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Q62011

- PDPN_MOUSE

UniProt

Q62011 - PDPN_MOUSE

Protein

Podoplanin

Gene

Pdpn

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    May be involved in cell migration and/or actin cytoskeleton organization. When expressed in keratinocytes, induces changes in cell morphology with transfected cells showing an elongated shape, numerous membrane protrusions, major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion. Required for normal lung cell proliferation and alveolus formation at birth. Induces platelet aggregation. Does not have any effect on folic acid or amino acid transport. Does not function as a water channel or as a regulator of aquaporin-type water channels.5 Publications

    GO - Biological processi

    1. cell morphogenesis Source: UniProtKB
    2. cell proliferation Source: MGI
    3. cellular component movement Source: MGI
    4. embryo development Source: Ensembl
    5. inflammatory response Source: Ensembl
    6. lung alveolus development Source: MGI
    7. lung development Source: MGI
    8. lymphangiogenesis Source: MGI
    9. nervous system development Source: Ensembl
    10. positive regulation of cell migration Source: Ensembl
    11. positive regulation of cellular component movement Source: UniProtKB
    12. positive regulation of platelet activation Source: Ensembl
    13. prostaglandin metabolic process Source: MGI
    14. regulation of cell shape Source: UniProtKB-KW
    15. regulation of G1/S transition of mitotic cell cycle Source: MGI
    16. response to hyperoxia Source: Ensembl
    17. signal transduction Source: MGI
    18. single organismal cell-cell adhesion Source: MGI
    19. tube morphogenesis Source: Ensembl
    20. visceral serous pericardium development Source: DFLAT

    Keywords - Molecular functioni

    Developmental protein

    Keywords - Biological processi

    Cell shape

    Keywords - Ligandi

    Sialic acid

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Podoplanin
    Alternative name(s):
    Aggrus
    Glycoprotein 38
    Short name:
    Gp38
    OTS-8
    PA2.26 antigen
    T1-alpha
    Short name:
    T1A
    Transmembrane glycoprotein E11
    Gene namesi
    Name:Pdpn
    Synonyms:Gp38, Ots8
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 4

    Organism-specific databases

    MGIiMGI:103098. Pdpn.

    Subcellular locationi

    Membrane 1 Publication; Single-pass type I membrane protein 1 Publication. Cell projectionlamellipodium membrane 1 Publication; Single-pass type I membrane protein 1 Publication. Cell projectionfilopodium membrane 1 Publication; Single-pass type I membrane protein 1 Publication. Cell projectionmicrovillus membrane 1 Publication; Single-pass type I membrane protein 1 Publication. Cell projectionruffle membrane 1 Publication; Single-pass type I membrane protein 1 Publication
    Note: Localized to actin-rich microvilli and plasma membrane projections such as filopodia, lamellipodia and ruffles.

    GO - Cellular componenti

    1. apical plasma membrane Source: Ensembl
    2. external side of plasma membrane Source: MGI
    3. filopodium Source: UniProtKB
    4. filopodium membrane Source: UniProtKB-SubCell
    5. integral component of membrane Source: UniProtKB-KW
    6. lamellipodium Source: UniProtKB
    7. lamellipodium membrane Source: UniProtKB-SubCell
    8. microvillus membrane Source: UniProtKB-SubCell
    9. plasma membrane Source: MGI
    10. ruffle Source: UniProtKB
    11. ruffle membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Cell projection, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Mice die at birth of respiratory failure due to a low number of attenuated type I cells, narrow and irregular air spaces, and defective formation of alveolar saccules.1 Publication

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi34 – 341T → A: Eliminates platelet aggregation activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2222Sequence AnalysisAdd
    BLAST
    Chaini23 – 172150PodoplaninPRO_0000021352Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi37 – 371O-linked (GalNAc...)Sequence Analysis
    Glycosylationi51 – 511O-linked (GalNAc...)Sequence Analysis
    Glycosylationi52 – 521O-linked (GalNAc...)Sequence Analysis
    Glycosylationi53 – 531O-linked (GalNAc...)Sequence Analysis
    Glycosylationi56 – 561O-linked (GalNAc...)Sequence Analysis
    Glycosylationi60 – 601N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi63 – 631O-linked (GalNAc...)Sequence Analysis
    Glycosylationi71 – 711O-linked (GalNAc...)Sequence Analysis
    Glycosylationi77 – 771O-linked (GalNAc...)Sequence Analysis
    Glycosylationi85 – 851O-linked (GalNAc...)Sequence Analysis
    Glycosylationi86 – 861O-linked (GalNAc...)Sequence Analysis
    Glycosylationi87 – 871O-linked (GalNAc...)Sequence Analysis
    Glycosylationi89 – 891O-linked (GalNAc...)Sequence Analysis
    Glycosylationi90 – 901O-linked (GalNAc...)Sequence Analysis
    Glycosylationi100 – 1001O-linked (GalNAc...)Sequence Analysis
    Glycosylationi101 – 1011O-linked (GalNAc...)Sequence Analysis
    Glycosylationi102 – 1021O-linked (GalNAc...)Sequence Analysis
    Glycosylationi107 – 1071O-linked (GalNAc...)Sequence Analysis
    Glycosylationi115 – 1151O-linked (GalNAc...)Sequence Analysis

    Post-translational modificationi

    Extensively O-glycosylated. Contains sialic acid residues. O-glycosylation is necessary for platelet aggregation activity.2 Publications
    The N-terminus is blocked.By similarity

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PRIDEiQ62011.

    Expressioni

    Tissue specificityi

    Detected at high levels in lung and brain, at lower levels in kidney, stomach, liver, spleen and esophagus, and not detected in skin and small intestine. Expressed in epithelial cells of choroid plexus, ependyma, glomerulus and alveolus, in mesothelial cells and in endothelia of lymphatic vessels. Also expressed in stromal cells of peripheral lymphoid tissue and thymic epithelial cells. Detected in carcinoma cell lines and cultured fibroblasts. Expressed at higher levels in colon carcinomas than in normal colon tissue.3 Publications

    Inductioni

    Down-regulated by treatment with puromycin aminonucleoside.1 Publication

    Gene expression databases

    ArrayExpressiQ62011.
    BgeeiQ62011.
    CleanExiMM_PDPN.
    GenevestigatoriQ62011.

    Interactioni

    Protein-protein interaction databases

    IntActiQ62011. 1 interaction.
    STRINGi10090.ENSMUSP00000030317.

    Structurei

    Secondary structure

    1
    172
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi81 – 833

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3IETX-ray2.20Q/X76-84[»]
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini23 – 141119ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini163 – 17210Cytoplasmic

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei142 – 16221HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the podoplanin family.Curated

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG46902.
    GeneTreeiENSGT00390000000013.
    HOGENOMiHOG000231122.
    HOVERGENiHBG080131.
    InParanoidiA2A8J3.
    KOiK16778.
    OMAiNMATSHS.
    OrthoDBiEOG7DC25R.
    PhylomeDBiQ62011.
    TreeFamiTF337068.

    Family and domain databases

    InterProiIPR008783. Podoplanin.
    [Graphical view]
    PANTHERiPTHR16861. PTHR16861. 1 hit.
    PfamiPF05808. Podoplanin. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q62011-1 [UniParc]FASTAAdd to Basket

    « Hide

    MWTVPVLFWV LGSVWFWDSA QGGTIGVNED DIVTPGTGDG MVPPGIEDKI    50
    TTTGATGGLN ESTGKAPLVP TQRERGTKPP LEELSTSATS DHDHREHEST 100
    TTVKVVTSHS VDKKTSHPNR DNAGDETQTT DKKDGLPVVT LVGIIVGVLL 150
    AIGFVGGIFI VVMKKISGRF SP 172
    Length:172
    Mass (Da):18,233
    Last modified:November 1, 1997 - v2
    Checksum:iC035ED251918CE6F
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti29 – 313EDD → KNN in AAA37724. (PubMed:1402691)Curated
    Sequence conflicti38 – 392GD → EN in AAA37724. (PubMed:1402691)Curated
    Sequence conflicti170 – 1723FSP → SRPKELNRTGCSPNTSENKR ASNLPCSPSSSCGGR in AAA39866. (PubMed:2088477)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M73748 mRNA. Translation: AAA39866.1.
    M96645 mRNA. Translation: AAA37724.1.
    AJ250246 mRNA. Translation: CAB58997.1.
    AJ297944 mRNA. Translation: CAC16152.1.
    AY115493 Genomic DNA. Translation: AAM66761.1.
    AK158855 mRNA. Translation: BAE34695.1.
    AL611982 Genomic DNA. Translation: CAM21724.1.
    BC026551 mRNA. Translation: AAH26551.1.
    CCDSiCCDS38943.1.
    PIRiA54560.
    RefSeqiNP_001277751.1. NM_001290822.1.
    NP_034459.2. NM_010329.3.
    UniGeneiMm.2976.

    Genome annotation databases

    EnsembliENSMUST00000030317; ENSMUSP00000030317; ENSMUSG00000028583.
    ENSMUST00000181754; ENSMUSP00000137969; ENSMUSG00000096951.
    GeneIDi14726.
    KEGGimmu:14726.
    UCSCiuc008vqa.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M73748 mRNA. Translation: AAA39866.1 .
    M96645 mRNA. Translation: AAA37724.1 .
    AJ250246 mRNA. Translation: CAB58997.1 .
    AJ297944 mRNA. Translation: CAC16152.1 .
    AY115493 Genomic DNA. Translation: AAM66761.1 .
    AK158855 mRNA. Translation: BAE34695.1 .
    AL611982 Genomic DNA. Translation: CAM21724.1 .
    BC026551 mRNA. Translation: AAH26551.1 .
    CCDSi CCDS38943.1.
    PIRi A54560.
    RefSeqi NP_001277751.1. NM_001290822.1.
    NP_034459.2. NM_010329.3.
    UniGenei Mm.2976.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3IET X-ray 2.20 Q/X 76-84 [» ]
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q62011. 1 interaction.
    STRINGi 10090.ENSMUSP00000030317.

    Proteomic databases

    PRIDEi Q62011.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000030317 ; ENSMUSP00000030317 ; ENSMUSG00000028583 .
    ENSMUST00000181754 ; ENSMUSP00000137969 ; ENSMUSG00000096951 .
    GeneIDi 14726.
    KEGGi mmu:14726.
    UCSCi uc008vqa.1. mouse.

    Organism-specific databases

    CTDi 10630.
    MGIi MGI:103098. Pdpn.

    Phylogenomic databases

    eggNOGi NOG46902.
    GeneTreei ENSGT00390000000013.
    HOGENOMi HOG000231122.
    HOVERGENi HBG080131.
    InParanoidi A2A8J3.
    KOi K16778.
    OMAi NMATSHS.
    OrthoDBi EOG7DC25R.
    PhylomeDBi Q62011.
    TreeFami TF337068.

    Miscellaneous databases

    NextBioi 286753.
    PROi Q62011.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q62011.
    Bgeei Q62011.
    CleanExi MM_PDPN.
    Genevestigatori Q62011.

    Family and domain databases

    InterProi IPR008783. Podoplanin.
    [Graphical view ]
    PANTHERi PTHR16861. PTHR16861. 1 hit.
    Pfami PF05808. Podoplanin. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation of a gene sequence induced later by tumor-promoting 12-O-tetradecanoylphorbol-13-acetate in mouse osteoblastic cells (MC3T3-E1) and expressed constitutively in ras-transformed cells."
      Nose K., Saito H., Kuroki T.
      Cell Growth Differ. 1:511-518(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Characterization and cloning of a novel glycoprotein expressed by stromal cells in T-dependent areas of peripheral lymphoid tissues."
      Farr A.G., Berry M.L., Kim A., Nelson A.J., Welch M.P., Aruffo A.
      J. Exp. Med. 176:1477-1482(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: BALB/c.
    3. "Identification of PA2.26 antigen as a novel cell-surface mucin-type glycoprotein that induces plasma membrane extensions and increased motility in keratinocytes."
      Scholl F.G., Gamallo C., Vilaro S., Quintanilla M.
      J. Cell Sci. 112:4601-4613(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 24-30 AND 66-73, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, GLYCOSYLATION.
    4. "Cloning and expression of the mouse glomerular podoplanin homologue gp38P."
      Boucherot A., Schreiber R., Pavenstaedt H., Kunzelmann K.
      Nephrol. Dial. Transplant. 17:978-984(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, INDUCTION.
      Tissue: Kidney.
    5. "Cloning and characterization studies of mouse E11 gene and its spatial and temporal expression pattern during development."
      Lu Y., Zhang J., Harris M.A., Harris S.E., Bonewald L., Feng J.
      Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 129/Sv.
    6. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Visual cortex.
    7. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    8. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Colon.
    9. "T1alpha, a lung type I cell differentiation gene, is required for normal lung cell proliferation and alveolus formation at birth."
      Ramirez M.I., Millien G., Hinds A., Cao Y., Seldin D.C., Williams M.C.
      Dev. Biol. 256:61-72(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE.
    10. "Molecular identification of aggrus/T1alpha as a platelet aggregation-inducing factor expressed in colorectal tumors."
      Kato Y., Fujita N., Kunita A., Sato S., Kaneko M., Osawa M., Tsuruo T.
      J. Biol. Chem. 278:51599-51605(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY, MUTAGENESIS OF THR-34.
    11. "Functional sialylated O-glycan to platelet aggregation on Aggrus (T1alpha/Podoplanin) molecules expressed in Chinese hamster ovary cells."
      Kaneko M., Kato Y., Kunita A., Fujita N., Tsuruo T., Osawa M.
      J. Biol. Chem. 279:38838-38843(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, GLYCOSYLATION.
    12. Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF 76-84 IN COMPLEX WITH IMMUNOGLOBULIN.

    Entry informationi

    Entry nameiPDPN_MOUSE
    AccessioniPrimary (citable) accession number: Q62011
    Secondary accession number(s): A2A8J3, Q546R8, Q61612
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 107 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3