NAD(P) transhydrogenase, mitochondrial precursor

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Reviewed, UniProtKB/Swiss-Prot Q61941 (NNTM_MOUSE)

Last modified October 13, 2009. Version 78. History...

Clusters with 100%, 90%, 50% identity |

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    NAD(P) transhydrogenase, mitochondrial
    EC=1.6.1.2
Alternative name(s):
    Nicotinamide nucleotide transhydrogenase
    Pyridine nucleotide transhydrogenase

Gene names

Name:

Nnt

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

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Protein attributes

Sequence length	1086 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane.
Catalytic activity	NADPH + NAD⁺ = NADP⁺ + NADH.
Subunit structure	Homodimer By similarity.
Subcellular location	Mitochondrion inner membrane; Multi-pass membrane protein; Matrix side Potential.
Sequence similarities	In the N-terminal section; belongs to the AlaDH/PNT family. In the C-terminal section; belongs to the PNT beta subunit family.

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Ontologies

Keywords
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide Transmembrane
Ligand	NAD NADP
Molecular function	Oxidoreductase
PTM	Acetylation
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW proton transport Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial inner membrane Inferred from direct assay. Source: MGI
Molecular function	NAD(P)+ transhydrogenase (AB-specific) activity Inferred from electronic annotation. Source: EC binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – 43

Mitochondrion By similarity

Chain

44 – 1086

1043

NAD(P) transhydrogenase, mitochondrial

PRO_0000001056

Regions

Topological domain

44 – 474

431

Mitochondrial matrix

Transmembrane

475 – 493

Potential

Transmembrane

501 – 521

Potential

Transmembrane

527 – 546

Potential

Transmembrane

558 – 578

Potential

Topological domain

579 – 595

Mitochondrial matrix

Transmembrane

596 – 616

Potential

Transmembrane

622 – 642

Potential

Transmembrane

646 – 666

Potential

Transmembrane

672 – 691

Potential

Transmembrane

702 – 722

Potential

Topological domain

723 – 739

Cytoplasmic

Transmembrane

740 – 760

Potential

Transmembrane

778 – 797

Potential

Transmembrane

801 – 819

Potential

Transmembrane

833 – 853

Potential

Transmembrane

857 – 879

Potential

Topological domain

880 – 1086

207

Mitochondrial matrix

Nucleotide binding

229 – 259

NAD By similarity

Amino acid modifications

Modified residue

N6-acetyllysine By similarity

Modified residue

100

N6-acetyllysine By similarity

Modified residue

331

N6-acetyllysine By similarity

Modified residue

397

N6-acetyllysine By similarity

Experimental info

Sequence conflict

T → M in CAA89065. Ref.1

Sequence conflict

775

P → L in CAA89065. Ref.1

Sequence conflict

813

G → A in CAA89065. Ref.1

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Sequences

Sequence

Length

Mass (Da)

Tools

Q61941-1 [UniParc].

Last modified August 16, 2004. Version 2.
Checksum: A023285169834D07
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FASTA

1,086

113,838

        10         20         30         40         50         60 
MAHLLKTVVA GCSCPFLSNL GSSKVLPGKR DFVRTLRTHQ ALWCKSPVKP GIPYKQLTVG 

        70         80         90        100        110        120 
VPKEIFQNEK RVALSPAGVQ ALVKQGFNVV VESGAGEASK FPDDLYRAAG AQIQGMKEVL 

       130        140        150        160        170        180 
ASDLVVKVRA PMVNPTLGAH EADFLKPSGT LISFIYPAQN PDLLNKLSER KTTVLAMDQV 

       190        200        210        220        230        240 
PRVTIAQGYD ALSSMANISG YKAVVLAANH FGRFFTGQIT AAGKVPPAKI LIVGGGVAGL 

       250        260        270        280        290        300 
ASAGAAKSMG AVVRGFDTRA AALEQFKSLG AEPLEVDLKE SGEGQGGYAK EMSKEFIEAE 

       310        320        330        340        350        360 
MKLFAQQCKE VDILISTALI PGKKAPVLFS KEMIESMKEG SVVVDLAAEA GGNFETTKPG 

       370        380        390        400        410        420 
ELYVHKGITH IGYTDLPSRM ATQASTLYSN NITKLLKAIS PDKDNFHFEV KDDFDFGTMS 

       430        440        450        460        470        480 
HVIRGTVVMK DGKVIFPAPT PKNIPEEAPV KPKTVAELEA EKAGTVSMYT KTLTTASVYS 

