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Q61985

- NF2L1_MOUSE

UniProt

Q61985 - NF2L1_MOUSE

Protein

Nuclear factor erythroid 2-related factor 1

Gene

Nfe2l1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 124 (01 Oct 2014)
      Sequence version 2 (29 May 2013)
      Previous versions | rss
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    Functioni

    Isoform 3 interacts with the extended kappa 3 site of the TNF alpha promoter after Fc gamma RIII stimulation and participates in the induction of this cytokine. According to PubMed:9580677, isoform 1 is either inactive or represses the transcriptional activation. Isoform 2 activates the expression of antioxidant response element (ARE) containing reporters and genes in cells, and may play a role in oxidative stress response. According to PubMed:23144760, isoform 1 also activates ARE-driven genes.2 Publications

    GO - Molecular functioni

    1. protein binding Source: MGI
    2. RNA polymerase II distal enhancer sequence-specific DNA binding Source: NTNU_SB
    3. RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: NTNU_SB
    4. RNA polymerase II transcription factor binding transcription factor activity involved in positive regulation of transcription Source: MGI

    GO - Biological processi

    1. erythrocyte differentiation Source: MGI
    2. positive regulation of transcription from RNA polymerase II promoter Source: NTNU_SB

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nuclear factor erythroid 2-related factor 1
    Short name:
    NF-E2-related factor 1
    Short name:
    NFE2-related factor 1
    Alternative name(s):
    Nuclear factor, erythroid derived 2, like 1
    Gene namesi
    Name:Nfe2l1
    Synonyms:Nrf1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 11

    Organism-specific databases

    MGIiMGI:99421. Nfe2l1.

    Subcellular locationi

    Endoplasmic reticulum membrane By similarity; Single-pass type II membrane protein 1 Publication. Nucleus PROSITE-ProRule annotation

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW
    3. nucleus Source: MGI

    Keywords - Cellular componenti

    Cytoplasm, Endoplasmic reticulum, Membrane, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 741741Nuclear factor erythroid 2-related factor 1PRO_0000076448Add
    BLAST

    Post-translational modificationi

    Phosphorylation by CKII could regulate DNA-binding activity.1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiQ61985.

    PTM databases

    PhosphoSiteiQ61985.

    Expressioni

    Tissue specificityi

    Isoform 2 levels in white fat, lung and liver are increased compared to isoform 1 (at protein level). Isoform 1 and isoform 2 are widely expressed including kidney, brown fat, white fat, large intestine, small intestine, stomach, lung, brain and liver. Isoform 2 levels are elevated in brown fat and brain, but are reduced in liver compared to isoform 1 levels. Isoform 1 and isoform 2 are expressed in mouse embryonic fibroblasts (MEF).1 Publication

    Gene expression databases

    CleanExiMM_NFE2L1.
    MM_NRF1.
    GenevestigatoriQ61985.

    Interactioni

    Subunit structurei

    Interacts with KEAP1 By similarity. Interacts with MAFG; this interaction is required for binding to ARE.By similarity1 Publication

    Structurei

    3D structure databases

    DisProtiDP00671.
    ProteinModelPortaliQ61985.
    SMRiQ61985. Positions 581-683.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei7 – 2418Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini623 – 68664bZIPPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni625 – 64420Basic motifPROSITE-ProRule annotationAdd
    BLAST
    Regioni651 – 66515Leucine-zipperPROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi730 – 7378Nuclear localization signalSequence Analysis

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi125 – 259135Asp/Glu-rich (acidic)Add
    BLAST
    Compositional biasi414 – 44734Asp/Glu-rich (acidic)Add
    BLAST
    Compositional biasi467 – 48620Poly-SerAdd
    BLAST

    Sequence similaritiesi

    Belongs to the bZIP family. CNC subfamily.Curated
    Contains 1 bZIP (basic-leucine zipper) domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG309753.
    GeneTreeiENSGT00550000074399.
    HOGENOMiHOG000185844.
    HOVERGENiHBG052609.
    KOiK09040.
    OMAiCAHATAR.

    Family and domain databases

    Gene3Di1.10.880.10. 1 hit.
    InterProiIPR004827. bZIP.
    IPR004826. bZIP_Maf.
    IPR008917. TF_DNA-bd.
    [Graphical view]
    PfamiPF03131. bZIP_Maf. 1 hit.
    [Graphical view]
    SMARTiSM00338. BRLZ. 1 hit.
    [Graphical view]
    SUPFAMiSSF47454. SSF47454. 1 hit.
    PROSITEiPS50217. BZIP. 1 hit.
    PS00036. BZIP_BASIC. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative promoter usage and alternative splicing. Align

    Isoform 1 (identifier: Q61985-1) [UniParc]FASTAAdd to Basket

    Also known as: Long, Nrf1a

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MLSLKKYLTE GLLQFTILLS LIGVRVDVDT YLTSQLPPLR EIILGPSSAY    50
    TQTQFHNLRN TLDGYGIHPK SIDLDNYFTA RRLLSQVRAL DRFQVPTTEV 100
    NAWLVHRDPE GSVSGSQPNS GLALESSSGL QDVTGPDNGV RESETEQGFG 150
    EDLEDLGAVA PPVSGDLTKE DIDLIDILWR QDIDLGAGRE VFDYSHRQKE 200
    QDVDKELQDG REREDTWSGE GAEALARDLL VDGETGESFP AQFPADVSSI 250
    PEAVPSESES PALQNSLLSP LLTGTESPFD LEQQWQDLMS IMEMQAMEVN 300
    TSASEILYNA PPGDPLSTNY SLAPNTPINQ NVSLHQASLG GCSQDFSLFS 350
    PEVESLPVAS SSTLLPLVPS NSTSLNSTFG STNLAGLFFP SQLNGTANDT 400
    SGPELPDPLG GLLDEAMLDE ISLMDLAIEE GFNPVQASQL EEEFDSDSGL 450
    SLDSSHSPSS LSSSEGSSSS SSSSSSSSAS SSASSSFSEE GAVGYSSDSE 500
    TLDLEEAEGA VGYQPEYSKF CRMSYQDPSQ LSCLPYLEHV GHNHTYNMAP 550
    SALDSADLPP PSTLKKGSKE KQADFLDKQM SRDEHRARAM KIPFTNDKII 600
    NLPVEEFNEL LSKYQLSEAQ LSLIRDIRRR GKNKMAAQNC RKRKLDTILN 650
    LERDVEDLQR DKARLLREKV EFLRSLRQMK QKVQSLYQEV FGRLRDEHGR 700
    PYSPSQYALQ YAGDGSVLLI PRTMADQQAR RQERKPKDRR K 741

    Note: Produced by alternative promoter usage.

    Length:741
    Mass (Da):81,575
    Last modified:May 29, 2013 - v2
    Checksum:iDDB267F32CD2A92C
    GO
    Isoform 2 (identifier: Q61985-3) [UniParc]FASTAAdd to Basket

    Also known as: Nrf1b

    The sequence of this isoform differs from the canonical sequence as follows:
         1-158: Missing.
         159-170: VAPPVSGDLTKE → MGWESRLTAASA

    Note: Produced by alternative promoter usage.

    Show »
    Length:583
    Mass (Da):64,269
    Checksum:iFA621B98FA01E566
    GO
    Isoform 3 (identifier: Q61985-2) [UniParc]FASTAAdd to Basket

    Also known as: Short

    The sequence of this isoform differs from the canonical sequence as follows:
         1-158: Missing.
         159-291: Missing.
         447-583: Missing.

    Note: Produced by alternative splicing of isoform 1. No experimental confirmation available.

    Show »
    Length:313
    Mass (Da):35,158
    Checksum:iE204C4337A71E365
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti318 – 3181T → S in CAA55362. (PubMed:7759107)Curated
    Sequence conflicti387 – 3871L → P in CAA55362. (PubMed:7759107)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 158158Missing in isoform 2 and isoform 3. CuratedVSP_000580Add
    BLAST
    Alternative sequencei159 – 291133Missing in isoform 3. CuratedVSP_046523Add
    BLAST
    Alternative sequencei159 – 17012VAPPV…DLTKE → MGWESRLTAASA in isoform 2. CuratedVSP_046524Add
    BLAST
    Alternative sequencei447 – 583137Missing in isoform 3. CuratedVSP_000581Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X78709 mRNA. Translation: CAA55362.1.
    AF015881 Genomic DNA. Translation: AAC40108.1.
    AL596384 Genomic DNA. No translation available.
    CCDSiCCDS25304.1. [Q61985-1]
    CCDS48894.1. [Q61985-3]
    PIRiI48694.
    RefSeqiNP_001123922.1. NM_001130450.1. [Q61985-1]
    NP_032712.2. NM_008686.3. [Q61985-1]
    UniGeneiMm.6743.

    Genome annotation databases

    EnsembliENSMUST00000081775; ENSMUSP00000080467; ENSMUSG00000038615. [Q61985-1]
    ENSMUST00000107657; ENSMUSP00000103284; ENSMUSG00000038615. [Q61985-1]
    ENSMUST00000107658; ENSMUSP00000103285; ENSMUSG00000038615. [Q61985-1]
    ENSMUST00000167110; ENSMUSP00000127804; ENSMUSG00000038615. [Q61985-3]
    ENSMUST00000167149; ENSMUSP00000128527; ENSMUSG00000038615. [Q61985-1]
    GeneIDi18023.
    KEGGimmu:18023.

    Keywords - Coding sequence diversityi

    Alternative promoter usage, Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X78709 mRNA. Translation: CAA55362.1 .
    AF015881 Genomic DNA. Translation: AAC40108.1 .
    AL596384 Genomic DNA. No translation available.
    CCDSi CCDS25304.1. [Q61985-1 ]
    CCDS48894.1. [Q61985-3 ]
    PIRi I48694.
    RefSeqi NP_001123922.1. NM_001130450.1. [Q61985-1 ]
    NP_032712.2. NM_008686.3. [Q61985-1 ]
    UniGenei Mm.6743.

    3D structure databases

    DisProti DP00671.
    ProteinModelPortali Q61985.
    SMRi Q61985. Positions 581-683.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q61985.

    Proteomic databases

    PRIDEi Q61985.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000081775 ; ENSMUSP00000080467 ; ENSMUSG00000038615 . [Q61985-1 ]
    ENSMUST00000107657 ; ENSMUSP00000103284 ; ENSMUSG00000038615 . [Q61985-1 ]
    ENSMUST00000107658 ; ENSMUSP00000103285 ; ENSMUSG00000038615 . [Q61985-1 ]
    ENSMUST00000167110 ; ENSMUSP00000127804 ; ENSMUSG00000038615 . [Q61985-3 ]
    ENSMUST00000167149 ; ENSMUSP00000128527 ; ENSMUSG00000038615 . [Q61985-1 ]
    GeneIDi 18023.
    KEGGi mmu:18023.

    Organism-specific databases

    CTDi 4779.
    MGIi MGI:99421. Nfe2l1.

    Phylogenomic databases

    eggNOGi NOG309753.
    GeneTreei ENSGT00550000074399.
    HOGENOMi HOG000185844.
    HOVERGENi HBG052609.
    KOi K09040.
    OMAi CAHATAR.

    Miscellaneous databases

    ChiTaRSi NFE2L1. mouse.
    NextBioi 293083.
    PROi Q61985.
    SOURCEi Search...

    Gene expression databases

    CleanExi MM_NFE2L1.
    MM_NRF1.
    Genevestigatori Q61985.

    Family and domain databases

    Gene3Di 1.10.880.10. 1 hit.
    InterProi IPR004827. bZIP.
    IPR004826. bZIP_Maf.
    IPR008917. TF_DNA-bd.
    [Graphical view ]
    Pfami PF03131. bZIP_Maf. 1 hit.
    [Graphical view ]
    SMARTi SM00338. BRLZ. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47454. SSF47454. 1 hit.
    PROSITEi PS50217. BZIP. 1 hit.
    PS00036. BZIP_BASIC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Tissue: Embryo.
    2. "A novel splice variant of the transcription factor Nrf1 interacts with the TNFalpha promoter and stimulates transcription."
      Prieschl E.E., Novotny V., Csonga R., Jaksche D., Elbe-Buerger A., Thumb W., Auer M., Stingl G., Baumruker T.
      Nucleic Acids Res. 26:2291-2297(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 3), FUNCTION, PHOSPHORYLATION BY CKII.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    4. "Characterization of Nrf1b, a novel isoform of the nuclear factor-erythroid-2 related transcription factor-1 that activates antioxidant response element-regulated genes."
      Kwong E.K., Kim K.M., Penalosa P.J., Chan J.Y.
      PLoS ONE 7:E48404-E48404(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: ALTERNATIVE PROMOTER USAGE, IDENTIFICATION OF ISOFORM 2, FUNCTION, INTERACTION WITH MAFG, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiNF2L1_MOUSE
    AccessioniPrimary (citable) accession number: Q61985
    Secondary accession number(s): E9Q038, O70234
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: May 29, 2013
    Last modified: October 1, 2014
    This is version 124 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3