Q61743 (IRK11_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP-sensitive inward rectifier potassium channel 11

Alternative name(s):
Inward rectifier K(+) channel Kir6.2
Potassium channel, inwardly rectifying subfamily J member 11

Gene names

Name:

Kcnj11

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	390 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	This receptor is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium. Can form cardiac and smooth muscle-type KATP channels with ABCC9. KCNJ11 forms the channel pore while ABCC9 is required for activation and regulation By similarity.
Subunit structure	Interacts with ABCC9/SUR2 By similarity. Interacts with ABCC8/SUR.
Subcellular location	Membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ11 subfamily. [View classification]

Ontologies

Keywords
Biological process	Ion transport Potassium transport Transport
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Potassium
Molecular function	Ion channel Voltage-gated channel
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glucose metabolic process Inferred from electronic annotation. Source: Compara negative regulation of insulin secretion Inferred from electronic annotation. Source: Compara neurological system process Inferred from electronic annotation. Source: Compara potassium ion import Inferred from direct assay PubMed 8630239. Source: BHF-UCL regulation of membrane potential Inferred from electronic annotation. Source: Compara response to ATP Inferred from electronic annotation. Source: Compara response to drug Inferred from electronic annotation. Source: Compara response to testosterone stimulus Inferred from electronic annotation. Source: Compara
Cellular_component	ATP-sensitive potassium channel complex Inferred from direct assay PubMed 8630239. Source: BHF-UCL T-tubule Inferred from direct assay PubMed 20610380. Source: BHF-UCL endoplasmic reticulum Inferred from electronic annotation. Source: Compara mitochondrion Inferred from electronic annotation. Source: Compara voltage-gated potassium channel complex Inferred from electronic annotation. Source: Compara
Molecular_function	ATP binding Inferred from direct assay PubMed 12860923. Source: BHF-UCL ATP-activated inward rectifier potassium channel activity Inferred from direct assay PubMed 8630239. Source: BHF-UCL potassium ion binding Inferred by curator PubMed 8630239. Source: BHF-UCL
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 390	390	ATP-sensitive inward rectifier potassium channel 11		PRO_0000154958
Regions
	Topological domain	1 – 68	68	Cytoplasmic By similarity
	Transmembrane	69 – 93	25	Helical; Name=M1; By similarity
	Topological domain	94 – 116	23	Extracellular By similarity
	Intramembrane	117 – 128	12	Helical; Pore-forming; Name=H5; By similarity
	Intramembrane	129 – 135	7	Pore-forming; By similarity
	Topological domain	136 – 144	9	Extracellular By similarity
	Transmembrane	145 – 166	22	Helical; Name=M2; By similarity
	Topological domain	167 – 390	224	Cytoplasmic By similarity
	Motif	130 – 135	6	Selectivity filter By similarity
Sites
	Site	160	1	Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesium By similarity
Experimental info
	Sequence conflict	248	1	G → S in AAD02096. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q61743 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 6AFBFFD284C92C3A	FASTA	390	43,562
	10 20 30 40 50 60 MLSRKGIIPE EYVLTRLAED PAEPRYRTRE RRARFVSKKG NCNVAHKNIR EQGRFLQDVF 70 80 90 100 110 120 TTLVDLKWPH TLLIFTMSFL CSWLLFAMVW WLIAFAHGDL APGEGTNVPC VTSIHSFSSA 130 140 150 160 170 180 FLFSIEVQVT IGFGGRMVTE ECPLAILILI VQNIVGLMIN AIMLGCIFMK TAQAHRRAET 190 200 210 220 230 240 LIFSKHAVIT LRHGRLCFML RVGDLRKSMI ISATIHMQVV RKTTSPEGEV VPLHQVDIPM 250 260 270 280 290 300 ENGVGGNGIF LVAPLIIYHV IDSNSPLYDL APSDLHHHQD LEIIVILEGV VETTGITTQA 310 320 330 340 350 360 RTSYLADEIL WGQRFVPIVA EEDGRYSVDY SKFGNTIKVP TPLCTARQLD EDRSLLDALT 370 380 390 LASSRGPLRK RSVAVAKAKP KFSISPDSLS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Reconstitution of IKATP: an inward rectifier subunit plus the sulfonylurea receptor." Inagaki N., Gonoi T., Clement J.P. IV, Namba N., Inazawa J., Gonzalez G., Aguilar-Bryan L., Seino S., Bryan J. Science 270:1166-1170(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6. Tissue: Pancreatic islet.
[2]	"Cloning and functional expression of the cDNA encoding a novel ATP-sensitive potassium channel subunit expressed in pancreatic beta-cells, brain, heart and skeletal muscle." Sakura H., Aemmaelae C., Smith P.A., Gribble F.M., Ashcroft F.M. FEBS Lett. 377:338-344(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Tissue: Pancreatic islet.
[3]	"Structural characterization of the mouse sulfonylurea receptor-1 and potassium inward rectifier 6.2 genes." Kooptiwut S., Shelton K.D., Magnuson M.A. Submitted (DEC-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129/Ola.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D50581 mRNA. Translation: BAA09130.1. U73626 mRNA. Translation: AAB17355.1. AF037313 Genomic DNA. Translation: AAD02096.1. BC052731 mRNA. Translation: AAH52731.1. BC057006 mRNA. Translation: AAH57006.1.
IPI	IPI00124866.
PIR	S68403.
RefSeq	NP_034732.1. NM_010602.3.
UniGene	Mm.333863. Mm.428799.
3D structure databases
ProteinModelPortal	Q61743.
SMR	Q61743. Positions 32-356.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-42790N.
MINT	MINT-2982880.
PTM databases
PhosphoSite	Q61743.
Proteomic databases
PRIDE	Q61743.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000180081; ENSMUSP00000136002; ENSMUSG00000096146.
GeneID	16514.
KEGG	mmu:16514.
UCSC	uc009gyc.1. mouse.
Organism-specific databases
CTD	3767.
MGI	MGI:107501. Kcnj11.
Phylogenomic databases
eggNOG	NOG72812.
GeneTree	ENSGT00690000101708.
HOGENOM	HOG000237325.
HOVERGEN	HBG006178.
InParanoid	Q61743.
KO	K05004.
OMA	EEYVLTR.
OrthoDB	EOG4DFPNM.
Gene expression databases
ArrayExpress	Q61743.
Bgee	Q61743.
Genevestigator	Q61743.
GermOnline	ENSMUSG00000070561. Mus musculus.
Family and domain databases
Gene3D	2.60.40.1400. 1 hit.
InterPro	IPR014756. Ig_E-set. IPR016449. K_chnl_inward-rec_Kir. IPR003279. K_chnl_inward-rec_Kir6.2. IPR013518. K_chnl_inward-rec_Kir_cyto. [Graphical view]
PANTHER	PTHR11767. PTHR11767. 1 hit.
Pfam	PF01007. IRK. 1 hit. [Graphical view]
PIRSF	PIRSF005465. GIRK_kir. 1 hit.
PRINTS	PR01332. KIR62CHANNEL. PR01320. KIRCHANNEL.
SUPFAM	SSF81296. Ig_E-set. 1 hit.
ProtoNet	Search...
Other
ChEMBL	CHEMBL3330.
NextBio	289865.
SOURCE	Search...

Entry information

Entry name

IRK11_MOUSE

Accession

Primary (citable) accession number: Q61743
Secondary accession number(s): Q9QX21

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 110 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program