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Q61759

- K1B21_MOUSE

UniProt

Q61759 - K1B21_MOUSE

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Protein
Kallikrein 1-related peptidase b21
Gene
Klk1b21, Klk-21, Klk21
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin. Displays trypsin-like substrate specificity and shows activity towards casein, gelatin, fibronectin and IGFBP3.1 Publication

Catalytic activityi

Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.

Enzyme regulationi

Inhibited by protease inhibitors diisopropylfluorophosphate, leupeptin, antipain, benzamidine, phenylmethylsulfonyl fluoride and soybean trypsin inhibitor.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei65 – 651Charge relay system By similarityBy similarity
Active sitei120 – 1201Charge relay system By similarityBy similarity
Active sitei213 – 2131Charge relay system By similarityBy similarity

GO - Molecular functioni

  1. peptidase activity Source: MGI
  2. serine-type endopeptidase activity Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. proteolysis Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Enzyme and pathway databases

ReactomeiREACT_199000. Activation of Matrix Metalloproteinases.

Protein family/group databases

MEROPSiS01.038.

Names & Taxonomyi

Protein namesi
Recommended name:
Kallikrein 1-related peptidase b21 (EC:3.4.21.35)
Alternative name(s):
Glandular kallikrein K21
Short name:
mGK-21
Tissue kallikrein 21
Gene namesi
Name:Klk1b21
Synonyms:Klk-21, Klk21
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 7

Organism-specific databases

MGIiMGI:892022. Klk1b21.

Subcellular locationi

GO - Cellular componenti

  1. extracellular space Source: MGI
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1717 Reviewed prediction
Add
BLAST
Propeptidei18 – 247Activation peptide By similarityBy similarity
PRO_0000027987
Chaini25 – 261237Kallikrein 1-related peptidase b21
PRO_0000027988Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi31 ↔ 173 By similarityBy similarity
Disulfide bondi50 ↔ 66 By similarityBy similarity
Glycosylationi102 – 1021N-linked (GlcNAc...) Reviewed predictionBy similarity
Disulfide bondi152 ↔ 219 By similarityBy similarity
Disulfide bondi184 ↔ 198 By similarityBy similarity
Disulfide bondi209 ↔ 234 By similarityBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiQ61759.
PRIDEiQ61759.

Expressioni

Tissue specificityi

Expressed in testis and submaxillary gland. In the testis, expression localized specifically to Leydig cells in the interstitial tissues.1 Publication

Developmental stagei

Detectable in testis 4 weeks after birth, becoming more prominent thereafter.1 Publication

Inductioni

By T protein.1 Publication

Gene expression databases

ArrayExpressiQ61759.
BgeeiQ61759.
CleanExiMM_KLK1B21.
GenevestigatoriQ61759.

Structurei

3D structure databases

ProteinModelPortaliQ61759.
SMRiQ61759. Positions 25-261.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini25 – 258234Peptidase S1
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG5640.
GeneTreeiENSGT00750000117405.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiQ61759.
KOiK01325.
OMAiHESSAQH.
OrthoDBiEOG75B84T.
PhylomeDBiQ61759.
TreeFamiTF331065.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q61759-1 [UniParc]FASTAAdd to Basket

« Hide

MRFLILFLAL SLGEIDAAPP VQSRIVGGFN CEKNSQPWHV AVFRYNKYIC    50
GGVLLNPNWV LTAAHCYGNQ YNVWLGKNKL FQHESSAQHR LVSKSFPHPD 100
YNMSLMNDHT PHPEDDYSND LMLLRLSKPA DITDAVKPID LPTEEPKLGS 150
TCLASGWGSI TPTKWQIPND LQCGFIKPLP NENCAKAYIH KVTDVMLCAG 200
EMGGGKDTCA GDSGGPLICD GVLQGITSWG SIPCAKPNAP AIYTKLIKFT 250
SWIKDTMAKN P 261
Length:261
Mass (Da):28,690
Last modified:June 7, 2004 - v3
Checksum:i608B976BC78E03EE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB039276 mRNA. Translation: BAA92319.1.
BC012243 mRNA. Translation: AAH12243.1.
M18597 Genomic DNA. Translation: AAA39359.1.
M18617 Genomic DNA. Translation: AAA39360.1.
CCDSiCCDS21195.1.
PIRiI70036.
I70037.
RefSeqiNP_034772.1. NM_010642.2.
UniGeneiMm.443292.

Genome annotation databases

EnsembliENSMUST00000085455; ENSMUSP00000082582; ENSMUSG00000066516.
GeneIDi16616.
KEGGimmu:16616.
UCSCiuc009goh.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB039276 mRNA. Translation: BAA92319.1 .
BC012243 mRNA. Translation: AAH12243.1 .
M18597 Genomic DNA. Translation: AAA39359.1 .
M18617 Genomic DNA. Translation: AAA39360.1 .
CCDSi CCDS21195.1.
PIRi I70036.
I70037.
RefSeqi NP_034772.1. NM_010642.2.
UniGenei Mm.443292.

3D structure databases

ProteinModelPortali Q61759.
SMRi Q61759. Positions 25-261.
ModBasei Search...

Protein family/group databases

MEROPSi S01.038.

Proteomic databases

PaxDbi Q61759.
PRIDEi Q61759.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000085455 ; ENSMUSP00000082582 ; ENSMUSG00000066516 .
GeneIDi 16616.
KEGGi mmu:16616.
UCSCi uc009goh.1. mouse.

Organism-specific databases

CTDi 16616.
MGIi MGI:892022. Klk1b21.

Phylogenomic databases

eggNOGi COG5640.
GeneTreei ENSGT00750000117405.
HOGENOMi HOG000251820.
HOVERGENi HBG013304.
InParanoidi Q61759.
KOi K01325.
OMAi HESSAQH.
OrthoDBi EOG75B84T.
PhylomeDBi Q61759.
TreeFami TF331065.

Enzyme and pathway databases

Reactomei REACT_199000. Activation of Matrix Metalloproteinases.

Miscellaneous databases

NextBioi 290211.
PROi Q61759.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q61759.
Bgeei Q61759.
CleanExi MM_KLK1B21.
Genevestigatori Q61759.

Family and domain databases

InterProi IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view ]
Pfami PF00089. Trypsin. 1 hit.
[Graphical view ]
PRINTSi PR00722. CHYMOTRYPSIN.
SMARTi SM00020. Tryp_SPc. 1 hit.
[Graphical view ]
SUPFAMi SSF50494. SSF50494. 1 hit.
PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of mouse Klk27, a novel tissue kallikrein expressed in testicular Leydig cells and exhibiting chymotrypsin-like specificity."
    Matsui H., Moriyama A., Takahashi T.
    Eur. J. Biochem. 267:6858-6865(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Tissue: Testis.
  2. "Mouse testicular Leydig cells express Klk21, a tissue kallikrein that cleaves fibronectin and IGF-binding protein-3."
    Matsui H., Takahashi T.
    Endocrinology 142:4918-4929(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE, FUNCTION, ENZYME REGULATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION.
    Tissue: Testis.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Salivary gland.
  4. "Mouse glandular kallikrein genes. Structure and partial sequence analysis of the kallikrein gene locus."
    Evans B.A., Drinkwater C.C., Richards R.I.
    J. Biol. Chem. 262:8027-8034(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE OF 17-54 AND 70-122.
    Strain: BALB/c.
    Tissue: Liver.

Entry informationi

Entry nameiK1B21_MOUSE
AccessioniPrimary (citable) accession number: Q61759
Secondary accession number(s): Q61760, Q9JM70
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: June 7, 2004
Last modified: September 3, 2014
This is version 103 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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