Q61827 (MAFK_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcription factor MafK

Alternative name(s):
Erythroid transcription factor NF-E2 p18 subunit

Gene names

Name:	Mafk
Synonyms:	Nfe2u

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	156 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Since they lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves. However, they seem to serve as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins and recruiting them to specific DNA-binding sites. Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NF-E2 transcription factor.
Subunit structure	Homodimer or heterodimer. It can form high affinity heterodimers with members of the CNC-bZIP family such as NFE2, NFE2L1/NRF1, NFE2L2/NRF2 and NFE2L3/NRF3 By similarity.
Subcellular location	Nucleus.
Tissue specificity	Highly expressed in heart, skeletal muscle and placenta. Also expressed in erythroid cells.
Sequence similarities	Belongs to the bZIP family. Maf subfamily. Contains 1 bZIP domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding
Molecular function	Repressor
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleoplasm Traceable author statement. Source: Reactome
Molecular function	protein binding Inferred from physical interaction. Source: BHF-UCL sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding transcription factor activity Inferred from direct assay. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 156	156	Transcription factor MafK		PRO_0000076504
Regions
	Domain	86 – 114	29	Leucine-zipper
	DNA binding	51 – 76	26	Basic motif
Amino acid modifications
	Modified residue	25	1	Phosphoserine By similarity
Experimental info
	Sequence conflict	36 – 37	2	EL → DV in AAC52132. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q61827 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 39F98F8A257F46CB	FASTA	156	17,537
	10 20 30 40 50 60 MTTNPKPNKA LKVKKEAGEN APVLSDDELV SMSVRELNQH LRGLTKEEVT RLKQRRRTLK 70 80 90 100 110 120 NRGYAASCRI KRVTQKEELE RQRVELQQEV EKLARENSSM RLELDALRSK YEALQTFART 130 140 150 VARGPVTPTK VATTSVITIV KSAELSSTSV PFSAAS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Activity and expression of murine small Maf family protein MafK." Igarashi K., Itoh K., Motohashi H., Hayashi N., Matuzaki Y., Nakauchi H., Nishizawa M., Yamamoto M. J. Biol. Chem. 270:7615-7624(1995) [PubMed: 7706310] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[2]	"The ubiquitous subunit of erythroid transcription factor NF-E2 is a small basic-leucine zipper protein related to the v-maf oncogene." Andrews N.C., Kotkow K.J., Ney P.A., Erdjument-Bromage H., Tempst P., Orkin S.H. Proc. Natl. Acad. Sci. U.S.A. 90:11488-11492(1993) [PubMed: 8265578] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: DBA. Tissue: Hematopoietic.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Salivary gland.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D42124 mRNA. Translation: BAA07704.1. U01036 mRNA. Translation: AAC52132.1. BC014295 mRNA. Translation: AAH14295.1.
IPI	IPI00126148.
PIR	A49391. A56124.
RefSeq	NP_034887.1. NM_010757.2.
UniGene	Mm.157313.
3D structure databases
ProteinModelPortal	Q61827.
SMR	Q61827. Positions 24-111.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-46345N.
STRING	Q61827.
PTM databases
PhosphoSite	Q61827.
Proteomic databases
PRIDE	Q61827.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000018287; ENSMUSP00000018287; ENSMUSG00000018143. ENSMUST00000110836; ENSMUSP00000106460; ENSMUSG00000018143.
GeneID	17135.
KEGG	mmu:17135.
Organism-specific databases
CTD	7975.
MGI	MGI:99951. Mafk.
Phylogenomic databases
GeneTree	ENSGT00550000074549.
HOGENOM	HBG715194.
HOVERGEN	HBG001725.
InParanoid	Q61827.
OMA	RGPVTPT.
OrthoDB	EOG4X3H2R.
PhylomeDB	Q61827.
Gene expression databases
ArrayExpress	Q61827.
Bgee	Q61827.
Genevestigator	Q61827.
GermOnline	ENSMUSG00000018143. Mus musculus.
Family and domain databases
InterPro	IPR004827. bZIP. IPR004826. bZIP_Maf. IPR008917. Euk_TF_DNA-bd. IPR024874. Transciption_factor_Maf. [Graphical view]
Gene3D	G3DSA:1.10.880.10. G3DSA:1.10.880.10. 1 hit.
KO	K09037.
PANTHER	PTHR10129. PTHR10129. 1 hit.
Pfam	PF03131. bZIP_Maf. 1 hit. [Graphical view]
SMART	SM00338. BRLZ. 1 hit. [Graphical view]
SUPFAM	SSF47454. Euk_transcr_DNA. 1 hit.
PROSITE	PS50217. BZIP. 1 hit. PS00036. BZIP_BASIC. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	291344.
SOURCE	Search...

Entry information

Entry name

MAFK_MOUSE

Accession

Primary (citable) accession number: Q61827
Secondary accession number(s): Q60600

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	January 25, 2012
This is version 92 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program