Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Potassium voltage-gated channel subfamily A member 5

Gene

Kcna5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Voltage-gated potassium channel that mediates transmembrane potassium transport in excitable membranes. Forms tetrameric potassium-selective channels through which potassium ions pass in accordance with their electrochemical gradient. The channel alternates between opened and closed conformations in response to the voltage difference across the membrane (PubMed:8226976, PubMed:11349004). Can form functional homotetrameric channels and heterotetrameric channels that contain variable proportions of KCNA1, KCNA2, KCNA4, KCNA5, and possibly other family members as well; channel properties depend on the type of alpha subunits that are part of the channel (By similarity). Channel properties are modulated by cytoplasmic beta subunits that regulate the subcellular location of the alpha subunits and promote rapid inactivation (By similarity). Homotetrameric channels display rapid activation and slow inactivation (PubMed:8226976, PubMed:11349004). May play a role in regulating the secretion of insulin in normal pancreatic islets (By similarity).By similarity2 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Potassium channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

Potassium

Enzyme and pathway databases

ReactomeiR-MMU-1296072. Voltage gated Potassium channels.

Names & Taxonomyi

Protein namesi
Recommended name:
Potassium voltage-gated channel subfamily A member 5
Alternative name(s):
Voltage-gated potassium channel subunit Kv1.5
Short name:
KV1-5
Gene namesi
Name:Kcna5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:96662. Kcna5.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 238CytoplasmicBy similarityAdd BLAST238
Transmembranei239 – 260Helical; Name=Segment S1By similarityAdd BLAST22
Topological domaini261 – 314ExtracellularBy similarityAdd BLAST54
Transmembranei315 – 336Helical; Name=Segment S2By similarityAdd BLAST22
Topological domaini337 – 347CytoplasmicBy similarityAdd BLAST11
Transmembranei348 – 368Helical; Name=Segment S3By similarityAdd BLAST21
Topological domaini369 – 384ExtracellularBy similarityAdd BLAST16
Transmembranei385 – 405Helical; Voltage-sensor; Name=Segment S4By similarityAdd BLAST21
Topological domaini406 – 420CytoplasmicBy similarityAdd BLAST15
Transmembranei421 – 442Helical; Name=Segment S5By similarityAdd BLAST22
Topological domaini443 – 456ExtracellularBy similarityAdd BLAST14
Intramembranei457 – 468Helical; Name=Pore helixBy similarityAdd BLAST12
Intramembranei469 – 476By similarity8
Topological domaini477 – 483ExtracellularBy similarity7
Transmembranei484 – 512Helical; Name=Segment S6By similarityAdd BLAST29
Topological domaini513 – 602CytoplasmicBy similarityAdd BLAST90

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

No visible phenotype. The action potential in myocytes is not prolonged by low concentrations of 4-aminopyridine, contrary to the situation in wild-type.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000539861 – 602Potassium voltage-gated channel subfamily A member 5Add BLAST602

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei81Phosphoserine; by CK2 and PKASequence analysis1
Cross-linki212Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Glycosylationi290N-linked (GlcNAc...)Sequence analysis1
Lipidationi337S-palmitoyl cysteineSequence analysis1
Cross-linki525Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Modified residuei535Phosphoserine; by PKASequence analysis1
Modified residuei546Phosphoserine; by PKASequence analysis1
Modified residuei569Phosphoserine; by PKASequence analysis1

Post-translational modificationi

Sumoylated on Lys-212, and Lys-525, preferentially with SUMO3. Sumoylation regulates the voltage sensitivity of the channel (By similarity).By similarity

Keywords - PTMi

Glycoprotein, Isopeptide bond, Lipoprotein, Palmitate, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ61762.
PeptideAtlasiQ61762.
PRIDEiQ61762.

PTM databases

iPTMnetiQ61762.
PhosphoSitePlusiQ61762.

Expressioni

Tissue specificityi

Highly expressed in heart and moderately in brain. Low levels in thymus, skeletal muscle and spleen. Not expressed in liver, lung or kidney.

Gene expression databases

BgeeiENSMUSG00000045534.
GenevisibleiQ61762. MM.

Interactioni

Subunit structurei

Homotetramer and heterotetramer of potassium channel proteins. Interacts with DLG1, which enhances channel currents. Forms a ternary complex with DLG1 and CAV3 (By similarity). Interacts with KCNAB1 (By similarity). Interacts with UBE2I (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi200880. 1 interactor.
STRINGi10090.ENSMUSP00000055673.

