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Q61759 (K1B21_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Kallikrein 1-related peptidase b21

EC=3.4.21.35
Alternative name(s):
Glandular kallikrein K21
Short name=mGK-21
Tissue kallikrein 21
Gene names
Name:Klk1b21
Synonyms:Klk-21, Klk21
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length261 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin. Displays trypsin-like substrate specificity and shows activity towards casein, gelatin, fibronectin and IGFBP3. Ref.2

Catalytic activity

Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.

Enzyme regulation

Inhibited by protease inhibitors diisopropylfluorophosphate, leupeptin, antipain, benzamidine, phenylmethylsulfonyl fluoride and soybean trypsin inhibitor. Ref.2

Tissue specificity

Expressed in testis and submaxillary gland. In the testis, expression localized specifically to Leydig cells in the interstitial tissues. Ref.2

Developmental stage

Detectable in testis 4 weeks after birth, becoming more prominent thereafter. Ref.2

Induction

By T protein. Ref.2

Sequence similarities

Belongs to the peptidase S1 family. Kallikrein subfamily.

Contains 1 peptidase S1 domain.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1717 Potential
Propeptide18 – 247Activation peptide By similarity UniProtKB P36368
PRO_0000027987
Chain25 – 261237Kallikrein 1-related peptidase b21
PRO_0000027988

Regions

Domain25 – 258234Peptidase S1

Sites

Active site651Charge relay system By similarity UniProtKB P36368
Active site1201Charge relay system By similarity UniProtKB P36368
Active site2131Charge relay system By similarity UniProtKB P36368

Amino acid modifications

Glycosylation1021N-linked (GlcNAc...) Potential UniProtKB P36368
Disulfide bond31 ↔ 173 By similarity UniProtKB P36368
Disulfide bond50 ↔ 66 By similarity UniProtKB P36368
Disulfide bond152 ↔ 219 By similarity UniProtKB P36368
Disulfide bond184 ↔ 198 By similarity UniProtKB P36368
Disulfide bond209 ↔ 234 By similarity UniProtKB P36368

Sequences

Sequence LengthMass (Da)Tools
Q61759 [UniParc].

Last modified June 7, 2004. Version 3.
Checksum: 608B976BC78E03EE

FASTA26128,690
        10         20         30         40         50         60 
MRFLILFLAL SLGEIDAAPP VQSRIVGGFN CEKNSQPWHV AVFRYNKYIC GGVLLNPNWV 

        70         80         90        100        110        120 
LTAAHCYGNQ YNVWLGKNKL FQHESSAQHR LVSKSFPHPD YNMSLMNDHT PHPEDDYSND 

       130        140        150        160        170        180 
LMLLRLSKPA DITDAVKPID LPTEEPKLGS TCLASGWGSI TPTKWQIPND LQCGFIKPLP 

       190        200        210        220        230        240 
NENCAKAYIH KVTDVMLCAG EMGGGKDTCA GDSGGPLICD GVLQGITSWG SIPCAKPNAP 

       250        260 
AIYTKLIKFT SWIKDTMAKN P 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and characterization of mouse Klk27, a novel tissue kallikrein expressed in testicular Leydig cells and exhibiting chymotrypsin-like specificity."
Matsui H., Moriyama A., Takahashi T.
Eur. J. Biochem. 267:6858-6865(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Tissue: Testis.
[2]"Mouse testicular Leydig cells express Klk21, a tissue kallikrein that cleaves fibronectin and IGF-binding protein-3."
Matsui H., Takahashi T.
Endocrinology 142:4918-4929(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE, FUNCTION, ENZYME REGULATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION.
Tissue: Testis.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Salivary gland.
[4]"Mouse glandular kallikrein genes. Structure and partial sequence analysis of the kallikrein gene locus."
Evans B.A., Drinkwater C.C., Richards R.I.
J. Biol. Chem. 262:8027-8034(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE OF 17-54 AND 70-122.
Strain: BALB/c.
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB039276 mRNA. Translation: BAA92319.1.
BC012243 mRNA. Translation: AAH12243.1.
M18597 Genomic DNA. Translation: AAA39359.1.
M18617 Genomic DNA. Translation: AAA39360.1.
CCDSCCDS21195.1.
PIRI70036.
I70037.
RefSeqNP_034772.1. NM_010642.2.
UniGeneMm.443292.

3D structure databases

ProteinModelPortalQ61759.
SMRQ61759. Positions 25-261.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSS01.038.

Proteomic databases

PaxDbQ61759.
PRIDEQ61759.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000085455; ENSMUSP00000082582; ENSMUSG00000066516.
GeneID16616.
KEGGmmu:16616.
UCSCuc009goh.1. mouse.

Organism-specific databases

CTD16616.
MGIMGI:892022. Klk1b21.

Phylogenomic databases

eggNOGCOG5640.
GeneTreeENSGT00750000117405.
HOGENOMHOG000251820.
HOVERGENHBG013304.
InParanoidQ61759.
KOK01325.
OMAHESSAQH.
OrthoDBEOG75B84T.
PhylomeDBQ61759.
TreeFamTF331065.

Gene expression databases

ArrayExpressQ61759.
BgeeQ61759.
CleanExMM_KLK1B21.
GenevestigatorQ61759.

Family and domain databases

InterProIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSPR00722. CHYMOTRYPSIN.
SMARTSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMSSF50494. SSF50494. 1 hit.
PROSITEPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio290211.
PROQ61759.
SOURCESearch...

Entry information

Entry nameK1B21_MOUSE
AccessionPrimary (citable) accession number: Q61759
Secondary accession number(s): Q61760, Q9JM70
Entry history
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: June 7, 2004
Last modified: July 9, 2014
This is version 102 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot