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Q61768

- KINH_MOUSE

UniProt

Q61768 - KINH_MOUSE

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Protein

Kinesin-1 heavy chain

Gene

Kif5b

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes. May be involved in the mechanisms of growth arrest induced by exposure to DNA-damaging drugs or by cellular senescence.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi85 – 928ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATPase activity Source: RefGenome
  2. ATP binding Source: UniProtKB-KW
  3. microtubule binding Source: HGNC
  4. microtubule motor activity Source: HGNC
  5. plus-end-directed microtubule motor activity Source: RefGenome

GO - Biological processi

  1. ATP catabolic process Source: GOC
  2. axon guidance Source: RefGenome
  3. cytoplasm organization Source: MGI
  4. metabolic process Source: GOC
  5. microtubule-based process Source: MGI
  6. mitochondrial transport Source: MGI
  7. plus-end-directed vesicle transport along microtubule Source: MGI
  8. positive regulation of establishment of protein localization to plasma membrane Source: Ensembl
  9. positive regulation of potassium ion transport Source: Ensembl
  10. positive regulation of synaptic transmission, GABAergic Source: UniProtKB
  11. protein localization Source: RefGenome
  12. regulation of membrane potential Source: Ensembl
  13. stress granule disassembly Source: BHF-UCL
  14. vesicle transport along microtubule Source: HGNC
Complete GO annotation...

Keywords - Molecular functioni

Motor protein

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_207837. Insulin processing.

Names & Taxonomyi

Protein namesi
Recommended name:
Kinesin-1 heavy chain
Alternative name(s):
Conventional kinesin heavy chain
Ubiquitous kinesin heavy chain
Short name:
UKHC
Gene namesi
Name:Kif5b
Synonyms:Khcs, Kns1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 18

Organism-specific databases

MGIiMGI:1098268. Kif5b.

Subcellular locationi

Cytoplasmcytoskeleton By similarity
Note: Uniformly distributed between soma and neurites in hippocampal neurons.By similarity

GO - Cellular componenti

  1. ciliary rootlet Source: MGI
  2. cytoplasm Source: MGI
  3. cytosol Source: Reactome
  4. endocytic vesicle Source: MGI
  5. kinesin complex Source: RefGenome
  6. membrane-bounded organelle Source: MGI
  7. microtubule Source: UniProtKB-KW
  8. microtubule organizing center Source: Ensembl
  9. neuron projection Source: MGI
  10. perinuclear region of cytoplasm Source: HGNC
  11. vesicle Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

Pathology & Biotechi

Disruption phenotypei

Mice are embryonic lethal. They show perinuclear clustering of mitochondria and impaired lysosomal dispersion.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 963962Kinesin-1 heavy chainPRO_0000125352Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei933 – 9331PhosphoserineBy similarity
Modified residuei945 – 9451Phosphoserine1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ61768.
PaxDbiQ61768.
PRIDEiQ61768.

PTM databases

PhosphoSiteiQ61768.

Expressioni

Gene expression databases

BgeeiQ61768.
CleanExiMM_KIF5B.
ExpressionAtlasiQ61768. baseline and differential.
GenevestigatoriQ61768.

Interactioni

Subunit structurei

Interacts with SYBU (By similarity). Interacts with PLEKHM2 (By similarity). Interacts with ECM29 (By similarity). Oligomer composed of two heavy chains and two light chains. Interacts with GRIP1. Interacts with JAKMIP1 and PPP1R42.By similarity3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Dync1i1O884853EBI-776129,EBI-492834
NudcO356854EBI-776129,EBI-911192

Protein-protein interaction databases

BioGridi200946. 8 interactions.
DIPiDIP-32053N.
IntActiQ61768. 15 interactions.
MINTiMINT-236764.

Structurei

3D structure databases

ProteinModelPortaliQ61768.
SMRiQ61768. Positions 3-370, 725-762.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini8 – 325318Kinesin motorPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni915 – 96349GlobularAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili329 – 914586Add
BLAST

Domaini

Composed of three structural domains: a large globular N-terminal domain which is responsible for the motor activity of kinesin (it hydrolyzes ATP and binds microtubule), a central alpha-helical coiled coil domain that mediates the heavy chain dimerization; and a small globular C-terminal domain which interacts with other proteins (such as the kinesin light chains), vesicles and membranous organelles.

Sequence similaritiesi

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. Kinesin subfamily.PROSITE-ProRule annotation
Contains 1 kinesin motor domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiCOG5059.
GeneTreeiENSGT00760000118872.
HOGENOMiHOG000216718.
HOVERGENiHBG006210.
InParanoidiQ61768.
KOiK10396.
OMAiDQNQKMV.
OrthoDBiEOG7T4MJD.
TreeFamiTF105225.

Family and domain databases

Gene3Di3.40.850.10. 1 hit.
InterProiIPR027640. Kinesin-like_fam.
IPR019821. Kinesin_motor_CS.
IPR001752. Kinesin_motor_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR24115. PTHR24115. 1 hit.
PfamiPF00225. Kinesin. 1 hit.
[Graphical view]
PRINTSiPR00380. KINESINHEAVY.
SMARTiSM00129. KISc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00411. KINESIN_MOTOR_1. 1 hit.
PS50067. KINESIN_MOTOR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q61768-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MADPAECNIK VMCRFRPLNE SEVNRGDKYV AKFQGEDTVM IASKPYAFDR
60 70 80 90 100
VFQSSTSQEQ VYNDCAKKIV KDVLEGYNGT IFAYGQTSSG KTHTMEGKLH
110 120 130 140 150
DPEGMGIIPR IVQDIFNYIY SMDENLEFHI KVSYFEIYLD KIRDLLDVSK
160 170 180 190 200
TNLSVHEDKN RVPYVKGCTE RFVCSPDEVM DTIDEGKSNR HVAVTNMNEH
210 220 230 240 250
SSRSHSIFLI NVKQENTQTE QKLSGKLYLV DLAGSEKVSK TGAEGAVLDE
260 270 280 290 300
AKNINKSLSA LGNVISALAE GSTYVPYRDS KMTRILQDSL GGNCRTTIVI
310 320 330 340 350
CCSPSSYNES ETKSTLLFGQ RAKTIKNTVC VNVELTAEQW KKKYEKEKEK
360 370 380 390 400
NKTLRNTIQW LENELNRWRN GETVPIDEQF DKEKANLEAF TADKDIAITS
410 420 430 440 450
DKPAAAVGMA GSFTDAERRK CEEELAKLYK QLDDKDEEIN QQSQLVEKLK
460 470 480 490 500
TQMLDQEELL ASTRRDQDNM QAELNRLQAE NDASKEEVKE VLQALEELAV
510 520 530 540 550
NYDQKSQEVE DKTKEYELLS DELNQKSATL ASIDAELQKL KEMTNHQKKR
560 570 580 590 600
AAEMMASLLK DLAEIGIAVG NNDVKQPEGT GMIDEEFTVA RLYISKMKSE
610 620 630 640 650
VKTMVKRCKQ LESTQTESNK KMEENEKELA ACQLRISQHE AKIKSLTEYL
660 670 680 690 700
QNVEQKKRQL EESVDSLGEE LVQLRAQEKV HEMEKEHLNK VQTANEVKQA
710 720 730 740 750
VEQQIQSHRE THQKQISSLR DEVEAKEKLI TDLQDQNQKM VLEQERLRVE
760 770 780 790 800
HERLKATDQE KSRKLHELTV MQDRREQARQ DLKGLEETVA KELQTLHNLR
810 820 830 840 850
KLFVQDLATR VKKSAEVDSD DTGGSAAQKQ KISFLENNLE QLTKVHKQLV
860 870 880 890 900
RDNADLRCEL PKLEKRLRAT AERVKALESA LKEAKENASR DRKRYQQEVD
910 920 930 940 950
RIKEAVRSKN MARRGHSAQI AKPIRPGQHP AASPTHPGTV RGGGSFVQNN
960
QPVGLRGGGG KQS
Length:963
Mass (Da):109,551
Last modified:July 27, 2011 - v3
Checksum:iC3D3461A0C314901
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti20 – 212ES → AT in AAA20133. (PubMed:8170981)Curated
Sequence conflicti40 – 401M → V in AAB53940. (PubMed:9112396)Curated
Sequence conflicti75 – 751E → G in AAA20133. (PubMed:8170981)Curated
Sequence conflicti191 – 1911H → D in AAA20133. (PubMed:8170981)Curated
Sequence conflicti255 – 2551N → K in AAA20133. (PubMed:8170981)Curated
Sequence conflicti396 – 3961I → T in AAA20133. (PubMed:8170981)Curated
Sequence conflicti403 – 4031P → G in AAB53940. (PubMed:9112396)Curated
Sequence conflicti451 – 4511T → I in AAA20132. (PubMed:8170981)Curated
Sequence conflicti520 – 5201S → T in AAB53940. (PubMed:9112396)Curated
Sequence conflicti523 – 5231L → F in AAB53940. (PubMed:9112396)Curated
Sequence conflicti653 – 6531V → D in AAB53940. (PubMed:9112396)Curated
Sequence conflicti664 – 6641V → L in AAB53940. (PubMed:9112396)Curated
Sequence conflicti744 – 7441Q → T in AAB53940. (PubMed:9112396)Curated
Sequence conflicti814 – 8141S → R in AAA20133. (PubMed:8170981)Curated
Sequence conflicti865 – 8651K → F in AAB53940. (PubMed:9112396)Curated
Sequence conflicti881 – 8811L → R in AAA20133. (PubMed:8170981)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U86090 mRNA. Translation: AAB53940.1.
BC090841 mRNA. Translation: AAH90841.1.
L27153 mRNA. Translation: AAA20133.1.
L29223 mRNA. Translation: AAA20132.2.
CCDSiCCDS37724.1.
PIRiI84737.
RefSeqiNP_032474.2. NM_008448.3.
UniGeneiMm.223744.

Genome annotation databases

EnsembliENSMUST00000025083; ENSMUSP00000025083; ENSMUSG00000006740.
GeneIDi16573.
KEGGimmu:16573.
UCSCiuc008dzf.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U86090 mRNA. Translation: AAB53940.1 .
BC090841 mRNA. Translation: AAH90841.1 .
L27153 mRNA. Translation: AAA20133.1 .
L29223 mRNA. Translation: AAA20132.2 .
CCDSi CCDS37724.1.
PIRi I84737.
RefSeqi NP_032474.2. NM_008448.3.
UniGenei Mm.223744.

3D structure databases

ProteinModelPortali Q61768.
SMRi Q61768. Positions 3-370, 725-762.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 200946. 8 interactions.
DIPi DIP-32053N.
IntActi Q61768. 15 interactions.
MINTi MINT-236764.

PTM databases

PhosphoSitei Q61768.

Proteomic databases

MaxQBi Q61768.
PaxDbi Q61768.
PRIDEi Q61768.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000025083 ; ENSMUSP00000025083 ; ENSMUSG00000006740 .
GeneIDi 16573.
KEGGi mmu:16573.
UCSCi uc008dzf.2. mouse.

Organism-specific databases

CTDi 3799.
MGIi MGI:1098268. Kif5b.

Phylogenomic databases

eggNOGi COG5059.
GeneTreei ENSGT00760000118872.
HOGENOMi HOG000216718.
HOVERGENi HBG006210.
InParanoidi Q61768.
KOi K10396.
OMAi DQNQKMV.
OrthoDBi EOG7T4MJD.
TreeFami TF105225.

Enzyme and pathway databases

Reactomei REACT_207837. Insulin processing.

Miscellaneous databases

NextBioi 290095.
PROi Q61768.
SOURCEi Search...

Gene expression databases

Bgeei Q61768.
CleanExi MM_KIF5B.
ExpressionAtlasi Q61768. baseline and differential.
Genevestigatori Q61768.

Family and domain databases

Gene3Di 3.40.850.10. 1 hit.
InterProi IPR027640. Kinesin-like_fam.
IPR019821. Kinesin_motor_CS.
IPR001752. Kinesin_motor_dom.
IPR027417. P-loop_NTPase.
[Graphical view ]
PANTHERi PTHR24115. PTHR24115. 1 hit.
Pfami PF00225. Kinesin. 1 hit.
[Graphical view ]
PRINTSi PR00380. KINESINHEAVY.
SMARTi SM00129. KISc. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
PROSITEi PS00411. KINESIN_MOTOR_1. 1 hit.
PS50067. KINESIN_MOTOR_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Suppression of the expression of a pancreatic beta-cell form of the kinesin heavy chain by antisense oligonucleotides inhibits insulin secretion from primary cultures of mouse beta-cells."
    Meng Y.X., Wilson G.W., Avery M.C., Varden C.H., Balczon R.
    Endocrinology 138:1979-1987(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Pancreas.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain.
  3. "Cloning mammalian genes by expression selection of genetic suppressor elements: association of kinesin with drug resistance and cell immortalization."
    Gudkov A.V., Kazarov A.R., Thimmapaya R., Axenovich S.A., Mazo I.A., Roninson I.B.
    Proc. Natl. Acad. Sci. U.S.A. 91:3744-3748(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-881.
    Strain: BALB/c.
  4. Lubec G., Sunyer B., Chen W.-Q.
    Submitted (JAN-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 132-141 AND 490-505, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: OF1.
    Tissue: Hippocampus.
  5. "Targeted disruption of mouse conventional kinesin heavy chain, kif5B, results in abnormal perinuclear clustering of mitochondria."
    Tanaka Y., Kanai Y., Okada Y., Nonaka S., Takeda S., Harada A., Hirokawa N.
    Cell 93:1147-1158(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  6. "Glutamate-receptor-interacting protein GRIP1 directly steers kinesin to dendrites."
    Setou M., Seog D.-H., Tanaka Y., Kanai Y., Takei Y., Kawagishi M., Hirokawa N.
    Nature 417:83-87(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GRIP1.
  7. "Marlin-1 and conventional kinesin link GABAB receptors to the cytoskeleton and regulate receptor transport."
    Vidal R.L., Ramirez O.A., Sandoval L., Koenig-Robert R., Haertel S., Couve A.
    Mol. Cell. Neurosci. 35:501-512(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH JAKMIP1.
  8. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-945, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  9. "TLRR (lrrc67) interacts with PP1 and is associated with a cytoskeletal complex in the testis."
    Wang R., Kaul A., Sperry A.O.
    Biol. Cell 102:173-189(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PPP1R42.

Entry informationi

Entry nameiKINH_MOUSE
AccessioniPrimary (citable) accession number: Q61768
Secondary accession number(s): O08711, Q5BL10, Q61580
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 27, 2011
Last modified: October 29, 2014
This is version 121 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3