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Protein

Kinesin-1 heavy chain

Gene

Kif5b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes. May be involved in the mechanisms of growth arrest induced by exposure to DNA-damaging drugs or by cellular senescence. Can induce formation of neurite-like membrane protrusions in non-neuronal cells in a ZFYVE27-dependent manner (PubMed:21976701).2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi85 – 92ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Motor protein

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-264876. Insulin processing.

Names & Taxonomyi

Protein namesi
Recommended name:
Kinesin-1 heavy chain
Alternative name(s):
Conventional kinesin heavy chain
Ubiquitous kinesin heavy chain
Short name:
UKHC
Gene namesi
Name:Kif5b
Synonyms:Khcs, Kns1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:1098268. Kif5b.

Subcellular locationi

  • Cytoplasmcytoskeleton By similarity

  • Note: Uniformly distributed between soma and neurites in hippocampal neurons.By similarity

GO - Cellular componenti

  • axonal growth cone Source: Ensembl
  • cell-cell adherens junction Source: MGI
  • ciliary rootlet Source: MGI
  • cytoplasm Source: MGI
  • cytosol Source: Reactome
  • endocytic vesicle Source: MGI
  • kinesin complex Source: MGI
  • membrane Source: MGI
  • membrane-bounded organelle Source: MGI
  • microtubule Source: UniProtKB-KW
  • microtubule organizing center Source: MGI
  • neuron projection Source: MGI
  • perinuclear region of cytoplasm Source: HGNC
  • vesicle Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

Pathology & Biotechi

Disruption phenotypei

Mice are embryonic lethal. They show perinuclear clustering of mitochondria and impaired lysosomal dispersion.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001253522 – 963Kinesin-1 heavy chainAdd BLAST962

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei933PhosphoserineBy similarity1
Modified residuei945PhosphoserineCombined sources1
Modified residuei956Omega-N-methylarginineCombined sources1

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

EPDiQ61768.
MaxQBiQ61768.
PaxDbiQ61768.
PeptideAtlasiQ61768.
PRIDEiQ61768.

PTM databases

iPTMnetiQ61768.
PhosphoSitePlusiQ61768.

Expressioni

Gene expression databases

BgeeiENSMUSG00000006740.
CleanExiMM_KIF5B.
ExpressionAtlasiQ61768. baseline and differential.
GenevisibleiQ61768. MM.

Interactioni

Subunit structurei

Interacts with SYBU (By similarity). Interacts with PLEKHM2 (By similarity). Interacts with ECM29 (By similarity). Oligomer composed of two heavy chains and two light chains. Interacts with GRIP1 (PubMed:11986669). Interacts with JAKMIP1 (PubMed:17532644). Interacts with PPP1R42 (PubMed:19886865). Interacts with ZFYVE27 (PubMed:21976701).By similarity4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Dync1i1O884853EBI-776129,EBI-492834
NudcO356854EBI-776129,EBI-911192

GO - Molecular functioni

Protein-protein interaction databases

BioGridi200946. 21 interactors.
DIPiDIP-32053N.
IntActiQ61768. 30 interactors.
MINTiMINT-236764.
STRINGi10090.ENSMUSP00000025083.

Structurei

3D structure databases

ProteinModelPortaliQ61768.
SMRiQ61768.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 325Kinesin motorPROSITE-ProRule annotationAdd BLAST318

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni915 – 963GlobularAdd BLAST49

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili329 – 914Add BLAST586

Domaini

Composed of three structural domains: a large globular N-terminal domain which is responsible for the motor activity of kinesin (it hydrolyzes ATP and binds microtubule), a central alpha-helical coiled coil domain that mediates the heavy chain dimerization; and a small globular C-terminal domain which interacts with other proteins (such as the kinesin light chains), vesicles and membranous organelles.

Sequence similaritiesi

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. Kinesin subfamily.PROSITE-ProRule annotation
Contains 1 kinesin motor domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0240. Eukaryota.
COG5059. LUCA.
GeneTreeiENSGT00860000133684.
HOGENOMiHOG000216718.
HOVERGENiHBG006210.
InParanoidiQ61768.
KOiK10396.
OMAiLAECNIK.
OrthoDBiEOG091G02A2.
TreeFamiTF105225.

Family and domain databases

Gene3Di3.40.850.10. 1 hit.
InterProiIPR027640. Kinesin-like_fam.
IPR019821. Kinesin_motor_CS.
IPR001752. Kinesin_motor_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR24115. PTHR24115. 1 hit.
PfamiPF00225. Kinesin. 1 hit.
[Graphical view]
PRINTSiPR00380. KINESINHEAVY.
SMARTiSM00129. KISc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00411. KINESIN_MOTOR_1. 1 hit.
PS50067. KINESIN_MOTOR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q61768-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADPAECNIK VMCRFRPLNE SEVNRGDKYV AKFQGEDTVM IASKPYAFDR
60 70 80 90 100
VFQSSTSQEQ VYNDCAKKIV KDVLEGYNGT IFAYGQTSSG KTHTMEGKLH
110 120 130 140 150
DPEGMGIIPR IVQDIFNYIY SMDENLEFHI KVSYFEIYLD KIRDLLDVSK
160 170 180 190 200
TNLSVHEDKN RVPYVKGCTE RFVCSPDEVM DTIDEGKSNR HVAVTNMNEH
210 220 230 240 250
SSRSHSIFLI NVKQENTQTE QKLSGKLYLV DLAGSEKVSK TGAEGAVLDE
260 270 280 290 300
AKNINKSLSA LGNVISALAE GSTYVPYRDS KMTRILQDSL GGNCRTTIVI
310 320 330 340 350
CCSPSSYNES ETKSTLLFGQ RAKTIKNTVC VNVELTAEQW KKKYEKEKEK
360 370 380 390 400
NKTLRNTIQW LENELNRWRN GETVPIDEQF DKEKANLEAF TADKDIAITS
410 420 430 440 450
DKPAAAVGMA GSFTDAERRK CEEELAKLYK QLDDKDEEIN QQSQLVEKLK
460 470 480 490 500
TQMLDQEELL ASTRRDQDNM QAELNRLQAE NDASKEEVKE VLQALEELAV
510 520 530 540 550
NYDQKSQEVE DKTKEYELLS DELNQKSATL ASIDAELQKL KEMTNHQKKR
560 570 580 590 600
AAEMMASLLK DLAEIGIAVG NNDVKQPEGT GMIDEEFTVA RLYISKMKSE
610 620 630 640 650
VKTMVKRCKQ LESTQTESNK KMEENEKELA ACQLRISQHE AKIKSLTEYL
660 670 680 690 700
QNVEQKKRQL EESVDSLGEE LVQLRAQEKV HEMEKEHLNK VQTANEVKQA
710 720 730 740 750
VEQQIQSHRE THQKQISSLR DEVEAKEKLI TDLQDQNQKM VLEQERLRVE
760 770 780 790 800
HERLKATDQE KSRKLHELTV MQDRREQARQ DLKGLEETVA KELQTLHNLR
810 820 830 840 850
KLFVQDLATR VKKSAEVDSD DTGGSAAQKQ KISFLENNLE QLTKVHKQLV
860 870 880 890 900
RDNADLRCEL PKLEKRLRAT AERVKALESA LKEAKENASR DRKRYQQEVD
910 920 930 940 950
RIKEAVRSKN MARRGHSAQI AKPIRPGQHP AASPTHPGTV RGGGSFVQNN
960
QPVGLRGGGG KQS
Length:963
Mass (Da):109,551
Last modified:July 27, 2011 - v3
Checksum:iC3D3461A0C314901
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti20 – 21ES → AT in AAA20133 (PubMed:8170981).Curated2
Sequence conflicti40M → V in AAB53940 (PubMed:9112396).Curated1
Sequence conflicti75E → G in AAA20133 (PubMed:8170981).Curated1
Sequence conflicti191H → D in AAA20133 (PubMed:8170981).Curated1
Sequence conflicti255N → K in AAA20133 (PubMed:8170981).Curated1
Sequence conflicti396I → T in AAA20133 (PubMed:8170981).Curated1
Sequence conflicti403P → G in AAB53940 (PubMed:9112396).Curated1
Sequence conflicti451T → I in AAA20132 (PubMed:8170981).Curated1
Sequence conflicti520S → T in AAB53940 (PubMed:9112396).Curated1
Sequence conflicti523L → F in AAB53940 (PubMed:9112396).Curated1
Sequence conflicti653V → D in AAB53940 (PubMed:9112396).Curated1
Sequence conflicti664V → L in AAB53940 (PubMed:9112396).Curated1
Sequence conflicti744Q → T in AAB53940 (PubMed:9112396).Curated1
Sequence conflicti814S → R in AAA20133 (PubMed:8170981).Curated1
Sequence conflicti865K → F in AAB53940 (PubMed:9112396).Curated1
Sequence conflicti881L → R in AAA20133 (PubMed:8170981).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U86090 mRNA. Translation: AAB53940.1.
BC090841 mRNA. Translation: AAH90841.1.
L27153 mRNA. Translation: AAA20133.1.
L29223 mRNA. Translation: AAA20132.2.
CCDSiCCDS37724.1.
PIRiI84737.
RefSeqiNP_032474.2. NM_008448.3.
UniGeneiMm.223744.

Genome annotation databases

EnsembliENSMUST00000025083; ENSMUSP00000025083; ENSMUSG00000006740.
GeneIDi16573.
KEGGimmu:16573.
UCSCiuc008dzf.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U86090 mRNA. Translation: AAB53940.1.
BC090841 mRNA. Translation: AAH90841.1.
L27153 mRNA. Translation: AAA20133.1.
L29223 mRNA. Translation: AAA20132.2.
CCDSiCCDS37724.1.
PIRiI84737.
RefSeqiNP_032474.2. NM_008448.3.
UniGeneiMm.223744.

3D structure databases

ProteinModelPortaliQ61768.
SMRiQ61768.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200946. 21 interactors.
DIPiDIP-32053N.
IntActiQ61768. 30 interactors.
MINTiMINT-236764.
STRINGi10090.ENSMUSP00000025083.

PTM databases

iPTMnetiQ61768.
PhosphoSitePlusiQ61768.

Proteomic databases

EPDiQ61768.
MaxQBiQ61768.
PaxDbiQ61768.
PeptideAtlasiQ61768.
PRIDEiQ61768.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000025083; ENSMUSP00000025083; ENSMUSG00000006740.
GeneIDi16573.
KEGGimmu:16573.
UCSCiuc008dzf.2. mouse.

Organism-specific databases

CTDi3799.
MGIiMGI:1098268. Kif5b.

Phylogenomic databases

eggNOGiKOG0240. Eukaryota.
COG5059. LUCA.
GeneTreeiENSGT00860000133684.
HOGENOMiHOG000216718.
HOVERGENiHBG006210.
InParanoidiQ61768.
KOiK10396.
OMAiLAECNIK.
OrthoDBiEOG091G02A2.
TreeFamiTF105225.

Enzyme and pathway databases

ReactomeiR-MMU-264876. Insulin processing.

Miscellaneous databases

ChiTaRSiKif5b. mouse.
PROiQ61768.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000006740.
CleanExiMM_KIF5B.
ExpressionAtlasiQ61768. baseline and differential.
GenevisibleiQ61768. MM.

Family and domain databases

Gene3Di3.40.850.10. 1 hit.
InterProiIPR027640. Kinesin-like_fam.
IPR019821. Kinesin_motor_CS.
IPR001752. Kinesin_motor_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR24115. PTHR24115. 1 hit.
PfamiPF00225. Kinesin. 1 hit.
[Graphical view]
PRINTSiPR00380. KINESINHEAVY.
SMARTiSM00129. KISc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00411. KINESIN_MOTOR_1. 1 hit.
PS50067. KINESIN_MOTOR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKINH_MOUSE
AccessioniPrimary (citable) accession number: Q61768
Secondary accession number(s): O08711, Q5BL10, Q61580
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 27, 2011
Last modified: November 30, 2016
This is version 143 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.