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Q61990 (PCBP2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Poly(rC)-binding protein 2
Alternative name(s):
Alpha-CP2
CTBP
Short name=CBP
Putative heterogeneous nuclear ribonucleoprotein X
Short name=hnRNP X
Gene names
Name:Pcbp2
Synonyms:Cbp, Hnrnpx, Hnrpx
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length362 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Single-stranded nucleic acid binding protein that binds preferentially to oligo dC. Major cellular poly(rC)-binding protein. Binds also poly(rU). Negatively regulates cellular antiviral responses mediated by MAVS signaling. It acts as an adapter between MAVS and the E3 ubiquitin ligase ITCH, therefore triggering MAVS ubiquitinationa and degradation By similarity.

Subunit structure

Identified in a mRNP complex, at least composed of DHX9, DDX3X, ELAVL1, HNRNPU, IGF2BP1, ILF3, PABPC1, PCBP2, PTBP2, STAU1, STAU2, SYNCRIP and YBX1. Interacts with IFIH1 and RNF135 By similarity. Interacts with MAVS (via C-terminus) and ITCH (via WW domains) By similarity.

Subcellular location

Nucleus By similarity. Cytoplasm By similarity.

Domain

The KH domains mediates poly(C) binding By similarity.

Sequence similarities

Contains 3 KH domains.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q61990-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q61990-2)

The sequence of this isoform differs from the canonical sequence as follows:
     194-224: Missing.
Isoform 3 (identifier: Q61990-3)

The sequence of this isoform differs from the canonical sequence as follows:
     263-275: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 362362Poly(rC)-binding protein 2
PRO_0000050091

Regions

Domain13 – 7563KH 1
Domain97 – 16266KH 2
Domain284 – 34865KH 3

Amino acid modifications

Modified residue1691Phosphoserine By similarity
Modified residue1851Phosphoserine By similarity
Modified residue2681Phosphoserine By similarity
Modified residue3611Phosphoserine By similarity

Natural variations

Alternative sequence194 – 22431Missing in isoform 2.
VSP_002820
Alternative sequence263 – 27513Missing in isoform 3.
VSP_002821

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 70C8AF710E3BF3C0

FASTA36238,222
        10         20         30         40         50         60 
MDTGVIEGGL NVTLTIRLLM HGKEVGSIIG KKGESVKKMR EESGARINIS EGNCPERIIT 

        70         80         90        100        110        120 
LAGPTNAIFK AFAMIIDKLE EDISSSMTNS TAASRPPVTL RLVVPASQCG SLIGKGGCKI 

       130        140        150        160        170        180 
KEIRESTGAQ VQVAGDMLPN STERAITIAG IPQSIIECVK QICVVMLESP PKGVTIPYRP 

       190        200        210        220        230        240 
KPSSSPVIFA GGQDRYSTGS DSASFPHTTP SMCLNPDLEG PPLEAYTIQG QYAIPQPDLT 

       250        260        270        280        290        300 
KLHQLAMQQS HFPMTHGNTG FSGIESSSPE VKGYWAGLDA SAQTTSHELT IPNDLIGCII 

       310        320        330        340        350        360 
GRQGAKINEI RQMSGAQIKI ANPVEGSTDR QVTITGSAAS ISLAQYLINV RLSSETGGMG 


SS

« Hide

Isoform 2 [UniParc].

Checksum: E831D4BF718C26E3
Show »

FASTA33134,917
Isoform 3 [UniParc].

Checksum: E0699D574B0B7DDA
Show »

FASTA34936,801

References

[1]"Isolation of a murine gene encoding a nucleic acid-binding protein with homology to hnRNP K."
Hahm K.B., Kim G., Turch C., Smale S.T.
Nucleic Acids Res. 21:3894-3894(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
[2]"Murine protein which binds preferentially to oligo-C-rich single-stranded nucleic acids."
Goller M., Funke B., Gehe-Becker C., Kroeger B., Lottspeich F., Horak I.
Nucleic Acids Res. 22:1885-1889(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE (ISOFORM 2).
Strain: C57BL/6 X 129/Ola.
Tissue: Liver.
[3]Horak I.
Submitted (MAY-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE (ISOFORM 3).
Strain: C57BL/6 X CBA.
Tissue: Liver.
[4]"Identification of two novel mammalian genes establishes a subfamily of KH-domain RNA-binding proteins."
Makeyev A.V., Liebhaber S.A.
Genomics 67:301-316(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE (ISOFORM 1).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		L19661 mRNA. Translation: AAA03705.1.
X75947 mRNA. Translation: CAA53546.1.
X97982 mRNA. Translation: CAA66619.1.
AF236845 expand/collapse EMBL AC list , AF236842, AF236843, AF236844 Genomic DNA. Translation: AAK14059.1.
IPIIPI00127707.
IPI00221796.
IPI00221799.
PIRS78515.
RefSeqNP_001096635.1. NM_001103165.1.
NP_001096636.1. NM_001103166.1.
NP_035172.2. NM_011042.2.
UniGeneMm.236513.

3D structure databases

ProteinModelPortalQ61990.
SMRQ61990. Positions 11-169, 283-355.
ModBaseSearch...

Protein-protein interaction databases

IntActQ61990. 4 interactions.

PTM databases

PhosphoSiteQ61990.

2D gel databases

REPRODUCTION-2DPAGEQ61990.

Proteomic databases

PaxDbQ61990.
PRIDEQ61990.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000077037; ENSMUSP00000076294; ENSMUSG00000056851.
ENSMUST00000078404; ENSMUSP00000077509; ENSMUSG00000056851.
ENSMUST00000108838; ENSMUSP00000104466; ENSMUSG00000056851.
GeneID18521.
KEGGmmu:18521.
UCSCuc007xvy.2. mouse.
uc007xvz.2. mouse.
uc009vat.1. mouse.

Organism-specific databases

CTD5094.
MGIMGI:108202. Pcbp2.

Phylogenomic databases

eggNOGNOG315872.
GeneTreeENSGT00550000074311.
HOGENOMHOG000182823.
HOVERGENHBG053520.
InParanoidQ61990.
KOK13162.
OMACVVMLEL.
OrthoDBEOG46WZ8K.

Gene expression databases

ArrayExpressQ61990.
BgeeQ61990.
CleanExMM_PCBP2.
GenevestigatorQ61990.
GermOnlineENSMUSG00000056851. Mus musculus.

Family and domain databases

InterProIPR004087. KH_dom.
IPR004088. KH_dom_type_1.
[Graphical view]
PfamPF00013. KH_1. 3 hits.
[Graphical view]
SMARTSM00322. KH. 3 hits.
[Graphical view]
PROSITEPS50084. KH_TYPE_1. 3 hits.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSPCBP2. mouse.
NextBio294276.
SOURCESearch...

Entry information

Entry namePCBP2_MOUSE
AccessionPrimary (citable) accession number: Q61990
Secondary accession number(s): Q61383, Q62042
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: April 3, 2013
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

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