Q61768 (KINH_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Kinesin-1 heavy chain

Alternative name(s):
Conventional kinesin heavy chain
Ubiquitous kinesin heavy chain
Short name=UKHC

Gene names

Name:	Kif5b
Synonyms:	Khcs, Kns1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	963 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes. May be involved in the mechanisms of growth arrest induced by exposure to DNA-damaging drugs or by cellular senescence. Ref.5
Subunit structure	Interacts with SYBU By similarity. Interacts with PLEKHM2 By similarity. Interacts with ECM29 By similarity. Oligomer composed of two heavy chains and two light chains. Interacts with GRIP1. Interacts with JAKMIP1 and PPP1R42. Ref.6 Ref.7 Ref.8
Subcellular location	Cytoplasm › cytoskeleton By similarity. Note: Uniformly distributed between soma and neurites in hippocampal neurons By similarity.
Domain	Composed of three structural domains: a large globular N-terminal domain which is responsible for the motor activity of kinesin (it hydrolyzes ATP and binds microtubule), a central alpha-helical coiled coil domain that mediates the heavy chain dimerization; and a small globular C-terminal domain which interacts with other proteins (such as the kinesin light chains), vesicles and membranous organelles.
Disruption phenotype	Mice are embryonic lethal. They show perinuclear clustering of mitochondria and impaired lysosomal dispersion. Ref.5
Sequence similarities	Belongs to the kinesin-like protein family. Kinesin subfamily. Contains 1 kinesin-motor domain.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton Microtubule
Domain	Coiled coil
Ligand	ATP-binding Nucleotide-binding
Molecular function	Motor protein
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cytoplasm organization Inferred from mutant phenotype Ref.5. Source: MGI mitochondrial transport Traceable author statement PubMed 11486041. Source: MGI positive regulation of establishment of protein localization to plasma membrane Inferred from electronic annotation. Source: Compara positive regulation of potassium ion transport Inferred from electronic annotation. Source: Compara regulation of membrane potential Inferred from electronic annotation. Source: Compara stress granule disassembly Inferred from mutant phenotype PubMed 19825938. Source: BHF-UCL vesicle transport along microtubule Inferred from sequence or structural similarity. Source: HGNC
Cellular_component	ciliary rootlet Inferred from direct assay PubMed 16018997. Source: MGI cytosol Traceable author statement. Source: Reactome kinesin complex Traceable author statement PubMed 10964943. Source: MGI microtubule Inferred from electronic annotation. Source: UniProtKB-KW neuron projection Inferred from direct assay PubMed 16301330. Source: MGI perinuclear region of cytoplasm Inferred from sequence or structural similarity. Source: HGNC vesicle Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW microtubule binding Inferred from sequence or structural similarity. Source: HGNC microtubule motor activity Inferred from sequence or structural similarity. Source: HGNC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 963	963	Kinesin-1 heavy chain		PRO_0000125352
Regions
	Domain	2 – 328	327	Kinesin-motor
	Nucleotide binding	85 – 92	8	ATP By similarity
	Region	915 – 963	49	Globular
	Coiled coil	329 – 914	586
Amino acid modifications
	Modified residue	933	1	Phosphoserine By similarity
Experimental info
	Sequence conflict	20 – 21	2	ES → AT in AAA20133. Ref.3
	Sequence conflict	40	1	M → V in AAB53940. Ref.1
	Sequence conflict	75	1	E → G in AAA20133. Ref.3
	Sequence conflict	191	1	H → D in AAA20133. Ref.3
	Sequence conflict	255	1	N → K in AAA20133. Ref.3
	Sequence conflict	396	1	I → T in AAA20133. Ref.3
	Sequence conflict	403	1	P → G in AAB53940. Ref.1
	Sequence conflict	451	1	T → I in AAA20132. Ref.3
	Sequence conflict	520	1	S → T in AAB53940. Ref.1
	Sequence conflict	523	1	L → F in AAB53940. Ref.1
	Sequence conflict	653	1	V → D in AAB53940. Ref.1
	Sequence conflict	664	1	V → L in AAB53940. Ref.1
	Sequence conflict	744	1	Q → T in AAB53940. Ref.1
	Sequence conflict	814	1	S → R in AAA20133. Ref.3
	Sequence conflict	865	1	K → F in AAB53940. Ref.1
	Sequence conflict	881	1	L → R in AAA20133. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q61768 [UniParc]. Last modified July 27, 2011. Version 3. Checksum: C3D3461A0C314901	FASTA	963	109,551
	10 20 30 40 50 60 MADPAECNIK VMCRFRPLNE SEVNRGDKYV AKFQGEDTVM IASKPYAFDR VFQSSTSQEQ 70 80 90 100 110 120 VYNDCAKKIV KDVLEGYNGT IFAYGQTSSG KTHTMEGKLH DPEGMGIIPR IVQDIFNYIY 130 140 150 160 170 180 SMDENLEFHI KVSYFEIYLD KIRDLLDVSK TNLSVHEDKN RVPYVKGCTE RFVCSPDEVM 190 200 210 220 230 240 DTIDEGKSNR HVAVTNMNEH SSRSHSIFLI NVKQENTQTE QKLSGKLYLV DLAGSEKVSK 250 260 270 280 290 300 TGAEGAVLDE AKNINKSLSA LGNVISALAE GSTYVPYRDS KMTRILQDSL GGNCRTTIVI 310 320 330 340 350 360 CCSPSSYNES ETKSTLLFGQ RAKTIKNTVC VNVELTAEQW KKKYEKEKEK NKTLRNTIQW 370 380 390 400 410 420 LENELNRWRN GETVPIDEQF DKEKANLEAF TADKDIAITS DKPAAAVGMA GSFTDAERRK 430 440 450 460 470 480 CEEELAKLYK QLDDKDEEIN QQSQLVEKLK TQMLDQEELL ASTRRDQDNM QAELNRLQAE 490 500 510 520 530 540 NDASKEEVKE VLQALEELAV NYDQKSQEVE DKTKEYELLS DELNQKSATL ASIDAELQKL 550 560 570 580 590 600 KEMTNHQKKR AAEMMASLLK DLAEIGIAVG NNDVKQPEGT GMIDEEFTVA RLYISKMKSE 610 620 630 640 650 660 VKTMVKRCKQ LESTQTESNK KMEENEKELA ACQLRISQHE AKIKSLTEYL QNVEQKKRQL 670 680 690 700 710 720 EESVDSLGEE LVQLRAQEKV HEMEKEHLNK VQTANEVKQA VEQQIQSHRE THQKQISSLR 730 740 750 760 770 780 DEVEAKEKLI TDLQDQNQKM VLEQERLRVE HERLKATDQE KSRKLHELTV MQDRREQARQ 790 800 810 820 830 840 DLKGLEETVA KELQTLHNLR KLFVQDLATR VKKSAEVDSD DTGGSAAQKQ KISFLENNLE 850 860 870 880 890 900 QLTKVHKQLV RDNADLRCEL PKLEKRLRAT AERVKALESA LKEAKENASR DRKRYQQEVD 910 920 930 940 950 960 RIKEAVRSKN MARRGHSAQI AKPIRPGQHP AASPTHPGTV RGGGSFVQNN QPVGLRGGGG KQS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Suppression of the expression of a pancreatic beta-cell form of the kinesin heavy chain by antisense oligonucleotides inhibits insulin secretion from primary cultures of mouse beta-cells." Meng Y.X., Wilson G.W., Avery M.C., Varden C.H., Balczon R. Endocrinology 138:1979-1987(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Pancreas.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Brain.
[3]	"Cloning mammalian genes by expression selection of genetic suppressor elements: association of kinesin with drug resistance and cell immortalization." Gudkov A.V., Kazarov A.R., Thimmapaya R., Axenovich S.A., Mazo I.A., Roninson I.B. Proc. Natl. Acad. Sci. U.S.A. 91:3744-3748(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-881. Strain: BALB/c.
[4]	Lubec G., Sunyer B., Chen W.-Q. Submitted (JAN-2009) to UniProtKB Cited for: PROTEIN SEQUENCE OF 132-141 AND 490-505, MASS SPECTROMETRY. Strain: OF1. Tissue: Hippocampus.
[5]	"Targeted disruption of mouse conventional kinesin heavy chain, kif5B, results in abnormal perinuclear clustering of mitochondria." Tanaka Y., Kanai Y., Okada Y., Nonaka S., Takeda S., Harada A., Hirokawa N. Cell 93:1147-1158(1998) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[6]	"Glutamate-receptor-interacting protein GRIP1 directly steers kinesin to dendrites." Setou M., Seog D.-H., Tanaka Y., Kanai Y., Takei Y., Kawagishi M., Hirokawa N. Nature 417:83-87(2002) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH GRIP1.
[7]	"Marlin-1 and conventional kinesin link GABAB receptors to the cytoskeleton and regulate receptor transport." Vidal R.L., Ramirez O.A., Sandoval L., Koenig-Robert R., Haertel S., Couve A. Mol. Cell. Neurosci. 35:501-512(2007) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH JAKMIP1.
[8]	"TLRR (lrrc67) interacts with PP1 and is associated with a cytoskeletal complex in the testis." Wang R., Kaul A., Sperry A.O. Biol. Cell 102:173-189(2010) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH PPP1R42.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U86090 mRNA. Translation: AAB53940.1. BC090841 mRNA. Translation: AAH90841.1. L27153 mRNA. Translation: AAA20133.1. L29223 mRNA. Translation: AAA20132.2.
IPI	IPI00124954.
PIR	I84737.
RefSeq	NP_032474.2. NM_008448.3.
UniGene	Mm.223744.
3D structure databases
ProteinModelPortal	Q61768.
SMR	Q61768. Positions 3-370.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q61768. 3 interactions.
MINT	MINT-236764.
PTM databases
PhosphoSite	Q61768.
Proteomic databases
PaxDb	Q61768.
PRIDE	Q61768.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000025083; ENSMUSP00000025083; ENSMUSG00000006740.
GeneID	16573.
KEGG	mmu:16573.
Organism-specific databases
CTD	3799.
MGI	MGI:1098268. Kif5b.
Phylogenomic databases
eggNOG	COG5059.
GeneTree	ENSGT00690000101739.
HOGENOM	HOG000216718.
HOVERGEN	HBG006210.
InParanoid	Q5BL10.
KO	K10396.
OMA	MMASKPY.
OrthoDB	EOG405S0B.
Enzyme and pathway databases
Reactome	REACT_118324. Disease.
Gene expression databases
ArrayExpress	Q61768.
Bgee	Q61768.
CleanEx	MM_KIF5B.
Genevestigator	Q61768.
GermOnline	ENSMUSG00000006740. Mus musculus.
Family and domain databases
Gene3D	3.40.850.10. 1 hit.
InterPro	IPR019821. Kinesin_motor_CS. IPR001752. Kinesin_motor_dom. [Graphical view]
Pfam	PF00225. Kinesin. 1 hit. [Graphical view]
PRINTS	PR00380. KINESINHEAVY.
SMART	SM00129. KISc. 1 hit. [Graphical view]
PROSITE	PS00411. KINESIN_MOTOR_DOMAIN1. 1 hit. PS50067. KINESIN_MOTOR_DOMAIN2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	290095.
SOURCE	Search...

Entry information

Entry name

KINH_MOUSE

Accession

Primary (citable) accession number: Q61768
Secondary accession number(s): O08711, Q5BL10, Q61580

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	July 27, 2011
Last modified:	April 3, 2013
This is version 106 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program