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Protein

Pregnancy zone protein

Gene

Pzp

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region, a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.

GO - Molecular functioni

GO - Biological processi

  • embryo implantation Source: MGI

Keywordsi

Molecular functionProtease inhibitor, Serine protease inhibitor

Names & Taxonomyi

Protein namesi
Recommended name:
Pregnancy zone protein
Alternative name(s):
Alpha-2-macroglobulin
Short name:
Alpha-2-M
Cleaved into the following 2 chains:
Gene namesi
Name:Pzp
Synonyms:A2m
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:87854 Pzp

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
ChainiPRO_000033841325 – 1495Pregnancy zone proteinAdd BLAST1471
ChainiPRO_000000005625 – 1239Alpha-2-macroglobulin 165 kDa subunitAdd BLAST1215
ChainiPRO_00000000571240 – 1495Alpha-2-macroglobulin 35 kDa subunitAdd BLAST256

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi48 ↔ 86By similarity
Glycosylationi55N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi157N-linked (GlcNAc...) asparagine2 Publications1
Disulfide bondi249 ↔ 298By similarity
Disulfide bondi267 ↔ 286By similarity
Disulfide bondi277Interchain (with C-430)By similarity
Glycosylationi382N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi405N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi412N-linked (GlcNAc...) asparagine1 Publication1
Disulfide bondi430Interchain (with C-277)By similarity
Disulfide bondi469 ↔ 562By similarity
Glycosylationi568N-linked (GlcNAc...) asparagine1 Publication1
Disulfide bondi594 ↔ 783By similarity
Disulfide bondi642 ↔ 689By similarity
Disulfide bondi833 ↔ 861By similarity
Disulfide bondi859 ↔ 895By similarity
Glycosylationi881N-linked (GlcNAc...) asparagine1 Publication1
Disulfide bondi933 ↔ 1339By similarity
Glycosylationi942N-linked (GlcNAc...) asparagineSequence analysis1
Cross-linki984 ↔ 987Isoglutamyl cysteine thioester (Cys-Gln)By similarity
Glycosylationi1003N-linked (GlcNAc...) asparagine2 Publications1
Disulfide bondi1092 ↔ 1140By similarity
Glycosylationi1385N-linked (GlcNAc...) asparagine2 Publications1
Glycosylationi1443N-linked (GlcNAc...) asparagine2 Publications1

Keywords - PTMi

Disulfide bond, Glycoprotein, Thioester bond

Proteomic databases

MaxQBiQ61838
PaxDbiQ61838
PeptideAtlasiQ61838
PRIDEiQ61838

PTM databases

CarbonylDBiQ61838
GlyConnecti675
iPTMnetiQ61838
PhosphoSitePlusiQ61838
SwissPalmiQ61838

Expressioni

Tissue specificityi

Highest expression in liver, medium expression in ovary, heart and stomach. Low expression in lung, kidney and uterus. Protein found in plasma.1 Publication

Developmental stagei

Unlike the rat protein, which is an acute phase protein, this protein is always in circulation at high levels.

Gene expression databases

BgeeiENSMUSG00000030359
CleanExiMM_A2M
MM_PZP
ExpressionAtlasiQ61838 baseline and differential
GenevisibleiQ61838 MM

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi197897, 3 interactors
IntActiQ61838, 6 interactors
MINTiQ61838
STRINGi10090.ENSMUSP00000107760

Structurei

3D structure databases

ProteinModelPortaliQ61838
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni686 – 744Bait regionAdd BLAST59

Sequence similaritiesi

Keywords - Domaini

Bait region, Signal

Phylogenomic databases

eggNOGiENOG410KCRJ Eukaryota
COG2373 LUCA
GeneTreeiENSGT00760000118982
HOGENOMiHOG000220939
HOVERGENiHBG000039
InParanoidiQ61838
OMAiSIHWQRP

Family and domain databases

Gene3Di2.60.40.10, 2 hits
2.60.40.690, 1 hit
InterProiView protein in InterPro
IPR009048 A-macroglobulin_rcpt-bd
IPR036595 A-macroglobulin_rcpt-bd_sf
IPR011626 A2M_comp
IPR002890 A2M_N
IPR011625 A2M_N_2
IPR013783 Ig-like_fold
IPR014756 Ig_E-set
IPR001599 Macroglobln_a2
IPR019742 MacrogloblnA2_CS
IPR019565 MacrogloblnA2_thiol-ester-bond
IPR008930 Terpenoid_cyclase/PrenylTrfase
PfamiView protein in Pfam
PF00207 A2M, 1 hit
PF07678 A2M_comp, 1 hit
PF01835 A2M_N, 1 hit
PF07703 A2M_N_2, 1 hit
PF07677 A2M_recep, 1 hit
PF10569 Thiol-ester_cl, 1 hit
SMARTiView protein in SMART
SM01360 A2M, 1 hit
SM01359 A2M_N_2, 1 hit
SM01361 A2M_recep, 1 hit
SUPFAMiSSF48239 SSF48239, 1 hit
SSF49410 SSF49410, 1 hit
SSF81296 SSF81296, 1 hit
PROSITEiView protein in PROSITE
PS00477 ALPHA_2_MACROGLOBULIN, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q61838-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRRNQLPTPA FLLLFLLLPR DATTATAKPQ YVVLVPSEVY SGVPEKACVS
60 70 80 90 100
LNHVNETVML SLTLEYAMQQ TKLLTDQAVD KDSFYCSPFT ISGSPLPYTF
110 120 130 140 150
ITVEIKGPTQ RFIKKKSIQI IKAESPVFVQ TDKPIYKPGQ IVKFRVVSVD
160 170 180 190 200
ISFRPLNETF PVVYIETPKR NRIFQWQNIH LAGGLHQLSF PLSVEPALGI
210 220 230 240 250
YKVVVQKDSG KKIEHSFEVK EYVLPKFEVI IKMQKTMAFL EEELPITACG
260 270 280 290 300
VYTYGKPVPG LVTLRVCRKY SRYRSTCHNQ NSMSICEEFS QQADDKGCFR
310 320 330 340 350
QVVKTKVFQL RQKGHDMKIE VEAKIKEEGT GIELTGIGSC EIANALSKLK
360 370 380 390 400
FTKVNTNYRP GLPFSGQVLL VDEKGKPIPN KNITSVVSPL GYLSIFTTDE
410 420 430 440 450
HGLANISIDT SNFTAPFLRV VVTYKQNHVC YDNWWLDEFH TQADHSATLV
460 470 480 490 500
FSPSQSYIQL ELVFGTLACG QTQEIRIHYL LNEDIMKNEK DLTFYYLIKA
510 520 530 540 550
RGSIFNLGSH VLSLEQGNMK GVFSLPIQVE PGMAPEAQLL IYAILPNEEL
560 570 580 590 600
VADAQNFEIE KCFANKVNLS FPSAQSLPAS DTHLKVKAAP LSLCALTAVD
610 620 630 640 650
QSVLLLKPEA KLSPQSIYNL LPGKTVQGAF FGVPVYKDHE NCISGEDITH
660 670 680 690 700
NGIVYTPKHS LGDNDAHSIF QSVGINIFTN SKIHKPRFCQ EFQHYPAMGG
710 720 730 740 750
VAPQALAVAA SGPGSSFRAM GVPMMGLDYS DEINQVVEVR ETVRKYFPET
760 770 780 790 800
WIWDLVPLDV SGDGELAVKV PDTITEWKAS AFCLSGTTGL GLSSTISLQA
810 820 830 840 850
FQPFFLELTL PYSVVRGEAF TLKATVLNYM SHCIQIRVDL EISPDFLAVP
860 870 880 890 900
VGGHENSHCI CGNERKTVSW AVTPKSLGEV NFTATAEALQ SPELCGNKLT
910 920 930 940 950
EVPALVHKDT VVKSVIVEPE GIEKEQTYNT LLCPQDTELQ DNWSLELPPN
960 970 980 990 1000
VVEGSARATH SVLGDILGSA MQNLQNLLQM PYGCGEQNMV LFVPNIYVLN
1010 1020 1030 1040 1050
YLNETQQLTE AIKSKAINYL ISGYQRQLNY QHSDGSYSTF GNHGGGNTPG
1060 1070 1080 1090 1100
NTWLTAFVLK AFAQAQSHIF IEKTHITNAF NWLSMKQKEN GCFQQSGYLL
1110 1120 1130 1140 1150
NNAMKGGVDD EVTLSAYITI ALLEMPLPVT HSAVRNALFC LETAWASISQ
1160 1170 1180 1190 1200
SQESHVYTKA LLAYAFALAG NKAKRSELLE SLNKDAVKEE DSLHWQRPGD
1210 1220 1230 1240 1250
VQKVKALSFY QPRAPSAEVE MTAYVLLAYL TSESSRPTRD LSSSDLSTAS
1260 1270 1280 1290 1300
KIVKWISKQQ NSHGGFSSTQ DTVVALQALS KYGAATFTRS QKEVLVTIES
1310 1320 1330 1340 1350
SGTFSKTFHV NSGNRLLLQE VRLPDLPGNY VTKGSGSGCV YLQTSLKYNI
1360 1370 1380 1390 1400
LPVADGKAPF ALQVNTLPLN FDKAGDHRTF QIRINVSYTG ERPSSNMVIV
1410 1420 1430 1440 1450
DVKMVSGFIP MKPSVKKLQD QPNIQRTEVN TNHVLIYIEK LTNQTLGFSF
1460 1470 1480 1490
AVEQDIPVKN LKPAPIKVYD YYETDEFTVE EYSAPFSDGS EQGNA
Length:1,495
Mass (Da):165,853
Last modified:June 28, 2011 - v3
Checksum:i5D060D6C639DF3BC
GO

Sequence cautioni

The sequence AAA39508 differs from that shown. Reason: Frameshift at positions 41 and 47.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti43V → I in AAA39508 (PubMed:1280217).Curated1
Sequence conflicti43V → I in AAH57983 (PubMed:15489334).Curated1
Sequence conflicti43V → I in AAA87890 (PubMed:7528166).Curated1
Sequence conflicti91I → V in AAA87890 (PubMed:7528166).Curated1
Sequence conflicti142 – 144VKF → GII in AAA87890 (PubMed:7528166).Curated3
Sequence conflicti160 – 161FP → VS in AAA87890 (PubMed:7528166).Curated2
Sequence conflicti287E → A in AAA39508 (PubMed:1280217).Curated1
Sequence conflicti300R → S in AAA39508 (PubMed:1280217).Curated1
Sequence conflicti311R → S in AAA39508 (PubMed:1280217).Curated1
Sequence conflicti491D → T in AAA39508 (PubMed:1280217).Curated1
Sequence conflicti505F → G in AAA39508 (PubMed:1280217).Curated1
Sequence conflicti541I → V in AAH57983 (PubMed:15489334).Curated1
Sequence conflicti792L → S in AAA39508 (PubMed:1280217).Curated1
Sequence conflicti884A → R in AAA39508 (PubMed:1280217).Curated1
Sequence conflicti890 – 892QSP → ESQ in AAA39508 (PubMed:1280217).Curated3
Sequence conflicti943W → S in AAA39508 (PubMed:1280217).Curated1
Sequence conflicti1133A → V in AAH57983 (PubMed:15489334).Curated1
Sequence conflicti1263 – 1268HGGFSS → DGGLLL in AAA39508 (PubMed:1280217).Curated6
Sequence conflicti1284A → S in AAA39508 (PubMed:1280217).Curated1
Sequence conflicti1298 – 1299IE → SR in AAA39508 (PubMed:1280217).Curated2
Sequence conflicti1375G → E in AAA39508 (PubMed:1280217).Curated1
Sequence conflicti1417K → R in AAA39508 (PubMed:1280217).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M93264 mRNA Translation: AAA39508.1 Frameshift.
AC123060 Genomic DNA No translation available.
BC057983 mRNA Translation: AAH57983.1
U06977 Genomic DNA Translation: AAA87890.1
CCDSiCCDS39650.1
PIRiS27001
UniGeneiMm.260144

Genome annotation databases

EnsembliENSMUST00000112132; ENSMUSP00000107760; ENSMUSG00000030359
UCSCiuc009eef.1 mouse

Similar proteinsi

Entry informationi

Entry nameiPZP_MOUSE
AccessioniPrimary (citable) accession number: Q61838
Secondary accession number(s): E9QPW0, Q60628, Q6PEM2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: June 28, 2011
Last modified: April 25, 2018
This is version 146 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health