Reviewed, UniProtKB/Swiss-Prot Q61754 (K1B24_MOUSE)

Names and origin

Protein names

Recommended name:
    Kallikrein 1-related peptidase b24
    EC=3.4.21.35
Alternative name(s):
    Glandular kallikrein K24
      Short name=mGK-24
    Tissue kallikrein 24

Gene names

Name:	Klk1b24
Synonyms:	Klk-24, Klk24

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	263 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.
Catalytic activity	Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.
Sequence similarities	Belongs to the peptidase S1 family. Kallikrein subfamily. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Glycoprotein Zymogen
Gene Ontology (GO)
Biological process	proteolysis Ref.1 Non-traceable author statement. Source: UniProtKB
Molecular function	serine-type endopeptidase activity Ref.1 Non-traceable author statement. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	Potential
	Propeptide	18 – 24	7	Activation peptide By similarity UniProtKB P36368		PRO_0000027991
	Chain	25 – 263	239	Kallikrein 1-related peptidase b24		PRO_0000027992
Regions
	Domain	25 – 260	236	Peptidase S1
Sites
	Active site	65	1	Charge relay system By similarity UniProtKB P36368
	Active site	122	1	Charge relay system By similarity UniProtKB P36368
	Active site	215	1	Charge relay system By similarity UniProtKB P36368
Amino acid modifications
	Glycosylation	69	1	N-linked (GlcNAc...) Potential
	Glycosylation	105	1	N-linked (GlcNAc...) Potential UniProtKB P36368
	Glycosylation	185	1	N-linked (GlcNAc...) Potential
	Disulfide bond	31 ↔ 175		By similarity UniProtKB P36368
	Disulfide bond	50 ↔ 66		By similarity UniProtKB P36368
	Disulfide bond	154 ↔ 221		By similarity UniProtKB P36368
	Disulfide bond	186 ↔ 200		By similarity UniProtKB P36368
	Disulfide bond	211 ↔ 236		By similarity UniProtKB P36368

Sequences

	Sequence		Length	Mass (Da)
	Q61754-1 [UniParc]. Last modified June 7, 2004. Version 3. Checksum: 4EE052242B7A415F	FASTA	263	28,926
	10 20 30 40 50 60 MWFLILFLAL SLGGIDAAPP VQSRVVGGFK CEKNSQPWHV AVFRYNKYIC GGVLLNPNWV 70 80 90 100 110 120 LTAAHCYGNA TSQYNVWLGK NKLFQREPSA QHRWVSKSFP HPDYNMSLLN DDIPQPKDKS 130 140 150 160 170 180 NDLMLLRLSE PADITDAVKP IDLPTEEPKL GSTCLASGWG SITPTKWQKP NDLQCVFIKL 190 200 210 220 230 240 LPNENCTKPY LHKVTDVMLC AGEMGGGKDT CAGDSGGPLI CDGILHGITS WGPVPCGKPN 250 260 APAIYTKLIK FASWIKDTMA KNP

References

[1]	"Cloning and characterization of mouse Klk27, a novel tissue kallikrein expressed in testicular Leydig cells and exhibiting chymotrypsin-like specificity." Matsui H., Moriyama A., Takahashi T. Eur. J. Biochem. 267:6858-6865(2000) [PubMed: 11082197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Tissue: Testis.
[2]	"Mouse glandular kallikrein genes. Structure and partial sequence analysis of the kallikrein gene locus." Evans B.A., Drinkwater C.C., Richards R.I. J. Biol. Chem. 262:8027-8034(1987) [PubMed: 3036794] [Abstract] Cited for: NUCLEOTIDE SEQUENCE OF 17-54 AND 73-124. Strain: BALB/c. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB039277 mRNA. Translation: BAA92320.1. M18619, M18599 Genomic DNA. Translation: AAG11390.1.
IPI	IPI00124804.
PIR	I70016. I70017.
RefSeq	NP_034773.1.
UniGene	Mm.444308
3D structure databases
HSSP	HSSP built from PDB template 1SGF based on UniProtKB P00757.
SMR	Q61754. Positions 25-262.
ModBase	Search...
Genome annotation databases
Ensembl	ENSMUSG00000063713. Mus musculus. [Contig view]
GeneID	16617.
KEGG	mmu:16617.
Organism-specific databases
MGI	MGI:892021. Klk1b24.
Phylogenomic databases
HOVERGEN	Q61754.
OMA	Q61754. CVNINIL.
Enzyme and pathway databases
BRENDA	3.4.21.35. 244.
Gene expression databases
ArrayExpress	Q61754.
Bgee	Q61754.
CleanEx	MM_KLK1B24.
GermOnline	ENSMUSG00000063713. Mus musculus.
Family and domain databases
InterPro	IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	290215.
SOURCE	Search...

Entry information

Entry name

K1B24_MOUSE

Accession

Primary (citable) accession number: Q61754
Secondary accession number(s): Q61755, Q9JM69

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2004
Last sequence update:	June 7, 2004
Last modified:	May 26, 2009
This is version 57 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)