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Q61826 (MADCA_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 113. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mucosal addressin cell adhesion molecule 1

Short name=MAdCAM-1
Short name=mMAdCAM-1
Gene names
Name:Madcam1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length405 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Cell adhesion leukocyte receptor expressed by mucosal venules, helps to direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It can bind both the integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes. Both isoform 1 and isoform 2 can adhere to integrin alpha-4/beta-7. Isoform 2, lacking the mucin-like domain, may be specialized in supporting integrin alpha-4/beta-7-dependent adhesion strengthening, independent of L-selectin binding. Ref.3 Ref.7

Subunit structure

Homodimer Probable.

Subcellular location

Membrane; Single-pass type I membrane protein.

Tissue specificity

Highly expressed on high endothelial venules (HEV) of organized intestinal lymphoid tissues like the Peyer patches and mesenteric lymph nodes, and in the lamina propria of the intestine. Some expression found in the spleen, and low levels of expression in the peripheral lymph nodes and the lactating mammary gland. No expression was detected in the liver, kidneys, lungs or in normal brain. Expressed as well in brain endothelioma cells, and mucosal tissues which are in a chronic state of inflammation, such as inflammed pancreas. Ref.1 Ref.7 Ref.8

Induction

By TNF-alpha, and chronic inflammation. Ref.8 Ref.10

Post-translational modification

O-glycosylated; contains syalic acid. The Ser/Thr-rich mucin-like domain may provide possible sites for O-glycosylation. Ref.9

Involvement in disease

Absence of Madcam1 in the spleen has been found in aly/aly mice, but normal expression is found in intestinal venules. This aberrant expression is a secondary defect and not the direct cause of aly alymphoplasia, an autosomal recessive mutation which induces total aplasia of lymph nodes and Peyer patches. Ref.4

Sequence similarities

Contains 3 Ig-like (immunoglobulin-like) domains.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q61826-1)

Also known as: Long; MadCAM-384;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q61826-2)

Also known as: Short; MadCAM-240;

The sequence of this isoform differs from the canonical sequence as follows:
     219-363: VLQSQTSPKP...VPGQVTPNSS → A

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 Potential
Chain22 – 405384Mucosal addressin cell adhesion molecule 1
PRO_0000014854

Regions

Topological domain22 – 364343Extracellular Potential
Transmembrane365 – 38521Helical; Potential
Topological domain386 – 40520Cytoplasmic Potential
Domain22 – 10988Ig-like 1
Domain110 – 227118Ig-like 2
Domain258 – 357100Ig-like 3
Region221 – 25737Mucin-like

Amino acid modifications

Glycosylation2301N-linked (GlcNAc...) Potential
Glycosylation2531N-linked (GlcNAc...) Potential
Glycosylation3611N-linked (GlcNAc...) Potential
Disulfide bond45 ↔ 91 By similarity
Disulfide bond49 ↔ 95 By similarity
Disulfide bond132 ↔ 200 By similarity
Disulfide bond293 ↔ 341 Potential

Natural variations

Alternative sequence219 – 363145VLQSQ…TPNSS → A in isoform 2.
VSP_050423

Experimental info

Sequence conflict61A → S in BAA23364. Ref.4
Sequence conflict101A → S Ref.4
Sequence conflict121A → S Ref.4
Sequence conflict611L → R Ref.1
Sequence conflict611L → R Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (Long) (MadCAM-384) [UniParc].

Last modified February 2, 2004. Version 2.
Checksum: 4F2D08418EA51980

FASTA40543,652
        10         20         30         40         50         60 
MESILALLLA LALVPYQLSR GQSFQVNPPE SEVAVAMGTS LQITCSMSCD EGVARVHWRG 

        70         80         90        100        110        120 
LDTSLGSVQT LPGSSILSVR GMLSDTGTPV CVGSCGSRSF QHSVKILVYA FPDQLVVSPE 

       130        140        150        160        170        180 
FLVPGQDQVV SCTAHNIWPA DPNSLSFALL LGEQRLEGAQ ALEPEQEEEI QEAEGTPLFR 

       190        200        210        220        230        240 
MTQRWRLPSL GTPAPPALHC QVTMQLPKLV LTHRKEIPVL QSQTSPKPPN TTSAEPYILT 

       250        260        270        280        290        300 
SSSTAEAVST GLNITTLPSA PPYPKLSPRT LSSEGPCRPK IHQDLEAGWE LLCEASCGPG 

       310        320        330        340        350        360 
VTVRWTLAPG DLATYHKREA GAQAWLSVLP PGPMVEGWFQ CRQDPGGEVT NLYVPGQVTP 

       370        380        390        400 
NSSSTVVLWI GSLVLGLLAL VFLAYRLWKC YRPGPRPDTS SCTHL 

« Hide

Isoform 2 (Short) (MadCAM-240) [UniParc].

Checksum: BD1F0984799DF3B0
Show »

FASTA26128,357

References

« Hide 'large scale' references
[1]"MAdCAM-1 has homology to immunoglobulin and mucin-like adhesion receptors and to IgA1."
Briskin M.J., McEvoy L.M., Butcher E.C.
Nature 363:461-464(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE (ISOFORM 1), INTERACTION WITH ALPHA-4/BETA-7 INTEGRIN, TISSUE SPECIFICITY.
Strain: BALB/c.
Tissue: Brain.
[2]"Organization, regulatory sequences, and alternatively spliced transcripts of the mucosal addressin cell adhesion molecule-1 (MAdCAM-1) gene."
Sampaio S.O., Li X., Takeuchi M., Mei C., Francke U., Butcher E.C., Briskin M.J.
J. Immunol. 155:2477-2486(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS 1 AND 2).
Strain: SRB.
Tissue: Liver.
[3]"An alternately spliced mRNA encoding functional domains of murine MAdCAM-1."
Schiffer S.G., Day E., Latanision S.M., Tizard R., Osborn L.
Biochem. Biophys. Res. Commun. 216:170-176(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), ALTERNATIVE SPLICING, FUNCTION.
Strain: BALB/c.
Tissue: Lymph node.
[4]"Analysis of expression of lymphocyte homing-related adhesion molecules in ALY mice deficient in lymph nodes and Peyer's patches."
Koike R., Watanabe T., Satoh H., Hee C.S., Kitada K., Kuramoto T., Serikawa T., Miyawaki S., Miyasaka M.
Cell. Immunol. 180:62-69(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1), DISEASE.
Strain: BALB/c.
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Colon.
[6]"Alpha 4 beta 7 integrin mediates lymphocyte binding to the mucosal vascular addressin MAdCAM-1."
Berlin C., Berg E.L., Briskin M.J., Andrew D.P., Kilshaw P.J., Holzmann B., Weissman I.L., Hamann A., Butcher E.C.
Cell 74:185-195(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ALPHA-4/BETA-7 INTEGRIN.
Tissue: Lymph node.
[7]"A tissue-specific endothelial cell molecule involved in lymphocyte homing."
Streeter P.R., Berg E.L., Rouse B.T.N., Bargatze R.F., Butcher E.C.
Nature 331:41-46(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, FUNCTION.
[8]"Vascular addressins are induced on islet vessels during insulitis in nonobese diabetic mice and are involved in lymphoid cell binding to islet endothelium."
Hanninen A., Taylor C., Streeter P.R., Stark L.S., Sarte J.M., Shizuru J.A., Simell O., Michie S.A.
J. Clin. Invest. 92:2509-2515(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, INDUCTION.
Strain: NOD.
[9]"L-selectin-mediated lymphocyte rolling on MAdCAM-1."
Berg E.L., McEvoy L.M., Berlin C., Bargatze R.F., Butcher E.C.
Nature 366:695-698(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH L-SELECTIN, GLYCOSYLATION.
[10]"Induction of the gene encoding mucosal vascular addressin cell adhesion molecule 1 by tumor necrosis factor alpha is mediated by NF-kappa B proteins."
Takeuchi M., Baichwal V.R.
Proc. Natl. Acad. Sci. U.S.A. 92:3561-3565(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION BY TNF-ALPHA.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L21203 mRNA. Translation: AAA39485.1.
U14729, U14552 Genomic DNA. Translation: AAA81639.1.
U14729, U14552 Genomic DNA. Translation: AAA81638.1.
S80258 mRNA. Translation: AAB35609.1.
D50434 Genomic DNA. Translation: BAA23364.1.
BC024372 mRNA. Translation: AAH24372.1.
CCDSCCDS23981.1. [Q61826-1]
PIRI48645.
S33601.
RefSeqXP_006513356.1. XM_006513293.1. [Q61826-2]
UniGeneMm.391556.

3D structure databases

ProteinModelPortalQ61826.
SMRQ61826. Positions 22-220.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ61826.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID17123.
KEGGmmu:17123.

Organism-specific databases

CTD8174.
MGIMGI:103579. Madcam1.

Phylogenomic databases

eggNOGNOG26033.
HOGENOMHOG000060103.
HOVERGENHBG052394.
InParanoidQ61826.
KOK06779.
PhylomeDBQ61826.

Gene expression databases

GenevestigatorQ61826.

Family and domain databases

Gene3D2.60.40.10. 2 hits.
InterProIPR015169. Adhes-Ig-like.
IPR013783. Ig-like_fold.
[Graphical view]
PfamPF09085. Adhes-Ig_like. 1 hit.
[Graphical view]
ProDomPD336606. Adhes-Ig-like. 1 hit.
[Graphical view] [Entries sharing at least one domain]
ProtoNetSearch...

Other

PROQ61826.
SOURCESearch...

Entry information

Entry nameMADCA_MOUSE
AccessionPrimary (citable) accession number: Q61826
Secondary accession number(s): O35530 expand/collapse secondary AC list , Q61278, Q64275, Q8R1M6
Entry history
Integrated into UniProtKB/Swiss-Prot: February 2, 2004
Last sequence update: February 2, 2004
Last modified: July 9, 2014
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot