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Protein

Poly(rC)-binding protein 2

Gene

Pcbp2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Single-stranded nucleic acid binding protein that binds preferentially to oligo dC. Major cellular poly(rC)-binding protein. Binds also poly(rU). Negatively regulates cellular antiviral responses mediated by MAVS signaling. It acts as an adapter between MAVS and the E3 ubiquitin ligase ITCH, therefore triggering MAVS ubiquitinationa and degradation (By similarity).By similarity

GO - Molecular functioni

  • C-rich single-stranded DNA binding Source: MGI
  • DNA binding Source: MGI
  • enzyme binding Source: MGI
  • poly(A) RNA binding Source: MGI
  • RNA binding Source: MGI
  • ubiquitin protein ligase binding Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Biological processi

Antiviral defense, Immunity, Innate immunity

Keywords - Ligandi

DNA-binding, RNA-binding

Enzyme and pathway databases

ReactomeiR-MMU-72163. mRNA Splicing - Major Pathway.
R-MMU-72203. Processing of Capped Intron-Containing Pre-mRNA.

Names & Taxonomyi

Protein namesi
Recommended name:
Poly(rC)-binding protein 2
Alternative name(s):
Alpha-CP2
CTBP
Short name:
CBP
Putative heterogeneous nuclear ribonucleoprotein X
Short name:
hnRNP X
Gene namesi
Name:Pcbp2
Synonyms:Cbp, Hnrnpx, Hnrpx
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:108202. Pcbp2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000500911 – 362Poly(rC)-binding protein 2Add BLAST362

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei169PhosphoserineBy similarity1
Modified residuei185PhosphoserineBy similarity1
Modified residuei268PhosphoserineBy similarity1
Modified residuei361PhosphoserineBy similarity1
Modified residuei362PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ61990.
PaxDbiQ61990.
PeptideAtlasiQ61990.
PRIDEiQ61990.

2D gel databases

REPRODUCTION-2DPAGEQ61990.

PTM databases

iPTMnetiQ61990.
PhosphoSitePlusiQ61990.

Expressioni

Gene expression databases

BgeeiENSMUSG00000056851.
CleanExiMM_PCBP2.
GenevisibleiQ61990. MM.

Interactioni

Subunit structurei

Identified in a mRNP complex, at least composed of DHX9, DDX3X, ELAVL1, HNRNPU, IGF2BP1, ILF3, PABPC1, PCBP2, PTBP2, STAU1, STAU2, SYNCRIP and YBX1. Interacts with IFIH1 and RNF135 (By similarity). Interacts with MAVS (via C-terminus) and ITCH (via WW domains) (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi202043. 12 interactors.
IntActiQ61990. 18 interactors.
MINTiMINT-1520086.
STRINGi10090.ENSMUSP00000076294.

Structurei

3D structure databases

ProteinModelPortaliQ61990.
SMRiQ61990.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini13 – 75KH 1PROSITE-ProRule annotationAdd BLAST63
Domaini97 – 162KH 2PROSITE-ProRule annotationAdd BLAST66
Domaini284 – 348KH 3PROSITE-ProRule annotationAdd BLAST65

Domaini

The KH domains mediates poly(C) binding.By similarity

Sequence similaritiesi

Contains 3 KH domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG2190. Eukaryota.
ENOG410XNN8. LUCA.
GeneTreeiENSGT00760000119144.
HOGENOMiHOG000182823.
HOVERGENiHBG053520.
InParanoidiQ61990.
KOiK13162.
OMAiCWHELEQ.
OrthoDBiEOG091G0DHI.
PhylomeDBiQ61990.
TreeFamiTF318292.

Family and domain databases

Gene3Di3.30.1370.10. 3 hits.
InterProiIPR004087. KH_dom.
IPR004088. KH_dom_type_1.
[Graphical view]
PfamiPF00013. KH_1. 3 hits.
[Graphical view]
SMARTiSM00322. KH. 3 hits.
[Graphical view]
SUPFAMiSSF54791. SSF54791. 3 hits.
PROSITEiPS50084. KH_TYPE_1. 3 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q61990-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDTGVIEGGL NVTLTIRLLM HGKEVGSIIG KKGESVKKMR EESGARINIS
60 70 80 90 100
EGNCPERIIT LAGPTNAIFK AFAMIIDKLE EDISSSMTNS TAASRPPVTL
110 120 130 140 150
RLVVPASQCG SLIGKGGCKI KEIRESTGAQ VQVAGDMLPN STERAITIAG
160 170 180 190 200
IPQSIIECVK QICVVMLESP PKGVTIPYRP KPSSSPVIFA GGQDRYSTGS
210 220 230 240 250
DSASFPHTTP SMCLNPDLEG PPLEAYTIQG QYAIPQPDLT KLHQLAMQQS
260 270 280 290 300
HFPMTHGNTG FSGIESSSPE VKGYWAGLDA SAQTTSHELT IPNDLIGCII
310 320 330 340 350
GRQGAKINEI RQMSGAQIKI ANPVEGSTDR QVTITGSAAS ISLAQYLINV
360
RLSSETGGMG SS
Length:362
Mass (Da):38,222
Last modified:November 1, 1996 - v1
Checksum:i70C8AF710E3BF3C0
GO
Isoform 2 (identifier: Q61990-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     194-224: Missing.

Show »
Length:331
Mass (Da):34,917
Checksum:iE831D4BF718C26E3
GO
Isoform 3 (identifier: Q61990-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     263-275: Missing.

Show »
Length:349
Mass (Da):36,801
Checksum:iE0699D574B0B7DDA
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_002820194 – 224Missing in isoform 2. 1 PublicationAdd BLAST31
Alternative sequenceiVSP_002821263 – 275Missing in isoform 3. CuratedAdd BLAST13

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L19661 mRNA. Translation: AAA03705.1.
X75947 mRNA. Translation: CAA53546.1.
X97982 mRNA. Translation: CAA66619.1.
AF236845
, AF236842, AF236843, AF236844 Genomic DNA. Translation: AAK14059.1.
CCDSiCCDS27884.1. [Q61990-3]
CCDS49741.1. [Q61990-1]
CCDS49742.1. [Q61990-2]
PIRiS78515.
RefSeqiNP_001096635.1. NM_001103165.1. [Q61990-1]
NP_001096636.1. NM_001103166.1. [Q61990-2]
NP_035172.2. NM_011042.2. [Q61990-3]
UniGeneiMm.236513.

Genome annotation databases

EnsembliENSMUST00000077037; ENSMUSP00000076294; ENSMUSG00000056851. [Q61990-1]
ENSMUST00000078404; ENSMUSP00000077509; ENSMUSG00000056851. [Q61990-3]
ENSMUST00000108838; ENSMUSP00000104466; ENSMUSG00000056851. [Q61990-2]
GeneIDi18521.
KEGGimmu:18521.
UCSCiuc007xvy.2. mouse. [Q61990-1]
uc007xvz.2. mouse. [Q61990-2]
uc009vat.1. mouse. [Q61990-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L19661 mRNA. Translation: AAA03705.1.
X75947 mRNA. Translation: CAA53546.1.
X97982 mRNA. Translation: CAA66619.1.
AF236845
, AF236842, AF236843, AF236844 Genomic DNA. Translation: AAK14059.1.
CCDSiCCDS27884.1. [Q61990-3]
CCDS49741.1. [Q61990-1]
CCDS49742.1. [Q61990-2]
PIRiS78515.
RefSeqiNP_001096635.1. NM_001103165.1. [Q61990-1]
NP_001096636.1. NM_001103166.1. [Q61990-2]
NP_035172.2. NM_011042.2. [Q61990-3]
UniGeneiMm.236513.

3D structure databases

ProteinModelPortaliQ61990.
SMRiQ61990.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202043. 12 interactors.
IntActiQ61990. 18 interactors.
MINTiMINT-1520086.
STRINGi10090.ENSMUSP00000076294.

PTM databases

iPTMnetiQ61990.
PhosphoSitePlusiQ61990.

2D gel databases

REPRODUCTION-2DPAGEQ61990.

Proteomic databases

EPDiQ61990.
PaxDbiQ61990.
PeptideAtlasiQ61990.
PRIDEiQ61990.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000077037; ENSMUSP00000076294; ENSMUSG00000056851. [Q61990-1]
ENSMUST00000078404; ENSMUSP00000077509; ENSMUSG00000056851. [Q61990-3]
ENSMUST00000108838; ENSMUSP00000104466; ENSMUSG00000056851. [Q61990-2]
GeneIDi18521.
KEGGimmu:18521.
UCSCiuc007xvy.2. mouse. [Q61990-1]
uc007xvz.2. mouse. [Q61990-2]
uc009vat.1. mouse. [Q61990-3]

Organism-specific databases

CTDi5094.
MGIiMGI:108202. Pcbp2.

Phylogenomic databases

eggNOGiKOG2190. Eukaryota.
ENOG410XNN8. LUCA.
GeneTreeiENSGT00760000119144.
HOGENOMiHOG000182823.
HOVERGENiHBG053520.
InParanoidiQ61990.
KOiK13162.
OMAiCWHELEQ.
OrthoDBiEOG091G0DHI.
PhylomeDBiQ61990.
TreeFamiTF318292.

Enzyme and pathway databases

ReactomeiR-MMU-72163. mRNA Splicing - Major Pathway.
R-MMU-72203. Processing of Capped Intron-Containing Pre-mRNA.

Miscellaneous databases

ChiTaRSiPcbp2. mouse.
PROiQ61990.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000056851.
CleanExiMM_PCBP2.
GenevisibleiQ61990. MM.

Family and domain databases

Gene3Di3.30.1370.10. 3 hits.
InterProiIPR004087. KH_dom.
IPR004088. KH_dom_type_1.
[Graphical view]
PfamiPF00013. KH_1. 3 hits.
[Graphical view]
SMARTiSM00322. KH. 3 hits.
[Graphical view]
SUPFAMiSSF54791. SSF54791. 3 hits.
PROSITEiPS50084. KH_TYPE_1. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPCBP2_MOUSE
AccessioniPrimary (citable) accession number: Q61990
Secondary accession number(s): Q61383, Q62042
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 139 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.