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Q61817

- CREB3_MOUSE

UniProt

Q61817 - CREB3_MOUSE

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Protein
Cyclic AMP-responsive element-binding protein 3
Gene
Creb3, Lzip
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Endoplasmic reticulum (ER)-bound transcription factor that plays a role in the unfolded protein response (UPR). Involved in cell proliferation and migration, tumor suppression and inflammatory gene expression. Plays a role in the unfolded protein response (UPR). Acts as a positive regulator of LKN-1/CCL15-induced chemotaxis signaling of leukocyte cell migration. Functions as a negative transcriptional regulator in ligand-induced transcriptional activation of the glucocorticoid receptor NR3C1 by recruiting and activating histone deacetylases (HDAC1, HDAC2 and HDAC6). Decreases the acetylation level of histone H4. Does not promote the chemotactic activity of leukocyte cells By similarity.
Processed cyclic AMP-responsive element-binding protein 3: acts as a transcription factor that activates unfolded protein response (UPR) target genes during endoplasmic reticulum (ER) stress response. Promotes cell survival against ER stress-induced apoptotic cell death during UPR. Activates transcription from CRE and C/EBP-containing reporter genes. Induces transcriptional activation of chemokine receptors. It's transcriptional activity is inhibited by CREBZF in a HCFC1-dependent manner. Binds DNA to the cAMP response element (CRE) (consensus: 5'-GTGACGT[AG][AG]-3') and C/EBP sequences present in many cellular promoters. Binds to the unfolded protein respons element (UPRE) consensus sequences sites. Binds DNA to the 5'-CCAC[GA]-3'half of ERSE II (5'-ATTGG-N-CCACG-3'). Associates with chromatin to the HERPUD1 promoter By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei301 – 3022Cleavage; by PS1 By similarity

GO - Molecular functioni

  1. RNA polymerase II regulatory region sequence-specific DNA binding Source: UniProtKB
  2. chromatin binding Source: UniProtKB
  3. protein homodimerization activity Source: UniProtKB
  4. sequence-specific DNA binding Source: MGI
  5. sequence-specific DNA binding transcription factor activity Source: UniProtKB

GO - Biological processi

  1. chemotaxis Source: UniProtKB-KW
  2. cytoplasmic sequestering of transcription factor Source: UniProtKB
  3. establishment of viral latency Source: UniProtKB
  4. induction of positive chemotaxis Source: UniProtKB
  5. negative regulation of ligand-dependent nuclear receptor transcription coactivator activity Source: UniProtKB
  6. positive regulation of deacetylase activity Source: UniProtKB
  7. positive regulation of defense response to virus by host Source: UniProtKB
  8. positive regulation of monocyte chemotaxis Source: UniProtKB
  9. positive regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  10. positive regulation of transcription from RNA polymerase II promoter involved in unfolded protein response Source: UniProtKB
  11. regulation of cell growth Source: UniProtKB
  12. release from viral latency Source: UniProtKB
  13. response to endoplasmic reticulum stress Source: UniProtKB
  14. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

Chemotaxis, Transcription, Transcription regulation, Unfolded protein response

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclic AMP-responsive element-binding protein 3
Short name:
CREB-3
Short name:
cAMP-responsive element-binding protein 3
Alternative name(s):
Transcription factor LZIP
Cleaved into the following chain:
Gene namesi
Name:Creb3
Synonyms:Lzip
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Unplaced

Organism-specific databases

MGIiMGI:99946. Creb3.

Subcellular locationi

Endoplasmic reticulum membrane; Single-pass type II membrane protein By similarity. Membrane By similarity. Nucleus By similarity. Cytoplasm By similarity
Note: Colocalizes with TM7SF4 in the ER membrane of immature dendritic cell (DC). Colocalizes with CANX, CCR1, HCFC1 in the ER membrane. Colocalizes with HCFC1 in neuronal cell bodies of the trigeminal ganglia By similarity.
Chain Processed cyclic AMP-responsive element-binding protein 3 : Nucleus By similarity
Note: Upon RIP activation the transcriptional active processed cyclic AMP-responsive element-binding protein 3 form translocates into the nucleus. Detected in the nucleus upon dendritic cell maturation and RIP activation. Colocalizes with CREBRF in nuclear foci. Colocalizes with CREBZF in promyelocytic leukemia protein nuclear bodies (PML-NB) By similarity.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 261261Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei262 – 28221Helical; Signal-anchor for type II membrane protein; Reviewed prediction
Add
BLAST
Topological domaini283 – 404122Lumenal Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. Golgi membrane Source: UniProtKB
  2. cytoplasm Source: UniProtKB
  3. endoplasmic reticulum membrane Source: UniProtKB
  4. integral component of endoplasmic reticulum membrane Source: UniProtKB
  5. membrane Source: UniProtKB
  6. neuronal cell body Source: UniProtKB
  7. nuclear body Source: UniProtKB
  8. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 404404Cyclic AMP-responsive element-binding protein 3
PRO_0000076603Add
BLAST
Chaini1 – ?Processed cyclic AMP-responsive element-binding protein 3PRO_0000296205

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi342 – 3421N-linked (GlcNAc...) Reviewed prediction
Glycosylationi380 – 3801N-linked (GlcNAc...) Reviewed prediction

Post-translational modificationi

The ER membrane embedded cyclic AMP-responsive element-binding protein 3 form is first proteolytically cleaved by site-1 protease (S1P) that generates membrane-associated N-terminus and a luminal C-terminus forms. The membrane-associated N-terminus form is further proteolytically processed probably by the site-2 protease (S2P) through a regulated intramembrane proteolysis (RIP), releasing the transcriptional active processed cyclic AMP-responsive element-binding protein 3 form, which is transported to the nucleus. The proteolytic cleavage is strongly induced during dendritic cell (DC) maturation and inhibited by TM7SF4 By similarity.
The processed cyclic AMP-responsive element-binding protein 3 is rapidly degraded By similarity.
N-glycosylated By similarity.

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiQ61817.

PTM databases

PhosphoSiteiQ61817.

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Gene expression databases

CleanExiMM_CREB3.
GenevestigatoriQ61817.

Interactioni

Subunit structurei

Homodimer. Interacts with HCFC1; the interaction is required to stimulate CREB3 transcriptional activity. Interacts with CREBZF; the interaction occurs only in combination with HCFC1. Interacts (via central part and transmembrane region) with TM7SF4 (via C-terminus cytoplasmic domain). Interacts with OS9. Interacts (via leucine-zipper domain) with CREBRF (via leucine-zipper domain); the interaction occurs only after CREB3 activation and promotes CREB3 degradation. Interacts (via C-terminal domain) with CCR1 By similarity.

Protein-protein interaction databases

IntActiQ61817. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ61817.
SMRiQ61817. Positions 157-242.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini185 – 24864bZIP
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni187 – 22539Basic motif By similarity
Add
BLAST
Regioni227 – 24822Leucine-zipper By similarity
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi19 – 235LXXLL motif 1
Motifi64 – 685LXXLL motif 2
Motifi87 – 904HCFC1-binding-motif (HBM)

Sequence similaritiesi

Belongs to the bZIP family. ATF subfamily.

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG254445.
HOGENOMiHOG000133026.
HOVERGENiHBG051114.
PhylomeDBiQ61817.

Family and domain databases

InterProiIPR004827. bZIP.
[Graphical view]
PfamiPF00170. bZIP_1. 1 hit.
[Graphical view]
SMARTiSM00338. BRLZ. 1 hit.
[Graphical view]
PROSITEiPS50217. BZIP. 1 hit.
PS00036. BZIP_BASIC. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q61817-1) [UniParc]FASTAAdd to Basket

Also known as: LZIP-1

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MDPGGQDLLA LDPGDQDLLG FLLEESGDLW AATEPDVKAS LDLELSPSEN    50
SVQELSDWEV EDLLSSLLSP SVSRDVLGSS SSSILHDHNY SLPQEHVSID 100
LGECEMISCR GRRELTGLAG STFPFADTES FEKEGFHVTP LPGEERAAEQ 150
EMSRLILTEE EKKLLEKEGL TLPSTLPLTK VEEQVLKRVR RKIRNKRAAQ 200
ESRKKKKVYV VGLESRVLKY TAQNRELQNK VQRLEEQNLS LLDQLRKLQA 250
MVIEIANKTS SGSTCVLVLV FSFCLLLVPA MYSSDARGSV PAEYVVLHRK 300
LRALPSEDDH QPKPSALSSE LPMDSTHQSL DSSEHMFLVS SNFSCVLYHA 350
PQAEQPLHWP LWDLSSEMLF SDSNLLLQAN LSESEGWQPN HSPSLVIFQG 400
RYSG 404
Length:404
Mass (Da):45,112
Last modified:November 9, 2004 - v2
Checksum:i96E4124256D4BD44
GO
Isoform 2 (identifier: Q61817-2) [UniParc]FASTAAdd to Basket

Also known as: LZIP-2

The sequence of this isoform differs from the canonical sequence as follows:
     102-126: Missing.

Show »
Length:379
Mass (Da):42,441
Checksum:i442BC9840B919997
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei102 – 12625Missing in isoform 2.
VSP_011839Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti263 – 2631S → T in AAC37645. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L22167 Unassigned DNA. Translation: AAC37645.1.
BC002094 mRNA. Translation: AAH02094.1.
AK007665 mRNA. Translation: BAB25173.1.
CCDSiCCDS18102.1. [Q61817-2]
UniGeneiMm.12407.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L22167 Unassigned DNA. Translation: AAC37645.1 .
BC002094 mRNA. Translation: AAH02094.1 .
AK007665 mRNA. Translation: BAB25173.1 .
CCDSi CCDS18102.1. [Q61817-2 ]
UniGenei Mm.12407.

3D structure databases

ProteinModelPortali Q61817.
SMRi Q61817. Positions 157-242.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q61817. 1 interaction.

PTM databases

PhosphoSitei Q61817.

Proteomic databases

PRIDEi Q61817.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Organism-specific databases

MGIi MGI:99946. Creb3.

Phylogenomic databases

eggNOGi NOG254445.
HOGENOMi HOG000133026.
HOVERGENi HBG051114.
PhylomeDBi Q61817.

Miscellaneous databases

PROi Q61817.
SOURCEi Search...

Gene expression databases

CleanExi MM_CREB3.
Genevestigatori Q61817.

Family and domain databases

InterProi IPR004827. bZIP.
[Graphical view ]
Pfami PF00170. bZIP_1. 1 hit.
[Graphical view ]
SMARTi SM00338. BRLZ. 1 hit.
[Graphical view ]
PROSITEi PS50217. BZIP. 1 hit.
PS00036. BZIP_BASIC. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "LZIP-1 and LZIP-2: two novel members of the bZIP family."
    Burbelo P.D., Gabriel G.C., Kibbey M.C., Yamada Y., Kleinman H.K., Weeks B.S.
    Gene 139:241-245(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE (ISOFORMS 1 AND 2), TISSUE SPECIFICITY.
    Strain: BALB/c.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: FVB/N.
    Tissue: Mammary tumor.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 157-404.
    Strain: C57BL/6J.
    Tissue: Pancreas.

Entry informationi

Entry nameiCREB3_MOUSE
AccessioniPrimary (citable) accession number: Q61817
Secondary accession number(s): Q99M21, Q9CVK9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 9, 2004
Last sequence update: November 9, 2004
Last modified: July 9, 2014
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi