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Q61881 (MCM7_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 124. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA replication licensing factor MCM7

EC=3.6.4.12
Alternative name(s):
CDC47 homolog
Gene names
Name:Mcm7
Synonyms:Cdc47, Mcmd7
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length719 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage.

Catalytic activity

ATP + H2O = ADP + phosphate.

Subunit structure

Component of the MCM2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order MCM2-MCM6-MCM4-MCM7-MCM3-MCM5. Interacts with the ATR-ATRIP complex and with RAD17. Interacts with TIPIN. Interacts with MCMBP By similarity.

Subcellular location

Nucleus By similarity.

Post-translational modification

O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner By similarity.

Sequence similarities

Belongs to the MCM family.

Contains 1 MCM domain.

Ontologies

Keywords
   Biological processCell cycle
DNA replication
   Cellular componentNucleus
   LigandATP-binding
DNA-binding
Nucleotide-binding
   Molecular functionHelicase
Hydrolase
   PTMAcetylation
Glycoprotein
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processDNA replication initiation

Inferred from electronic annotation. Source: InterPro

DNA unwinding involved in DNA replication

Inferred from physical interaction PubMed 15917436. Source: MGI

cell cycle

Inferred from electronic annotation. Source: UniProtKB-KW

cell proliferation

Inferred from direct assay PubMed 12972605. Source: MGI

cellular response to DNA damage stimulus

Inferred from electronic annotation. Source: Ensembl

cellular response to epidermal growth factor stimulus

Inferred from electronic annotation. Source: Ensembl

regulation of phosphorylation

Inferred from electronic annotation. Source: Ensembl

response to drug

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentMCM complex

Inferred from sequence or structural similarity. Source: UniProtKB

cytosol

Inferred from electronic annotation. Source: Ensembl

nucleus

Inferred from direct assay PubMed 12972605. Source: MGI

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

ATP-dependent DNA helicase activity

Inferred from electronic annotation. Source: Ensembl

protein binding

Inferred from physical interaction PubMed 16289477. Source: IntAct

single-stranded DNA binding

Inferred from physical interaction PubMed 15917436. Source: MGI

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Cdkn1bP464142EBI-457180,EBI-1005742

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 719718DNA replication licensing factor MCM7
PRO_0000194120

Regions

Domain332 – 538207MCM
Nucleotide binding381 – 3888ATP Potential
Region521 – 56444Interaction with RAD17 By similarity
Region577 – 719143Interaction with ATRIP By similarity
Motif513 – 5164Arginine finger

Amino acid modifications

Modified residue21N-acetylalanine By similarity
Modified residue3651Phosphoserine By similarity
Modified residue5001Phosphoserine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q61881 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: 019AE8E04BB8EB3C

FASTA71981,211
        10         20         30         40         50         60 
MALKDYAIEK EKVKKFLQEF YYENELGKKQ FKYGTQLVHL AHREQVALYV DLDDIAEDDP 

        70         80         90        100        110        120 
ELVDSICENA KRYSRLFGDV VQELLPEYKE KEVVNKDVLD VYIEHRLMME QRSRDPGAVR 

       130        140        150        160        170        180 
NPQNQYPSEL MRRFELYFRG PSSSKPRVIR EVRADSVGKL LTVRGIVTRV SEVKPRMVVA 

       190        200        210        220        230        240 
TYTCDQCGAE TYQPIQSPTF MPLIMCPSQE CQTNRSGGRL YLQTRGSKFV KFQEMKIQEH 

       250        260        270        280        290        300 
SDQVPVGNIP RSITVVLEGE NTRIAQPGDH VSVTGIFLPV LRTGFQQMAQ GLLSETYLEA 

       310        320        330        340        350        360 
HWIVKMTKSD DDVSGAGELS SEELKQIAEE DFYEKLAASI APEIYGHEDV KKALLLLLVG 

       370        380        390        400        410        420 
GVDQSPQGMK IRGNIHICLM GDPGVAKSQL LSYIDRLAPR SQYTTGRGSS GVGLTAAVLR 

       430        440        450        460        470        480 
DSVSGELTLE GGALVLADQG VCCIDEFDKM AEADRTAIHE VMEQQTISIA KAGILTTLNA 

       490        500        510        520        530        540 
RCSILAAANP AYGRYNPRRS LEQNVQLPAA LLSRFDLLWL IQDRPDRDND LRLAQHITYV 

       550        560        570        580        590        600 
HQHSRQPPAQ FEPLDMKLMR RYIAMCHERQ PTVPESLADY ITAAYVEMRR EARASKDATY 

       610        620        630        640        650        660 
TSARTLLAIL RLSTALARLR MVDIVEKEDV NEAIRLMEMS KDSLLGEKGQ TARTQRPADV 

       670        680        690        700        710 
IFATIRELVS RGRSVHFSEA EQRCISRGFT PAQFQAALDE YEELNVWQVN TSRTRITFV 

« Hide

References

« Hide 'large scale' references
[1]"Sequence of mouse CDC47, a member of the minichromosome maintenance (Mcm) family involved in the DNA replication licensing system."
Takizawa N., Kimura H., Sugimoto K.
Gene 167:343-344(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: BALB/c.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D26091 mRNA. Translation: BAA05084.1.
BC065164 mRNA. Translation: AAH65164.1.
BC066024 mRNA. Translation: AAH66024.1.
CCDSCCDS19793.1.
PIRJC4580.
RefSeqNP_032594.1. NM_008568.2.
UniGeneMm.378965.

3D structure databases

ProteinModelPortalQ61881.
SMRQ61881. Positions 81-280.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid201350. 3 interactions.
DIPDIP-45877N.
IntActQ61881. 2 interactions.
MINTMINT-1780090.

PTM databases

PhosphoSiteQ61881.

Proteomic databases

MaxQBQ61881.
PaxDbQ61881.
PRIDEQ61881.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000000505; ENSMUSP00000000505; ENSMUSG00000029730.
GeneID17220.
KEGGmmu:17220.
UCSCuc009aeu.1. mouse.

Organism-specific databases

CTD4176.
MGIMGI:1298398. Mcm7.

Phylogenomic databases

eggNOGCOG1241.
GeneTreeENSGT00670000098113.
HOVERGENHBG000741.
InParanoidQ61881.
KOK02210.
OMADINICLM.
OrthoDBEOG7N0C42.
TreeFamTF300400.

Gene expression databases

ArrayExpressQ61881.
BgeeQ61881.
GenevestigatorQ61881.

Family and domain databases

Gene3D2.40.50.140. 2 hits.
3.40.50.300. 1 hit.
InterProIPR003593. AAA+_ATPase.
IPR008050. MCM7.
IPR018525. MCM_CS.
IPR001208. MCM_DNA-dep_ATPase.
IPR027925. MCM_N.
IPR012340. NA-bd_OB-fold.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERPTHR11630:SF26. PTHR11630:SF26. 1 hit.
PfamPF00493. MCM. 1 hit.
PF14551. MCM_N. 1 hit.
[Graphical view]
PRINTSPR01657. MCMFAMILY.
PR01663. MCMPROTEIN7.
SMARTSM00382. AAA. 1 hit.
SM00350. MCM. 1 hit.
[Graphical view]
SUPFAMSSF50249. SSF50249. 2 hits.
SSF52540. SSF52540. 1 hit.
PROSITEPS00847. MCM_1. 1 hit.
PS50051. MCM_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio291622.
PROQ61881.
SOURCESearch...

Entry information

Entry nameMCM7_MOUSE
AccessionPrimary (citable) accession number: Q61881
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: July 9, 2014
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot