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Q61985

- NF2L1_MOUSE

UniProt

Q61985 - NF2L1_MOUSE

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Protein

Nuclear factor erythroid 2-related factor 1

Gene

Nfe2l1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Isoform 3 interacts with the extended kappa 3 site of the TNF alpha promoter after Fc gamma RIII stimulation and participates in the induction of this cytokine. According to PubMed:9580677, isoform 1 is either inactive or represses the transcriptional activation. Isoform 2 activates the expression of antioxidant response element (ARE) containing reporters and genes in cells, and may play a role in oxidative stress response. According to PubMed:23144760, isoform 1 also activates ARE-driven genes.2 Publications

GO - Molecular functioni

  1. RNA polymerase II distal enhancer sequence-specific DNA binding Source: NTNU_SB
  2. RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: NTNU_SB
  3. RNA polymerase II transcription factor binding transcription factor activity involved in positive regulation of transcription Source: MGI

GO - Biological processi

  1. erythrocyte differentiation Source: MGI
  2. positive regulation of transcription from RNA polymerase II promoter Source: NTNU_SB
Complete GO annotation...

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear factor erythroid 2-related factor 1
Short name:
NF-E2-related factor 1
Short name:
NFE2-related factor 1
Alternative name(s):
Nuclear factor, erythroid derived 2, like 1
Gene namesi
Name:Nfe2l1
Synonyms:Nrf1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 11

Organism-specific databases

MGIiMGI:99421. Nfe2l1.

Subcellular locationi

Endoplasmic reticulum membrane By similarity; Single-pass type II membrane protein 1 Publication. Nucleus PROSITE-ProRule annotation

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB-KW
  2. integral component of membrane Source: UniProtKB-KW
  3. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 741741Nuclear factor erythroid 2-related factor 1PRO_0000076448Add
BLAST

Post-translational modificationi

Phosphorylation by CKII could regulate DNA-binding activity.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ61985.
PRIDEiQ61985.

PTM databases

PhosphoSiteiQ61985.

Expressioni

Tissue specificityi

Isoform 2 levels in white fat, lung and liver are increased compared to isoform 1 (at protein level). Isoform 1 and isoform 2 are widely expressed including kidney, brown fat, white fat, large intestine, small intestine, stomach, lung, brain and liver. Isoform 2 levels are elevated in brown fat and brain, but are reduced in liver compared to isoform 1 levels. Isoform 1 and isoform 2 are expressed in mouse embryonic fibroblasts (MEF).1 Publication

Gene expression databases

CleanExiMM_NFE2L1.
MM_NRF1.
ExpressionAtlasiQ61985. baseline and differential.
GenevestigatoriQ61985.

Interactioni

Subunit structurei

Interacts with KEAP1 (By similarity). Interacts with MAFG; this interaction is required for binding to ARE.By similarity1 Publication

Structurei

3D structure databases

DisProtiDP00671.
ProteinModelPortaliQ61985.
SMRiQ61985. Positions 581-683.
ModBaseiSearch...
MobiDBiSearch...

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei7 – 2418Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini623 – 68664bZIPPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni625 – 64420Basic motifPROSITE-ProRule annotationAdd
BLAST
Regioni651 – 66515Leucine-zipperPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi730 – 7378Nuclear localization signalSequence Analysis

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi125 – 259135Asp/Glu-rich (acidic)Add
BLAST
Compositional biasi414 – 44734Asp/Glu-rich (acidic)Add
BLAST
Compositional biasi467 – 48620Poly-SerAdd
BLAST

Sequence similaritiesi

Belongs to the bZIP family. CNC subfamily.Curated
Contains 1 bZIP (basic-leucine zipper) domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG309753.
GeneTreeiENSGT00550000074399.
HOGENOMiHOG000185844.
HOVERGENiHBG052609.
InParanoidiQ61985.
KOiK09040.
OMAiCAHATAR.

Family and domain databases

Gene3Di1.10.880.10. 1 hit.
InterProiIPR004827. bZIP.
IPR004826. bZIP_Maf.
IPR008917. TF_DNA-bd.
[Graphical view]
PfamiPF03131. bZIP_Maf. 1 hit.
[Graphical view]
SMARTiSM00338. BRLZ. 1 hit.
[Graphical view]
SUPFAMiSSF47454. SSF47454. 1 hit.
PROSITEiPS50217. BZIP. 1 hit.
PS00036. BZIP_BASIC. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative promoter usage and alternative splicing. Align

Isoform 1 (identifier: Q61985) [UniParc]FASTAAdd to Basket

Also known as: Long, Nrf1a

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLSLKKYLTE GLLQFTILLS LIGVRVDVDT YLTSQLPPLR EIILGPSSAY
60 70 80 90 100
TQTQFHNLRN TLDGYGIHPK SIDLDNYFTA RRLLSQVRAL DRFQVPTTEV
110 120 130 140 150
NAWLVHRDPE GSVSGSQPNS GLALESSSGL QDVTGPDNGV RESETEQGFG
160 170 180 190 200
EDLEDLGAVA PPVSGDLTKE DIDLIDILWR QDIDLGAGRE VFDYSHRQKE
210 220 230 240 250
QDVDKELQDG REREDTWSGE GAEALARDLL VDGETGESFP AQFPADVSSI
260 270 280 290 300
PEAVPSESES PALQNSLLSP LLTGTESPFD LEQQWQDLMS IMEMQAMEVN
310 320 330 340 350
TSASEILYNA PPGDPLSTNY SLAPNTPINQ NVSLHQASLG GCSQDFSLFS
360 370 380 390 400
PEVESLPVAS SSTLLPLVPS NSTSLNSTFG STNLAGLFFP SQLNGTANDT
410 420 430 440 450
SGPELPDPLG GLLDEAMLDE ISLMDLAIEE GFNPVQASQL EEEFDSDSGL
460 470 480 490 500
SLDSSHSPSS LSSSEGSSSS SSSSSSSSAS SSASSSFSEE GAVGYSSDSE
510 520 530 540 550
TLDLEEAEGA VGYQPEYSKF CRMSYQDPSQ LSCLPYLEHV GHNHTYNMAP
560 570 580 590 600
SALDSADLPP PSTLKKGSKE KQADFLDKQM SRDEHRARAM KIPFTNDKII
610 620 630 640 650
NLPVEEFNEL LSKYQLSEAQ LSLIRDIRRR GKNKMAAQNC RKRKLDTILN
660 670 680 690 700
LERDVEDLQR DKARLLREKV EFLRSLRQMK QKVQSLYQEV FGRLRDEHGR
710 720 730 740
PYSPSQYALQ YAGDGSVLLI PRTMADQQAR RQERKPKDRR K

Note: Produced by alternative promoter usage.

Length:741
Mass (Da):81,575
Last modified:May 29, 2013 - v2
Checksum:iDDB267F32CD2A92C
GO
Isoform 2 (identifier: Q61985-3) [UniParc]FASTAAdd to Basket

Also known as: Nrf1b

The sequence of this isoform differs from the canonical sequence as follows:
     1-158: Missing.
     159-170: VAPPVSGDLTKE → MGWESRLTAASA

Note: Produced by alternative promoter usage.

Show »
Length:583
Mass (Da):64,269
Checksum:iFA621B98FA01E566
GO
Isoform 3 (identifier: Q61985-2) [UniParc]FASTAAdd to Basket

Also known as: Short

The sequence of this isoform differs from the canonical sequence as follows:
     1-158: Missing.
     159-291: Missing.
     447-583: Missing.

Note: Produced by alternative splicing of isoform 1. No experimental confirmation available.

Show »
Length:313
Mass (Da):35,158
Checksum:iE204C4337A71E365
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti318 – 3181T → S in CAA55362. (PubMed:7759107)Curated
Sequence conflicti387 – 3871L → P in CAA55362. (PubMed:7759107)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 158158Missing in isoform 2 and isoform 3. CuratedVSP_000580Add
BLAST
Alternative sequencei159 – 291133Missing in isoform 3. CuratedVSP_046523Add
BLAST
Alternative sequencei159 – 17012VAPPV…DLTKE → MGWESRLTAASA in isoform 2. CuratedVSP_046524Add
BLAST
Alternative sequencei447 – 583137Missing in isoform 3. CuratedVSP_000581Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X78709 mRNA. Translation: CAA55362.1.
AF015881 Genomic DNA. Translation: AAC40108.1.
AL596384 Genomic DNA. No translation available.
CCDSiCCDS25304.1. [Q61985-1]
CCDS48894.1. [Q61985-3]
PIRiI48694.
RefSeqiNP_001123922.1. NM_001130450.1. [Q61985-1]
NP_032712.2. NM_008686.3. [Q61985-1]
UniGeneiMm.6743.

