SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q61838

- A2M_MOUSE

UniProt

Q61838 - A2M_MOUSE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Alpha-2-macroglobulin

Gene
A2m, Pzp
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.

GO - Molecular functioni

  1. endopeptidase inhibitor activity Source: MGI
  2. serine-type endopeptidase inhibitor activity Source: UniProtKB-KW

GO - Biological processi

  1. embryo implantation Source: MGI
  2. negative regulation of endopeptidase activity Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Serine protease inhibitor

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-2-macroglobulin
Short name:
Alpha-2-M
Alternative name(s):
Pregnancy zone protein
Cleaved into the following 2 chains:
Gene namesi
Name:A2m
Synonyms:Pzp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 6

Organism-specific databases

MGIiMGI:87854. Pzp.

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: MGI
  2. extracellular space Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424 Reviewed predictionAdd
BLAST
Chaini25 – 14951471Alpha-2-macroglobulinPRO_0000338413Add
BLAST
Chaini25 – 12391215Alpha-2-macroglobulin 165 kDa subunitPRO_0000000056Add
BLAST
Chaini1240 – 1495256Alpha-2-macroglobulin 35 kDa subunitPRO_0000000057Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi48 ↔ 86 By similarity
Glycosylationi55 – 551N-linked (GlcNAc...) Reviewed prediction
Glycosylationi157 – 1571N-linked (GlcNAc...)2 Publications
Disulfide bondi249 ↔ 298 By similarity
Disulfide bondi267 ↔ 286 By similarity
Disulfide bondi277 – 277Interchain (with C-430) By similarity
Glycosylationi382 – 3821N-linked (GlcNAc...) Reviewed prediction
Glycosylationi405 – 4051N-linked (GlcNAc...)1 Publication
Glycosylationi412 – 4121N-linked (GlcNAc...)1 Publication
Disulfide bondi430 – 430Interchain (with C-277) By similarity
Disulfide bondi469 ↔ 562 By similarity
Glycosylationi568 – 5681N-linked (GlcNAc...)1 Publication
Disulfide bondi594 ↔ 783 By similarity
Disulfide bondi642 ↔ 689 By similarity
Disulfide bondi833 ↔ 861 By similarity
Disulfide bondi859 ↔ 895 By similarity
Glycosylationi881 – 8811N-linked (GlcNAc...)1 Publication
Disulfide bondi933 ↔ 1339 By similarity
Glycosylationi942 – 9421N-linked (GlcNAc...) Reviewed prediction
Cross-linki984 ↔ 987Isoglutamyl cysteine thioester (Cys-Gln) By similarity
Glycosylationi1003 – 10031N-linked (GlcNAc...)2 Publications
Disulfide bondi1092 ↔ 1140 By similarity
Glycosylationi1385 – 13851N-linked (GlcNAc...)2 Publications
Glycosylationi1443 – 14431N-linked (GlcNAc...)2 Publications

Keywords - PTMi

Disulfide bond, Glycoprotein, Thioester bond

Proteomic databases

MaxQBiQ61838.
PaxDbiQ61838.
PRIDEiQ61838.

PTM databases

PhosphoSiteiQ61838.

Expressioni

Tissue specificityi

Highest expression in liver, medium expression in ovary, heart and stomach. Low expression in lung, kidney and uterus. Protein found in plasma.1 Publication

Developmental stagei

Contrary to the rat protein, which is an acute phase protein, this protein is always present at high levels in circulation.

Gene expression databases

BgeeiQ61838.
CleanExiMM_A2M.
MM_PZP.
GenevestigatoriQ61838.

Interactioni

Protein-protein interaction databases

BioGridi197897. 2 interactions.
IntActiQ61838. 6 interactions.
MINTiMINT-1846187.

Structurei

3D structure databases

ProteinModelPortaliQ61838.
SMRiQ61838. Positions 124-225, 1357-1486.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni686 – 74459Bait regionAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Bait region, Signal

Phylogenomic databases

eggNOGiCOG2373.
GeneTreeiENSGT00740000115177.
HOGENOMiHOG000220939.
HOVERGENiHBG000039.

Family and domain databases

Gene3Di1.50.10.20. 1 hit.
2.60.40.690. 1 hit.
InterProiIPR009048. A-macroglobulin_rcpt-bd.
IPR011626. A2M_comp.
IPR002890. A2M_N.
IPR011625. A2M_N_2.
IPR014756. Ig_E-set.
IPR001599. Macroglobln_a2.
IPR019742. MacrogloblnA2_CS.
IPR019565. MacrogloblnA2_thiol-ester-bond.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
[Graphical view]
PfamiPF00207. A2M. 1 hit.
PF07678. A2M_comp. 1 hit.
PF01835. A2M_N. 1 hit.
PF07703. A2M_N_2. 1 hit.
PF07677. A2M_recep. 1 hit.
PF10569. Thiol-ester_cl. 1 hit.
[Graphical view]
SUPFAMiSSF48239. SSF48239. 1 hit.
SSF49410. SSF49410. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEiPS00477. ALPHA_2_MACROGLOBULIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q61838-1 [UniParc]FASTAAdd to Basket

« Hide

MRRNQLPTPA FLLLFLLLPR DATTATAKPQ YVVLVPSEVY SGVPEKACVS     50
LNHVNETVML SLTLEYAMQQ TKLLTDQAVD KDSFYCSPFT ISGSPLPYTF 100
ITVEIKGPTQ RFIKKKSIQI IKAESPVFVQ TDKPIYKPGQ IVKFRVVSVD 150
ISFRPLNETF PVVYIETPKR NRIFQWQNIH LAGGLHQLSF PLSVEPALGI 200
YKVVVQKDSG KKIEHSFEVK EYVLPKFEVI IKMQKTMAFL EEELPITACG 250
VYTYGKPVPG LVTLRVCRKY SRYRSTCHNQ NSMSICEEFS QQADDKGCFR 300
QVVKTKVFQL RQKGHDMKIE VEAKIKEEGT GIELTGIGSC EIANALSKLK 350
FTKVNTNYRP GLPFSGQVLL VDEKGKPIPN KNITSVVSPL GYLSIFTTDE 400
HGLANISIDT SNFTAPFLRV VVTYKQNHVC YDNWWLDEFH TQADHSATLV 450
FSPSQSYIQL ELVFGTLACG QTQEIRIHYL LNEDIMKNEK DLTFYYLIKA 500
RGSIFNLGSH VLSLEQGNMK GVFSLPIQVE PGMAPEAQLL IYAILPNEEL 550
VADAQNFEIE KCFANKVNLS FPSAQSLPAS DTHLKVKAAP LSLCALTAVD 600
QSVLLLKPEA KLSPQSIYNL LPGKTVQGAF FGVPVYKDHE NCISGEDITH 650
NGIVYTPKHS LGDNDAHSIF QSVGINIFTN SKIHKPRFCQ EFQHYPAMGG 700
VAPQALAVAA SGPGSSFRAM GVPMMGLDYS DEINQVVEVR ETVRKYFPET 750
WIWDLVPLDV SGDGELAVKV PDTITEWKAS AFCLSGTTGL GLSSTISLQA 800
FQPFFLELTL PYSVVRGEAF TLKATVLNYM SHCIQIRVDL EISPDFLAVP 850
VGGHENSHCI CGNERKTVSW AVTPKSLGEV NFTATAEALQ SPELCGNKLT 900
EVPALVHKDT VVKSVIVEPE GIEKEQTYNT LLCPQDTELQ DNWSLELPPN 950
VVEGSARATH SVLGDILGSA MQNLQNLLQM PYGCGEQNMV LFVPNIYVLN 1000
YLNETQQLTE AIKSKAINYL ISGYQRQLNY QHSDGSYSTF GNHGGGNTPG 1050
NTWLTAFVLK AFAQAQSHIF IEKTHITNAF NWLSMKQKEN GCFQQSGYLL 1100
NNAMKGGVDD EVTLSAYITI ALLEMPLPVT HSAVRNALFC LETAWASISQ 1150
SQESHVYTKA LLAYAFALAG NKAKRSELLE SLNKDAVKEE DSLHWQRPGD 1200
VQKVKALSFY QPRAPSAEVE MTAYVLLAYL TSESSRPTRD LSSSDLSTAS 1250
KIVKWISKQQ NSHGGFSSTQ DTVVALQALS KYGAATFTRS QKEVLVTIES 1300
SGTFSKTFHV NSGNRLLLQE VRLPDLPGNY VTKGSGSGCV YLQTSLKYNI 1350
LPVADGKAPF ALQVNTLPLN FDKAGDHRTF QIRINVSYTG ERPSSNMVIV 1400
DVKMVSGFIP MKPSVKKLQD QPNIQRTEVN TNHVLIYIEK LTNQTLGFSF 1450
AVEQDIPVKN LKPAPIKVYD YYETDEFTVE EYSAPFSDGS EQGNA 1495
Length:1,495
Mass (Da):165,853
Last modified:June 28, 2011 - v3
Checksum:i5D060D6C639DF3BC
GO

Sequence cautioni

The sequence AAA39508.1 differs from that shown. Reason: Frameshift at positions 41 and 47.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti43 – 431V → I in AAA39508. 1 Publication
Sequence conflicti43 – 431V → I in AAH57983. 1 Publication
Sequence conflicti43 – 431V → I in AAA87890. 1 Publication
Sequence conflicti91 – 911I → V in AAA87890. 1 Publication
Sequence conflicti142 – 1443VKF → GII in AAA87890. 1 Publication
Sequence conflicti160 – 1612FP → VS in AAA87890. 1 Publication
Sequence conflicti287 – 2871E → A in AAA39508. 1 Publication
Sequence conflicti300 – 3001R → S in AAA39508. 1 Publication
Sequence conflicti311 – 3111R → S in AAA39508. 1 Publication
Sequence conflicti491 – 4911D → T in AAA39508. 1 Publication
Sequence conflicti505 – 5051F → G in AAA39508. 1 Publication
Sequence conflicti541 – 5411I → V in AAH57983. 1 Publication
Sequence conflicti792 – 7921L → S in AAA39508. 1 Publication
Sequence conflicti884 – 8841A → R in AAA39508. 1 Publication
Sequence conflicti890 – 8923QSP → ESQ in AAA39508. 1 Publication
Sequence conflicti943 – 9431W → S in AAA39508. 1 Publication
Sequence conflicti1133 – 11331A → V in AAH57983. 1 Publication
Sequence conflicti1263 – 12686HGGFSS → DGGLLL in AAA39508. 1 Publication
Sequence conflicti1284 – 12841A → S in AAA39508. 1 Publication
Sequence conflicti1298 – 12992IE → SR in AAA39508. 1 Publication
Sequence conflicti1375 – 13751G → E in AAA39508. 1 Publication
Sequence conflicti1417 – 14171K → R in AAA39508. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M93264 mRNA. Translation: AAA39508.1. Frameshift.
AC123060 Genomic DNA. No translation available.
BC057983 mRNA. Translation: AAH57983.1.
U06977 Genomic DNA. Translation: AAA87890.1.
CCDSiCCDS39650.1.
PIRiS27001.
RefSeqiNP_031402.3. NM_007376.4.
UniGeneiMm.260144.

Genome annotation databases

EnsembliENSMUST00000112132; ENSMUSP00000107760; ENSMUSG00000030359.
GeneIDi11287.
KEGGimmu:11287.
UCSCiuc009eef.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M93264 mRNA. Translation: AAA39508.1 . Frameshift.
AC123060 Genomic DNA. No translation available.
BC057983 mRNA. Translation: AAH57983.1 .
U06977 Genomic DNA. Translation: AAA87890.1 .
CCDSi CCDS39650.1.
PIRi S27001.
RefSeqi NP_031402.3. NM_007376.4.
UniGenei Mm.260144.

3D structure databases

ProteinModelPortali Q61838.
SMRi Q61838. Positions 124-225, 1357-1486.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 197897. 2 interactions.
IntActi Q61838. 6 interactions.
MINTi MINT-1846187.

PTM databases

PhosphoSitei Q61838.

Proteomic databases

MaxQBi Q61838.
PaxDbi Q61838.
PRIDEi Q61838.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000112132 ; ENSMUSP00000107760 ; ENSMUSG00000030359 .
GeneIDi 11287.
KEGGi mmu:11287.
UCSCi uc009eef.1. mouse.

Organism-specific databases

CTDi 5858.
MGIi MGI:87854. Pzp.

Phylogenomic databases

eggNOGi COG2373.
GeneTreei ENSGT00740000115177.
HOGENOMi HOG000220939.
HOVERGENi HBG000039.

Miscellaneous databases

NextBioi 278574.
SOURCEi Search...

Gene expression databases

Bgeei Q61838.
CleanExi MM_A2M.
MM_PZP.
Genevestigatori Q61838.

Family and domain databases

Gene3Di 1.50.10.20. 1 hit.
2.60.40.690. 1 hit.
InterProi IPR009048. A-macroglobulin_rcpt-bd.
IPR011626. A2M_comp.
IPR002890. A2M_N.
IPR011625. A2M_N_2.
IPR014756. Ig_E-set.
IPR001599. Macroglobln_a2.
IPR019742. MacrogloblnA2_CS.
IPR019565. MacrogloblnA2_thiol-ester-bond.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
[Graphical view ]
Pfami PF00207. A2M. 1 hit.
PF07678. A2M_comp. 1 hit.
PF01835. A2M_N. 1 hit.
PF07703. A2M_N_2. 1 hit.
PF07677. A2M_recep. 1 hit.
PF10569. Thiol-ester_cl. 1 hit.
[Graphical view ]
SUPFAMi SSF48239. SSF48239. 1 hit.
SSF49410. SSF49410. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEi PS00477. ALPHA_2_MACROGLOBULIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The primary sequence and the subunit structure of mouse alpha-2-macroglobulin, deduced from protein sequencing of the isolated subunits and from molecular cloning of the cDNA."
    van Leuven F., Torrekens S., Overbergh L., Lorent K., de Strooper B., van den Berghe H.
    Eur. J. Biochem. 210:319-327(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 25-44 AND 1240-1259.
    Tissue: Liver.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Liver.
  4. "Molecular cloning of the mouse gene coding for alpha 2-macroglobulin and targeting of the gene in embryonic stem cells."
    Umans L., Serneels L., Hilliker C., Stas L., Overbergh L., de Strooper B., van Leuven F., van den Berghe H.
    Genomics 22:519-529(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-161.
    Strain: 129/J.
  5. "Characterization of a murine alpha 2 macroglobulin gene expressed in reproductive and cardiovascular tissue."
    He H., McCartney D.J., Wei Q., Esadeg S., Zhang J., Foster R.A., Hayes M.A., Tayade C., Van Leuven F., Croy B.A.
    Biol. Reprod. 72:266-275(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
    Strain: C57BL/6.
    Tissue: Placenta.
  6. "Proteome-wide characterization of N-glycosylation events by diagonal chromatography."
    Ghesquiere B., Van Damme J., Martens L., Vandekerckhove J., Gevaert K.
    J. Proteome Res. 5:2438-2447(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-157; ASN-405; ASN-412; ASN-1003; ASN-1385 AND ASN-1443.
    Strain: C57BL/6.
    Tissue: Plasma.
  7. "Enhanced analysis of the mouse plasma proteome using cysteine-containing tryptic glycopeptides."
    Bernhard O.K., Kapp E.A., Simpson R.J.
    J. Proteome Res. 6:987-995(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-157; ASN-568; ASN-881; ASN-1003; ASN-1385 AND ASN-1443.
    Strain: C57BL/6.
    Tissue: Plasma.

Entry informationi

Entry nameiA2M_MOUSE
AccessioniPrimary (citable) accession number: Q61838
Secondary accession number(s): E9QPW0, Q60628, Q6PEM2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: June 28, 2011
Last modified: July 9, 2014
This is version 121 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi