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Protein

Mucosal addressin cell adhesion molecule 1

Gene

Madcam1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cell adhesion leukocyte receptor expressed by mucosal venules, helps to direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It can bind both the integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes. Both isoform 1 and isoform 2 can adhere to integrin alpha-4/beta-7. Isoform 2, lacking the mucin-like domain, may be specialized in supporting integrin alpha-4/beta-7-dependent adhesion strengthening, independent of L-selectin binding.2 Publications

GO - Molecular functioni

GO - Biological processi

  • heterotypic cell-cell adhesion Source: GO_Central
  • integrin-mediated signaling pathway Source: GO_Central
  • keratinocyte differentiation Source: MGI
  • leukocyte migration Source: MGI
  • leukocyte tethering or rolling Source: GO_Central
  • positive regulation of leukocyte migration Source: GO_Central
  • positive regulation of lymphocyte migration Source: InterPro

Keywordsi

Biological processCell adhesion

Enzyme and pathway databases

ReactomeiR-MMU-198933 Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell
R-MMU-216083 Integrin cell surface interactions

Names & Taxonomyi

Protein namesi
Recommended name:
Mucosal addressin cell adhesion molecule 1
Short name:
MAdCAM-1
Short name:
mMAdCAM-1
Gene namesi
Name:Madcam1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:103579 Madcam1

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini22 – 364ExtracellularSequence analysisAdd BLAST343
Transmembranei365 – 385HelicalSequence analysisAdd BLAST21
Topological domaini386 – 405CytoplasmicSequence analysisAdd BLAST20

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Involvement in diseasei

Absence of Madcam1 in the spleen has been found in aly/aly mice, but normal expression is found in intestinal venules. This aberrant expression is a secondary defect and not the direct cause of aly alymphoplasia, an autosomal recessive mutation which induces total aplasia of lymph nodes and Peyer patches.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_000001485422 – 405Mucosal addressin cell adhesion molecule 1Add BLAST384

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi45 ↔ 91By similarity
Disulfide bondi49 ↔ 95By similarity
Disulfide bondi132 ↔ 200By similarity
Glycosylationi230N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi253N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi293 ↔ 341Sequence analysis
Glycosylationi361N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

O-glycosylated; contains syalic acid. The Ser/Thr-rich mucin-like domain may provide possible sites for O-glycosylation.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ61826
PRIDEiQ61826

PTM databases

PhosphoSitePlusiQ61826

Expressioni

Tissue specificityi

Highly expressed on high endothelial venules (HEV) of organized intestinal lymphoid tissues like the Peyer patches and mesenteric lymph nodes, and in the lamina propria of the intestine. Some expression found in the spleen, and low levels of expression in the peripheral lymph nodes and the lactating mammary gland. No expression was detected in the liver, kidneys, lungs or in normal brain. Expressed as well in brain endothelioma cells, and mucosal tissues which are in a chronic state of inflammation, such as inflammed pancreas.3 Publications

Inductioni

By TNF-alpha, and chronic inflammation.2 Publications

Gene expression databases

ExpressionAtlasiQ61826 baseline and differential

Interactioni

Subunit structurei

Homodimer.Curated

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000020554

Structurei

3D structure databases

ProteinModelPortaliQ61826
SMRiQ61826
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini22 – 109Ig-like 1Add BLAST88
Domaini110 – 227Ig-like 2Add BLAST118
Domaini258 – 357Ig-like 3Add BLAST100

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni221 – 257Mucin-likeAdd BLAST37

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410J133 Eukaryota
ENOG411140D LUCA
GeneTreeiENSGT00510000049549
HOGENOMiHOG000060103
HOVERGENiHBG052394
InParanoidiQ61826
PhylomeDBiQ61826

Family and domain databases

Gene3Di2.60.40.10, 2 hits
InterProiView protein in InterPro
IPR015169 Adhes-Ig-like
IPR036179 Ig-like_dom_sf
IPR013783 Ig-like_fold
IPR037413 MADCAM1
PANTHERiPTHR14162 PTHR14162, 1 hit
PfamiView protein in Pfam
PF09085 Adhes-Ig_like, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD336606 Adhes-Ig-like, 1 hit
SUPFAMiSSF48726 SSF48726, 2 hits

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q61826-1) [UniParc]FASTAAdd to basket
Also known as: Long, MadCAM-384

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MESILALLLA LALVPYQLSR GQSFQVNPPE SEVAVAMGTS LQITCSMSCD
60 70 80 90 100
EGVARVHWRG LDTSLGSVQT LPGSSILSVR GMLSDTGTPV CVGSCGSRSF
110 120 130 140 150
QHSVKILVYA FPDQLVVSPE FLVPGQDQVV SCTAHNIWPA DPNSLSFALL
160 170 180 190 200
LGEQRLEGAQ ALEPEQEEEI QEAEGTPLFR MTQRWRLPSL GTPAPPALHC
210 220 230 240 250
QVTMQLPKLV LTHRKEIPVL QSQTSPKPPN TTSAEPYILT SSSTAEAVST
260 270 280 290 300
GLNITTLPSA PPYPKLSPRT LSSEGPCRPK IHQDLEAGWE LLCEASCGPG
310 320 330 340 350
VTVRWTLAPG DLATYHKREA GAQAWLSVLP PGPMVEGWFQ CRQDPGGEVT
360 370 380 390 400
NLYVPGQVTP NSSSTVVLWI GSLVLGLLAL VFLAYRLWKC YRPGPRPDTS

SCTHL
Length:405
Mass (Da):43,652
Last modified:February 2, 2004 - v2
Checksum:i4F2D08418EA51980
GO
Isoform 2 (identifier: Q61826-2) [UniParc]FASTAAdd to basket
Also known as: Short, MadCAM-240

The sequence of this isoform differs from the canonical sequence as follows:
     219-363: VLQSQTSPKP...VPGQVTPNSS → A

Show »
Length:261
Mass (Da):28,357
Checksum:iBD1F0984799DF3B0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti6A → S in BAA23364 (PubMed:9316640).Curated1
Sequence conflicti10A → S (PubMed:9316640).Curated1
Sequence conflicti12A → S (PubMed:9316640).Curated1
Sequence conflicti61L → R (PubMed:8502297).Curated1
Sequence conflicti61L → R (PubMed:9316640).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_050423219 – 363VLQSQ…TPNSS → A in isoform 2. 2 PublicationsAdd BLAST145

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L21203 mRNA Translation: AAA39485.1
U14729, U14552 Genomic DNA Translation: AAA81639.1
U14729, U14552 Genomic DNA Translation: AAA81638.1
S80258 mRNA Translation: AAB35609.1
D50434 Genomic DNA Translation: BAA23364.1
BC024372 mRNA Translation: AAH24372.1
CCDSiCCDS23981.1 [Q61826-1]
PIRiI48645
S33601
RefSeqiXP_006513356.1, XM_006513293.3
UniGeneiMm.391556

Genome annotation databases

EnsembliENSMUST00000217748; ENSMUSP00000151928; ENSMUSG00000020310 [Q61826-2]
GeneIDi17123

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiMADCA_MOUSE
AccessioniPrimary (citable) accession number: Q61826
Secondary accession number(s): O35530
, Q61278, Q64275, Q8R1M6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 2, 2004
Last sequence update: February 2, 2004
Last modified: April 25, 2018
This is version 140 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health