       490        500        510        520        530        540 
AGLTGMLGLG IVAPNVAFSQ MVTTFGLAGI IGYHTVWGVT PALHSPLMSV TNAISGLTAV 

       550        560        570        580        590        600 
GGLALMGGHF YPSTTSQSLA ALATFISSVN IAGGFLVTQR MLDMFKRPTD PPEYNYLYLL 

       610        620        630        640        650        660 
PGGTFVGGYL AALYGGYNIE EIMYLGSGLC CVGALGGLST QGTARLGNAL GMIGVAGGLA 

       670        680        690        700        710        720 
ATLGGLKPDP QLLAQMSGAM AMGGTIGLTI AKRIQISDLP QLVAAFHSLV GLAAVLTCMA 

       730        740        750        760        770        780 
EYIVEYPHFA MDATSNFTKI VAYLGTYIGG VTFSGSLVAY GKLQGILKSA PLLLPGRHAL 

       790        800        810        820        830        840 
NAGLLAASVG GIIPFMADPS FTTGITCLGS VSGLSTLMGV TLTAAIGGAD MPVVITVLNS 

       850        860        870        880        890        900 
YSGWALCAEG FLLNNNLLTI VGALIGSSGA ILSYIMCVAM NRSLANVILG GYGTTSTAGG 

       910        920        930        940        950        960 
KPMEISGTHT EINLDNAVEM IREANSIVIT PGYGLCAAKA QYPIADLVKM LTEQGKKVRF 

       970        980        990       1000       1010       1020 
GIHPVAGRMP GQLNVLLAEA GVPYDIVLEM DEINSDFPDT DLVLVIGAND TVNSAAQEDP 

      1030       1040       1050       1060       1070       1080 
NSIIAGMPVL EVWKSKQVIV MKRSLGVGYA AVDNPIFYKP NTAMLLGDAK KTCDALQAKV 


RESYQK

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References

[1]	"The cDNA sequence of proton-pumping nicotinamide nucleotide transhydrogenase from man and mouse." Lagberg E.M., Betsholtz C., Rydstrom J. Biochim. Biophys. Acta 1273:203-205(1996) [PubMed: 8616157] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6 X CBA. Tissue: Liver.
[2]	"Characterization of a nicotinamide nucleotide transhydrogenase gene from the green alga Acetabularia acetabulum and comparison of its structure with those of the corresponding genes in mouse and Caenorhabditis elegans." Arkblad E.L., Betsholtz C., Mandoli D., Rydstrom J. Biochim. Biophys. Acta 1520:115-123(2001) [PubMed: 11513952] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129/SvJ.
[3]	Lubec G., Kang S.U. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 72-84; 214-224; 268-279; 367-379; 434-442; 769-777 AND 940-949, MASS SPECTROMETRY. Strain: C57BL/6. Tissue: Brain.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
	Z49204 mRNA. Translation: CAA89065.1. AF257157 AF257156 Genomic DNA. Translation: AAF72982.2.
IPI	IPI00323166.
PIR	S54876.
UniGene	Mm.195803
3D structure databases
HSSP	HSSP built from PDB template 1DJL based on UniProtKB Q13423.
SMR	Q61941. Positions 902-1083.
ModBase	Search...
Protein-protein interaction databases
STRING	Q61941.
PTM databases
PhosphoSite	Q61941.
Proteomic databases
PRIDE	Q61941.
Organism-specific databases
MGI	MGI:109279. Nnt.
Phylogenomic databases
HOVERGEN	Q61941.
Enzyme and pathway databases
BRENDA	1.6.1.2. 244.
Gene expression databases
CleanEx	MM_NNT.
Genevestigator	Q61941.
GermOnline	ENSMUSG00000025453. Mus musculus.
Family and domain databases
InterPro	IPR007698. Ala_DH/PNT_C. IPR008142. Ala_DH/PNT_CS1. IPR008143. Ala_DH/PNT_CS2. IPR007886. Ala_DH/PNT_N. IPR016040. NAD(P)-bd_dom. IPR004571. NADP_transhyd_a. IPR004003. NADP_transhyd_b. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01262. AlaDh_PNT_C. 1 hit. PF05222. AlaDh_PNT_N. 1 hit. PF02233. PNTB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00561. pntA. 1 hit.
PROSITE	PS00836. ALADH_PNT_1. 1 hit. PS00837. ALADH_PNT_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

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Entry information

Entry name

NNTM_MOUSE

Accession

Primary (citable) accession number: Q61941
Secondary accession number(s): Q9JK70

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	August 16, 2004
Last modified:	October 13, 2009
This is version 78 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents