Q61701 (ELAV4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ELAV-like protein 4

Alternative name(s):
Hu-antigen D
Short name=HuD
Paraneoplastic encephalomyelitis antigen HuD

Gene names

Name:	Elavl4
Synonyms:	Hud

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	385 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May play a role in neuron-specific RNA processing. Protects CDKN1A mRNA from decay by binding to its 3'-UTR. Binds to AU-rich sequences (AREs) of target mRNAs, including VEGF and FOS mRNA By similarity.
Subunit structure	Component of a TAU mRNP complex, at least composed of IGF2BP1, ELAVL4 and G3BP. Ref.4
Tissue specificity	Expressed in brain and testis. Ref.1
Post-translational modification	Methylation at Arg-248 by CARM1 weakens protective binding to the 3'-UTR of CDKN1A mRNA and down-regulates CDKN1A protein expression, thereby maintaining cells in a proliferative state. Methylation is inhibited by NGF, which facilitates neurite outgrowth By similarity.
Sequence similarities	Belongs to the RRM elav family. Contains 3 RRM (RNA recognition motif) domains.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Domain	Repeat
Ligand	RNA-binding
PTM	Methylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular_function	AU-rich element binding Inferred from sequence or structural similarity. Source: UniProtKB nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist.

Isoform 1 (identifier: Q61701-1)

Also known as: Long;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q61701-2)

Also known as: Short;

The sequence of this isoform differs from the canonical sequence as follows:
     251-277: Missing.

Isoform 3 (identifier: Q61701-3)

The sequence of this isoform is not available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 385	385	ELAV-like protein 4		PRO_0000081584
Regions
	Domain	51 – 129	79	RRM 1
	Domain	137 – 217	81	RRM 2
	Domain	302 – 380	79	RRM 3
Amino acid modifications
	Modified residue	248	1	Omega-N-methylated arginine; by CARM1 By similarity
Natural variations
	Alternative sequence	251 – 277	27	Missing in isoform 2.		VSP_005792

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (Long) [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 1EA9CDAB7E9BC683	FASTA	385	42,368
	10 20 30 40 50 60 MEWNGLKMII STMEPQVSNG PTSNTSNGPS SNNRNCPSPM QTGAATDDSK TNLIVNYLPQ 70 80 90 100 110 120 NMTQEEFRSL FGSIGEIESC KLVRDKITGQ SLGYGFVNYI DPKDAEKAIN TLNGLRLQTK 130 140 150 160 170 180 TIKVSYARPS SASIRDANLY VSGLPKTMTQ KELEQLFSQY GRIITSRILV DQVTGVSRGV 190 200 210 220 230 240 GFIRFDKRIE AEEAIKGLNG QKPSGATEPI TVKFANNPSQ KSSQALLSQL YQSPNRRYPG 250 260 270 280 290 300 PLHHQAQRFR LDNLLNMAYG VKRLMSGPVP PSACPPRFSP ITIDGMTSLV GMNIPGHTGT 310 320 330 340 350 360 GWCIFVYNLS PDSDESVLWQ LFGPFGAVNN VKVIRDFNTN KCKGFGFVTM TNYDEAAMAI 370 380 ASLNGYRLGD RVLQVSFKTN KAHKS
	Isoform 2 (Short) [UniParc]. Checksum: BE2984EA2BBCD038	FASTA	358	39,489
	10 20 30 40 50 60 MEWNGLKMII STMEPQVSNG PTSNTSNGPS SNNRNCPSPM QTGAATDDSK TNLIVNYLPQ 70 80 90 100 110 120 NMTQEEFRSL FGSIGEIESC KLVRDKITGQ SLGYGFVNYI DPKDAEKAIN TLNGLRLQTK 130 140 150 160 170 180 TIKVSYARPS SASIRDANLY VSGLPKTMTQ KELEQLFSQY GRIITSRILV DQVTGVSRGV 190 200 210 220 230 240 GFIRFDKRIE AEEAIKGLNG QKPSGATEPI TVKFANNPSQ KSSQALLSQL YQSPNRRYPG 250 260 270 280 290 300 PLHHQAQRFR FSPITIDGMT SLVGMNIPGH TGTGWCIFVY NLSPDSDESV LWQLFGPFGA 310 320 330 340 350 VNNVKVIRDF NTNKCKGFGF VTMTNYDEAA MAIASLNGYR LGDRVLQVSF KTNKAHKS
	Isoform 3 (Sequence not available).		–	–

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Tissue-specific expression of the gene encoding a mouse RNA binding protein homologous to human HuD antigen." Abe R., Uyeno Y., Yamamoto K., Sakamoto H. DNA Res. 1:175-180(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY. Tissue: Brain.
[2]	"Gene organization and chromosome location of the neural-specific RNA binding protein Elavl4." Inman M.V., Levy S., Mock B.A., Owens G.C. Gene 208:139-145(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Strain: ICR.
[3]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[4]	"The insulin-like growth factor mRNA binding-protein IMP-1 and the Ras-regulatory protein G3BP associate with tau mRNA and HuD protein in differentiated P19 neuronal cells." Atlas R., Behar L., Elliott E., Ginzburg I. J. Neurochem. 89:613-626(2004) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN A MRNP COMPLEX WITH IGF2BP1 AND G3BP, INTERACTION WITH IGF2BP1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D31953 mRNA. Translation: BAA06723.1. AF041341 mRNA. Translation: AAC40080.1. AL627425 Genomic DNA. Translation: CAM19662.1.
IPI	IPI00229977. IPI00466120.
PIR	JC2298.
RefSeq	NP_034618.2. NM_010488.4.
UniGene	Mm.3970.
3D structure databases
ProteinModelPortal	Q61701.
SMR	Q61701. Positions 49-263, 301-381.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-4094262.
PTM databases
PhosphoSite	Q61701.
Proteomic databases
PaxDb	Q61701.
PRIDE	Q61701.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000106597; ENSMUSP00000102207; ENSMUSG00000028546.
GeneID	15572.
KEGG	mmu:15572.
UCSC	uc008ucw.1. mouse.
Organism-specific databases
CTD	1996.
MGI	MGI:107427. Elavl4.
Phylogenomic databases
eggNOG	COG0724.
GeneTree	ENSGT00560000077064.
HOGENOM	HOG000231162.
HOVERGEN	HBG002295.
InParanoid	A2A9R7.
KO	K13208.
OMA	SNSRNCP.
OrthoDB	EOG4JHCFV.
Gene expression databases
ArrayExpress	Q61701.
Bgee	Q61701.
Genevestigator	Q61701.
GermOnline	ENSMUSG00000028546. Mus musculus.
Family and domain databases
Gene3D	3.30.70.330. 3 hits.
InterPro	IPR006548. ELAD_HUD_SF. IPR002343. Hud_Sxl_RNA. IPR012677. Nucleotide-bd_a/b_plait. IPR000504. RRM_dom. [Graphical view]
Pfam	PF00076. RRM_1. 3 hits. [Graphical view]
PRINTS	PR00961. HUDSXLRNA.
SMART	SM00360. RRM. 3 hits. [Graphical view]
TIGRFAMs	TIGR01661. ELAV_HUD_SF. 1 hit.
PROSITE	PS50102. RRM. 3 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	288560.
SOURCE	Search...

Entry information

Entry name

ELAV4_MOUSE

Accession

Primary (citable) accession number: Q61701
Secondary accession number(s): A2A9R7, O55010

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	May 29, 2013
This is version 97 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program