Reviewed, UniProtKB/Swiss-Prot Q61E36 (MCES_CAEBR)

Names and origin

Protein names

Recommended name:
    mRNA cap guanine-N7 methyltransferase
    EC=2.1.1.56
Alternative name(s):
    mRNA (guanine-N(7)-)-methyltransferase

Gene names

Name:	tag-72
ORF Names:	CBG12234

Organism

Caenorhabditis briggsae [Complete proteome]

Taxonomic identifier

6238 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	378 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	mRNA-capping methyltransferase that methylates the N7 position of the added guanosine to the 5'-cap structure of mRNAs. Binds RNA containing 5'-terminal GpppC By similarity.
Catalytic activity	S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the methyltransferase superfamily. mRNA cap methyltransferase family.
Sequence caution	The sequence CAP31197.2 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	mRNA capping mRNA processing
Cellular component	Nucleus
Ligand	RNA-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	mRNA capping Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	nucleus Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	RNA binding Inferred from sequence or structural similarity. Source: UniProtKB mRNA (guanine-N7-)-methyltransferase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 378	378	mRNA cap guanine-N7 methyltransferase		PRO_0000248328
Regions
	Region	33 – 34	2	mRNA cap binding By similarity
Sites
	Binding site	37	1	S-adenosyl-L-methionine By similarity
	Binding site	62	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	65	1	mRNA cap By similarity
	Binding site	71	1	mRNA cap By similarity
	Binding site	84	1	S-adenosyl-L-methionine By similarity
	Binding site	96	1	mRNA cap By similarity
	Binding site	116	1	S-adenosyl-L-methionine By similarity
	Binding site	138	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	142	1	mRNA cap By similarity
	Binding site	229	1	mRNA cap By similarity
	Binding site	323	1	mRNA cap By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q61E36-1 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 142763C0CADEA65F	FASTA	378	42,956
	10 20 30 40 50 60 MSAAEVADHY NNVRQAGIQD RKESRIFFMR NMNNWIKSQL INDAMKLVNE NGVKSPVVLD 70 80 90 100 110 120 IACGKGGDLR KWDITGAKHV VMADVADVSI QQAEERYKTM HKYPHDIFGA QFIVADCTKE 130 140 150 160 170 180 NLDDKIEIKE PFDLVSCQFA MHYSFVDEDS ARTFLKNAVG KLKLGGVFIG TLPDADRIVW 190 200 210 220 230 240 AVRNGTEGKF ANDVCKITYE KVDELSEGNV PLFGAKFHFS LDEQVNCPEF LAYFSLVKHL 250 260 270 280 290 300 LEEHDMELLF VHNFAEAITN WLVPGRRLLE SMKGLETFPN RNLSGKTDEE YLEAKAKIDS 310 320 330 340 350 360 LGDNVPKFVG TLSKSEWEAI CMYLVFGFRK KKTTEKNLES EAPEIKKVTP VPLNEDTDKT 370 AEKNEERIEE KEENPSHC

References

[1]

"The genome sequence of Caenorhabditis briggsae: a platform for comparative genomics." Stein L.D., Bao Z., Blasiar D., Blumenthal T., Brent M.R., Chen N., Chinwalla A., Clarke L., Clee C., Coghlan A., Coulson A., D'Eustachio P., Fitch D.H.A., Fulton L.A., Fulton R.E., Griffiths-Jones S., Harris T.W., Hillier L.W. , Kamath R., Kuwabara P.E., Mardis E.R., Marra M.A., Miner T.L., Minx P., Mullikin J.C., Plumb R.W., Rogers J., Schein J.E., Sohrmann M., Spieth J., Stajich J.E., Wei C., Willey D., Wilson R.K., Durbin R.M., Waterston R.H. PLoS Biol. 1:166-192(2003) [PubMed: 14624247] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AF16.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CAAC02000508 Genomic DNA. Translation: CAP31197.2. Sequence problems.
RefSeq	XP_002639878.1.
3D structure databases
HSSP	HSSP built from PDB template 1RI5 based on UniProtKB Q8SR66.
SMR	Q61E36. Positions 25-332.
ModBase	Search...
Genome annotation databases
GeneID	8581872.
Organism-specific databases
WormBase	WBGene00033215. Cbr-tag-72.
Phylogenomic databases
HOGENOM	HBG377892.
OMA	KWRKGRI.
PhylomeDB	Q61E36.
Enzyme and pathway databases
BRENDA	2.1.1.56. 261794.
Family and domain databases
InterPro	IPR016899. mRNA_G-N7_MeTrfase. IPR004971. Pox_MCEL. [Graphical view]
Pfam	PF03291. Pox_MCEL. 1 hit. [Graphical view]
PIRSF	PIRSF028762. ABD1. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MCES_CAEBR

Accession

Primary (citable) accession number: Q61E36
Secondary accession number(s): A8XEX5

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	November 23, 2004
Last modified:	February 9, 2010
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project