Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Integrin alpha-7

Gene

Itga7

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Integrin alpha-7/beta-1 is the primary laminin receptor on skeletal myoblasts and adult myofibers. During myogenic differentiation, it may induce changes in the shape and mobility of myoblasts, and facilitate their localization at laminin-rich sites of secondary fiber formation. Involved in the maintenance of the myofibers cytoarchitecture as well as for their anchorage, viability and functional integrity. Mice carrying a ITGA7 null allele are viable and fertile, but show progressive muscular dystrophy starting soon after birth, but with a distinct variability in different muscle types. Required to promote contractile phenotype acquisition in differentiated airway smooth muscle (ASM) cells. Acts as Schwann cell receptor for laminin-2. Acts as a receptor of COMP and mediates its effect on vascular smooth muscle cells (VSMCs) maturation (By similarity).By similarity1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi372 – 380Sequence analysis9
Calcium bindingi434 – 442Sequence analysis9
Calcium bindingi492 – 500Sequence analysis9

GO - Molecular functioni

GO - Biological processi

  • blood vessel morphogenesis Source: MGI
  • cell adhesion Source: MGI
  • cell migration Source: MGI
  • heterotypic cell-cell adhesion Source: MGI
  • integrin-mediated signaling pathway Source: UniProtKB-KW
  • regulation of cell shape Source: UniProtKB-KW

Keywordsi

Molecular functionIntegrin, Receptor
Biological processCell adhesion, Cell shape
LigandCalcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Integrin alpha-7
Cleaved into the following 2 chains:
Gene namesi
Name:Itga7
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:102700. Itga7.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini34 – 1076ExtracellularSequence analysisAdd BLAST1043
Transmembranei1077 – 1102HelicalSequence analysisAdd BLAST26
Topological domaini1103 – 1179CytoplasmicSequence analysisAdd BLAST77

GO - Cellular componenti

  • cytoplasm Source: MGI
  • integrin alpha7-beta1 complex Source: MGI
  • muscle tendon junction Source: MGI
  • neuromuscular junction Source: MGI
  • sarcolemma Source: MGI

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 331 PublicationAdd BLAST33
ChainiPRO_000001627034 – 1179Integrin alpha-7Add BLAST1146
ChainiPRO_000001627134 – 955Integrin alpha-7 heavy chainSequence analysisAdd BLAST922
ChainiPRO_0000016272959 – 1179Integrin alpha-7 light chainSequence analysisAdd BLAST221

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi86N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi94 ↔ 103By similarity
Disulfide bondi140 ↔ 163By similarity
Disulfide bondi184 ↔ 197By similarity
Disulfide bondi539 ↔ 546By similarity
Disulfide bondi552 ↔ 615By similarity
Disulfide bondi681 ↔ 687By similarity
Disulfide bondi781 ↔ 792By similarity
Glycosylationi784N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi939 ↔ 993Interchain (between heavy and light chains)By similarity
Glycosylationi988N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi999 ↔ 1004By similarity
Glycosylationi1023N-linked (GlcNAc...)1 Publication1
Glycosylationi1043N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

ADP-ribosylated on at least two sites of the extracellular domain in skeletal myotubes (in vitro).
No proteolytic cleavage to produce the 70 kDa form is seen due to the presence of a Gly instead of an arginine residue at position 647.

Keywords - PTMi

ADP-ribosylation, Cleavage on pair of basic residues, Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ61738.
PeptideAtlasiQ61738.
PRIDEiQ61738.

PTM databases

iPTMnetiQ61738.
PhosphoSitePlusiQ61738.

Expressioni

Tissue specificityi

Isoforms containing segment X2 are found in adult heart, lung and skeletal muscle. Isoforms containing segment X1 are expressed in adult heart, lung and in proliferating skeletal myoblasts but not in adult skeletal muscle. Isoforms containing segment a are exclusively found in skeletal muscle. Isoforms containing segment B are widely expressed. In muscle fibers isoforms containing segment A and B are expressed at myotendinous and neuromuscular junctions; isoforms containing segment C are expressed at neuromuscular junctions and at extrasynaptic sites.1 Publication

Developmental stagei

Isoforms are developmentally regulated during the formation of skeletal muscle. Undifferentiated (replicating) myoblasts express isoforms containing segment B only, whereas differentiated myoblasts express isoforms containing segments A or B.

Gene expression databases

CleanExiMM_ITGA7.

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of a heavy and a light chain linked by a disulfide bond. Alpha-7 associates with beta-1. Interacts with COMP. Interacts (via C-terminus intracellular tail region) with CIB1; the interaction is stabilized/increased in a calcium- and magnesium-dependent manner (By similarity).By similarity

Binary interactionsi

Show more details

Protein-protein interaction databases

IntActiQ61738. 5 interactors.
MINTiMINT-4098899.
STRINGi10090.ENSMUSP00000096712.

Structurei

3D structure databases

ProteinModelPortaliQ61738.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati38 – 103FG-GAP 1PROSITE-ProRule annotationAdd BLAST66
Repeati110 – 175FG-GAP 2PROSITE-ProRule annotationAdd BLAST66
Repeati185 – 238FG-GAP 3PROSITE-ProRule annotationAdd BLAST54
Repeati292 – 349FG-GAP 4PROSITE-ProRule annotationAdd BLAST58
Repeati350 – 411FG-GAP 5PROSITE-ProRule annotationAdd BLAST62
Repeati412 – 467FG-GAP 6PROSITE-ProRule annotationAdd BLAST56
Repeati471 – 530FG-GAP 7PROSITE-ProRule annotationAdd BLAST60
Repeati1155 – 115814
Repeati1163 – 116624
Repeati1171 – 117434

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1155 – 11743 X 4 AA repeats of D-X-H-PAdd BLAST20

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi1105 – 1109GFFKR motif5

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi953 – 958Poly-Arg6

Sequence similaritiesi

Belongs to the integrin alpha chain family.Curated

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IPBB. Eukaryota.
ENOG410XVGZ. LUCA.
HOGENOMiHOG000015786.
HOVERGENiHBG108011.
InParanoidiQ61738.
PhylomeDBiQ61738.

Family and domain databases

InterProiView protein in InterPro
IPR013517. FG-GAP.
IPR013519. Int_alpha_beta-p.
IPR000413. Integrin_alpha.
IPR013649. Integrin_alpha-2.
IPR018184. Integrin_alpha_C_CS.
IPR032695. Integrin_dom.
PfamiView protein in Pfam
PF01839. FG-GAP. 2 hits.
PF08441. Integrin_alpha2. 1 hit.
PRINTSiPR01185. INTEGRINA.
SMARTiView protein in SMART
SM00191. Int_alpha. 5 hits.
SUPFAMiSSF69179. SSF69179. 3 hits.
PROSITEiView protein in PROSITE
PS51470. FG_GAP. 7 hits.
PS00242. INTEGRIN_ALPHA. 1 hit.

Sequences (6)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 6 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist. There is a combination of at least four alternatively spliced domains, two extracellular (X1 and X2) and two cytoplasmic (A and B). A third potential alternatively spliced cytoplasmic domain (C) doesn't appear to be expressed. So far detected are isoform alpha-7X1A, isoform alpha-7X1B and isoform alpha-7X2B. Experimental confirmation may be lacking for some isoforms.
Isoform Alpha-7X1X2B (identifier: Q61738-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MARIPRCDFL RPPGIYYLIT SLLAGLFLPP AIAFNLDVMG AIRKEGEPGS
60 70 80 90 100
LFGFSVALHR QLQPRPQSWL LVGAPQALAL PGQQANRTGG LFACPLSLEE
110 120 130 140 150
TDCYRVDIDR GANVQKESKE NQWLGVSVRS QGAGGKIVTC AHRYESRQRV
160 170 180 190 200
DQALETRDVI GRCFVLSQDL AIRDELDGGE WKFCEGRPQG HEQFGFCQQG
210 220 230 240 250
TAATFSPDSH YLVFGAPGTY NWKGTARVEL CAQGSPDLAH LDDGPYEAGG
260 270 280 290 300
EKEQDPRLIP VPANSYLGLL FVTNIDSSDP DQLVYKTLDP ADRLTGPAGD
310 320 330 340 350
LTLNSYLGFS IDSGKGLMRS EELSFVAGAP RANHKGAVVI LRKDSATRLI
360 370 380 390 400
PEVVLSGERL TSGFGYSLAV TDLNNDGWAD LIVGAPYFFE RQEELGGAVY
410 420 430 440 450
VYMNQGGHWA DISPLRICGS PDSMFGISLA VLGDLNQDGF PDIAVGAPFD
460 470 480 490 500
GDGKVFIYHG SSLGVVVKPS QVLEGEAVGI KSFGYSLSGG LDVDGNHYPD
510 520 530 540 550
LLVGSLADTA ALFRARPVLH VSQEIFIDPR AIDLEQPNCA DGRLVCVDIK
560 570 580 590 600
ICFSYVAVPS SYSPSVALDY MLDGDTDRRL RGQVPRVTFL SRGLDDLRHQ
610 620 630 640 650
SSGTVWLKHQ HDRVCGDTVF QLQENVKDKL RAIVVTLSYG LRTPPLGRQA
660 670 680 690 700
PGQELPTVAP ILNAHQPSTQ RTEIHFLKQG CGQDKICQSN LQLERYQFCS
710 720 730 740 750
RISDTEFQAL PMDLDGRTAL FALSGQPFIG LELTVTNLPS DPSRPQADGD
760 770 780 790 800
DAHEAQLLVT LPASLRYSGV RALDSVEKPL CLSNDSASHV ECELGNPMKR
810 820 830 840 850
GAQVTFYLIL STSGITIETT ELEVKLLLAT ISEQELDPVS VRAHVFIELP
860 870 880 890 900
LSISGVATPQ QLFFSGEVKG ESAMRSEREL GRKVKYEVTV SNQGQSLNTL
910 920 930 940 950
GSANLNIMWP HEIANGKWLL YPMRVELEGG QGPGKRGICS PRPNILQLDV
960 970 980 990 1000
DSRDRRRREL GQPEPQEPPE KVEPSTSWWP VSSAEKRNMT LDCPRTAKCV
1010 1020 1030 1040 1050
VFSCPLYSFD RAAVLHVWGR LWNSTFLEEY MAVKSLEVIV RANITVKSSI
1060 1070 1080 1090 1100
KNLLLRDAST VIPVMVYLDP MAVVVEGVPW WVILLGVLAG LLVLALLVLL
1110 1120 1130 1140 1150
LWKLGFFKRA KHPEATVPQY HAVKIPREDR QQFKEEKTGT IQRSNWGNSQ
1160 1170
WEGSDAHPIL AADWHPELGP DGHPVPATA
Length:1,179
Mass (Da):129,329
Last modified:October 5, 2010 - v3
Checksum:i3C26D5BABF2E463D
GO
Isoform Alpha-7X1A (identifier: Q61738-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     268-307: Missing.
     1104-1179: LGFFKRAKHP...PDGHPVPATA → CGFFRRNSPS...PRPPCPSTTQ

Show »
Length:1,120
Mass (Da):122,492
Checksum:iC47BD71A00A96C14
GO
Isoform Alpha-7X1B (identifier: Q61738-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     268-307: Missing.

Show »
Length:1,139
Mass (Da):125,037
Checksum:i318EE0ED64709E7B
GO
Isoform Alpha-7X2A (identifier: Q61738-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     224-267: Missing.
     1104-1179: LGFFKRAKHP...PDGHPVPATA → CGFFRRNSPS...PRPPCPSTTQ

Show »
Length:1,116
Mass (Da):122,151
Checksum:iF9ED8397C6373324
GO
Isoform Alpha-7X2B (identifier: Q61738-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     224-267: Missing.

Show »
Length:1,135
Mass (Da):124,696
Checksum:iB4C1D6D65C7E3676
GO
Isoform Alpha-7X1X2A (identifier: Q61738-6) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1104-1179: LGFFKRAKHP...PDGHPVPATA → CGFFRRNSPS...PRPPCPSTTQ

Show »
Length:1,160
Mass (Da):126,784
Checksum:iCAF7035497AAA400
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti133A → P in CAA73023 (Ref. 2) Curated1
Sequence conflicti133A → P in CAA73024 (Ref. 2) Curated1
Sequence conflicti347T → Q in AAA16599 (PubMed:8253814).Curated1
Sequence conflicti347T → S in CAA73023 (Ref. 2) Curated1
Sequence conflicti347T → S in CAA73024 (Ref. 2) Curated1
Sequence conflicti647G → R in AAA16600 (PubMed:8253814).Curated1
Sequence conflicti879 – 880EL → DV in CAA73023 (Ref. 2) Curated2
Sequence conflicti879 – 880EL → DV in CAA73024 (Ref. 2) Curated2
Sequence conflicti882R → S in CAA73023 (Ref. 2) Curated1
Sequence conflicti882R → S in CAA73024 (Ref. 2) Curated1
Sequence conflicti904N → F in CAA73023 (Ref. 2) Curated1
Sequence conflicti904N → F in CAA73024 (Ref. 2) Curated1
Sequence conflicti989M → V in CAA73023 (Ref. 2) Curated1
Sequence conflicti989M → V in CAA73024 (Ref. 2) Curated1
Sequence conflicti994 – 995PR → AQG in CAA73023 (Ref. 2) Curated2
Sequence conflicti994 – 995PR → AQG in CAA73024 (Ref. 2) Curated2
Sequence conflicti1031 – 1034MAVK → LLIN in AAA16598 (PubMed:8253814).Curated4
Sequence conflicti1086G → A in CAA73023 (Ref. 2) Curated1
Sequence conflicti1086G → A in CAA73024 (Ref. 2) Curated1
Isoform Alpha-7X2A (identifier: Q61738-4)
Sequence conflicti1042G → A in L16544 (PubMed:8360188).Curated1
Sequence conflicti1115 – 1116TQ → T in AAA16598 (PubMed:8253814).Curated2
Isoform Alpha-7X1A (identifier: Q61738-2)
Sequence conflicti1046G → A in L16544 (PubMed:8360188).Curated1
Sequence conflicti1119 – 1120TQ → T in AAA16598 (PubMed:8253814).Curated2
Isoform Alpha-7X1X2A (identifier: Q61738-6)
Sequence conflicti1086G → A in L16544 (PubMed:8360188).Curated1
Sequence conflicti1159 – 1160TQ → T in AAA16598 (PubMed:8253814).Curated2

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_002731224 – 267Missing in isoform Alpha-7X2A and isoform Alpha-7X2B. 1 PublicationAdd BLAST44
Alternative sequenceiVSP_002732268 – 307Missing in isoform Alpha-7X1A and isoform Alpha-7X1B. CuratedAdd BLAST40
Alternative sequenceiVSP_0027331104 – 1179LGFFK…VPATA → CGFFRRNSPSSSFPTNYHRA HLAVQPSAMEAGGPGTVGWD SSSGRSTPRPPCPSTTQ in isoform Alpha-7X1A, isoform Alpha-7X2A and isoform Alpha-7X1X2A. CuratedAdd BLAST76

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L23423 mRNA. Translation: AAA16600.1.
Y12380
, Y12383, Y12384, Y12385, Y12386, Y12387, Y12388, Y12389, Y12390, Y12382 Genomic DNA. Translation: CAA73023.1.
L23422 Genomic DNA. Translation: AAA16599.1.
Y12380
, Y12383, Y12384, Y12385, Y12386, Y12387, Y12388, Y12389, Y12390, Y12381, Y12382 Genomic DNA. Translation: CAA73024.1.
U60419 Genomic DNA. Translation: AAC52772.1.
L23421 mRNA. Translation: AAA16598.1.
L16544 mRNA. No translation available.
PIRiI61186.
I61187.
UniGeneiMm.179747.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L23423 mRNA. Translation: AAA16600.1.
Y12380
, Y12383, Y12384, Y12385, Y12386, Y12387, Y12388, Y12389, Y12390, Y12382 Genomic DNA. Translation: CAA73023.1.
L23422 Genomic DNA. Translation: AAA16599.1.
Y12380
, Y12383, Y12384, Y12385, Y12386, Y12387, Y12388, Y12389, Y12390, Y12381, Y12382 Genomic DNA. Translation: CAA73024.1.
U60419 Genomic DNA. Translation: AAC52772.1.
L23421 mRNA. Translation: AAA16598.1.
L16544 mRNA. No translation available.
PIRiI61186.
I61187.
UniGeneiMm.179747.

3D structure databases

ProteinModelPortaliQ61738.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ61738. 5 interactors.
MINTiMINT-4098899.
STRINGi10090.ENSMUSP00000096712.

PTM databases

iPTMnetiQ61738.
PhosphoSitePlusiQ61738.

Proteomic databases

PaxDbiQ61738.
PeptideAtlasiQ61738.
PRIDEiQ61738.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:102700. Itga7.

Phylogenomic databases

eggNOGiENOG410IPBB. Eukaryota.
ENOG410XVGZ. LUCA.
HOGENOMiHOG000015786.
HOVERGENiHBG108011.
InParanoidiQ61738.
PhylomeDBiQ61738.

Miscellaneous databases

PROiQ61738.
SOURCEiSearch...

Gene expression databases

CleanExiMM_ITGA7.

Family and domain databases

InterProiView protein in InterPro
IPR013517. FG-GAP.
IPR013519. Int_alpha_beta-p.
IPR000413. Integrin_alpha.
IPR013649. Integrin_alpha-2.
IPR018184. Integrin_alpha_C_CS.
IPR032695. Integrin_dom.
PfamiView protein in Pfam
PF01839. FG-GAP. 2 hits.
PF08441. Integrin_alpha2. 1 hit.
PRINTSiPR01185. INTEGRINA.
SMARTiView protein in SMART
SM00191. Int_alpha. 5 hits.
SUPFAMiSSF69179. SSF69179. 3 hits.
PROSITEiView protein in PROSITE
PS51470. FG_GAP. 7 hits.
PS00242. INTEGRIN_ALPHA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiITA7_MOUSE
AccessioniPrimary (citable) accession number: Q61738
Secondary accession number(s): O88731
, O88732, P70350, Q61737, Q61741
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: October 5, 2010
Last modified: February 15, 2017
This is version 144 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.