Reviewed, UniProtKB/Swiss-Prot Q61759 (K1B21_MOUSE)

Names and origin

Protein names

Recommended name:
    Kallikrein 1-related peptidase b21
    EC=3.4.21.35
Alternative name(s):
    Tissue kallikrein 21
    Glandular kallikrein K21
      Short name=mGK-21

Gene names

Name:	Klk1b21
Synonyms:	Klk-21, Klk21

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	261 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin. Displays trypsin-like substrate specificity and shows activity towards casein, gelatin, fibronectin and IGFBP3. Ref.2
Catalytic activity	Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.
Enzyme regulation	Inhibited by protease inhibitors diisopropylfluorophosphate, leupeptin, antipain, benzamidine, phenylmethylsulfonyl fluoride and soybean trypsin inhibitor. Ref.2
Tissue specificity	Expressed in testis and submaxillary gland. In the testis, expression localized specifically to Leydig cells in the interstitial tissues. Ref.2
Developmental stage	Detectable in testis 4 weeks after birth, becoming more prominent thereafter. Ref.2
Induction	By T protein. Ref.2
Sequence similarities	Belongs to the peptidase S1 family. Kallikrein subfamily. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Glycoprotein Zymogen
Gene Ontology (GO)
Biological process	proteolysis Ref.2 Inferred from direct assay. Source: MGI
Cellular component	extracellular space Ref.2 Inferred from direct assay. Source: MGI
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	Potential
	Propeptide	18 – 24	7	Activation peptide By similarity UniProtKB P36368		PRO_0000027987
	Chain	25 – 261	237	Kallikrein 1-related peptidase b21		PRO_0000027988
Regions
	Domain	25 – 258	234	Peptidase S1
Sites
	Active site	65	1	Charge relay system By similarity UniProtKB P36368
	Active site	120	1	Charge relay system By similarity UniProtKB P36368
	Active site	213	1	Charge relay system By similarity UniProtKB P36368
Amino acid modifications
	Glycosylation	102	1	N-linked (GlcNAc...) Potential UniProtKB P36368
	Disulfide bond	31 ↔ 173		By similarity UniProtKB P36368
	Disulfide bond	50 ↔ 66		By similarity UniProtKB P36368
	Disulfide bond	152 ↔ 219		By similarity UniProtKB P36368
	Disulfide bond	184 ↔ 198		By similarity UniProtKB P36368
	Disulfide bond	209 ↔ 234		By similarity UniProtKB P36368

Sequences

	Sequence		Length	Mass (Da)
	Q61759-1 [UniParc]. Last modified June 7, 2004. Version 3. Checksum: 608B976BC78E03EE	FASTA	261	28,690
	10 20 30 40 50 60 MRFLILFLAL SLGEIDAAPP VQSRIVGGFN CEKNSQPWHV AVFRYNKYIC GGVLLNPNWV 70 80 90 100 110 120 LTAAHCYGNQ YNVWLGKNKL FQHESSAQHR LVSKSFPHPD YNMSLMNDHT PHPEDDYSND 130 140 150 160 170 180 LMLLRLSKPA DITDAVKPID LPTEEPKLGS TCLASGWGSI TPTKWQIPND LQCGFIKPLP 190 200 210 220 230 240 NENCAKAYIH KVTDVMLCAG EMGGGKDTCA GDSGGPLICD GVLQGITSWG SIPCAKPNAP 250 260 AIYTKLIKFT SWIKDTMAKN P

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of mouse Klk27, a novel tissue kallikrein expressed in testicular Leydig cells and exhibiting chymotrypsin-like specificity." Matsui H., Moriyama A., Takahashi T. Eur. J. Biochem. 267:6858-6865(2000) [PubMed: 11082197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Tissue: Testis.
[2]	"Mouse testicular Leydig cells express Klk21, a tissue kallikrein that cleaves fibronectin and IGF-binding protein-3." Matsui H., Takahashi T. Endocrinology 142:4918-4929(2001) [PubMed: 11606460] [Abstract] Cited for: NUCLEOTIDE SEQUENCE, FUNCTION, ENZYME REGULATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION. Tissue: Testis.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Salivary gland.
[4]	"Mouse glandular kallikrein genes. Structure and partial sequence analysis of the kallikrein gene locus." Evans B.A., Drinkwater C.C., Richards R.I. J. Biol. Chem. 262:8027-8034(1987) [PubMed: 3036794] [Abstract] Cited for: NUCLEOTIDE SEQUENCE OF 17-54 AND 70-122. Strain: BALB/c. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB039276 mRNA. Translation: BAA92319.1. BC012243 mRNA. Translation: AAH12243.1. M18597 Genomic DNA. Translation: AAA39359.1. M18617 Genomic DNA. Translation: AAA39360.1.
IPI	IPI00124822.
PIR	I70036. I70037.
RefSeq	NP_034772.1.
UniGene	Mm.443292
3D structure databases
HSSP	HSSP built from PDB template 1SGF based on UniProtKB P00757.
SMR	Q61759. Positions 25-261.
ModBase	Search...
Protein family/group databases
MEROPS	S01.038.
Genome annotation databases
Ensembl	ENSMUSG00000066516. Mus musculus. [Contig view]
GeneID	16616.
KEGG	mmu:16616.
Organism-specific databases
MGI	MGI:892022. Klk1b21.
Phylogenomic databases
HOVERGEN	Q61759.
OMA	Q61759. FQHESSA.
Enzyme and pathway databases
BRENDA	3.4.21.35. 244.
Gene expression databases
ArrayExpress	Q61759.
Bgee	Q61759.
CleanEx	MM_KLK1B21.
GermOnline	ENSMUSG00000066516. Mus musculus.
Family and domain databases
InterPro	IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	290211.
SOURCE	Search...

Entry information

Entry name

K1B21_MOUSE

Accession

Primary (citable) accession number: Q61759
Secondary accession number(s): Q61760, Q9JM70

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2004
Last sequence update:	June 7, 2004
Last modified:	May 5, 2009
This is version 62 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)