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Q61738 (ITA7_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 130. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Integrin alpha-7
Gene names
Name:Itga7
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1179 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Integrin alpha-7/beta-1 is the primary laminin receptor on skeletal myoblasts and adult myofibers. During myogenic differentiation, it may induce changes in the shape and mobility of myoblasts, and facilitate their localization at laminin-rich sites of secondary fiber formation. Involved in the maintenance of the myofibers cytoarchitecture as well as for their anchorage, viability and functional integrity. Mice carrying a ITGA7 null allele are viable and fertile, but show progressive muscular dystrophy starting soon after birth, but with a distinct variability in different muscle types. Required to promote contractile phenotype acquisition in differentiated airway smooth muscle (ASM) cells. Acts as Schwann cell receptor for laminin-2. Acts as a receptor of COMP and mediates its effect on vascular smooth muscle cells (VSMCs) maturation By similarity. Ref.7

Subunit structure

Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of a heavy and a light chain linked by a disulfide bond. Alpha-7 associates with beta-1. Interacts with COMP. Interacts (via C-terminus intracellular tail region) with CIB1; the interaction is stabilized/increased in a calcium- and magnesium-dependent manner By similarity.

Subcellular location

Membrane; Single-pass type I membrane protein.

Tissue specificity

Isoforms containing segment X2 are found in adult heart, lung and skeletal muscle. Isoforms containing segment X1 are expressed in adult heart, lung and in proliferating skeletal myoblasts but not in adult skeletal muscle. Isoforms containing segment a are exclusively found in skeletal muscle. Isoforms containing segment B are widely expressed. In muscle fibers isoforms containing segment A and B are expressed at myotendinous and neuromuscular junctions; isoforms containing segment C are expressed at neuromuscular junctions and at extrasynaptic sites. Ref.6

Developmental stage

Isoforms are developmentally regulated during the formation of skeletal muscle. Undifferentiated (replicating) myoblasts express isoforms containing segment B only, whereas differentiated myoblasts express isoforms containing segments A or B.

Post-translational modification

ADP-ribosylated on at least two sites of the extracellular domain in skeletal myotubes (in vitro).

No proteolytic cleavage to produce the 70 kDa form is seen due to the presence of a Gly instead of an arginine residue at position 647.

Sequence similarities

Belongs to the integrin alpha chain family.

Contains 7 FG-GAP repeats.

Binary interactions

Alternative products

This entry describes 6 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist. There is a combination of at least four alternatively spliced domains, two extracellular (X1 and X2) and two cytoplasmic (A and B). A third potential alternatively spliced cytoplasmic domain (C) doesn't appear to be expressed. So far detected are isoform alpha-7X1A, isoform alpha-7X1B and isoform alpha-7X2B. Experimental confirmation may be lacking for some isoforms.
Isoform Alpha-7X1X2B (identifier: Q61738-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Alpha-7X1A (identifier: Q61738-2)

The sequence of this isoform differs from the canonical sequence as follows:
     268-307: Missing.
     1104-1179: LGFFKRAKHP...PDGHPVPATA → CGFFRRNSPS...PRPPCPSTTQ
Isoform Alpha-7X1B (identifier: Q61738-3)

The sequence of this isoform differs from the canonical sequence as follows:
     268-307: Missing.
Isoform Alpha-7X2A (identifier: Q61738-4)

The sequence of this isoform differs from the canonical sequence as follows:
     224-267: Missing.
     1104-1179: LGFFKRAKHP...PDGHPVPATA → CGFFRRNSPS...PRPPCPSTTQ
Isoform Alpha-7X2B (identifier: Q61738-5)

The sequence of this isoform differs from the canonical sequence as follows:
     224-267: Missing.
Isoform Alpha-7X1X2A (identifier: Q61738-6)

The sequence of this isoform differs from the canonical sequence as follows:
     1104-1179: LGFFKRAKHP...PDGHPVPATA → CGFFRRNSPS...PRPPCPSTTQ

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3333 Ref.4
Chain34 – 11791146Integrin alpha-7
PRO_0000016270
Chain34 – 955922Integrin alpha-7 heavy chain Potential
PRO_0000016271
Chain959 – 1179221Integrin alpha-7 light chain Potential
PRO_0000016272

Regions

Topological domain34 – 10761043Extracellular Potential
Transmembrane1077 – 110226Helical; Potential
Topological domain1103 – 117977Cytoplasmic Potential
Repeat38 – 10366FG-GAP 1
Repeat110 – 17566FG-GAP 2
Repeat185 – 23854FG-GAP 3
Repeat292 – 35059FG-GAP 4
Repeat351 – 41161FG-GAP 5
Repeat412 – 46756FG-GAP 6
Repeat471 – 53060FG-GAP 7
Repeat1155 – 115841
Repeat1163 – 116642
Repeat1171 – 117443
Calcium binding372 – 3809 Potential
Calcium binding434 – 4429 Potential
Calcium binding492 – 5009 Potential
Region1155 – 1174203 X 4 AA repeats of D-X-H-P
Motif1105 – 11095GFFKR motif
Compositional bias953 – 9586Poly-Arg

Amino acid modifications

Glycosylation861N-linked (GlcNAc...) Potential
Glycosylation7841N-linked (GlcNAc...) Potential
Glycosylation9881N-linked (GlcNAc...) Potential
Glycosylation10231N-linked (GlcNAc...) Ref.10
Glycosylation10431N-linked (GlcNAc...) Potential
Disulfide bond94 ↔ 103 By similarity
Disulfide bond140 ↔ 163 By similarity
Disulfide bond184 ↔ 197 By similarity
Disulfide bond539 ↔ 546 By similarity
Disulfide bond552 ↔ 615 By similarity
Disulfide bond681 ↔ 687 By similarity
Disulfide bond781 ↔ 792 By similarity
Disulfide bond939 ↔ 993Interchain (between heavy and light chains) By similarity
Disulfide bond999 ↔ 1004 By similarity

Natural variations

Alternative sequence224 – 26744Missing in isoform Alpha-7X2A and isoform Alpha-7X2B.
VSP_002731
Alternative sequence268 – 30740Missing in isoform Alpha-7X1A and isoform Alpha-7X1B.
VSP_002732
Alternative sequence1104 – 117976LGFFK…VPATA → CGFFRRNSPSSSFPTNYHRA HLAVQPSAMEAGGPGTVGWD SSSGRSTPRPPCPSTTQ in isoform Alpha-7X1A, isoform Alpha-7X2A and isoform Alpha-7X1X2A.
VSP_002733

Experimental info

Sequence conflict1331A → P in CAA73023. Ref.2
Sequence conflict1331A → P in CAA73024. Ref.2
Sequence conflict3471T → Q in AAA16599. Ref.1
Sequence conflict3471T → S in CAA73023. Ref.2
Sequence conflict3471T → S in CAA73024. Ref.2
Sequence conflict6471G → R in AAA16600. Ref.1
Sequence conflict879 – 8802EL → DV in CAA73023. Ref.2
Sequence conflict879 – 8802EL → DV in CAA73024. Ref.2
Sequence conflict8821R → S in CAA73023. Ref.2
Sequence conflict8821R → S in CAA73024. Ref.2
Sequence conflict9041N → F in CAA73023. Ref.2
Sequence conflict9041N → F in CAA73024. Ref.2
Sequence conflict9891M → V in CAA73023. Ref.2
Sequence conflict9891M → V in CAA73024. Ref.2
Sequence conflict994 – 9952PR → AQG in CAA73023. Ref.2
Sequence conflict994 – 9952PR → AQG in CAA73024. Ref.2
Sequence conflict1031 – 10344MAVK → LLIN in AAA16598. Ref.1
Sequence conflict10861G → A in CAA73023. Ref.2
Sequence conflict10861G → A in CAA73024. Ref.2
Isoform Alpha-7X1A:
Sequence conflict10461G → A in L16544. Ref.5
Sequence conflict1119 – 11202TQ → T in AAA16598. Ref.1
Isoform Alpha-7X2A:
Sequence conflict10421G → A in L16544. Ref.5
Sequence conflict1115 – 11162TQ → T in AAA16598. Ref.1
Isoform Alpha-7X1X2A:
Sequence conflict10861G → A in L16544. Ref.5
Sequence conflict1159 – 11602TQ → T in AAA16598. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform Alpha-7X1X2B [UniParc].

Last modified October 5, 2010. Version 3.
Checksum: 3C26D5BABF2E463D

FASTA1,179129,329
        10         20         30         40         50         60 
MARIPRCDFL RPPGIYYLIT SLLAGLFLPP AIAFNLDVMG AIRKEGEPGS LFGFSVALHR 

        70         80         90        100        110        120 
QLQPRPQSWL LVGAPQALAL PGQQANRTGG LFACPLSLEE TDCYRVDIDR GANVQKESKE 

       130        140        150        160        170        180 
NQWLGVSVRS QGAGGKIVTC AHRYESRQRV DQALETRDVI GRCFVLSQDL AIRDELDGGE 

       190        200        210        220        230        240 
WKFCEGRPQG HEQFGFCQQG TAATFSPDSH YLVFGAPGTY NWKGTARVEL CAQGSPDLAH 

