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Protein

Leukocyte surface antigen CD47

Gene

Cd47

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus. Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • cell adhesion Source: UniProtKB-KW
  • cell migration Source: Ensembl
  • opsonization Source: MGI
  • positive regulation of cell-cell adhesion Source: MGI
  • positive regulation of cell proliferation Source: MGI
  • positive regulation of inflammatory response Source: MGI
  • positive regulation of phagocytosis Source: MGI
  • positive regulation of T cell activation Source: MGI
  • response to bacterium Source: MGI
Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Enzyme and pathway databases

ReactomeiR-MMU-202733. Cell surface interactions at the vascular wall.
R-MMU-216083. Integrin cell surface interactions.
R-MMU-391160. Signal regulatory protein (SIRP) family interactions.

Names & Taxonomyi

Protein namesi
Recommended name:
Leukocyte surface antigen CD47
Alternative name(s):
Integrin-associated protein
Short name:
IAP
CD_antigen: CD47
Gene namesi
Name:Cd47
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:96617. Cd47.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini19 – 140122ExtracellularSequence analysisAdd
BLAST
Transmembranei141 – 16121HelicalSequence analysisAdd
BLAST
Topological domaini162 – 17312CytoplasmicSequence analysisAdd
BLAST
Transmembranei174 – 19421HelicalSequence analysisAdd
BLAST
Topological domaini195 – 20612ExtracellularSequence analysisAdd
BLAST
Transmembranei207 – 22721HelicalSequence analysisAdd
BLAST
Topological domaini228 – 23811CytoplasmicSequence analysisAdd
BLAST
Transmembranei239 – 25921HelicalSequence analysisAdd
BLAST
Topological domaini260 – 2667ExtracellularSequence analysis
Transmembranei267 – 28721HelicalSequence analysisAdd
BLAST
Topological domaini288 – 30316CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • extracellular exosome Source: MGI
  • integral component of plasma membrane Source: MGI
  • plasma membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818Sequence analysisAdd
BLAST
Chaini19 – 303285Leukocyte surface antigen CD47PRO_0000042206Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei19 – 191Pyrrolidone carboxylic acidCurated
Disulfide bondi33 ↔ 261PROSITE-ProRule annotation
Glycosylationi34 – 341N-linked (GlcNAc...)Sequence analysis
Disulfide bondi41 ↔ 112PROSITE-ProRule annotation
Glycosylationi61 – 611N-linked (GlcNAc...)Sequence analysis
Glycosylationi73 – 731N-linked (GlcNAc...)Sequence analysis
Glycosylationi80 – 801N-linked (GlcNAc...)Sequence analysis
Modified residuei87 – 871PhosphoserineBy similarity
Modified residuei89 – 891PhosphoserineBy similarity
Glycosylationi109 – 1091N-linked (GlcNAc...)Sequence analysis
Glycosylationi129 – 1291N-linked (GlcNAc...)Sequence analysis
Glycosylationi204 – 2041N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein, Pyrrolidone carboxylic acid

Proteomic databases

EPDiQ61735.
MaxQBiQ61735.
PeptideAtlasiQ61735.
PRIDEiQ61735.

PTM databases

PhosphoSiteiQ61735.
SwissPalmiQ61735.

Expressioni

Gene expression databases

BgeeiQ61735.
ExpressionAtlasiQ61735. baseline and differential.
GenevisibleiQ61735. MM.

Interactioni

Subunit structurei

Monomer. Interacts with fibrinogen, PTPNS1, SIRPG, THBS1, UBQLN1 and UBQLN2 (By similarity).By similarity

Protein-protein interaction databases

BioGridi200835. 2 interactions.
IntActiQ61735. 5 interactions.
MINTiMINT-1711744.

Structurei

3D structure databases

ProteinModelPortaliQ61735.
SMRiQ61735. Positions 19-134.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini19 – 125107Ig-like V-typeAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000007697.
HOGENOMiHOG000013020.
HOVERGENiHBG003808.
InParanoidiQ61735.
KOiK06266.
OMAiVYMKFVA.
OrthoDBiEOG79GT78.
PhylomeDBiQ61735.
TreeFamiTF336026.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
InterProiIPR006704. CD47.
IPR013147. CD47_TM.
IPR013270. CD47_Vset.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
[Graphical view]
PANTHERiPTHR10613. PTHR10613. 1 hit.
PfamiPF04549. CD47. 1 hit.
PF08204. V-set_CD47. 1 hit.
[Graphical view]
PROSITEiPS50835. IG_LIKE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q61735-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MWPLAAALLL GSCCCGSAQL LFSNVNSIEF TSCNETVVIP CIVRNVEAQS
60 70 80 90 100
TEEMFVKWKL NKSYIFIYDG NKNSTTTDQN FTSAKISVSD LINGIASLKM
110 120 130 140 150
DKRDAMVGNY TCEVTELSRE GKTVIELKNR TVSWFSPNEK ILIVIFPILA
160 170 180 190 200
ILLFWGKFGI LTLKYKSSHT NKRIILLLVA GLVLTVIVVV GAILLIPGEK
210 220 230 240 250
PVKNASGLGL IVISTGILIL LQYNVFMTAF GMTSFTIAIL ITQVLGYVLA
260 270 280 290 300
LVGLCLCIMA CEPVHGPLLI SGLGIIALAE LLGLVYMKFV ASNQRTIQPP

RNR
Length:303
Mass (Da):33,098
Last modified:September 27, 2005 - v2
Checksum:i87C0E6EF758FFF58
GO
Isoform 2 (identifier: Q61735-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     131-131: T → TAFNTDQGSACSYEEEKGGCKL

Show »
Length:324
Mass (Da):35,317
Checksum:iF6447B75543BBF17
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei131 – 1311T → TAFNTDQGSACSYEEEKGGC KL in isoform 2. 3 PublicationsVSP_015793

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z25524 mRNA. Translation: CAA80978.1.
AB012693 mRNA. Translation: BAA25401.1.
BC009094 mRNA. Translation: AAH09094.1.
BC012667 mRNA. Translation: AAH12667.1.
CCDSiCCDS28212.1. [Q61735-2]
PIRiS36646.
RefSeqiNP_034711.1. NM_010581.3. [Q61735-2]
XP_006521874.1. XM_006521811.2. [Q61735-1]
UniGeneiMm.31752.
Mm.390865.

Genome annotation databases

EnsembliENSMUST00000084838; ENSMUSP00000099853; ENSMUSG00000055447. [Q61735-2]
GeneIDi16423.
KEGGimmu:16423.
UCSCiuc007zkg.1. mouse. [Q61735-2]
uc007zkh.1. mouse. [Q61735-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z25524 mRNA. Translation: CAA80978.1.
AB012693 mRNA. Translation: BAA25401.1.
BC009094 mRNA. Translation: AAH09094.1.
BC012667 mRNA. Translation: AAH12667.1.
CCDSiCCDS28212.1. [Q61735-2]
PIRiS36646.
RefSeqiNP_034711.1. NM_010581.3. [Q61735-2]
XP_006521874.1. XM_006521811.2. [Q61735-1]
UniGeneiMm.31752.
Mm.390865.

3D structure databases

ProteinModelPortaliQ61735.
SMRiQ61735. Positions 19-134.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200835. 2 interactions.
IntActiQ61735. 5 interactions.
MINTiMINT-1711744.

PTM databases

PhosphoSiteiQ61735.
SwissPalmiQ61735.

Proteomic databases

EPDiQ61735.
MaxQBiQ61735.
PeptideAtlasiQ61735.
PRIDEiQ61735.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000084838; ENSMUSP00000099853; ENSMUSG00000055447. [Q61735-2]
GeneIDi16423.
KEGGimmu:16423.
UCSCiuc007zkg.1. mouse. [Q61735-2]
uc007zkh.1. mouse. [Q61735-1]

Organism-specific databases

CTDi961.
MGIiMGI:96617. Cd47.

Phylogenomic databases

GeneTreeiENSGT00390000007697.
HOGENOMiHOG000013020.
HOVERGENiHBG003808.
InParanoidiQ61735.
KOiK06266.
OMAiVYMKFVA.
OrthoDBiEOG79GT78.
PhylomeDBiQ61735.
TreeFamiTF336026.

Enzyme and pathway databases

ReactomeiR-MMU-202733. Cell surface interactions at the vascular wall.
R-MMU-216083. Integrin cell surface interactions.
R-MMU-391160. Signal regulatory protein (SIRP) family interactions.

Miscellaneous databases

ChiTaRSiCd47. mouse.
PROiQ61735.
SOURCEiSearch...

Gene expression databases

BgeeiQ61735.
ExpressionAtlasiQ61735. baseline and differential.
GenevisibleiQ61735. MM.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
InterProiIPR006704. CD47.
IPR013147. CD47_TM.
IPR013270. CD47_Vset.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
[Graphical view]
PANTHERiPTHR10613. PTHR10613. 1 hit.
PfamiPF04549. CD47. 1 hit.
PF08204. V-set_CD47. 1 hit.
[Graphical view]
PROSITEiPS50835. IG_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of integrin-associated protein: an immunoglobulin family member with multiple membrane spanning domains implicated in alpha-v beta-3-dependent ligand binding."
    Lindberg F.P., Gresham H.D., Schwarz E., Brown E.J.
    J. Cell Biol. 123:485-496(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), SUBCELLULAR LOCATION.
    Tissue: Pre-B cell.
  2. "Integrin-associated protein (IAP, also termed CD47) is involved in stroma-supported erythropoiesis."
    Furusawa T., Yanai N., Hara T., Miyajima A., Obinata M.
    J. Biochem. 123:101-106(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    Strain: C57BL/6J.
    Tissue: Stromal cell.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 83-303 (ISOFORM 2).
    Strain: FVB/N.
    Tissue: Mammary gland and Salivary gland.
  4. Lubec G., Kang S.U.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 86-99, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: C57BL/6J.
    Tissue: Brain.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Kidney, Lung, Spleen and Testis.

Entry informationi

Entry nameiCD47_MOUSE
AccessioniPrimary (citable) accession number: Q61735
Secondary accession number(s): Q921Z2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 27, 2005
Last sequence update: September 27, 2005
Last modified: July 6, 2016
This is version 126 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.