Q61805 (LBP_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lipopolysaccharide-binding protein
Short name=LBP

Gene names

Name:

Lbp

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	481 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria, and acts as an affinity enhancer for CD14, facilitating its association with LPS By similarity.
Subunit structure	When bound to LPS, interacts (via C-terminus) with soluble CD14 By similarity.
Subcellular location	Secreted.
Sequence similarities	Belongs to the BPI/LBP/Plunc superfamily. BPI/LBP family.

Ontologies

Keywords
Biological process	Lipid transport Transport
Cellular component	Secreted
Domain	Signal
Molecular function	Antibiotic Antimicrobial
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	acute-phase response Inferred from mutant phenotype PubMed 12932360. Source: BHF-UCL cellular response to lipoteichoic acid Inferred from electronic annotation. Source: Compara defense response to Gram-negative bacterium Inferred from mutant phenotype PubMed 9338787. Source: BHF-UCL defense response to Gram-positive bacterium Inferred from mutant phenotype PubMed 12932360. Source: BHF-UCL detection of molecule of bacterial origin Inferred from electronic annotation. Source: Compara innate immune response Inferred from mutant phenotype PubMed 12932360. Source: BHF-UCL leukocyte chemotaxis involved in inflammatory response Inferred from mutant phenotype PubMed 12055258. Source: MGI lipopolysaccharide transport Inferred from electronic annotation. Source: Compara lipopolysaccharide-mediated signaling pathway Inferred from electronic annotation. Source: Compara macrophage activation involved in immune response Inferred from direct assay PubMed 12932360. Source: BHF-UCL negative regulation of growth of symbiont in host Inferred from mutant phenotype PubMed 12055258. Source: MGI negative regulation of tumor necrosis factor production Inferred from electronic annotation. Source: Compara opsonization Inferred by curator PubMed 12932360. Source: BHF-UCL positive regulation of chemokine production Inferred from mutant phenotype PubMed 12055258. Source: MGI positive regulation of interleukin-6 production Inferred from electronic annotation. Source: Compara positive regulation of interleukin-8 production Inferred from electronic annotation. Source: Compara positive regulation of macrophage activation Inferred from electronic annotation. Source: Compara positive regulation of neutrophil chemotaxis Inferred from mutant phenotype PubMed 12055258. Source: MGI positive regulation of respiratory burst involved in inflammatory response Inferred from mutant phenotype PubMed 9338787. Source: BHF-UCL positive regulation of toll-like receptor 4 signaling pathway Inferred from electronic annotation. Source: Compara positive regulation of tumor necrosis factor biosynthetic process Inferred from mutant phenotype PubMed 12055258. Source: MGI positive regulation of tumor necrosis factor production Inferred from direct assay PubMed 12932360. Source: BHF-UCL response to lipopolysaccharide Inferred from mutant phenotype PubMed 9338787. Source: BHF-UCL
Cellular_component	extracellular space Inferred from direct assay PubMed 9338787. Source: BHF-UCL
Molecular_function	Gram-negative bacterial cell surface binding Inferred from electronic annotation. Source: Compara Gram-positive bacterial cell surface binding Inferred from direct assay PubMed 12932360. Source: BHF-UCL lipopolysaccharide binding Inferred from direct assay PubMed 11079463. Source: MGI lipoteichoic acid binding Inferred from electronic annotation. Source: Compara receptor binding Inferred from direct assay PubMed 9338787. Source: BHF-UCL
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Chain	25 – 481	457	Lipopolysaccharide-binding protein		PRO_0000017159
Amino acid modifications
	Glycosylation	300	1	N-linked (GlcNAc...) Potential
	Glycosylation	355	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	25	1	G → C in CAA67727. Ref.1
	Sequence conflict	51	1	Q → K in CAA67727. Ref.1
	Sequence conflict	102	1	S → R in CAA67727. Ref.1
	Sequence conflict	280	1	A → S in AAH04795. Ref.3
	Sequence conflict	284	1	Y → H in CAA67727. Ref.1
	Sequence conflict	284	1	Y → H in AAH04795. Ref.3
	Sequence conflict	310	1	H → P in AAH04795. Ref.3
	Sequence conflict	313	1	G → S in AAH04795. Ref.3
	Sequence conflict	341	1	G → R in CAA67727. Ref.1
	Sequence conflict	382	1	G → S in CAA67727. Ref.1
	Sequence conflict	395 – 396	2	NS → TR in CAA67727. Ref.1
	Sequence conflict	418	1	M → I in CAA67727. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q61805 [UniParc]. Last modified July 27, 2011. Version 2. Checksum: AC1D3FC2DA87FE9A	FASTA	481	53,055
	10 20 30 40 50 60 MKAGTGPLLS TLLGLLFLSI QGTGGVNPGV VARITDKGLA YAAKEGLVAL QRELYKITLP 70 80 90 100 110 120 DFSGDFKIKA VGRGQYEFHS LEIQNCELRG SSLKLLPGQG LSLAISDSSI GVRGKWKVRK 130 140 150 160 170 180 SFLKLHGSFD LDVKGVTISV DLLLGMDPSG RPTVSASGCS SRICDLDVHI SGNVGWLLNL 190 200 210 220 230 240 FHNQIESKLQ KVLENKVCEM IQKSVTSDLQ PYLQTLPVTA EIDNVLGIDY SLVAAPQAKA 250 260 270 280 290 300 QVLDVMFKGE IFNRNHRSPV ATPTPTMSLP EDSKQMVYFA ISDYAFNIAS RVYHQAGYLN 310 320 330 340 350 360 FSITDDMLPH DSGIRLNTKA FRPFTPQIYK KYPDMKLELL GTVVSAPILN VSPGNLSLAP 370 380 390 400 410 420 QMEIEGFVIL PTSAREPVFR LGVVTNVFAS LTFNNSKVTG MLHPDKAQVR LIESKVGMFN 430 440 450 460 470 480 VNLFQAFLNY YLLNSLYPDV NAELAQGFPL PLPRHIQLHD LDFQIRKDFL YLGANVQYMR V

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Reactivity of murine and human recombinant LPS-binding protein (LBP) within LPS and Gram-negative bacteria." Lengacher S., Jongeneel C.V., le Roy D., Lee J.D., Kravchenko V., Ulevitch R.J., Glauser M.P., Heumann D. J. Inflamm. 47:165-172(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c.
[2]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[3]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X99347 mRNA. Translation: CAA67727.1. AL663063 Genomic DNA. Translation: CAM23528.1. CH466551 Genomic DNA. Translation: EDL06262.1. BC004795 mRNA. Translation: AAH04795.1.
IPI	IPI00126060.
RefSeq	NP_032515.2. NM_008489.2.
UniGene	Mm.218846.
3D structure databases
ProteinModelPortal	Q61805.
SMR	Q61805. Positions 26-478.
ModBase	Search...
PTM databases
PhosphoSite	Q61805.
Proteomic databases
PaxDb	Q61805.
PRIDE	Q61805.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000016168; ENSMUSP00000016168; ENSMUSG00000016024.
GeneID	16803.
KEGG	mmu:16803.
Organism-specific databases
CTD	3929.
MGI	MGI:1098776. Lbp.
Phylogenomic databases
eggNOG	NOG262970.
GeneTree	ENSGT00620000087589.
HOGENOM	HOG000231250.
HOVERGEN	HBG002797.
InParanoid	A2AC66.
KO	K05399.
OMA	RNHRSPV.
OrthoDB	EOG4JDH6R.
Gene expression databases
ArrayExpress	Q61805.
Bgee	Q61805.
CleanEx	MM_LBP.
Genevestigator	Q61805.
GermOnline	ENSMUSG00000016024. Mus musculus.
Family and domain databases
InterPro	IPR017943. Bactericidal_perm-incr_a/b_dom. IPR001124. Lipid-bd_serum_glycop_C. IPR017954. Lipid-bd_serum_glycop_CS. IPR017942. Lipid-bd_serum_glycop_N. [Graphical view]
Pfam	PF01273. LBP_BPI_CETP. 1 hit. PF02886. LBP_BPI_CETP_C. 1 hit. [Graphical view]
SMART	SM00328. BPI1. 1 hit. SM00329. BPI2. 1 hit. [Graphical view]
SUPFAM	SSF55394. Bactericidal_perm-incr_a/b_dom. 2 hits.
PROSITE	PS00400. LBP_BPI_CETP. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	LBP. mouse.
NextBio	290688.
SOURCE	Search...

Entry information

Entry name

LBP_MOUSE

Accession

Primary (citable) accession number: Q61805
Secondary accession number(s): A2AC66, Q99KA0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	July 27, 2011
Last modified:	May 1, 2013
This is version 100 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program