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Protein

Platelet-activating factor receptor

Gene

Ptafr

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Receptor for platelet activating factor, a chemotactic phospholipid mediator that possesses potent inflammatory, smooth-muscle contractile and hypotensive activity. Seems to mediate its action via a G protein that activates a phosphatidylinositol-calcium second messenger system.

GO - Molecular functioni

  • lipopolysaccharide binding Source: MGI
  • lipopolysaccharide receptor activity Source: MGI
  • phospholipid binding Source: MGI
  • platelet activating factor receptor activity Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Keywords - Biological processi

Chemotaxis

Enzyme and pathway databases

ReactomeiR-MMU-373076. Class A/1 (Rhodopsin-like receptors).
R-MMU-416476. G alpha (q) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Platelet-activating factor receptor
Short name:
PAF-R
Short name:
PAFr
Gene namesi
Name:Ptafr
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:106066. Ptafr.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1616ExtracellularSequence analysisAdd
BLAST
Transmembranei17 – 3822Helical; Name=1Sequence analysisAdd
BLAST
Topological domaini39 – 5416CytoplasmicSequence analysisAdd
BLAST
Transmembranei55 – 7420Helical; Name=2Sequence analysisAdd
BLAST
Topological domaini75 – 9117ExtracellularSequence analysisAdd
BLAST
Transmembranei92 – 11322Helical; Name=3Sequence analysisAdd
BLAST
Topological domaini114 – 13320CytoplasmicSequence analysisAdd
BLAST
Transmembranei134 – 15522Helical; Name=4Sequence analysisAdd
BLAST
Topological domaini156 – 18429ExtracellularSequence analysisAdd
BLAST
Transmembranei185 – 20521Helical; Name=5Sequence analysisAdd
BLAST
Topological domaini206 – 23328CytoplasmicSequence analysisAdd
BLAST
Transmembranei234 – 25421Helical; Name=6Sequence analysisAdd
BLAST
Topological domaini255 – 27521ExtracellularSequence analysisAdd
BLAST
Transmembranei276 – 29520Helical; Name=7Sequence analysisAdd
BLAST
Topological domaini296 – 34146CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL3993.
GuidetoPHARMACOLOGYi334.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 341341Platelet-activating factor receptorPRO_0000070094Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi4 – 41N-linked (GlcNAc...)Sequence analysis
Disulfide bondi90 ↔ 173PROSITE-ProRule annotation
Glycosylationi169 – 1691N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ62035.
PaxDbiQ62035.
PRIDEiQ62035.

PTM databases

iPTMnetiQ62035.
PhosphoSiteiQ62035.

Expressioni

Tissue specificityi

Found in a range of organs. Expressed most strongly in spleen, followed by skeletal muscle, lung and small intestine. Expressed at moderate levels in the heart. Expressed at relatively low levels in the brain, liver and kidney.1 Publication

Inductioni

By lipopolysaccharide (LPS).1 Publication

Gene expression databases

BgeeiQ62035.
CleanExiMM_PTAFR.

Interactioni

Subunit structurei

Interacts with ARRB1.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000070925.

Chemistry

BindingDBiQ62035.

Structurei

3D structure databases

ProteinModelPortaliQ62035.
SMRiQ62035. Positions 13-303.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IIY4. Eukaryota.
ENOG410YB0R. LUCA.
GeneTreeiENSGT00640000091213.
HOGENOMiHOG000013041.
HOVERGENiHBG101106.
InParanoidiQ62035.
KOiK04279.
OMAiFLCNLAG.
OrthoDBiEOG7QVM3C.
PhylomeDBiQ62035.
TreeFamiTF350009.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR002282. PAF_rcpt.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR01153. PAFRECEPTOR.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q62035-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEHNGSFRVD SEFRYTLFPI VYSVIFILGV VANGYVLWVF ANLYPSKKLN
60 70 80 90 100
EIKIFMVNLT MADLLFLITL PLWIVYYYNE GDWILPNFLC NVAGCLFFIN
110 120 130 140 150
TYCSVAFLGV ITYNRYQAVA YPIKTAQATT RKRGISLSLI IWVSIVATAS
160 170 180 190 200
YFLATDSTNL VPNKDGSGNI TRCFEHYEPY SVPILVVHVF IAFCFFLVFF
210 220 230 240 250
LIFYCNLVII HTLLTQPMRQ QRKAGVKRRA LWMVCTVLAV FIICFVPHHV
260 270 280 290 300
VQLPWTLAEL GYQTNFHQAI NDAHQITLCL LSTNCVLDPV IYCFLTKKFR
310 320 330 340
KHLSEKFYSM RSSRKCSRAT SDTCTEVIVP ANQTPIVSLK N
Length:341
Mass (Da):39,148
Last modified:November 1, 1996 - v1
Checksum:iCAA8CDDBD8D26897
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti181 – 1822SV → RG in BAC27919 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50872 Genomic DNA. Translation: BAA09468.1.
AK032547 mRNA. Translation: BAC27919.1.
CR931794 Genomic DNA. Translation: CAM25344.1.
CCDSiCCDS38899.1.
PIRiS63666.
RefSeqiNP_001074680.1. NM_001081211.2.
UniGeneiMm.459220.
Mm.89389.

Genome annotation databases

EnsembliENSMUST00000070690; ENSMUSP00000070925; ENSMUSG00000056529.
GeneIDi19204.
KEGGimmu:19204.
UCSCiuc008vbn.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50872 Genomic DNA. Translation: BAA09468.1.
AK032547 mRNA. Translation: BAC27919.1.
CR931794 Genomic DNA. Translation: CAM25344.1.
CCDSiCCDS38899.1.
PIRiS63666.
RefSeqiNP_001074680.1. NM_001081211.2.
UniGeneiMm.459220.
Mm.89389.

3D structure databases

ProteinModelPortaliQ62035.
SMRiQ62035. Positions 13-303.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000070925.

Chemistry

BindingDBiQ62035.
ChEMBLiCHEMBL3993.
GuidetoPHARMACOLOGYi334.

Protein family/group databases

GPCRDBiSearch...

PTM databases

iPTMnetiQ62035.
PhosphoSiteiQ62035.

Proteomic databases

MaxQBiQ62035.
PaxDbiQ62035.
PRIDEiQ62035.

Protocols and materials databases

DNASUi19204.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000070690; ENSMUSP00000070925; ENSMUSG00000056529.
GeneIDi19204.
KEGGimmu:19204.
UCSCiuc008vbn.1. mouse.

Organism-specific databases

CTDi5724.
MGIiMGI:106066. Ptafr.

Phylogenomic databases

eggNOGiENOG410IIY4. Eukaryota.
ENOG410YB0R. LUCA.
GeneTreeiENSGT00640000091213.
HOGENOMiHOG000013041.
HOVERGENiHBG101106.
InParanoidiQ62035.
KOiK04279.
OMAiFLCNLAG.
OrthoDBiEOG7QVM3C.
PhylomeDBiQ62035.
TreeFamiTF350009.

Enzyme and pathway databases

ReactomeiR-MMU-373076. Class A/1 (Rhodopsin-like receptors).
R-MMU-416476. G alpha (q) signalling events.

Miscellaneous databases

ChiTaRSiPtafr. mouse.
PROiQ62035.
SOURCEiSearch...

Gene expression databases

BgeeiQ62035.
CleanExiMM_PTAFR.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR002282. PAF_rcpt.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR01153. PAFRECEPTOR.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A murine platelet-activating factor receptor gene: cloning, chromosomal localization and up-regulation of expression by lipopolysaccharide in peritoneal resident macrophages."
    Ishii S., Matsuda Y., Nakamura M., Waga I., Kume K., Izumi T., Noma M., Shimizu T.
    Biochem. J. 314:671-678(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY, INDUCTION.
    Strain: 129/Sv.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Olfactory bulb.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPTAFR_MOUSE
AccessioniPrimary (citable) accession number: Q62035
Secondary accession number(s): B1B170, Q8C017
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: June 8, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.