Q62011 (PDPN_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Podoplanin

Alternative name(s):
Aggrus
Glycoprotein 38
Short name=Gp38
OTS-8
PA2.26 antigen
T1-alpha
Short name=T1A
Transmembrane glycoprotein E11

Gene names

Name:	Pdpn
Synonyms:	Gp38, Ots8

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	172 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May be involved in cell migration and/or actin cytoskeleton organization. When expressed in keratinocytes, induces changes in cell morphology with transfected cells showing an elongated shape, numerous membrane protrusions, major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion. Required for normal lung cell proliferation and alveolus formation at birth. Induces platelet aggregation. Does not have any effect on folic acid or amino acid transport. Does not function as a water channel or as a regulator of aquaporin-type water channels. Ref.3 Ref.4 Ref.9 Ref.10 Ref.11
Subcellular location	Membrane; Single-pass type I membrane protein. Cell projection › lamellipodium membrane; Single-pass type I membrane protein. Cell projection › filopodium membrane; Single-pass type I membrane protein. Cell projection › microvillus membrane; Single-pass type I membrane protein. Cell projection › ruffle membrane; Single-pass type I membrane protein. Note: Localized to actin-rich microvilli and plasma membrane projections such as filopodia, lamellipodia and ruffles. Ref.3
Tissue specificity	Detected at high levels in lung and brain, at lower levels in kidney, stomach, liver, spleen and esophagus, and not detected in skin and small intestine. Expressed in epithelial cells of choroid plexus, ependyma, glomerulus and alveolus, in mesothelial cells and in endothelia of lymphatic vessels. Also expressed in stromal cells of peripheral lymphoid tissue and thymic epithelial cells. Detected in carcinoma cell lines and cultured fibroblasts. Expressed at higher levels in colon carcinomas than in normal colon tissue. Ref.3 Ref.4 Ref.10
Induction	Down-regulated by treatment with puromycin aminonucleoside. Ref.4
Post-translational modification	Extensively O-glycosylated. Contains sialic acid residues. O-glycosylation is necessary for platelet aggregation activity. Ref.3 Ref.11 The N-terminus is blocked By similarity.
Disruption phenotype	Mice die at birth of respiratory failure due to a low number of attenuated type I cells, narrow and irregular air spaces, and defective formation of alveolar saccules. Ref.9
Sequence similarities	Belongs to the podoplanin family.

Ontologies

Keywords
Biological process	Cell shape
Cellular component	Cell membrane Cell projection Membrane
Domain	Signal Transmembrane Transmembrane helix
Ligand	Sialic acid
Molecular function	Developmental protein
PTM	Glycoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from mutant phenotype. Source: MGI cell morphogenesis Inferred from direct assay Ref.3. Source: UniProtKB cell proliferation Inferred from mutant phenotype. Source: MGI cell-cell adhesion Inferred from mutant phenotype. Source: MGI cellular component movement Inferred from mutant phenotype. Source: MGI lung alveolus development Inferred from mutant phenotype. Source: MGI lymphangiogenesis Inferred from mutant phenotype. Source: MGI prostaglandin metabolic process Inferred from mutant phenotype. Source: MGI regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW signal transduction Inferred from mutant phenotype. Source: MGI visceral serous pericardium development Traceable author statement. Source: DFLAT
Cellular component	filopodium membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW lamellipodium membrane Inferred from electronic annotation. Source: UniProtKB-SubCell microvillus membrane Inferred from electronic annotation. Source: UniProtKB-SubCell ruffle membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 172	150	Podoplanin		PRO_0000021352
Regions
	Topological domain	23 – 141	119	Extracellular Potential
	Transmembrane	142 – 162	21	Helical; Potential
	Topological domain	163 – 172	10	Cytoplasmic
Amino acid modifications
	Glycosylation	37	1	O-linked (GalNAc...) Potential
	Glycosylation	51	1	O-linked (GalNAc...) Potential
	Glycosylation	52	1	O-linked (GalNAc...) Potential
	Glycosylation	53	1	O-linked (GalNAc...) Potential
	Glycosylation	56	1	O-linked (GalNAc...) Potential
	Glycosylation	60	1	N-linked (GlcNAc...) Potential
	Glycosylation	63	1	O-linked (GalNAc...) Potential
	Glycosylation	71	1	O-linked (GalNAc...) Potential
	Glycosylation	77	1	O-linked (GalNAc...) Potential
	Glycosylation	85	1	O-linked (GalNAc...) Potential
	Glycosylation	86	1	O-linked (GalNAc...) Potential
	Glycosylation	87	1	O-linked (GalNAc...) Potential
	Glycosylation	89	1	O-linked (GalNAc...) Potential
	Glycosylation	90	1	O-linked (GalNAc...) Potential
	Glycosylation	100	1	O-linked (GalNAc...) Potential
	Glycosylation	101	1	O-linked (GalNAc...) Potential
	Glycosylation	102	1	O-linked (GalNAc...) Potential
	Glycosylation	107	1	O-linked (GalNAc...) Potential
	Glycosylation	115	1	O-linked (GalNAc...) Potential
Experimental info
	Mutagenesis	34	1	T → A: Eliminates platelet aggregation activity. Ref.10
	Sequence conflict	29 – 31	3	EDD → KNN in AAA37724. Ref.2
	Sequence conflict	38 – 39	2	GD → EN in AAA37724. Ref.2
	Sequence conflict	170 – 172	3	FSP → SRPKELNRTGCSPNTSENKR ASNLPCSPSSSCGGR in AAA39866. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q62011 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: C035ED251918CE6F	FASTA	172	18,233
	10 20 30 40 50 60 MWTVPVLFWV LGSVWFWDSA QGGTIGVNED DIVTPGTGDG MVPPGIEDKI TTTGATGGLN 70 80 90 100 110 120 ESTGKAPLVP TQRERGTKPP LEELSTSATS DHDHREHEST TTVKVVTSHS VDKKTSHPNR 130 140 150 160 170 DNAGDETQTT DKKDGLPVVT LVGIIVGVLL AIGFVGGIFI VVMKKISGRF SP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation of a gene sequence induced later by tumor-promoting 12-O-tetradecanoylphorbol-13-acetate in mouse osteoblastic cells (MC3T3-E1) and expressed constitutively in ras-transformed cells." Nose K., Saito H., Kuroki T. Cell Growth Differ. 1:511-518(1990) [PubMed: 2088477] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Characterization and cloning of a novel glycoprotein expressed by stromal cells in T-dependent areas of peripheral lymphoid tissues." Farr A.G., Berry M.L., Kim A., Nelson A.J., Welch M.P., Aruffo A. J. Exp. Med. 176:1477-1482(1992) [PubMed: 1402691] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c.
[3]	"Identification of PA2.26 antigen as a novel cell-surface mucin-type glycoprotein that induces plasma membrane extensions and increased motility in keratinocytes." Scholl F.G., Gamallo C., Vilaro S., Quintanilla M. J. Cell Sci. 112:4601-4613(1999) [PubMed: 10574709] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 24-30 AND 66-73, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, GLYCOSYLATION.
[4]	"Cloning and expression of the mouse glomerular podoplanin homologue gp38P." Boucherot A., Schreiber R., Pavenstaedt H., Kunzelmann K. Nephrol. Dial. Transplant. 17:978-984(2002) [PubMed: 12032185] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, INDUCTION. Tissue: Kidney.
[5]	"Cloning and characterization studies of mouse E11 gene and its spatial and temporal expression pattern during development." Lu Y., Zhang J., Harris M.A., Harris S.E., Bonewald L., Feng J. Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129/Sv.
[6]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Visual cortex.
[7]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed: 19468303] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6.
[8]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Colon.
[9]	"T1alpha, a lung type I cell differentiation gene, is required for normal lung cell proliferation and alveolus formation at birth." Ramirez M.I., Millien G., Hinds A., Cao Y., Seldin D.C., Williams M.C. Dev. Biol. 256:61-72(2003) [PubMed: 12654292] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[10]	"Molecular identification of aggrus/T1alpha as a platelet aggregation-inducing factor expressed in colorectal tumors." Kato Y., Fujita N., Kunita A., Sato S., Kaneko M., Osawa M., Tsuruo T. J. Biol. Chem. 278:51599-51605(2003) [PubMed: 14522983] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, MUTAGENESIS OF THR-34.
[11]	"Functional sialylated O-glycan to platelet aggregation on Aggrus (T1alpha/Podoplanin) molecules expressed in Chinese hamster ovary cells." Kaneko M., Kato Y., Kunita A., Fujita N., Tsuruo T., Osawa M. J. Biol. Chem. 279:38838-38843(2004) [PubMed: 15231832] [Abstract] Cited for: FUNCTION, GLYCOSYLATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M73748 mRNA. Translation: AAA39866.1.
M96645 mRNA. Translation: AAA37724.1.
AJ250246 mRNA. Translation: CAB58997.1.
AJ297944 mRNA. Translation: CAC16152.1.
AY115493 Genomic DNA. Translation: AAM66761.1.
AK158855 mRNA. Translation: BAE34695.1.
AL611982 Genomic DNA. Translation: CAM21724.1.
BC026551 mRNA. Translation: AAH26551.1.

IPI

IPI00230205.

PIR

A54560.

RefSeq

NP_034459.2. NM_010329.2.

UniGene

Mm.2976.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3IET	X-ray	2.20	Q/X	76-84	[»]

ModBase

Search...

Protein-protein interaction databases

STRING

Q62011.

Proteomic databases

PRIDE

Q62011.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000030317; ENSMUSP00000030317; ENSMUSG00000028583.

GeneID

14726.

KEGG

mmu:14726.

UCSC

uc008vqa.1. mouse.

Organism-specific databases

CTD

10630.

MGI

MGI:103098. Pdpn.

Phylogenomic databases

GeneTree

ENSGT00390000000013.

HOGENOM

HBG283047.

HOVERGEN

HBG080131.

InParanoid

Q62011.

OMA

VDGDTQT.

OrthoDB

EOG47PX73.

Gene expression databases

ArrayExpress

Q62011.

Bgee

Q62011.

CleanEx

MM_PDPN.

Genevestigator

Q62011.

GermOnline

ENSMUSG00000028583. Mus musculus.

Family and domain databases

InterPro

IPR008783. Podoplanin.
[Graphical view]

PANTHER

PTHR16861. Podoplanin. 1 hit.

Pfam

PF05808. Podoplanin. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

286753.

SOURCE

Search...

Entry information

Entry name

PDPN_MOUSE

Accession

Primary (citable) accession number: Q62011
Secondary accession number(s): A2A8J3, Q546R8, Q61612

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	December 14, 2011
This is version 89 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program