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Protein

Myosin-10

Gene

Myh10

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. During cell spreading, plays an important role in cytoskeleton reorganization, focal contacts formation (in the central part but not the margins of spreading cells), and lamellipodial extension; this function is mechanically antagonized by MYH9 (By similarity). Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi178 – 185ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

  • actin cytoskeleton organization Source: MGI
  • actin filament-based movement Source: MGI
  • actomyosin structure organization Source: MGI
  • adult heart development Source: MGI
  • aorta development Source: MGI
  • axon guidance Source: MGI
  • axonogenesis Source: MGI
  • brain development Source: MGI
  • cardiac myofibril assembly Source: MGI
  • cardiac septum development Source: MGI
  • cell adhesion Source: UniProtKB-KW
  • cell proliferation Source: MGI
  • cerebellar Purkinje cell layer development Source: MGI
  • coronary vasculature development Source: MGI
  • exocytosis Source: MGI
  • fourth ventricle development Source: MGI
  • heart development Source: MGI
  • in utero embryonic development Source: MGI
  • lateral ventricle development Source: MGI
  • mitotic cytokinesis Source: MGI
  • modification of postsynaptic actin cytoskeleton Source: Ensembl
  • myofibril assembly Source: MGI
  • neuromuscular process controlling balance Source: MGI
  • neuron migration Source: MGI
  • neuron projection development Source: MGI
  • nuclear migration Source: MGI
  • plasma membrane repair Source: MGI
  • regulation of cell shape Source: MGI
  • retina development in camera-type eye Source: MGI
  • substrate-dependent cell migration, cell extension Source: MGI
  • third ventricle development Source: MGI
  • ventricular cardiac muscle cell development Source: MGI

Keywordsi

Molecular functionActin-binding, Calmodulin-binding, Motor protein, Myosin
Biological processCell adhesion, Cell shape
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-5627123. RHO GTPases activate PAKs.

Names & Taxonomyi

Protein namesi
Recommended name:
Myosin-10
Alternative name(s):
Cellular myosin heavy chain, type B
Myosin heavy chain 10
Myosin heavy chain, non-muscle IIb
Non-muscle myosin heavy chain B
Short name:
NMMHC-B
Non-muscle myosin heavy chain IIb
Short name:
NMMHC II-b
Short name:
NMMHC-IIB
Gene namesi
Name:Myh10
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1930780. Myh10.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell projection

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001234221 – 1976Myosin-10Add BLAST1976

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei18Omega-N-methylarginineCombined sources1
Modified residuei442N6-acetyllysineBy similarity1
Modified residuei1145PhosphoserineBy similarity1
Modified residuei1241N6-acetyllysineCombined sources1
Modified residuei1301N6-acetyllysineCombined sources1
Modified residuei1645N6-acetyllysineBy similarity1
Modified residuei1930Omega-N-methylarginineCombined sources1
Modified residuei1935PhosphoserineBy similarity1
Modified residuei1937PhosphoserineBy similarity1
Modified residuei1938PhosphoserineBy similarity1
Modified residuei1939PhosphoserineCombined sources1
Modified residuei1940Omega-N-methylarginineCombined sources1
Modified residuei1952PhosphoserineCombined sources1
Modified residuei1956PhosphoserineCombined sources1
Modified residuei1960PhosphothreonineBy similarity1
Modified residuei1975PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated by ABL2.1 Publication

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

EPDiQ61879.
MaxQBiQ61879.
PaxDbiQ61879.
PRIDEiQ61879.

PTM databases

iPTMnetiQ61879.
PhosphoSitePlusiQ61879.
SwissPalmiQ61879.

Expressioni

Tissue specificityi

In newborn kidney, expressed in the mesenchyme and ureteric buds.1 Publication

Gene expression databases

BgeeiENSMUSG00000020900.
CleanExiMM_MYH10.
ExpressionAtlasiQ61879. baseline and differential.
GenevisibleiQ61879. MM.

Interactioni

Subunit structurei

Myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2). Interacts with PLEKHG6 (By similarity). Interacts with ECM29 (By similarity). Interacts with LARP6 (By similarity). Interacts with MCC (By similarity). Interacts with KIF26B (PubMed:20439720). Interacts with C9orf135 homolog (By similarity).By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Kif26bQ7TNC63EBI-400918,EBI-15852098

GO - Molecular functioni

Protein-protein interaction databases

BioGridi218774. 147 interactors.
DIPiDIP-31956N.
IntActiQ61879. 155 interactors.
MINTiMINT-4102674.
STRINGi10090.ENSMUSP00000099671.

Structurei

3D structure databases

ProteinModelPortaliQ61879.
SMRiQ61879.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini31 – 81Myosin N-terminal SH3-likePROSITE-ProRule annotationAdd BLAST51
Domaini85 – 783Myosin motorPROSITE-ProRule annotationAdd BLAST699
Domaini786 – 815IQPROSITE-ProRule annotationAdd BLAST30

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni661 – 683Actin-bindingPROSITE-ProRule annotationAdd BLAST23

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili845 – 1976Sequence analysisAdd BLAST1132

Domaini

The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.

Sequence similaritiesi

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0160. Eukaryota.
KOG0161. Eukaryota.
COG5022. LUCA.
GeneTreeiENSGT00900000140837.
HOGENOMiHOG000173958.
HOVERGENiHBG004704.
InParanoidiQ61879.
KOiK10352.
PhylomeDBiQ61879.
TreeFamiTF333601.

Family and domain databases

Gene3Di2.30.30.360. 1 hit.
InterProiView protein in InterPro
IPR000048. IQ_motif_EF-hand-BS.
IPR001609. Myosin_head_motor_dom.
IPR004009. Myosin_N.
IPR008989. Myosin_S1_N.
IPR002928. Myosin_tail.
IPR027417. P-loop_NTPase.
PfamiView protein in Pfam
PF00612. IQ. 1 hit.
PF00063. Myosin_head. 1 hit.
PF02736. Myosin_N. 1 hit.
PF01576. Myosin_tail_1. 1 hit.
PRINTSiPR00193. MYOSINHEAVY.
SMARTiView protein in SMART
SM00015. IQ. 1 hit.
SM00242. MYSc. 1 hit.
SUPFAMiSSF50084. SSF50084. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiView protein in PROSITE
PS50096. IQ. 1 hit.
PS51456. MYOSIN_MOTOR. 1 hit.
PS51844. SH3_LIKE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q61879-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQRTGLEDP ERYLFVDRAV IYNPATQADW TAKKLVWIPS ERHGFEAASI
60 70 80 90 100
KEERGDEVMV ELAENGKKAM VNKDDIQKMN PPKFSKVEDM AELTCLNEAS
110 120 130 140 150
VLHNLKDRYY SGLIYTYSGL FCVVINPYKN LPIYSENIIE MYRGKKRHEM
160 170 180 190 200
PPHIYAISES AYRCMLQDRE DQSILCTGES GAGKTENTKK VIQYLAHVAS
210 220 230 240 250
SHKGRKDHNI PGELERQLLQ ANPILESFGN AKTVKNDNSS RFGKFIRINF
260 270 280 290 300
DVTGYIVGAN IETYLLEKSR AVRQAKDERT FHIFYQLLSG AGEHLKSDLL
310 320 330 340 350
LEGFNNYRFL SNGYIPIPGQ QDKDNFQETM EAMHIMGFSH EEILSMLKVV
360 370 380 390 400
SSVLQFGNIS FKKERNTDQA SMPENTVAQK LCHLLGMNVM EFTRAILTPR
410 420 430 440 450
IKVGRDYVQK AQTKEQADFA VEALAKATYE RLFRWLVHRI NKALDRTKRQ
460 470 480 490 500
GASFIGILDI AGFEIFELNS FEQLCINYTN EKLQQLFNHT MFILEQEEYQ
510 520 530 540 550
REGIEWNFID FGLDLQPCID LIERPANPPG VLALLDEECW FPKATDKTFV
560 570 580 590 600
EKLVQEQGSH SKFQKPRQLK DKADFCIIHY AGKVDYKADE WLMKNMDPLN
610 620 630 640 650
DNVATLLHQS SDRFVAELWK DVDRIVGLDQ VTGMTETAFG SAYKTKKGMF
660 670 680 690 700
RTVGQLYKES LTKLMATLRN TNPNFVRCII PNHEKRAGKL DPHLVLDQLR
710 720 730 740 750
CNGVLEGIRI CRQGFPNRIV FQEFRQRYEI LTPNAIPKGF MDGKQACERM
760 770 780 790 800
IRALELDPNL YRIGQSKIFF RAGVLAHLEE ERDLKITDII IFFQAVCRGY
810 820 830 840 850
LARKAFAKKQ QQLSALKVLQ RNCAAYLKLR HWQWWRVFTK VKPLLQVTRQ
860 870 880 890 900
EEELQAKDEE LLKVKEKQTK VEGELEEMER KHQQLLEEKN ILAEQLQAET
910 920 930 940 950
ELFAEAEEMR ARLAAKKQEL EEILHDLESR VEEEEERNQI LQNEKKKMQA
960 970 980 990 1000
HIQDLEEQLD EEEGARQKLQ LEKVTAEAKI KKMEEEVLLL EDQNSKFIKE
1010 1020 1030 1040 1050
KKLMEDRIAE CSSQLAEEEE KAKNLAKIRN KQEVMISDLE ERLKKEEKTR
1060 1070 1080 1090 1100
QELEKAKRKL DGETTDLQDQ IAELQAQVDE LKVQLTKKEE ELQGALARGD
1110 1120 1130 1140 1150
DETLHKNNAL KVARELQAQI AELQEDFESE KASRNKAEKQ KRDLSEELEA
1160 1170 1180 1190 1200
LKTELEDTLD TTAAQQELRT KREQEVAELK KALEDETKNH EAQIQDMRQR
1210 1220 1230 1240 1250
HATALEELSE QLEQAKRFKA NLEKNKQGLE TDNKELACEV KVLQQVKAES
1260 1270 1280 1290 1300
EHKRKKLDAQ VQELHAKVSE GDRLRVELAE KANKLQNELD NVSTLLEEAE
1310 1320 1330 1340 1350
KKGIKFAKDA AGLESQLQDT QELLQEETRQ KLNLSSRIRQ LEEEKNSLQE
1360 1370 1380 1390 1400
QQEEEEEARK NLEKQVLALQ SQLADTKKKV DDDLGTIESL EEAKKKLLKD
1410 1420 1430 1440 1450
VEALSQRLEE KVLAYDKLEK TKNRLQQELD DLTVDLDHQR QIVSNLEKKQ
1460 1470 1480 1490 1500
KKFDQLLAEE KGISARYAEE RDRAEAEARE KETKALSLAR ALEEALEAKE
1510 1520 1530 1540 1550
EFERQNKQLR ADMEDLMSSK DDVGKNVHEL EKSKRALEQQ VEEMRTQLEE
1560 1570 1580 1590 1600
LEDELQATED AKLRLEVNMQ AMKAQFERDL QTRDEQNEEK KRLLLKQVRE
1610 1620 1630 1640 1650
LEAELEDERK QRALAVASKK KMEIDLKDLE AQIEAANKAR DEVIKQLRKL
1660 1670 1680 1690 1700
QAQMKDYQRE LEEARASRDE IFAQSKESEK KLKSLEAEIL QLQEELASSE
1710 1720 1730 1740 1750
RARRHAEQER DELADEIANS ASGKSALLDE KRRLEARIAQ LEEELEEEQS
1760 1770 1780 1790 1800
NMELLNDRFR KTTLQVDTLN TELAAERSAA QKSDNARQQL ERQNKELKAK
1810 1820 1830 1840 1850
LQELEGAVKS KFKATISALE AKIGQLEEQL EQEAKERAAA NKLVRRTEKK
1860 1870 1880 1890 1900
LKEIFMQVED ERRHADQYKE QMEKANARMK QLKRQLEEAE EEATRANASR
1910 1920 1930 1940 1950
RKLQRELDDA TEANEGLSRE VSTLKNRLRR GGPISFSSSR SGRRQLHIEG
1960 1970
ASLELSDDDT ESKTSDVNDT QPPQSE
Length:1,976
Mass (Da):228,996
Last modified:July 19, 2005 - v2
Checksum:iD00F922EB6682AEF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL645644, AL603662 Genomic DNA. Translation: CAI25576.1.
AL603662, AL645644 Genomic DNA. Translation: CAI25528.1.
BC089011 mRNA. Translation: AAH89011.1.
AH003264 Genomic DNA. Translation: AAA84879.1.
CCDSiCCDS24869.1.
RefSeqiNP_780469.1. NM_175260.2.
UniGeneiMm.218233.

Genome annotation databases

EnsembliENSMUST00000102611; ENSMUSP00000099671; ENSMUSG00000020900.
GeneIDi77579.
KEGGimmu:77579.
UCSCiuc007jny.1. mouse.

Similar proteinsi

Entry informationi

Entry nameiMYH10_MOUSE
AccessioniPrimary (citable) accession number: Q61879
Secondary accession number(s): Q5SV63
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: July 19, 2005
Last modified: November 22, 2017
This is version 165 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Represents a conventional non-muscle myosin. This protein should not be confused with the unconventional myosin-10 (MYO10).Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families