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Q61826

- MADCA_MOUSE

UniProt

Q61826 - MADCA_MOUSE

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Protein

Mucosal addressin cell adhesion molecule 1

Gene

Madcam1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Cell adhesion leukocyte receptor expressed by mucosal venules, helps to direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It can bind both the integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes. Both isoform 1 and isoform 2 can adhere to integrin alpha-4/beta-7. Isoform 2, lacking the mucin-like domain, may be specialized in supporting integrin alpha-4/beta-7-dependent adhesion strengthening, independent of L-selectin binding.2 Publications

GO - Biological processi

  1. cell adhesion Source: UniProtKB-KW
  2. keratinocyte differentiation Source: MGI
  3. leukocyte migration Source: MGI
Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Names & Taxonomyi

Protein namesi
Recommended name:
Mucosal addressin cell adhesion molecule 1
Short name:
MAdCAM-1
Short name:
mMAdCAM-1
Gene namesi
Name:Madcam1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Unplaced

Organism-specific databases

MGIiMGI:103579. Madcam1.

Subcellular locationi

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Involvement in diseasei

Absence of Madcam1 in the spleen has been found in aly/aly mice, but normal expression is found in intestinal venules. This aberrant expression is a secondary defect and not the direct cause of aly alymphoplasia, an autosomal recessive mutation which induces total aplasia of lymph nodes and Peyer patches.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Sequence AnalysisAdd
BLAST
Chaini22 – 405384Mucosal addressin cell adhesion molecule 1PRO_0000014854Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi45 ↔ 91By similarity
Disulfide bondi49 ↔ 95By similarity
Disulfide bondi132 ↔ 200By similarity
Glycosylationi230 – 2301N-linked (GlcNAc...)Sequence Analysis
Glycosylationi253 – 2531N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi293 ↔ 341Sequence Analysis
Glycosylationi361 – 3611N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

O-glycosylated; contains syalic acid. The Ser/Thr-rich mucin-like domain may provide possible sites for O-glycosylation.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiQ61826.

Expressioni

Tissue specificityi

Highly expressed on high endothelial venules (HEV) of organized intestinal lymphoid tissues like the Peyer patches and mesenteric lymph nodes, and in the lamina propria of the intestine. Some expression found in the spleen, and low levels of expression in the peripheral lymph nodes and the lactating mammary gland. No expression was detected in the liver, kidneys, lungs or in normal brain. Expressed as well in brain endothelioma cells, and mucosal tissues which are in a chronic state of inflammation, such as inflammed pancreas.3 Publications

Inductioni

By TNF-alpha, and chronic inflammation.2 Publications

Gene expression databases

GenevestigatoriQ61826.

Interactioni

Subunit structurei

Homodimer.Curated

Structurei

3D structure databases

ProteinModelPortaliQ61826.
SMRiQ61826. Positions 22-227.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini22 – 364343ExtracellularSequence AnalysisAdd
BLAST
Topological domaini386 – 40520CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei365 – 38521HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini22 – 10988Ig-like 1Add
BLAST
Domaini110 – 227118Ig-like 2Add
BLAST
Domaini258 – 357100Ig-like 3Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni221 – 25737Mucin-likeAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG26033.
HOGENOMiHOG000060103.
HOVERGENiHBG052394.
InParanoidiQ61826.
KOiK06779.
PhylomeDBiQ61826.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
InterProiIPR015169. Adhes-Ig-like.
IPR013783. Ig-like_fold.
[Graphical view]
PfamiPF09085. Adhes-Ig_like. 1 hit.
[Graphical view]
ProDomiPD336606. Adhes-Ig-like. 1 hit.
[Graphical view] [Entries sharing at least one domain]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q61826-1) [UniParc]FASTAAdd to Basket

Also known as: Long, MadCAM-384

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MESILALLLA LALVPYQLSR GQSFQVNPPE SEVAVAMGTS LQITCSMSCD
60 70 80 90 100
EGVARVHWRG LDTSLGSVQT LPGSSILSVR GMLSDTGTPV CVGSCGSRSF
110 120 130 140 150
QHSVKILVYA FPDQLVVSPE FLVPGQDQVV SCTAHNIWPA DPNSLSFALL
160 170 180 190 200
LGEQRLEGAQ ALEPEQEEEI QEAEGTPLFR MTQRWRLPSL GTPAPPALHC
210 220 230 240 250
QVTMQLPKLV LTHRKEIPVL QSQTSPKPPN TTSAEPYILT SSSTAEAVST
260 270 280 290 300
GLNITTLPSA PPYPKLSPRT LSSEGPCRPK IHQDLEAGWE LLCEASCGPG
310 320 330 340 350
VTVRWTLAPG DLATYHKREA GAQAWLSVLP PGPMVEGWFQ CRQDPGGEVT
360 370 380 390 400
NLYVPGQVTP NSSSTVVLWI GSLVLGLLAL VFLAYRLWKC YRPGPRPDTS

SCTHL
Length:405
Mass (Da):43,652
Last modified:February 2, 2004 - v2
Checksum:i4F2D08418EA51980
GO
Isoform 2 (identifier: Q61826-2) [UniParc]FASTAAdd to Basket

Also known as: Short, MadCAM-240

The sequence of this isoform differs from the canonical sequence as follows:
     219-363: VLQSQTSPKP...VPGQVTPNSS → A

Show »
Length:261
Mass (Da):28,357
Checksum:iBD1F0984799DF3B0
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti6 – 61A → S in BAA23364. (PubMed:9316640)Curated
Sequence conflicti10 – 101A → S(PubMed:9316640)Curated
Sequence conflicti12 – 121A → S(PubMed:9316640)Curated
Sequence conflicti61 – 611L → R(PubMed:8502297)Curated
Sequence conflicti61 – 611L → R(PubMed:9316640)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei219 – 363145VLQSQ…TPNSS → A in isoform 2. 2 PublicationsVSP_050423Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L21203 mRNA. Translation: AAA39485.1.
U14729, U14552 Genomic DNA. Translation: AAA81639.1.
U14729, U14552 Genomic DNA. Translation: AAA81638.1.
S80258 mRNA. Translation: AAB35609.1.
D50434 Genomic DNA. Translation: BAA23364.1.
BC024372 mRNA. Translation: AAH24372.1.
CCDSiCCDS23981.1. [Q61826-1]
PIRiI48645.
S33601.
RefSeqiXP_006513356.1. XM_006513293.1. [Q61826-2]
UniGeneiMm.391556.

Genome annotation databases

GeneIDi17123.
KEGGimmu:17123.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L21203 mRNA. Translation: AAA39485.1 .
U14729 , U14552 Genomic DNA. Translation: AAA81639.1 .
U14729 , U14552 Genomic DNA. Translation: AAA81638.1 .
S80258 mRNA. Translation: AAB35609.1 .
D50434 Genomic DNA. Translation: BAA23364.1 .
BC024372 mRNA. Translation: AAH24372.1 .
CCDSi CCDS23981.1. [Q61826-1 ]
PIRi I48645.
S33601.
RefSeqi XP_006513356.1. XM_006513293.1. [Q61826-2 ]
UniGenei Mm.391556.

3D structure databases

ProteinModelPortali Q61826.
SMRi Q61826. Positions 22-227.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi Q61826.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 17123.
KEGGi mmu:17123.

Organism-specific databases

CTDi 8174.
MGIi MGI:103579. Madcam1.

Phylogenomic databases

eggNOGi NOG26033.
HOGENOMi HOG000060103.
HOVERGENi HBG052394.
InParanoidi Q61826.
KOi K06779.
PhylomeDBi Q61826.

Miscellaneous databases

PROi Q61826.
SOURCEi Search...

Gene expression databases

Genevestigatori Q61826.

Family and domain databases

Gene3Di 2.60.40.10. 2 hits.
InterProi IPR015169. Adhes-Ig-like.
IPR013783. Ig-like_fold.
[Graphical view ]
Pfami PF09085. Adhes-Ig_like. 1 hit.
[Graphical view ]
ProDomi PD336606. Adhes-Ig-like. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "MAdCAM-1 has homology to immunoglobulin and mucin-like adhesion receptors and to IgA1."
    Briskin M.J., McEvoy L.M., Butcher E.C.
    Nature 363:461-464(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE (ISOFORM 1), INTERACTION WITH ALPHA-4/BETA-7 INTEGRIN, TISSUE SPECIFICITY.
    Strain: BALB/c.
    Tissue: Brain.
  2. "Organization, regulatory sequences, and alternatively spliced transcripts of the mucosal addressin cell adhesion molecule-1 (MAdCAM-1) gene."
    Sampaio S.O., Li X., Takeuchi M., Mei C., Francke U., Butcher E.C., Briskin M.J.
    J. Immunol. 155:2477-2486(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS 1 AND 2).
    Strain: SRB.
    Tissue: Liver.
  3. "An alternately spliced mRNA encoding functional domains of murine MAdCAM-1."
    Schiffer S.G., Day E., Latanision S.M., Tizard R., Osborn L.
    Biochem. Biophys. Res. Commun. 216:170-176(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), ALTERNATIVE SPLICING, FUNCTION.
    Strain: BALB/c.
    Tissue: Lymph node.
  4. "Analysis of expression of lymphocyte homing-related adhesion molecules in ALY mice deficient in lymph nodes and Peyer's patches."
    Koike R., Watanabe T., Satoh H., Hee C.S., Kitada K., Kuramoto T., Serikawa T., Miyawaki S., Miyasaka M.
    Cell. Immunol. 180:62-69(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1), DISEASE.
    Strain: BALB/c.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Colon.
  6. "Alpha 4 beta 7 integrin mediates lymphocyte binding to the mucosal vascular addressin MAdCAM-1."
    Berlin C., Berg E.L., Briskin M.J., Andrew D.P., Kilshaw P.J., Holzmann B., Weissman I.L., Hamann A., Butcher E.C.
    Cell 74:185-195(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ALPHA-4/BETA-7 INTEGRIN.
    Tissue: Lymph node.
  7. "A tissue-specific endothelial cell molecule involved in lymphocyte homing."
    Streeter P.R., Berg E.L., Rouse B.T.N., Bargatze R.F., Butcher E.C.
    Nature 331:41-46(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, FUNCTION.
  8. "Vascular addressins are induced on islet vessels during insulitis in nonobese diabetic mice and are involved in lymphoid cell binding to islet endothelium."
    Hanninen A., Taylor C., Streeter P.R., Stark L.S., Sarte J.M., Shizuru J.A., Simell O., Michie S.A.
    J. Clin. Invest. 92:2509-2515(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, INDUCTION.
    Strain: NOD.
  9. "L-selectin-mediated lymphocyte rolling on MAdCAM-1."
    Berg E.L., McEvoy L.M., Berlin C., Bargatze R.F., Butcher E.C.
    Nature 366:695-698(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH L-SELECTIN, GLYCOSYLATION.
  10. "Induction of the gene encoding mucosal vascular addressin cell adhesion molecule 1 by tumor necrosis factor alpha is mediated by NF-kappa B proteins."
    Takeuchi M., Baichwal V.R.
    Proc. Natl. Acad. Sci. U.S.A. 92:3561-3565(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY TNF-ALPHA.

Entry informationi

Entry nameiMADCA_MOUSE
AccessioniPrimary (citable) accession number: Q61826
Secondary accession number(s): O35530
, Q61278, Q64275, Q8R1M6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 2, 2004
Last sequence update: February 2, 2004
Last modified: October 29, 2014
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3