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Q61826

- MADCA_MOUSE

UniProt

Q61826 - MADCA_MOUSE

Protein

Mucosal addressin cell adhesion molecule 1

Gene

Madcam1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 114 (01 Oct 2014)
      Sequence version 2 (02 Feb 2004)
      Previous versions | rss
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    Functioni

    Cell adhesion leukocyte receptor expressed by mucosal venules, helps to direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It can bind both the integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes. Both isoform 1 and isoform 2 can adhere to integrin alpha-4/beta-7. Isoform 2, lacking the mucin-like domain, may be specialized in supporting integrin alpha-4/beta-7-dependent adhesion strengthening, independent of L-selectin binding.2 Publications

    GO - Biological processi

    1. cell adhesion Source: UniProtKB-KW
    2. keratinocyte differentiation Source: MGI
    3. leukocyte migration Source: MGI

    Keywords - Biological processi

    Cell adhesion

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mucosal addressin cell adhesion molecule 1
    Short name:
    MAdCAM-1
    Short name:
    mMAdCAM-1
    Gene namesi
    Name:Madcam1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Unplaced

    Organism-specific databases

    MGIiMGI:103579. Madcam1.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Involvement in diseasei

    Absence of Madcam1 in the spleen has been found in aly/aly mice, but normal expression is found in intestinal venules. This aberrant expression is a secondary defect and not the direct cause of aly alymphoplasia, an autosomal recessive mutation which induces total aplasia of lymph nodes and Peyer patches.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2121Sequence AnalysisAdd
    BLAST
    Chaini22 – 405384Mucosal addressin cell adhesion molecule 1PRO_0000014854Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi45 ↔ 91By similarity
    Disulfide bondi49 ↔ 95By similarity
    Disulfide bondi132 ↔ 200By similarity
    Glycosylationi230 – 2301N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi253 – 2531N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi293 ↔ 341Sequence Analysis
    Glycosylationi361 – 3611N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    O-glycosylated; contains syalic acid. The Ser/Thr-rich mucin-like domain may provide possible sites for O-glycosylation.1 Publication

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PRIDEiQ61826.

    Expressioni

    Tissue specificityi

    Highly expressed on high endothelial venules (HEV) of organized intestinal lymphoid tissues like the Peyer patches and mesenteric lymph nodes, and in the lamina propria of the intestine. Some expression found in the spleen, and low levels of expression in the peripheral lymph nodes and the lactating mammary gland. No expression was detected in the liver, kidneys, lungs or in normal brain. Expressed as well in brain endothelioma cells, and mucosal tissues which are in a chronic state of inflammation, such as inflammed pancreas.3 Publications

    Inductioni

    By TNF-alpha, and chronic inflammation.2 Publications

    Gene expression databases

    GenevestigatoriQ61826.

    Interactioni

    Subunit structurei

    Homodimer.Curated

    Structurei

    3D structure databases

    ProteinModelPortaliQ61826.
    SMRiQ61826. Positions 22-220.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini22 – 364343ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini386 – 40520CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei365 – 38521HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini22 – 10988Ig-like 1Add
    BLAST
    Domaini110 – 227118Ig-like 2Add
    BLAST
    Domaini258 – 357100Ig-like 3Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni221 – 25737Mucin-likeAdd
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG26033.
    HOGENOMiHOG000060103.
    HOVERGENiHBG052394.
    InParanoidiQ61826.
    KOiK06779.
    PhylomeDBiQ61826.

    Family and domain databases

    Gene3Di2.60.40.10. 2 hits.
    InterProiIPR015169. Adhes-Ig-like.
    IPR013783. Ig-like_fold.
    [Graphical view]
    PfamiPF09085. Adhes-Ig_like. 1 hit.
    [Graphical view]
    ProDomiPD336606. Adhes-Ig-like. 1 hit.
    [Graphical view] [Entries sharing at least one domain]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q61826-1) [UniParc]FASTAAdd to Basket

    Also known as: Long, MadCAM-384

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MESILALLLA LALVPYQLSR GQSFQVNPPE SEVAVAMGTS LQITCSMSCD    50
    EGVARVHWRG LDTSLGSVQT LPGSSILSVR GMLSDTGTPV CVGSCGSRSF 100
    QHSVKILVYA FPDQLVVSPE FLVPGQDQVV SCTAHNIWPA DPNSLSFALL 150
    LGEQRLEGAQ ALEPEQEEEI QEAEGTPLFR MTQRWRLPSL GTPAPPALHC 200
    QVTMQLPKLV LTHRKEIPVL QSQTSPKPPN TTSAEPYILT SSSTAEAVST 250
    GLNITTLPSA PPYPKLSPRT LSSEGPCRPK IHQDLEAGWE LLCEASCGPG 300
    VTVRWTLAPG DLATYHKREA GAQAWLSVLP PGPMVEGWFQ CRQDPGGEVT 350
    NLYVPGQVTP NSSSTVVLWI GSLVLGLLAL VFLAYRLWKC YRPGPRPDTS 400
    SCTHL 405
    Length:405
    Mass (Da):43,652
    Last modified:February 2, 2004 - v2
    Checksum:i4F2D08418EA51980
    GO
    Isoform 2 (identifier: Q61826-2) [UniParc]FASTAAdd to Basket

    Also known as: Short, MadCAM-240

    The sequence of this isoform differs from the canonical sequence as follows:
         219-363: VLQSQTSPKP...VPGQVTPNSS → A

    Show »
    Length:261
    Mass (Da):28,357
    Checksum:iBD1F0984799DF3B0
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti6 – 61A → S in BAA23364. (PubMed:9316640)Curated
    Sequence conflicti10 – 101A → S(PubMed:9316640)Curated
    Sequence conflicti12 – 121A → S(PubMed:9316640)Curated
    Sequence conflicti61 – 611L → R(PubMed:8502297)Curated
    Sequence conflicti61 – 611L → R(PubMed:9316640)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei219 – 363145VLQSQ…TPNSS → A in isoform 2. 2 PublicationsVSP_050423Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L21203 mRNA. Translation: AAA39485.1.
    U14729, U14552 Genomic DNA. Translation: AAA81639.1.
    U14729, U14552 Genomic DNA. Translation: AAA81638.1.
    S80258 mRNA. Translation: AAB35609.1.
    D50434 Genomic DNA. Translation: BAA23364.1.
    BC024372 mRNA. Translation: AAH24372.1.
    CCDSiCCDS23981.1. [Q61826-1]
    PIRiI48645.
    S33601.
    RefSeqiXP_006513356.1. XM_006513293.1. [Q61826-2]
    UniGeneiMm.391556.

    Genome annotation databases

    GeneIDi17123.
    KEGGimmu:17123.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L21203 mRNA. Translation: AAA39485.1 .
    U14729 , U14552 Genomic DNA. Translation: AAA81639.1 .
    U14729 , U14552 Genomic DNA. Translation: AAA81638.1 .
    S80258 mRNA. Translation: AAB35609.1 .
    D50434 Genomic DNA. Translation: BAA23364.1 .
    BC024372 mRNA. Translation: AAH24372.1 .
    CCDSi CCDS23981.1. [Q61826-1 ]
    PIRi I48645.
    S33601.
    RefSeqi XP_006513356.1. XM_006513293.1. [Q61826-2 ]
    UniGenei Mm.391556.

    3D structure databases

    ProteinModelPortali Q61826.
    SMRi Q61826. Positions 22-220.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi Q61826.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 17123.
    KEGGi mmu:17123.

    Organism-specific databases

    CTDi 8174.
    MGIi MGI:103579. Madcam1.

    Phylogenomic databases

    eggNOGi NOG26033.
    HOGENOMi HOG000060103.
    HOVERGENi HBG052394.
    InParanoidi Q61826.
    KOi K06779.
    PhylomeDBi Q61826.

    Miscellaneous databases

    PROi Q61826.
    SOURCEi Search...

    Gene expression databases

    Genevestigatori Q61826.

