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Q61743 (IRK11_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 118. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
ATP-sensitive inward rectifier potassium channel 11
Alternative name(s):
Inward rectifier K(+) channel Kir6.2
Potassium channel, inwardly rectifying subfamily J member 11
Gene names
Name:Kcnj11
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length390 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This receptor is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium. Can form cardiac and smooth muscle-type KATP channels with ABCC9. KCNJ11 forms the channel pore while ABCC9 is required for activation and regulation By similarity.

Subunit structure

Interacts with ABCC9/SUR2 By similarity. Interacts with ABCC8/SUR.

Subcellular location

Membrane; Multi-pass membrane protein.

Post-translational modification

Phosphorylation by MAPK1 results in changes in channel gating that destabilize the closed states and reduce the ATP sensitivity By similarity.

Sequence similarities

Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ11 subfamily. [View classification]

Ontologies

Keywords
   Biological processIon transport
Potassium transport
Transport
   Cellular componentMembrane
   DomainTransmembrane
Transmembrane helix
   LigandPotassium
   Molecular functionIon channel
Voltage-gated channel
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcellular response to glucose stimulus

Inferred from electronic annotation. Source: Ensembl

cellular response to nicotine

Inferred from electronic annotation. Source: Ensembl

cellular response to tumor necrosis factor

Inferred from electronic annotation. Source: Ensembl

glucose metabolic process

Inferred from electronic annotation. Source: Ensembl

negative regulation of insulin secretion

Inferred from electronic annotation. Source: Ensembl

neurological system process

Inferred from electronic annotation. Source: Ensembl

positive regulation of cation channel activity

Inferred from electronic annotation. Source: Ensembl

potassium ion import

Inferred from direct assay PubMed 8630239. Source: BHF-UCL

regulation of membrane potential

Inferred from electronic annotation. Source: Ensembl

response to ATP

Inferred from electronic annotation. Source: Ensembl

response to drug

Inferred from electronic annotation. Source: Ensembl

response to estradiol

Inferred from electronic annotation. Source: Ensembl

response to ischemia

Inferred from electronic annotation. Source: Ensembl

response to testosterone

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentATP-sensitive potassium channel complex

Inferred from direct assay PubMed 8630239. Source: BHF-UCL

T-tubule

Inferred from direct assay PubMed 20610380. Source: BHF-UCL

axolemma

Inferred from electronic annotation. Source: Ensembl

cell body fiber

Inferred from electronic annotation. Source: Ensembl

cytosol

Inferred from electronic annotation. Source: Ensembl

endoplasmic reticulum

Inferred from electronic annotation. Source: Ensembl

endosome

Inferred from electronic annotation. Source: Ensembl

intercalated disc

Inferred from electronic annotation. Source: Ensembl

mitochondrion

Inferred from electronic annotation. Source: Ensembl

myelin sheath

Inferred from electronic annotation. Source: Ensembl

neuronal cell body

Inferred from electronic annotation. Source: Ensembl

nuclear envelope

Inferred from electronic annotation. Source: Ensembl

plasma membrane

Inferred from direct assay PubMed 16085792. Source: MGI

voltage-gated potassium channel complex

Inferred from electronic annotation. Source: Ensembl

   Molecular_functionATP binding

Inferred from direct assay PubMed 12860923. Source: BHF-UCL

ATP-activated inward rectifier potassium channel activity

Inferred from direct assay PubMed 8630239. Source: BHF-UCL

ankyrin binding

Inferred from physical interaction PubMed 20610380. Source: BHF-UCL

potassium ion binding

Inferred by curator PubMed 8630239. Source: BHF-UCL

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

AP2A2O949732EBI-8603527,EBI-1642835From a different organism.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 390390ATP-sensitive inward rectifier potassium channel 11
PRO_0000154958

Regions

Topological domain1 – 6868Cytoplasmic By similarity
Transmembrane69 – 9325Helical; Name=M1; By similarity
Topological domain94 – 11623Extracellular By similarity
Intramembrane117 – 12812Helical; Pore-forming; Name=H5; By similarity
Intramembrane129 – 1357Pore-forming; By similarity
Topological domain136 – 1449Extracellular By similarity
Transmembrane145 – 16622Helical; Name=M2; By similarity
Topological domain167 – 390224Cytoplasmic By similarity
Motif130 – 1356Selectivity filter By similarity

Sites

Site1601Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesium By similarity

Amino acid modifications

Modified residue3411Phosphothreonine; by MAPK1 By similarity
Modified residue3851Phosphoserine; by MAPK1 By similarity

Experimental info

Sequence conflict2481G → S in AAD02096. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q61743 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 6AFBFFD284C92C3A

FASTA39043,562
        10         20         30         40         50         60 
MLSRKGIIPE EYVLTRLAED PAEPRYRTRE RRARFVSKKG NCNVAHKNIR EQGRFLQDVF 

        70         80         90        100        110        120 
TTLVDLKWPH TLLIFTMSFL CSWLLFAMVW WLIAFAHGDL APGEGTNVPC VTSIHSFSSA 

       130        140        150        160        170        180 
FLFSIEVQVT IGFGGRMVTE ECPLAILILI VQNIVGLMIN AIMLGCIFMK TAQAHRRAET 

       190        200        210        220        230        240 
LIFSKHAVIT LRHGRLCFML RVGDLRKSMI ISATIHMQVV RKTTSPEGEV VPLHQVDIPM 

       250        260        270        280        290        300 
ENGVGGNGIF LVAPLIIYHV IDSNSPLYDL APSDLHHHQD LEIIVILEGV VETTGITTQA 

       310        320        330        340        350        360 
RTSYLADEIL WGQRFVPIVA EEDGRYSVDY SKFGNTIKVP TPLCTARQLD EDRSLLDALT 

       370        380        390 
LASSRGPLRK RSVAVAKAKP KFSISPDSLS 

« Hide

References

« Hide 'large scale' references
[1]"Reconstitution of IKATP: an inward rectifier subunit plus the sulfonylurea receptor."
Inagaki N., Gonoi T., Clement J.P. IV, Namba N., Inazawa J., Gonzalez G., Aguilar-Bryan L., Seino S., Bryan J.
Science 270:1166-1170(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C57BL/6.
Tissue: Pancreatic islet.
[2]"Cloning and functional expression of the cDNA encoding a novel ATP-sensitive potassium channel subunit expressed in pancreatic beta-cells, brain, heart and skeletal muscle."
Sakura H., Aemmaelae C., Smith P.A., Gribble F.M., Ashcroft F.M.
FEBS Lett. 377:338-344(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Tissue: Pancreatic islet.
[3]"Structural characterization of the mouse sulfonylurea receptor-1 and potassium inward rectifier 6.2 genes."
Kooptiwut S., Shelton K.D., Magnuson M.A.
Submitted (DEC-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 129/Ola.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D50581 mRNA. Translation: BAA09130.1.
U73626 mRNA. Translation: AAB17355.1.
AF037313 Genomic DNA. Translation: AAD02096.1.
BC052731 mRNA. Translation: AAH52731.1.
BC057006 mRNA. Translation: AAH57006.1.
PIRS68403.
RefSeqNP_034732.1. NM_010602.3.
UniGeneMm.333863.
Mm.428799.

3D structure databases

ProteinModelPortalQ61743.
SMRQ61743. Positions 32-356.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid200896. 1 interaction.
DIPDIP-42790N.
IntActQ61743. 1 interaction.
MINTMINT-2982880.

Chemistry

GuidetoPHARMACOLOGY442.

PTM databases

PhosphoSiteQ61743.

Proteomic databases

PRIDEQ61743.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000180081; ENSMUSP00000136002; ENSMUSG00000096146.
GeneID16514.
KEGGmmu:16514.
UCSCuc009gyc.2. mouse.

Organism-specific databases

CTD3767.
MGIMGI:107501. Kcnj11.

Phylogenomic databases

eggNOGNOG72812.
GeneTreeENSGT00690000102074.
HOGENOMHOG000237325.
HOVERGENHBG006178.
InParanoidQ61743.
KOK05004.
OMAHHQDLEI.
OrthoDBEOG7XPZ5K.
PhylomeDBQ61743.
TreeFamTF313676.

Gene expression databases

BgeeQ61743.
GenevestigatorQ61743.

Family and domain databases

Gene3D2.60.40.1400. 1 hit.
InterProIPR014756. Ig_E-set.
IPR016449. K_chnl_inward-rec_Kir.
IPR003279. K_chnl_inward-rec_Kir6.2.
IPR013518. K_chnl_inward-rec_Kir_cyto.
[Graphical view]
PANTHERPTHR11767. PTHR11767. 1 hit.
PfamPF01007. IRK. 1 hit.
[Graphical view]
PIRSFPIRSF005465. GIRK_kir. 1 hit.
PRINTSPR01332. KIR62CHANNEL.
PR01320. KIRCHANNEL.
SUPFAMSSF81296. SSF81296. 1 hit.
ProtoNetSearch...

Other

NextBio289865.
PROQ61743.
SOURCESearch...

Entry information

Entry nameIRK11_MOUSE
AccessionPrimary (citable) accession number: Q61743
Secondary accession number(s): Q9QX21
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot