Reviewed, UniProtKB/Swiss-Prot Q61XX9 (RNC_CAEBR)

Names and origin

Protein names

Recommended name:
    Ribonuclease 3
    EC=3.1.26.3
Alternative name(s):
    Ribonuclease III
      Short name=RNase III
    Protein drosher

Gene names

Name:	drsh-1
ORF Names:	CBG03798

Organism

Caenorhabditis briggsae [Complete proteome]

Taxonomic identifier

6238 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	1061 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Executes the initial step of microRNA (miRNA) processing in the nucleus, that is the cleavage of pri-miRNA to release pre-miRNA. Involved in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA. Involved in fertility. Required for the function or synthesis of the let-7 miRNA By similarity. UniProtKB O01326 UniProtKB Q9NRR4
Catalytic activity	Endonucleolytic cleavage to 5'-phosphomonoester.
Cofactor	Magnesium or manganese By similarity.
Subcellular location	Nucleus By similarity. UniProtKB Q9NRR4
Sequence similarities	Contains 1 DRBM (double-stranded RNA-binding) domain. Contains 2 RNase III domains.

Ontologies

Keywords
Biological process	Differentiation Gonadal differentiation Ribosome biogenesis rRNA processing
Cellular component	Nucleus
Domain	Repeat
Ligand	Magnesium Manganese Metal-binding RNA-binding
Molecular function	Developmental protein Endonuclease Hydrolase Nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW gonadal mesoderm development Inferred from electronic annotation. Source: UniProtKB-KW primary microRNA processing Inferred from sequence or structural similarity. Source: UniProtKB rRNA processing Inferred from electronic annotation. Source: UniProtKB-KW sexual reproduction Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	double-stranded RNA binding Inferred from electronic annotation. Source: InterPro magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW ribonuclease III activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1061	1061	Ribonuclease 3		PRO_0000309457
Regions
	Domain	586 – 759	174	RNase III 1
	Domain	811 – 935	125	RNase III 2
	Domain	962 – 1037	76	DRBM
Sites
	Metal binding	851	1	Magnesium or manganese By similarity
	Metal binding	921	1	Magnesium or manganese By similarity
	Metal binding	924	1	Magnesium or manganese By similarity
	Site	917	1	Important for activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q61XX9-1 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 796401EAD0B5138E	FASTA	1,061	122,481
	10 20 30 40 50 60 MDFTEIHKRS RRKKFQQIHQ DRKDEMIQQL GRRFHNQPST SATYPSAVED IPLPSEVPNV 70 80 90 100 110 120 FGAPPPLTNA DFHRNFLVDP DVVVSHSASL IRSNRHIVKA EDAEQYMMVS RERVGTTAER 130 140 150 160 170 180 VLEDFNSRVI KPLKAKRRLQ IDVPYIDHPL HSMRSKTPER KENEEDSDSE IRSSDSSSDA 190 200 210 220 230 240 EYGSDVEEEP DSCRRKKRTH KIQKADSSQT KVEEKERQNT LLRMGIERKR NHPNAIDPHI 250 260 270 280 290 300 SYNEKGLGND SPECRCPFPI RNRGLKHGYY AGENQVLKCS KNDRANLHYY TLHVTPAPNE 310 320 330 340 350 360 SQIQKTQMLI NGMEYHFEGF TMVTHAPLPD CMTRRPVFKY SIDYEFQLIE EFMPTECFDP 370 380 390 400 410 420 EDCNSIFEYI FHDIFELLDF DLYPKHLPPG TASCPTIHIV PRFVAMENNT TFIWSSKTVL 430 440 450 460 470 480 AFFLLHGKNN MFSPEDVEKN CAMSDDAFGR TIAKLKQSIV LNPMKKPSAL RADWFSRDLE 490 500 510 520 530 540 NKEMFLIQNT IRSQNFASPF LPQIAALEKK MSRLKQEKKD SGNKNPHYEN LKAELIVLKD 550 560 570 580 590 600 KHREARQLKL KLPVKDYIDT GLKPDVVAHV AMAIIASHHI RYNFSLSVFE KVIEYKFNDR 610 620 630 640 650 660 RIVELALIHS SFRSYYGTTP DHVKNMISNC GYRKKYGAEE RREKKKGIIS LFNIMGGETS 670 680 690 700 710 720 GGEPILHNER LEYLGDAVVE LIASHHLFFI LNHHFEGGLA TYRTALVQNR NLAKLAMNCR 730 740 750 760 770 780 IDEMLQFAHG ADLINEAEWK HALANAFEAL MAGVFLDSGI APCDAIFSKA MYGKDPEMKK 790 800 810 820 830 840 VWDHLNEHEL KIEDPLGDRD LSRITPALTD FHRLEQIIGI EFNNIRLLAK AFTRRNVPFN 850 860 870 880 890 900 DLTKGHNQRL EWLGDSVLQL IISDYLYRNF PLHHEGHMSL LRTSLVSNQT QSVVCDDLGF 910 920 930 940 950 960 QEFVIKAPHR KNDLKMKDKA DLVEAFIGAL YVDKGLEYCR SFIRTVFCPR LKHFINSEKW 970 980 990 1000 1010 1020 NDAKSHLQQW CLAIRDSRNP NPAMPEYRLL GIQGPTNNRI FRVAVYFRGE RLSSAAASNM 1030 1040 1050 1060 HTAELKAAEN ALAALEKASF SRMREKYMSG RQHRLHRIFF S

References

[1]

"The genome sequence of Caenorhabditis briggsae: a platform for comparative genomics." Stein L.D., Bao Z., Blasiar D., Blumenthal T., Brent M.R., Chen N., Chinwalla A., Clarke L., Clee C., Coghlan A., Coulson A., D'Eustachio P., Fitch D.H.A., Fulton L.A., Fulton R.E., Griffiths-Jones S., Harris T.W., Hillier L.W. , Kamath R., Kuwabara P.E., Mardis E.R., Marra M.A., Miner T.L., Minx P., Mullikin J.C., Plumb R.W., Rogers J., Schein J.E., Sohrmann M., Spieth J., Stajich J.E., Wei C., Willey D., Wilson R.K., Durbin R.M., Waterston R.H. PLoS Biol. 1:166-192(2003) [PubMed: 14624247] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AF16.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CAAC02000457 Genomic DNA. Translation: CAP24627.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GenomeReviews	Gene locus drsh-1 in contig CU538964_GR.
UCSC	F26E4.10a. c. elegans.
Organism-specific databases
WormBase	WBGene00026581. CBG03798.
Phylogenomic databases
OMA	HLRFHKS.
Enzyme and pathway databases
BRENDA	3.1.26.3. 261794.
Family and domain databases
InterPro	IPR001159. Ds-RNA_bd. IPR014720. dsRNA-bd-like. IPR000999. RNase_III. [Graphical view]
Gene3D	G3DSA:3.30.160.20. dsRNA-bd-like. 1 hit. G3DSA:1.10.1520.10. RNase_III. 2 hits.
Pfam	PF00035. dsrm. 1 hit. PF00636. Ribonuclease_3. 2 hits. [Graphical view]
SMART	SM00358. DSRM. 1 hit. SM00535. RIBOc. 2 hits. [Graphical view]
PROSITE	PS50137. DS_RBD. 1 hit. PS00517. RNASE_3_1. 2 hits. PS50142. RNASE_3_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNC_CAEBR

Accession

Primary (citable) accession number: Q61XX9
Secondary accession number(s): A8WWR6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 13, 2007
Last sequence update:	November 23, 2004
Last modified:	November 3, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project