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Protein

Heat shock 70 kDa protein 1A

Gene

Hspa1a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. Essential for STUB1-mediated ubiquitination and degradation of FOXP3 in regulatory T-cells (Treg) during inflammation.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei71ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi12 – 15ATPBy similarity4
Nucleotide bindingi202 – 204ATPBy similarity3
Nucleotide bindingi268 – 275ATPBy similarity8
Nucleotide bindingi339 – 342ATPBy similarity4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Stress response

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-3371453. Regulation of HSF1-mediated heat shock response.
R-MMU-3371568. Attenuation phase.
R-MMU-3371571. HSF1-dependent transactivation.
R-MMU-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Heat shock 70 kDa protein 1A
Alternative name(s):
Heat shock 70 kDa protein 3
Short name:
HSP70.3
Hsp68
Gene namesi
Name:Hspa1a
Synonyms:Hsp70-3, Hsp70A1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:96244. Hspa1a.

Subcellular locationi

  • Cytoplasm By similarity

  • Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000782502 – 641Heat shock 70 kDa protein 1AAdd BLAST640

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei108N6-acetyllysineBy similarity1
Modified residuei246N6-acetyllysineBy similarity1
Modified residuei348N6-acetyllysineBy similarity1
Modified residuei469Omega-N-methylarginineBy similarity1
Modified residuei561N6,N6,N6-trimethyllysine; by METTL21A; alternateBy similarity1
Modified residuei561N6,N6-dimethyllysine; alternateBy similarity1
Modified residuei604PhosphoserineCombined sources1
Modified residuei631PhosphoserineBy similarity1
Modified residuei633PhosphoserineBy similarity1
Modified residuei636PhosphothreonineBy similarity1

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

EPDiQ61696.
MaxQBiQ61696.
PaxDbiQ61696.
PRIDEiQ61696.

2D gel databases

REPRODUCTION-2DPAGEQ61696.

PTM databases

iPTMnetiQ61696.
PhosphoSitePlusiQ61696.

Expressioni

Gene expression databases

BgeeiENSMUSG00000091971.
CleanExiMM_HSPA1A.
ExpressionAtlasiQ61696. baseline and differential.
GenevisibleiQ61696. MM.

Interactioni

Subunit structurei

Component of the CatSper complex (By similarity). Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs (By similarity). Interacts with CHCHD3, DNAJC7, IRAK1BP1, PPP5C and TSC2 (By similarity). Interacts with TERT; the interaction occurs in the absence of the RNA component, TERC, and dissociates once the TERT complex has formed (By similarity). Interacts with METTL21A (By similarity). Interacts with DNAAF2 (PubMed:19052621). Interacts with TRIM5 (via B30.2/SPRY domain) (By similarity). Interacts with PARK2 (By similarity). Interacts with FOXP3 (PubMed:23973223). Interacts with NOD2; the interaction enhances NOD2 stability (By similarity). Interacts with DNAJC9 (via J domain) (By similarity). Interacts with ATF5; the interaction protects ATF5 from degradation via proteasome-dependent and caspase-dependent processes (By similarity). Interacts with RNF207 (via the C-terminus); this interaction additively increases KCNH2 expression (By similarity).By similarity2 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi228756. 1 interactor.
IntActiQ61696. 5 interactors.
MINTiMINT-1866478.
STRINGi10090.ENSMUSP00000084586.

Structurei

3D structure databases

ProteinModelPortaliQ61696.
SMRiQ61696.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the heat shock protein 70 family.Curated

Phylogenomic databases

eggNOGiKOG0101. Eukaryota.
COG0443. LUCA.
GeneTreeiENSGT00860000133969.
HOGENOMiHOG000228135.
HOVERGENiHBG051845.
InParanoidiQ61696.
KOiK03283.
OMAiKTQIHDI.
OrthoDBiEOG091G03SF.
PhylomeDBiQ61696.
TreeFamiTF105042.

