Reviewed, UniProtKB/Swiss-Prot Q61790 (LAG3_MOUSE)

Names and origin

Protein names

Recommended name:
    Lymphocyte activation gene 3 protein
      Short name=LAG-3
Alternative name(s):
    CD_antigen=CD223

Gene names

Name:

Lag3

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	521 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Involved in lymphocyte activation. Binds to HLA class-II antigens By similarity.
Subcellular location	Membrane; Single-pass type I membrane protein.
Sequence similarities	Contains 3 Ig-like C2-type (immunoglobulin-like) domains. Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Ontologies

Keywords
Cellular component	Membrane
Domain	Immunoglobulin domain Repeat Signal Transmembrane
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological process	cell surface receptor linked signal transduction Inferred from direct assay. Source: MGI negative regulation of T cell activation Inferred from direct assay. Source: MGI negative regulation of interleukin-2 biosynthetic process Inferred from direct assay. Source: MGI positive regulation of natural killer cell mediated cytotoxicity Ref.2 Inferred from direct assay. Source: MGI
Cellular component	external side of plasma membrane Ref.2 Inferred from direct assay. Source: MGI integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	MHC class II protein binding Inferred from direct assay. Source: MGI transmembrane receptor activity Inferred from direct assay. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 521	499	Lymphocyte activation gene 3 protein		PRO_0000014632
Regions
	Topological domain	23 – 442	420	Extracellular Potential
	Transmembrane	443 – 463	21	Potential
	Topological domain	464 – 521	58	Cytoplasmic Potential
	Domain	37 – 163	127	Ig-like V-type
	Domain	165 – 246	82	Ig-like C2-type 1
	Domain	258 – 341	84	Ig-like C2-type 2
	Domain	345 – 412	68	Ig-like C2-type 3
	Region	493 – 518	26	13 X 2 AA tandem repeats of E-X
Amino acid modifications
	Glycosylation	184	1	N-linked (GlcNAc...) Potential
	Glycosylation	244	1	N-linked (GlcNAc...) Potential
	Glycosylation	309	1	N-linked (GlcNAc...) Potential
	Glycosylation	337	1	N-linked (GlcNAc...) Potential
	Glycosylation	381	1	N-linked (GlcNAc...) Potential
	Disulfide bond	44 ↔ 156		Potential
	Disulfide bond	185 ↔ 235		Potential
	Disulfide bond	276 ↔ 327		Potential
	Disulfide bond	363 ↔ 405		Potential

Sequences

	Sequence		Length	Mass (Da)
	Q61790-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 64C29C40EB780E48	FASTA	521	56,978
	10 20 30 40 50 60 MREDLLLGFL LLGLLWEAPV VSSGPGKELP VVWAQEGAPV HLPCSLKSPN LDPNFLRRGG 70 80 90 100 110 120 VIWQHQPDSG QPTPIPALDL HQGMPSPRQP APGRYTVLSV APGGLRSGRQ PLHPHVQLEE 130 140 150 160 170 180 RGLQRGDFSL WLRPALRTDA GEYHATVRLP NRALSCSLRL RVGQASMIAS PSGVLKLSDW 190 200 210 220 230 240 VLLNCSFSRP DRPVSVHWFQ GQNRVPVYNS PRHFLAETFL LLPQVSPLDS GTWGCVLTYR 250 260 270 280 290 300 DGFNVSITYN LKVLGLEPVA PLTVYAAEGS RVELPCHLPP GVGTPSLLIA KWTPPGGGPE 310 320 330 340 350 360 LPVAGKSGNF TLHLEAVGLA QAGTYTCSIH LQGQQLNATV TLAVITVTPK SFGLPGSRGK 370 380 390 400 410 420 LLCEVTPASG KERFVWRPLN NLSRSCPGPV LEIQEARLLA ERWQCQLYEG QRLLGATVYA 430 440 450 460 470 480 AESSSGAHSA RRISGDLKGG HLVLVLILGA LSLFLLVAGA FGFHWWRKQL LLRRFSALEH 490 500 510 520 GIQPFPAQRK IEELERELET EMGQEPEPEP EPQLEPEPRQ L

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of murine LAG-3 by magnetic bead bound homologous probes and PCR (gene-capture PCR)." Mastrangeli R., Micangeli E., Donini S. Anal. Biochem. 241:93-102(1996) [PubMed: 8921170] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c. Tissue: Thymus.
[2]	"Independent modes of natural killing distinguished in mice lacking Lag3." Miyazaki T., Dierich A., Benoist C., Mathis D. Science 272:405-408(1996) [PubMed: 8602528] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c. Tissue: Thymus.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X98113 mRNA. Translation: CAA66794.1. BC120591 mRNA. Translation: AAI20592.1.
IPI	IPI00125089.
RefSeq	NP_032505.1.
UniGene	Mm.4528
3D structure databases
SMR	Q61790. Positions 31-254, 136-377, 261-412.
ModBase	Search...
Protein-protein interaction databases
STRING	Q61790.
Proteomic databases
PRIDE	Q61790.
Genome annotation databases
Ensembl	ENSMUST00000032217; ENSMUSP00000032217; ENSMUSG00000030124; Mus musculus. [Genome view]
GeneID	16768.
KEGG	mmu:16768.
UCSC	uc009dsl.1. mouse.
Organism-specific databases
CTD	16768.
MGI	MGI:106588. Lag3.
Phylogenomic databases
eggNOG	roNOG09671.
HOGENOM	HBG126621.
HOVERGEN	Q61790.
InParanoid	Q61790.
OMA	AGDNGNF.
OrthoDB	EOG9BP3XP.
PhylomeDB	Q61790.
Gene expression databases
ArrayExpress	Q61790.
Bgee	Q61790.
CleanEx	MM_LAG3.
Genevestigator	Q61790.
GermOnline	ENSMUSG00000030124. Mus musculus.
Family and domain databases
InterPro	IPR007110. Ig-like. IPR013783. Ig-like_fold. IPR003599. Ig_sub. IPR015621. IL1-receptor. [Graphical view]
Gene3D	G3DSA:2.60.40.10. Ig-like_fold. 2 hits.
PANTHER	PTHR11890. IL1-receptor. 1 hit.
SMART	SM00409. IG. 3 hits. [Graphical view]
PROSITE	PS50835. IG_LIKE. 2 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	290590.
PMAP-CutDB	Q61790.
SOURCE	Search...

Entry information

Entry name

LAG3_MOUSE

Accession

Primary (citable) accession number: Q61790
Secondary accession number(s): Q0VBL2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	January 19, 2010
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)