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Protein

DNA replication licensing factor MCM7

Gene

Mcm7

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage.

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi381 – 3888ATPSequence Analysis

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ATP-dependent DNA helicase activity Source: Ensembl
  3. single-stranded DNA binding Source: MGI

GO - Biological processi

  1. cell cycle Source: UniProtKB-KW
  2. cell proliferation Source: MGI
  3. cellular response to DNA damage stimulus Source: MGI
  4. cellular response to epidermal growth factor stimulus Source: Ensembl
  5. DNA replication initiation Source: InterPro
  6. DNA unwinding involved in DNA replication Source: MGI
  7. regulation of phosphorylation Source: MGI
  8. response to drug Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

Cell cycle, DNA replication

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_217374. Unwinding of DNA.
REACT_230841. Assembly of the pre-replicative complex.
REACT_232236. Activation of ATR in response to replication stress.
REACT_236939. Removal of licensing factors from origins.
REACT_245230. Orc1 removal from chromatin.
REACT_254983. Activation of the pre-replicative complex.
REACT_262530. Switching of origins to a post-replicative state.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA replication licensing factor MCM7 (EC:3.6.4.12)
Alternative name(s):
CDC47 homolog
Gene namesi
Name:Mcm7
Synonyms:Cdc47, Mcmd7
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 5

Organism-specific databases

MGIiMGI:1298398. Mcm7.

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. cytoplasm Source: MGI
  2. cytosol Source: Ensembl
  3. MCM complex Source: UniProtKB
  4. membrane Source: MGI
  5. nucleoplasm Source: MGI
  6. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 719718DNA replication licensing factor MCM7PRO_0000194120Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei365 – 3651PhosphoserineBy similarity
Modified residuei500 – 5001PhosphoserineBy similarity

Post-translational modificationi

O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.By similarity

Keywords - PTMi

Acetylation, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ61881.
PaxDbiQ61881.
PRIDEiQ61881.

PTM databases

PhosphoSiteiQ61881.

Expressioni

Gene expression databases

BgeeiQ61881.
ExpressionAtlasiQ61881. baseline and differential.
GenevestigatoriQ61881.

Interactioni

Subunit structurei

Component of the MCM2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order MCM2-MCM6-MCM4-MCM7-MCM3-MCM5. Interacts with the ATR-ATRIP complex and with RAD17. Interacts with TIPIN. Interacts with MCMBP (By similarity). Interacts with ANKRD17.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Cdkn1bP464142EBI-457180,EBI-1005742

Protein-protein interaction databases

BioGridi201350. 3 interactions.
DIPiDIP-45877N.
IntActiQ61881. 2 interactions.
MINTiMINT-1780090.

Structurei

3D structure databases

ProteinModelPortaliQ61881.
SMRiQ61881. Positions 11-281.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini332 – 538207MCMAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni521 – 56444Interaction with RAD17By similarityAdd
BLAST
Regioni577 – 719143Interaction with ATRIPBy similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi513 – 5164Arginine finger

Sequence similaritiesi

Belongs to the MCM family.Curated
Contains 1 MCM domain.Curated

Phylogenomic databases

eggNOGiCOG1241.
GeneTreeiENSGT00670000098113.
HOVERGENiHBG000741.
InParanoidiQ61881.
KOiK02210.
OMAiPTPYTGF.
OrthoDBiEOG7N0C42.
TreeFamiTF300400.

Family and domain databases

Gene3Di2.40.50.140. 2 hits.
3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR008050. MCM7.
IPR018525. MCM_CS.
IPR001208. MCM_DNA-dep_ATPase.
IPR027925. MCM_N.
IPR012340. NA-bd_OB-fold.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR11630:SF26. PTHR11630:SF26. 1 hit.
PfamiPF00493. MCM. 1 hit.
PF14551. MCM_N. 1 hit.
[Graphical view]
PRINTSiPR01657. MCMFAMILY.
PR01663. MCMPROTEIN7.
SMARTiSM00382. AAA. 1 hit.
SM00350. MCM. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 2 hits.
SSF52540. SSF52540. 1 hit.
PROSITEiPS00847. MCM_1. 1 hit.
PS50051. MCM_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q61881-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALKDYAIEK EKVKKFLQEF YYENELGKKQ FKYGTQLVHL AHREQVALYV
60 70 80 90 100
DLDDIAEDDP ELVDSICENA KRYSRLFGDV VQELLPEYKE KEVVNKDVLD
110 120 130 140 150
VYIEHRLMME QRSRDPGAVR NPQNQYPSEL MRRFELYFRG PSSSKPRVIR
160 170 180 190 200
EVRADSVGKL LTVRGIVTRV SEVKPRMVVA TYTCDQCGAE TYQPIQSPTF
210 220 230 240 250
MPLIMCPSQE CQTNRSGGRL YLQTRGSKFV KFQEMKIQEH SDQVPVGNIP
260 270 280 290 300
RSITVVLEGE NTRIAQPGDH VSVTGIFLPV LRTGFQQMAQ GLLSETYLEA
310 320 330 340 350
HWIVKMTKSD DDVSGAGELS SEELKQIAEE DFYEKLAASI APEIYGHEDV
360 370 380 390 400
KKALLLLLVG GVDQSPQGMK IRGNIHICLM GDPGVAKSQL LSYIDRLAPR
410 420 430 440 450
SQYTTGRGSS GVGLTAAVLR DSVSGELTLE GGALVLADQG VCCIDEFDKM
460 470 480 490 500
AEADRTAIHE VMEQQTISIA KAGILTTLNA RCSILAAANP AYGRYNPRRS
510 520 530 540 550
LEQNVQLPAA LLSRFDLLWL IQDRPDRDND LRLAQHITYV HQHSRQPPAQ
560 570 580 590 600
FEPLDMKLMR RYIAMCHERQ PTVPESLADY ITAAYVEMRR EARASKDATY
610 620 630 640 650
TSARTLLAIL RLSTALARLR MVDIVEKEDV NEAIRLMEMS KDSLLGEKGQ
660 670 680 690 700
TARTQRPADV IFATIRELVS RGRSVHFSEA EQRCISRGFT PAQFQAALDE
710
YEELNVWQVN TSRTRITFV
Length:719
Mass (Da):81,211
Last modified:November 1, 1997 - v1
Checksum:i019AE8E04BB8EB3C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D26091 mRNA. Translation: BAA05084.1.
BC065164 mRNA. Translation: AAH65164.1.
BC066024 mRNA. Translation: AAH66024.1.
CCDSiCCDS19793.1.
PIRiJC4580.
RefSeqiNP_032594.1. NM_008568.2.
UniGeneiMm.378965.

Genome annotation databases

EnsembliENSMUST00000000505; ENSMUSP00000000505; ENSMUSG00000029730.
GeneIDi17220.
KEGGimmu:17220.
UCSCiuc009aeu.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D26091 mRNA. Translation: BAA05084.1.
BC065164 mRNA. Translation: AAH65164.1.
BC066024 mRNA. Translation: AAH66024.1.
CCDSiCCDS19793.1.
PIRiJC4580.
RefSeqiNP_032594.1. NM_008568.2.
UniGeneiMm.378965.

3D structure databases

ProteinModelPortaliQ61881.
SMRiQ61881. Positions 11-281.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201350. 3 interactions.
DIPiDIP-45877N.
IntActiQ61881. 2 interactions.
MINTiMINT-1780090.

PTM databases

PhosphoSiteiQ61881.

Proteomic databases

MaxQBiQ61881.
PaxDbiQ61881.
PRIDEiQ61881.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000000505; ENSMUSP00000000505; ENSMUSG00000029730.
GeneIDi17220.
KEGGimmu:17220.
UCSCiuc009aeu.1. mouse.

Organism-specific databases

CTDi4176.
MGIiMGI:1298398. Mcm7.

Phylogenomic databases

eggNOGiCOG1241.
GeneTreeiENSGT00670000098113.
HOVERGENiHBG000741.
InParanoidiQ61881.
KOiK02210.
OMAiPTPYTGF.
OrthoDBiEOG7N0C42.
TreeFamiTF300400.

Enzyme and pathway databases

ReactomeiREACT_217374. Unwinding of DNA.
REACT_230841. Assembly of the pre-replicative complex.
REACT_232236. Activation of ATR in response to replication stress.
REACT_236939. Removal of licensing factors from origins.
REACT_245230. Orc1 removal from chromatin.
REACT_254983. Activation of the pre-replicative complex.
REACT_262530. Switching of origins to a post-replicative state.

Miscellaneous databases

NextBioi291622.
PROiQ61881.
SOURCEiSearch...

Gene expression databases

BgeeiQ61881.
ExpressionAtlasiQ61881. baseline and differential.
GenevestigatoriQ61881.

Family and domain databases

Gene3Di2.40.50.140. 2 hits.
3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR008050. MCM7.
IPR018525. MCM_CS.
IPR001208. MCM_DNA-dep_ATPase.
IPR027925. MCM_N.
IPR012340. NA-bd_OB-fold.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR11630:SF26. PTHR11630:SF26. 1 hit.
PfamiPF00493. MCM. 1 hit.
PF14551. MCM_N. 1 hit.
[Graphical view]
PRINTSiPR01657. MCMFAMILY.
PR01663. MCMPROTEIN7.
SMARTiSM00382. AAA. 1 hit.
SM00350. MCM. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 2 hits.
SSF52540. SSF52540. 1 hit.
PROSITEiPS00847. MCM_1. 1 hit.
PS50051. MCM_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence of mouse CDC47, a member of the minichromosome maintenance (Mcm) family involved in the DNA replication licensing system."
    Takizawa N., Kimura H., Sugimoto K.
    Gene 167:343-344(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain.

Entry informationi

Entry nameiMCM7_MOUSE
AccessioniPrimary (citable) accession number: Q61881
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: March 4, 2015
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.