Reviewed, UniProtKB/Swiss-Prot Q61810 (LTBP3_MOUSE)

Names and origin

Protein names

Recommended name:
    Latent-transforming growth factor beta-binding protein 3
      Short name=LTBP-3

Gene names

Name:

Ltbp3

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	1268 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May be involved in the assembly, secretion and targeting of TGF- beta1 to sites at which it is stored and/or activated. May play critical roles in controlling and directing the activity of TGF-beta1. May have a structural role in the extra cellular matrix (ECM).
Subunit structure	Forms part of the large latent transforming growth factor beta (TGFB1) precursor complex; removal is essential for activation of complex.
Subcellular location	Secreted. Note: Secretion occurs after coexpression with TGFB1 and requires complexing with 'Cys-33' of the TGFB1 propeptide.
Developmental stage	At 8.5-9.0 dpc highly expressed in liver. Significant expression was also seen in the developing central nervous, somites and cardiovascular tissue. At 13.5-16.5 dpc expression was seen in osteoblasts, respiratory epithelial cells, and nephrons and dermal connective tissue.
Domain	Associates covalently with small latent TGF-beta complex via Repeat C.
Post-translational modification	Contains hydroxylated asparagine residues By similarity.
Sequence similarities	Belongs to the LTBP family. Contains 13 EGF-like domains. Contains 3 TB (TGF-beta binding) domains.
Sequence caution	The sequence AAB53015.1 differs from that shown. Reason: Frameshift at positions 6, 38, 56, 155, 158, 160, 480 and 775.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Alternative splicing
Domain	EGF-like domain Repeat Signal
Ligand	Growth factor binding
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological process	skeletal system development Inferred from mutant phenotype. Source: MGI transforming growth factor beta receptor signaling pathway Ref.1 Inferred from mutant phenotype. Source: MGI
Cellular component	extracellular region Ref.1 Inferred from direct assay. Source: MGI
Molecular function	calcium ion binding Inferred from electronic annotation. Source: InterPro growth factor binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q61810-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q61810-2)

The sequence of this isoform differs from the canonical sequence as follows:
     860-882: KKDIDECSQDPGLCLPHACENLQ → QGMRSWPGILKGEAGQCDLFDTL
     883-1268: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 38	38	Potential
	Chain	39 – 1268	1230	Latent-transforming growth factor beta-binding protein 3		PRO_0000007647
Regions
	Domain	106 – 138	33	EGF-like 1
	Domain	274 – 328	55	TB 1
	Domain	352 – 392	41	EGF-like 2; calcium-binding Potential
	Domain	400 – 452	53	TB 2
	Domain	570 – 611	42	EGF-like 3
	Domain	612 – 655	44	EGF-like 4; calcium-binding Potential
	Domain	656 – 698	43	EGF-like 5; calcium-binding Potential
	Domain	740 – 779	40	EGF-like 6; calcium-binding Potential
	Domain	780 – 820	41	EGF-like 7; calcium-binding Potential
	Domain	821 – 860	40	EGF-like 8; calcium-binding Potential
	Domain	862 – 903	42	EGF-like 9; calcium-binding Potential
	Domain	912 – 966	55	TB 3
	Domain	988 – 1030	43	EGF-like 10; calcium-binding Potential
	Domain	1031 – 1070	40	EGF-like 11; calcium-binding Potential
	Domain	1201 – 1246	46	EGF-like 12; calcium-binding Potential
	Compositional bias	21 – 32	12	Poly-Leu
	Compositional bias	47 – 52	6	Poly-Gly
	Compositional bias	191 – 292	102	Pro-rich
	Compositional bias	480 – 556	77	Pro-rich
	Compositional bias	574 – 889	316	Cys-rich
Amino acid modifications
	Glycosylation	86	1	N-linked (GlcNAc...) Potential
	Glycosylation	346	1	N-linked (GlcNAc...) Potential
	Glycosylation	840	1	N-linked (GlcNAc...) Potential
	Glycosylation	931	1	N-linked (GlcNAc...) Potential
	Glycosylation	1222	1	N-linked (GlcNAc...) Potential
	Disulfide bond	110 ↔ 120		By similarity
	Disulfide bond	114 ↔ 126		By similarity
	Disulfide bond	128 ↔ 137		By similarity
	Disulfide bond	356 ↔ 367		By similarity
	Disulfide bond	363 ↔ 376		By similarity
	Disulfide bond	378 ↔ 391		By similarity
	Disulfide bond	574 ↔ 586		By similarity
	Disulfide bond	581 ↔ 595		By similarity
	Disulfide bond	597 ↔ 610		By similarity
	Disulfide bond	616 ↔ 628		By similarity
	Disulfide bond	621 ↔ 637		By similarity
	Disulfide bond	639 ↔ 654		By similarity
	Disulfide bond	660 ↔ 672		By similarity
	Disulfide bond	666 ↔ 681		By similarity
	Disulfide bond	683 ↔ 697		By similarity
	Disulfide bond	744 ↔ 755		By similarity
	Disulfide bond	750 ↔ 764		By similarity
	Disulfide bond	766 ↔ 778		By similarity
	Disulfide bond	784 ↔ 795		By similarity
	Disulfide bond	790 ↔ 804		By similarity
	Disulfide bond	806 ↔ 819		By similarity
	Disulfide bond	825 ↔ 836		By similarity
	Disulfide bond	831 ↔ 845		By similarity
	Disulfide bond	847 ↔ 859		By similarity
	Disulfide bond	866 ↔ 878		By similarity
	Disulfide bond	873 ↔ 887		By similarity
	Disulfide bond	889 ↔ 902		By similarity
	Disulfide bond	992 ↔ 1005		By similarity
	Disulfide bond	1000 ↔ 1014		By similarity
	Disulfide bond	1016 ↔ 1029		By similarity
	Disulfide bond	1035 ↔ 1046		By similarity
	Disulfide bond	1041 ↔ 1055		By similarity
	Disulfide bond	1057 ↔ 1070		By similarity
	Disulfide bond	1205 ↔ 1220		By similarity
	Disulfide bond	1215 ↔ 1229		By similarity
	Disulfide bond	1231 ↔ 1245		By similarity
Natural variations
	Alternative sequence	860 – 882	23	KKDID…CENLQ → QGMRSWPGILKGEAGQCDLF DTL in isoform 2.		VSP_009242
	Alternative sequence	883 – 1268	386	Missing in isoform 2.		VSP_009243
Experimental info
	Sequence conflict	16	1	H → R in AAB53015. Ref.1
	Sequence conflict	20	1	A → G in AAB53015. Ref.1
	Sequence conflict	35	1	Missing in AAB53015. Ref.1
	Sequence conflict	45	1	E → Q in AAB53015. Ref.1
	Sequence conflict	104	1	A → G in BAC38053. Ref.2
	Sequence conflict	172	1	E → Q in BAC38053. Ref.2
	Sequence conflict	263	1	P → Q in BAC38053. Ref.2
	Sequence conflict	328	1	V → G in BAC38053. Ref.2
	Sequence conflict	361 – 363	3	NVC → MCR in BAC38053. Ref.2
	Sequence conflict	387 – 389	3	LAA → SRT in BAC38053. Ref.2
	Sequence conflict	453	1	P → PA in BAC38053. Ref.2
	Sequence conflict	661	1	A → T in BAC38053. Ref.2
	Sequence conflict	768	1	Q → QG in BAC38053. Ref.2
	Sequence conflict	849	1	L → Q in BAC38053. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 16, 2004. Version 3. Checksum: 98F632F249ACB4DD	FASTA	1,268	135,702
	10 20 30 40 50 60 MPGPRGAAHG LAPAMHQAGA LGLLALLLLA LLGPGGGAEG GPAGERGTGG GGALARERFK 70 80 90 100 110 120 VVFAPVICKR TCLKGQCRDS CQQGSNMTLI GENGHSTDTL TGSAFRVVVC PLPCMNGGQC 130 140 150 160 170 180 SSRNQCLCPP DFTGRFCQVP AAGTGAGTGS SGPGLARTGA MSTGPLPPLA PEGESVASKH 190 200 210 220 230 240 AIYAVQVIAD PPGPGEGPPA QHAAFLVPLG PGQISAEVQA PPPVVNVRVH HPPEASVQVH 250 260 270 280 290 300 RIEGPNAEGP ASSQHLLPHP KPPHPRPPTQ KPLGRCFQDT LPKQPCGSNP LPGLTKQEDC 310 320 330 340 350 360 CGSIGTAWGQ SKCHKCPQLQ YTGVQKPVPV RGEVGADCPQ GYKRLNSTHC QDINECAMPG 370 380 390 400 410 420 NVCHGDCLNN PGSYRCVCPP GHSLGPLAAQ CIADKPEEKS LCFRLVSTEH QCQHPLTTRL 430 440 450 460 470 480 TRQLCCCSVG KAWGARCQRC PADGTAAFKE ICPGKGYHIL TSHQTLTIQG ESDFSLFLHP 490 500 510 520 530 540 DGPPKPQQLP ESPSRAPPLE DTEEERGVTM DPPVSEERSV QQSHPTTTTS PPRPYPELIS 550 560 570 580 590 600 RPSPPTFHRF LPDLPPSRSA VEIAPTQVTE TDECRLNQNI CGHGQCVPGP SDYSCHCNAG 610 620 630 640 650 660 YRSHPQHRYC VDVNECEAEP CGPGKGICMN TGGSYNCHCN RGYRLHVGAG GRSCVDLNEC 670 680 690 700 710 720 AKPHLCGDGG FCINFPGHYK CNCYPGYRLK ASRPPICEDI DECRDPSTCP DGKCENKPGS 730 740 750 760 770 780 FKCIACQPGY RSQGGGACRD VNECSEGTPC SPGWCENLPG SYRCTCAQYE PAQDGLSCID 790 800 810 820 830 840 VDECEAGKVC QDGICTNTPG SFQCQCLSGY HLSRDRSRCE DIDECDFPAA CIGGDCINTN 850 860 870 880 890 900 GSYRCLCPLG HRLVGGRKCK KDIDECSQDP GLCLPHACEN LQGSYVCVCD EGFTLTQDQH 910 920 930 940 950 960 GCEEVEQPHH KKECYLNFDD TVFCDSVLAT NVTQQECCCS LGAGWGDHCE IYPCPVYSSA 970 980 990 1000 1010 1020 EFHSLVPDGK RLHSGQQHCE LCIPAHRDID ECILFGAEIC KEGKCVNTQP GYECYCKQGF 1030 1040 1050 1060 1070 1080 YYDGNLLECV DVDECLDESN CRNGVCENTR GGYRCACTPP AEYSPAQAQC LIPERWSTPQ 1090 1100 1110 1120 1130 1140 RDVKCAGASE ERTACVWGPW AGPALTFDDC CCRQPRLGTQ CRPCPPRGTG SQCPTSQSES 1150 1160 1170 1180 1190 1200 NSFWDTSPLL LGKSPRDEDS SEEDSDECRC VSGRCVPRPG GAVCECPGGF QLDASRARCV 1210 1220 1230 1240 1250 1260 DIDECRELNQ RGLLCKSERC VNTSGSFRCV CKAGFTRSRP HGPACLSAAA DDAAIAHTSV IDHRGYFH
	Isoform 2. Checksum: C5D95F4994A2E776	FASTA	882	93,355
	10 20 30 40 50 60 MPGPRGAAHG LAPAMHQAGA LGLLALLLLA LLGPGGGAEG GPAGERGTGG GGALARERFK 70 80 90 100 110 120 VVFAPVICKR TCLKGQCRDS CQQGSNMTLI GENGHSTDTL TGSAFRVVVC PLPCMNGGQC 130 140 150 160 170 180 SSRNQCLCPP DFTGRFCQVP AAGTGAGTGS SGPGLARTGA MSTGPLPPLA PEGESVASKH 190 200 210 220 230 240 AIYAVQVIAD PPGPGEGPPA QHAAFLVPLG PGQISAEVQA PPPVVNVRVH HPPEASVQVH 250 260 270 280 290 300 RIEGPNAEGP ASSQHLLPHP KPPHPRPPTQ KPLGRCFQDT LPKQPCGSNP LPGLTKQEDC 310 320 330 340 350 360 CGSIGTAWGQ SKCHKCPQLQ YTGVQKPVPV RGEVGADCPQ GYKRLNSTHC QDINECAMPG 370 380 390 400 410 420 NVCHGDCLNN PGSYRCVCPP GHSLGPLAAQ CIADKPEEKS LCFRLVSTEH QCQHPLTTRL 430 440 450 460 470 480 TRQLCCCSVG KAWGARCQRC PADGTAAFKE ICPGKGYHIL TSHQTLTIQG ESDFSLFLHP 490 500 510 520 530 540 DGPPKPQQLP ESPSRAPPLE DTEEERGVTM DPPVSEERSV QQSHPTTTTS PPRPYPELIS 550 560 570 580 590 600 RPSPPTFHRF LPDLPPSRSA VEIAPTQVTE TDECRLNQNI CGHGQCVPGP SDYSCHCNAG 610 620 630 640 650 660 YRSHPQHRYC VDVNECEAEP CGPGKGICMN TGGSYNCHCN RGYRLHVGAG GRSCVDLNEC 670 680 690 700 710 720 AKPHLCGDGG FCINFPGHYK CNCYPGYRLK ASRPPICEDI DECRDPSTCP DGKCENKPGS 730 740 750 760 770 780 FKCIACQPGY RSQGGGACRD VNECSEGTPC SPGWCENLPG SYRCTCAQYE PAQDGLSCID 790 800 810 820 830 840 VDECEAGKVC QDGICTNTPG SFQCQCLSGY HLSRDRSRCE DIDECDFPAA CIGGDCINTN 850 860 870 880 GSYRCLCPLG HRLVGGRKCQ GMRSWPGILK GEAGQCDLFD TL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation of a novel latent transforming growth factor-beta binding protein gene (LTBP-3)." Yin W., Smiley E., Germiller J., Mecham R.P., Florer J.B., Wenstrup R.J., Bonadio J. J. Biol. Chem. 270:10147-10160(1995) [PubMed: 7730318] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: C57BL/6J. Tissue: Adipose tissue.
[3]	"8-cysteine TGF-BP structural motifs are the site of covalent binding between mouse LTBP-3, LTBP-2, and latent TGF-beta 1." Yin W., Fang J., Smiley E., Bonadio J. Biochim. Biophys. Acta 1383:340-350(1998) [PubMed: 9602168] [Abstract] Cited for: INTERACTION WITH TGFB1.
[4]	"Latent TGF-beta binding protein-3 (LTBP-3) requires binding to TGF-beta for secretion." Chen Y., Dabovic B., Annes J.P., Rifkin D.B. FEBS Lett. 517:277-280(2002) [PubMed: 12062452] [Abstract] Cited for: INTERACTION WITH TGFB1.
[5]	"Latent transforming growth factor-beta binding proteins (LTBPs) --structural extracellular matrix proteins for targeting TGF-beta action." Saharinen J., Hyytiainen M., Taipale J., Keski-Oja J. Cytokine Growth Factor Rev. 10:99-117(1999) [PubMed: 10743502] [Abstract] Cited for: REVIEW.
[6]	"The latent transforming growth factor beta binding protein (LTBP) family." Oklu R., Hesketh R. Biochem. J. 352:601-610(2000) [PubMed: 11104663] [Abstract] Cited for: REVIEW.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L40459 mRNA. Translation: AAB53015.1. Sequence problems. AK080869 mRNA. Translation: BAC38053.1.
IPI	IPI00407364. IPI00828508.
PIR	A57293.
RefSeq	NP_032546.2.
UniGene	Mm.182396
3D structure databases
HSSP	HSSP built from PDB template 1BF9 based on UniProtKB P08709.
ModBase	Search...
PTM databases
PhosphoSite	Q61810.
Genome annotation databases
Ensembl	ENSMUSG00000024940. Mus musculus. [Contig view]
GeneID	16998.
KEGG	mmu:16998.
Organism-specific databases
MGI	MGI:1101355. Ltbp3.
Phylogenomic databases
HOGENOM	Q61810.
HOVERGEN	Q61810.
Gene expression databases
ArrayExpress	Q61810.
Bgee	Q61810.
GermOnline	ENSMUSG00000024940. Mus musculus.
Family and domain databases
InterPro	IPR006209. EGF. IPR006210. EGF-like. IPR013032. EGF-like_reg_CS. IPR000152. EGF-type_Asp/Asn_hydroxyl_CS. IPR000742. EGF_3. IPR001881. EGF_Ca_bd. IPR013091. EGF_Ca_bd_2. IPR018097. EGF_Ca_bd_CS. IPR002212. Fibril-assoc. IPR017878. TFG_b-bd. [Graphical view]
Gene3D	G3DSA:3.90.290.10. Fibril-assoc. 2 hits.
Pfam	PF00008. EGF. 1 hit. PF07645. EGF_CA. 11 hits. PF00683. TB. 3 hits. [Graphical view]
SMART	SM00181. EGF. 2 hits. SM00179. EGF_CA. 12 hits. [Graphical view]
PROSITE	PS00010. ASX_HYDROXYL. 10 hits. PS00022. EGF_1. 2 hits. PS01186. EGF_2. 5 hits. PS50026. EGF_3. 12 hits. PS01187. EGF_CA. 11 hits. PS51364. TB. 3 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	291104.
SOURCE	Search...

Entry information

Entry name

LTBP3_MOUSE

Accession

Primary (citable) accession number: Q61810
Secondary accession number(s): Q8BNQ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	January 16, 2004
Last modified:	June 16, 2009
This is version 86 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)