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Protein

Bone sialoprotein 2

Gene

Ibsp

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Promotes Arg-Gly-Asp-dependent cell attachment (By similarity).By similarity

GO - Biological processi

  • bone mineralization Source: MGI
  • cell adhesion Source: UniProtKB
  • cellular response to growth factor stimulus Source: MGI
  • extracellular matrix organization Source: MGI
  • osteoblast differentiation Source: MGI
Complete GO annotation...

Keywords - Biological processi

Biomineralization, Cell adhesion

Keywords - Ligandi

Sialic acid

Enzyme and pathway databases

ReactomeiR-MMU-216083. Integrin cell surface interactions.

Names & Taxonomyi

Protein namesi
Recommended name:
Bone sialoprotein 2
Alternative name(s):
Bone sialoprotein II
Short name:
BSP II
Cell-binding sialoprotein
Integrin-binding sialoprotein
Gene namesi
Name:Ibsp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:96389. Ibsp.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 16By similarityAdd BLAST16
ChainiPRO_000002033117 – 324Bone sialoprotein 2Add BLAST308

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei31PhosphoserineBy similarity1
Modified residuei67PhosphoserineBy similarity1
Modified residuei75PhosphoserineBy similarity1
Modified residuei76PhosphoserineBy similarity1
Modified residuei95PhosphoserineBy similarity1
Glycosylationi107N-linked (GlcNAc...)Sequence analysis1
Modified residuei155PhosphoserineBy similarity1
Glycosylationi183N-linked (GlcNAc...)Sequence analysis1
Glycosylationi188N-linked (GlcNAc...)Sequence analysis1
Glycosylationi196N-linked (GlcNAc...)Sequence analysis1
Modified residuei320SulfotyrosineBy similarity1
Modified residuei321SulfotyrosineBy similarity1

Post-translational modificationi

N-glycosylated; glycans consist of sialylated and core-fucosylated bi-, tri- and tetraantennary chains.By similarity
Sulfated on either Tyr-320 or Tyr-321.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein, Sulfation

Proteomic databases

PaxDbiQ61711.
PRIDEiQ61711.

PTM databases

PhosphoSitePlusiQ61711.

Expressioni

Gene expression databases

BgeeiENSMUSG00000029306.
CleanExiMM_IBSP.
GenevisibleiQ61711. MM.

Interactioni

Protein-protein interaction databases

IntActiQ61711. 1 interactor.
MINTiMINT-8283231.
STRINGi10090.ENSMUSP00000031246.

Structurei

3D structure databases

ProteinModelPortaliQ61711.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi293 – 295Cell attachment siteSequence analysis3

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi68 – 179Asp/Glu-rich (acidic)Add BLAST112
Compositional biasi77 – 84Poly-Glu8
Compositional biasi157 – 172Poly-GluAdd BLAST16

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1181. Eukaryota.
ENOG4111M3T. LUCA.
GeneTreeiENSGT00390000002485.
HOGENOMiHOG000234824.
HOVERGENiHBG007981.
InParanoidiQ61711.
KOiK06253.
OMAiLYKHAYF.
OrthoDBiEOG091G0UXJ.
TreeFamiTF338678.

Family and domain databases

InterProiIPR008412. BSP_II.
[Graphical view]
PANTHERiPTHR10345. PTHR10345. 1 hit.
PfamiPF05432. BSP_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q61711-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTALILLSI LGMACAFSMK NFHRRIKAED SEENGVFKYR PRYFLYKHAY
60 70 80 90 100
FYPPLKRFPV QGGSDSSEEN GDGDSSEEEG EEEETSNEEE NNEDSEGNED
110 120 130 140 150
QEAEAENSTL STLSGVTASY GAETTPQAQT FELAALQLPK KAGDAESRAP
160 170 180 190 200
KVKESDEEEE EEEEEEENEN EEAEVDENEL AVNGTSTNST EVDGGNGSSG
210 220 230 240 250
GDNGEEAEAE EASVTEAGAE GTTGGRELTS VGTQTAVLLN GFQQTTPPPE
260 270 280 290 300
AYGTTSPPIR KSSTVEYGGE YEQTGNEYNN EYEVYDNENG EPRGDTYRAY
310 320
EDEYSYYKGH GYEGYEGQNY YYHQ
Length:324
Mass (Da):35,734
Last modified:July 27, 2011 - v2
Checksum:iD0CD12EA82D27773
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti73G → C in AAA37326 (Ref. 2) Curated1
Sequence conflicti108S → A in AAA21726 (PubMed:8180469).Curated1
Sequence conflicti108S → A in AAA37326 (Ref. 2) Curated1
Sequence conflicti259I → V in AAA37326 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L20232 mRNA. Translation: AAA21726.1.
L23801 mRNA. Translation: AAA37326.1.
AK132371 mRNA. Translation: BAE21132.1.
BC045143 mRNA. Translation: AAH45143.1.
CH466529 Genomic DNA. Translation: EDL20226.1.
CCDSiCCDS19485.1.
PIRiI49768.
RefSeqiNP_032344.2. NM_008318.3.
UniGeneiMm.4987.

Genome annotation databases

EnsembliENSMUST00000031246; ENSMUSP00000031246; ENSMUSG00000029306.
GeneIDi15891.
KEGGimmu:15891.
UCSCiuc008ykg.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L20232 mRNA. Translation: AAA21726.1.
L23801 mRNA. Translation: AAA37326.1.
AK132371 mRNA. Translation: BAE21132.1.
BC045143 mRNA. Translation: AAH45143.1.
CH466529 Genomic DNA. Translation: EDL20226.1.
CCDSiCCDS19485.1.
PIRiI49768.
RefSeqiNP_032344.2. NM_008318.3.
UniGeneiMm.4987.

3D structure databases

ProteinModelPortaliQ61711.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ61711. 1 interactor.
MINTiMINT-8283231.
STRINGi10090.ENSMUSP00000031246.

PTM databases

PhosphoSitePlusiQ61711.

Proteomic databases

PaxDbiQ61711.
PRIDEiQ61711.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000031246; ENSMUSP00000031246; ENSMUSG00000029306.
GeneIDi15891.
KEGGimmu:15891.
UCSCiuc008ykg.2. mouse.

Organism-specific databases

CTDi3381.
MGIiMGI:96389. Ibsp.

Phylogenomic databases

eggNOGiKOG1181. Eukaryota.
ENOG4111M3T. LUCA.
GeneTreeiENSGT00390000002485.
HOGENOMiHOG000234824.
HOVERGENiHBG007981.
InParanoidiQ61711.
KOiK06253.
OMAiLYKHAYF.
OrthoDBiEOG091G0UXJ.
TreeFamiTF338678.

Enzyme and pathway databases

ReactomeiR-MMU-216083. Integrin cell surface interactions.

Miscellaneous databases

PROiQ61711.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000029306.
CleanExiMM_IBSP.
GenevisibleiQ61711. MM.

Family and domain databases

InterProiIPR008412. BSP_II.
[Graphical view]
PANTHERiPTHR10345. PTHR10345. 1 hit.
PfamiPF05432. BSP_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSIAL_MOUSE
AccessioniPrimary (citable) accession number: Q61711
Secondary accession number(s): Q61363, Q80VR6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

It is possible that the segments of clustered carboxyl groups mediate the strong binding to hydroxyapatite.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.