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Q61739

- ITA6_MOUSE

UniProt

Q61739 - ITA6_MOUSE

Protein

Integrin alpha-6

Gene

Itga6

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 132 (01 Oct 2014)
      Sequence version 3 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Integrin alpha-6/beta-1 is a receptor for laminin on platelets. Integrin alpha-6/beta-4 is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome. Mice expressing a null mutation of the alpha-6 subunit gene die soon after birth and develop severe blistering. The blisters are due to separation of the basal epithelial cells from a normally formed basement membrane.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Calcium bindingi324 – 3329Sequence Analysis
    Calcium bindingi386 – 3949Sequence Analysis
    Calcium bindingi441 – 4499Sequence Analysis

    GO - Molecular functioni

    1. integrin binding Source: MGI
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. brown fat cell differentiation Source: MGI
    2. cell adhesion mediated by integrin Source: MGI
    3. cell-matrix adhesion Source: MGI
    4. cellular response to extracellular stimulus Source: MGI
    5. cellular response to organic cyclic compound Source: Ensembl
    6. filopodium assembly Source: MGI
    7. integrin-mediated signaling pathway Source: UniProtKB-KW
    8. leukocyte migration Source: MGI
    9. negative regulation of extrinsic apoptotic signaling pathway Source: Ensembl
    10. odontogenesis of dentin-containing tooth Source: MGI
    11. positive regulation of apoptotic process Source: Ensembl
    12. positive regulation of cell-cell adhesion Source: MGI
    13. positive regulation of cell-substrate adhesion Source: Ensembl
    14. positive regulation of phosphorylation Source: Ensembl
    15. positive regulation of transcription from RNA polymerase II promoter Source: Ensembl
    16. single organismal cell-cell adhesion Source: Ensembl

    Keywords - Molecular functioni

    Integrin, Receptor

    Keywords - Biological processi

    Cell adhesion

    Keywords - Ligandi

    Calcium, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_202342. Laminin interactions.
    REACT_211508. Basigin interactions.
    REACT_216309. Integrin cell surface interactions.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Integrin alpha-6
    Alternative name(s):
    CD49 antigen-like family member F
    VLA-6
    CD_antigen: CD49f
    Cleaved into the following 2 chains:
    Gene namesi
    Name:Itga6
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:96605. Itga6.

    Subcellular locationi

    GO - Cellular componenti

    1. basal part of cell Source: MGI
    2. basal plasma membrane Source: MGI
    3. basement membrane Source: MGI
    4. basolateral plasma membrane Source: MGI
    5. cell-cell adherens junction Source: Ensembl
    6. cell surface Source: MGI
    7. external side of plasma membrane Source: MGI
    8. filopodium Source: Ensembl
    9. hemidesmosome Source: MGI
    10. integrin alpha6-beta4 complex Source: Ensembl
    11. integrin complex Source: MGI
    12. plasma membrane Source: MGI

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2323By similarityAdd
    BLAST
    Chaini24 – 10911068Integrin alpha-6PRO_0000016261Add
    BLAST
    Chaini24 – 899876Integrin alpha-6 heavy chainSequence AnalysisPRO_0000016262Add
    BLAST
    Chaini903 – 1091189Integrin alpha-6 light chainSequence AnalysisPRO_0000016263Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi78 – 781N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi86 ↔ 94By similarity
    Disulfide bondi131 ↔ 154By similarity
    Disulfide bondi175 ↔ 188By similarity
    Glycosylationi223 – 2231N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi284 – 2841N-linked (GlcNAc...)1 Publication
    Glycosylationi370 – 3701N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi489 ↔ 496By similarity
    Disulfide bondi502 ↔ 562By similarity
    Disulfide bondi626 ↔ 632By similarity
    Disulfide bondi726 ↔ 737By similarity
    Glycosylationi731 – 7311N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi746 – 7461N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi881 ↔ 928Interchain (between heavy and light chains)By similarity
    Glycosylationi927 – 9271N-linked (GlcNAc...)1 Publication
    Disulfide bondi934 ↔ 939By similarity
    Glycosylationi958 – 9581N-linked (GlcNAc...)1 Publication
    Lipidationi1039 – 10391S-palmitoyl cysteine; by DHHC3By similarity

    Post-translational modificationi

    Isoforms containing segment A, but not segment B, are the major targets for PMA-induced phosphorylation. Phosphorylation occurs on 'Ser-1064' of isoform alpha-6X1A. Phosphorylation is not required for the induction of integrin alpha-6A/beta-1 high affinity but may reduce the affinity for ligand By similarity.By similarity
    Palmitoylation by DHHC3 enhances stability and cell surface expression.By similarity

    Keywords - PTMi

    Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

    Proteomic databases

    MaxQBiQ61739.
    PaxDbiQ61739.
    PRIDEiQ61739.

    PTM databases

    PhosphoSiteiQ61739.

    Expressioni

    Gene expression databases

    ArrayExpressiQ61739.
    BgeeiQ61739.
    CleanExiMM_ITGA6.
    GenevestigatoriQ61739.

    Interactioni

    Subunit structurei

    Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of a heavy and a light chain linked by a disulfide bond. Alpha-6 associates with either beta-1 or beta-4. Interacts with RAB21 By similarity.By similarity

    Protein-protein interaction databases

    IntActiQ61739. 1 interaction.
    MINTiMINT-225133.
    STRINGi10090.ENSMUSP00000040155.

    Structurei

    3D structure databases

    ProteinModelPortaliQ61739.
    SMRiQ61739. Positions 24-591, 1013-1048.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini24 – 1011988ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini1038 – 109154CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei1012 – 103726HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati30 – 9566FG-GAP 1Add
    BLAST
    Repeati101 – 16666FG-GAP 2Add
    BLAST
    Repeati176 – 22954FG-GAP 3Add
    BLAST
    Repeati244 – 30158FG-GAP 4Add
    BLAST
    Repeati302 – 36362FG-GAP 5Add
    BLAST
    Repeati364 – 41956FG-GAP 6Add
    BLAST
    Repeati420 – 47960FG-GAP 7Add
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi1040 – 10445GFFKR motif

    Sequence similaritiesi

    Belongs to the integrin alpha chain family.Curated
    Contains 7 FG-GAP repeats.Curated

    Keywords - Domaini

    Repeat, Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG26407.
    GeneTreeiENSGT00730000110529.
    HOGENOMiHOG000015786.
    HOVERGENiHBG108011.
    InParanoidiA2AU04.
    KOiK06485.
    OMAiVYINQQG.
    OrthoDBiEOG7K3TK7.
    TreeFamiTF105391.

    Family and domain databases

    InterProiIPR013519. Int_alpha_beta-p.
    IPR000413. Integrin_alpha.
    IPR013649. Integrin_alpha-2.
    IPR018184. Integrin_alpha_C_CS.
    [Graphical view]
    PfamiPF08441. Integrin_alpha2. 1 hit.
    [Graphical view]
    PRINTSiPR01185. INTEGRINA.
    SMARTiSM00191. Int_alpha. 5 hits.
    [Graphical view]
    PROSITEiPS51470. FG_GAP. 7 hits.
    PS00242. INTEGRIN_ALPHA. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform Alpha-6X1B (identifier: Q61739-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAVAGQLCLL YLSAGLLARL GTAFNLDTRE DNVIRKSGDP GSLFGFSLAM     50
    HWQLQPEDKR LLLVGAPRAE ALPLQRANRT GGLYSCDITS RGPCTRIEFD 100
    NDADPMSESK EDQWMGVTVQ SQGPGGKVVT CAHRYEKRQH VNTKQESRDI 150
    FGRCYVLSQN LRIEDDMDGG DWSFCDGRLR GHEKFGSCQQ GVAATFTKDF 200
    HYIVFGAPGT YNWKGIVRVE QKNNTFFDMN IFEDGPYEVG GETDHDESLV 250
    PVPANSYLGF SLDSGKGIVS KDDITFVSGA PRANHSGAVV LLKRDMKSAH 300
    LLPEYIFDGE GLASSFGYDV AVVDLNADGW QDIVIGAPQY FDRDGEVGGA 350
    VYVYINQQGK WSNVKPIRLN GTKDSMFGIS VKNIGDINQD GYPDIAVGAP 400
    YDDLGKVFIY HGSPTGIITK PTQVLEGTSP YFGYSIAGNM DLDRNSYPDL 450
    AVGSLSDSVT IFRSRPVINI LKTITVTPNR IDLRQKSMCG SPSGICLKVK 500
    ACFEYTAKPS GYNPPISILG ILEAEKERRK SGLSSRVQFR NQGSEPKYTQ 550
    ELTLNRQKQR ACMEETLWLQ ENIRDKLRPI PITASVEIQE PSSRRRVNSL 600
    PEVLPILNSN EAKTVQTDVH FLKEGCGDDN VCNSNLKLEY KFGTREGNQD 650
    KFSYLPIQKG IPELVLKDQK DIALEITVTN SPSDPRNPRK DGDDAHEAKL 700
    IATFPDTLTY SAYRELRAFP EKQLSCVANQ NGSQADCELG NPFKRNSSVT 750
    FYLILSTTEV TFDTTDLDIN LKLETTSNQD NLAPITAKAK VVIELLLSVS 800
    GVAKPSQVYF GGTVVGEQAM KSEDEVGSLI EYEFRVINLG KPLKNLGTAT 850
    LNIQWPKEIS NGKWLLYLMK VESKGLEQIV CEPHNEINYL KLKESHNSRK 900
    KRELPEKQID DSRKFSLFPE RKYQTLNCSV NVRCVNIRCP LRGLDSKASL 950
    VLRSRLWNST FLEEYSKLNY LDILLRASID VTAAAQNIKL PHAGTQVRVT 1000
    VFPSKTVAQY SGVAWWIILL AVLAGILMLA LLVFLLWKCG FFKRSRYDDS 1050
    IPRYHAVRIR KEEREIKDEK HMDNLEKKQW ITKWNENESY S 1091
    Length:1,091
    Mass (Da):122,159
    Last modified:July 27, 2011 - v3
    Checksum:iF33B055C2E8BAFAD
    GO
    Isoform Alpha-6X1A (identifier: Q61739-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1045-1091: SRYDDSIPRY...TKWNENESYS → NKKDHYDATYHKAEIHTQPSDKERLTSDA

    Show »
    Length:1,073
    Mass (Da):119,606
    Checksum:iC128E9EE14170218
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti592 – 5921S → T in CAA49527. (PubMed:8081870)Curated
    Sequence conflicti781 – 7811N → K in CAA49527. (PubMed:8081870)Curated
    Sequence conflicti799 – 7991V → L in CAA49527. (PubMed:8081870)Curated
    Sequence conflicti946 – 9461S → T in CAA49527. (PubMed:8081870)Curated
    Sequence conflicti953 – 9531R → C in CAA49527. (PubMed:8081870)Curated
    Sequence conflicti975 – 9751L → V in CAA49527. (PubMed:8081870)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1045 – 109147SRYDD…NESYS → NKKDHYDATYHKAEIHTQPS DKERLTSDA in isoform Alpha-6X1A. 1 PublicationVSP_002726Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X69902 mRNA. Translation: CAA49527.1.
    AL928963 Genomic DNA. Translation: CAM26370.1.
    CCDSiCCDS16118.1. [Q61739-2]
    CCDS71074.1. [Q61739-1]
    PIRiA40463.
    RefSeqiNP_001264899.1. NM_001277970.1. [Q61739-1]
    XP_006498868.1. XM_006498805.1. [Q61739-1]
    UniGeneiMm.225096.

    Genome annotation databases

    EnsembliENSMUST00000028522; ENSMUSP00000028522; ENSMUSG00000027111. [Q61739-2]
    ENSMUST00000112101; ENSMUSP00000107729; ENSMUSG00000027111. [Q61739-1]
    GeneIDi16403.
    KEGGimmu:16403.
    UCSCiuc008kbd.1. mouse. [Q61739-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X69902 mRNA. Translation: CAA49527.1 .
    AL928963 Genomic DNA. Translation: CAM26370.1 .
    CCDSi CCDS16118.1. [Q61739-2 ]
    CCDS71074.1. [Q61739-1 ]
    PIRi A40463.
    RefSeqi NP_001264899.1. NM_001277970.1. [Q61739-1 ]
    XP_006498868.1. XM_006498805.1. [Q61739-1 ]
    UniGenei Mm.225096.

    3D structure databases

    ProteinModelPortali Q61739.
    SMRi Q61739. Positions 24-591, 1013-1048.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q61739. 1 interaction.
    MINTi MINT-225133.
    STRINGi 10090.ENSMUSP00000040155.

    PTM databases

    PhosphoSitei Q61739.

    Proteomic databases

    MaxQBi Q61739.
    PaxDbi Q61739.
    PRIDEi Q61739.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000028522 ; ENSMUSP00000028522 ; ENSMUSG00000027111 . [Q61739-2 ]
    ENSMUST00000112101 ; ENSMUSP00000107729 ; ENSMUSG00000027111 . [Q61739-1 ]
    GeneIDi 16403.
    KEGGi mmu:16403.
    UCSCi uc008kbd.1. mouse. [Q61739-1 ]

    Organism-specific databases

    CTDi 3655.
    MGIi MGI:96605. Itga6.

    Phylogenomic databases

    eggNOGi NOG26407.
    GeneTreei ENSGT00730000110529.
    HOGENOMi HOG000015786.
    HOVERGENi HBG108011.
    InParanoidi A2AU04.
    KOi K06485.
    OMAi VYINQQG.
    OrthoDBi EOG7K3TK7.
    TreeFami TF105391.

    Enzyme and pathway databases

    Reactomei REACT_202342. Laminin interactions.
    REACT_211508. Basigin interactions.
    REACT_216309. Integrin cell surface interactions.

    Miscellaneous databases

    PROi Q61739.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q61739.
    Bgeei Q61739.
    CleanExi MM_ITGA6.
    Genevestigatori Q61739.

    Family and domain databases

    InterProi IPR013519. Int_alpha_beta-p.
    IPR000413. Integrin_alpha.
    IPR013649. Integrin_alpha-2.
    IPR018184. Integrin_alpha_C_CS.
    [Graphical view ]
    Pfami PF08441. Integrin_alpha2. 1 hit.
    [Graphical view ]
    PRINTSi PR01185. INTEGRINA.
    SMARTi SM00191. Int_alpha. 5 hits.
    [Graphical view ]
    PROSITEi PS51470. FG_GAP. 7 hits.
    PS00242. INTEGRIN_ALPHA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Variants of the alpha 6 beta 1 laminin receptor in early murine development: distribution, molecular cloning and chromosomal localization of the mouse integrin alpha 6 subunit."
      Hierck B.P., Thorsteinsdottir S., Niessen C.M., Freund E., van Iperen L., Feyen A., Hogervorst F., Poelmann R.E., Mummery C.L., Sonnenberg A.
      Cell Adhes. Commun. 1:33-53(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS ALPHA-6X1A AND ALPHA-6X1B).
      Strain: BALB/c.
      Tissue: Mammary gland.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    3. "Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
      Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
      Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-284; ASN-927 AND ASN-958.

    Entry informationi

    Entry nameiITA6_MOUSE
    AccessioniPrimary (citable) accession number: Q61739
    Secondary accession number(s): A2AU04
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 132 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3