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Q61759

- K1B21_MOUSE

UniProt

Q61759 - K1B21_MOUSE

Protein

Kallikrein 1-related peptidase b21

Gene

Klk1b21

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 3 (07 Jun 2004)
      Previous versions | rss
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    Functioni

    Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin. Displays trypsin-like substrate specificity and shows activity towards casein, gelatin, fibronectin and IGFBP3.1 Publication

    Catalytic activityi

    Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.Curated

    Enzyme regulationi

    Inhibited by protease inhibitors diisopropylfluorophosphate, leupeptin, antipain, benzamidine, phenylmethylsulfonyl fluoride and soybean trypsin inhibitor.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei65 – 651Charge relay systemBy similarity
    Active sitei120 – 1201Charge relay systemBy similarity
    Active sitei213 – 2131Charge relay systemBy similarity

    GO - Molecular functioni

    1. peptidase activity Source: MGI
    2. serine-type endopeptidase activity Source: InterPro

    GO - Biological processi

    1. proteolysis Source: MGI

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Enzyme and pathway databases

    ReactomeiREACT_199000. Activation of Matrix Metalloproteinases.

    Protein family/group databases

    MEROPSiS01.038.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Kallikrein 1-related peptidase b21 (EC:3.4.21.35)
    Alternative name(s):
    Glandular kallikrein K21
    Short name:
    mGK-21
    Tissue kallikrein 21
    Gene namesi
    Name:Klk1b21
    Synonyms:Klk-21, Klk21
    OrganismiMus musculus (Mouse)Imported
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 7

    Organism-specific databases

    MGIiMGI:892022. Klk1b21.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular space Source: MGI

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1717Sequence AnalysisAdd
    BLAST
    Propeptidei18 – 247Activation peptideBy similarityPRO_0000027987
    Chaini25 – 261237Kallikrein 1-related peptidase b21PRO_0000027988Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi31 ↔ 173By similarityPROSITE-ProRule annotation
    Disulfide bondi50 ↔ 66By similarityPROSITE-ProRule annotation
    Glycosylationi102 – 1021N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi152 ↔ 219By similarityPROSITE-ProRule annotation
    Disulfide bondi184 ↔ 198By similarityPROSITE-ProRule annotation
    Disulfide bondi209 ↔ 234By similarityPROSITE-ProRule annotation

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Zymogen

    Proteomic databases

    PaxDbiQ61759.
    PRIDEiQ61759.

    Expressioni

    Tissue specificityi

    Expressed in testis and submaxillary gland. In the testis, expression localized specifically to Leydig cells in the interstitial tissues.1 Publication

    Developmental stagei

    Detectable in testis 4 weeks after birth, becoming more prominent thereafter.1 Publication

    Inductioni

    By T protein.1 Publication

    Gene expression databases

    ArrayExpressiQ61759.
    BgeeiQ61759.
    CleanExiMM_KLK1B21.
    GenevestigatoriQ61759.

    Structurei

    3D structure databases

    ProteinModelPortaliQ61759.
    SMRiQ61759. Positions 25-261.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini25 – 258234Peptidase S1PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase S1 family. Kallikrein subfamily.PROSITE-ProRule annotation
    Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG5640.
    GeneTreeiENSGT00750000117405.
    HOGENOMiHOG000251820.
    HOVERGENiHBG013304.
    InParanoidiQ61759.
    KOiK01325.
    OMAiHESSAQH.
    OrthoDBiEOG75B84T.
    PhylomeDBiQ61759.
    TreeFamiTF331065.

    Family and domain databases

    InterProiIPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view]
    PfamiPF00089. Trypsin. 1 hit.
    [Graphical view]
    PRINTSiPR00722. CHYMOTRYPSIN.
    SMARTiSM00020. Tryp_SPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50494. SSF50494. 1 hit.
    PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q61759-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRFLILFLAL SLGEIDAAPP VQSRIVGGFN CEKNSQPWHV AVFRYNKYIC    50
    GGVLLNPNWV LTAAHCYGNQ YNVWLGKNKL FQHESSAQHR LVSKSFPHPD 100
    YNMSLMNDHT PHPEDDYSND LMLLRLSKPA DITDAVKPID LPTEEPKLGS 150
    TCLASGWGSI TPTKWQIPND LQCGFIKPLP NENCAKAYIH KVTDVMLCAG 200
    EMGGGKDTCA GDSGGPLICD GVLQGITSWG SIPCAKPNAP AIYTKLIKFT 250
    SWIKDTMAKN P 261
    Length:261
    Mass (Da):28,690
    Last modified:June 7, 2004 - v3
    Checksum:i608B976BC78E03EE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB039276 mRNA. Translation: BAA92319.1.
    BC012243 mRNA. Translation: AAH12243.1.
    M18597 Genomic DNA. Translation: AAA39359.1.
    M18617 Genomic DNA. Translation: AAA39360.1.
    CCDSiCCDS21195.1.
    PIRiI70036.
    I70037.
    RefSeqiNP_034772.1. NM_010642.2.
    UniGeneiMm.443292.

    Genome annotation databases

    EnsembliENSMUST00000085455; ENSMUSP00000082582; ENSMUSG00000066516.
    GeneIDi16616.
    KEGGimmu:16616.
    UCSCiuc009goh.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB039276 mRNA. Translation: BAA92319.1 .
    BC012243 mRNA. Translation: AAH12243.1 .
    M18597 Genomic DNA. Translation: AAA39359.1 .
    M18617 Genomic DNA. Translation: AAA39360.1 .
    CCDSi CCDS21195.1.
    PIRi I70036.
    I70037.
    RefSeqi NP_034772.1. NM_010642.2.
    UniGenei Mm.443292.

    3D structure databases

    ProteinModelPortali Q61759.
    SMRi Q61759. Positions 25-261.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi S01.038.

    Proteomic databases

    PaxDbi Q61759.
    PRIDEi Q61759.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000085455 ; ENSMUSP00000082582 ; ENSMUSG00000066516 .
    GeneIDi 16616.
    KEGGi mmu:16616.
    UCSCi uc009goh.1. mouse.

    Organism-specific databases

    CTDi 16616.
    MGIi MGI:892022. Klk1b21.

    Phylogenomic databases

    eggNOGi COG5640.
    GeneTreei ENSGT00750000117405.
    HOGENOMi HOG000251820.
    HOVERGENi HBG013304.
    InParanoidi Q61759.
    KOi K01325.
    OMAi HESSAQH.
    OrthoDBi EOG75B84T.
    PhylomeDBi Q61759.
    TreeFami TF331065.

    Enzyme and pathway databases

    Reactomei REACT_199000. Activation of Matrix Metalloproteinases.

    Miscellaneous databases

    NextBioi 290211.
    PROi Q61759.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q61759.
    Bgeei Q61759.
    CleanExi MM_KLK1B21.
    Genevestigatori Q61759.

    Family and domain databases

    InterProi IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view ]
    Pfami PF00089. Trypsin. 1 hit.
    [Graphical view ]
    PRINTSi PR00722. CHYMOTRYPSIN.
    SMARTi SM00020. Tryp_SPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50494. SSF50494. 1 hit.
    PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of mouse Klk27, a novel tissue kallikrein expressed in testicular Leydig cells and exhibiting chymotrypsin-like specificity."
      Matsui H., Moriyama A., Takahashi T.
      Eur. J. Biochem. 267:6858-6865(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
      Tissue: Testis1 Publication.
    2. "Mouse testicular Leydig cells express Klk21, a tissue kallikrein that cleaves fibronectin and IGF-binding protein-3."
      Matsui H., Takahashi T.
      Endocrinology 142:4918-4929(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE, FUNCTION, ENZYME REGULATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION.
      Tissue: Testis1 Publication.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/NImported.
      Tissue: Salivary glandImported.
    4. "Mouse glandular kallikrein genes. Structure and partial sequence analysis of the kallikrein gene locus."
      Evans B.A., Drinkwater C.C., Richards R.I.
      J. Biol. Chem. 262:8027-8034(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE OF 17-54 AND 70-122.
      Strain: BALB/c1 Publication.
      Tissue: Liver1 Publication.

    Entry informationi

    Entry nameiK1B21_MOUSE
    AccessioniPrimary (citable) accession number: Q61759
    Secondary accession number(s): Q61760, Q9JM70
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 7, 2004
    Last sequence update: June 7, 2004
    Last modified: October 1, 2014
    This is version 104 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3