Q61KR9 (CALR_CAEBR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Calreticulin

Gene names

Name:	crt-1
ORF Names:	CBG09253

Organism

Caenorhabditis briggsae [Reference proteome]

Taxonomic identifier

6238 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	396 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin may interact transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER By similarity.
Subcellular location	Endoplasmic reticulum lumen By similarity.
Domain	Can be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. The P-domain binds one molecule of calcium with high affinity, whereas the acidic C-domain binds multiple calcium ions with low affinity By similarity. The interaction with glycans occurs through a binding site in the globular lectin domain By similarity. The zinc binding sites are localized to the N-domain By similarity.
Sequence similarities	Belongs to the calreticulin family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum
Domain	Repeat Signal
Ligand	Calcium Lectin Metal-binding Zinc
Molecular function	Chaperone
PTM	Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein folding Inferred from electronic annotation. Source: InterPro
Cellular_component	endoplasmic reticulum lumen Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 15	15	Potential
	Chain	16 – 396	381	Calreticulin		PRO_0000246154
Regions
	Repeat	186 – 197	12	1-1
	Repeat	205 – 216	12	1-2
	Repeat	222 – 233	12	1-3
	Repeat	239 – 250	12	1-4
	Repeat	254 – 264	11	2-1
	Repeat	268 – 278	11	2-2
	Repeat	282 – 292	11	2-3
	Region	186 – 250	65	4 X approximate repeats
	Region	? – 192		N-domain
	Region	193 – 301	109	P-domain
	Region	254 – 292	39	3 X approximate repeats
	Region	302 – 396	95	C-domain
	Motif	393 – 396	4	Prevents secretion from ER By similarity
	Compositional bias	332 – 391	60	Asp/Glu/Lys-rich
Sites
	Binding site	105	1	Carbohydrate By similarity
	Binding site	107	1	Carbohydrate By similarity
	Binding site	124	1	Carbohydrate By similarity
	Binding site	131	1	Carbohydrate By similarity
	Binding site	312	1	Carbohydrate By similarity
Amino acid modifications
	Disulfide bond	101 ↔ 133		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q61KR9 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: CE7E53118485EFF7	FASTA	396	45,809
	10 20 30 40 50 60 MKSLCLLAIV AVVSAEVYFK EEFNDASWEK RWVQSKHKDD FGAFKLSAGK FFDVESRDQG 70 80 90 100 110 120 IQTSQDAKFY SRAAKFDKEF SNKGKTLVIQ YTVKHEQGID CGGGYVKVMR GDADLADFHG 130 140 150 160 170 180 ETPYNVMFGP DICGPTRRVH VILNYKGENK LIKKEITCKS DELTHLYTLI LNADNTYEVK 190 200 210 220 230 240 IDGESAQTGS LEEDWDLLPA KKIKDPDAKK PEDWDEREYI DDAEDVKPED WEKPEHIPDP 250 260 270 280 290 300 DAKKPEDWDD EMDGEWEPPM IDNPEYKGEW KPKQIKNPAY KGKWIHPEIE NPEYTPDDEL 310 320 330 340 350 360 YLYENWGAIG FDLWQVKSGT IFDNVLITDS VEEAEAHAAE TFDKLKTVEK EKKEKADEEA 370 380 390 RKVEEEARKK AEEEKEAKKD DDEEEEKEEE EGHDEL

References

[1]

"The genome sequence of Caenorhabditis briggsae: a platform for comparative genomics." Stein L.D., Bao Z., Blasiar D., Blumenthal T., Brent M.R., Chen N., Chinwalla A., Clarke L., Clee C., Coghlan A., Coulson A., D'Eustachio P., Fitch D.H.A., Fulton L.A., Fulton R.E., Griffiths-Jones S., Harris T.W., Hillier L.W. , Kamath R., Kuwabara P.E., Mardis E.R., Marra M.A., Miner T.L., Minx P., Mullikin J.C., Plumb R.W., Rogers J., Schein J.E., Sohrmann M., Spieth J., Stajich J.E., Wei C., Willey D., Wilson R.K., Durbin R.M., Waterston R.H. PLoS Biol. 1:166-192(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AF16.

Cross-references

Sequence databases
EMBL GenBank DDBJ	HE600954 Genomic DNA. Translation: CAP29301.1.
RefSeq	XP_002636809.1. XM_002636763.1.
3D structure databases
ProteinModelPortal	Q61KR9.
SMR	Q61KR9. Positions 200-297.
ModBase	Search...
Protein-protein interaction databases
STRING	6238.CBG09253.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	CBG09253; CBG09253; CBG09253.
GeneID	8578804.
KEGG	cbr:CBG09253.
Organism-specific databases
CTD	8578804.
WormBase	CBG09253; CBP02265; WBGene00030869; Cbr-crt-1.
Phylogenomic databases
eggNOG	NOG305105.
HOGENOM	HOG000192435.
KO	K08057.
OMA	DGWTDRW.
Family and domain databases
Gene3D	2.60.120.200. 2 hits.
InterPro	IPR001580. Calret/calnex. IPR018124. Calret/calnex_CS. IPR009169. Calreticulin. IPR008985. ConA-like_lec_gl_sf. IPR013320. ConA-like_subgrp. [Graphical view]
PANTHER	PTHR11073. PTHR11073. 1 hit.
Pfam	PF00262. Calreticulin. 1 hit. [Graphical view]
PIRSF	PIRSF002356. Calreticulin. 1 hit.
PRINTS	PR00626. CALRETICULIN.
SUPFAM	SSF49899. ConA_like_lec_gl. 1 hit.
PROSITE	PS00803. CALRETICULIN_1. 1 hit. PS00804. CALRETICULIN_2. 1 hit. PS00805. CALRETICULIN_REPEAT. 3 hits. PS00014. ER_TARGET. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CALR_CAEBR

Accession

Primary (citable) accession number: Q61KR9
Secondary accession number(s): A8X9I9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	November 23, 2004
Last modified:	April 3, 2013
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project