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Q61881

- MCM7_MOUSE

UniProt

Q61881 - MCM7_MOUSE

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Protein

DNA replication licensing factor MCM7

Gene
Mcm7, Cdc47, Mcmd7
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage.

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi381 – 3888ATP Reviewed prediction

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ATP-dependent DNA helicase activity Source: Ensembl
  3. protein binding Source: IntAct
  4. single-stranded DNA binding Source: MGI

GO - Biological processi

  1. cell cycle Source: UniProtKB-KW
  2. cell proliferation Source: MGI
  3. cellular response to DNA damage stimulus Source: Ensembl
  4. cellular response to epidermal growth factor stimulus Source: Ensembl
  5. DNA replication initiation Source: InterPro
  6. DNA unwinding involved in DNA replication Source: MGI
  7. regulation of phosphorylation Source: Ensembl
  8. response to drug Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

Cell cycle, DNA replication

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_217374. Unwinding of DNA.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA replication licensing factor MCM7 (EC:3.6.4.12)
Alternative name(s):
CDC47 homolog
Gene namesi
Name:Mcm7
Synonyms:Cdc47, Mcmd7
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 5

Organism-specific databases

MGIiMGI:1298398. Mcm7.

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. cytosol Source: Ensembl
  2. MCM complex Source: UniProtKB
  3. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 719718DNA replication licensing factor MCM7PRO_0000194120Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine By similarity
Modified residuei365 – 3651Phosphoserine By similarity
Modified residuei500 – 5001Phosphoserine By similarity

Post-translational modificationi

O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner By similarity.

Keywords - PTMi

Acetylation, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ61881.
PaxDbiQ61881.
PRIDEiQ61881.

PTM databases

PhosphoSiteiQ61881.

Expressioni

Gene expression databases

ArrayExpressiQ61881.
BgeeiQ61881.
GenevestigatoriQ61881.

Interactioni

Subunit structurei

Component of the MCM2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order MCM2-MCM6-MCM4-MCM7-MCM3-MCM5. Interacts with the ATR-ATRIP complex and with RAD17. Interacts with TIPIN. Interacts with MCMBP By similarity.

Binary interactionsi

WithEntry#Exp.IntActNotes
Cdkn1bP464142EBI-457180,EBI-1005742

Protein-protein interaction databases

BioGridi201350. 3 interactions.
DIPiDIP-45877N.
IntActiQ61881. 2 interactions.
MINTiMINT-1780090.

Structurei

3D structure databases

ProteinModelPortaliQ61881.
SMRiQ61881. Positions 81-280.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini332 – 538207MCMAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni521 – 56444Interaction with RAD17 By similarityAdd
BLAST
Regioni577 – 719143Interaction with ATRIP By similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi513 – 5164Arginine finger

Sequence similaritiesi

Belongs to the MCM family.
Contains 1 MCM domain.

Phylogenomic databases

eggNOGiCOG1241.
GeneTreeiENSGT00670000098113.
HOVERGENiHBG000741.
InParanoidiQ61881.
KOiK02210.
OMAiDINICLM.
OrthoDBiEOG7N0C42.
TreeFamiTF300400.

Family and domain databases

Gene3Di2.40.50.140. 2 hits.
3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR008050. MCM7.
IPR018525. MCM_CS.
IPR001208. MCM_DNA-dep_ATPase.
IPR027925. MCM_N.
IPR012340. NA-bd_OB-fold.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR11630:SF26. PTHR11630:SF26. 1 hit.
PfamiPF00493. MCM. 1 hit.
PF14551. MCM_N. 1 hit.
[Graphical view]
PRINTSiPR01657. MCMFAMILY.
PR01663. MCMPROTEIN7.
SMARTiSM00382. AAA. 1 hit.
SM00350. MCM. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 2 hits.
SSF52540. SSF52540. 1 hit.
PROSITEiPS00847. MCM_1. 1 hit.
PS50051. MCM_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q61881-1 [UniParc]FASTAAdd to Basket

« Hide

MALKDYAIEK EKVKKFLQEF YYENELGKKQ FKYGTQLVHL AHREQVALYV    50
DLDDIAEDDP ELVDSICENA KRYSRLFGDV VQELLPEYKE KEVVNKDVLD 100
VYIEHRLMME QRSRDPGAVR NPQNQYPSEL MRRFELYFRG PSSSKPRVIR 150
EVRADSVGKL LTVRGIVTRV SEVKPRMVVA TYTCDQCGAE TYQPIQSPTF 200
MPLIMCPSQE CQTNRSGGRL YLQTRGSKFV KFQEMKIQEH SDQVPVGNIP 250
RSITVVLEGE NTRIAQPGDH VSVTGIFLPV LRTGFQQMAQ GLLSETYLEA 300
HWIVKMTKSD DDVSGAGELS SEELKQIAEE DFYEKLAASI APEIYGHEDV 350
KKALLLLLVG GVDQSPQGMK IRGNIHICLM GDPGVAKSQL LSYIDRLAPR 400
SQYTTGRGSS GVGLTAAVLR DSVSGELTLE GGALVLADQG VCCIDEFDKM 450
AEADRTAIHE VMEQQTISIA KAGILTTLNA RCSILAAANP AYGRYNPRRS 500
LEQNVQLPAA LLSRFDLLWL IQDRPDRDND LRLAQHITYV HQHSRQPPAQ 550
FEPLDMKLMR RYIAMCHERQ PTVPESLADY ITAAYVEMRR EARASKDATY 600
TSARTLLAIL RLSTALARLR MVDIVEKEDV NEAIRLMEMS KDSLLGEKGQ 650
TARTQRPADV IFATIRELVS RGRSVHFSEA EQRCISRGFT PAQFQAALDE 700
YEELNVWQVN TSRTRITFV 719
Length:719
Mass (Da):81,211
Last modified:November 1, 1997 - v1
Checksum:i019AE8E04BB8EB3C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D26091 mRNA. Translation: BAA05084.1.
BC065164 mRNA. Translation: AAH65164.1.
BC066024 mRNA. Translation: AAH66024.1.
CCDSiCCDS19793.1.
PIRiJC4580.
RefSeqiNP_032594.1. NM_008568.2.
UniGeneiMm.378965.

Genome annotation databases

EnsembliENSMUST00000000505; ENSMUSP00000000505; ENSMUSG00000029730.
GeneIDi17220.
KEGGimmu:17220.
UCSCiuc009aeu.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D26091 mRNA. Translation: BAA05084.1 .
BC065164 mRNA. Translation: AAH65164.1 .
BC066024 mRNA. Translation: AAH66024.1 .
CCDSi CCDS19793.1.
PIRi JC4580.
RefSeqi NP_032594.1. NM_008568.2.
UniGenei Mm.378965.

3D structure databases

ProteinModelPortali Q61881.
SMRi Q61881. Positions 81-280.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 201350. 3 interactions.
DIPi DIP-45877N.
IntActi Q61881. 2 interactions.
MINTi MINT-1780090.

PTM databases

PhosphoSitei Q61881.

Proteomic databases

MaxQBi Q61881.
PaxDbi Q61881.
PRIDEi Q61881.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000000505 ; ENSMUSP00000000505 ; ENSMUSG00000029730 .
GeneIDi 17220.
KEGGi mmu:17220.
UCSCi uc009aeu.1. mouse.

Organism-specific databases

CTDi 4176.
MGIi MGI:1298398. Mcm7.

Phylogenomic databases

eggNOGi COG1241.
GeneTreei ENSGT00670000098113.
HOVERGENi HBG000741.
InParanoidi Q61881.
KOi K02210.
OMAi DINICLM.
OrthoDBi EOG7N0C42.
TreeFami TF300400.

Enzyme and pathway databases

Reactomei REACT_217374. Unwinding of DNA.

Miscellaneous databases

NextBioi 291622.
PROi Q61881.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q61881.
Bgeei Q61881.
Genevestigatori Q61881.

Family and domain databases

Gene3Di 2.40.50.140. 2 hits.
3.40.50.300. 1 hit.
InterProi IPR003593. AAA+_ATPase.
IPR008050. MCM7.
IPR018525. MCM_CS.
IPR001208. MCM_DNA-dep_ATPase.
IPR027925. MCM_N.
IPR012340. NA-bd_OB-fold.
IPR027417. P-loop_NTPase.
[Graphical view ]
PANTHERi PTHR11630:SF26. PTHR11630:SF26. 1 hit.
Pfami PF00493. MCM. 1 hit.
PF14551. MCM_N. 1 hit.
[Graphical view ]
PRINTSi PR01657. MCMFAMILY.
PR01663. MCMPROTEIN7.
SMARTi SM00382. AAA. 1 hit.
SM00350. MCM. 1 hit.
[Graphical view ]
SUPFAMi SSF50249. SSF50249. 2 hits.
SSF52540. SSF52540. 1 hit.
PROSITEi PS00847. MCM_1. 1 hit.
PS50051. MCM_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence of mouse CDC47, a member of the minichromosome maintenance (Mcm) family involved in the DNA replication licensing system."
    Takizawa N., Kimura H., Sugimoto K.
    Gene 167:343-344(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain.

Entry informationi

Entry nameiMCM7_MOUSE
AccessioniPrimary (citable) accession number: Q61881
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: September 3, 2014
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi