Reviewed, UniProtKB/Swiss-Prot Q61730 (IL1AP_MOUSE)

Names and origin

Protein names

Recommended name:
    Interleukin-1 receptor accessory protein
      Short name=IL-1 receptor accessory protein
      Short name=IL-1RAcP

Gene names

Name:

Il1rap

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	570 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Mediates interleukin-1-dependent activation of NF-kappa-B. Isoform 1 is part of the membrane-bound form of the IL-1 receptor. Signaling involves formation of a ternary complex containing IL1R1, TOLLIP, MYD88, and IRAK1 or IRAK2. Isoform 2 modulates the response to interleukins by associating with soluble IL1R1 and enhancing interleukin-binding to the decoy receptor. Ref.5
Subcellular location	Isoform 1: Cell membrane; Single-pass type I membrane protein Ref.1. Isoform 2: Secreted Ref.1.
Tissue specificity	Detected in lung, brain, spleen, thymus and liver. Ref.1
Sequence similarities	Belongs to the interleukin-1 receptor family. Contains 3 Ig-like C2-type (immunoglobulin-like) domains. Contains 1 TIR domain.

Ontologies

Keywords
Cellular component	Cell membrane Membrane Secreted
Coding sequence diversity	Alternative splicing
Domain	Immunoglobulin domain Repeat Signal Transmembrane
Molecular function	Receptor
PTM	Disulfide bond Glycoprotein Phosphoprotein
Gene Ontology (GO)
Biological process	cytokine-mediated signaling pathway Ref.5 Inferred from direct assay. Source: MGI innate immune response Inferred from electronic annotation. Source: InterPro interleukin-2 biosynthetic process Ref.5 Inferred from direct assay. Source: MGI
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	interleukin-1 receptor activity Ref.5 Inferred from direct assay. Source: MGI
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Tollip	Q9QZ06	1	EBI-525035,EBI-74272

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q61730-1)

Also known as: MuIL-1R AcP;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q61730-2)

Also known as: SmuIL-1R AcP;

The sequence of this isoform differs from the canonical sequence as follows:
     351-359: VIPPRYTVE → GNGCTEPMTL
     360-570: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Chain	21 – 570	550	Interleukin-1 receptor accessory protein		PRO_0000015452
Regions
	Topological domain	21 – 367	347	Extracellular Potential
	Transmembrane	368 – 388	21	Potential
	Topological domain	389 – 570	182	Cytoplasmic Potential
	Domain	21 – 128	108	Ig-like C2-type 1
	Domain	139 – 230	92	Ig-like C2-type 2
	Domain	243 – 348	106	Ig-like C2-type 3
	Domain	403 – 549	147	TIR
Amino acid modifications
	Modified residue	395	1	Phosphothreonine Ref.7
	Modified residue	398	1	Phosphothreonine Ref.7
	Glycosylation	57	1	N-linked (GlcNAc...) Potential
	Glycosylation	107	1	N-linked (GlcNAc...) Potential
	Glycosylation	111	1	N-linked (GlcNAc...) Potential
	Glycosylation	118	1	N-linked (GlcNAc...) Potential
	Glycosylation	196	1	N-linked (GlcNAc...) Potential
	Glycosylation	209	1	N-linked (GlcNAc...) Potential
	Glycosylation	299	1	N-linked (GlcNAc...) Potential
	Disulfide bond	47 ↔ 114		Potential
	Disulfide bond	137 ↔ 181		Potential
	Disulfide bond	160 ↔ 212		Potential
	Disulfide bond	266 ↔ 332		Potential
Natural variations
	Alternative sequence	351 – 359	9	VIPPRYTVE → GNGCTEPMTL in isoform 2.		VSP_008054
	Alternative sequence	360 – 570	211	Missing in isoform 2.		VSP_008055
Experimental info
	Mutagenesis	527 – 534	8	KGEKSKYP → AAAAAAAA: Abolishes interaction with MYD88 and IL-1-dependent activation of NF-kappa-B. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (MuIL-1R AcP) [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 4D4B07E0310AFDC5	FASTA	570	65,741
	10 20 30 40 50 60 MGLLWYLMSL SFYGILQSHA SERCDDWGLD TMRQIQVFED EPARIKCPLF EHFLKYNYST 70 80 90 100 110 120 AHSSGLTLIW YWTRQDRDLE EPINFRLPEN RISKEKDVLW FRPTLLNDTG NYTCMLRNTT 130 140 150 160 170 180 YCSKVAFPLE VVQKDSCFNS AMRFPVHKMY IEHGIHKITC PNVDGYFPSS VKPSVTWYKG 190 200 210 220 230 240 CTEIVDFHNV LPEGMNLSFF IPLVSNNGNY TCVVTYPENG RLFHLTRTVT VKVVGSPKDA 250 260 270 280 290 300 LPPQIYSPND RVVYEKEPGE ELVIPCKVYF SFIMDSHNEV WWTIDGKKPD DVTVDITINE 310 320 330 340 350 360 SVSYSSTEDE TRTQILSIKK VTPEDLRRNY VCHARNTKGE AEQAAKVKQK VIPPRYTVEL 370 380 390 400 410 420 ACGFGATVFL VVVLIVVYHV YWLEMVLFYR AHFGTDETIL DGKEYDIYVS YARNVEEEEF 430 440 450 460 470 480 VLLTLRGVLE NEFGYKLCIF DRDSLPGGIV TDETLSFIQK SRRLLVVLSP NYVLQGTQAL 490 500 510 520 530 540 LELKAGLENM ASRGNINVIL VQYKAVKDMK VKELKRAKTV LTVIKWKGEK SKYPQGRFWK 550 560 570 QLQVAMPVKK SPRWSSNDKQ GLSYSSLKNV
	Isoform 2 (SmuIL-1R AcP). Checksum: E525471759FF7719	FASTA	360	41,567
	10 20 30 40 50 60 MGLLWYLMSL SFYGILQSHA SERCDDWGLD TMRQIQVFED EPARIKCPLF EHFLKYNYST 70 80 90 100 110 120 AHSSGLTLIW YWTRQDRDLE EPINFRLPEN RISKEKDVLW FRPTLLNDTG NYTCMLRNTT 130 140 150 160 170 180 YCSKVAFPLE VVQKDSCFNS AMRFPVHKMY IEHGIHKITC PNVDGYFPSS VKPSVTWYKG 190 200 210 220 230 240 CTEIVDFHNV LPEGMNLSFF IPLVSNNGNY TCVVTYPENG RLFHLTRTVT VKVVGSPKDA 250 260 270 280 290 300 LPPQIYSPND RVVYEKEPGE ELVIPCKVYF SFIMDSHNEV WWTIDGKKPD DVTVDITINE 310 320 330 340 350 360 SVSYSSTEDE TRTQILSIKK VTPEDLRRNY VCHARNTKGE AEQAAKVKQK GNGCTEPMTL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and characterization of a second subunit of the interleukin 1 receptor complex." Greenfeder S.A., Nunes P., Kwee L., Labow M., Chizzonite R.A., Ju G. J. Biol. Chem. 270:13757-13765(1995) [PubMed: 7775431] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), INTERACTION WITH IL1R1, TISSUE SPECIFICITY, SUBCELLULAR LOCATION. Tissue: Fibroblast.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: C57BL/6J. Tissue: Brain and Spinal cord.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Liver.
[4]	"Tollip, a new component of the IL-1R1 pathway, links IRAK to the IL-1 receptor." Burns K., Clatworthy J., Martin L., Martinon F., Plumpton C., Maschera B., Lewis A., Ray K., Tschopp J., Volpe F. Nat. Cell Biol. 2:346-351(2000) [PubMed: 10854325] [Abstract] Cited for: INTERACTION WITH TOLLIP.
[5]	"Identification of essential regions in the cytoplasmic tail of interleukin-1 receptor accessory protein critical for interleukin-1 signaling." Radons J., Gabler S., Wesche H., Korherr C., Hofmeister R., Falk W. J. Biol. Chem. 277:16456-16463(2002) [PubMed: 11880380] [Abstract] Cited for: MUTAGENESIS OF 527-LYS--PRO-534, INTERACTION WITH MYD88, FUNCTION.
[6]	"Characterization of a cascade of protein interactions initiated at the IL-1 receptor." Boch J.A., Yoshida Y., Koyama Y., Wara-Aswapati N., Peng H., Unlu S., Auron P.E. Biochem. Biophys. Res. Commun. 303:525-531(2003) [PubMed: 12659850] [Abstract] Cited for: INTERACTION WITH IRAK2.
[7]	"A differential phosphoproteomic analysis of retinoic acid-treated P19 cells." Smith J.C., Duchesne M.A., Tozzi P., Ethier M., Figeys D. J. Proteome Res. 6:3174-3186(2007) [PubMed: 17622165] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-395 AND THR-398, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X85999 mRNA. Translation: CAA59991.1. AK039582 mRNA. Translation: BAC30392.1. AK045686 mRNA. Translation: BAC32457.1. BC021159 mRNA. Translation: AAH21159.1.
IPI	IPI00113879. IPI00124823.
PIR	A57535.
RefSeq	NP_032390.1. NP_598864.1.
UniGene	Mm.253424
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-296N.
IntAct	Q61730. 3 interactions.
STRING	Q61730.
PTM databases
PhosphoSite	Q61730.
Proteomic databases
PRIDE	Q61730.
Genome annotation databases
Ensembl	ENSMUST00000023156; ENSMUSP00000023156; ENSMUSG00000022514; Mus musculus. [Genome view] ENSMUST00000096129; ENSMUSP00000093843; ENSMUSG00000022514; Mus musculus. [Genome view]
GeneID	16180.
UCSC	uc007yvd.1. mouse. uc007yve.1. mouse.
Organism-specific databases
CTD	16180.
MGI	MGI:104975. Il1rap.
Phylogenomic databases
HOVERGEN	Q61730.
OMA	VDGFFPS.
Gene expression databases
ArrayExpress	Q61730.
Bgee	Q61730.
CleanEx	MM_IL1RAP.
Genevestigator	Q61730.
GermOnline	ENSMUSG00000022514. Mus musculus.
Family and domain databases
InterPro	IPR013151. Ig. IPR007110. Ig-like. IPR013783. Ig-like_fold. IPR003599. Ig_sub. IPR015621. IL1-receptor. IPR004075. IL1_rcpt_1. IPR004074. IL1_rcpt_I/II. IPR000157. Toll-Interleukin_rcpt. [Graphical view]
Gene3D	G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
PANTHER	PTHR11890. IL1-receptor. 1 hit.
Pfam	PF00047. ig. 3 hits. PF01582. TIR. 1 hit. [Graphical view]
PRINTS	PR01536. INTRLKN1R12F. PR01537. INTRLKN1R1F.
SMART	SM00409. IG. 3 hits. SM00255. TIR. 1 hit. [Graphical view]
PROSITE	PS50835. IG_LIKE. 2 hits. PS50104. TIR. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	289053.
SOURCE	Search...

Entry information

Entry name

IL1AP_MOUSE

Accession

Primary (citable) accession number: Q61730
Secondary accession number(s): Q8VCB9

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 22, 2003
Last sequence update:	November 1, 1996
Last modified:	December 15, 2009
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)