       250        260        270        280        290        300 
LDDGPYEAGG EKEQDPRLIP VPANSYLGLL FVTNIDSSDP DQLVYKTLDP ADRLTGPAGD 

       310        320        330        340        350        360 
LTLNSYLGFS IDSGKGLMRS EELSFVAGAP RANHKGAVVI LRKDSATRLI PEVVLSGERL 

       370        380        390        400        410        420 
TSGFGYSLAV TDLNNDGWAD LIVGAPYFFE RQEELGGAVY VYMNQGGHWA DISPLRICGS 

       430        440        450        460        470        480 
PDSMFGISLA VLGDLNQDGF PDIAVGAPFD GDGKVFIYHG SSLGVVVKPS QVLEGEAVGI 

       490        500        510        520        530        540 
KSFGYSLSGG LDVDGNHYPD LLVGSLADTA ALFRARPVLH VSQEIFIDPR AIDLEQPNCA 

       550        560        570        580        590        600 
DGRLVCVDIK ICFSYVAVPS SYSPSVALDY MLDGDTDRRL RGQVPRVTFL SRGLDDLRHQ 

       610        620        630        640        650        660 
SSGTVWLKHQ HDRVCGDTVF QLQENVKDKL RAIVVTLSYG LRTPPLGRQA PGQELPTVAP 

       670        680        690        700        710        720 
ILNAHQPSTQ RTEIHFLKQG CGQDKICQSN LQLERYQFCS RISDTEFQAL PMDLDGRTAL 

       730        740        750        760        770        780 
FALSGQPFIG LELTVTNLPS DPSRPQADGD DAHEAQLLVT LPASLRYSGV RALDSVEKPL 

       790        800        810        820        830        840 
CLSNDSASHV ECELGNPMKR GAQVTFYLIL STSGITIETT ELEVKLLLAT ISEQELDPVS 

       850        860        870        880        890        900 
VRAHVFIELP LSISGVATPQ QLFFSGEVKG ESAMRSEREL GRKVKYEVTV SNQGQSLNTL 

       910        920        930        940        950        960 
GSANLNIMWP HEIANGKWLL YPMRVELEGG QGPGKRGICS PRPNILQLDV DSRDRRRREL 

       970        980        990       1000       1010       1020 
GQPEPQEPPE KVEPSTSWWP VSSAEKRNMT LDCPRTAKCV VFSCPLYSFD RAAVLHVWGR 

      1030       1040       1050       1060       1070       1080 
LWNSTFLEEY MAVKSLEVIV RANITVKSSI KNLLLRDAST VIPVMVYLDP MAVVVEGVPW 

      1090       1100       1110       1120       1130       1140 
WVILLGVLAG LLVLALLVLL LWKLGFFKRA KHPEATVPQY HAVKIPREDR QQFKEEKTGT 

      1150       1160       1170 
IQRSNWGNSQ WEGSDAHPIL AADWHPELGP DGHPVPATA 

« Hide

Isoform Alpha-7X1A [UniParc].

Checksum: C47BD71A00A96C14
Show »

FASTA1,120122,492
Isoform Alpha-7X1B [UniParc].

Checksum: 318EE0ED64709E7B
Show »

FASTA1,139125,037
Isoform Alpha-7X2A [UniParc].

Checksum: F9ED8397C6373324
Show »

FASTA1,116122,151
Isoform Alpha-7X2B [UniParc].

Checksum: B4C1D6D65C7E3676
Show »

FASTA1,135124,696
Isoform Alpha-7X1X2A [UniParc].

Checksum: CAF7035497AAA400
Show »

FASTA1,160126,784

References

« Hide 'large scale' references
[1]"Alternative extracellular and cytoplasmic domains of the integrin alpha 7 subunit are differentially expressed during development."
Ziober B.L., Vu M.P., Waleh N., Crawford J., Lin C.-S., Kramer R.H.
J. Biol. Chem. 268:26773-26783(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM ALPHA-7X2B), NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-70 (ISOFORM ALPHA-7X1X2B), NUCLEOTIDE SEQUENCE [MRNA] OF 1031-1179 (ISOFORMS ALPHA-7X1A/ALPHA-7X2A/ALPHA-7X1X2A), ALTERNATIVE SPLICING.
Strain: BALB/c.
Tissue: Heart.
[2]"Genomic organization and chromosomal localization of the mouse integrin alpha7 gene."
Saher G., Echtermeyer F., Beier D.R., Poeschl E., Mayer U.
Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS ALPHA-7X1X2A AND ALPHA-7X1X2B).
Strain: 129/Sv.
[3]"Identification and characterization of the cell type-specific and developmentally regulated alpha7 integrin gene promoter."
Ziober B.L., Kramer R.H.
J. Biol. Chem. 271:22915-22922(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-70.
Strain: C57BL/6 X CBA.
[4]"Laminin-binding integrin alpha 7 beta 1: functional characterization and expression in normal and malignant melanocytes."
Kramer R.H., Vu M.P., Cheng Y.F., Ramos D.M., Timpl R., Waleh N.
Cell Regul. 2:805-817(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 34-58.
Tissue: Melanoma.
[5]"A new isoform of the laminin receptor integrin alpha 7 beta 1 is developmentally regulated in skeletal muscle."
Collo G., Starr L., Quaranta V.
J. Biol. Chem. 268:19019-19024(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1013-1179 (ISOFORMS ALPHA-7X1A/ALPHA-7X2A/ALPHA-7X1X2A).
Strain: C57BL/6 X BALB/c.
[6]"Synaptic integrins in developing, adult, and mutant muscle: selective association of alpha1, alpha7A, and alpha7B integrins with the neuromuscular junction."
Martin P.T., Kaufman S.J., Kramer R.H., Sanes J.R.
Dev. Biol. 174:125-139(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[7]"Absence of integrin alpha 7 causes a novel form of muscular dystrophy."
Mayer U., Saher G., Fassler R., Bornemann A., Echtermeyer F., von der Mark H., Miosge N., Poeschl E., von der Mark K.
Nat. Genet. 17:318-323(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[8]"Processing of ADP-ribosylated integrin alpha 7 in skeletal muscle myotubes."
Zolkiewska A., Moss J.
J. Biol. Chem. 270:9227-9233(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: ADP-RIBOSYLATION.
[9]"Genetically determined proteolytic cleavage modulates alpha7beta1 integrin function."
Liu J., Gurpur P.B., Kaufman S.J.
J. Biol. Chem. 283:35668-35678(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: ABSENCE OF CLEAVAGE BY UROKINASE.
[10]"The mouse C2C12 myoblast cell surface N-linked glycoproteome: identification, glycosite occupancy, and membrane orientation."
Gundry R.L., Raginski K., Tarasova Y., Tchernyshyov I., Bausch-Fluck D., Elliott S.T., Boheler K.R., Van Eyk J.E., Wollscheid B.
Mol. Cell. Proteomics 8:2555-2569(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-1023.
Tissue: Myoblast.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L23423 mRNA. Translation: AAA16600.1.
Y12380 expand/collapse EMBL AC list , Y12383, Y12384, Y12385, Y12386, Y12387, Y12388, Y12389, Y12390, Y12382 Genomic DNA. Translation: CAA73023.1.
L23422 Genomic DNA. Translation: AAA16599.1.
Y12380 expand/collapse EMBL AC list , Y12383, Y12384, Y12385, Y12386, Y12387, Y12388, Y12389, Y12390, Y12381, Y12382 Genomic DNA. Translation: CAA73024.1.
U60419 Genomic DNA. Translation: AAC52772.1.
L23421 mRNA. Translation: AAA16598.1.
L16544 mRNA. No translation available.
PIRI61186.
I61187.
UniGeneMm.179747.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ61738. 5 interactions.
MINTMINT-4098899.

PTM databases

PhosphoSiteQ61738.

Proteomic databases

MaxQBQ61738.
PaxDbQ61738.
PRIDEQ61738.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

MGIMGI:102700. Itga7.

Phylogenomic databases

eggNOGNOG26407.
HOGENOMHOG000015786.
HOVERGENHBG108011.
InParanoidQ61738.
PhylomeDBQ61738.

Gene expression databases

CleanExMM_ITGA7.
GenevestigatorQ61738.

Family and domain databases

InterProIPR013519. Int_alpha_beta-p.
IPR000413. Integrin_alpha.
IPR013649. Integrin_alpha-2.
IPR018184. Integrin_alpha_C_CS.
[Graphical view]
PfamPF08441. Integrin_alpha2. 1 hit.
[Graphical view]
PRINTSPR01185. INTEGRINA.
SMARTSM00191. Int_alpha. 5 hits.
[Graphical view]
PROSITEPS51470. FG_GAP. 7 hits.
PS00242. INTEGRIN_ALPHA. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROQ61738.
SOURCESearch...

Entry information

Entry nameITA7_MOUSE
AccessionPrimary (citable) accession number: Q61738
Secondary accession number(s): O88731 expand/collapse secondary AC list , O88732, P70350, Q61737, Q61741
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: October 5, 2010
Last modified: June 11, 2014
This is version 130 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot