Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q61739

- ITA6_MOUSE

UniProt

Q61739 - ITA6_MOUSE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Integrin alpha-6

Gene

Itga6

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Integrin alpha-6/beta-1 is a receptor for laminin on platelets. Integrin alpha-6/beta-4 is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome. Mice expressing a null mutation of the alpha-6 subunit gene die soon after birth and develop severe blistering. The blisters are due to separation of the basal epithelial cells from a normally formed basement membrane.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi324 – 3329Sequence Analysis
Calcium bindingi386 – 3949Sequence Analysis
Calcium bindingi441 – 4499Sequence Analysis

GO - Molecular functioni

  1. integrin binding Source: MGI
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. brown fat cell differentiation Source: MGI
  2. cell adhesion mediated by integrin Source: MGI
  3. cell-matrix adhesion Source: MGI
  4. cellular response to extracellular stimulus Source: MGI
  5. cellular response to organic cyclic compound Source: Ensembl
  6. filopodium assembly Source: MGI
  7. integrin-mediated signaling pathway Source: UniProtKB-KW
  8. leukocyte migration Source: MGI
  9. negative regulation of extrinsic apoptotic signaling pathway Source: Ensembl
  10. odontogenesis of dentin-containing tooth Source: MGI
  11. positive regulation of apoptotic process Source: Ensembl
  12. positive regulation of cell-cell adhesion Source: MGI
  13. positive regulation of cell-substrate adhesion Source: Ensembl
  14. positive regulation of phosphorylation Source: Ensembl
  15. positive regulation of transcription from RNA polymerase II promoter Source: Ensembl
  16. single organismal cell-cell adhesion Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Integrin, Receptor

Keywords - Biological processi

Cell adhesion

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_199046. Assembly of collagen fibrils and other multimeric structures.
REACT_202342. Laminin interactions.
REACT_211508. Basigin interactions.
REACT_216309. Integrin cell surface interactions.
REACT_262098. Type I hemidesmosome assembly.

Names & Taxonomyi

Protein namesi
Recommended name:
Integrin alpha-6
Alternative name(s):
CD49 antigen-like family member F
VLA-6
CD_antigen: CD49f
Cleaved into the following 2 chains:
Gene namesi
Name:Itga6
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 2

Organism-specific databases

MGIiMGI:96605. Itga6.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini24 – 1011988ExtracellularSequence AnalysisAdd
BLAST
Transmembranei1012 – 103726HelicalSequence AnalysisAdd
BLAST
Topological domaini1038 – 109154CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. basal part of cell Source: MGI
  2. basal plasma membrane Source: MGI
  3. basement membrane Source: MGI
  4. basolateral plasma membrane Source: MGI
  5. cell-cell adherens junction Source: Ensembl
  6. cell surface Source: MGI
  7. external side of plasma membrane Source: MGI
  8. filopodium Source: Ensembl
  9. hemidesmosome Source: MGI
  10. integrin alpha6-beta4 complex Source: Ensembl
  11. integrin complex Source: MGI
  12. plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323By similarityAdd
BLAST
Chaini24 – 10911068Integrin alpha-6PRO_0000016261Add
BLAST
Chaini24 – 899876Integrin alpha-6 heavy chainSequence AnalysisPRO_0000016262Add
BLAST
Chaini903 – 1091189Integrin alpha-6 light chainSequence AnalysisPRO_0000016263Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi78 – 781N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi86 ↔ 94By similarity
Disulfide bondi131 ↔ 154By similarity
Disulfide bondi175 ↔ 188By similarity
Glycosylationi223 – 2231N-linked (GlcNAc...)Sequence Analysis
Glycosylationi284 – 2841N-linked (GlcNAc...)1 Publication
Glycosylationi370 – 3701N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi489 ↔ 496By similarity
Disulfide bondi502 ↔ 562By similarity
Disulfide bondi626 ↔ 632By similarity
Disulfide bondi726 ↔ 737By similarity
Glycosylationi731 – 7311N-linked (GlcNAc...)Sequence Analysis
Glycosylationi746 – 7461N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi881 ↔ 928Interchain (between heavy and light chains)By similarity
Glycosylationi927 – 9271N-linked (GlcNAc...)1 Publication
Disulfide bondi934 ↔ 939By similarity
Glycosylationi958 – 9581N-linked (GlcNAc...)1 Publication
Lipidationi1039 – 10391S-palmitoyl cysteine; by DHHC3By similarity

Post-translational modificationi

Isoforms containing segment A, but not segment B, are the major targets for PMA-induced phosphorylation. Phosphorylation occurs on 'Ser-1064' of isoform alpha-6X1A. Phosphorylation is not required for the induction of integrin alpha-6A/beta-1 high affinity but may reduce the affinity for ligand (By similarity).By similarity
Palmitoylation by DHHC3 enhances stability and cell surface expression.By similarity

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

MaxQBiQ61739.
PaxDbiQ61739.
PRIDEiQ61739.

PTM databases

PhosphoSiteiQ61739.

Expressioni

Gene expression databases

BgeeiQ61739.
CleanExiMM_ITGA6.
ExpressionAtlasiQ61739. baseline and differential.
GenevestigatoriQ61739.

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of a heavy and a light chain linked by a disulfide bond. Alpha-6 associates with either beta-1 or beta-4. Interacts with RAB21 (By similarity).By similarity

Protein-protein interaction databases

IntActiQ61739. 1 interaction.
MINTiMINT-225133.
STRINGi10090.ENSMUSP00000040155.

Structurei

3D structure databases

ProteinModelPortaliQ61739.
SMRiQ61739. Positions 24-841.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati30 – 9566FG-GAP 1Add
BLAST
Repeati101 – 16666FG-GAP 2Add
BLAST
Repeati176 – 22954FG-GAP 3Add
BLAST
Repeati244 – 30158FG-GAP 4Add
BLAST
Repeati302 – 36362FG-GAP 5Add
BLAST
Repeati364 – 41956FG-GAP 6Add
BLAST
Repeati420 – 47960FG-GAP 7Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi1040 – 10445GFFKR motif

Sequence similaritiesi

Belongs to the integrin alpha chain family.Curated
Contains 7 FG-GAP repeats.Curated

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG26407.
GeneTreeiENSGT00760000118782.
HOGENOMiHOG000015786.
HOVERGENiHBG108011.
InParanoidiQ61739.
KOiK06485.
OMAiVYINQQG.
OrthoDBiEOG7K3TK7.
TreeFamiTF105391.

Family and domain databases

InterProiIPR013519. Int_alpha_beta-p.
IPR000413. Integrin_alpha.
IPR013649. Integrin_alpha-2.
IPR018184. Integrin_alpha_C_CS.
[Graphical view]
PfamiPF08441. Integrin_alpha2. 1 hit.
[Graphical view]
PRINTSiPR01185. INTEGRINA.
SMARTiSM00191. Int_alpha. 5 hits.
[Graphical view]
PROSITEiPS51470. FG_GAP. 7 hits.
PS00242. INTEGRIN_ALPHA. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform Alpha-6X1B (identifier: Q61739-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAVAGQLCLL YLSAGLLARL GTAFNLDTRE DNVIRKSGDP GSLFGFSLAM
60 70 80 90 100
HWQLQPEDKR LLLVGAPRAE ALPLQRANRT GGLYSCDITS RGPCTRIEFD
110 120 130 140 150
NDADPMSESK EDQWMGVTVQ SQGPGGKVVT CAHRYEKRQH VNTKQESRDI
160 170 180 190 200
FGRCYVLSQN LRIEDDMDGG DWSFCDGRLR GHEKFGSCQQ GVAATFTKDF
210 220 230 240 250
HYIVFGAPGT YNWKGIVRVE QKNNTFFDMN IFEDGPYEVG GETDHDESLV
260 270 280 290 300
PVPANSYLGF SLDSGKGIVS KDDITFVSGA PRANHSGAVV LLKRDMKSAH
310 320 330 340 350
LLPEYIFDGE GLASSFGYDV AVVDLNADGW QDIVIGAPQY FDRDGEVGGA
360 370 380 390 400
VYVYINQQGK WSNVKPIRLN GTKDSMFGIS VKNIGDINQD GYPDIAVGAP
410 420 430 440 450
YDDLGKVFIY HGSPTGIITK PTQVLEGTSP YFGYSIAGNM DLDRNSYPDL
460 470 480 490 500
AVGSLSDSVT IFRSRPVINI LKTITVTPNR IDLRQKSMCG SPSGICLKVK
510 520 530 540 550
ACFEYTAKPS GYNPPISILG ILEAEKERRK SGLSSRVQFR NQGSEPKYTQ
560 570 580 590 600
ELTLNRQKQR ACMEETLWLQ ENIRDKLRPI PITASVEIQE PSSRRRVNSL
610 620 630 640 650
PEVLPILNSN EAKTVQTDVH FLKEGCGDDN VCNSNLKLEY KFGTREGNQD
660 670 680 690 700
KFSYLPIQKG IPELVLKDQK DIALEITVTN SPSDPRNPRK DGDDAHEAKL
710 720 730 740 750
IATFPDTLTY SAYRELRAFP EKQLSCVANQ NGSQADCELG NPFKRNSSVT
760 770 780 790 800
FYLILSTTEV TFDTTDLDIN LKLETTSNQD NLAPITAKAK VVIELLLSVS
810 820 830 840 850
GVAKPSQVYF GGTVVGEQAM KSEDEVGSLI EYEFRVINLG KPLKNLGTAT
860 870 880 890 900
LNIQWPKEIS NGKWLLYLMK VESKGLEQIV CEPHNEINYL KLKESHNSRK
910 920 930 940 950
KRELPEKQID DSRKFSLFPE RKYQTLNCSV NVRCVNIRCP LRGLDSKASL
960 970 980 990 1000
VLRSRLWNST FLEEYSKLNY LDILLRASID VTAAAQNIKL PHAGTQVRVT
1010 1020 1030 1040 1050
VFPSKTVAQY SGVAWWIILL AVLAGILMLA LLVFLLWKCG FFKRSRYDDS
1060 1070 1080 1090
IPRYHAVRIR KEEREIKDEK HMDNLEKKQW ITKWNENESY S
Length:1,091
Mass (Da):122,159
Last modified:July 27, 2011 - v3
Checksum:iF33B055C2E8BAFAD
GO
Isoform Alpha-6X1A (identifier: Q61739-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1045-1091: SRYDDSIPRY...TKWNENESYS → NKKDHYDATYHKAEIHTQPSDKERLTSDA

Show »
Length:1,073
Mass (Da):119,606
Checksum:iC128E9EE14170218
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti592 – 5921S → T in CAA49527. (PubMed:8081870)Curated
Sequence conflicti781 – 7811N → K in CAA49527. (PubMed:8081870)Curated
Sequence conflicti799 – 7991V → L in CAA49527. (PubMed:8081870)Curated
Sequence conflicti946 – 9461S → T in CAA49527. (PubMed:8081870)Curated
Sequence conflicti953 – 9531R → C in CAA49527. (PubMed:8081870)Curated
Sequence conflicti975 – 9751L → V in CAA49527. (PubMed:8081870)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1045 – 109147SRYDD…NESYS → NKKDHYDATYHKAEIHTQPS DKERLTSDA in isoform Alpha-6X1A. 1 PublicationVSP_002726Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69902 mRNA. Translation: CAA49527.1.
AL928963 Genomic DNA. Translation: CAM26370.1.
CCDSiCCDS16118.1. [Q61739-2]
CCDS71074.1. [Q61739-1]
PIRiA40463.
RefSeqiNP_001264899.1. NM_001277970.1. [Q61739-1]
XP_006498868.1. XM_006498805.1. [Q61739-1]
UniGeneiMm.225096.

Genome annotation databases

EnsembliENSMUST00000028522; ENSMUSP00000028522; ENSMUSG00000027111. [Q61739-2]
ENSMUST00000112101; ENSMUSP00000107729; ENSMUSG00000027111. [Q61739-1]
GeneIDi16403.
KEGGimmu:16403.
UCSCiuc008kbd.1. mouse. [Q61739-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69902 mRNA. Translation: CAA49527.1 .
AL928963 Genomic DNA. Translation: CAM26370.1 .
CCDSi CCDS16118.1. [Q61739-2 ]
CCDS71074.1. [Q61739-1 ]
PIRi A40463.
RefSeqi NP_001264899.1. NM_001277970.1. [Q61739-1 ]
XP_006498868.1. XM_006498805.1. [Q61739-1 ]
UniGenei Mm.225096.

3D structure databases

ProteinModelPortali Q61739.
SMRi Q61739. Positions 24-841.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q61739. 1 interaction.
MINTi MINT-225133.
STRINGi 10090.ENSMUSP00000040155.

PTM databases

PhosphoSitei Q61739.

Proteomic databases

MaxQBi Q61739.
PaxDbi Q61739.
PRIDEi Q61739.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000028522 ; ENSMUSP00000028522 ; ENSMUSG00000027111 . [Q61739-2 ]
ENSMUST00000112101 ; ENSMUSP00000107729 ; ENSMUSG00000027111 . [Q61739-1 ]
GeneIDi 16403.
KEGGi mmu:16403.
UCSCi uc008kbd.1. mouse. [Q61739-1 ]

Organism-specific databases

CTDi 3655.
MGIi MGI:96605. Itga6.

Phylogenomic databases

eggNOGi NOG26407.
GeneTreei ENSGT00760000118782.
HOGENOMi HOG000015786.
HOVERGENi HBG108011.
InParanoidi Q61739.
KOi K06485.
OMAi VYINQQG.
OrthoDBi EOG7K3TK7.
TreeFami TF105391.

Enzyme and pathway databases

Reactomei REACT_199046. Assembly of collagen fibrils and other multimeric structures.
REACT_202342. Laminin interactions.
REACT_211508. Basigin interactions.
REACT_216309. Integrin cell surface interactions.
REACT_262098. Type I hemidesmosome assembly.

Miscellaneous databases

PROi Q61739.
SOURCEi Search...

Gene expression databases

Bgeei Q61739.
CleanExi MM_ITGA6.
ExpressionAtlasi Q61739. baseline and differential.
Genevestigatori Q61739.

Family and domain databases

InterProi IPR013519. Int_alpha_beta-p.
IPR000413. Integrin_alpha.
IPR013649. Integrin_alpha-2.
IPR018184. Integrin_alpha_C_CS.
[Graphical view ]
Pfami PF08441. Integrin_alpha2. 1 hit.
[Graphical view ]
PRINTSi PR01185. INTEGRINA.
SMARTi SM00191. Int_alpha. 5 hits.
[Graphical view ]
PROSITEi PS51470. FG_GAP. 7 hits.
PS00242. INTEGRIN_ALPHA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Variants of the alpha 6 beta 1 laminin receptor in early murine development: distribution, molecular cloning and chromosomal localization of the mouse integrin alpha 6 subunit."
    Hierck B.P., Thorsteinsdottir S., Niessen C.M., Freund E., van Iperen L., Feyen A., Hogervorst F., Poelmann R.E., Mummery C.L., Sonnenberg A.
    Cell Adhes. Commun. 1:33-53(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS ALPHA-6X1A AND ALPHA-6X1B).
    Strain: BALB/c.
    Tissue: Mammary gland.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
    Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
    Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-284; ASN-927 AND ASN-958.

Entry informationi

Entry nameiITA6_MOUSE
AccessioniPrimary (citable) accession number: Q61739
Secondary accession number(s): A2AU04
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 27, 2011
Last modified: November 26, 2014
This is version 134 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3