Ribonuclease 3 - Caenorhabditis briggsae

Preferred order of sections

Names and origin

Protein names

Recommended name:
Ribonuclease 3
EC=3.1.26.3

Alternative name(s):
Protein drosha
Ribonuclease III
Short name=RNase III

Gene names

Name:	drsh-1
ORF Names:	CBG03798

Organism

Caenorhabditis briggsae [Reference proteome]

Taxonomic identifier

6238 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	1061 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Executes the initial step of microRNA (miRNA) processing in the nucleus, that is the cleavage of pri-miRNA to release pre-miRNA. Involved in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA. Involved in fertility. Required for the function or synthesis of the let-7 miRNA By similarity. UniProtKB O01326 UniProtKB Q9NRR4
Catalytic activity	Endonucleolytic cleavage to 5'-phosphomonoester.
Cofactor	Magnesium or manganese By similarity.
Subcellular location	Nucleus By similarity UniProtKB Q9NRR4.
Sequence similarities	Contains 1 DRBM (double-stranded RNA-binding) domain. Contains 2 RNase III domains.

Ontologies

Keywords
Biological process	Differentiation Gonadal differentiation Ribosome biogenesis rRNA processing
Cellular component	Nucleus
Domain	Repeat
Ligand	Magnesium Manganese Metal-binding RNA-binding
Molecular function	Developmental protein Endonuclease Hydrolase Nuclease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW gonadal mesoderm development Inferred from electronic annotation. Source: UniProtKB-KW primary miRNA processing Inferred from sequence or structural similarity. Source: UniProtKB rRNA catabolic process Inferred from electronic annotation. Source: InterPro rRNA processing Inferred from electronic annotation. Source: UniProtKB-KW sexual reproduction Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	double-stranded RNA binding Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW ribonuclease III activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1061

1061

Ribonuclease 3

PRO_0000309457

Regions

Domain

586 – 759

174

RNase III 1

Domain

811 – 935

125

RNase III 2

Domain

962 – 1037

76

DRBM

Sites

Metal binding

851

1

Magnesium or manganese By similarity

Metal binding

921

1

Magnesium or manganese By similarity

Metal binding

924

1

Magnesium or manganese By similarity

Site

917

1

Important for activity By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q61XX9 [UniParc].

Last modified November 23, 2004. Version 1.
Checksum: 796401EAD0B5138E
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FASTA

1,061

122,481

        10         20         30         40         50         60 
MDFTEIHKRS RRKKFQQIHQ DRKDEMIQQL GRRFHNQPST SATYPSAVED IPLPSEVPNV 

        70         80         90        100        110        120 
FGAPPPLTNA DFHRNFLVDP DVVVSHSASL IRSNRHIVKA EDAEQYMMVS RERVGTTAER 

       130        140        150        160        170        180 
VLEDFNSRVI KPLKAKRRLQ IDVPYIDHPL HSMRSKTPER KENEEDSDSE IRSSDSSSDA 

       190        200        210        220        230        240 
EYGSDVEEEP DSCRRKKRTH KIQKADSSQT KVEEKERQNT LLRMGIERKR NHPNAIDPHI 

       250        260        270        280        290        300 
SYNEKGLGND SPECRCPFPI RNRGLKHGYY AGENQVLKCS KNDRANLHYY TLHVTPAPNE 

       310        320        330        340        350        360 
SQIQKTQMLI NGMEYHFEGF TMVTHAPLPD CMTRRPVFKY SIDYEFQLIE EFMPTECFDP 

       370        380        390        400        410        420 
EDCNSIFEYI FHDIFELLDF DLYPKHLPPG TASCPTIHIV PRFVAMENNT TFIWSSKTVL 

       430        440        450        460        470        480 
AFFLLHGKNN MFSPEDVEKN CAMSDDAFGR TIAKLKQSIV LNPMKKPSAL RADWFSRDLE 

       490        500        510        520        530        540 
NKEMFLIQNT IRSQNFASPF LPQIAALEKK MSRLKQEKKD SGNKNPHYEN LKAELIVLKD 

       550        560        570        580        590        600 
KHREARQLKL KLPVKDYIDT GLKPDVVAHV AMAIIASHHI RYNFSLSVFE KVIEYKFNDR 

       610        620        630        640        650        660 
RIVELALIHS SFRSYYGTTP DHVKNMISNC GYRKKYGAEE RREKKKGIIS LFNIMGGETS 

       670        680        690        700        710        720 
GGEPILHNER LEYLGDAVVE LIASHHLFFI LNHHFEGGLA TYRTALVQNR NLAKLAMNCR 

       730        740        750        760        770        780 
IDEMLQFAHG ADLINEAEWK HALANAFEAL MAGVFLDSGI APCDAIFSKA MYGKDPEMKK 

       790        800        810        820        830        840 
VWDHLNEHEL KIEDPLGDRD LSRITPALTD FHRLEQIIGI EFNNIRLLAK AFTRRNVPFN 

       850        860        870        880        890        900 
DLTKGHNQRL EWLGDSVLQL IISDYLYRNF PLHHEGHMSL LRTSLVSNQT QSVVCDDLGF 

       910        920        930        940        950        960 
QEFVIKAPHR KNDLKMKDKA DLVEAFIGAL YVDKGLEYCR SFIRTVFCPR LKHFINSEKW 

       970        980        990       1000       1010       1020 
NDAKSHLQQW CLAIRDSRNP NPAMPEYRLL GIQGPTNNRI FRVAVYFRGE RLSSAAASNM 

      1030       1040       1050       1060 
HTAELKAAEN ALAALEKASF SRMREKYMSG RQHRLHRIFF S

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References

[1]

"The genome sequence of Caenorhabditis briggsae: a platform for comparative genomics."
Stein L.D., Bao Z., Blasiar D., Blumenthal T., Brent M.R., Chen N., Chinwalla A., Clarke L., Clee C., Coghlan A., Coulson A., D'Eustachio P., Fitch D.H.A., Fulton L.A., Fulton R.E., Griffiths-Jones S., Harris T.W., Hillier L.W.

Waterston R.H.
PLoS Biol. 1:166-192(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AF16.

Cross-references

Sequence databases
EMBL GenBank DDBJ	HE600906 Genomic DNA. Translation: CAP24627.1.
RefSeq	XP_002639240.1. XM_002639194.1.
3D structure databases
HSSP	HSSP built from PDB template 1YYW based on UniProtKB O67082.
ProteinModelPortal	Q61XX9.
ModBase	Search...
Protein-protein interaction databases
STRING	6238.CBG03798.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	CBG03798; CBG03798; CBG03798.
GeneID	8581234.
KEGG	cbr:CBG03798.
Organism-specific databases
CTD	8581234.
WormBase	CBG03798; CBP21344; WBGene00026581; Cbr-drsh-1.
Phylogenomic databases
eggNOG	COG0571.
HOGENOM	HOG000154167.
KO	K03685.
OMA	FNIMGGE.
Family and domain databases
Gene3D	1.10.1520.10. 3 hits. 3.30.160.20. 1 hit.
InterPro	IPR001159. Ds-RNA-bd. IPR014720. dsRNA-bd-like_dom. IPR011907. RNase_III. IPR000999. RNase_III_dom. [Graphical view]
PANTHER	PTHR11207. PTHR11207. 1 hit.
Pfam	PF00035. dsrm. 1 hit. PF00636. Ribonuclease_3. 2 hits. [Graphical view]
SMART	SM00358. DSRM. 1 hit. SM00535. RIBOc. 2 hits. [Graphical view]
SUPFAM	SSF69065. RNase_III. 2 hits.
PROSITE	PS50137. DS_RBD. 1 hit. PS00517. RNASE_3_1. 2 hits. PS50142. RNASE_3_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNC_CAEBR

Accession

Primary (citable) accession number: Q61XX9
Secondary accession number(s): A8WWR6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 13, 2007
Last sequence update:	November 23, 2004
Last modified:	April 3, 2013
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Family and domain databases

Entry information

Relevant documents