Genome annotation databases

EnsembliENSMUST00000081775; ENSMUSP00000080467; ENSMUSG00000038615. [Q61985-1]
ENSMUST00000107657; ENSMUSP00000103284; ENSMUSG00000038615. [Q61985-1]
ENSMUST00000107658; ENSMUSP00000103285; ENSMUSG00000038615. [Q61985-1]
ENSMUST00000167110; ENSMUSP00000127804; ENSMUSG00000038615. [Q61985-3]
ENSMUST00000167149; ENSMUSP00000128527; ENSMUSG00000038615. [Q61985-1]
GeneIDi18023.
KEGGimmu:18023.

Keywords - Coding sequence diversityi

Alternative promoter usage, Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X78709 mRNA. Translation: CAA55362.1 .
AF015881 Genomic DNA. Translation: AAC40108.1 .
AL596384 Genomic DNA. No translation available.
CCDSi CCDS25304.1. [Q61985-1 ]
CCDS48894.1. [Q61985-3 ]
PIRi I48694.
RefSeqi NP_001123922.1. NM_001130450.1. [Q61985-1 ]
NP_032712.2. NM_008686.3. [Q61985-1 ]
UniGenei Mm.6743.

3D structure databases

DisProti DP00671.
ProteinModelPortali Q61985.
SMRi Q61985. Positions 581-683.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei Q61985.

Proteomic databases

MaxQBi Q61985.
PRIDEi Q61985.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000081775 ; ENSMUSP00000080467 ; ENSMUSG00000038615 . [Q61985-1 ]
ENSMUST00000107657 ; ENSMUSP00000103284 ; ENSMUSG00000038615 . [Q61985-1 ]
ENSMUST00000107658 ; ENSMUSP00000103285 ; ENSMUSG00000038615 . [Q61985-1 ]
ENSMUST00000167110 ; ENSMUSP00000127804 ; ENSMUSG00000038615 . [Q61985-3 ]
ENSMUST00000167149 ; ENSMUSP00000128527 ; ENSMUSG00000038615 . [Q61985-1 ]
GeneIDi 18023.
KEGGi mmu:18023.

Organism-specific databases

CTDi 4779.
MGIi MGI:99421. Nfe2l1.

Phylogenomic databases

eggNOGi NOG309753.
GeneTreei ENSGT00550000074399.
HOGENOMi HOG000185844.
HOVERGENi HBG052609.
InParanoidi Q61985.
KOi K09040.
OMAi CAHATAR.

Miscellaneous databases

ChiTaRSi NFE2L1. mouse.
NextBioi 293083.
PROi Q61985.
SOURCEi Search...

Gene expression databases

CleanExi MM_NFE2L1.
MM_NRF1.
ExpressionAtlasi Q61985. baseline and differential.
Genevestigatori Q61985.

Family and domain databases

Gene3Di 1.10.880.10. 1 hit.
InterProi IPR004827. bZIP.
IPR004826. bZIP_Maf.
IPR008917. TF_DNA-bd.
[Graphical view ]
Pfami PF03131. bZIP_Maf. 1 hit.
[Graphical view ]
SMARTi SM00338. BRLZ. 1 hit.
[Graphical view ]
SUPFAMi SSF47454. SSF47454. 1 hit.
PROSITEi PS50217. BZIP. 1 hit.
PS00036. BZIP_BASIC. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Embryo.
  2. "A novel splice variant of the transcription factor Nrf1 interacts with the TNFalpha promoter and stimulates transcription."
    Prieschl E.E., Novotny V., Csonga R., Jaksche D., Elbe-Buerger A., Thumb W., Auer M., Stingl G., Baumruker T.
    Nucleic Acids Res. 26:2291-2297(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 3), FUNCTION, PHOSPHORYLATION BY CKII.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "Characterization of Nrf1b, a novel isoform of the nuclear factor-erythroid-2 related transcription factor-1 that activates antioxidant response element-regulated genes."
    Kwong E.K., Kim K.M., Penalosa P.J., Chan J.Y.
    PLoS ONE 7:E48404-E48404(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: ALTERNATIVE PROMOTER USAGE, IDENTIFICATION OF ISOFORM 2, FUNCTION, INTERACTION WITH MAFG, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiNF2L1_MOUSE
AccessioniPrimary (citable) accession number: Q61985
Secondary accession number(s): E9Q038, O70234
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 29, 2013
Last modified: October 29, 2014
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3