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Q61739 (ITA6_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 130. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Integrin alpha-6
Alternative name(s):
CD49 antigen-like family member F
VLA-6
CD_antigen=CD49f
Gene names
Name:Itga6
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1091 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Integrin alpha-6/beta-1 is a receptor for laminin on platelets. Integrin alpha-6/beta-4 is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome. Mice expressing a null mutation of the alpha-6 subunit gene die soon after birth and develop severe blistering. The blisters are due to separation of the basal epithelial cells from a normally formed basement membrane.

Subunit structure

Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of a heavy and a light chain linked by a disulfide bond. Alpha-6 associates with either beta-1 or beta-4. Interacts with RAB21 By similarity.

Subcellular location

Cell membrane; Single-pass type I membrane protein. Cell membrane; Lipid-anchor By similarity.

Post-translational modification

Isoforms containing segment A, but not segment B, are the major targets for PMA-induced phosphorylation. Phosphorylation occurs on 'Ser-1064' of isoform alpha-6X1A Phosphorylation is not required for the induction of integrin alpha-6A/beta-1 high affinity but may reduce the affinity for ligand By similarity.

Palmitoylation by DHHC3 enhances stability and cell surface expression By similarity.

Sequence similarities

Belongs to the integrin alpha chain family.

Contains 7 FG-GAP repeats.

Ontologies

Keywords
   Biological processCell adhesion
   Cellular componentCell membrane
Membrane
   Coding sequence diversityAlternative splicing
   DomainRepeat
Signal
Transmembrane
Transmembrane helix
   LigandCalcium
Metal-binding
   Molecular functionIntegrin
Receptor
   PTMCleavage on pair of basic residues
Disulfide bond
Glycoprotein
Lipoprotein
Palmitate
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processbrown fat cell differentiation

Inferred from direct assay PubMed 18492766. Source: MGI

cell adhesion mediated by integrin

Inferred from direct assay PubMed 1833411. Source: MGI

cell-matrix adhesion

Inferred from mutant phenotype PubMed 16365040. Source: MGI

cellular response to extracellular stimulus

Inferred from direct assay PubMed 10322635. Source: MGI

cellular response to organic cyclic compound

Inferred from electronic annotation. Source: Ensembl

filopodium assembly

Inferred from mutant phenotype PubMed 16365040. Source: MGI

integrin-mediated signaling pathway

Inferred from electronic annotation. Source: UniProtKB-KW

leukocyte migration

Inferred from mutant phenotype PubMed 7621877. Source: MGI

negative regulation of extrinsic apoptotic signaling pathway

Inferred from electronic annotation. Source: Ensembl

odontogenesis of dentin-containing tooth

Inferred from mutant phenotype PubMed 16365040. Source: MGI

positive regulation of apoptotic process

Inferred from electronic annotation. Source: Ensembl

positive regulation of cell-cell adhesion

Inferred from direct assay PubMed 7669058. Source: MGI

positive regulation of cell-substrate adhesion

Inferred from electronic annotation. Source: Ensembl

positive regulation of phosphorylation

Inferred from electronic annotation. Source: Ensembl

positive regulation of transcription from RNA polymerase II promoter

Inferred from electronic annotation. Source: Ensembl

single organismal cell-cell adhesion

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentalpha6-beta4 integrin complex

Inferred from electronic annotation. Source: Ensembl

basal part of cell

Inferred from direct assay PubMed 11767049. Source: MGI

basal plasma membrane

Inferred from direct assay PubMed 11507772PubMed 21464233. Source: MGI

basement membrane

Inferred from direct assay PubMed 9524190. Source: MGI

basolateral plasma membrane

Inferred from direct assay PubMed 11891657. Source: MGI

cell surface

Inferred from direct assay PubMed 1833411PubMed 8306881. Source: MGI

cell-cell adherens junction

Inferred from electronic annotation. Source: Ensembl

external side of plasma membrane

Inferred from direct assay PubMed 19303854PubMed 20103531PubMed 8557754PubMed 9553049. Source: MGI

filopodium

Inferred from electronic annotation. Source: Ensembl

hemidesmosome

Inferred from direct assay PubMed 22274697. Source: MGI

integrin complex

Inferred from direct assay PubMed 8707838. Source: MGI

plasma membrane

Inferred from direct assay PubMed 11891657PubMed 12591243PubMed 12670870PubMed 16510873PubMed 8409377. Source: MGI

   Molecular_functionintegrin binding

Inferred from physical interaction PubMed 1833411. Source: MGI

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform Alpha-6X1B (identifier: Q61739-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Alpha-6X1A (identifier: Q61739-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1045-1091: SRYDDSIPRY...TKWNENESYS → NKKDHYDATYHKAEIHTQPSDKERLTSDA

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 By similarity
Chain24 – 10911068Integrin alpha-6
PRO_0000016261
Chain24 – 899876Integrin alpha-6 heavy chain Potential
PRO_0000016262
Chain903 – 1091189Integrin alpha-6 light chain Potential
PRO_0000016263

Regions

Topological domain24 – 1011988Extracellular Potential
Transmembrane1012 – 103726Helical; Potential
Topological domain1038 – 109154Cytoplasmic Potential
Repeat30 – 9566FG-GAP 1
Repeat101 – 16666FG-GAP 2
Repeat176 – 22954FG-GAP 3
Repeat244 – 30158FG-GAP 4
Repeat302 – 36362FG-GAP 5
Repeat364 – 41956FG-GAP 6
Repeat420 – 47960FG-GAP 7
Calcium binding324 – 3329 Potential
Calcium binding386 – 3949 Potential
Calcium binding441 – 4499 Potential
Motif1040 – 10445GFFKR motif

Amino acid modifications

Lipidation10391S-palmitoyl cysteine; by DHHC3 By similarity
Glycosylation781N-linked (GlcNAc...) Potential
Glycosylation2231N-linked (GlcNAc...) Potential
Glycosylation2841N-linked (GlcNAc...) Ref.3
Glycosylation3701N-linked (GlcNAc...) Potential
Glycosylation7311N-linked (GlcNAc...) Potential
Glycosylation7461N-linked (GlcNAc...) Potential
Glycosylation9271N-linked (GlcNAc...) Ref.3
Glycosylation9581N-linked (GlcNAc...) Ref.3
Disulfide bond86 ↔ 94 By similarity
Disulfide bond131 ↔ 154 By similarity
Disulfide bond175 ↔ 188 By similarity
Disulfide bond489 ↔ 496 By similarity
Disulfide bond502 ↔ 562 By similarity
Disulfide bond626 ↔ 632 By similarity
Disulfide bond726 ↔ 737 By similarity
Disulfide bond881 ↔ 928Interchain (between heavy and light chains) By similarity
Disulfide bond934 ↔ 939 By similarity

Natural variations

Alternative sequence1045 – 109147SRYDD…NESYS → NKKDHYDATYHKAEIHTQPS DKERLTSDA in isoform Alpha-6X1A.
VSP_002726

Experimental info

Sequence conflict5921S → T in CAA49527. Ref.1
Sequence conflict7811N → K in CAA49527. Ref.1
Sequence conflict7991V → L in CAA49527. Ref.1
Sequence conflict9461S → T in CAA49527. Ref.1
Sequence conflict9531R → C in CAA49527. Ref.1
Sequence conflict9751L → V in CAA49527. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform Alpha-6X1B [UniParc].

Last modified July 27, 2011. Version 3.
Checksum: F33B055C2E8BAFAD

FASTA1,091122,159
        10         20         30         40         50         60 
MAVAGQLCLL YLSAGLLARL GTAFNLDTRE DNVIRKSGDP GSLFGFSLAM HWQLQPEDKR 

        70         80         90        100        110        120 
LLLVGAPRAE ALPLQRANRT GGLYSCDITS RGPCTRIEFD NDADPMSESK EDQWMGVTVQ 

       130        140        150        160        170        180 
SQGPGGKVVT CAHRYEKRQH VNTKQESRDI FGRCYVLSQN LRIEDDMDGG DWSFCDGRLR 

       190        200        210        220        230        240 
GHEKFGSCQQ GVAATFTKDF HYIVFGAPGT YNWKGIVRVE QKNNTFFDMN IFEDGPYEVG 

       250        260        270        280        290        300 
GETDHDESLV PVPANSYLGF SLDSGKGIVS KDDITFVSGA PRANHSGAVV LLKRDMKSAH 

       310        320        330        340        350        360 
LLPEYIFDGE GLASSFGYDV AVVDLNADGW QDIVIGAPQY FDRDGEVGGA VYVYINQQGK 

       370        380        390        400        410        420 
WSNVKPIRLN GTKDSMFGIS VKNIGDINQD GYPDIAVGAP YDDLGKVFIY HGSPTGIITK 

       430        440        450        460        470        480 
PTQVLEGTSP YFGYSIAGNM DLDRNSYPDL AVGSLSDSVT IFRSRPVINI LKTITVTPNR 

       490        500        510        520        530        540 
IDLRQKSMCG SPSGICLKVK ACFEYTAKPS GYNPPISILG ILEAEKERRK SGLSSRVQFR 

       550        560        570        580        590        600 
NQGSEPKYTQ ELTLNRQKQR ACMEETLWLQ ENIRDKLRPI PITASVEIQE PSSRRRVNSL 

       610        620        630        640        650        660 
PEVLPILNSN EAKTVQTDVH FLKEGCGDDN VCNSNLKLEY KFGTREGNQD KFSYLPIQKG 

       670        680        690        700        710        720 
IPELVLKDQK DIALEITVTN SPSDPRNPRK DGDDAHEAKL IATFPDTLTY SAYRELRAFP 

       730        740        750        760        770        780 
EKQLSCVANQ NGSQADCELG NPFKRNSSVT FYLILSTTEV TFDTTDLDIN LKLETTSNQD 

       790        800        810        820        830        840 
NLAPITAKAK VVIELLLSVS GVAKPSQVYF GGTVVGEQAM KSEDEVGSLI EYEFRVINLG 

       850        860        870        880        890        900 
KPLKNLGTAT LNIQWPKEIS NGKWLLYLMK VESKGLEQIV CEPHNEINYL KLKESHNSRK 

       910        920        930        940        950        960 
KRELPEKQID DSRKFSLFPE RKYQTLNCSV NVRCVNIRCP LRGLDSKASL VLRSRLWNST 

       970        980        990       1000       1010       1020 
FLEEYSKLNY LDILLRASID VTAAAQNIKL PHAGTQVRVT VFPSKTVAQY SGVAWWIILL 

      1030       1040       1050       1060       1070       1080 
AVLAGILMLA LLVFLLWKCG FFKRSRYDDS IPRYHAVRIR KEEREIKDEK HMDNLEKKQW 

      1090 
ITKWNENESY S 

« Hide

Isoform Alpha-6X1A [UniParc].

Checksum: C128E9EE14170218
Show »

FASTA1,073119,606

References

« Hide 'large scale' references
[1]"Variants of the alpha 6 beta 1 laminin receptor in early murine development: distribution, molecular cloning and chromosomal localization of the mouse integrin alpha 6 subunit."
Hierck B.P., Thorsteinsdottir S., Niessen C.M., Freund E., van Iperen L., Feyen A., Hogervorst F., Poelmann R.E., Mummery C.L., Sonnenberg A.
Cell Adhes. Commun. 1:33-53(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS ALPHA-6X1A AND ALPHA-6X1B).
Strain: BALB/c.
Tissue: Mammary gland.
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[3]"Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-284; ASN-927 AND ASN-958.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X69902 mRNA. Translation: CAA49527.1.
AL928963 Genomic DNA. Translation: CAM26370.1.
CCDSCCDS16118.1. [Q61739-2]
CCDS71074.1. [Q61739-1]
PIRA40463.
RefSeqNP_001264899.1. NM_001277970.1. [Q61739-1]
XP_006498868.1. XM_006498805.1. [Q61739-1]
UniGeneMm.225096.

3D structure databases

ProteinModelPortalQ61739.
SMRQ61739. Positions 24-591, 1013-1048.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ61739. 1 interaction.
MINTMINT-225133.
STRING10090.ENSMUSP00000040155.

PTM databases

PhosphoSiteQ61739.

Proteomic databases

MaxQBQ61739.
PaxDbQ61739.
PRIDEQ61739.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000028522; ENSMUSP00000028522; ENSMUSG00000027111. [Q61739-2]
ENSMUST00000112101; ENSMUSP00000107729; ENSMUSG00000027111. [Q61739-1]
GeneID16403.
KEGGmmu:16403.
UCSCuc008kbd.1. mouse. [Q61739-1]

Organism-specific databases

CTD3655.
MGIMGI:96605. Itga6.

Phylogenomic databases

eggNOGNOG26407.
GeneTreeENSGT00730000110529.
HOGENOMHOG000015786.
HOVERGENHBG108011.
InParanoidA2AU04.
KOK06485.
OMAVYINQQG.
OrthoDBEOG7K3TK7.
TreeFamTF105391.

Gene expression databases

ArrayExpressQ61739.
BgeeQ61739.
CleanExMM_ITGA6.
GenevestigatorQ61739.

Family and domain databases

InterProIPR013519. Int_alpha_beta-p.
IPR000413. Integrin_alpha.
IPR013649. Integrin_alpha-2.
IPR018184. Integrin_alpha_C_CS.
[Graphical view]
PfamPF08441. Integrin_alpha2. 1 hit.
[Graphical view]
PRINTSPR01185. INTEGRINA.
SMARTSM00191. Int_alpha. 5 hits.
[Graphical view]
PROSITEPS51470. FG_GAP. 7 hits.
PS00242. INTEGRIN_ALPHA. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROQ61739.
SOURCESearch...

Entry information

Entry nameITA6_MOUSE
AccessionPrimary (citable) accession number: Q61739
Secondary accession number(s): A2AU04
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 130 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot