SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q61985

- NF2L1_MOUSE

UniProt

Q61985 - NF2L1_MOUSE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Nuclear factor erythroid 2-related factor 1
Gene
Nfe2l1, Nrf1
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Isoform 3 interacts with the extended kappa 3 site of the TNF alpha promoter after Fc gamma RIII stimulation and participates in the induction of this cytokine. According to 1 Publication, isoform 1 is either inactive or represses the transcriptional activation. Isoform 2 activates the expression of antioxidant response element (ARE) containing reporters and genes in cells, and may play a role in oxidative stress response. According to 1 Publication, isoform 1 also activates ARE-driven genes.2 Publications

GO - Molecular functioni

  1. RNA polymerase II transcription factor binding transcription factor activity involved in positive regulation of transcription Source: MGI
  2. protein binding Source: MGI
  3. sequence-specific DNA binding Source: InterPro
  4. sequence-specific DNA binding transcription factor activity Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. erythrocyte differentiation Source: MGI
  2. positive regulation of transcription from RNA polymerase II promoter Source: MGI
Complete GO annotation...

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear factor erythroid 2-related factor 1
Short name:
NF-E2-related factor 1
Short name:
NFE2-related factor 1
Alternative name(s):
Nuclear factor, erythroid derived 2, like 1
Gene namesi
Name:Nfe2l1
Synonyms:Nrf1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 11

Organism-specific databases

MGIiMGI:99421. Nfe2l1.

Subcellular locationi

Endoplasmic reticulum membrane By similarity; Single-pass type II membrane protein. Nucleus By similarity 1 Publication
Isoform 2 : Cytoplasm. Nucleus 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei7 – 2418Helical; Signal-anchor for type II membrane protein; Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
  3. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 741741Nuclear factor erythroid 2-related factor 1
PRO_0000076448Add
BLAST

Post-translational modificationi

Phosphorylation by CKII could regulate DNA-binding activity.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ61985.

PTM databases

PhosphoSiteiQ61985.

Expressioni

Tissue specificityi

Isoform 2 levels in white fat, lung and liver are increased compared to isoform 1 (at protein level). Isoform 1 and isoform 2 are widely expressed including kidney, brown fat, white fat, large intestine, small intestine, stomach, lung, brain and liver. Isoform 2 levels are elevated in brown fat and brain, but are reduced in liver compared to isoform 1 levels. Isoform 1 and isoform 2 are expressed in mouse embryonic fibroblasts (MEF).1 Publication

Gene expression databases

CleanExiMM_NFE2L1.
MM_NRF1.
GenevestigatoriQ61985.

Interactioni

Subunit structurei

Interacts with KEAP1 By similarity. Interacts with MAFG; this interaction is required for binding to ARE.1 Publication

Structurei

3D structure databases

DisProtiDP00671.
ProteinModelPortaliQ61985.
SMRiQ61985. Positions 581-683.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini623 – 68664bZIP
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni625 – 64420Basic motif By similarity
Add
BLAST
Regioni651 – 66515Leucine-zipper By similarity
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi730 – 7378Nuclear localization signal Reviewed prediction

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi125 – 259135Asp/Glu-rich (acidic)
Add
BLAST
Compositional biasi414 – 44734Asp/Glu-rich (acidic)
Add
BLAST
Compositional biasi467 – 48620Poly-Ser
Add
BLAST

Sequence similaritiesi

Belongs to the bZIP family. CNC subfamily.

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG309753.
GeneTreeiENSGT00550000074399.
HOGENOMiHOG000185844.
HOVERGENiHBG052609.
KOiK09040.
OMAiCAHATAR.

Family and domain databases

Gene3Di1.10.880.10. 1 hit.
InterProiIPR004827. bZIP.
IPR004826. bZIP_Maf.
IPR008917. TF_DNA-bd.
[Graphical view]
PfamiPF03131. bZIP_Maf. 1 hit.
[Graphical view]
SMARTiSM00338. BRLZ. 1 hit.
[Graphical view]
SUPFAMiSSF47454. SSF47454. 1 hit.
PROSITEiPS50217. BZIP. 1 hit.
PS00036. BZIP_BASIC. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative promoter usage and alternative splicing. Align

Isoform 1 (identifier: Q61985-1) [UniParc]FASTAAdd to Basket

Also known as: Long, Nrf1a

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MLSLKKYLTE GLLQFTILLS LIGVRVDVDT YLTSQLPPLR EIILGPSSAY    50
TQTQFHNLRN TLDGYGIHPK SIDLDNYFTA RRLLSQVRAL DRFQVPTTEV 100
NAWLVHRDPE GSVSGSQPNS GLALESSSGL QDVTGPDNGV RESETEQGFG 150
EDLEDLGAVA PPVSGDLTKE DIDLIDILWR QDIDLGAGRE VFDYSHRQKE 200
QDVDKELQDG REREDTWSGE GAEALARDLL VDGETGESFP AQFPADVSSI 250
PEAVPSESES PALQNSLLSP LLTGTESPFD LEQQWQDLMS IMEMQAMEVN 300
TSASEILYNA PPGDPLSTNY SLAPNTPINQ NVSLHQASLG GCSQDFSLFS 350
PEVESLPVAS SSTLLPLVPS NSTSLNSTFG STNLAGLFFP SQLNGTANDT 400
SGPELPDPLG GLLDEAMLDE ISLMDLAIEE GFNPVQASQL EEEFDSDSGL 450
SLDSSHSPSS LSSSEGSSSS SSSSSSSSAS SSASSSFSEE GAVGYSSDSE 500
TLDLEEAEGA VGYQPEYSKF CRMSYQDPSQ LSCLPYLEHV GHNHTYNMAP 550
SALDSADLPP PSTLKKGSKE KQADFLDKQM SRDEHRARAM KIPFTNDKII 600
NLPVEEFNEL LSKYQLSEAQ LSLIRDIRRR GKNKMAAQNC RKRKLDTILN 650
LERDVEDLQR DKARLLREKV EFLRSLRQMK QKVQSLYQEV FGRLRDEHGR 700
PYSPSQYALQ YAGDGSVLLI PRTMADQQAR RQERKPKDRR K 741

Note: Produced by alternative promoter usage.

Length:741
Mass (Da):81,575
Last modified:May 29, 2013 - v2
Checksum:iDDB267F32CD2A92C
GO
Isoform 2 (identifier: Q61985-3) [UniParc]FASTAAdd to Basket

Also known as: Nrf1b

The sequence of this isoform differs from the canonical sequence as follows:
     1-158: Missing.
     159-170: VAPPVSGDLTKE → MGWESRLTAASA

Note: Produced by alternative promoter usage.

Show »
Length:583
Mass (Da):64,269
Checksum:iFA621B98FA01E566
GO
Isoform 3 (identifier: Q61985-2) [UniParc]FASTAAdd to Basket

Also known as: Short

The sequence of this isoform differs from the canonical sequence as follows:
     1-158: Missing.
     159-291: Missing.
     447-583: Missing.

Note: Produced by alternative splicing of isoform 1. No experimental confirmation available.

Show »
Length:313
Mass (Da):35,158
Checksum:iE204C4337A71E365
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 158158Missing in isoform 2 and isoform 3.
VSP_000580Add
BLAST
Alternative sequencei159 – 291133Missing in isoform 3.
VSP_046523Add
BLAST
Alternative sequencei159 – 17012VAPPV…DLTKE → MGWESRLTAASA in isoform 2.
VSP_046524Add
BLAST
Alternative sequencei447 – 583137Missing in isoform 3.
VSP_000581Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti318 – 3181T → S in CAA55362. 1 Publication
Sequence conflicti387 – 3871L → P in CAA55362. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X78709 mRNA. Translation: CAA55362.1.
AF015881 Genomic DNA. Translation: AAC40108.1.
AL596384 Genomic DNA. No translation available.
CCDSiCCDS25304.1. [Q61985-1]
CCDS48894.1. [Q61985-3]
PIRiI48694.
RefSeqiNP_001123922.1. NM_001130450.1. [Q61985-1]
NP_032712.2. NM_008686.3. [Q61985-1]
UniGeneiMm.6743.

Genome annotation databases

EnsembliENSMUST00000081775; ENSMUSP00000080467; ENSMUSG00000038615. [Q61985-1]
ENSMUST00000107657; ENSMUSP00000103284; ENSMUSG00000038615. [Q61985-1]
ENSMUST00000107658; ENSMUSP00000103285; ENSMUSG00000038615. [Q61985-1]
ENSMUST00000167110; ENSMUSP00000127804; ENSMUSG00000038615. [Q61985-3]
ENSMUST00000167149; ENSMUSP00000128527; ENSMUSG00000038615. [Q61985-1]
GeneIDi18023.
KEGGimmu:18023.

Keywords - Coding sequence diversityi

Alternative promoter usage, Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X78709 mRNA. Translation: CAA55362.1 .
AF015881 Genomic DNA. Translation: AAC40108.1 .
AL596384 Genomic DNA. No translation available.
CCDSi CCDS25304.1. [Q61985-1 ]
CCDS48894.1. [Q61985-3 ]
PIRi I48694.
RefSeqi NP_001123922.1. NM_001130450.1. [Q61985-1 ]
NP_032712.2. NM_008686.3. [Q61985-1 ]
UniGenei Mm.6743.

3D structure databases

DisProti DP00671.
ProteinModelPortali Q61985.
SMRi Q61985. Positions 581-683.
ModBasei Search...

PTM databases

PhosphoSitei Q61985.

Proteomic databases

PRIDEi Q61985.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000081775 ; ENSMUSP00000080467 ; ENSMUSG00000038615 . [Q61985-1 ]
ENSMUST00000107657 ; ENSMUSP00000103284 ; ENSMUSG00000038615 . [Q61985-1 ]
ENSMUST00000107658 ; ENSMUSP00000103285 ; ENSMUSG00000038615 . [Q61985-1 ]
ENSMUST00000167110 ; ENSMUSP00000127804 ; ENSMUSG00000038615 . [Q61985-3 ]
ENSMUST00000167149 ; ENSMUSP00000128527 ; ENSMUSG00000038615 . [Q61985-1 ]
GeneIDi 18023.
KEGGi mmu:18023.

Organism-specific databases

CTDi 4779.
MGIi MGI:99421. Nfe2l1.

Phylogenomic databases

eggNOGi NOG309753.
GeneTreei ENSGT00550000074399.
HOGENOMi HOG000185844.
HOVERGENi HBG052609.
KOi K09040.
OMAi CAHATAR.

Miscellaneous databases

ChiTaRSi NFE2L1. mouse.
NextBioi 293083.
PROi Q61985.
SOURCEi Search...

Gene expression databases

CleanExi MM_NFE2L1.
MM_NRF1.
Genevestigatori Q61985.

Family and domain databases

Gene3Di 1.10.880.10. 1 hit.
InterProi IPR004827. bZIP.
IPR004826. bZIP_Maf.
IPR008917. TF_DNA-bd.
[Graphical view ]
Pfami PF03131. bZIP_Maf. 1 hit.
[Graphical view ]
SMARTi SM00338. BRLZ. 1 hit.
[Graphical view ]
SUPFAMi SSF47454. SSF47454. 1 hit.
PROSITEi PS50217. BZIP. 1 hit.
PS00036. BZIP_BASIC. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Embryo.
  2. "A novel splice variant of the transcription factor Nrf1 interacts with the TNFalpha promoter and stimulates transcription."
    Prieschl E.E., Novotny V., Csonga R., Jaksche D., Elbe-Buerger A., Thumb W., Auer M., Stingl G., Baumruker T.
    Nucleic Acids Res. 26:2291-2297(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 3), FUNCTION, PHOSPHORYLATION BY CKII.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "Characterization of Nrf1b, a novel isoform of the nuclear factor-erythroid-2 related transcription factor-1 that activates antioxidant response element-regulated genes."
    Kwong E.K., Kim K.M., Penalosa P.J., Chan J.Y.
    PLoS ONE 7:E48404-E48404(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: ALTERNATIVE PROMOTER USAGE, IDENTIFICATION OF ISOFORM 2, FUNCTION, INTERACTION WITH MAFG, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiNF2L1_MOUSE
AccessioniPrimary (citable) accession number: Q61985
Secondary accession number(s): E9Q038, O70234
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 29, 2013
Last modified: July 9, 2014
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi