Reviewed, UniProtKB/Swiss-Prot Q61907 (PEMT_MOUSE)

Names and origin

Protein names

Recommended name:
    Phosphatidylethanolamine N-methyltransferase
      Short name=PEAMT
      Short name=PEMT
    EC=2.1.1.17
Alternative name(s):
    PEMT2

Gene names

Name:	Pemt
Synonyms:	Pempt, Pemt2

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	199 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes three sequential methylation of phosphatidylethanolamine (PE) by AdoMet, thus producing phosphatidylcholine (PC).
Catalytic activity	S-adenosyl-L-methionine + phosphatidylethanolamine = S-adenosyl-L-homocysteine + phosphatidyl-N-methylethanolamine.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein Potential. Mitochondrion membrane; Multi-pass membrane protein Potential.
Tissue specificity	Liver.
Sequence similarities	Belongs to the PEMT/PEM2 methyltransferase family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Endoplasmic reticulum Membrane Mitochondrion
Domain	Transmembrane
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from direct assay. Source: MGI integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	phosphatidylethanolamine N-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 199	198	Phosphatidylethanolamine N-methyltransferase		PRO_0000193921
Regions
	Transmembrane	13 – 33	21	Potential
	Transmembrane	46 – 66	21	Potential
	Transmembrane	91 – 111	21	Potential
	Transmembrane	158 – 178	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q61907-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: E0DD37BD5C483DDD	FASTA	199	22,516
	10 20 30 40 50 60 MSWLLGYMDP TEPSFVAAVI TIVFNPLFWN VVARWEQRTR KLSRAFGSPH LACYSLGICI 70 80 90 100 110 120 LLLNILRSHC FTQAMMSQPK MEGLDNHTTY FLGLAFLGWG FVFVLSSFYA LGFTGTFLGD 130 140 150 160 170 180 YFGILKESRV TTFPFSVLDN PMYWGSTANY LGWALMHASP TGLLLTVVVA IVNVVALLYE 190 EPFTAEIYRQ KATRLHKRS

References

[1]	"Characterization of the murine phosphatidylethanolamine N-methyltransferase-2 gene." Walkey C.J., Cui Z., Agellon L.B., Vance D.E. J. Lipid Res. 37:2341-2350(1996) [PubMed: 8978486] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129/J. Tissue: Liver.
[2]	"Disruption of the murine gene encoding phosphatidylethanolamine N-methyltransferase." Walkey C.J., Donohue L.R., Bronson R., Agellon L.B., Vance D.E. Proc. Natl. Acad. Sci. U.S.A. 94:12880-12885(1997) [PubMed: 9371769] [Abstract] Cited for: CHARACTERIZATION.
[3]	"Biochemical and evolutionary significance of phospholipid methylation." Walkey C.J., Yu L., Agellon L.B., Vance D.E. J. Biol. Chem. 273:27043-27046(1998) [PubMed: 9765216] [Abstract] Cited for: CHARACTERIZATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U25051 , U25046, U25047, U25048, U25049, U25050 Genomic DNA. Translation: AAA67686.1.
IPI	IPI00380362.
UniGene	Mm.2731
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSMUSG00000000301. Mus musculus. [Contig view]
Organism-specific databases
MGI	MGI:104535. Pemt.
Phylogenomic databases
HOVERGEN	Q61907.
Enzyme and pathway databases
BRENDA	2.1.1.17. 244.
Gene expression databases
ArrayExpress	Q61907.
Bgee	Q61907.
CleanEx	MM_PEMT.
GermOnline	ENSMUSG00000000301. Mus musculus.
Family and domain databases
InterPro	IPR007318. PEMT. [Graphical view]
PANTHER	PTHR15458. PEMT. 1 hit.
Pfam	PF04191. PEMT. 1 hit. [Graphical view]
PIRSF	PIRSF005444. PEMT. 1 hit.
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

PEMT_MOUSE

Accession

Primary (citable) accession number: Q61907

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	January 23, 2007
Last modified:	February 10, 2009
This is version 58 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)