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Protein

Lymphocyte activation gene 3 protein

Gene

Lag3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in lymphocyte activation. Binds to HLA class-II antigens (By similarity).By similarity

GO - Molecular functioni

  • MHC class II protein binding Source: MGI
  • transmembrane signaling receptor activity Source: MGI

GO - Biological processi

  • cell surface receptor signaling pathway Source: MGI
  • negative regulation of interleukin-2 biosynthetic process Source: MGI
  • negative regulation of T cell activation Source: MGI
  • positive regulation of natural killer cell mediated cytotoxicity Source: MGI
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Lymphocyte activation gene 3 protein
Short name:
LAG-3
Alternative name(s):
CD_antigen: CD223
Gene namesi
Name:Lag3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:106588. Lag3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini23 – 442420ExtracellularSequence analysisAdd
BLAST
Transmembranei443 – 46321HelicalSequence analysisAdd
BLAST
Topological domaini464 – 52158CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • external side of plasma membrane Source: MGI
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence analysisAdd
BLAST
Chaini23 – 521499Lymphocyte activation gene 3 proteinPRO_0000014632Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi44 ↔ 156PROSITE-ProRule annotation
Glycosylationi184 – 1841N-linked (GlcNAc...)Sequence analysis
Disulfide bondi185 ↔ 235PROSITE-ProRule annotation
Glycosylationi244 – 2441N-linked (GlcNAc...)Sequence analysis
Disulfide bondi276 ↔ 327PROSITE-ProRule annotation
Glycosylationi309 – 3091N-linked (GlcNAc...)Sequence analysis
Glycosylationi337 – 3371N-linked (GlcNAc...)Sequence analysis
Disulfide bondi363 ↔ 405PROSITE-ProRule annotation
Glycosylationi381 – 3811N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiQ61790.
MaxQBiQ61790.
PaxDbiQ61790.
PRIDEiQ61790.

PTM databases

iPTMnetiQ61790.
PhosphoSiteiQ61790.

Miscellaneous databases

PMAP-CutDBQ61790.

Expressioni

Gene expression databases

BgeeiQ61790.
CleanExiMM_LAG3.
GenevisibleiQ61790. MM.

Interactioni

GO - Molecular functioni

  • MHC class II protein binding Source: MGI

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000032217.

Structurei

3D structure databases

ProteinModelPortaliQ61790.
SMRiQ61790. Positions 119-293, 309-344.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini37 – 163127Ig-like V-typeAdd
BLAST
Domaini165 – 24682Ig-like C2-type 1Add
BLAST
Domaini258 – 34184Ig-like C2-type 2Add
BLAST
Domaini345 – 41268Ig-like C2-type 3Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni493 – 5182613 X 2 AA tandem repeats of E-XAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IFPP. Eukaryota.
ENOG410Z3KP. LUCA.
GeneTreeiENSGT00390000017331.
HOGENOMiHOG000113275.
HOVERGENiHBG004952.
InParanoidiQ61790.
KOiK06565.
OMAiERFVWSP.
OrthoDBiEOG718KC5.
PhylomeDBiQ61790.
TreeFamiTF335942.

Family and domain databases

Gene3Di2.60.40.10. 4 hits.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR015621. IL-1_rcpt_fam.
IPR013151. Immunoglobulin.
[Graphical view]
PANTHERiPTHR11890. PTHR11890. 2 hits.
PfamiPF00047. ig. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 3 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 4 hits.
PROSITEiPS50835. IG_LIKE. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q61790-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MREDLLLGFL LLGLLWEAPV VSSGPGKELP VVWAQEGAPV HLPCSLKSPN
60 70 80 90 100
LDPNFLRRGG VIWQHQPDSG QPTPIPALDL HQGMPSPRQP APGRYTVLSV
110 120 130 140 150
APGGLRSGRQ PLHPHVQLEE RGLQRGDFSL WLRPALRTDA GEYHATVRLP
160 170 180 190 200
NRALSCSLRL RVGQASMIAS PSGVLKLSDW VLLNCSFSRP DRPVSVHWFQ
210 220 230 240 250
GQNRVPVYNS PRHFLAETFL LLPQVSPLDS GTWGCVLTYR DGFNVSITYN
260 270 280 290 300
LKVLGLEPVA PLTVYAAEGS RVELPCHLPP GVGTPSLLIA KWTPPGGGPE
310 320 330 340 350
LPVAGKSGNF TLHLEAVGLA QAGTYTCSIH LQGQQLNATV TLAVITVTPK
360 370 380 390 400
SFGLPGSRGK LLCEVTPASG KERFVWRPLN NLSRSCPGPV LEIQEARLLA
410 420 430 440 450
ERWQCQLYEG QRLLGATVYA AESSSGAHSA RRISGDLKGG HLVLVLILGA
460 470 480 490 500
LSLFLLVAGA FGFHWWRKQL LLRRFSALEH GIQPFPAQRK IEELERELET
510 520
EMGQEPEPEP EPQLEPEPRQ L
Length:521
Mass (Da):56,978
Last modified:November 1, 1996 - v1
Checksum:i64C29C40EB780E48
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X98113 mRNA. Translation: CAA66794.1.
BC120591 mRNA. Translation: AAI20592.1.
CCDSiCCDS20536.1.
RefSeqiNP_032505.1. NM_008479.2.
UniGeneiMm.4528.

Genome annotation databases

EnsembliENSMUST00000032217; ENSMUSP00000032217; ENSMUSG00000030124.
GeneIDi16768.
KEGGimmu:16768.
UCSCiuc009dsl.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X98113 mRNA. Translation: CAA66794.1.
BC120591 mRNA. Translation: AAI20592.1.
CCDSiCCDS20536.1.
RefSeqiNP_032505.1. NM_008479.2.
UniGeneiMm.4528.

3D structure databases

ProteinModelPortaliQ61790.
SMRiQ61790. Positions 119-293, 309-344.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000032217.

PTM databases

iPTMnetiQ61790.
PhosphoSiteiQ61790.

Proteomic databases

EPDiQ61790.
MaxQBiQ61790.
PaxDbiQ61790.
PRIDEiQ61790.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000032217; ENSMUSP00000032217; ENSMUSG00000030124.
GeneIDi16768.
KEGGimmu:16768.
UCSCiuc009dsl.2. mouse.

Organism-specific databases

CTDi3902.
MGIiMGI:106588. Lag3.

Phylogenomic databases

eggNOGiENOG410IFPP. Eukaryota.
ENOG410Z3KP. LUCA.
GeneTreeiENSGT00390000017331.
HOGENOMiHOG000113275.
HOVERGENiHBG004952.
InParanoidiQ61790.
KOiK06565.
OMAiERFVWSP.
OrthoDBiEOG718KC5.
PhylomeDBiQ61790.
TreeFamiTF335942.

Miscellaneous databases

PMAP-CutDBQ61790.
PROiQ61790.
SOURCEiSearch...

Gene expression databases

BgeeiQ61790.
CleanExiMM_LAG3.
GenevisibleiQ61790. MM.

Family and domain databases

Gene3Di2.60.40.10. 4 hits.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR015621. IL-1_rcpt_fam.
IPR013151. Immunoglobulin.
[Graphical view]
PANTHERiPTHR11890. PTHR11890. 2 hits.
PfamiPF00047. ig. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 3 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 4 hits.
PROSITEiPS50835. IG_LIKE. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of murine LAG-3 by magnetic bead bound homologous probes and PCR (gene-capture PCR)."
    Mastrangeli R., Micangeli E., Donini S.
    Anal. Biochem. 241:93-102(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/cJ.
    Tissue: Thymus.
  2. "Independent modes of natural killing distinguished in mice lacking Lag3."
    Miyazaki T., Dierich A., Benoist C., Mathis D.
    Science 272:405-408(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/cJ.
    Tissue: Thymus.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.

Entry informationi

Entry nameiLAG3_MOUSE
AccessioniPrimary (citable) accession number: Q61790
Secondary accession number(s): Q0VBL2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: June 8, 2016
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.