Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q61768

- KINH_MOUSE

UniProt

Q61768 - KINH_MOUSE

Protein

Kinesin-1 heavy chain

Gene

Kif5b

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 120 (01 Oct 2014)
      Sequence version 3 (27 Jul 2011)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes. May be involved in the mechanisms of growth arrest induced by exposure to DNA-damaging drugs or by cellular senescence.1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi85 – 928ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. microtubule binding Source: HGNC
    3. microtubule motor activity Source: HGNC
    4. protein binding Source: UniProtKB

    GO - Biological processi

    1. cytoplasm organization Source: MGI
    2. metabolic process Source: GOC
    3. microtubule-based process Source: MGI
    4. mitochondrial transport Source: MGI
    5. plus-end-directed vesicle transport along microtubule Source: MGI
    6. positive regulation of establishment of protein localization to plasma membrane Source: Ensembl
    7. positive regulation of potassium ion transport Source: Ensembl
    8. regulation of membrane potential Source: Ensembl
    9. stress granule disassembly Source: BHF-UCL
    10. vesicle transport along microtubule Source: HGNC

    Keywords - Molecular functioni

    Motor protein

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_207837. Insulin processing.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Kinesin-1 heavy chain
    Alternative name(s):
    Conventional kinesin heavy chain
    Ubiquitous kinesin heavy chain
    Short name:
    UKHC
    Gene namesi
    Name:Kif5b
    Synonyms:Khcs, Kns1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 18

    Organism-specific databases

    MGIiMGI:1098268. Kif5b.

    Subcellular locationi

    Cytoplasmcytoskeleton By similarity
    Note: Uniformly distributed between soma and neurites in hippocampal neurons.By similarity

    GO - Cellular componenti

    1. ciliary rootlet Source: MGI
    2. cytoplasm Source: MGI
    3. cytosol Source: Reactome
    4. endocytic vesicle Source: MGI
    5. kinesin complex Source: MGI
    6. membrane-bounded organelle Source: MGI
    7. microtubule Source: UniProtKB-KW
    8. neuron projection Source: MGI
    9. perinuclear region of cytoplasm Source: HGNC
    10. vesicle Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton, Microtubule

    Pathology & Biotechi

    Disruption phenotypei

    Mice are embryonic lethal. They show perinuclear clustering of mitochondria and impaired lysosomal dispersion.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 963962Kinesin-1 heavy chainPRO_0000125352Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity
    Modified residuei933 – 9331PhosphoserineBy similarity
    Modified residuei945 – 9451Phosphoserine1 Publication

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiQ61768.
    PaxDbiQ61768.
    PRIDEiQ61768.

    PTM databases

    PhosphoSiteiQ61768.

    Expressioni

    Gene expression databases

    ArrayExpressiQ61768.
    BgeeiQ61768.
    CleanExiMM_KIF5B.
    GenevestigatoriQ61768.

    Interactioni

    Subunit structurei

    Interacts with SYBU By similarity. Interacts with PLEKHM2 By similarity. Interacts with ECM29 By similarity. Oligomer composed of two heavy chains and two light chains. Interacts with GRIP1. Interacts with JAKMIP1 and PPP1R42.By similarity3 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Dync1i1O884853EBI-776129,EBI-492834
    NudcO356854EBI-776129,EBI-911192

    Protein-protein interaction databases

    BioGridi200946. 8 interactions.
    DIPiDIP-32053N.
    IntActiQ61768. 14 interactions.
    MINTiMINT-236764.

    Structurei

    3D structure databases

    ProteinModelPortaliQ61768.
    SMRiQ61768. Positions 3-370.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini8 – 325318Kinesin motorPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni915 – 96349GlobularAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili329 – 914586Add
    BLAST

    Domaini

    Composed of three structural domains: a large globular N-terminal domain which is responsible for the motor activity of kinesin (it hydrolyzes ATP and binds microtubule), a central alpha-helical coiled coil domain that mediates the heavy chain dimerization; and a small globular C-terminal domain which interacts with other proteins (such as the kinesin light chains), vesicles and membranous organelles.

    Sequence similaritiesi

    Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. Kinesin subfamily.PROSITE-ProRule annotation
    Contains 1 kinesin motor domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiCOG5059.
    GeneTreeiENSGT00730000110208.
    HOGENOMiHOG000216718.
    HOVERGENiHBG006210.
    InParanoidiQ5BL10.
    KOiK10396.
    OMAiDQNQKMV.
    OrthoDBiEOG7T4MJD.
    TreeFamiTF105225.

    Family and domain databases

    Gene3Di3.40.850.10. 1 hit.
    InterProiIPR027640. Kinesin-like_fam.
    IPR019821. Kinesin_motor_CS.
    IPR001752. Kinesin_motor_dom.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PANTHERiPTHR24115. PTHR24115. 1 hit.
    PfamiPF00225. Kinesin. 1 hit.
    [Graphical view]
    PRINTSiPR00380. KINESINHEAVY.
    SMARTiSM00129. KISc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    PROSITEiPS00411. KINESIN_MOTOR_1. 1 hit.
    PS50067. KINESIN_MOTOR_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q61768-1 [UniParc]FASTAAdd to Basket

    « Hide

    MADPAECNIK VMCRFRPLNE SEVNRGDKYV AKFQGEDTVM IASKPYAFDR    50
    VFQSSTSQEQ VYNDCAKKIV KDVLEGYNGT IFAYGQTSSG KTHTMEGKLH 100
    DPEGMGIIPR IVQDIFNYIY SMDENLEFHI KVSYFEIYLD KIRDLLDVSK 150
    TNLSVHEDKN RVPYVKGCTE RFVCSPDEVM DTIDEGKSNR HVAVTNMNEH 200
    SSRSHSIFLI NVKQENTQTE QKLSGKLYLV DLAGSEKVSK TGAEGAVLDE 250
    AKNINKSLSA LGNVISALAE GSTYVPYRDS KMTRILQDSL GGNCRTTIVI 300
    CCSPSSYNES ETKSTLLFGQ RAKTIKNTVC VNVELTAEQW KKKYEKEKEK 350
    NKTLRNTIQW LENELNRWRN GETVPIDEQF DKEKANLEAF TADKDIAITS 400
    DKPAAAVGMA GSFTDAERRK CEEELAKLYK QLDDKDEEIN QQSQLVEKLK 450
    TQMLDQEELL ASTRRDQDNM QAELNRLQAE NDASKEEVKE VLQALEELAV 500
    NYDQKSQEVE DKTKEYELLS DELNQKSATL ASIDAELQKL KEMTNHQKKR 550
    AAEMMASLLK DLAEIGIAVG NNDVKQPEGT GMIDEEFTVA RLYISKMKSE 600
    VKTMVKRCKQ LESTQTESNK KMEENEKELA ACQLRISQHE AKIKSLTEYL 650
    QNVEQKKRQL EESVDSLGEE LVQLRAQEKV HEMEKEHLNK VQTANEVKQA 700
    VEQQIQSHRE THQKQISSLR DEVEAKEKLI TDLQDQNQKM VLEQERLRVE 750
    HERLKATDQE KSRKLHELTV MQDRREQARQ DLKGLEETVA KELQTLHNLR 800
    KLFVQDLATR VKKSAEVDSD DTGGSAAQKQ KISFLENNLE QLTKVHKQLV 850
    RDNADLRCEL PKLEKRLRAT AERVKALESA LKEAKENASR DRKRYQQEVD 900
    RIKEAVRSKN MARRGHSAQI AKPIRPGQHP AASPTHPGTV RGGGSFVQNN 950
    QPVGLRGGGG KQS 963
    Length:963
    Mass (Da):109,551
    Last modified:July 27, 2011 - v3
    Checksum:iC3D3461A0C314901
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti20 – 212ES → AT in AAA20133. (PubMed:8170981)Curated
    Sequence conflicti40 – 401M → V in AAB53940. (PubMed:9112396)Curated
    Sequence conflicti75 – 751E → G in AAA20133. (PubMed:8170981)Curated
    Sequence conflicti191 – 1911H → D in AAA20133. (PubMed:8170981)Curated
    Sequence conflicti255 – 2551N → K in AAA20133. (PubMed:8170981)Curated
    Sequence conflicti396 – 3961I → T in AAA20133. (PubMed:8170981)Curated
    Sequence conflicti403 – 4031P → G in AAB53940. (PubMed:9112396)Curated
    Sequence conflicti451 – 4511T → I in AAA20132. (PubMed:8170981)Curated
    Sequence conflicti520 – 5201S → T in AAB53940. (PubMed:9112396)Curated
    Sequence conflicti523 – 5231L → F in AAB53940. (PubMed:9112396)Curated
    Sequence conflicti653 – 6531V → D in AAB53940. (PubMed:9112396)Curated
    Sequence conflicti664 – 6641V → L in AAB53940. (PubMed:9112396)Curated
    Sequence conflicti744 – 7441Q → T in AAB53940. (PubMed:9112396)Curated
    Sequence conflicti814 – 8141S → R in AAA20133. (PubMed:8170981)Curated
    Sequence conflicti865 – 8651K → F in AAB53940. (PubMed:9112396)Curated
    Sequence conflicti881 – 8811L → R in AAA20133. (PubMed:8170981)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U86090 mRNA. Translation: AAB53940.1.
    BC090841 mRNA. Translation: AAH90841.1.
    L27153 mRNA. Translation: AAA20133.1.
    L29223 mRNA. Translation: AAA20132.2.
    CCDSiCCDS37724.1.
    PIRiI84737.
    RefSeqiNP_032474.2. NM_008448.3.
    UniGeneiMm.223744.

    Genome annotation databases

    EnsembliENSMUST00000025083; ENSMUSP00000025083; ENSMUSG00000006740.
    GeneIDi16573.
    KEGGimmu:16573.
    UCSCiuc008dzf.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U86090 mRNA. Translation: AAB53940.1 .
    BC090841 mRNA. Translation: AAH90841.1 .
    L27153 mRNA. Translation: AAA20133.1 .
    L29223 mRNA. Translation: AAA20132.2 .
    CCDSi CCDS37724.1.
    PIRi I84737.
    RefSeqi NP_032474.2. NM_008448.3.
    UniGenei Mm.223744.

    3D structure databases

    ProteinModelPortali Q61768.
    SMRi Q61768. Positions 3-370.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 200946. 8 interactions.
    DIPi DIP-32053N.
    IntActi Q61768. 14 interactions.
    MINTi MINT-236764.

    PTM databases

    PhosphoSitei Q61768.

    Proteomic databases

    MaxQBi Q61768.
    PaxDbi Q61768.
    PRIDEi Q61768.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000025083 ; ENSMUSP00000025083 ; ENSMUSG00000006740 .
    GeneIDi 16573.
    KEGGi mmu:16573.
    UCSCi uc008dzf.2. mouse.

    Organism-specific databases

    CTDi 3799.
    MGIi MGI:1098268. Kif5b.

    Phylogenomic databases

    eggNOGi COG5059.
    GeneTreei ENSGT00730000110208.
    HOGENOMi HOG000216718.
    HOVERGENi HBG006210.
    InParanoidi Q5BL10.
    KOi K10396.
    OMAi DQNQKMV.
    OrthoDBi EOG7T4MJD.
    TreeFami TF105225.

    Enzyme and pathway databases

    Reactomei REACT_207837. Insulin processing.

    Miscellaneous databases

    NextBioi 290095.
    PROi Q61768.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q61768.
    Bgeei Q61768.
    CleanExi MM_KIF5B.
    Genevestigatori Q61768.

    Family and domain databases

    Gene3Di 3.40.850.10. 1 hit.
    InterProi IPR027640. Kinesin-like_fam.
    IPR019821. Kinesin_motor_CS.
    IPR001752. Kinesin_motor_dom.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    PANTHERi PTHR24115. PTHR24115. 1 hit.
    Pfami PF00225. Kinesin. 1 hit.
    [Graphical view ]
    PRINTSi PR00380. KINESINHEAVY.
    SMARTi SM00129. KISc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    PROSITEi PS00411. KINESIN_MOTOR_1. 1 hit.
    PS50067. KINESIN_MOTOR_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Suppression of the expression of a pancreatic beta-cell form of the kinesin heavy chain by antisense oligonucleotides inhibits insulin secretion from primary cultures of mouse beta-cells."
      Meng Y.X., Wilson G.W., Avery M.C., Varden C.H., Balczon R.
      Endocrinology 138:1979-1987(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Pancreas.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Brain.
    3. "Cloning mammalian genes by expression selection of genetic suppressor elements: association of kinesin with drug resistance and cell immortalization."
      Gudkov A.V., Kazarov A.R., Thimmapaya R., Axenovich S.A., Mazo I.A., Roninson I.B.
      Proc. Natl. Acad. Sci. U.S.A. 91:3744-3748(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-881.
      Strain: BALB/c.
    4. Lubec G., Sunyer B., Chen W.-Q.
      Submitted (JAN-2009) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 132-141 AND 490-505, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: OF1.
      Tissue: Hippocampus.
    5. "Targeted disruption of mouse conventional kinesin heavy chain, kif5B, results in abnormal perinuclear clustering of mitochondria."
      Tanaka Y., Kanai Y., Okada Y., Nonaka S., Takeda S., Harada A., Hirokawa N.
      Cell 93:1147-1158(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE.
    6. "Glutamate-receptor-interacting protein GRIP1 directly steers kinesin to dendrites."
      Setou M., Seog D.-H., Tanaka Y., Kanai Y., Takei Y., Kawagishi M., Hirokawa N.
      Nature 417:83-87(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH GRIP1.
    7. "Marlin-1 and conventional kinesin link GABAB receptors to the cytoskeleton and regulate receptor transport."
      Vidal R.L., Ramirez O.A., Sandoval L., Koenig-Robert R., Haertel S., Couve A.
      Mol. Cell. Neurosci. 35:501-512(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH JAKMIP1.
    8. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-945, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    9. "TLRR (lrrc67) interacts with PP1 and is associated with a cytoskeletal complex in the testis."
      Wang R., Kaul A., Sperry A.O.
      Biol. Cell 102:173-189(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH PPP1R42.

    Entry informationi

    Entry nameiKINH_MOUSE
    AccessioniPrimary (citable) accession number: Q61768
    Secondary accession number(s): O08711, Q5BL10, Q61580
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 120 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3