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Protein

Inter-alpha-trypsin inhibitor heavy chain H3

Gene

Itih3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May act as a carrier of hyaluronan in serum or as a binding protein between hyaluronan and other matrix protein, including those on cell surfaces in tissues to regulate the localization, synthesis and degradation of hyaluronan which are essential to cells undergoing biological processes.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Serine protease inhibitor

Enzyme and pathway databases

ReactomeiR-MMU-114608. Platelet degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Inter-alpha-trypsin inhibitor heavy chain H3
Short name:
ITI heavy chain H3
Short name:
ITI-HC3
Short name:
Inter-alpha-inhibitor heavy chain 3
Gene namesi
Name:Itih3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:96620. Itih3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Sequence analysisAdd
BLAST
Propeptidei22 – 3312By similarityPRO_0000016535Add
BLAST
Chaini34 – 649616Inter-alpha-trypsin inhibitor heavy chain H3PRO_0000016536Add
BLAST
Propeptidei650 – 889240By similarityPRO_0000016537Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi91 – 911N-linked (GlcNAc...)Sequence analysis
Glycosylationi580 – 5801N-linked (GlcNAc...)1 Publication
Modified residuei649 – 6491Aspartate 1-(chondroitin 4-sulfate)-esterBy similarity

Post-translational modificationi

Heavy chains are linked to bikunin via chondroitin 4-sulfate esterified to the alpha-carboxyl of the C-terminal aspartate after propeptide cleavage.By similarity

Keywords - PTMi

Cleavage on pair of basic residues, Glycoprotein, Proteoglycan

Proteomic databases

MaxQBiQ61704.
PaxDbiQ61704.
PRIDEiQ61704.

PTM databases

PhosphoSiteiQ61704.

Expressioni

Tissue specificityi

Expressed in both liver and brain.

Gene expression databases

BgeeiQ61704.
CleanExiMM_ITIH3.
ExpressionAtlasiQ61704. baseline and differential.
GenevisibleiQ61704. MM.

Interactioni

Subunit structurei

I-alpha-I plasma protease inhibitors are assembled from one or two heavy chains (H1, H2 or H3) and one light chain, bikunin. Inter-alpha-inhibitor (I-alpha-I) is composed of H1, H2 and bikunin, inter-alpha-like inhibitor (I-alpha-LI) of H2 and bikunin, and pre-alpha-inhibitor (P-alpha-I) of H3 and bikunin.

Protein-protein interaction databases

IntActiQ61704. 3 interactions.
MINTiMINT-4099088.
STRINGi10090.ENSMUSP00000006697.

Structurei

3D structure databases

ProteinModelPortaliQ61704.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini29 – 158130VITPROSITE-ProRule annotationAdd
BLAST
Domaini284 – 467184VWFAPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the ITIH family.Curated
Contains 1 VIT domain.PROSITE-ProRule annotation
Contains 1 VWFA domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IEJB. Eukaryota.
COG2304. LUCA.
GeneTreeiENSGT00550000074468.
HOGENOMiHOG000000680.
HOVERGENiHBG057734.
InParanoidiQ61704.
OMAiFVVVLHQ.
OrthoDBiEOG7CRTP5.
TreeFamiTF328982.

Family and domain databases

Gene3Di3.40.50.410. 1 hit.
InterProiIPR010600. ITI_HC_C.
IPR013694. VIT.
IPR002035. VWF_A.
[Graphical view]
PfamiPF06668. ITI_HC_C. 1 hit.
PF08487. VIT. 1 hit.
PF00092. VWA. 1 hit.
[Graphical view]
SMARTiSM00609. VIT. 1 hit.
SM00327. VWA. 1 hit.
[Graphical view]
SUPFAMiSSF53300. SSF53300. 1 hit.
PROSITEiPS51468. VIT. 1 hit.
PS50234. VWFA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q61704-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRTMWWPCLV LALLSGLETS GFPRSPLQLL GKRSLPEGVV DGIEVYSTKI
60 70 80 90 100
SCKVTSRFAH NVVTTRAVNR ADTAKEVSFD VELPKTAFIT NFTLTIDGVT
110 120 130 140 150
YPGNVKEKEV AQKQYEKAVS QGKTAGLVKA SGRKLEKFTV SVNVAAGSKV
160 170 180 190 200
TFELTYEELL KRNKGKYEMY LKVQPKQLVR HFEIDAHIFE PQGISMLDAE
210 220 230 240 250
ASFITNDLLG SALTKSFSGK KGHVSFKPSL DQQRSCPTCT DSLLNGDFTI
260 270 280 290 300
VYDVNRESPG NVQIVNGYFV HFFAPQGLPV VPKNIVFVID VSGSMSGRKI
310 320 330 340 350
QQTREALLKI LDDVKEDDYL NFILFSTDVT TWKDHLVQAT PANLKEAKTF
360 370 380 390 400
VKNIHDQSMT NINDGLLKGI EMLNKAREDH TVPERSTSII IMLTDGDANT
410 420 430 440 450
GESRPEKIQE NVRNAIGGKF PLYNLGFGNN LNYNFLETLA LENHGLARRI
460 470 480 490 500
YEDSDANLQL QGFYEEVANP LLTNVEVEYP ENAILDLTRN SYPHFYDGSE
510 520 530 540 550
IVVAGRLVDR NMDNFKADVK GHGALNDLTF TEEVDMEEMD AALKEQGYIF
560 570 580 590 600
GDYIERLWAY LTIEQLLEKR KNAKGDEKEN ITAEALDLSL KYHFVTPLTS
610 620 630 640 650
MVVTKPEDNE DQTSIADKPG EEAIAETTTM SFLTTQQSSQ SPYYYVDGDP
660 670 680 690 700
HFIIQIPGKN DSICFNIDEK PGTVLRLIQD PVTGITVTGQ IIGDKRSNAS
710 720 730 740 750
SRTGKTYFGK LGITNAWMDF RVEVTTEKII LGTGAELSTF SWLDTITVTQ
760 770 780 790 800
TGLSVTINRK KNMVVSFGDG ISFVIILHQV WKKHPVHQDF LGFYVVDSHR
810 820 830 840 850
MSAQTHGLLG QFFQPFDFKV FGIRPGSDPT KPDATMVVKN HRLTVTRGSQ
860 870 880
KDYRKDASVG TKVICWFVHN NGEGLIDGVH TDYIVPSLF
Length:889
Mass (Da):99,358
Last modified:July 27, 2011 - v3
Checksum:iCB3FF5F5062A27B9
GO

