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Reviewed, UniProtKB/Swiss-Prot Q61772 (EPHA7_MOUSE)

Last modified February 9, 2010. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Ephrin type-A receptor 7
    EC=2.7.10.1
Alternative name(s):
    EPH homology kinase 3
      Short name=EHK-3
    Embryonic brain kinase
      Short name=EBK
    Developmental kinase 1
      Short name=mDK-1
Gene names
Name: Epha7
Synonyms: Ebk, Ehk3, Mdk1
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

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Protein attributes

Sequence length998 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Receptor for members of the ephrin-A family. Binds to ephrin-A1, -A2, -A3, -A4 and -A5.

Catalytic activity

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Subunit structure

Interacts with PRKCABP and GRIP1. Ref.4

Subcellular location

Membrane; Single-pass type I membrane protein.

Tissue specificity

Widely expressed in embryo. In adult, expression restricted to hippocampus, testis and spleen.

Sequence similarities

Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.

Contains 2 fibronectin type-III domains.

Contains 1 protein kinase domain.

Contains 1 SAM (sterile alpha motif) domain.

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Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q61772-1)

Also known as: MDK1;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q61772-2)

Also known as: MDK1-1; Ebk-td1;

The sequence of this isoform differs from the canonical sequence as follows:
     540-544: Missing.
Isoform 3 (identifier: Q61772-3)

Also known as: MDK1-2;

The sequence of this isoform differs from the canonical sequence as follows:
     601-604: Missing.
Isoform 4 (identifier: Q61772-4)

Also known as: MDK1-T1;

The sequence of this isoform differs from the canonical sequence as follows:
     600-610: FKFPGTKTYID → SLVTNEHLSVL
     611-998: Missing.
Isoform 5 (identifier: Q61772-5)

Also known as: MDK1-T2;

The sequence of this isoform differs from the canonical sequence as follows:
     600-626: FKFPGTKTYIDPETYEDPNRAVHQFAK → SLYRERGDGMEKTQHNKKWMIASCSRL
     627-998: Missing.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2929 Potential
Chain30 – 998969Ephrin type-A receptor 7
PRO_0000016819

Regions

Topological domain30 – 556527Extracellular Potential
Transmembrane557 – 57721 Potential
Topological domain578 – 998421Cytoplasmic Potential
Domain331 – 433103Fibronectin type-III 1
Domain443 – 53593Fibronectin type-III 2
Domain633 – 894262Protein kinase
Domain923 – 98765SAM
Nucleotide binding639 – 6479ATP By similarity
Motif996 – 9983PDZ-binding Potential
Compositional bias192 – 328137Cys-rich

Sites

Active site7581Proton acceptor By similarity
Binding site6651ATP By similarity

Amino acid modifications

Modified residue6081Phosphotyrosine; by autocatalysis Potential
Modified residue6141Phosphotyrosine; by autocatalysis Potential
Modified residue7911Phosphotyrosine; by autocatalysis Potential
Modified residue9401Phosphotyrosine; by autocatalysis Potential
Glycosylation641N-linked (GlcNAc...) Potential
Glycosylation3431N-linked (GlcNAc...) Potential
Glycosylation4101N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence540 – 5445Missing in isoform 2.
VSP_003006
Alternative sequence600 – 62627FKFPG…HQFAK → SLYRERGDGMEKTQHNKKWM IASCSRL in isoform 5.
VSP_003010
Alternative sequence600 – 61011FKFPGTKTYID → SLVTNEHLSVL in isoform 4.
VSP_003008
Alternative sequence601 – 6044Missing in isoform 3.
VSP_003007
Alternative sequence611 – 998388Missing in isoform 4.
VSP_003009
Alternative sequence627 – 998372Missing in isoform 5.
VSP_003011

Experimental info

Sequence conflict4801Y → H in CAA57224. Ref.3

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Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (MDK1) [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 81C5538E15AEA2FA

FASTA998111,874
        10         20         30         40         50         60 
MVVQTRFPSW IILCYIWLLG FAHTGEAQAA KEVLLLDSKA QQTELEWISS PPSGWEEISG 

        70         80         90        100        110        120 
LDENYTPIRT YQVCQVMEPN QNNWLRTNWI SKGNAQRIFV ELKFTLRDCN SLPGVLGTCK 

       130        140        150        160        170        180 
ETFNLYYYET DYDTGRNIRE NLYVKIDTIA ADESFTQGDL GERKMKLNTE VREIGPLSKK 

       190        200        210        220        230        240 
GFYLAFQDVG ACIALVSVKV YYKKCWTIVE NLAVFPDTVT GSEFSSLVEV RGTCVSSAEE 

       250        260        270        280        290        300 
EAENSPRMHC SAEGEWLVPI GKCICKAGYQ QKGDTCEPCG RRFYKSSSQD LQCSRCPTHS 

       310        320        330        340        350        360 
FSDREGSSRC ECEDGYYRAP SDPPYVACTR PPSAPQNLIF NINQTTVSLE WSPPADNGGR 

       370        380        390        400        410        420 
NDVTYRILCK RCSWEQGECV PCGSNIGYMP QQTGLEDNYV TVMDLLAHAN YTFEVEAVNG 

       430        440        450        460        470        480 
VSDLSRSQRL FAAVSITTGQ AAPSQVSGVM KERVLQRSVQ LSWQEPEHPN GVITEYEIKY 

       490        500        510        520        530        540 
YEKDQRERTY STLKTKSTSA SINNLKPGTV YVFQIRAVTA AGYGNYSPRL DVATLEEASG 

       550        560        570        580        590        600 
KMFEATAVSS EQNPVIIIAV VAVAGTIILV FMVFGFIIGR RHCGYSKADQ EGDEELYFHF 

       610        620        630        640        650        660 
KFPGTKTYID PETYEDPNRA VHQFAKELDA SCIKIERVIG AGEFGEVCSG RLKLPGQRDV 

       670        680        690        700        710        720 
AVAIKTLKVG YTEKQRRDFL CEASIMGQFD HPNVVHLEGV VTRGKPVMIV IEFMENGALD 

       730        740        750        760        770        780 
AFLRKHDGQF TVIQLVGMLR GIAAGMRYLA DMGYVHRDLA ARNILVNSNL VCKVSDFGLS 

       790        800        810        820        830        840 
RVIEDDPEAV YTTTGGKIPV RWTAPEAIQY RKFTSASDVW SYGIVMWEVM SYGERPYWDM 

       850        860        870        880        890        900 
SNQDVIKAIE EGYRLPAPMD CPAGLHQLML DCWQKDRAER PKFEQIVGIL DKMIRNPSSL 

       910        920        930        940        950        960 
KTPLGTCSRP LSPLLDQSTP DFTAFCSVGE WLQAIKMERY KDNFTAAGYN SLESVARMTI 

       970        980        990 
DDVMSLGITL VGHQKKIMSS IQTMRAQMLH LHGTGIQV

« Hide

Isoform 2 (MDK1-1) (Ebk-td1).

Checksum: AE30001D73FC6498
Show »

FASTA993111,282
Isoform 3 (MDK1-2).

Checksum: 83574218B40A07A3
Show »

FASTA994111,445
Isoform 4 (MDK1-T1).

Checksum: 90BD9C39C35AEC1F
Show »

FASTA61068,300
Isoform 5 (MDK1-T2).

Checksum: B6A04E5A7590C8AC
Show »

FASTA62670,314

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References

« Hide 'large scale' references
[1]"Identification of alternatively spliced mRNAs encoding variants of MDK1, a novel receptor tyrosine kinase expressed in the murine nervous system."
Ciossek T., Millauer B., Ullrich A.
Oncogene 10:97-108(1995) [PubMed: 7824284] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING.
Strain: BALB/c.
Tissue: Brain.
[2]"A novel truncated variant form of Ebk/MDK1 receptor tyrosine kinase is expressed in embryonic mouse brain."
Talukder A.H., Muramatsu T., Kaneda N.
Cell Struct. Funct. 22:477-485(1997) [PubMed: 9368721] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[3]"Embryo brain kinase: a novel gene of the eph/elk receptor tyrosine kinase family."
Ellis J., Liu Q., Breitman M., Jenkins N.A., Gilbert D.J., Copeland N.G., Tempest H.V., Warren S., Muir E., Schilling H., Fletcher F.A., Ziegler S.F., Rogers J.H.
Mech. Dev. 52:319-341(1995) [PubMed: 8541219] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 431-998 (ISOFORM 1).
Tissue: Brain.
[4]"PDZ proteins bind, cluster, and synaptically colocalize with Eph receptors and their ephrin ligands."
Torres R., Firestein B.L., Dong H., Staudinger J., Olson E.N., Huganir R.L., Bredt D.S., Gale N.W., Yancopoulos G.D.
Neuron 21:1453-1463(1998) [PubMed: 9883737] [Abstract]
Cited for: INTERACTION WITH PRKCABP AND GRIP1.
[5]"Multiple reaction monitoring for robust quantitative proteomic analysis of cellular signaling networks."
Wolf-Yadlin A., Hautaniemi S., Lauffenburger D.A., White F.M.
Proc. Natl. Acad. Sci. U.S.A. 104:5860-5865(2007) [PubMed: 17389395] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-791, MASS SPECTROMETRY.
+Additional computationally mapped references.

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X79082 mRNA. Translation: CAA55687.1.
X79083 mRNA. Translation: CAA55688.1.
X79084 mRNA. Translation: CAA55689.1.
X81466 mRNA. Translation: CAA57224.1.
IPIIPI00124993.
IPI00343893.
IPI00380199.
IPI00380200.
IPI00648754.
PIRI48612.
I48614.
JC5672.
UniGeneMm.257266

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRINGQ61772.

PTM databases

PhosphoSiteQ61772.

Proteomic databases

PRIDEQ61772.

Genome annotation databases

EnsemblENSMUST00000029964; ENSMUSP00000029964; ENSMUSG00000028289; Mus musculus. [Genome view]
ENSMUST00000080934; ENSMUSP00000079735; ENSMUSG00000028289; Mus musculus. [Genome view]
ENSMUST00000108194; ENSMUSP00000103829; ENSMUSG00000028289; Mus musculus. [Genome view]
UCSCuc008sen.1. mouse.

Organism-specific databases

MGIMGI:95276. Epha7.

Phylogenomic databases

HOGENOMHBG755340.
HOVERGENQ61772.
InParanoidQ61772.

Enzyme and pathway databases

BRENDA2.7.10.1. 244.

Gene expression databases

ArrayExpressQ61772.
BgeeQ61772.
CleanExMM_EPHA7.
GenevestigatorQ61772.
GermOnlineENSMUSG00000028289. Mus musculus.

Family and domain databases

InterProIPR001090. Ephrin_rcpt_lig-bd.
IPR008957. Fibronectin_typ-III-like_fold.
IPR003961. FN_III.
IPR003962. FnIII_subd.
IPR008979. Galactose-bd-like.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_cat_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001660. SAM.
IPR010993. SAM_homology.
IPR013761. SAM_type.
IPR021129. SAM_type1.
IPR020635. Tyr_Pkinase_cat_dom.
IPR020685. Tyr_prot_kinase.
IPR008266. Tyr_prot_kinase_AS.
IPR016257. Tyr_prot_kinase_ephrin_rcpt.
IPR001426. Tyr_prot_kinase_rcpt_V_CS.
[Graphical view]
Gene3DG3DSA:2.60.40.30. FN_III-like. 2 hits.
G3DSA:1.10.150.50. SAM_type. 1 hit.
PANTHERPTHR23256. Tyr_prot_kinase. 1 hit.
PfamPF01404. Ephrin_lbd. 1 hit.
PF00041. fn3. 2 hits.
PF00536. SAM_1. 1 hit.
[Graphical view]
PIRSFPIRSF000666. TyrPK_ephrin_receptor. 1 hit.
PRINTSPR00014. FNTYPEIII.
SMARTSM00615. EPH_lbd. 1 hit.
SM00060. FN3. 2 hits.
SM00454. SAM. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]
PROSITEPS01186. EGF_2. 1 hit. Uncertain.
PS50853. FN3. 2 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS00790. RECEPTOR_TYR_KIN_V_1. 1 hit.
PS00791. RECEPTOR_TYR_KIN_V_2. 1 hit.
PS50105. SAM_DOMAIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

SOURCESearch...

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Entry information

Entry nameEPHA7_MOUSE
AccessionPrimary (citable) accession number: Q61772
Secondary accession number(s): Q61505, Q61773, Q61774
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: February 9, 2010
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents