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Protein

Poly(rC)-binding protein 2

Gene

Pcbp2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Single-stranded nucleic acid binding protein that binds preferentially to oligo dC. Major cellular poly(rC)-binding protein. Binds also poly(rU). Negatively regulates cellular antiviral responses mediated by MAVS signaling. It acts as an adapter between MAVS and the E3 ubiquitin ligase ITCH, therefore triggering MAVS ubiquitinationa and degradation (By similarity).By similarity

GO - Molecular functioni

  • C-rich single-stranded DNA binding Source: MGI
  • DNA binding Source: MGI
  • enzyme binding Source: MGI
  • poly(A) RNA binding Source: MGI
  • RNA binding Source: MGI
  • ubiquitin protein ligase binding Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Biological processi

Antiviral defense, Immunity, Innate immunity

Keywords - Ligandi

DNA-binding, RNA-binding

Enzyme and pathway databases

ReactomeiR-MMU-72163. mRNA Splicing - Major Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Poly(rC)-binding protein 2
Alternative name(s):
Alpha-CP2
CTBP
Short name:
CBP
Putative heterogeneous nuclear ribonucleoprotein X
Short name:
hnRNP X
Gene namesi
Name:Pcbp2
Synonyms:Cbp, Hnrnpx, Hnrpx
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:108202. Pcbp2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 362362Poly(rC)-binding protein 2PRO_0000050091Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei169 – 1691PhosphoserineBy similarity
Modified residuei185 – 1851PhosphoserineBy similarity
Modified residuei268 – 2681PhosphoserineBy similarity
Modified residuei361 – 3611PhosphoserineBy similarity
Modified residuei362 – 3621PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ61990.
MaxQBiQ61990.
PaxDbiQ61990.
PRIDEiQ61990.

2D gel databases

REPRODUCTION-2DPAGEQ61990.

PTM databases

iPTMnetiQ61990.
PhosphoSiteiQ61990.

Expressioni

Gene expression databases

BgeeiQ61990.
CleanExiMM_PCBP2.
GenevisibleiQ61990. MM.

Interactioni

Subunit structurei

Identified in a mRNP complex, at least composed of DHX9, DDX3X, ELAVL1, HNRNPU, IGF2BP1, ILF3, PABPC1, PCBP2, PTBP2, STAU1, STAU2, SYNCRIP and YBX1. Interacts with IFIH1 and RNF135 (By similarity). Interacts with MAVS (via C-terminus) and ITCH (via WW domains) (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi202043. 12 interactions.
IntActiQ61990. 18 interactions.
MINTiMINT-1520086.
STRINGi10090.ENSMUSP00000076294.

Structurei

3D structure databases

ProteinModelPortaliQ61990.
SMRiQ61990. Positions 11-169, 283-355.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini13 – 7563KH 1PROSITE-ProRule annotationAdd
BLAST
Domaini97 – 16266KH 2PROSITE-ProRule annotationAdd
BLAST
Domaini284 – 34865KH 3PROSITE-ProRule annotationAdd
BLAST

Domaini

The KH domains mediates poly(C) binding.By similarity

Sequence similaritiesi

Contains 3 KH domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG2190. Eukaryota.
ENOG410XNN8. LUCA.
GeneTreeiENSGT00760000119144.
HOGENOMiHOG000182823.
HOVERGENiHBG053520.
InParanoidiQ61990.
KOiK13162.
OMAiCWHELEQ.
OrthoDBiEOG7P02J4.
PhylomeDBiQ61990.
TreeFamiTF318292.

Family and domain databases

Gene3Di3.30.1370.10. 3 hits.
InterProiIPR004087. KH_dom.
IPR004088. KH_dom_type_1.
[Graphical view]
PfamiPF00013. KH_1. 3 hits.
[Graphical view]
SMARTiSM00322. KH. 3 hits.
[Graphical view]
SUPFAMiSSF54791. SSF54791. 3 hits.
PROSITEiPS50084. KH_TYPE_1. 3 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q61990-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDTGVIEGGL NVTLTIRLLM HGKEVGSIIG KKGESVKKMR EESGARINIS
60 70 80 90 100
EGNCPERIIT LAGPTNAIFK AFAMIIDKLE EDISSSMTNS TAASRPPVTL
110 120 130 140 150
RLVVPASQCG SLIGKGGCKI KEIRESTGAQ VQVAGDMLPN STERAITIAG
160 170 180 190 200
IPQSIIECVK QICVVMLESP PKGVTIPYRP KPSSSPVIFA GGQDRYSTGS
210 220 230 240 250
DSASFPHTTP SMCLNPDLEG PPLEAYTIQG QYAIPQPDLT KLHQLAMQQS
260 270 280 290 300
HFPMTHGNTG FSGIESSSPE VKGYWAGLDA SAQTTSHELT IPNDLIGCII
310 320 330 340 350
GRQGAKINEI RQMSGAQIKI ANPVEGSTDR QVTITGSAAS ISLAQYLINV
360
RLSSETGGMG SS
Length:362
Mass (Da):38,222
Last modified:November 1, 1996 - v1
Checksum:i70C8AF710E3BF3C0
GO
Isoform 2 (identifier: Q61990-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     194-224: Missing.

Show »
Length:331
Mass (Da):34,917
Checksum:iE831D4BF718C26E3
GO
Isoform 3 (identifier: Q61990-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     263-275: Missing.

Show »
Length:349
Mass (Da):36,801
Checksum:iE0699D574B0B7DDA
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei194 – 22431Missing in isoform 2. 1 PublicationVSP_002820Add
BLAST
Alternative sequencei263 – 27513Missing in isoform 3. CuratedVSP_002821Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L19661 mRNA. Translation: AAA03705.1.
X75947 mRNA. Translation: CAA53546.1.
X97982 mRNA. Translation: CAA66619.1.
AF236845
, AF236842, AF236843, AF236844 Genomic DNA. Translation: AAK14059.1.
CCDSiCCDS27884.1. [Q61990-3]
CCDS49741.1. [Q61990-1]
CCDS49742.1. [Q61990-2]
PIRiS78515.
RefSeqiNP_001096635.1. NM_001103165.1. [Q61990-1]
NP_001096636.1. NM_001103166.1. [Q61990-2]
NP_035172.2. NM_011042.2. [Q61990-3]
UniGeneiMm.236513.

Genome annotation databases

EnsembliENSMUST00000077037; ENSMUSP00000076294; ENSMUSG00000056851. [Q61990-1]
ENSMUST00000078404; ENSMUSP00000077509; ENSMUSG00000056851. [Q61990-3]
ENSMUST00000108838; ENSMUSP00000104466; ENSMUSG00000056851. [Q61990-2]
GeneIDi18521.
KEGGimmu:18521.
UCSCiuc007xvy.2. mouse. [Q61990-1]
uc007xvz.2. mouse. [Q61990-2]
uc009vat.1. mouse. [Q61990-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L19661 mRNA. Translation: AAA03705.1.
X75947 mRNA. Translation: CAA53546.1.
X97982 mRNA. Translation: CAA66619.1.
AF236845
, AF236842, AF236843, AF236844 Genomic DNA. Translation: AAK14059.1.
CCDSiCCDS27884.1. [Q61990-3]
CCDS49741.1. [Q61990-1]
CCDS49742.1. [Q61990-2]
PIRiS78515.
RefSeqiNP_001096635.1. NM_001103165.1. [Q61990-1]
NP_001096636.1. NM_001103166.1. [Q61990-2]
NP_035172.2. NM_011042.2. [Q61990-3]
UniGeneiMm.236513.

3D structure databases

ProteinModelPortaliQ61990.
SMRiQ61990. Positions 11-169, 283-355.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202043. 12 interactions.
IntActiQ61990. 18 interactions.
MINTiMINT-1520086.
STRINGi10090.ENSMUSP00000076294.

PTM databases

iPTMnetiQ61990.
PhosphoSiteiQ61990.

2D gel databases

REPRODUCTION-2DPAGEQ61990.

Proteomic databases

EPDiQ61990.
MaxQBiQ61990.
PaxDbiQ61990.
PRIDEiQ61990.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000077037; ENSMUSP00000076294; ENSMUSG00000056851. [Q61990-1]
ENSMUST00000078404; ENSMUSP00000077509; ENSMUSG00000056851. [Q61990-3]
ENSMUST00000108838; ENSMUSP00000104466; ENSMUSG00000056851. [Q61990-2]
GeneIDi18521.
KEGGimmu:18521.
UCSCiuc007xvy.2. mouse. [Q61990-1]
uc007xvz.2. mouse. [Q61990-2]
uc009vat.1. mouse. [Q61990-3]

Organism-specific databases

CTDi5094.
MGIiMGI:108202. Pcbp2.

Phylogenomic databases

eggNOGiKOG2190. Eukaryota.
ENOG410XNN8. LUCA.
GeneTreeiENSGT00760000119144.
HOGENOMiHOG000182823.
HOVERGENiHBG053520.
InParanoidiQ61990.
KOiK13162.
OMAiCWHELEQ.
OrthoDBiEOG7P02J4.
PhylomeDBiQ61990.
TreeFamiTF318292.

Enzyme and pathway databases

ReactomeiR-MMU-72163. mRNA Splicing - Major Pathway.

Miscellaneous databases

ChiTaRSiPcbp2. mouse.
PROiQ61990.
SOURCEiSearch...

Gene expression databases

BgeeiQ61990.
CleanExiMM_PCBP2.
GenevisibleiQ61990. MM.

Family and domain databases

Gene3Di3.30.1370.10. 3 hits.
InterProiIPR004087. KH_dom.
IPR004088. KH_dom_type_1.
[Graphical view]
PfamiPF00013. KH_1. 3 hits.
[Graphical view]
SMARTiSM00322. KH. 3 hits.
[Graphical view]
SUPFAMiSSF54791. SSF54791. 3 hits.
PROSITEiPS50084. KH_TYPE_1. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation of a murine gene encoding a nucleic acid-binding protein with homology to hnRNP K."
    Hahm K.B., Kim G., Turch C., Smale S.T.
    Nucleic Acids Res. 21:3894-3894(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
  2. "Murine protein which binds preferentially to oligo-C-rich single-stranded nucleic acids."
    Goller M., Funke B., Gehe-Becker C., Kroeger B., Lottspeich F., Horak I.
    Nucleic Acids Res. 22:1885-1889(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE (ISOFORM 2).
    Strain: C57BL/6 X 129/Ola.
    Tissue: Liver.
  3. Horak I.
    Submitted (MAY-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE (ISOFORM 3).
    Strain: C57BL/6 X CBA.
    Tissue: Liver.
  4. "Identification of two novel mammalian genes establishes a subfamily of KH-domain RNA-binding proteins."
    Makeyev A.V., Liebhaber S.A.
    Genomics 67:301-316(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE (ISOFORM 1).
  5. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
    Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
    Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiPCBP2_MOUSE
AccessioniPrimary (citable) accession number: Q61990
Secondary accession number(s): Q61383, Q62042
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: June 8, 2016
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.