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Q61754 (K1B24_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Kallikrein 1-related peptidase b24

EC=3.4.21.35
Alternative name(s):
Glandular kallikrein K24
Short name=mGK-24
Tissue kallikrein 24
Gene names
Name:Klk1b24
Synonyms:Klk-24, Klk24
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length263 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.

Catalytic activity

Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.

Sequence similarities

Belongs to the peptidase S1 family. Kallikrein subfamily.

Contains 1 peptidase S1 domain.

Ontologies

Keywords
   DomainSignal
   Molecular functionHydrolase
Protease
Serine protease
   PTMDisulfide bond
Glycoprotein
Zymogen
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processproteolysis

Non-traceable author statement Ref.1. Source: UniProtKB

   Molecular_functionserine-type endopeptidase activity

Non-traceable author statement Ref.1. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1717 Potential
Propeptide18 – 247Activation peptide By similarity UniProtKB P36368
PRO_0000027991
Chain25 – 263239Kallikrein 1-related peptidase b24
PRO_0000027992

Regions

Domain25 – 260236Peptidase S1

Sites

Active site651Charge relay system By similarity UniProtKB P36368
Active site1221Charge relay system By similarity UniProtKB P36368
Active site2151Charge relay system By similarity UniProtKB P36368

Amino acid modifications

Glycosylation691N-linked (GlcNAc...) Potential
Glycosylation1051N-linked (GlcNAc...) Potential UniProtKB P36368
Glycosylation1851N-linked (GlcNAc...) Potential
Disulfide bond31 ↔ 175 By similarity UniProtKB P36368
Disulfide bond50 ↔ 66 By similarity UniProtKB P36368
Disulfide bond154 ↔ 221 By similarity UniProtKB P36368
Disulfide bond186 ↔ 200 By similarity UniProtKB P36368
Disulfide bond211 ↔ 236 By similarity UniProtKB P36368

Sequences

Sequence LengthMass (Da)Tools
Q61754 [UniParc].

Last modified June 7, 2004. Version 3.
Checksum: 4EE052242B7A415F

FASTA26328,926
        10         20         30         40         50         60 
MWFLILFLAL SLGGIDAAPP VQSRVVGGFK CEKNSQPWHV AVFRYNKYIC GGVLLNPNWV 

        70         80         90        100        110        120 
LTAAHCYGNA TSQYNVWLGK NKLFQREPSA QHRWVSKSFP HPDYNMSLLN DDIPQPKDKS 

       130        140        150        160        170        180 
NDLMLLRLSE PADITDAVKP IDLPTEEPKL GSTCLASGWG SITPTKWQKP NDLQCVFIKL 

       190        200        210        220        230        240 
LPNENCTKPY LHKVTDVMLC AGEMGGGKDT CAGDSGGPLI CDGILHGITS WGPVPCGKPN 

       250        260 
APAIYTKLIK FASWIKDTMA KNP 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and characterization of mouse Klk27, a novel tissue kallikrein expressed in testicular Leydig cells and exhibiting chymotrypsin-like specificity."
Matsui H., Moriyama A., Takahashi T.
Eur. J. Biochem. 267:6858-6865(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Testis.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[3]"Mouse glandular kallikrein genes. Structure and partial sequence analysis of the kallikrein gene locus."
Evans B.A., Drinkwater C.C., Richards R.I.
J. Biol. Chem. 262:8027-8034(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 17-54 AND 73-124.
Strain: BALB/c.
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB039277 mRNA. Translation: BAA92320.1.
BC146015 mRNA. Translation: AAI46016.1.
BC146019 mRNA. Translation: AAI46020.1.
M18619, M18599 Genomic DNA. Translation: AAG11390.1.
CCDSCCDS21198.1.
PIRI70016.
I70017.
RefSeqNP_034773.1. NM_010643.1.
UniGeneMm.483098.

3D structure databases

ProteinModelPortalQ61754.
SMRQ61754. Positions 25-262.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000073392.

Protein family/group databases

MEROPSS01.069.

PTM databases

PhosphoSiteQ61754.

Proteomic databases

MaxQBQ61754.
PaxDbQ61754.
PRIDEQ61754.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000073713; ENSMUSP00000073392; ENSMUSG00000063713.
GeneID16617.
KEGGmmu:16617.
UCSCuc009gok.1. mouse.

Organism-specific databases

CTD16617.
MGIMGI:892021. Klk1b24.

Phylogenomic databases

eggNOGCOG5640.
GeneTreeENSGT00750000117405.
HOGENOMHOG000251820.
HOVERGENHBG013304.
InParanoidA6H6V7.
KOK01325.
OMALTWINDT.
OrthoDBEOG7VDXPV.
PhylomeDBQ61754.
TreeFamTF331065.

Gene expression databases

ArrayExpressQ61754.
BgeeQ61754.
CleanExMM_KLK1B24.
GenevestigatorQ61754.

Family and domain databases

InterProIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSPR00722. CHYMOTRYPSIN.
SMARTSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMSSF50494. SSF50494. 1 hit.
PROSITEPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio290215.
PROQ61754.
SOURCESearch...

Entry information

Entry nameK1B24_MOUSE
AccessionPrimary (citable) accession number: Q61754
Secondary accession number(s): A6H6V7, Q61755, Q9JM69
Entry history
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: June 7, 2004
Last modified: July 9, 2014
This is version 98 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot