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Protein

Kallikrein 1-related peptidase b21

Gene

Klk1b21

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin. Displays trypsin-like substrate specificity and shows activity towards casein, gelatin, fibronectin and IGFBP3.1 Publication

Catalytic activityi

Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.Curated

Enzyme regulationi

Inhibited by protease inhibitors diisopropylfluorophosphate, leupeptin, antipain, benzamidine, phenylmethylsulfonyl fluoride and soybean trypsin inhibitor.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei65Charge relay systemBy similarity1
Active sitei120Charge relay systemBy similarity1
Active sitei213Charge relay systemBy similarity1

GO - Molecular functioni

GO - Biological processi

  • proteolysis Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Enzyme and pathway databases

ReactomeiR-MMU-1592389. Activation of Matrix Metalloproteinases.
R-MMU-381426. Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs).

Protein family/group databases

MEROPSiS01.038.

Names & Taxonomyi

Protein namesi
Recommended name:
Kallikrein 1-related peptidase b21 (EC:3.4.21.35)
Alternative name(s):
Glandular kallikrein K21
Short name:
mGK-21
Tissue kallikrein 21
Gene namesi
Name:Klk1b21
Synonyms:Klk-21, Klk21
OrganismiMus musculus (Mouse)Imported
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:892022. Klk1b21.

Subcellular locationi

GO - Cellular componenti

  • extracellular exosome Source: MGI
  • extracellular space Source: MGI
  • nucleus Source: MGI
  • protein complex Source: MGI
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 17Sequence analysisAdd BLAST17
PropeptideiPRO_000002798718 – 24Activation peptideBy similarity7
ChainiPRO_000002798825 – 261Kallikrein 1-related peptidase b21Add BLAST237

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi31 ↔ 173PROSITE-ProRule annotationBy similarity
Disulfide bondi50 ↔ 66PROSITE-ProRule annotationBy similarity
Glycosylationi102N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi152 ↔ 219PROSITE-ProRule annotationBy similarity
Disulfide bondi184 ↔ 198PROSITE-ProRule annotationBy similarity
Disulfide bondi209 ↔ 234PROSITE-ProRule annotationBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiQ61759.
PRIDEiQ61759.

Expressioni

Tissue specificityi

Expressed in testis and submaxillary gland. In the testis, expression localized specifically to Leydig cells in the interstitial tissues.1 Publication

Developmental stagei

Detectable in testis 4 weeks after birth, becoming more prominent thereafter.1 Publication

Inductioni

By T protein.1 Publication

Gene expression databases

BgeeiENSMUSG00000066516.
CleanExiMM_KLK1B21.
ExpressionAtlasiQ61759. baseline and differential.
GenevisibleiQ61759. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000082582.

Structurei

3D structure databases

ProteinModelPortaliQ61759.
SMRiQ61759.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini25 – 258Peptidase S1PROSITE-ProRule annotationAdd BLAST234

Sequence similaritiesi

Belongs to the peptidase S1 family. Kallikrein subfamily.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00760000118862.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiQ61759.
KOiK01325.
OMAiHESSAQH.
OrthoDBiEOG091G0DF7.
PhylomeDBiQ61759.
TreeFamiTF331065.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q61759-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRFLILFLAL SLGEIDAAPP VQSRIVGGFN CEKNSQPWHV AVFRYNKYIC
60 70 80 90 100
GGVLLNPNWV LTAAHCYGNQ YNVWLGKNKL FQHESSAQHR LVSKSFPHPD
110 120 130 140 150
YNMSLMNDHT PHPEDDYSND LMLLRLSKPA DITDAVKPID LPTEEPKLGS
160 170 180 190 200
TCLASGWGSI TPTKWQIPND LQCGFIKPLP NENCAKAYIH KVTDVMLCAG
210 220 230 240 250
EMGGGKDTCA GDSGGPLICD GVLQGITSWG SIPCAKPNAP AIYTKLIKFT
260
SWIKDTMAKN P
Length:261
Mass (Da):28,690
Last modified:June 7, 2004 - v3
Checksum:i608B976BC78E03EE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB039276 mRNA. Translation: BAA92319.1.
BC012243 mRNA. Translation: AAH12243.1.
M18597 Genomic DNA. Translation: AAA39359.1.
M18617 Genomic DNA. Translation: AAA39360.1.
CCDSiCCDS21195.1.
PIRiI70036.
I70037.
RefSeqiNP_034772.1. NM_010642.3.
UniGeneiMm.443292.

Genome annotation databases

EnsembliENSMUST00000085455; ENSMUSP00000082582; ENSMUSG00000066516.
GeneIDi16616.
KEGGimmu:16616.
UCSCiuc009goh.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB039276 mRNA. Translation: BAA92319.1.
BC012243 mRNA. Translation: AAH12243.1.
M18597 Genomic DNA. Translation: AAA39359.1.
M18617 Genomic DNA. Translation: AAA39360.1.
CCDSiCCDS21195.1.
PIRiI70036.
I70037.
RefSeqiNP_034772.1. NM_010642.3.
UniGeneiMm.443292.

3D structure databases

ProteinModelPortaliQ61759.
SMRiQ61759.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000082582.

Protein family/group databases

MEROPSiS01.038.

Proteomic databases

PaxDbiQ61759.
PRIDEiQ61759.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000085455; ENSMUSP00000082582; ENSMUSG00000066516.
GeneIDi16616.
KEGGimmu:16616.
UCSCiuc009goh.1. mouse.

Organism-specific databases

CTDi16616.
MGIiMGI:892022. Klk1b21.

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00760000118862.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiQ61759.
KOiK01325.
OMAiHESSAQH.
OrthoDBiEOG091G0DF7.
PhylomeDBiQ61759.
TreeFamiTF331065.

Enzyme and pathway databases

ReactomeiR-MMU-1592389. Activation of Matrix Metalloproteinases.
R-MMU-381426. Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs).

Miscellaneous databases

PROiQ61759.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000066516.
CleanExiMM_KLK1B21.
ExpressionAtlasiQ61759. baseline and differential.
GenevisibleiQ61759. MM.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiK1B21_MOUSE
AccessioniPrimary (citable) accession number: Q61759
Secondary accession number(s): Q61760, Q9JM70
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: June 7, 2004
Last modified: November 2, 2016
This is version 123 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.