    Family and domain databases

    Gene3Di 2.60.40.10. 2 hits.
    InterProi IPR015169. Adhes-Ig-like.
    IPR013783. Ig-like_fold.
    [Graphical view ]
    Pfami PF09085. Adhes-Ig_like. 1 hit.
    [Graphical view ]
    ProDomi PD336606. Adhes-Ig-like. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    ProtoNeti Search...

    Publicationsi

    1. "MAdCAM-1 has homology to immunoglobulin and mucin-like adhesion receptors and to IgA1."
      Briskin M.J., McEvoy L.M., Butcher E.C.
      Nature 363:461-464(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE (ISOFORM 1), INTERACTION WITH ALPHA-4/BETA-7 INTEGRIN, TISSUE SPECIFICITY.
      Strain: BALB/c.
      Tissue: Brain.
    2. "Organization, regulatory sequences, and alternatively spliced transcripts of the mucosal addressin cell adhesion molecule-1 (MAdCAM-1) gene."
      Sampaio S.O., Li X., Takeuchi M., Mei C., Francke U., Butcher E.C., Briskin M.J.
      J. Immunol. 155:2477-2486(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS 1 AND 2).
      Strain: SRB.
      Tissue: Liver.
    3. "An alternately spliced mRNA encoding functional domains of murine MAdCAM-1."
      Schiffer S.G., Day E., Latanision S.M., Tizard R., Osborn L.
      Biochem. Biophys. Res. Commun. 216:170-176(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), ALTERNATIVE SPLICING, FUNCTION.
      Strain: BALB/c.
      Tissue: Lymph node.
    4. "Analysis of expression of lymphocyte homing-related adhesion molecules in ALY mice deficient in lymph nodes and Peyer's patches."
      Koike R., Watanabe T., Satoh H., Hee C.S., Kitada K., Kuramoto T., Serikawa T., Miyawaki S., Miyasaka M.
      Cell. Immunol. 180:62-69(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1), DISEASE.
      Strain: BALB/c.
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Colon.
    6. "Alpha 4 beta 7 integrin mediates lymphocyte binding to the mucosal vascular addressin MAdCAM-1."
      Berlin C., Berg E.L., Briskin M.J., Andrew D.P., Kilshaw P.J., Holzmann B., Weissman I.L., Hamann A., Butcher E.C.
      Cell 74:185-195(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ALPHA-4/BETA-7 INTEGRIN.
      Tissue: Lymph node.
    7. "A tissue-specific endothelial cell molecule involved in lymphocyte homing."
      Streeter P.R., Berg E.L., Rouse B.T.N., Bargatze R.F., Butcher E.C.
      Nature 331:41-46(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, FUNCTION.
    8. "Vascular addressins are induced on islet vessels during insulitis in nonobese diabetic mice and are involved in lymphoid cell binding to islet endothelium."
      Hanninen A., Taylor C., Streeter P.R., Stark L.S., Sarte J.M., Shizuru J.A., Simell O., Michie S.A.
      J. Clin. Invest. 92:2509-2515(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, INDUCTION.
      Strain: NOD.
    9. "L-selectin-mediated lymphocyte rolling on MAdCAM-1."
      Berg E.L., McEvoy L.M., Berlin C., Bargatze R.F., Butcher E.C.
      Nature 366:695-698(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH L-SELECTIN, GLYCOSYLATION.
    10. "Induction of the gene encoding mucosal vascular addressin cell adhesion molecule 1 by tumor necrosis factor alpha is mediated by NF-kappa B proteins."
      Takeuchi M., Baichwal V.R.
      Proc. Natl. Acad. Sci. U.S.A. 92:3561-3565(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION BY TNF-ALPHA.

    Entry informationi

    Entry nameiMADCA_MOUSE
    AccessioniPrimary (citable) accession number: Q61826
    Secondary accession number(s): O35530
    , Q61278, Q64275, Q8R1M6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 2, 2004
    Last sequence update: February 2, 2004
    Last modified: October 1, 2014
    This is version 114 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3