Family and domain databases

Gene3Di1.20.1270.10. 1 hit.
2.60.34.10. 1 hit.
InterProiIPR018181. Heat_shock_70_CS.
IPR029048. HSP70_C.
IPR029047. HSP70_peptide-bd.
IPR013126. Hsp_70_fam.
[Graphical view]
PfamiPF00012. HSP70. 1 hit.
[Graphical view]
PRINTSiPR00301. HEATSHOCK70.
SUPFAMiSSF100920. SSF100920. 1 hit.
SSF100934. SSF100934. 1 hit.
PROSITEiPS00297. HSP70_1. 1 hit.
PS00329. HSP70_2. 1 hit.
PS01036. HSP70_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q61696-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKNTAIGID LGTTYSCVGV FQHGKVEIIA NDQGNRTTPS YVAFTDTERL
60 70 80 90 100
IGDAAKNQVA LNPQNTVFDA KRLIGRKFGD AVVQSDMKHW PFQVVNDGDK
110 120 130 140 150
PKVQVNYKGE SRSFFPEEIS SMVLTKMKEI AEAYLGHPVT NAVITVPAYF
160 170 180 190 200
NDSQRQATKD AGVIAGLNVL RIINEPTAAA IAYGLDRTGK GERNVLIFDL
210 220 230 240 250
GGGTFDVSIL TIDDGIFEVK ATAGDTHLGG EDFDNRLVSH FVEEFKRKHK
260 270 280 290 300
KDISQNKRAV RRLRTACERA KRTLSSSTQA SLEIDSLFEG IDFYTSITRA
310 320 330 340 350
RFEELCSDLF RGTLEPVEKA LRDAKMDKAQ IHDLVLVGGS TRIPKVQKLL
360 370 380 390 400
QDFFNGRDLN KSINPDEAVA YGAAVQAAIL MGDKSENVQD LLLLDVAPLS
410 420 430 440 450
LGLETAGGVM TALIKRNSTI PTKQTQTFTT YSDNQPGVLI QVYEGERAMT
460 470 480 490 500
RDNNLLGRFE LSGIPPAPRG VPQIEVTFDI DANGILNVTA TDKSTGKANK
510 520 530 540 550
ITITNDKGRL SKEEIERMVQ EAERYKAEDE VQRDRVAAKN ALESYAFNMK
560 570 580 590 600
SAVEDEGLKG KLSEADKKKV LDKCQEVISW LDSNTLADKE EFVHKREELE
610 620 630 640
RVCSPIISGL YQGAGAPGAG GFGAQAPKGA SGSGPTIEEV D
Length:641
Mass (Da):70,079
Last modified:December 21, 2004 - v2
Checksum:iF49C33E602EAE334
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti221 – 225ATAGD → TDGRT in AAA57234 (PubMed:2868009).Curated5
Sequence conflicti229G → E in AAA57234 (PubMed:2868009).Curated1
Sequence conflicti233F → V in AAA57234 (PubMed:2868009).Curated1
Sequence conflicti238 – 244VSHFVEE → EDLREQ in AAA57234 (PubMed:2868009).Curated7
Sequence conflicti295T → R in AAA57234 (PubMed:2868009).Curated1
Sequence conflicti332H → Q in AAA57234 (PubMed:2868009).Curated1
Sequence conflicti342R → A in AAA57233 (PubMed:8076831).Curated1
Sequence conflicti409V → G in AAA57235 (PubMed:2868009).Curated1
Sequence conflicti413 – 416LIKR → RHQA in AAA57234 (PubMed:2868009).Curated4
Sequence conflicti413 – 416LIKR → RHQA in AAA57235 (PubMed:2868009).Curated4
Sequence conflicti494S → T in AAH54782 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M76613 Genomic DNA. Translation: AAA57233.1.
AF109906 Genomic DNA. Translation: AAC84169.1.
BC054782 mRNA. Translation: AAH54782.1.
M12571 mRNA. Translation: AAA57234.1.
M12572 mRNA. Translation: AAA57235.1.
CCDSiCCDS50080.1.
PIRiA26283.
RefSeqiNP_034609.2. NM_010479.2.
UniGeneiMm.6388.

Genome annotation databases

EnsembliENSMUST00000087328; ENSMUSP00000084586; ENSMUSG00000091971.
GeneIDi193740.
KEGGimmu:193740.
UCSCiuc008cep.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M76613 Genomic DNA. Translation: AAA57233.1.
AF109906 Genomic DNA. Translation: AAC84169.1.
BC054782 mRNA. Translation: AAH54782.1.
M12571 mRNA. Translation: AAA57234.1.
M12572 mRNA. Translation: AAA57235.1.
CCDSiCCDS50080.1.
PIRiA26283.
RefSeqiNP_034609.2. NM_010479.2.
UniGeneiMm.6388.

3D structure databases

ProteinModelPortaliQ61696.
SMRiQ61696.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi228756. 1 interactor.
IntActiQ61696. 5 interactors.
MINTiMINT-1866478.
STRINGi10090.ENSMUSP00000084586.

PTM databases

iPTMnetiQ61696.
PhosphoSitePlusiQ61696.

2D gel databases

REPRODUCTION-2DPAGEQ61696.

Proteomic databases

EPDiQ61696.
MaxQBiQ61696.
PaxDbiQ61696.
PRIDEiQ61696.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000087328; ENSMUSP00000084586; ENSMUSG00000091971.
GeneIDi193740.
KEGGimmu:193740.
UCSCiuc008cep.1. mouse.

Organism-specific databases

CTDi3303.
MGIiMGI:96244. Hspa1a.

Phylogenomic databases

eggNOGiKOG0101. Eukaryota.
COG0443. LUCA.
GeneTreeiENSGT00860000133969.
HOGENOMiHOG000228135.
HOVERGENiHBG051845.
InParanoidiQ61696.
KOiK03283.
OMAiKTQIHDI.
OrthoDBiEOG091G03SF.
PhylomeDBiQ61696.
TreeFamiTF105042.

Enzyme and pathway databases

ReactomeiR-MMU-3371453. Regulation of HSF1-mediated heat shock response.
R-MMU-3371568. Attenuation phase.
R-MMU-3371571. HSF1-dependent transactivation.
R-MMU-6798695. Neutrophil degranulation.

Miscellaneous databases

PROiQ61696.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000091971.
CleanExiMM_HSPA1A.
ExpressionAtlasiQ61696. baseline and differential.
GenevisibleiQ61696. MM.

Family and domain databases

Gene3Di1.20.1270.10. 1 hit.
2.60.34.10. 1 hit.
InterProiIPR018181. Heat_shock_70_CS.
IPR029048. HSP70_C.
IPR029047. HSP70_peptide-bd.
IPR013126. Hsp_70_fam.
[Graphical view]
PfamiPF00012. HSP70. 1 hit.
[Graphical view]
PRINTSiPR00301. HEATSHOCK70.
SUPFAMiSSF100920. SSF100920. 1 hit.
SSF100934. SSF100934. 1 hit.
PROSITEiPS00297. HSP70_1. 1 hit.
PS00329. HSP70_2. 1 hit.
PS01036. HSP70_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHS71A_MOUSE
AccessioniPrimary (citable) accession number: Q61696
Secondary accession number(s): Q61697, Q7TQD8, Q9QWJ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: December 21, 2004
Last modified: November 30, 2016
This is version 140 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.