Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

LIM and SH3 domain protein 1

Gene

Lasp1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Plays an important role in the regulation of dynamic actin-based, cytoskeletal activities. Agonist-dependent changes in LASP1 phosphorylation may also serve to regulate actin-associated ion transport activities, not only in the parietal cell but also in certain other F-actin-rich secretory epithelial cell types (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Ion transport, Transport

Keywords - Ligandi

Actin-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
LIM and SH3 domain protein 1
Short name:
LASP-1
Alternative name(s):
Metastatic lymph node gene 50 protein
Short name:
MLN 50
Gene namesi
Name:Lasp1
Synonyms:Mln50
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:109656. Lasp1.

Subcellular locationi

GO - Cellular componenti

  • cell-cell adherens junction Source: MGI
  • cortical actin cytoskeleton Source: UniProtKB
  • cytoplasm Source: MGI
  • extracellular exosome Source: MGI
  • focal adhesion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000757621 – 263LIM and SH3 domain protein 1Add BLAST263

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei42N6-acetyllysineBy similarity1
Modified residuei68PhosphothreonineBy similarity1
Modified residuei75N6-methyllysineBy similarity1
Modified residuei99PhosphoserineBy similarity1
Modified residuei104PhosphothreonineCombined sources1
Modified residuei112N6-succinyllysineCombined sources1
Modified residuei118PhosphoserineBy similarity1
Modified residuei134PhosphoserineBy similarity1
Modified residuei156Phosphothreonine; by PKA1 Publication1

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

EPDiQ61792.
MaxQBiQ61792.
PaxDbiQ61792.
PRIDEiQ61792.

PTM databases

iPTMnetiQ61792.
PhosphoSitePlusiQ61792.
SwissPalmiQ61792.

Expressioni

Gene expression databases

BgeeiENSMUSG00000038366.
CleanExiMM_LASP1.
ExpressionAtlasiQ61792. baseline and differential.
GenevisibleiQ61792. MM.

Interactioni

Subunit structurei

Interacts with F-actin. Interacts with KBTBD10 (By similarity). Interacts with ANKRD54.By similarity1 Publication

GO - Molecular functioni

  • actin filament binding Source: MGI
  • cadherin binding involved in cell-cell adhesion Source: MGI

Protein-protein interaction databases

BioGridi201111. 7 interactors.
IntActiQ61792. 3 interactors.
MINTiMINT-1862350.
STRINGi10090.ENSMUSP00000042123.

Structurei

3D structure databases

ProteinModelPortaliQ61792.
SMRiQ61792.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 56LIM zinc-bindingPROSITE-ProRule annotationAdd BLAST52
Repeati61 – 95Nebulin 1Add BLAST35
Repeati97 – 131Nebulin 2Add BLAST35
Domaini204 – 263SH3PROSITE-ProRule annotationAdd BLAST60

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi174 – 180Poly-Gln7
Compositional biasi203 – 206Poly-Gly4

Sequence similaritiesi

Contains 1 LIM zinc-binding domain.PROSITE-ProRule annotation
Contains 2 nebulin repeats.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

LIM domain, Repeat, SH3 domain

Phylogenomic databases

eggNOGiKOG1702. Eukaryota.
ENOG4111GQ8. LUCA.
GeneTreeiENSGT00530000062924.
HOGENOMiHOG000006616.
HOVERGENiHBG054636.
InParanoidiQ61792.
OMAiYGYKEPA.
OrthoDBiEOG091G0U2O.
PhylomeDBiQ61792.
TreeFamiTF319104.

Family and domain databases

Gene3Di2.10.110.10. 1 hit.
InterProiIPR000900. Nebulin_repeat.
IPR001452. SH3_domain.
IPR001781. Znf_LIM.
[Graphical view]
PfamiPF00412. LIM. 1 hit.
PF00880. Nebulin. 2 hits.
PF14604. SH3_9. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00132. LIM. 1 hit.
SM00227. NEBU. 2 hits.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS00478. LIM_DOMAIN_1. 1 hit.
PS50023. LIM_DOMAIN_2. 1 hit.
PS51216. NEBULIN. 2 hits.
PS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q61792-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNPNCARCGK IVYPTEKVNC LDKYWHKACF HCETCKMTLN MKNYKGYEKK
60 70 80 90 100
PYCNAHYPKQ SFTMVADTPE NLRLKQQSEL QSQVRYKEEF EKNKGKGFSV
110 120 130 140 150
VADTPELQRI KKTQDQISNI KYHEEFEKSR MGPSGGEGVE PERREAQDSS
160 170 180 190 200
SYRRPTEQQQ PQPHHIPTSA PVYQQPQQQQ MTSSYGGYKE PAAPVSIQRS
210 220 230 240 250
APGGGGKRYR AVYDYSAADE DEVSFQDGDT IVNVQQIDDG WMYGTVERTG
260
DTGMLPANYV EAI
Length:263
Mass (Da):29,994
Last modified:November 1, 1996 - v1
Checksum:iA6CA2FC2E451433E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X96973 mRNA. Translation: CAA65659.1.
AK078445 mRNA. Translation: BAC37278.1.
BC010840 mRNA. Translation: AAH10840.1.
U58882 mRNA. Translation: AAC52639.1.
CCDSiCCDS25332.1.
RefSeqiNP_034818.1. NM_010688.4.
XP_006532404.1. XM_006532341.1.
UniGeneiMm.271967.
Mm.470698.

Genome annotation databases

EnsembliENSMUST00000043843; ENSMUSP00000042123; ENSMUSG00000038366.
GeneIDi16796.
KEGGimmu:16796.
UCSCiuc007lew.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X96973 mRNA. Translation: CAA65659.1.
AK078445 mRNA. Translation: BAC37278.1.
BC010840 mRNA. Translation: AAH10840.1.
U58882 mRNA. Translation: AAC52639.1.
CCDSiCCDS25332.1.
RefSeqiNP_034818.1. NM_010688.4.
XP_006532404.1. XM_006532341.1.
UniGeneiMm.271967.
Mm.470698.

3D structure databases

ProteinModelPortaliQ61792.
SMRiQ61792.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201111. 7 interactors.
IntActiQ61792. 3 interactors.
MINTiMINT-1862350.
STRINGi10090.ENSMUSP00000042123.

PTM databases

iPTMnetiQ61792.
PhosphoSitePlusiQ61792.
SwissPalmiQ61792.

Proteomic databases

EPDiQ61792.
MaxQBiQ61792.
PaxDbiQ61792.
PRIDEiQ61792.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000043843; ENSMUSP00000042123; ENSMUSG00000038366.
GeneIDi16796.
KEGGimmu:16796.
UCSCiuc007lew.1. mouse.

Organism-specific databases

CTDi3927.
MGIiMGI:109656. Lasp1.

Phylogenomic databases

eggNOGiKOG1702. Eukaryota.
ENOG4111GQ8. LUCA.
GeneTreeiENSGT00530000062924.
HOGENOMiHOG000006616.
HOVERGENiHBG054636.
InParanoidiQ61792.
OMAiYGYKEPA.
OrthoDBiEOG091G0U2O.
PhylomeDBiQ61792.
TreeFamiTF319104.

Miscellaneous databases

ChiTaRSiLasp1. mouse.
PROiQ61792.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000038366.
CleanExiMM_LASP1.
ExpressionAtlasiQ61792. baseline and differential.
GenevisibleiQ61792. MM.

Family and domain databases

Gene3Di2.10.110.10. 1 hit.
InterProiIPR000900. Nebulin_repeat.
IPR001452. SH3_domain.
IPR001781. Znf_LIM.
[Graphical view]
PfamiPF00412. LIM. 1 hit.
PF00880. Nebulin. 2 hits.
PF14604. SH3_9. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00132. LIM. 1 hit.
SM00227. NEBU. 2 hits.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS00478. LIM_DOMAIN_1. 1 hit.
PS50023. LIM_DOMAIN_2. 1 hit.
PS51216. NEBULIN. 2 hits.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLASP1_MOUSE
AccessioniPrimary (citable) accession number: Q61792
Secondary accession number(s): Q62416
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 147 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.