Structurei

3D structure databases

ProteinModelPortaliQ61762.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni407 – 420S4-S5 linkerBy similarityAdd BLAST14

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi469 – 474Selectivity filterBy similarity6
Motifi600 – 602PDZ-binding3

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi373 – 376Poly-Gly4

Domaini

The amino terminus may be important in determining the rate of inactivation of the channel while the C-terminal PDZ-binding motif may play a role in modulation of channel activity and/or targeting of the channel to specific subcellular compartments.By similarity
The transmembrane segment S4 functions as voltage-sensor and is characterized by a series of positively charged amino acids at every third position. Channel opening and closing is effected by a conformation change that affects the position and orientation of the voltage-sensor paddle formed by S3 and S4 within the membrane. A transmembrane electric field that is positive inside would push the positively charged S4 segment outwards, thereby opening the pore, while a field that is negative inside would pull the S4 segment inwards and close the pore. Changes in the position and orientation of S4 are then transmitted to the activation gate formed by the inner helix bundle via the S4-S5 linker region.By similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1545. Eukaryota.
COG1226. LUCA.
GeneTreeiENSGT00760000118846.
HOGENOMiHOG000231015.
HOVERGENiHBG052230.
InParanoidiQ61762.
KOiK04878.
OMAiGGELQCP.
OrthoDBiEOG091G10NU.
TreeFamiTF313103.

Family and domain databases

Gene3Di1.20.120.350. 1 hit.
InterProiIPR000210. BTB/POZ_dom.
IPR027359. Channel_four-helix_dom.
IPR005821. Ion_trans_dom.
IPR003968. K_chnl_volt-dep_Kv.
IPR003972. K_chnl_volt-dep_Kv1.
IPR004052. K_chnl_volt-dep_Kv1.5.
IPR011333. SKP1/BTB/POZ.
IPR003131. T1-type_BTB.
IPR028325. VG_K_chnl.
[Graphical view]
PANTHERiPTHR11537. PTHR11537. 2 hits.
PTHR11537:SF25. PTHR11537:SF25. 2 hits.
PfamiPF02214. BTB_2. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view]
PRINTSiPR00169. KCHANNEL.
PR01512. KV15CHANNEL.
PR01491. KVCHANNEL.
PR01496. SHAKERCHANEL.
SMARTiSM00225. BTB. 1 hit.
[Graphical view]
SUPFAMiSSF54695. SSF54695. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q61762-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEISLVPMEN GSAMTLRGGG EAGASCVQSP RGECGCPPTA GLNNQSKETS
60 70 80 90 100
PRRRATHEDA GQGGRPLPPM PQELPQPRRP SAEDEEGEGD PGLGTVEEDQ
110 120 130 140 150
APQDSGSLHH QRVLINISGL RFETQLGTLA QFPNTLLGDP VKRLRYFDPL
160 170 180 190 200
RNEYFFDRNR PSFDGILYYY QSGGRLRRPV NVSLDVFADE IRFYQLGDEA
210 220 230 240 250
MERFREDEGF IKEEEKPLPR NEFQRQVWLI FEYPESSGSA RAIAIVSVLV
260 270 280 290 300
ILISIITFCL ETLPEFRDER ELLRHPPVPP QPPAPAPGAN GSGSGVLSSG
310 320 330 340 350
PTVAPLLPRT LADPFFIVET TCVIWFTFEL LVRFFACPSK AEFSRNIMNI
360 370 380 390 400
IDIVAIFPYF ITLGTELAEQ QPGGGGQNGQ QAMSLAILRV IRLVRVFRIF
410 420 430 440 450
KLSRHSKGLQ ILGKTLQASM RELGLLIFFL FIGVILFSSA VYFAEADNQG
460 470 480 490 500
SHFSSIPDAF WWAVVTMTTV GYGDMRPITV GGKIVGSLCA IAGVLTIALP
510 520 530 540 550
VPVIVSNFNY FYHRETDHEE QAALKEEQGI QRRESGLDTG GQRKVSCSKA
560 570 580 590 600
SFCKTGGPLE STDSIRRGSC PLEKCHLKAK SNVDLRRSLY ALCLDTSRET

DL
Length:602
Mass (Da):66,580
Last modified:July 27, 2011 - v2
Checksum:i02926E85DC022DDA
GO
Isoform 2 (identifier: Q61762-2) [UniParc]FASTAAdd to basket
Also known as: 5'

The sequence of this isoform differs from the canonical sequence as follows:
     1-200: Missing.

Show »
Length:402
Mass (Da):44,539
Checksum:i6BB846FB2409473D
GO
Isoform 3 (identifier: Q61762-3) [UniParc]FASTAAdd to basket
Also known as: 3'

The sequence of this isoform differs from the canonical sequence as follows:
     515-602: Missing.

Note: Inactive. Inhibits expression of isoform 1 and isoform 2.
Show »
Length:514
Mass (Da):56,871
Checksum:i9A047A2DE9D6DC7B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti8M → T in AAA39365 (PubMed:8226976).Curated1
Sequence conflicti23G → V in AAA39365 (PubMed:8226976).Curated1
Sequence conflicti61G → A in AAA39365 (PubMed:8226976).Curated1
Sequence conflicti127G → D in AAA39365 (PubMed:8226976).Curated1
Sequence conflicti130A → V in AAA39365 (PubMed:8226976).Curated1
Sequence conflicti135T → N in AAA39365 (PubMed:8226976).Curated1
Sequence conflicti249L → S in AAA39365 (PubMed:8226976).Curated1
Sequence conflicti268 – 269DE → VD in AAA39365 (PubMed:8226976).Curated2
Sequence conflicti274R → L in AAA39365 (PubMed:8226976).Curated1
Sequence conflicti289 – 291ANG → TNA in AAA39365 (PubMed:8226976).Curated3
Sequence conflicti301P → T in AAA39365 (PubMed:8226976).Curated1
Sequence conflicti452 – 453HF → QL in AAA39365 (PubMed:8226976).Curated2
Sequence conflicti553C → H in AAA39365 (PubMed:8226976).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0009611 – 200Missing in isoform 2. 1 PublicationAdd BLAST200
Alternative sequenceiVSP_000962515 – 602Missing in isoform 3. 1 PublicationAdd BLAST88

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L22218 mRNA. Translation: AAA39365.1.
AF108659 mRNA. Translation: AAD13779.1.
AF302768 Genomic DNA. Translation: AAG40241.1.
AK146843 mRNA. Translation: BAE27474.1.
CH466523 Genomic DNA. Translation: EDK99840.1.
BC021787 mRNA. Translation: AAH21787.1.
CCDSiCCDS20554.1. [Q61762-1]
PIRiA49507.
RefSeqiNP_666095.1. NM_145983.2. [Q61762-1]
UniGeneiMm.222831.

Genome annotation databases

EnsembliENSMUST00000060972; ENSMUSP00000055673; ENSMUSG00000045534. [Q61762-1]
GeneIDi16493.
KEGGimmu:16493.
UCSCiuc009dva.2. mouse. [Q61762-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L22218 mRNA. Translation: AAA39365.1.
AF108659 mRNA. Translation: AAD13779.1.
AF302768 Genomic DNA. Translation: AAG40241.1.
AK146843 mRNA. Translation: BAE27474.1.
CH466523 Genomic DNA. Translation: EDK99840.1.
BC021787 mRNA. Translation: AAH21787.1.
CCDSiCCDS20554.1. [Q61762-1]
PIRiA49507.
RefSeqiNP_666095.1. NM_145983.2. [Q61762-1]
UniGeneiMm.222831.

3D structure databases

ProteinModelPortaliQ61762.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200880. 1 interactor.
STRINGi10090.ENSMUSP00000055673.

PTM databases

iPTMnetiQ61762.
PhosphoSitePlusiQ61762.

Proteomic databases

PaxDbiQ61762.
PeptideAtlasiQ61762.
PRIDEiQ61762.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000060972; ENSMUSP00000055673; ENSMUSG00000045534. [Q61762-1]
GeneIDi16493.
KEGGimmu:16493.
UCSCiuc009dva.2. mouse. [Q61762-1]

Organism-specific databases

CTDi3741.
MGIiMGI:96662. Kcna5.

Phylogenomic databases

eggNOGiKOG1545. Eukaryota.
COG1226. LUCA.
GeneTreeiENSGT00760000118846.
HOGENOMiHOG000231015.
HOVERGENiHBG052230.
InParanoidiQ61762.
KOiK04878.
OMAiGGELQCP.
OrthoDBiEOG091G10NU.
TreeFamiTF313103.

Enzyme and pathway databases

ReactomeiR-MMU-1296072. Voltage gated Potassium channels.

Miscellaneous databases

PROiQ61762.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000045534.
GenevisibleiQ61762. MM.

Family and domain databases

Gene3Di1.20.120.350. 1 hit.
InterProiIPR000210. BTB/POZ_dom.
IPR027359. Channel_four-helix_dom.
IPR005821. Ion_trans_dom.
IPR003968. K_chnl_volt-dep_Kv.
IPR003972. K_chnl_volt-dep_Kv1.
IPR004052. K_chnl_volt-dep_Kv1.5.
IPR011333. SKP1/BTB/POZ.
IPR003131. T1-type_BTB.
IPR028325. VG_K_chnl.
[Graphical view]
PANTHERiPTHR11537. PTHR11537. 2 hits.
PTHR11537:SF25. PTHR11537:SF25. 2 hits.
PfamiPF02214. BTB_2. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view]
PRINTSiPR00169. KCHANNEL.
PR01512. KV15CHANNEL.
PR01491. KVCHANNEL.
PR01496. SHAKERCHANEL.
SMARTiSM00225. BTB. 1 hit.
[Graphical view]
SUPFAMiSSF54695. SSF54695. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiKCNA5_MOUSE
AccessioniPrimary (citable) accession number: Q61762
Secondary accession number(s): Q9Z1R6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 150 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.