviolet light (UV) radiation or heat shock, resulting in ciliogenesis restoration (By similarity).By similarity
MAPKAPK2-dependent phosphorylation at Ser-47 and Ser-78 occurs in response to cellular stress such as exposure to ultraviolet irradiation and promotes binding to 14-3-3 proteins which leads to cytoplasmic sequestration of CEP131 and blocks formation of new centriolar satellites.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ62036.
MaxQBiQ62036.
PaxDbiQ62036.
PRIDEiQ62036.

PTM databases

iPTMnetiQ62036.
PhosphoSiteiQ62036.

Expressioni

Tissue specificityi

Localized to the pre-acrosome region of round and elongated spermatids in testis but also present in ovary, brain and adipose tissue.1 Publication

Developmental stagei

Widely expressed during development, with strong expression in tissues with high levels of cilia-dependent developmental signaling such as the limbs, eyes, somite derivatives and brain. Significant amounts are found in the testis of 16 day old mice, at a late stage of pachytene spermatocytes when meiosis occurs. The level increases thereafter.1 Publication

Inductioni

By 5-azacytidine.

Gene expression databases

BgeeiQ62036.
CleanExiMM_AZI1.
ExpressionAtlasiQ62036. baseline and differential.
GenevisibleiQ62036. MM.

Interactioni

Subunit structurei

Self-associates (By similarity). Associates with the centriolar satellite BBSome protein complex (PubMed:24550735) Interacts with BBS4; the interaction limits BBS4 availability for association with the BBSome complex, and hence negatively regulates ciliary localization of the BBSome complex. Interacts with MIB1. Interacts with PCM1; the interaction increases in response to ultraviolet light (UV) radiation. Associates with microtubule; association to microtubule is reduced in response to cellular stress, such as UV stimulation, in a process that requires p38 MAP kinase signaling. Interacts with CEP290, DCTN1, MAP1LC3B, PCNT, PCM1 and CEP152 (By similarity). Interacts with 14-3-3 proteins following UV-induced phosphorylation by MAPKAPK2; this inhibits formation of novel centriolar satellites (By similarity).By similarity1 Publication

GO - Molecular functioni

  • protein complex binding Source: UniProtKB
  • protein homodimerization activity Source: UniProtKB

Protein-protein interaction databases

BioGridi198290. 44 interactions.
IntActiQ62036. 46 interactions.
MINTiMINT-4088912.
STRINGi10090.ENSMUSP00000101834.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini263 – 28321IQAdd
BLAST

Sequence similaritiesi

Belongs to the CEP131 family.Curated
Contains 1 IQ domain.Curated

Phylogenomic databases

eggNOGiENOG410IEUN. Eukaryota.
ENOG410YVPU. LUCA.
GeneTreeiENSGT00390000001758.
HOGENOMiHOG000293266.
HOVERGENiHBG024371.
InParanoidiQ62036.
KOiK16540.
OMAiEKGATWN.

Family and domain databases

InterProiIPR030465. CEP131.
[Graphical view]
PANTHERiPTHR31540:SF1. PTHR31540:SF1. 1 hit.

Sequencei

Sequence statusi: Complete.

Q62036-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKGSRTITAT PEGSPEAVDL SLIGLPPPMS QRPGSASATR SIFRSMSVAT
60 70 80 90 100
GSEPRKKALE ATGPGGPRAI NNLRRSNSTT QVNQSWTGSP RPAEPTDFLM
110 120 130 140 150
LFEGSTSGRR RVASLSKASS EKEATWNVLD EQPRGLALPA SAQSPSTLDS
160 170 180 190 200
ALGPRRKECP LAPSFTANNR SNKGAVGNCV TTMVHNHYAS SKMVSPPKSS
210 220 230 240 250
NQTAPSLNNI VKAAAREGGE GSDLGKPRKN LSSASQSARG TTGLLRRREV
260 270 280 290 300
TEEEAERFIH QVNQAAVTIQ RWYRCQVQRR RAGAAALEHL LASKREGQRQ
310 320 330 340 350
RLGGGNLLEL HRQEEAARKK AREEKARQAR QAAIQVLQQK RAQKASEAEH
360 370 380 390 400
RRPKDRPETR APEQPRPMQE PGCVTHPKAN NAGASIYPTG PADPCPPASE
410 420 430 440 450
SSPEQWQSPE DKPQDIHSQG EARQDLAVSG SSRGKARARA TLDDLLDTLK
460 470 480 490 500
LLEEEPEPLP HPKAYHKDRY AWTDEEEDAN SLTADNLEKF GKLSAAPGPP
510 520 530 540 550
DDGTLLSEAK LQSIMTFLDE MEKSGQERPA PWRESLVLEA GSGSEGSTSV
560 570 580 590 600
MRLKLELEEK KQAMALLQRA LAQQRDLTVR RVKETEKELT RQLRQQKEQY
610 620 630 640 650
EATIQRHLSF IDQLIEDKKV LSEKCEAVVA ELKHGDQRCR ERVAQMQEQH
660 670 680 690 700
ELEIKKLKEL MSATEKIRRE KWINEKTKKI KEITVRGLEP EIQKLIAKHK
710 720 730 740 750
QEVRRLRGLH EAELQQREEQ AAQRHLRQAE ELRQHLDRER EVLGQQERER
760 770 780 790 800
AQQRFEQHLE QEQRALEQQR RRLYNEVAEE KERLGQQAAR QRAELEELRQ
810 820 830 840 850
QLEESSAALT RALRAEFERS REEQERRHQM ELKALKDQLE AERQAWVASC
860 870 880 890 900
AKKEEAWLLT RERELKEEIR KGRDQEIELV IHRLEADMTL AKEESERAAE
910 920 930 940 950
SRVKRVRDKY ETELSELEQS ERKLQERCSE LKGRLGEAEG EKERLQSLVR
960 970 980 990 1000
QKEKELEDLR AVNTQMCSER ASLAQVVRQE FAEQLAASQE ETQRVKVELA
1010 1020 1030 1040 1050
ELQARQQVEL DEVHRRVKTA LARKEAAVNS LRKQHEAAVK RADHLEELLE
1060
QHKGSSLSSK
Length:1,060
Mass (Da):120,313
Last modified:July 27, 2011 - v2
Checksum:iFAAC3F922EB83A96
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti336 – 3361V → E in BAA07868 (PubMed:8529672).Curated
Sequence conflicti336 – 3361V → E in BAA19002 (PubMed:9070930).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D43921 mRNA. Translation: BAA07868.1.
D88509 Genomic DNA. Translation: BAA19002.1.
AL807824 Genomic DNA. Translation: CAM22596.1.
PIRiS63993.
RefSeqiNP_033864.3. NM_009734.3.
XP_006532124.1. XM_006532061.2.
UniGeneiMm.2556.

Genome annotation databases

EnsembliENSMUST00000106229; ENSMUSP00000101836; ENSMUSG00000039781.
GeneIDi12009.
KEGGimmu:12009.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D43921 mRNA. Translation: BAA07868.1.
D88509 Genomic DNA. Translation: BAA19002.1.
AL807824 Genomic DNA. Translation: CAM22596.1.
PIRiS63993.
RefSeqiNP_033864.3. NM_009734.3.
XP_006532124.1. XM_006532061.2.
UniGeneiMm.2556.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198290. 44 interactions.
IntActiQ62036. 46 interactions.
MINTiMINT-4088912.
STRINGi10090.ENSMUSP00000101834.

PTM databases

iPTMnetiQ62036.
PhosphoSiteiQ62036.

Proteomic databases

EPDiQ62036.
MaxQBiQ62036.
PaxDbiQ62036.
PRIDEiQ62036.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000106229; ENSMUSP00000101836; ENSMUSG00000039781.
GeneIDi12009.
KEGGimmu:12009.

Organism-specific databases

CTDi22994.
MGIiMGI:107440. Cep131.

Phylogenomic databases

eggNOGiENOG410IEUN. Eukaryota.
ENOG410YVPU. LUCA.
GeneTreeiENSGT00390000001758.
HOGENOMiHOG000293266.
HOVERGENiHBG024371.
InParanoidiQ62036.
KOiK16540.
OMAiEKGATWN.

Enzyme and pathway databases

ReactomeiR-MMU-2565942. Regulation of PLK1 Activity at G2/M Transition.
R-MMU-380259. Loss of Nlp from mitotic centrosomes.
R-MMU-380270. Recruitment of mitotic centrosome proteins and complexes.
R-MMU-5620912. Anchoring of the basal body to the plasma membrane.
R-MMU-8854518. AURKA Activation by TPX2.

Miscellaneous databases

PROiQ62036.
SOURCEiSearch...

Gene expression databases

BgeeiQ62036.
CleanExiMM_AZI1.
ExpressionAtlasiQ62036. baseline and differential.
GenevisibleiQ62036. MM.

Family and domain databases

InterProiIPR030465. CEP131.
[Graphical view]
PANTHERiPTHR31540:SF1. PTHR31540:SF1. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation of a novel cDNA that encodes a protein localized to the pre-acrosome region of spermatids."
    Aoto H., Tsuchida J., Nishina Y., Nishimune Y., Asano A., Tajima S.
    Eur. J. Biochem. 234:8-15(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
  2. "Genomic organization of the mouse AZ1 gene that encodes the protein localized to preacrosomes of spermatids."
    Aoto H., Miyake Y., Nakamura M., Tajima S.
    Genomics 40:138-141(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Strain: BALB/cJ.
    Tissue: Liver.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-14; SER-47; THR-473 AND SER-481, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Kidney, Lung, Spleen and Testis.
  6. "Cilia born out of shock and stress."
    Chavali P.L., Gergely F.
    EMBO J. 32:3011-3013(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW, FUNCTION.
  7. "Acute versus chronic loss of mammalian Azi1/Cep131 results in distinct ciliary phenotypes."
    Hall E.A., Keighren M., Ford M.J., Davey T., Jarman A.P., Smith L.B., Jackson I.J., Mill P.
    PLoS Genet. 9:E1003928-E1003928(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN SPERMIOGENESIS, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
  8. "The centriolar satellite protein AZI1 interacts with BBS4 and regulates ciliary trafficking of the BBSome."
    Chamling X., Seo S., Searby C.C., Kim G., Slusarski D.C., Sheffield V.C.
    PLoS Genet. 10:E1004083-E1004083(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: ASSOCIATION WITH THE BBSOME COMPLEX.
  9. "Centriolar satellites assemble centrosomal microcephaly proteins to recruit CDK2 and promote centriole duplication."
    Kodani A., Yu T.W., Johnson J.R., Jayaraman D., Johnson T.L., Al-Gazali L., Sztriha L., Partlow J.N., Kim H., Krup A.L., Dammermann A., Krogan N., Walsh C.A., Reiter J.F.
    Elife 4:0-0(2015) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH CEP152 AND PCM1.

Entry informationi

Entry nameiCP131_MOUSE
AccessioniPrimary (citable) accession number: Q62036
Secondary accession number(s): B1AXJ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: July 27, 2011
Last modified: July 6, 2016
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Transient cell cultured-based knock-down (by RNAi) of CEP131 leads to a reduction in ciliogenesis (PubMed:24415959). However, chronic absence of CEP131 following genetic deletion (knockout) shows that cilia develop and function normally in vivo. This suggests that CEP131 is not essential for ciliogenesis, except for the modified cilia of the developing sperm flagella, and that there is an alternative mechanism to compensate for the lack of CEP131 (PubMed:24415959).1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.