Sequence cautioni

The sequence AAH15276.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence CAA49843.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti3 – 31T → A in CAA49843 (PubMed:7534067).Curated
Sequence conflicti28 – 281Q → R in CAA49843 (PubMed:7534067).Curated
Sequence conflicti28 – 281Q → R in AAH15276 (PubMed:15489334).Curated
Sequence conflicti618 – 6192KP → NA in CAA49843 (PubMed:7534067).Curated
Sequence conflicti624 – 6241I → F in CAA49843 (PubMed:7534067).Curated
Sequence conflicti746 – 7461I → V in CAA49843 (PubMed:7534067).Curated
Sequence conflicti746 – 7461I → V in AAH15276 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK146700 mRNA. No translation available.
CT025528 Genomic DNA. No translation available.
X70393 mRNA. Translation: CAA49843.1. Different initiation.
BC015276 mRNA. Translation: AAH15276.1. Different initiation.
CCDSiCCDS49434.1.
PIRiS54355.
RefSeqiNP_032433.2. NM_008407.2.
UniGeneiMm.4517.

Genome annotation databases

EnsembliENSMUST00000006697; ENSMUSP00000006697; ENSMUSG00000006522.
GeneIDi16426.
KEGGimmu:16426.
UCSCiuc033goy.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK146700 mRNA. No translation available.
CT025528 Genomic DNA. No translation available.
X70393 mRNA. Translation: CAA49843.1. Different initiation.
BC015276 mRNA. Translation: AAH15276.1. Different initiation.
CCDSiCCDS49434.1.
PIRiS54355.
RefSeqiNP_032433.2. NM_008407.2.
UniGeneiMm.4517.

3D structure databases

ProteinModelPortaliQ61704.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ61704. 3 interactions.
MINTiMINT-4099088.
STRINGi10090.ENSMUSP00000006697.

PTM databases

PhosphoSiteiQ61704.

Proteomic databases

MaxQBiQ61704.
PaxDbiQ61704.
PRIDEiQ61704.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000006697; ENSMUSP00000006697; ENSMUSG00000006522.
GeneIDi16426.
KEGGimmu:16426.
UCSCiuc033goy.1. mouse.

Organism-specific databases

CTDi3699.
MGIiMGI:96620. Itih3.

Phylogenomic databases

eggNOGiENOG410IEJB. Eukaryota.
COG2304. LUCA.
GeneTreeiENSGT00550000074468.
HOGENOMiHOG000000680.
HOVERGENiHBG057734.
InParanoidiQ61704.
OMAiFVVVLHQ.
OrthoDBiEOG7CRTP5.
TreeFamiTF328982.

Enzyme and pathway databases

ReactomeiR-MMU-114608. Platelet degranulation.

Miscellaneous databases

PROiQ61704.
SOURCEiSearch...

Gene expression databases

BgeeiQ61704.
CleanExiMM_ITIH3.
ExpressionAtlasiQ61704. baseline and differential.
GenevisibleiQ61704. MM.

Family and domain databases

Gene3Di3.40.50.410. 1 hit.
InterProiIPR010600. ITI_HC_C.
IPR013694. VIT.
IPR002035. VWF_A.
[Graphical view]
PfamiPF06668. ITI_HC_C. 1 hit.
PF08487. VIT. 1 hit.
PF00092. VWA. 1 hit.
[Graphical view]
SMARTiSM00609. VIT. 1 hit.
SM00327. VWA. 1 hit.
[Graphical view]
SUPFAMiSSF53300. SSF53300. 1 hit.
PROSITEiPS51468. VIT. 1 hit.
PS50234. VWFA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The three heavy-chain precursors for the inter-alpha-inhibitor family in mouse: new members of the multicopper oxidase protein group with differential transcription in liver and brain."
    Chan P., Risler J.-L., Raguenez G., Salier J.-P.
    Biochem. J. 306:505-512(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 3-889.
    Strain: C57BL/6N.
    Tissue: Liver.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 3-889.
    Strain: FVB/N.
    Tissue: Liver.
  5. "Proteome-wide characterization of N-glycosylation events by diagonal chromatography."
    Ghesquiere B., Van Damme J., Martens L., Vandekerckhove J., Gevaert K.
    J. Proteome Res. 5:2438-2447(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-580.
    Strain: C57BL/6J.
    Tissue: Plasma.
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Spleen and Testis.

Entry informationi

Entry nameiITIH3_MOUSE
AccessioniPrimary (citable) accession number: Q61704
Secondary accession number(s): E9QME9, Q91WG9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 27, 2011
Last modified: June 8, 2016
This is version 123 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.