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Q61768

- KINH_MOUSE

UniProt

Q61768 - KINH_MOUSE

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Protein

Kinesin-1 heavy chain

Gene
Kif5b, Khcs, Kns1
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes. May be involved in the mechanisms of growth arrest induced by exposure to DNA-damaging drugs or by cellular senescence.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi85 – 928ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. microtubule binding Source: HGNC
  3. microtubule motor activity Source: HGNC
  4. protein binding Source: UniProtKB

GO - Biological processi

  1. cytoplasm organization Source: MGI
  2. metabolic process Source: GOC
  3. microtubule-based process Source: MGI
  4. mitochondrial transport Source: MGI
  5. plus-end-directed vesicle transport along microtubule Source: MGI
  6. positive regulation of establishment of protein localization to plasma membrane Source: Ensembl
  7. positive regulation of potassium ion transport Source: Ensembl
  8. regulation of membrane potential Source: Ensembl
  9. stress granule disassembly Source: BHF-UCL
  10. vesicle transport along microtubule Source: HGNC
Complete GO annotation...

Keywords - Molecular functioni

Motor protein

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_207837. Insulin processing.

Names & Taxonomyi

Protein namesi
Recommended name:
Kinesin-1 heavy chain
Alternative name(s):
Conventional kinesin heavy chain
Ubiquitous kinesin heavy chain
Short name:
UKHC
Gene namesi
Name:Kif5b
Synonyms:Khcs, Kns1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 18

Organism-specific databases

MGIiMGI:1098268. Kif5b.

Subcellular locationi

Cytoplasmcytoskeleton By similarity
Note: Uniformly distributed between soma and neurites in hippocampal neurons By similarity.

GO - Cellular componenti

  1. ciliary rootlet Source: MGI
  2. cytoplasm Source: MGI
  3. cytosol Source: Reactome
  4. endocytic vesicle Source: MGI
  5. kinesin complex Source: MGI
  6. membrane-bounded organelle Source: MGI
  7. microtubule Source: UniProtKB-KW
  8. neuron projection Source: MGI
  9. perinuclear region of cytoplasm Source: HGNC
  10. vesicle Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

Pathology & Biotechi

Disruption phenotypei

Mice are embryonic lethal. They show perinuclear clustering of mitochondria and impaired lysosomal dispersion.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 963962Kinesin-1 heavy chainPRO_0000125352Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine By similarity
Modified residuei933 – 9331Phosphoserine By similarity
Modified residuei945 – 9451Phosphoserine1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ61768.
PaxDbiQ61768.
PRIDEiQ61768.

PTM databases

PhosphoSiteiQ61768.

Expressioni

Gene expression databases

ArrayExpressiQ61768.
BgeeiQ61768.
CleanExiMM_KIF5B.
GenevestigatoriQ61768.

Interactioni

Subunit structurei

Interacts with SYBU By similarity. Interacts with PLEKHM2 By similarity. Interacts with ECM29 By similarity. Oligomer composed of two heavy chains and two light chains. Interacts with GRIP1. Interacts with JAKMIP1 and PPP1R42.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Dync1i1O884853EBI-776129,EBI-492834
NudcO356854EBI-776129,EBI-911192

Protein-protein interaction databases

BioGridi200946. 8 interactions.
IntActiQ61768. 13 interactions.
MINTiMINT-236764.

Structurei

3D structure databases

ProteinModelPortaliQ61768.
SMRiQ61768. Positions 3-370.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini8 – 325318Kinesin motorAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni915 – 96349GlobularAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili329 – 914586Add
BLAST

Domaini

Composed of three structural domains: a large globular N-terminal domain which is responsible for the motor activity of kinesin (it hydrolyzes ATP and binds microtubule), a central alpha-helical coiled coil domain that mediates the heavy chain dimerization; and a small globular C-terminal domain which interacts with other proteins (such as the kinesin light chains), vesicles and membranous organelles.

Sequence similaritiesi

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiCOG5059.
GeneTreeiENSGT00730000110208.
HOGENOMiHOG000216718.
HOVERGENiHBG006210.
InParanoidiQ5BL10.
KOiK10396.
OMAiDQNQKMV.
OrthoDBiEOG7T4MJD.
TreeFamiTF105225.

Family and domain databases

Gene3Di3.40.850.10. 1 hit.
InterProiIPR027640. Kinesin-like_fam.
IPR019821. Kinesin_motor_CS.
IPR001752. Kinesin_motor_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR24115. PTHR24115. 1 hit.
PfamiPF00225. Kinesin. 1 hit.
[Graphical view]
PRINTSiPR00380. KINESINHEAVY.
SMARTiSM00129. KISc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00411. KINESIN_MOTOR_1. 1 hit.
PS50067. KINESIN_MOTOR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q61768-1 [UniParc]FASTAAdd to Basket

« Hide

MADPAECNIK VMCRFRPLNE SEVNRGDKYV AKFQGEDTVM IASKPYAFDR    50
VFQSSTSQEQ VYNDCAKKIV KDVLEGYNGT IFAYGQTSSG KTHTMEGKLH 100
DPEGMGIIPR IVQDIFNYIY SMDENLEFHI KVSYFEIYLD KIRDLLDVSK 150
TNLSVHEDKN RVPYVKGCTE RFVCSPDEVM DTIDEGKSNR HVAVTNMNEH 200
SSRSHSIFLI NVKQENTQTE QKLSGKLYLV DLAGSEKVSK TGAEGAVLDE 250
AKNINKSLSA LGNVISALAE GSTYVPYRDS KMTRILQDSL GGNCRTTIVI 300
CCSPSSYNES ETKSTLLFGQ RAKTIKNTVC VNVELTAEQW KKKYEKEKEK 350
NKTLRNTIQW LENELNRWRN GETVPIDEQF DKEKANLEAF TADKDIAITS 400
DKPAAAVGMA GSFTDAERRK CEEELAKLYK QLDDKDEEIN QQSQLVEKLK 450
TQMLDQEELL ASTRRDQDNM QAELNRLQAE NDASKEEVKE VLQALEELAV 500
NYDQKSQEVE DKTKEYELLS DELNQKSATL ASIDAELQKL KEMTNHQKKR 550
AAEMMASLLK DLAEIGIAVG NNDVKQPEGT GMIDEEFTVA RLYISKMKSE 600
VKTMVKRCKQ LESTQTESNK KMEENEKELA ACQLRISQHE AKIKSLTEYL 650
QNVEQKKRQL EESVDSLGEE LVQLRAQEKV HEMEKEHLNK VQTANEVKQA 700
VEQQIQSHRE THQKQISSLR DEVEAKEKLI TDLQDQNQKM VLEQERLRVE 750
HERLKATDQE KSRKLHELTV MQDRREQARQ DLKGLEETVA KELQTLHNLR 800
KLFVQDLATR VKKSAEVDSD DTGGSAAQKQ KISFLENNLE QLTKVHKQLV 850
RDNADLRCEL PKLEKRLRAT AERVKALESA LKEAKENASR DRKRYQQEVD 900
RIKEAVRSKN MARRGHSAQI AKPIRPGQHP AASPTHPGTV RGGGSFVQNN 950
QPVGLRGGGG KQS 963
Length:963
Mass (Da):109,551
Last modified:July 27, 2011 - v3
Checksum:iC3D3461A0C314901
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti20 – 212ES → AT in AAA20133. 1 Publication
Sequence conflicti40 – 401M → V in AAB53940. 1 Publication
Sequence conflicti75 – 751E → G in AAA20133. 1 Publication
Sequence conflicti191 – 1911H → D in AAA20133. 1 Publication
Sequence conflicti255 – 2551N → K in AAA20133. 1 Publication
Sequence conflicti396 – 3961I → T in AAA20133. 1 Publication
Sequence conflicti403 – 4031P → G in AAB53940. 1 Publication
Sequence conflicti451 – 4511T → I in AAA20132. 1 Publication
Sequence conflicti520 – 5201S → T in AAB53940. 1 Publication
Sequence conflicti523 – 5231L → F in AAB53940. 1 Publication
Sequence conflicti653 – 6531V → D in AAB53940. 1 Publication
Sequence conflicti664 – 6641V → L in AAB53940. 1 Publication
Sequence conflicti744 – 7441Q → T in AAB53940. 1 Publication
Sequence conflicti814 – 8141S → R in AAA20133. 1 Publication
Sequence conflicti865 – 8651K → F in AAB53940. 1 Publication
Sequence conflicti881 – 8811L → R in AAA20133. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U86090 mRNA. Translation: AAB53940.1.
BC090841 mRNA. Translation: AAH90841.1.
L27153 mRNA. Translation: AAA20133.1.
L29223 mRNA. Translation: AAA20132.2.
CCDSiCCDS37724.1.
PIRiI84737.
RefSeqiNP_032474.2. NM_008448.3.
UniGeneiMm.223744.

Genome annotation databases

EnsembliENSMUST00000025083; ENSMUSP00000025083; ENSMUSG00000006740.
GeneIDi16573.
KEGGimmu:16573.
UCSCiuc008dzf.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U86090 mRNA. Translation: AAB53940.1 .
BC090841 mRNA. Translation: AAH90841.1 .
L27153 mRNA. Translation: AAA20133.1 .
L29223 mRNA. Translation: AAA20132.2 .
CCDSi CCDS37724.1.
PIRi I84737.
RefSeqi NP_032474.2. NM_008448.3.
UniGenei Mm.223744.

3D structure databases

ProteinModelPortali Q61768.
SMRi Q61768. Positions 3-370.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 200946. 8 interactions.
IntActi Q61768. 13 interactions.
MINTi MINT-236764.

PTM databases

PhosphoSitei Q61768.

Proteomic databases

MaxQBi Q61768.
PaxDbi Q61768.
PRIDEi Q61768.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000025083 ; ENSMUSP00000025083 ; ENSMUSG00000006740 .
GeneIDi 16573.
KEGGi mmu:16573.
UCSCi uc008dzf.2. mouse.

Organism-specific databases

CTDi 3799.
MGIi MGI:1098268. Kif5b.

Phylogenomic databases

eggNOGi COG5059.
GeneTreei ENSGT00730000110208.
HOGENOMi HOG000216718.
HOVERGENi HBG006210.
InParanoidi Q5BL10.
KOi K10396.
OMAi DQNQKMV.
OrthoDBi EOG7T4MJD.
TreeFami TF105225.

Enzyme and pathway databases

Reactomei REACT_207837. Insulin processing.

Miscellaneous databases

NextBioi 290095.
PROi Q61768.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q61768.
Bgeei Q61768.
CleanExi MM_KIF5B.
Genevestigatori Q61768.

Family and domain databases

Gene3Di 3.40.850.10. 1 hit.
InterProi IPR027640. Kinesin-like_fam.
IPR019821. Kinesin_motor_CS.
IPR001752. Kinesin_motor_dom.
IPR027417. P-loop_NTPase.
[Graphical view ]
PANTHERi PTHR24115. PTHR24115. 1 hit.
Pfami PF00225. Kinesin. 1 hit.
[Graphical view ]
PRINTSi PR00380. KINESINHEAVY.
SMARTi SM00129. KISc. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
PROSITEi PS00411. KINESIN_MOTOR_1. 1 hit.
PS50067. KINESIN_MOTOR_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Suppression of the expression of a pancreatic beta-cell form of the kinesin heavy chain by antisense oligonucleotides inhibits insulin secretion from primary cultures of mouse beta-cells."
    Meng Y.X., Wilson G.W., Avery M.C., Varden C.H., Balczon R.
    Endocrinology 138:1979-1987(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Pancreas.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain.
  3. "Cloning mammalian genes by expression selection of genetic suppressor elements: association of kinesin with drug resistance and cell immortalization."
    Gudkov A.V., Kazarov A.R., Thimmapaya R., Axenovich S.A., Mazo I.A., Roninson I.B.
    Proc. Natl. Acad. Sci. U.S.A. 91:3744-3748(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-881.
    Strain: BALB/c.
  4. Lubec G., Sunyer B., Chen W.-Q.
    Submitted (JAN-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 132-141 AND 490-505, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: OF1.
    Tissue: Hippocampus.
  5. "Targeted disruption of mouse conventional kinesin heavy chain, kif5B, results in abnormal perinuclear clustering of mitochondria."
    Tanaka Y., Kanai Y., Okada Y., Nonaka S., Takeda S., Harada A., Hirokawa N.
    Cell 93:1147-1158(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  6. "Glutamate-receptor-interacting protein GRIP1 directly steers kinesin to dendrites."
    Setou M., Seog D.-H., Tanaka Y., Kanai Y., Takei Y., Kawagishi M., Hirokawa N.
    Nature 417:83-87(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GRIP1.
  7. "Marlin-1 and conventional kinesin link GABAB receptors to the cytoskeleton and regulate receptor transport."
    Vidal R.L., Ramirez O.A., Sandoval L., Koenig-Robert R., Haertel S., Couve A.
    Mol. Cell. Neurosci. 35:501-512(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH JAKMIP1.
  8. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-945, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  9. "TLRR (lrrc67) interacts with PP1 and is associated with a cytoskeletal complex in the testis."
    Wang R., Kaul A., Sperry A.O.
    Biol. Cell 102:173-189(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PPP1R42.

Entry informationi

Entry nameiKINH_MOUSE
AccessioniPrimary (citable) accession number: Q61768
Secondary accession number(s): O08711, Q5BL10, Q61580
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 27, 2011
Last modified: September 3, 2014
This is version 119 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi