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Protein

Integrin alpha-6

Gene

Itga6

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Integrin alpha-6/beta-1 is a receptor for laminin on platelets. Integrin alpha-6/beta-4 is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome (PubMed:8673141). ITGA6:ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling. ITGA6:ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling (By similarity).By similarity1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi324 – 332Sequence analysis9
Calcium bindingi386 – 394Sequence analysis9
Calcium bindingi441 – 449Sequence analysis9

GO - Molecular functioni

  • cadherin binding involved in cell-cell adhesion Source: MGI
  • integrin binding Source: MGI
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Integrin, Receptor

Keywords - Biological processi

Cell adhesion

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-2022090. Assembly of collagen fibrils and other multimeric structures.
R-MMU-210991. Basigin interactions.
R-MMU-216083. Integrin cell surface interactions.
R-MMU-3000157. Laminin interactions.
R-MMU-446107. Type I hemidesmosome assembly.

Names & Taxonomyi

Protein namesi
Recommended name:
Integrin alpha-6
Alternative name(s):
CD49 antigen-like family member F
VLA-6
CD_antigen: CD49f
Cleaved into the following 2 chains:
Gene namesi
Name:Itga6
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:96605. Itga6.

Subcellular locationi

  • Cell membrane By similarity; Single-pass type I membrane protein Sequence analysis
  • Cell membrane By similarity; Lipid-anchor By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini24 – 1011ExtracellularSequence analysisAdd BLAST988
Transmembranei1012 – 1037HelicalSequence analysisAdd BLAST26
Topological domaini1038 – 1091CytoplasmicSequence analysisAdd BLAST54

GO - Cellular componenti

  • basal part of cell Source: MGI
  • basal plasma membrane Source: MGI
  • basement membrane Source: MGI
  • basolateral plasma membrane Source: MGI
  • cell-cell adherens junction Source: MGI
  • cell surface Source: MGI
  • external side of plasma membrane Source: MGI
  • filopodium Source: Ensembl
  • focal adhesion Source: MGI
  • hemidesmosome Source: MGI
  • integrin alpha6-beta4 complex Source: Ensembl
  • integrin complex Source: MGI
  • plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice expressing a null mutation of the alpha-6 subunit gene die soon after birth and develop severe blistering. The blisters are due to separation of the basal epithelial cells from a normally formed basement membrane.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23By similarityAdd BLAST23
ChainiPRO_000001626124 – 1091Integrin alpha-6Add BLAST1068
ChainiPRO_000001626224 – 899Integrin alpha-6 heavy chainSequence analysisAdd BLAST876
ChainiPRO_0000016263903 – 1091Integrin alpha-6 light chainSequence analysisAdd BLAST189

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi78N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi86 ↔ 94By similarity
Disulfide bondi131 ↔ 154By similarity
Disulfide bondi175 ↔ 188By similarity
Glycosylationi223N-linked (GlcNAc...)Sequence analysis1
Glycosylationi284N-linked (GlcNAc...)1 Publication1
Glycosylationi370N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi489 ↔ 496By similarity
Disulfide bondi502 ↔ 562By similarity
Disulfide bondi626 ↔ 632By similarity
Disulfide bondi726 ↔ 737By similarity
Glycosylationi731N-linked (GlcNAc...)Sequence analysis1
Glycosylationi746N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi881 ↔ 928Interchain (between heavy and light chains)By similarity
Glycosylationi927N-linked (GlcNAc...)1 Publication1
Disulfide bondi934 ↔ 939By similarity
Glycosylationi958N-linked (GlcNAc...)1 Publication1
Lipidationi1039S-palmitoyl cysteine; by DHHC3By similarity1

Post-translational modificationi

Isoforms containing segment A, but not segment B, are the major targets for PMA-induced phosphorylation. Phosphorylation occurs on 'Ser-1064' of isoform alpha-6X1A. Phosphorylation is not required for the induction of integrin alpha-6A/beta-1 high affinity but may reduce the affinity for ligand (By similarity).By similarity
Palmitoylation by DHHC3 enhances stability and cell surface expression.By similarity

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiQ61739.
PeptideAtlasiQ61739.
PRIDEiQ61739.

PTM databases

iPTMnetiQ61739.
PhosphoSitePlusiQ61739.
SwissPalmiQ61739.

Expressioni

Gene expression databases

BgeeiENSMUSG00000027111.
CleanExiMM_ITGA6.
ExpressionAtlasiQ61739. baseline and differential.
GenevisibleiQ61739. MM.

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of a heavy and a light chain linked by a disulfide bond. Alpha-6 associates with either beta-1 or beta-4. Interacts with RAB21. ITGA6:ITGB4 is found in a ternary complex with NRG1 and ERBB3. ITGA6:ITGB4 is found in a ternary complex with IGF1 and IGF1R (By similarity). Interacts with ADAM9 (PubMed:10825303).By similarity1 Publication

GO - Molecular functioni

  • cadherin binding involved in cell-cell adhesion Source: MGI
  • integrin binding Source: MGI

Protein-protein interaction databases

BioGridi200819. 3 interactors.
IntActiQ61739. 1 interactor.
MINTiMINT-225133.
STRINGi10090.ENSMUSP00000028522.

Structurei

3D structure databases

ProteinModelPortaliQ61739.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati30 – 95FG-GAP 1PROSITE-ProRule annotationAdd BLAST66
Repeati101 – 166FG-GAP 2PROSITE-ProRule annotationAdd BLAST66
Repeati176 – 229FG-GAP 3PROSITE-ProRule annotationAdd BLAST54
Repeati244 – 300FG-GAP 4PROSITE-ProRule annotationAdd BLAST57
Repeati301 – 363FG-GAP 5PROSITE-ProRule annotationAdd BLAST63
Repeati364 – 419FG-GAP 6PROSITE-ProRule annotationAdd BLAST56
Repeati420 – 479FG-GAP 7PROSITE-ProRule annotationAdd BLAST60

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi1040 – 1044GFFKR motif5

Sequence similaritiesi

Belongs to the integrin alpha chain family.Curated
Contains 7 FG-GAP repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3637. Eukaryota.
ENOG410XPVZ. LUCA.
GeneTreeiENSGT00760000118782.
HOGENOMiHOG000015786.
HOVERGENiHBG108011.
InParanoidiQ61739.
KOiK06485.
OMAiINQQGRW.
OrthoDBiEOG091G012D.
TreeFamiTF105391.

Family and domain databases

InterProiIPR013519. Int_alpha_beta-p.
IPR000413. Integrin_alpha.
IPR013649. Integrin_alpha-2.
IPR018184. Integrin_alpha_C_CS.
IPR032695. Integrin_dom.
[Graphical view]
PfamiPF08441. Integrin_alpha2. 1 hit.
[Graphical view]
PRINTSiPR01185. INTEGRINA.
SMARTiSM00191. Int_alpha. 5 hits.
[Graphical view]
SUPFAMiSSF69179. SSF69179. 3 hits.
PROSITEiPS51470. FG_GAP. 7 hits.
PS00242. INTEGRIN_ALPHA. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Alpha-6X1B (identifier: Q61739-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAVAGQLCLL YLSAGLLARL GTAFNLDTRE DNVIRKSGDP GSLFGFSLAM
60 70 80 90 100
HWQLQPEDKR LLLVGAPRAE ALPLQRANRT GGLYSCDITS RGPCTRIEFD
110 120 130 140 150
NDADPMSESK EDQWMGVTVQ SQGPGGKVVT CAHRYEKRQH VNTKQESRDI
160 170 180 190 200
FGRCYVLSQN LRIEDDMDGG DWSFCDGRLR GHEKFGSCQQ GVAATFTKDF
210 220 230 240 250
HYIVFGAPGT YNWKGIVRVE QKNNTFFDMN IFEDGPYEVG GETDHDESLV
260 270 280 290 300
PVPANSYLGF SLDSGKGIVS KDDITFVSGA PRANHSGAVV LLKRDMKSAH
310 320 330 340 350
LLPEYIFDGE GLASSFGYDV AVVDLNADGW QDIVIGAPQY FDRDGEVGGA
360 370 380 390 400
VYVYINQQGK WSNVKPIRLN GTKDSMFGIS VKNIGDINQD GYPDIAVGAP
410 420 430 440 450
YDDLGKVFIY HGSPTGIITK PTQVLEGTSP YFGYSIAGNM DLDRNSYPDL
460 470 480 490 500
AVGSLSDSVT IFRSRPVINI LKTITVTPNR IDLRQKSMCG SPSGICLKVK
510 520 530 540 550
ACFEYTAKPS GYNPPISILG ILEAEKERRK SGLSSRVQFR NQGSEPKYTQ
560 570 580 590 600
ELTLNRQKQR ACMEETLWLQ ENIRDKLRPI PITASVEIQE PSSRRRVNSL
610 620 630 640 650
PEVLPILNSN EAKTVQTDVH FLKEGCGDDN VCNSNLKLEY KFGTREGNQD
660 670 680 690 700
KFSYLPIQKG IPELVLKDQK DIALEITVTN SPSDPRNPRK DGDDAHEAKL
710 720 730 740 750
IATFPDTLTY SAYRELRAFP EKQLSCVANQ NGSQADCELG NPFKRNSSVT
760 770 780 790 800
FYLILSTTEV TFDTTDLDIN LKLETTSNQD NLAPITAKAK VVIELLLSVS
810 820 830 840 850
GVAKPSQVYF GGTVVGEQAM KSEDEVGSLI EYEFRVINLG KPLKNLGTAT
860 870 880 890 900
LNIQWPKEIS NGKWLLYLMK VESKGLEQIV CEPHNEINYL KLKESHNSRK
910 920 930 940 950
KRELPEKQID DSRKFSLFPE RKYQTLNCSV NVRCVNIRCP LRGLDSKASL
960 970 980 990 1000
VLRSRLWNST FLEEYSKLNY LDILLRASID VTAAAQNIKL PHAGTQVRVT
1010 1020 1030 1040 1050
VFPSKTVAQY SGVAWWIILL AVLAGILMLA LLVFLLWKCG FFKRSRYDDS
1060 1070 1080 1090
IPRYHAVRIR KEEREIKDEK HMDNLEKKQW ITKWNENESY S
Length:1,091
Mass (Da):122,159
Last modified:July 27, 2011 - v3
Checksum:iF33B055C2E8BAFAD
GO
Isoform Alpha-6X1A (identifier: Q61739-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1045-1091: SRYDDSIPRY...TKWNENESYS → NKKDHYDATYHKAEIHTQPSDKERLTSDA

Show »
Length:1,073
Mass (Da):119,606
Checksum:iC128E9EE14170218
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti592S → T in CAA49527 (PubMed:8081870).Curated1
Sequence conflicti781N → K in CAA49527 (PubMed:8081870).Curated1
Sequence conflicti799V → L in CAA49527 (PubMed:8081870).Curated1
Sequence conflicti946S → T in CAA49527 (PubMed:8081870).Curated1
Sequence conflicti953R → C in CAA49527 (PubMed:8081870).Curated1
Sequence conflicti975L → V in CAA49527 (PubMed:8081870).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0027261045 – 1091SRYDD…NESYS → NKKDHYDATYHKAEIHTQPS DKERLTSDA in isoform Alpha-6X1A. 1 PublicationAdd BLAST47

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69902 mRNA. Translation: CAA49527.1.
AL928963 Genomic DNA. Translation: CAM26370.1.
CCDSiCCDS16118.1. [Q61739-2]
CCDS71074.1. [Q61739-1]
PIRiA40463.
RefSeqiNP_001264899.1. NM_001277970.1. [Q61739-1]
XP_011237610.1. XM_011239308.1. [Q61739-1]
UniGeneiMm.225096.

Genome annotation databases

EnsembliENSMUST00000028522; ENSMUSP00000028522; ENSMUSG00000027111. [Q61739-2]
ENSMUST00000112101; ENSMUSP00000107729; ENSMUSG00000027111. [Q61739-1]
GeneIDi16403.
KEGGimmu:16403.
UCSCiuc008kbd.2. mouse. [Q61739-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69902 mRNA. Translation: CAA49527.1.
AL928963 Genomic DNA. Translation: CAM26370.1.
CCDSiCCDS16118.1. [Q61739-2]
CCDS71074.1. [Q61739-1]
PIRiA40463.
RefSeqiNP_001264899.1. NM_001277970.1. [Q61739-1]
XP_011237610.1. XM_011239308.1. [Q61739-1]
UniGeneiMm.225096.

3D structure databases

ProteinModelPortaliQ61739.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200819. 3 interactors.
IntActiQ61739. 1 interactor.
MINTiMINT-225133.
STRINGi10090.ENSMUSP00000028522.

PTM databases

iPTMnetiQ61739.
PhosphoSitePlusiQ61739.
SwissPalmiQ61739.

Proteomic databases

PaxDbiQ61739.
PeptideAtlasiQ61739.
PRIDEiQ61739.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000028522; ENSMUSP00000028522; ENSMUSG00000027111. [Q61739-2]
ENSMUST00000112101; ENSMUSP00000107729; ENSMUSG00000027111. [Q61739-1]
GeneIDi16403.
KEGGimmu:16403.
UCSCiuc008kbd.2. mouse. [Q61739-1]

Organism-specific databases

CTDi3655.
MGIiMGI:96605. Itga6.

Phylogenomic databases

eggNOGiKOG3637. Eukaryota.
ENOG410XPVZ. LUCA.
GeneTreeiENSGT00760000118782.
HOGENOMiHOG000015786.
HOVERGENiHBG108011.
InParanoidiQ61739.
KOiK06485.
OMAiINQQGRW.
OrthoDBiEOG091G012D.
TreeFamiTF105391.

Enzyme and pathway databases

ReactomeiR-MMU-2022090. Assembly of collagen fibrils and other multimeric structures.
R-MMU-210991. Basigin interactions.
R-MMU-216083. Integrin cell surface interactions.
R-MMU-3000157. Laminin interactions.
R-MMU-446107. Type I hemidesmosome assembly.

Miscellaneous databases

PROiQ61739.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027111.
CleanExiMM_ITGA6.
ExpressionAtlasiQ61739. baseline and differential.
GenevisibleiQ61739. MM.

Family and domain databases

InterProiIPR013519. Int_alpha_beta-p.
IPR000413. Integrin_alpha.
IPR013649. Integrin_alpha-2.
IPR018184. Integrin_alpha_C_CS.
IPR032695. Integrin_dom.
[Graphical view]
PfamiPF08441. Integrin_alpha2. 1 hit.
[Graphical view]
PRINTSiPR01185. INTEGRINA.
SMARTiSM00191. Int_alpha. 5 hits.
[Graphical view]
SUPFAMiSSF69179. SSF69179. 3 hits.
PROSITEiPS51470. FG_GAP. 7 hits.
PS00242. INTEGRIN_ALPHA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiITA6_MOUSE
AccessioniPrimary (citable) accession number: Q61739
Secondary accession number(s): A2AU04
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 27, 2011
Last modified: November 30, 2016
This is version 152 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.