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Q61881

- MCM7_MOUSE

UniProt

Q61881 - MCM7_MOUSE

Protein

DNA replication licensing factor MCM7

Gene

Mcm7

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 126 (01 Oct 2014)
      Sequence version 1 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage.

    Catalytic activityi

    ATP + H2O = ADP + phosphate.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi381 – 3888ATPSequence Analysis

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. ATP-dependent DNA helicase activity Source: Ensembl
    3. protein binding Source: IntAct
    4. single-stranded DNA binding Source: MGI

    GO - Biological processi

    1. cell cycle Source: UniProtKB-KW
    2. cell proliferation Source: MGI
    3. cellular response to DNA damage stimulus Source: Ensembl
    4. cellular response to epidermal growth factor stimulus Source: Ensembl
    5. DNA replication initiation Source: InterPro
    6. DNA unwinding involved in DNA replication Source: MGI
    7. regulation of phosphorylation Source: Ensembl
    8. response to drug Source: Ensembl

    Keywords - Molecular functioni

    Helicase, Hydrolase

    Keywords - Biological processi

    Cell cycle, DNA replication

    Keywords - Ligandi

    ATP-binding, DNA-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_217374. Unwinding of DNA.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA replication licensing factor MCM7 (EC:3.6.4.12)
    Alternative name(s):
    CDC47 homolog
    Gene namesi
    Name:Mcm7
    Synonyms:Cdc47, Mcmd7
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 5

    Organism-specific databases

    MGIiMGI:1298398. Mcm7.

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. cytosol Source: Ensembl
    2. MCM complex Source: UniProtKB
    3. nucleus Source: MGI

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 719718DNA replication licensing factor MCM7PRO_0000194120Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity
    Modified residuei365 – 3651PhosphoserineBy similarity
    Modified residuei500 – 5001PhosphoserineBy similarity

    Post-translational modificationi

    O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.By similarity

    Keywords - PTMi

    Acetylation, Glycoprotein, Phosphoprotein

    Proteomic databases

    MaxQBiQ61881.
    PaxDbiQ61881.
    PRIDEiQ61881.

    PTM databases

    PhosphoSiteiQ61881.

    Expressioni

    Gene expression databases

    ArrayExpressiQ61881.
    BgeeiQ61881.
    GenevestigatoriQ61881.

    Interactioni

    Subunit structurei

    Component of the MCM2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order MCM2-MCM6-MCM4-MCM7-MCM3-MCM5. Interacts with the ATR-ATRIP complex and with RAD17. Interacts with TIPIN. Interacts with MCMBP By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Cdkn1bP464142EBI-457180,EBI-1005742

    Protein-protein interaction databases

    BioGridi201350. 3 interactions.
    DIPiDIP-45877N.
    IntActiQ61881. 2 interactions.
    MINTiMINT-1780090.

    Structurei

    3D structure databases

    ProteinModelPortaliQ61881.
    SMRiQ61881. Positions 81-280.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini332 – 538207MCMAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni521 – 56444Interaction with RAD17By similarityAdd
    BLAST
    Regioni577 – 719143Interaction with ATRIPBy similarityAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi513 – 5164Arginine finger

    Sequence similaritiesi

    Belongs to the MCM family.Curated
    Contains 1 MCM domain.Curated

    Phylogenomic databases

    eggNOGiCOG1241.
    GeneTreeiENSGT00670000098113.
    HOVERGENiHBG000741.
    InParanoidiQ61881.
    KOiK02210.
    OMAiDINICLM.
    OrthoDBiEOG7N0C42.
    TreeFamiTF300400.

    Family and domain databases

    Gene3Di2.40.50.140. 2 hits.
    3.40.50.300. 1 hit.
    InterProiIPR003593. AAA+_ATPase.
    IPR008050. MCM7.
    IPR018525. MCM_CS.
    IPR001208. MCM_DNA-dep_ATPase.
    IPR027925. MCM_N.
    IPR012340. NA-bd_OB-fold.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PANTHERiPTHR11630:SF26. PTHR11630:SF26. 1 hit.
    PfamiPF00493. MCM. 1 hit.
    PF14551. MCM_N. 1 hit.
    [Graphical view]
    PRINTSiPR01657. MCMFAMILY.
    PR01663. MCMPROTEIN7.
    SMARTiSM00382. AAA. 1 hit.
    SM00350. MCM. 1 hit.
    [Graphical view]
    SUPFAMiSSF50249. SSF50249. 2 hits.
    SSF52540. SSF52540. 1 hit.
    PROSITEiPS00847. MCM_1. 1 hit.
    PS50051. MCM_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q61881-1 [UniParc]FASTAAdd to Basket

    « Hide

    MALKDYAIEK EKVKKFLQEF YYENELGKKQ FKYGTQLVHL AHREQVALYV    50
    DLDDIAEDDP ELVDSICENA KRYSRLFGDV VQELLPEYKE KEVVNKDVLD 100
    VYIEHRLMME QRSRDPGAVR NPQNQYPSEL MRRFELYFRG PSSSKPRVIR 150
    EVRADSVGKL LTVRGIVTRV SEVKPRMVVA TYTCDQCGAE TYQPIQSPTF 200
    MPLIMCPSQE CQTNRSGGRL YLQTRGSKFV KFQEMKIQEH SDQVPVGNIP 250
    RSITVVLEGE NTRIAQPGDH VSVTGIFLPV LRTGFQQMAQ GLLSETYLEA 300
    HWIVKMTKSD DDVSGAGELS SEELKQIAEE DFYEKLAASI APEIYGHEDV 350
    KKALLLLLVG GVDQSPQGMK IRGNIHICLM GDPGVAKSQL LSYIDRLAPR 400
    SQYTTGRGSS GVGLTAAVLR DSVSGELTLE GGALVLADQG VCCIDEFDKM 450
    AEADRTAIHE VMEQQTISIA KAGILTTLNA RCSILAAANP AYGRYNPRRS 500
    LEQNVQLPAA LLSRFDLLWL IQDRPDRDND LRLAQHITYV HQHSRQPPAQ 550
    FEPLDMKLMR RYIAMCHERQ PTVPESLADY ITAAYVEMRR EARASKDATY 600
    TSARTLLAIL RLSTALARLR MVDIVEKEDV NEAIRLMEMS KDSLLGEKGQ 650
    TARTQRPADV IFATIRELVS RGRSVHFSEA EQRCISRGFT PAQFQAALDE 700
    YEELNVWQVN TSRTRITFV 719
    Length:719
    Mass (Da):81,211
    Last modified:November 1, 1997 - v1
    Checksum:i019AE8E04BB8EB3C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D26091 mRNA. Translation: BAA05084.1.
    BC065164 mRNA. Translation: AAH65164.1.
    BC066024 mRNA. Translation: AAH66024.1.
    CCDSiCCDS19793.1.
    PIRiJC4580.
    RefSeqiNP_032594.1. NM_008568.2.
    UniGeneiMm.378965.

    Genome annotation databases

    EnsembliENSMUST00000000505; ENSMUSP00000000505; ENSMUSG00000029730.
    GeneIDi17220.
    KEGGimmu:17220.
    UCSCiuc009aeu.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D26091 mRNA. Translation: BAA05084.1 .
    BC065164 mRNA. Translation: AAH65164.1 .
    BC066024 mRNA. Translation: AAH66024.1 .
    CCDSi CCDS19793.1.
    PIRi JC4580.
    RefSeqi NP_032594.1. NM_008568.2.
    UniGenei Mm.378965.

    3D structure databases

    ProteinModelPortali Q61881.
    SMRi Q61881. Positions 81-280.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 201350. 3 interactions.
    DIPi DIP-45877N.
    IntActi Q61881. 2 interactions.
    MINTi MINT-1780090.

    PTM databases

    PhosphoSitei Q61881.

    Proteomic databases

    MaxQBi Q61881.
    PaxDbi Q61881.
    PRIDEi Q61881.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000000505 ; ENSMUSP00000000505 ; ENSMUSG00000029730 .
    GeneIDi 17220.
    KEGGi mmu:17220.
    UCSCi uc009aeu.1. mouse.

    Organism-specific databases

    CTDi 4176.
    MGIi MGI:1298398. Mcm7.

    Phylogenomic databases

    eggNOGi COG1241.
    GeneTreei ENSGT00670000098113.
    HOVERGENi HBG000741.
    InParanoidi Q61881.
    KOi K02210.
    OMAi DINICLM.
    OrthoDBi EOG7N0C42.
    TreeFami TF300400.

    Enzyme and pathway databases

    Reactomei REACT_217374. Unwinding of DNA.

    Miscellaneous databases

    NextBioi 291622.
    PROi Q61881.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q61881.
    Bgeei Q61881.
    Genevestigatori Q61881.

    Family and domain databases

    Gene3Di 2.40.50.140. 2 hits.
    3.40.50.300. 1 hit.
    InterProi IPR003593. AAA+_ATPase.
    IPR008050. MCM7.
    IPR018525. MCM_CS.
    IPR001208. MCM_DNA-dep_ATPase.
    IPR027925. MCM_N.
    IPR012340. NA-bd_OB-fold.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    PANTHERi PTHR11630:SF26. PTHR11630:SF26. 1 hit.
    Pfami PF00493. MCM. 1 hit.
    PF14551. MCM_N. 1 hit.
    [Graphical view ]
    PRINTSi PR01657. MCMFAMILY.
    PR01663. MCMPROTEIN7.
    SMARTi SM00382. AAA. 1 hit.
    SM00350. MCM. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50249. SSF50249. 2 hits.
    SSF52540. SSF52540. 1 hit.
    PROSITEi PS00847. MCM_1. 1 hit.
    PS50051. MCM_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence of mouse CDC47, a member of the minichromosome maintenance (Mcm) family involved in the DNA replication licensing system."
      Takizawa N., Kimura H., Sugimoto K.
      Gene 167:343-344(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: BALB/c.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Brain.

    Entry informationi

    Entry nameiMCM7_MOUSE
    AccessioniPrimary (citable) accession number: Q61881
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 126 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3