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Q62011

- PDPN_MOUSE

UniProt

Q62011 - PDPN_MOUSE

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Protein

Podoplanin

Gene

Pdpn

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

May be involved in cell migration and/or actin cytoskeleton organization. When expressed in keratinocytes, induces changes in cell morphology with transfected cells showing an elongated shape, numerous membrane protrusions, major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion. Required for normal lung cell proliferation and alveolus formation at birth. Induces platelet aggregation. Does not have any effect on folic acid or amino acid transport. Does not function as a water channel or as a regulator of aquaporin-type water channels.5 Publications

GO - Biological processi

  1. cell morphogenesis Source: UniProtKB
  2. cell proliferation Source: MGI
  3. cellular component movement Source: MGI
  4. embryo development Source: Ensembl
  5. inflammatory response Source: Ensembl
  6. lung alveolus development Source: MGI
  7. lung development Source: MGI
  8. lymphangiogenesis Source: MGI
  9. nervous system development Source: Ensembl
  10. positive regulation of cell migration Source: Ensembl
  11. positive regulation of cellular component movement Source: UniProtKB
  12. positive regulation of platelet activation Source: Ensembl
  13. prostaglandin metabolic process Source: MGI
  14. regulation of cell shape Source: UniProtKB-KW
  15. regulation of G1/S transition of mitotic cell cycle Source: MGI
  16. response to hyperoxia Source: Ensembl
  17. signal transduction Source: MGI
  18. single organismal cell-cell adhesion Source: MGI
  19. tube morphogenesis Source: Ensembl
  20. visceral serous pericardium development Source: DFLAT
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Cell shape

Keywords - Ligandi

Sialic acid

Names & Taxonomyi

Protein namesi
Recommended name:
Podoplanin
Alternative name(s):
Aggrus
Glycoprotein 38
Short name:
Gp38
OTS-8
PA2.26 antigen
T1-alpha
Short name:
T1A
Transmembrane glycoprotein E11
Gene namesi
Name:Pdpn
Synonyms:Gp38, Ots8
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 4, UP000000589: Unplaced

Organism-specific databases

MGIiMGI:103098. Pdpn.

Subcellular locationi

Membrane 1 Publication; Single-pass type I membrane protein 1 Publication. Cell projectionlamellipodium membrane 1 Publication; Single-pass type I membrane protein 1 Publication. Cell projectionfilopodium membrane 1 Publication; Single-pass type I membrane protein 1 Publication. Cell projectionmicrovillus membrane 1 Publication; Single-pass type I membrane protein 1 Publication. Cell projectionruffle membrane 1 Publication; Single-pass type I membrane protein 1 Publication
Note: Localized to actin-rich microvilli and plasma membrane projections such as filopodia, lamellipodia and ruffles.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini23 – 141119ExtracellularSequence AnalysisAdd
BLAST
Transmembranei142 – 16221HelicalSequence AnalysisAdd
BLAST
Topological domaini163 – 17210Cytoplasmic

GO - Cellular componenti

  1. apical plasma membrane Source: Ensembl
  2. external side of plasma membrane Source: MGI
  3. filopodium Source: UniProtKB
  4. integral component of membrane Source: UniProtKB-KW
  5. lamellipodium Source: UniProtKB
  6. plasma membrane Source: MGI
  7. ruffle Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice die at birth of respiratory failure due to a low number of attenuated type I cells, narrow and irregular air spaces, and defective formation of alveolar saccules.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi34 – 341T → A: Eliminates platelet aggregation activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence AnalysisAdd
BLAST
Chaini23 – 172150PodoplaninPRO_0000021352Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi37 – 371O-linked (GalNAc...)Sequence Analysis
Glycosylationi51 – 511O-linked (GalNAc...)Sequence Analysis
Glycosylationi52 – 521O-linked (GalNAc...)Sequence Analysis
Glycosylationi53 – 531O-linked (GalNAc...)Sequence Analysis
Glycosylationi56 – 561O-linked (GalNAc...)Sequence Analysis
Glycosylationi60 – 601N-linked (GlcNAc...)Sequence Analysis
Glycosylationi63 – 631O-linked (GalNAc...)Sequence Analysis
Glycosylationi71 – 711O-linked (GalNAc...)Sequence Analysis
Glycosylationi77 – 771O-linked (GalNAc...)Sequence Analysis
Glycosylationi85 – 851O-linked (GalNAc...)Sequence Analysis
Glycosylationi86 – 861O-linked (GalNAc...)Sequence Analysis
Glycosylationi87 – 871O-linked (GalNAc...)Sequence Analysis
Glycosylationi89 – 891O-linked (GalNAc...)Sequence Analysis
Glycosylationi90 – 901O-linked (GalNAc...)Sequence Analysis
Glycosylationi100 – 1001O-linked (GalNAc...)Sequence Analysis
Glycosylationi101 – 1011O-linked (GalNAc...)Sequence Analysis
Glycosylationi102 – 1021O-linked (GalNAc...)Sequence Analysis
Glycosylationi107 – 1071O-linked (GalNAc...)Sequence Analysis
Glycosylationi115 – 1151O-linked (GalNAc...)Sequence Analysis

Post-translational modificationi

Extensively O-glycosylated. Contains sialic acid residues. O-glycosylation is necessary for platelet aggregation activity.2 Publications
The N-terminus is blocked.By similarity

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ62011.
PRIDEiQ62011.

Expressioni

Tissue specificityi

Detected at high levels in lung and brain, at lower levels in kidney, stomach, liver, spleen and esophagus, and not detected in skin and small intestine. Expressed in epithelial cells of choroid plexus, ependyma, glomerulus and alveolus, in mesothelial cells and in endothelia of lymphatic vessels. Also expressed in stromal cells of peripheral lymphoid tissue and thymic epithelial cells. Detected in carcinoma cell lines and cultured fibroblasts. Expressed at higher levels in colon carcinomas than in normal colon tissue.3 Publications

Inductioni

Down-regulated by treatment with puromycin aminonucleoside.1 Publication

Gene expression databases

BgeeiQ62011.
CleanExiMM_PDPN.
ExpressionAtlasiQ62011. baseline and differential.
GenevestigatoriQ62011.

Interactioni

Protein-protein interaction databases

IntActiQ62011. 1 interaction.
STRINGi10090.ENSMUSP00000030317.

Structurei

Secondary structure

1
172
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi81 – 833Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3IETX-ray2.20Q/X76-84[»]
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the podoplanin family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG46902.
GeneTreeiENSGT00390000000013.
HOGENOMiHOG000231122.
HOVERGENiHBG080131.
InParanoidiQ62011.
KOiK16778.
OMAiNMATSHS.
OrthoDBiEOG7DC25R.
PhylomeDBiQ62011.
TreeFamiTF337068.

Family and domain databases

InterProiIPR008783. Podoplanin.
[Graphical view]
PANTHERiPTHR16861. PTHR16861. 1 hit.
PfamiPF05808. Podoplanin. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q62011-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MWTVPVLFWV LGSVWFWDSA QGGTIGVNED DIVTPGTGDG MVPPGIEDKI
60 70 80 90 100
TTTGATGGLN ESTGKAPLVP TQRERGTKPP LEELSTSATS DHDHREHEST
110 120 130 140 150
TTVKVVTSHS VDKKTSHPNR DNAGDETQTT DKKDGLPVVT LVGIIVGVLL
160 170
AIGFVGGIFI VVMKKISGRF SP
Length:172
Mass (Da):18,233
Last modified:November 1, 1997 - v2
Checksum:iC035ED251918CE6F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti29 – 313EDD → KNN in AAA37724. (PubMed:1402691)Curated
Sequence conflicti38 – 392GD → EN in AAA37724. (PubMed:1402691)Curated
Sequence conflicti170 – 1723FSP → SRPKELNRTGCSPNTSENKR ASNLPCSPSSSCGGR in AAA39866. (PubMed:2088477)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M73748 mRNA. Translation: AAA39866.1.
M96645 mRNA. Translation: AAA37724.1.
AJ250246 mRNA. Translation: CAB58997.1.
AJ297944 mRNA. Translation: CAC16152.1.
AY115493 Genomic DNA. Translation: AAM66761.1.
AK158855 mRNA. Translation: BAE34695.1.
AL611982 Genomic DNA. Translation: CAM21724.1.
BC026551 mRNA. Translation: AAH26551.1.
CCDSiCCDS38943.1.
PIRiA54560.
RefSeqiNP_001277751.1. NM_001290822.1.
NP_034459.2. NM_010329.3.
UniGeneiMm.2976.

Genome annotation databases

EnsembliENSMUST00000030317; ENSMUSP00000030317; ENSMUSG00000028583.
ENSMUST00000181754; ENSMUSP00000137969; ENSMUSG00000096951.
GeneIDi14726.
KEGGimmu:14726.
UCSCiuc008vqa.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M73748 mRNA. Translation: AAA39866.1 .
M96645 mRNA. Translation: AAA37724.1 .
AJ250246 mRNA. Translation: CAB58997.1 .
AJ297944 mRNA. Translation: CAC16152.1 .
AY115493 Genomic DNA. Translation: AAM66761.1 .
AK158855 mRNA. Translation: BAE34695.1 .
AL611982 Genomic DNA. Translation: CAM21724.1 .
BC026551 mRNA. Translation: AAH26551.1 .
CCDSi CCDS38943.1.
PIRi A54560.
RefSeqi NP_001277751.1. NM_001290822.1.
NP_034459.2. NM_010329.3.
UniGenei Mm.2976.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3IET X-ray 2.20 Q/X 76-84 [» ]
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q62011. 1 interaction.
STRINGi 10090.ENSMUSP00000030317.

Proteomic databases

MaxQBi Q62011.
PRIDEi Q62011.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000030317 ; ENSMUSP00000030317 ; ENSMUSG00000028583 .
ENSMUST00000181754 ; ENSMUSP00000137969 ; ENSMUSG00000096951 .
GeneIDi 14726.
KEGGi mmu:14726.
UCSCi uc008vqa.1. mouse.

Organism-specific databases

CTDi 10630.
MGIi MGI:103098. Pdpn.

Phylogenomic databases

eggNOGi NOG46902.
GeneTreei ENSGT00390000000013.
HOGENOMi HOG000231122.
HOVERGENi HBG080131.
InParanoidi Q62011.
KOi K16778.
OMAi NMATSHS.
OrthoDBi EOG7DC25R.
PhylomeDBi Q62011.
TreeFami TF337068.

Miscellaneous databases

ChiTaRSi Pdpn. mouse.
NextBioi 286753.
PROi Q62011.
SOURCEi Search...

Gene expression databases

Bgeei Q62011.
CleanExi MM_PDPN.
ExpressionAtlasi Q62011. baseline and differential.
Genevestigatori Q62011.

Family and domain databases

InterProi IPR008783. Podoplanin.
[Graphical view ]
PANTHERi PTHR16861. PTHR16861. 1 hit.
Pfami PF05808. Podoplanin. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation of a gene sequence induced later by tumor-promoting 12-O-tetradecanoylphorbol-13-acetate in mouse osteoblastic cells (MC3T3-E1) and expressed constitutively in ras-transformed cells."
    Nose K., Saito H., Kuroki T.
    Cell Growth Differ. 1:511-518(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Characterization and cloning of a novel glycoprotein expressed by stromal cells in T-dependent areas of peripheral lymphoid tissues."
    Farr A.G., Berry M.L., Kim A., Nelson A.J., Welch M.P., Aruffo A.
    J. Exp. Med. 176:1477-1482(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
  3. "Identification of PA2.26 antigen as a novel cell-surface mucin-type glycoprotein that induces plasma membrane extensions and increased motility in keratinocytes."
    Scholl F.G., Gamallo C., Vilaro S., Quintanilla M.
    J. Cell Sci. 112:4601-4613(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 24-30 AND 66-73, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, GLYCOSYLATION.
  4. "Cloning and expression of the mouse glomerular podoplanin homologue gp38P."
    Boucherot A., Schreiber R., Pavenstaedt H., Kunzelmann K.
    Nephrol. Dial. Transplant. 17:978-984(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, INDUCTION.
    Tissue: Kidney.
  5. "Cloning and characterization studies of mouse E11 gene and its spatial and temporal expression pattern during development."
    Lu Y., Zhang J., Harris M.A., Harris S.E., Bonewald L., Feng J.
    Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129/Sv.
  6. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Visual cortex.
  7. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  8. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Colon.
  9. "T1alpha, a lung type I cell differentiation gene, is required for normal lung cell proliferation and alveolus formation at birth."
    Ramirez M.I., Millien G., Hinds A., Cao Y., Seldin D.C., Williams M.C.
    Dev. Biol. 256:61-72(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  10. "Molecular identification of aggrus/T1alpha as a platelet aggregation-inducing factor expressed in colorectal tumors."
    Kato Y., Fujita N., Kunita A., Sato S., Kaneko M., Osawa M., Tsuruo T.
    J. Biol. Chem. 278:51599-51605(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, MUTAGENESIS OF THR-34.
  11. "Functional sialylated O-glycan to platelet aggregation on Aggrus (T1alpha/Podoplanin) molecules expressed in Chinese hamster ovary cells."
    Kaneko M., Kato Y., Kunita A., Fujita N., Tsuruo T., Osawa M.
    J. Biol. Chem. 279:38838-38843(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, GLYCOSYLATION.
  12. Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF 76-84 IN COMPLEX WITH IMMUNOGLOBULIN.

Entry informationi

Entry nameiPDPN_MOUSE
AccessioniPrimary (citable) accession number: Q62011
Secondary accession number(s): A2A8J3, Q546R8, Q61612
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 26, 2014
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3