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Protein

Protein CBFA2T1

Gene

Runx1t1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Transcriptional corepressor which facilitates transcriptional repression via its association with DNA-binding transcription factors and recruitment of other corepressors and histone-modifying enzymes. Can repress the expression of MMP7 in a ZBTB33-dependent manner. Can repress transactivation mediated by TCF12 (By similarity). Acts as a negative regulator of adipogenesis (PubMed:23527555).By similarity1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri488 – 524MYND-typePROSITE-ProRule annotationAdd BLAST37

GO - Molecular functioni

GO - Biological processi

  • fat cell differentiation Source: MGI
  • negative regulation of fat cell differentiation Source: UniProtKB
  • negative regulation of neuron projection development Source: UniProtKB
  • negative regulation of transcription, DNA-templated Source: UniProtKB
  • regulation of DNA binding Source: MGI
  • regulation of transcription, DNA-templated Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Protein CBFA2T1
Alternative name(s):
Protein MTG8
Gene namesi
Name:Runx1t1
Synonyms:Cbfa2t1, Cbfa2t1h, Mtg8
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:104793. Runx1t1.

Subcellular locationi

  • Nucleus PROSITE-ProRule annotation

  • Note: Colocalizes with ATN1 in discrete nuclear dots.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002183001 – 577Protein CBFA2T1Add BLAST577

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei14PhosphoserineBy similarity1
Modified residuei390PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ61909.
PaxDbiQ61909.
PRIDEiQ61909.

PTM databases

iPTMnetiQ61909.
PhosphoSitePlusiQ61909.

Expressioni

Gene expression databases

BgeeiENSMUSG00000006586.
ExpressionAtlasiQ61909. baseline and differential.
GenevisibleiQ61909. MM.

Interactioni

Subunit structurei

Homotetramer (By similarity). Heterotetramer with CBFA2T2 and CBFA2T3 (By similarity). Interacts with TCF12, SIN3A, HDAC1, HDAC2, HDAC3, NCOR1 and NCOR2. Interacts with ATN1 (via its N-terminus); the interaction enhances the transcriptional repression (By similarity).By similarity

GO - Molecular functioni

  • protein homodimerization activity Source: MGI

Protein-protein interaction databases

BioGridi198520. 7 interactors.
IntActiQ61909. 2 interactors.
STRINGi10090.ENSMUSP00000095857.

Structurei

3D structure databases

ProteinModelPortaliQ61909.
SMRiQ61909.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini93 – 188TAFHPROSITE-ProRule annotationAdd BLAST96

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni310 – 356Important for oligomerizationBy similarityAdd BLAST47
Regioni310 – 356Nervy homology region 2 (NHR2)By similarityAdd BLAST47
Regioni416 – 465Nervy homology region 3 (NHR3)By similarityAdd BLAST50

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi30 – 33Poly-Pro4
Compositional biasi75 – 81Poly-Ser7
Compositional biasi263 – 270Poly-Pro8
Compositional biasi380 – 386Poly-Ser7

Domaini

The TAFH domain mediates interaction with transcription regulators.By similarity
Nervy homology region 2 (NHR2) mediates homo- and possibly heterotypic oligomerization by forming a four-helix bundle tetrameric structure.By similarity

Sequence similaritiesi

Belongs to the CBFA2T family.Curated
Contains 1 MYND-type zinc finger.PROSITE-ProRule annotation
Contains 1 TAFH (NHR1) domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri488 – 524MYND-typePROSITE-ProRule annotationAdd BLAST37

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiENOG410IG1Z. Eukaryota.
ENOG410XR30. LUCA.
GeneTreeiENSGT00390000013479.
HOGENOMiHOG000049245.
HOVERGENiHBG000169.
InParanoidiQ61909.
KOiK10053.

Family and domain databases

InterProiIPR013290. CBFA2T1.
IPR013289. CBFA2T1/2/3.
IPR014896. NHR2.
IPR003894. TAFH_NHR1.
IPR002893. Znf_MYND.
[Graphical view]
PANTHERiPTHR10379:SF5. PTHR10379:SF5. 1 hit.
PfamiPF08788. NHR2. 1 hit.
PF07531. TAFH. 1 hit.
PF01753. zf-MYND. 1 hit.
[Graphical view]
PRINTSiPR01875. ETOFAMILY.
PR01876. MTG8PROTEIN.
SMARTiSM00549. TAFH. 1 hit.
[Graphical view]
PROSITEiPS51119. TAFH. 1 hit.
PS01360. ZF_MYND_1. 1 hit.
PS50865. ZF_MYND_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q61909-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPDRTEKHST MPDSPVDVKT QSRLTPPAMP PPPTTQGAPR TSSFTPTTLT
60 70 80 90 100
NGTSHSPTAL NGAPSPPNGF SNGPSSSSSS SLANQQLPPA CGARQLSKLK
110 120 130 140 150
RFLTTLQQFG NDISPEIGER VRTLVLGLVN STLTIEEFHS KLQEATNFPL
160 170 180 190 200
RPFVIPFLKA NLPLLQRELL HCARLAKQNP AQYLAQHEQL LLDASTTSPV
210 220 230 240 250
DSSELLLDVN ENGKRRTPDR TKENGFDREP LHSEHPSKRP CTISPGQRYS
260 270 280 290 300
PNNGLSYQPN GLPHPTPPPP QHYRLDDMAI AHHYRDSYRH PSHRDLRDRN
310 320 330 340 350
RPMGLHGTRQ EEMIDHRLTD REWAEEWKHL DHLLNCIMDM VEKTRRSLTV
360 370 380 390 400
LRRCQEADRE ELNYWIRRYS DAEDLKKGGS SSSSHSRQQS PVNPDPVALD
410 420 430 440 450
AHREFLHRPA SGYVPEEIWK KAEEAVNEVK RQAMTELQKA VSEAERKAHD
460 470 480 490 500
MITTERAKME RTVAEAKRQA AEDALAVINQ QEDSSESCWN CGRKASETCS
510 520 530 540 550
GCNTARYCGS FCQHKDWEKH HHICGQTLQA PQQGDTPAVS SSVTPSSGAG
560 570
SPMDTPPAAT PRSTTPGTPS TIETTPR
Length:577
Mass (Da):64,338
Last modified:November 1, 1996 - v1
Checksum:i2656F185318C4B11
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D32007 mRNA. Translation: BAA06774.1.
CCDSiCCDS17978.1.
RefSeqiNP_033952.1. NM_009822.3.
UniGeneiMm.470961.
Mm.4909.

Genome annotation databases

EnsembliENSMUST00000098256; ENSMUSP00000095856; ENSMUSG00000006586.
GeneIDi12395.
KEGGimmu:12395.
UCSCiuc008sax.3. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D32007 mRNA. Translation: BAA06774.1.
CCDSiCCDS17978.1.
RefSeqiNP_033952.1. NM_009822.3.
UniGeneiMm.470961.
Mm.4909.

3D structure databases

ProteinModelPortaliQ61909.
SMRiQ61909.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198520. 7 interactors.
IntActiQ61909. 2 interactors.
STRINGi10090.ENSMUSP00000095857.

PTM databases

iPTMnetiQ61909.
PhosphoSitePlusiQ61909.

Proteomic databases

MaxQBiQ61909.
PaxDbiQ61909.
PRIDEiQ61909.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000098256; ENSMUSP00000095856; ENSMUSG00000006586.
GeneIDi12395.
KEGGimmu:12395.
UCSCiuc008sax.3. mouse.

Organism-specific databases

CTDi862.
MGIiMGI:104793. Runx1t1.

Phylogenomic databases

eggNOGiENOG410IG1Z. Eukaryota.
ENOG410XR30. LUCA.
GeneTreeiENSGT00390000013479.
HOGENOMiHOG000049245.
HOVERGENiHBG000169.
InParanoidiQ61909.
KOiK10053.

Miscellaneous databases

PROiQ61909.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000006586.
ExpressionAtlasiQ61909. baseline and differential.
GenevisibleiQ61909. MM.

Family and domain databases

InterProiIPR013290. CBFA2T1.
IPR013289. CBFA2T1/2/3.
IPR014896. NHR2.
IPR003894. TAFH_NHR1.
IPR002893. Znf_MYND.
[Graphical view]
PANTHERiPTHR10379:SF5. PTHR10379:SF5. 1 hit.
PfamiPF08788. NHR2. 1 hit.
PF07531. TAFH. 1 hit.
PF01753. zf-MYND. 1 hit.
[Graphical view]
PRINTSiPR01875. ETOFAMILY.
PR01876. MTG8PROTEIN.
SMARTiSM00549. TAFH. 1 hit.
[Graphical view]
PROSITEiPS51119. TAFH. 1 hit.
PS01360. ZF_MYND_1. 1 hit.
PS50865. ZF_MYND_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMTG8_MOUSE
AccessioniPrimary (citable) accession number: Q61909
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.